FvH4_1g24460

Cation H( ) antiporter

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb1
OG0017951
Physical Location & Seq
Reverse (-)
16301098 .. 16305884
4787 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_1g24460.t1

Sequence Viewer

Length: 2616 bp
ATGGCGAAACAGGCATCGCCGAACGTGACTAATATTACGACTAATGGATCAGATAGTTCAATAGTATGCTATGCCCCAACCATGATTACCACCAATGGTATTTGGCAAGGAGATAACCCTTTAGACTATTCCCTCCCTCTCTTCATCTTGCAGTTGACTCTGGTCGTCGTCACCACTCGTCTTCTCGTCCTCATTTTCAAACCCTTCCGGCAACCTCGTGTCATTGCAGAAATCATGGGTGGAGTGTTATTAGGACCATCGGTGCTTGGACAGTATAAACCATTTGCGGACACAATATTCCCCATGAGAAGTGTGATGGTGATCGAGACCATGGCAAACGTAGGTCTCCTCTACTTCCTCTTTCTCGTCGGCTTGGAGATGGACATTGCCGTTATCCGCCGTATGGGGAAAAGAGCAGTTATCATTGCCGTTGCCGGTATGATCCTCCCCTTCGCCATTGGTGCCGCCTTCTCGTTCCTCATCCACGATAGAGGACAATCAATGAACCAAGGTACCTTCATCCTCTTCCTTGGCGTTGCCCTCTCTGTCACCGCCTTCCCGGTCCTTGCTCGAGTCCTCGCCGAGCTCAAGCTCGTCAACACTGAGCTCGGCAGGATCGCATTGTCCTCGGCATTGGTAAATGATATGTGTACTTGGGCACTCTTAGCTCTGGCCATTGCCCTAGCGGAAAATGACTCCACTTCTTTGGCTTCCCTCTACGTCATTTTGTCTAGCATTGCCTTTGTTTGCTTTTGCATATTCGTTGTCCGACCTGCTGTGTGCTGGATTATCCGAAGAACTCCGGAAGGAGAATCCTTCAGTGAGTTCTACATTTGCCTCATTCTCACCGGGGTCATGATCTCCGGATTCGTTACTGATGCCATAGGAACACATTCCGTTTTTGGGGCTTTCGTGTTTGGTTTAGTCATCCCTAATGGACCTCTTGGCGTTACTCTCATCGAGAAGCTGGAAGATTTTGTTTCGGGTCTCCTGCTTCCTCTTTTCTTTGCAATTAGCGGACTTAGAACCAATATTACTACGATCCAAGGAGTTGGAACTTGGGGCCTTCTTATACTTGTTATCTTTCTCGCTTGCATCGGAAAAATAGCCGGTACAGTCATTGTCTGCCTCTTTTACCAGATGCCAATTGATGAAGGATTCACACTAGGATTGCTTATGAACACAAAAGGACTTGTCGAATTGATTGTCCTCAATGTGGGTAAGGACCAGAAGGTTCTAGATGACGAGGCCTTTGCAATTATGGTGATTGTAGCTGTTGTTATGACTGGAATCATTACACCAATAGTAACAACAGTTTACAAGCCGGCGAGAAGGTCAATACCTTACAAACGAAGAACAATTCAAAGGTCAAAACCTGATGCCGAGTTGCGAGTACTTGTTTGTGTCCACACGCCCCGAAATGTGCCTACAATGATCAACCTATTAGAAGCCTCTTACCCCACGAAGAGATCTCCTTTGTGCGTCTATGTACTACATTTGGTTGAGCTCAGTGGTCGTGCATCTGCAATGCTCATTGTTCATAACACTCGGAAATCCGGTAGGCCAGCGCTAAATAGAACACAAGCTCAATCTGACCATATCATCAATGCGTTCGAGAACTACGAGCAACATGCCGGTTGTGTCTCGGTGCAGCCCTTGACTGCAATCTCACCATACTCTACAATGCACGAAGACATTTGCAACTTGGCAGAGGACAAACGTGTTGCCTTTCTCATTATTCCTTTCCACAAACAGCAAACGGTTGATGGTGGAATGGAAGCCATGAACCCGGCTTTTCGAACAATGAACCAAAATGTATTAGCAAATGCGCCATGCTCAGTCGGAATTCTAGTCGATAGAGGTCTCAATACCTCCAATCGCCTGACTACAAACCATGTATCTCACAATATAGTGGTACTTTTCTTCGGTGGACCGGATGACAGAGAGGCGTTGTCATATGCATGGAGGATGTCTGAACATCCAGGAATAAGCCTTACACTTATTCGTTTCATTCCTGGGCCGGATGTGGCGGAGCCGGTAGGGGTAGTGAAACCCGATCTTGATGCTGCAAACAACAACAACAATTCAAAGATATTGTGTGTCGAAACGGACGAGGGGTGGGAGAAGCAACTGGACGAGAAGTTGATAAAAGAGTTTAGGGTGCAACATATGAACGACGAATCAATTGTGTACGTGGAGAAGGTGGTGAACAATGGGGAGGAGACGGTGGCGGAGGTAAGGGCGATGGACAACGTTCATGACCTATTTATAGTCGGCAGAGGACAAGGCATGATCTCGCCGTTGACAGCCGGACTAACGGATTGGAGTGAGTGCCCGGAACTCGGTGCTATTGGGGATTTGCTTGCGTCTTCCGACTTCGCAACAACTGCATCGGTTTTGGTAGTGCAACAATTCGTTGGTGATGAAGGGAGTGTAGCACACGATCACGAGCTCGATCATGATCACAATCACGATGGGGGACTCGGAACACCACAACCGGTGGCACTCAGCCCAGCTGGAGGTGATCAATTCGGCAGCATGCATCAAAATGGGAACAAGAGGACTCAACCACCAATTAGGGCACATAATAGTAACCCTAACGTATTCAGCGCCTAA

