FvH4_1g20190

No description available

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb1
OG0015521
Physical Location & Seq
Forward (+)
12411701 .. 12413481
1781 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_1g20190.t1

Sequence Viewer

Length: 603 bp
ATGGCAACCATCAATGGCTTCCAGCTTAAAATGTCCAGCCTCATAAGCCAAGAACGAAAATCCCAAGTTCTGCATGGTACTAATTTTCTATGTCTCGATGAGATTCCTCATCCAAGATCATCATCAATATTCAACAAAGTTCCAAGAAAACTCAGCCATGATCACAGTCGACTGGTCACAAAGACGGCGTCTCATGCTGATAGCTCTCAATCAGAACCTCATCAAGTGCCGGCCAAATCCCCATTCTCTGCCTGGAAACATTGGATGGTGGGATTAACGTTAAGCATATTACTACCATCTTTGAGACACAAAGGGGGATCCTTCCTGGCCCTTAAAAAAAAGGTGGATGTGGCAATGGAAACCGCGGAGTCCGTGACAGAGGTGGTGGAAGAGTTAGCTGAGGAAGTAGAGAAGGTGTCTGAACAAGTAGCAGAGAAGCTACCTGATGACGCTAAGCTTAAGAAAGCTATGGAATCAGTTGAACATTTGGCCGAAGAAGCTGTGGAGAAAGCTAAGCTGGCCGAGGATATCATTCACAAAGTCAAGGAAGTGGAAGAAGAGGTGGAGGAGGCACTAATTAGTGGCTCAGAGAAGAAGAATTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

201

Amino Acids

22.24

Weight (kDa)

5.95

Isoelectric Point (pI)

52.63

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0015521)

Species Orthologous Gene IDs
fragaria_vesca FvH4_1g20190
malus_domestica MD15G1317800.v1.1
prunus_persica Prupe.6G194000_v2.0.a1 Prupe.6G194000_v2.0.a1
pyrus_communis pycom15g28040
rosa_chinensis RchiOBHm_Chr2g0112261
rosa_laevigata RLG00000017966
rosa_multiflora Rmu_sc0004467.1_g000024 Rmu_sc0011951.1_g000002
rosa_roxburghii Rroxscaffold_2G00131580
rosa_rugosa Rorug02G0179600
rosa_samantha Rh2AG232100 Rh2BG245400 Rh2CG236300 Rh2DG239800
rosa_wichuraiana Rw2G018000

