Rh2DG102100

Belongs to the RNase T2 family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr2D
OG0019650
Physical Location & Seq
Forward (+)
8272297 .. 8273325
1029 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh2DG102100.1

Sequence Viewer

Length: 567 bp
ATGATTGATAGCATCTTAAAAGGCAACAAAGATGATTTGGAAAGGTATTGGCCAAACTTGAAGCACACAAAGTTCGATGAGAGTAAAAAATTCTGGATTAGTGAGTGGATTAAACATGGTAGCTGCTCCGCAAAGACTCCTGCTAATTACTTGAGCCTGGTTTTTGATCTAATGAAGAAAATCAAGAAATTTGATGTGAAAAAGATATTTGAAAAGCACGGAATTTTACCAAGTGATCAGAGAGTGTATCAGGTGCAAGATTTTGCACAGGCAATATTTGACGAAACTAAATTCTGGACAGGGATTGAATGCATATCGAACGTCAACGGTAAGGGGCAGCATCTATTTCAAATTTATTTCTGCTTAACAGCTCAAGCAAAATTCAAGAATTGTTCGAACGATCGTGCTTTCTATAAAAACTGCCAATGGTCTTCGACAGTGTCATCGACAGTCACCTTCCCCTTGCCTCCGCCCAGTCACATGGTATCTGCAAATGAAGAAGAGAGAAGCAATGCTGCCCAGAAGCTTCTAAAGGGAGAAGATAGAGATGTAAAAAGCAAGAAGTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Ribonuclease_T2

Protein Analysis

188

Amino Acids

21.8

Weight (kDa)

9.18

Isoelectric Point (pI)

30.12

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Ribonuclease_T2 PF00445 7 - 131 5.4e-23 Ribonuclease T2 family
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0019650)

Species Orthologous Gene IDs
fragaria_vesca FvH4_1g10040
rosa_chinensis RchiOBHm_Chr2g0095681
rosa_laevigata RLG00000016585
rosa_multiflora Rmu_sc0001309.1_g000009 Rmu_ssc0000215.1_g000027
rosa_samantha Rh2AG108600 Rh2DG102100