Protein Analysis

872

Amino Acids

94.79

Weight (kDa)

5.74

Isoelectric Point (pI)

34.85

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Na_H_Exchanger PF00999 60 - 437 7.9e-66 Sodium/hydrogen exchanger, transmembrane
CHX17_2nd PF23256 492 - 627 3.5e-29 Plant cation/H(+) antiporter domain
CHX17_C PF23259 636 - 807 6.3e-20 Plant cation/H(+) antiporter, C-terminal domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0017951)

Species Orthologous Gene IDs
arabidopsis_thaliana AT2G13620
fragaria_vesca FvH4_1g24460
malus_domestica MD01G1005100.v1.1
prunus_persica Prupe.4G271900_v2.0.a1
rosa_chinensis RchiOBHm_Chr2g0121601
rosa_laevigata RLG00000018626
rosa_roxburghii Rroxscaffold_2G00122330
rosa_rugosa Rorug02G0233800
rosa_samantha Rh2BG301500 Rh2CG280300
rosa_wichuraiana Rw2G023310

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 781
Acc65I GGTACC 1 cut(s) 512
AccB1I GGYRCC 2 cut(s) 461, 512
AccIII TCCGGA 2 cut(s) 802, 863
AciI CCGC 8 cut(s) 287, 397, 465, 552, 686, 1017, 2030, 2231
AclI AACGTT 1 cut(s) 2253
AclWI GGATC 4 cut(s) 55, 436, 623, 1036
AcoI YGGCCR 1 cut(s) 672
AcsI RAATTY 1 cut(s) 1845
AcuI CTGAAG 1 cut(s) 802
AfaI GTAC 7 cut(s) 514, 652, 1114, 1395, 1491, 1917, 2192
AfeI AGCGCT 1 cut(s) 1569
AfiI CCNNNNNNNGG 5 cut(s) 403, 903, 1216, 2342, 2519
AflIII ACRYGT 1 cut(s) 1720
AgeI ACCGGT 1 cut(s) 2497
AgsI TTSAA 4 cut(s) 60, 199, 1364, 2088
AjnI CCWGG 2 cut(s) 1981, 2014
Alw21I GWGCWC 4 cut(s) 588, 609, 1509, 2454
Alw26I GTCTC 6 cut(s) 320, 350, 992, 1648, 1868, 2216
AlwI GGATC 4 cut(s) 55, 436, 623, 1036
Ama87I CYCGRG 1 cut(s) 570
Aor13HI TCCGGA 2 cut(s) 802, 863
Aor51HI AGCGCT 1 cut(s) 1569
AoxI GGCC 5 cut(s) 672, 1063, 1248, 1562, 2018
ApeKI GCWGC 3 cut(s) 1651, 2066, 2535
ApoI RAATTY 1 cut(s) 1845
ArsI GACNNNNNNTTYG 4 cut(s) 1191, 1223, 1235, 1267
AsiGI ACCGGT 1 cut(s) 2497
Asp700I GAANNNNTTC 1 cut(s) 892
Asp718I GGTACC 1 cut(s) 512
AspLEI GCGC 3 cut(s) 1570, 1831, 2612
AspS9I GGNCC 7 cut(s) 254, 562, 938, 1063, 1225, 1931, 2018
AsuC2I CCSGG 4 cut(s) 560, 850, 1790, 2336
AsuHPI GGTGA 9 cut(s) 163, 331, 541, 838, 1276, 1662, 2218, 2432, 2534
AsuII TTCGAA 1 cut(s) 1798
AvaI CYCGRG 1 cut(s) 570
AvaII GGWCC 5 cut(s) 254, 562, 938, 1225, 1931
BaeGI GKGCMC 3 cut(s) 661, 2336, 2584
BaeI ACNNNNGTAYC 2 cut(s) 496, 529
BalI TGGCCA 1 cut(s) 674
BanI GGYRCC 2 cut(s) 461, 512
BanII GRGCYC 4 cut(s) 588, 609, 1509, 2454
BauI CACGAG 2 cut(s) 216, 2447
BbsI GAAGAC 3 cut(s) 173, 1698, 2361
Bbv12I GWGCWC 4 cut(s) 588, 609, 1509, 2454
BbvI GCAGC 3 cut(s) 1663, 2053, 2547
