RLG00000017966

No description available

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_laevigata
Chr4
OG0015521
Physical Location & Seq
Forward (+)
23803324 .. 23804474
1151 bp
Loading structure...
UTR
Exon/CDS
Intron
RLM00000017966

Sequence Viewer

Length: 603 bp
ATGGCAACCATCAATGGCTTCCACCTTCACATGTCCAGCCTCAGAAGCCAAGAACACAAGTCCCAAGTCTTGCATGGTACTAATTTGCTGTGTCTCCATGAGAGTACTCCTCATCCAAGATCATCATCATTCAACAAAGGTCCAAGAAAACCCAGCCATGATTACAGACTGATCACAAAGGCGACGTCTCATGCATCAGATAGTTCTCAACCAGAACCTCATCAAGTTCCGGCCAAATCCCCATTCTCTGCCTGGAAACATTGGATACTGGGATTAACGTTAAGCATATTAGTGCCATCTTTTAGACACAAAGGGGGACCCTTCCTAGCCCTTAAAAGCAAGGTGGATGTGGCGGTGGAAACGGTGGAGTCCGTGACAGAGGTTGTGGAAGAGTTAGCTGAGGAAGTAGAGAAGGTGTCTGAACAAGTAGCAGACAAGCTACCTGATGATGCTAAGCTTAAGAAAGCTGTGGAGTCAGTTGAACATTTGGCGGAAGAAGCAGTGGAGAAAGCTAAGCTAGCTGAGGATATCATTCACAAGGTAAAGGAAGTGGAGAAAGAGGTGGAGGAGGCACTAATTGATAGCTCAGACAAGAAGCCTTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

201

Amino Acids

22.18

Weight (kDa)

6.11

Isoelectric Point (pI)

50.3

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0015521)

Species Orthologous Gene IDs
fragaria_vesca FvH4_1g20190
malus_domestica MD15G1317800.v1.1
prunus_persica Prupe.6G194000_v2.0.a1 Prupe.6G194000_v2.0.a1
pyrus_communis pycom15g28040
rosa_chinensis RchiOBHm_Chr2g0112261
rosa_laevigata RLG00000017966
rosa_multiflora Rmu_sc0004467.1_g000024 Rmu_sc0011951.1_g000002
rosa_roxburghii Rroxscaffold_2G00131580
rosa_rugosa Rorug02G0179600
rosa_samantha Rh2AG232100 Rh2BG245400 Rh2CG236300 Rh2DG239800
rosa_wichuraiana Rw2G018000