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AciI CCGC 2 cut(s) 129, 470
AcoI YGGCCR 1 cut(s) 50
AcsI RAATTY 6 cut(s) 89, 188, 222, 290, 351, 380
AgsI TTSAA 5 cut(s) 61, 212, 308, 350, 385
AjnI CCWGG 1 cut(s) 156
AluBI AGCT 3 cut(s) 123, 371, 526
AluI AGCT 3 cut(s) 123, 371, 526
AoxI GGCC 1 cut(s) 50
ApeKI GCWGC 3 cut(s) 123, 337, 515
ApoI RAATTY 6 cut(s) 89, 188, 222, 290, 351, 380
AsuHPI GGTGA 1 cut(s) 445
AsuII TTCGAA 1 cut(s) 395
BalI TGGCCA 1 cut(s) 52
BbsI GAAGAC 1 cut(s) 423
BbvI GCAGC 3 cut(s) 110, 349, 502
BciT130I CCWGG 1 cut(s) 158
BclI TGATCA 1 cut(s) 235
BisI GCNGC 3 cut(s) 124, 338, 516
BlsI GCNGC 3 cut(s) 125, 339, 517
Bme1390I CCNGG 1 cut(s) 158
BmrFI CCNGG 1 cut(s) 158
BmrI ACTGGG 1 cut(s) 468
BmsI GCATC 2 cut(s) 21, 349
BmuI ACTGGG 1 cut(s) 468
BpiI GAAGAC 1 cut(s) 423
Bpu14I TTCGAA 1 cut(s) 395
BpuEI CTTGAG 2 cut(s) 172, 357
Bse1I ACTGG 1 cut(s) 474
Bse3DI GCAATG 1 cut(s) 517
BseBI CCWGG 1 cut(s) 158
BseMI GCAATG 1 cut(s) 517
BseNI ACTGG 1 cut(s) 474
BseXI GCAGC 3 cut(s) 110, 349, 502
Bsh1285I CGRYCG 1 cut(s) 403
BshFI GGCC 1 cut(s) 52
BsiEI CGRYCG 1 cut(s) 403
BsmI GAATGC 1 cut(s) 314
BsnI GGCC 1 cut(s) 52
Bsp119I TTCGAA 1 cut(s) 395
Bsp143I GATC 3 cut(s) 166, 235, 400
BspACI CCGC 2 cut(s) 129, 470
BspANI GGCC 1 cut(s) 52
BspT104I TTCGAA 1 cut(s) 395
BsrDI GCAATG 1 cut(s) 517
BsrI ACTGG 1 cut(s) 474
BssMI GATC 3 cut(s) 166, 235, 400
Bst2UI CCWGG 1 cut(s) 158
Bst4CI ACNGT 3 cut(s) 329, 439, 451
Bst6I CTCTTC 1 cut(s) 495
BstBI TTCGAA 1 cut(s) 395
BstKTI GATC 3 cut(s) 169, 238, 403
BstMBI GATC 3 cut(s) 166, 235, 400
BstMCI CGRYCG 1 cut(s) 403
BstNI CCWGG 1 cut(s) 158
BstSCI CCNGG 1 cut(s) 156
BstV1I GCAGC 3 cut(s) 110, 349, 502
BstV2I GAAGAC 1 cut(s) 423
BstXI CCANNNNNNTGG 1 cut(s) 481
BsuRI GGCC 1 cut(s) 52
BtsIMutI CAGTG 1 cut(s) 444
CviAII CATG 2 cut(s) 116, 481
CviJI RGCY 5 cut(s) 52, 123, 156, 371, 526
CviKI_1 RGCY 5 cut(s) 52, 123, 156, 371, 526
DpnI GATC 3 cut(s) 168, 237, 402
DpnII GATC 3 cut(s) 166, 235, 400
EaeI YGGCCR 1 cut(s) 50
Eam1104I CTCTTC 1 cut(s) 495
EarI CTCTTC 1 cut(s) 495
EciI GGCGGA 1 cut(s) 459
EcoRII CCWGG 1 cut(s) 156
EcoT22I ATGCAT 1 cut(s) 314
FaeI CATG 2 cut(s) 119, 484
FaiI YATR 4 cut(s) 117, 314, 414, 482
FatI CATG 2 cut(s) 115, 480
FbaI TGATCA 1 cut(s) 235
Fnu4HI GCNGC 3 cut(s) 124, 338, 516
Fsp4HI GCNGC 3 cut(s) 124, 338, 516
GluI GCNGC 3 cut(s) 124, 338, 516
HaeIII GGCC 1 cut(s) 52
Hin1II CATG 2 cut(s) 119, 484
HincII GTYRAC 1 cut(s) 325
HindII GTYRAC 1 cut(s) 325
HindIII AAGCTT 1 cut(s) 524