BccI CCATC 6 cut(s) 265, 310, 373, 1760, 2239, 2468
BceAI ACGGC 4 cut(s) 374, 384, 413, 2284
BcgI CGANNNNNNTGC 8 cut(s) 609, 643, 1235, 1269, 1613, 1647, 2045, 2079
BciT130I CCWGG 2 cut(s) 1983, 2016
BclI TGATCA 3 cut(s) 1434, 2461, 2524
BcnI CCSGG 4 cut(s) 560, 850, 1790, 2336
BcoDI GTCTC 6 cut(s) 320, 350, 992, 1648, 1868, 2216
BfaI CTAG 5 cut(s) 683, 732, 1166, 1238, 1850
BfoI RGCGCY 2 cut(s) 1571, 2613
BfuAI ACCTGC 1 cut(s) 781
BglII AGATCT 1 cut(s) 1469
BisI GCNGC 4 cut(s) 465, 1652, 2067, 2536
BlsI GCNGC 4 cut(s) 466, 1653, 2068, 2537
BmcAI AGTACT 1 cut(s) 1395
Bme1390I CCNGG 6 cut(s) 560, 850, 1790, 1983, 2016, 2336
Bme18I GGWCC 5 cut(s) 254, 562, 938, 1225, 1931
BmeT110I CYCGRG 1 cut(s) 570
BmgT120I GGNCC 7 cut(s) 254, 562, 938, 1063, 1225, 1931, 2018
BmiI GGNNCC 4 cut(s) 463, 514, 1064, 2034
BmrFI CCNGG 6 cut(s) 560, 850, 1790, 1983, 2016, 2336
BmsI GCATC 9 cut(s) 23, 868, 1104, 1131, 1369, 1529, 2053, 2399, 2551
BoxI GACNNNNGTC 1 cut(s) 161
BpiI GAAGAC 3 cut(s) 173, 1698, 2361
BpmI CTGGAG 1 cut(s) 2538
Bpu14I TTCGAA 1 cut(s) 1798
BpuEI CTTGAG 1 cut(s) 572
BpuMI CCSGG 4 cut(s) 560, 850, 1790, 2336
BsaAI YACGTR 1 cut(s) 2194
BsaBI GATNNNNATC 3 cut(s) 320, 2460, 2466
BsaI GGTCTC 4 cut(s) 320, 350, 992, 1868
BsaJI CCNNGG 7 cut(s) 330, 508, 529, 627, 849, 1045, 2015
BsaWI WCCGGW 5 cut(s) 802, 863, 1556, 1933, 2497
Bsc4I CCNNNNNNNGG 5 cut(s) 403, 903, 1216, 2342, 2519
Bse118I RCCGGY 6 cut(s) 434, 1109, 1324, 1634, 2035, 2497
Bse1I ACTGG 2 cut(s) 1291, 2136
Bse3DI GCAATG 6 cut(s) 222, 384, 423, 675, 735, 1533
Bse8I GATNNNNATC 3 cut(s) 320, 2460, 2466
BseAI TCCGGA 2 cut(s) 802, 863
BseBI CCWGG 2 cut(s) 1983, 2016
BseDI CCNNGG 7 cut(s) 330, 508, 529, 627, 849, 1045, 2015
BseGI GGATG 7 cut(s) 480, 519, 927, 1942, 1974, 1978, 2029
BseJI GATNNNNATC 3 cut(s) 320, 2460, 2466
BseLI CCNNNNNNNGG 5 cut(s) 403, 903, 1216, 2342, 2519
BseMI GCAATG 6 cut(s) 222, 384, 423, 675, 735, 1533
BseMII CTCAG 4 cut(s) 594, 1522, 1851, 2521
BseNI ACTGG 2 cut(s) 1291, 2136
BseRI GAGGAG 2 cut(s) 338, 2234
BseSI GKGCMC 3 cut(s) 661, 2336, 2584
BseXI GCAGC 3 cut(s) 1663, 2053, 2547
BseYI CCCAGC 1 cut(s) 2512
BsgI GTGCAG 1 cut(s) 1670
BshFI GGCC 5 cut(s) 674, 1065, 1250, 1564, 2020
BshNI GGYRCC 2 cut(s) 461, 512
BshTI ACCGGT 1 cut(s) 2497
BsiHKAI GWGCWC 4 cut(s) 588, 609, 1509, 2454
BsiHKCI CYCGRG 1 cut(s) 570
BslFI GGGAC 1 cut(s) 2493
BslI CCNNNNNNNGG 5 cut(s) 403, 903, 1216, 2342, 2519
BsmAI GTCTC 6 cut(s) 320, 350, 992, 1648, 1868, 2216
BsmBI CGTCTC 1 cut(s) 2216
BsmFI GGGAC 1 cut(s) 2493