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AatII GACGTC 1 cut(s) 188
AciI CCGC 2 cut(s) 353, 491
AclI AACGTT 1 cut(s) 278
AcoI YGGCCR 1 cut(s) 231
AcyI GRCGYC 1 cut(s) 185
AfaI GTAC 2 cut(s) 79, 106
AflII CTTAAG 1 cut(s) 458
AflIII ACRYGT 1 cut(s) 30
AgsI TTSAA 2 cut(s) 133, 482
AjnI CCWGG 1 cut(s) 251
AjuI GAANNNNNNNTTGG 2 cut(s) 227, 259
AluBI AGCT 8 cut(s) 398, 439, 457, 467, 512, 517, 521, 585
AluI AGCT 8 cut(s) 398, 439, 457, 467, 512, 517, 521, 585
Alw26I GTCTC 2 cut(s) 98, 192
AoxI GGCC 1 cut(s) 231
ArsI GACNNNNNNTTYG 2 cut(s) 170, 202
AspS9I GGNCC 2 cut(s) 140, 317
AsuNHI GCTAGC 1 cut(s) 517
AvaII GGWCC 2 cut(s) 140, 317
BbvCI CCTCAGC 2 cut(s) 399, 522
BccI CCATC 2 cut(s) 17, 304
BciT130I CCWGG 1 cut(s) 253
BciVI GTATCC 1 cut(s) 258
BclI TGATCA 1 cut(s) 171
BcoDI GTCTC 2 cut(s) 98, 192
BfaI CTAG 2 cut(s) 326, 518
BfrI CTTAAG 1 cut(s) 458
BfuI GTATCC 1 cut(s) 258
BlpI GCTNAGC 2 cut(s) 453, 513
BmcAI AGTACT 1 cut(s) 106
Bme1390I CCNGG 1 cut(s) 253
Bme18I GGWCC 2 cut(s) 140, 317
BmgT120I GGNCC 2 cut(s) 140, 317
BmiI GGNNCC 2 cut(s) 318, 319
BmrFI CCNGG 1 cut(s) 253
BmrI ACTGGG 1 cut(s) 278
BmsI GCATC 2 cut(s) 203, 439
BmtI GCTAGC 1 cut(s) 521
BmuI ACTGGG 1 cut(s) 278
BplI GAGNNNNNCTC 2 cut(s) 94, 126
Bpu10I CCTNAGC 2 cut(s) 399, 522
Bpu1102I GCTNAGC 2 cut(s) 453, 513
BsaBI GATNNNNATC 1 cut(s) 124
BsaHI GRCGYC 1 cut(s) 185
Bse1I ACTGG 1 cut(s) 273
Bse8I GATNNNNATC 1 cut(s) 124
BseBI CCWGG 1 cut(s) 253
BseGI GGATG 2 cut(s) 112, 352
BseJI GATNNNNATC 1 cut(s) 124
BseMII CTCAG 4 cut(s) 55, 390, 513, 600
BseNI ACTGG 1 cut(s) 273
BseRI GAGGAG 2 cut(s) 99, 581
BseYI CCCAGC 1 cut(s) 152
BshFI GGCC 1 cut(s) 233
BsiSI CCGG 1 cut(s) 230
BslFI GGGAC 2 cut(s) 46, 330
BsmAI GTCTC 2 cut(s) 98, 192
BsmBI CGTCTC 1 cut(s) 192
BsmFI GGGAC 2 cut(s) 46, 330
BsnI GGCC 1 cut(s) 233
Bsp143I GATC 2 cut(s) 119, 171
Bsp1720I GCTNAGC 2 cut(s) 453, 513
BspACI CCGC 2 cut(s) 353, 491
BspANI GGCC 1 cut(s) 233
BspCNI CTCAG 4 cut(s) 54, 391, 514, 599
BspLI GGNNCC 2 cut(s) 318, 319
BspOI GCTAGC 1 cut(s) 521
BspTI CTTAAG 1 cut(s) 458
BsrI ACTGG 1 cut(s) 273
BssMI GATC 2 cut(s) 119, 171
BssNI GRCGYC 1 cut(s) 185
Bst2UI CCWGG 1 cut(s) 253
Bst4CI ACNGT 1 cut(s) 364
Bst6I CTCTTC 1 cut(s) 384
BstACI GRCGYC 1 cut(s) 185
BstAFI CTTAAG 1 cut(s) 458
BstC8I GCNNGC 1 cut(s) 519
BstDEI CTNAG 7 cut(s) 41, 399, 453, 513, 522, 586, 600
BstF5I GGATG 2 cut(s) 112, 352
BstKTI GATC 2 cut(s) 122, 174
BstMAI GTCTC 2 cut(s) 98, 192
BstMBI GATC 2 cut(s) 119, 171
BstMWI GCNNNNNNNGC 3 cut(s) 45, 497, 518
BstNI CCWGG 1 cut(s) 253
BstNSI RCATGY 1 cut(s) 34
BstSCI CCNGG 1 cut(s) 251
BsuI GTATCC 1 cut(s) 258
BsuRI GGCC 1 cut(s) 233
BtsCI GGATG 2 cut(s) 112, 352
BtsI GCAGTG 1 cut(s) 507
BtsIMutI CAGTG 1 cut(s) 507
Cac8I GCNNGC 1 cut(s) 519
Cfr13I GGNCC 2 cut(s) 140, 317
Csp6I GTAC 2 cut(s) 78, 105
CviAII CATG 5 cut(s) 31, 74, 98, 158, 191
CviQI GTAC 2 cut(s) 78, 105
DdeI CTNAG 7 cut(s) 41, 399, 453, 513, 522, 586, 600
DpnI GATC 2 cut(s) 121, 173
DpnII GATC 2 cut(s) 119, 171
EaeI YGGCCR 1 cut(s) 231
Eam1104I CTCTTC 1 cut(s) 384
EarI CTCTTC 1 cut(s) 384
EciI GGCGGA 1 cut(s) 506
Eco32I GATATC 1 cut(s) 529
Eco47I GGWCC 2 cut(s) 140, 317
EcoO109I RGGNCCY 1 cut(s) 317
EcoRII CCWGG 1 cut(s) 251
EcoRV GATATC 1 cut(s) 529
EcoT22I ATGCAT 1 cut(s) 196