HinfI GANTC 1 cut(s) 136
HphI GGTGA 1 cut(s) 445
Hpy166II GTNNAC 1 cut(s) 325
Hpy188I TCNGA 1 cut(s) 240
Hpy188III TCNNGA 4 cut(s) 94, 184, 295, 385
Hpy8I GTNNAC 1 cut(s) 325
HpyAV CCTTC 1 cut(s) 466
HpyCH4III ACNGT 3 cut(s) 329, 439, 451
HpyCH4IV ACGT 1 cut(s) 321
HpyCH4V TGCA 4 cut(s) 256, 266, 312, 491
HpySE526I ACGT 1 cut(s) 321
Hsp92II CATG 2 cut(s) 119, 484
Ksp22I TGATCA 1 cut(s) 235
Kzo9I GATC 3 cut(s) 166, 235, 400
LmnI GCTCC 1 cut(s) 131
Lsp1109I GCAGC 3 cut(s) 110, 349, 502
LweI GCATC 2 cut(s) 21, 349
MaeII ACGT 1 cut(s) 321
MaeIII GTNAC 2 cut(s) 451, 476
MalI GATC 3 cut(s) 168, 237, 402
MboI GATC 3 cut(s) 166, 235, 400
MboII GAAGA 5 cut(s) 187, 423, 509, 512, 551
MlsI TGGCCA 1 cut(s) 52
MluCI AATT 8 cut(s) 89, 145, 188, 222, 290, 351, 380, 388
MluNI TGGCCA 1 cut(s) 52
MlyI GAGTC 1 cut(s) 130
MnlI CCTC 1 cut(s) 477
Mox20I TGGCCA 1 cut(s) 52
Mph1103I ATGCAT 1 cut(s) 314
MscI TGGCCA 1 cut(s) 52
MseI TTAA 3 cut(s) 17, 111, 365
Msp20I TGGCCA 1 cut(s) 52
MspR9I CCNGG 1 cut(s) 158
Mva1269I GAATGC 1 cut(s) 314
MvaI CCWGG 1 cut(s) 158
NdeII GATC 3 cut(s) 166, 235, 400
NlaIII CATG 2 cut(s) 119, 484
NmuCI GTSAC 2 cut(s) 451, 476
NsiI ATGCAT 1 cut(s) 314
NspV TTCGAA 1 cut(s) 395
PctI GAATGC 1 cut(s) 314
PflFI GACNNNGTC 1 cut(s) 439
PkrI GCNGC 3 cut(s) 125, 339, 517
Ple19I CGATCG 1 cut(s) 403
PleI GAGTC 1 cut(s) 130
PpsI GAGTC 1 cut(s) 130
Psp6I CCWGG 1 cut(s) 156
PspGI CCWGG 1 cut(s) 156
PsyI GACNNNGTC 1 cut(s) 439
PvuI CGATCG 1 cut(s) 403
SaqAI TTAA 3 cut(s) 17, 111, 365
SatI GCNGC 3 cut(s) 124, 338, 516
Sau3AI GATC 3 cut(s) 166, 235, 400
SchI GAGTC 1 cut(s) 130
ScrFI CCNGG 1 cut(s) 158
SetI ASST 7 cut(s) 47, 125, 255, 324, 373, 458, 528
SfaNI GCATC 2 cut(s) 21, 349
SfuI TTCGAA 1 cut(s) 395
SmlI CTYRAG 2 cut(s) 151, 372
SmoI CTYRAG 2 cut(s) 151, 372
Sse9I AATT 8 cut(s) 89, 145, 188, 222, 290, 351, 380, 388
SsiI CCGC 2 cut(s) 129, 470
SspI AATATT 1 cut(s) 276
StyD4I CCNGG 1 cut(s) 156
TaaI ACNGT 3 cut(s) 329, 439, 451
TaiI ACGT 1 cut(s) 324
TaqI TCGA 5 cut(s) 75, 317, 395, 434, 446
TasI AATT 8 cut(s) 89, 145, 188, 222, 290, 351, 380, 388
Tru1I TTAA 3 cut(s) 17, 111, 365
Tru9I TTAA 3 cut(s) 17, 111, 365
TscAI CASTG 1 cut(s) 444
TseFI GTSAC 2 cut(s) 451, 476
TseI GCWGC 3 cut(s) 123, 337, 515
Tsp45I GTSAC 2 cut(s) 451, 476
TspDTI ATGAA 2 cut(s) 188, 510
TspGWI ACGGA 1 cut(s) 234
TspRI CASTG 1 cut(s) 444
Tth111I GACNNNGTC 1 cut(s) 439
XapI RAATTY 6 cut(s) 89, 188, 222, 290, 351, 380
Zsp2I ATGCAT 1 cut(s) 314
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.