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccI GTMKAC 1 cut(s) 169
AccII CGCG 1 cut(s) 365
AciI CCGC 2 cut(s) 363, 365
AclI AACGTT 1 cut(s) 278
AclWI GGATC 2 cut(s) 312, 325
AcoI YGGCCR 3 cut(s) 231, 489, 519
AcyI GRCGYC 1 cut(s) 188
AfaI GTAC 1 cut(s) 79
AflII CTTAAG 1 cut(s) 458
AgsI TTSAA 2 cut(s) 133, 482
AjnI CCWGG 2 cut(s) 251, 324
AjuI GAANNNNNNNTTGG 2 cut(s) 227, 259
AluBI AGCT 9 cut(s) 25, 204, 398, 439, 457, 467, 500, 512, 517
AluI AGCT 9 cut(s) 25, 204, 398, 439, 457, 467, 500, 512, 517
Alw26I GTCTC 3 cut(s) 98, 195, 298
AlwI GGATC 2 cut(s) 312, 325
AoxI GGCC 4 cut(s) 231, 327, 489, 519
ArsI GACNNNNNNTTYG 2 cut(s) 173, 205
AspS9I GGNCC 1 cut(s) 328
BamHI GGATCC 1 cut(s) 317
BbvCI CCTCAGC 1 cut(s) 399
BccI CCATC 3 cut(s) 17, 259, 304
BceAI ACGGC 1 cut(s) 201
BciT130I CCWGG 2 cut(s) 253, 326
BclI TGATCA 1 cut(s) 160
BcoDI GTCTC 3 cut(s) 98, 195, 298
BfrI CTTAAG 1 cut(s) 458
BlpI GCTNAGC 2 cut(s) 453, 513
Bme1390I CCNGG 2 cut(s) 253, 326
BmgT120I GGNCC 1 cut(s) 328
BmiI GGNNCC 1 cut(s) 319
BmrFI CCNGG 2 cut(s) 253, 326
Bpu10I CCTNAGC 1 cut(s) 399
Bpu1102I GCTNAGC 2 cut(s) 453, 513
BsaBI GATNNNNATC 1 cut(s) 121
BsaHI GRCGYC 1 cut(s) 188
BsaJI CCNNGG 2 cut(s) 363, 522
Bse118I RCCGGY 1 cut(s) 229
Bse1I ACTGG 1 cut(s) 177
Bse3DI GCAATG 1 cut(s) 360
Bse8I GATNNNNATC 1 cut(s) 121
BseBI CCWGG 2 cut(s) 253, 326
BseDI CCNNGG 2 cut(s) 363, 522
BseGI GGATG 3 cut(s) 109, 270, 352
BseJI GATNNNNATC 1 cut(s) 121
BseMI GCAATG 1 cut(s) 360
BseMII CTCAG 3 cut(s) 166, 390, 600
BseNI ACTGG 1 cut(s) 177
BseRI GAGGAG 1 cut(s) 581
Bsh1236I CGCG 1 cut(s) 365
BshFI GGCC 4 cut(s) 233, 329, 491, 521
BsiSI CCGG 1 cut(s) 230
BsmAI GTCTC 3 cut(s) 98, 195, 298
BsmBI CGTCTC 1 cut(s) 195
BsnI GGCC 4 cut(s) 233, 329, 491, 521
Bsp143I GATC 3 cut(s) 116, 160, 317
Bsp1720I GCTNAGC 2 cut(s) 453, 513
BspACI CCGC 2 cut(s) 363, 365
BspANI GGCC 4 cut(s) 233, 329, 491, 521
BspCNI CTCAG 3 cut(s) 165, 391, 599
BspFNI CGCG 1 cut(s) 365
BspLI GGNNCC 1 cut(s) 319
BspPI GGATC 2 cut(s) 312, 325
BspTI CTTAAG 1 cut(s) 458
BsrDI GCAATG 1 cut(s) 360
BsrFI RCCGGY 1 cut(s) 229
BsrI ACTGG 1 cut(s) 177
BssAI RCCGGY 1 cut(s) 229
BssECI CCNNGG 2 cut(s) 363, 522
BssMI GATC 3 cut(s) 116, 160, 317
BssNI GRCGYC 1 cut(s) 188
Bst2UI CCWGG 2 cut(s) 253, 326
Bst4CI ACNGT 1 cut(s) 167
Bst6I CTCTTC 2 cut(s) 384, 552
BstACI GRCGYC 1 cut(s) 188
BstAFI CTTAAG 1 cut(s) 458
BstC8I GCNNGC 2 cut(s) 231, 519
BstDEI CTNAG 5 cut(s) 152, 399, 453, 513, 586
BstDSI CCRYGG 1 cut(s) 363
BstF5I GGATG 3 cut(s) 109, 270, 352
BstFNI CGCG 1 cut(s) 365
BstKTI GATC 3 cut(s) 119, 163, 320
BstMAI GTCTC 3 cut(s) 98, 195, 298
BstMBI GATC 3 cut(s) 116, 160, 317
BstMWI GCNNNNNNNGC 4 cut(s) 45, 194, 497, 518
BstNI CCWGG 2 cut(s) 253, 326
BstSCI CCNGG 2 cut(s) 251, 324
BstUI CGCG 1 cut(s) 365
BstX2I RGATCY 1 cut(s) 317
BstYI RGATCY 1 cut(s) 317
BsuRI GGCC 4 cut(s) 233, 329, 491, 521
BtgI CCRYGG 1 cut(s) 363
BtsCI GGATG 3 cut(s) 109, 270, 352
Cac8I GCNNGC 2 cut(s) 231, 519
Cfr10I RCCGGY 1 cut(s) 229
Cfr13I GGNCC 1 cut(s) 328
Cfr42I CCGCGG 1 cut(s) 366
CseI GACGC 2 cut(s) 177, 458
Csp6I GTAC 1 cut(s) 78
CviAII CATG 3 cut(s) 74, 158, 194
CviQI GTAC 1 cut(s) 78
DdeI CTNAG 5 cut(s) 152, 399, 453, 513, 586
DpnI GATC 3 cut(s) 118, 162, 319
DpnII GATC 3 cut(s) 116, 160, 317
EaeI YGGCCR 3 cut(s) 231, 489, 519