Esp3I CGTCTC 1 cut(s) 192
FaeI CATG 5 cut(s) 34, 77, 101, 161, 194
FaiI YATR 6 cut(s) 32, 75, 99, 159, 192, 287
FaqI GGGAC 2 cut(s) 46, 330
FatI CATG 5 cut(s) 30, 73, 97, 157, 190
FbaI TGATCA 1 cut(s) 171
FokI GGATG 2 cut(s) 99, 359
FspBI CTAG 2 cut(s) 326, 518
GsaI CCCAGC 1 cut(s) 156
HaeIII GGCC 1 cut(s) 233
HapII CCGG 1 cut(s) 230
Hin1I GRCGYC 1 cut(s) 185
Hin1II CATG 5 cut(s) 34, 77, 101, 161, 194
HindIII AAGCTT 1 cut(s) 455
HinfI GANTC 2 cut(s) 368, 473
HpaII CCGG 1 cut(s) 230
Hpy188I TCNGA 4 cut(s) 44, 199, 421, 589
Hpy99I CGWCG 1 cut(s) 187
HpyAV CCTTC 3 cut(s) 35, 331, 406
HpyCH4III ACNGT 1 cut(s) 364
HpyCH4IV ACGT 2 cut(s) 185, 278
HpyCH4V TGCA 2 cut(s) 73, 194
HpyF10VI GCNNNNNNNGC 3 cut(s) 45, 497, 518
HpyF3I CTNAG 7 cut(s) 41, 399, 453, 513, 522, 586, 600
HpySE526I ACGT 2 cut(s) 185, 278
Hsp92I GRCGYC 1 cut(s) 185
Hsp92II CATG 5 cut(s) 34, 77, 101, 161, 194
KflI GGGWCCC 1 cut(s) 317
Ksp22I TGATCA 1 cut(s) 171
Kzo9I GATC 2 cut(s) 119, 171
LpnPI CCDG 8 cut(s) 49, 166, 225, 238, 243, 254, 265, 456
LweI GCATC 2 cut(s) 203, 439
MaeI CTAG 2 cut(s) 326, 518
MaeII ACGT 2 cut(s) 185, 278
MaeIII GTNAC 1 cut(s) 373
MalI GATC 2 cut(s) 121, 173
MboI GATC 2 cut(s) 119, 171
MboII GAAGA 2 cut(s) 401, 506
MluCI AATT 2 cut(s) 82, 576
MlyI GAGTC 2 cut(s) 377, 482
MnlI CCTC 9 cut(s) 50, 120, 228, 373, 394, 517, 553, 559, 562
Mph1103I ATGCAT 1 cut(s) 196
MseI TTAA 4 cut(s) 275, 281, 333, 459
MslI CAYNNNNRTG 1 cut(s) 290
MspCI CTTAAG 1 cut(s) 458
MspI CCGG 1 cut(s) 230
MspR9I CCNGG 1 cut(s) 253
MvaI CCWGG 1 cut(s) 253
MwoI GCNNNNNNNGC 3 cut(s) 45, 497, 518
NdeII GATC 2 cut(s) 119, 171
NheI GCTAGC 1 cut(s) 517
NlaIII CATG 5 cut(s) 34, 77, 101, 161, 194
NlaIV GGNNCC 2 cut(s) 318, 319
NmuCI GTSAC 1 cut(s) 373
NsiI ATGCAT 1 cut(s) 196
NspI RCATGY 1 cut(s) 34
PciI ACATGT 1 cut(s) 30
PleI GAGTC 2 cut(s) 376, 481
PpsI GAGTC 2 cut(s) 376, 481
PpuMI RGGWCCY 1 cut(s) 317
PscI ACATGT 1 cut(s) 30
Psp1406I AACGTT 1 cut(s) 278
Psp5II RGGWCCY 1 cut(s) 317
Psp6I CCWGG 1 cut(s) 251
PspFI CCCAGC 1 cut(s) 152
PspGI CCWGG 1 cut(s) 251
PspN4I GGNNCC 2 cut(s) 318, 319
PspPI GGNCC 2 cut(s) 140, 317
PspPPI RGGWCCY 1 cut(s) 317
RsaI GTAC 2 cut(s) 79, 106
RsaNI GTAC 2 cut(s) 78, 105
RseI CAYNNNNRTG 1 cut(s) 290
SaqAI TTAA 4 cut(s) 275, 281, 333, 459
Sau3AI GATC 2 cut(s) 119, 171
Sau96I GGNCC 2 cut(s) 140, 317
ScaI AGTACT 1 cut(s) 106
SchI GAGTC 2 cut(s) 377, 482
ScrFI CCNGG 1 cut(s) 253
SfaNI GCATC 2 cut(s) 203, 439
SinI GGWCC 2 cut(s) 140, 317
SmiMI CAYNNNNRTG 1 cut(s) 290
SmlI CTYRAG 1 cut(s) 458
SmoI CTYRAG 1 cut(s) 458
Sse9I AATT 2 cut(s) 82, 576
SsiI CCGC 2 cut(s) 353, 491
SspMI CTAG 2 cut(s) 326, 518
StyD4I CCNGG 1 cut(s) 251
TaaI ACNGT 1 cut(s) 364
TaiI ACGT 2 cut(s) 188, 281
TasI AATT 2 cut(s) 82, 576
TatI WGTACW 1 cut(s) 104
Tru1I TTAA 4 cut(s) 275, 281, 333, 459
Tru9I TTAA 4 cut(s) 275, 281, 333, 459
TscAI CASTG 1 cut(s) 507
TseFI GTSAC 1 cut(s) 373
Tsp45I GTSAC 1 cut(s) 373
TspGWI ACGGA 1 cut(s) 361
TspRI CASTG 1 cut(s) 507
Vha464I CTTAAG 1 cut(s) 458
VpaK11BI GGWCC 2 cut(s) 140, 317
XceI RCATGY 1 cut(s) 34
XcmI CCANNNNNNNNNTGG 2 cut(s) 71, 249
XspI CTAG 2 cut(s) 326, 518
ZraI GACGTC 1 cut(s) 186
ZrmI AGTACT 1 cut(s) 106
Zsp2I ATGCAT 1 cut(s) 196
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.