Eam1104I CTCTTC 2 cut(s) 384, 552
EarI CTCTTC 2 cut(s) 384, 552
Eco32I GATATC 1 cut(s) 529
EcoRII CCWGG 2 cut(s) 251, 324
EcoRV GATATC 1 cut(s) 529
Esp3I CGTCTC 1 cut(s) 195
FaeI CATG 3 cut(s) 77, 161, 197
FaiI YATR 7 cut(s) 44, 75, 91, 159, 195, 287, 470
FatI CATG 3 cut(s) 73, 157, 193
FbaI TGATCA 1 cut(s) 160
FblI GTMKAC 1 cut(s) 169
FokI GGATG 3 cut(s) 96, 277, 359
HaeIII GGCC 4 cut(s) 233, 329, 491, 521
HapII CCGG 1 cut(s) 230
HgaI GACGC 2 cut(s) 177, 458
Hin1I GRCGYC 1 cut(s) 188
Hin1II CATG 3 cut(s) 77, 161, 197
HincII GTYRAC 1 cut(s) 170
HindII GTYRAC 1 cut(s) 170
HindIII AAGCTT 1 cut(s) 455
HinfI GANTC 3 cut(s) 103, 368, 473
HpaII CCGG 1 cut(s) 230
Hpy166II GTNNAC 1 cut(s) 170
Hpy188I TCNGA 3 cut(s) 214, 421, 589
Hpy188III TCNNGA 1 cut(s) 95
Hpy8I GTNNAC 1 cut(s) 170
HpyAV CCTTC 2 cut(s) 331, 406
HpyCH4III ACNGT 1 cut(s) 167
HpyCH4IV ACGT 1 cut(s) 278
HpyCH4V TGCA 1 cut(s) 73
HpyF10VI GCNNNNNNNGC 4 cut(s) 45, 194, 497, 518
HpyF3I CTNAG 5 cut(s) 152, 399, 453, 513, 586
HpySE526I ACGT 1 cut(s) 278
Hsp92I GRCGYC 1 cut(s) 188
Hsp92II CATG 3 cut(s) 77, 161, 197
KroI GCCGGC 1 cut(s) 229
KroNI GCCGGC 1 cut(s) 231
Ksp22I TGATCA 1 cut(s) 160
KspI CCGCGG 1 cut(s) 366
Kzo9I GATC 3 cut(s) 116, 160, 317
MaeII ACGT 1 cut(s) 278
MaeIII GTNAC 2 cut(s) 175, 373
MalI GATC 3 cut(s) 118, 162, 319
MboI GATC 3 cut(s) 116, 160, 317
MboII GAAGA 4 cut(s) 401, 506, 566, 569
MflI RGATCY 1 cut(s) 317
MluCI AATT 3 cut(s) 82, 576, 598
MlyI GAGTC 1 cut(s) 377
MnlI CCTC 9 cut(s) 50, 117, 228, 373, 394, 517, 553, 559, 562
MroNI GCCGGC 1 cut(s) 229
MseI TTAA 5 cut(s) 27, 275, 281, 333, 459
MspA1I CMGCKG 1 cut(s) 365
MspCI CTTAAG 1 cut(s) 458
MspI CCGG 1 cut(s) 230
MspR9I CCNGG 2 cut(s) 253, 326
MvaI CCWGG 2 cut(s) 253, 326
MvnI CGCG 1 cut(s) 365
MwoI GCNNNNNNNGC 4 cut(s) 45, 194, 497, 518
NaeI GCCGGC 1 cut(s) 231
NdeII GATC 3 cut(s) 116, 160, 317
NgoMIV GCCGGC 1 cut(s) 229
NlaIII CATG 3 cut(s) 77, 161, 197
NlaIV GGNNCC 1 cut(s) 319
NmeAIII GCCGAG 1 cut(s) 547
NmuCI GTSAC 2 cut(s) 175, 373
PdiI GCCGGC 1 cut(s) 231
PfeI GAWTC 2 cut(s) 103, 473
PflFI GACNNNGTC 1 cut(s) 187
PleI GAGTC 1 cut(s) 376
PpsI GAGTC 1 cut(s) 376
Psp1406I AACGTT 1 cut(s) 278
Psp6I CCWGG 2 cut(s) 251, 324
PspGI CCWGG 2 cut(s) 251, 324
PspN4I GGNNCC 1 cut(s) 319
PspPI GGNCC 1 cut(s) 328
PsuI RGATCY 1 cut(s) 317
PsyI GACNNNGTC 1 cut(s) 187
RsaI GTAC 1 cut(s) 79
RsaNI GTAC 1 cut(s) 78
SacII CCGCGG 1 cut(s) 366
SalI GTCGAC 1 cut(s) 168
SaqAI TTAA 5 cut(s) 27, 275, 281, 333, 459
Sau3AI GATC 3 cut(s) 116, 160, 317
Sau96I GGNCC 1 cut(s) 328
SchI GAGTC 1 cut(s) 377
ScrFI CCNGG 2 cut(s) 253, 326
Sfr303I CCGCGG 1 cut(s) 366
SgrBI CCGCGG 1 cut(s) 366
SmlI CTYRAG 1 cut(s) 458
SmoI CTYRAG 1 cut(s) 458
Sse9I AATT 3 cut(s) 82, 576, 598
SsiI CCGC 2 cut(s) 363, 365
SspI AATATT 1 cut(s) 129
StyD4I CCNGG 2 cut(s) 251, 324
TaaI ACNGT 1 cut(s) 167
TaiI ACGT 1 cut(s) 281
TaqI TCGA 2 cut(s) 96, 169
TasI AATT 3 cut(s) 82, 576, 598
TfiI GAWTC 2 cut(s) 103, 473
Tru1I TTAA 5 cut(s) 27, 275, 281, 333, 459
Tru9I TTAA 5 cut(s) 27, 275, 281, 333, 459
TseFI GTSAC 2 cut(s) 175, 373
Tsp45I GTSAC 2 cut(s) 175, 373
TspGWI ACGGA 1 cut(s) 361
Tth111I GACNNNGTC 1 cut(s) 187
Vha464I CTTAAG 1 cut(s) 458
XcmI CCANNNNNNNNNTGG 2 cut(s) 71, 249
XmiI GTMKAC 1 cut(s) 169
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.