BsnI GGCC 5 cut(s) 674, 1065, 1250, 1564, 2020
Bso31I GGTCTC 4 cut(s) 320, 350, 992, 1868
BsoBI CYCGRG 1 cut(s) 570
Bsp119I TTCGAA 1 cut(s) 1798
Bsp1286I GDGCHC 7 cut(s) 588, 609, 661, 1509, 2336, 2454, 2584
Bsp13I TCCGGA 2 cut(s) 802, 863
Bsp19I CCATGG 1 cut(s) 330
BspACI CCGC 8 cut(s) 287, 397, 465, 552, 686, 1017, 2030, 2231
BspANI GGCC 5 cut(s) 674, 1065, 1250, 1564, 2020
BspCNI CTCAG 4 cut(s) 595, 1521, 1850, 2520
BspEI TCCGGA 2 cut(s) 802, 863
BspHI TCATGA 3 cut(s) 855, 2257, 2458
BspLI GGNNCC 4 cut(s) 463, 514, 1064, 2034
BspMI ACCTGC 1 cut(s) 781
BspPI GGATC 4 cut(s) 55, 436, 623, 1036
BspT104I TTCGAA 1 cut(s) 1798
BspT107I GGYRCC 2 cut(s) 461, 512
BspTNI GGTCTC 4 cut(s) 320, 350, 992, 1868
BsrDI GCAATG 6 cut(s) 222, 384, 423, 675, 735, 1533
BsrFI RCCGGY 6 cut(s) 434, 1109, 1324, 1634, 2035, 2497
BsrI ACTGG 2 cut(s) 1291, 2136
BssAI RCCGGY 6 cut(s) 434, 1109, 1324, 1634, 2035, 2497
BssECI CCNNGG 7 cut(s) 330, 508, 529, 627, 849, 1045, 2015
BssSI CACGAG 2 cut(s) 216, 2447
BssT1I CCWWGG 4 cut(s) 330, 508, 529, 1045
Bst2BI CACGAG 2 cut(s) 216, 2447
Bst2UI CCWGG 2 cut(s) 1983, 2016
Bst4CI ACNGT 5 cut(s) 273, 1117, 1315, 1762, 2227
Bst6I CTCTTC 3 cut(s) 146, 530, 1460
BstAPI GCANNNNNTGC 1 cut(s) 2387
BstBAI YACGTR 1 cut(s) 2194
BstBI TTCGAA 1 cut(s) 1798
BstC8I GCNNGC 5 cut(s) 1093, 1326, 1566, 2364, 2540
BstDEI CTNAG 6 cut(s) 603, 664, 1022, 1508, 1837, 2507
BstDSI CCRYGG 1 cut(s) 330
BstF5I GGATG 7 cut(s) 480, 519, 927, 1942, 1974, 1978, 2029
BstH2I RGCGCY 2 cut(s) 1571, 2613
BstHHI GCGC 3 cut(s) 1570, 1831, 2612
BstMAI GTCTC 6 cut(s) 320, 350, 992, 1648, 1868, 2216
BstMWI GCNNNNNNNGC 4 cut(s) 11, 461, 665, 2387
BstNI CCWGG 2 cut(s) 1983, 2016
BstNSI RCATGY 2 cut(s) 1634, 2542
BstPAI GACNNNNGTC 1 cut(s) 161
BstSCI CCNGG 6 cut(s) 558, 848, 1788, 1981, 2014, 2334
BstSLI GKGCMC 3 cut(s) 661, 2336, 2584
BstV1I GCAGC 3 cut(s) 1663, 2053, 2547
BstV2I GAAGAC 3 cut(s) 173, 1698, 2361
BstX2I RGATCY 1 cut(s) 1469
BstXI CCANNNNNNTGG 2 cut(s) 706, 1052
BstYI RGATCY 1 cut(s) 1469
BsuRI GGCC 5 cut(s) 674, 1065, 1250, 1564, 2020
BtgI CCRYGG 1 cut(s) 330
BtgZI GCGATG 1 cut(s) 2258
BtsCI GGATG 7 cut(s) 480, 519, 927, 1942, 1974, 1978, 2029
BtsIMutI CAGTG 3 cut(s) 600, 826, 1516
BveI ACCTGC 1 cut(s) 781
Cac8I GCNNGC 5 cut(s) 1093, 1326, 1566, 2364, 2540
CciI TCATGA 3 cut(s) 855, 2257, 2458
CfoI GCGC 3 cut(s) 1570, 1831, 2612
Cfr10I RCCGGY 6 cut(s) 434, 1109, 1324, 1634, 2035, 2497
Cfr13I GGNCC 7 cut(s) 254, 562, 938, 1063, 1225, 1931, 2018
CseI GACGC 2 cut(s) 1471, 2355
Csp6I GTAC 7 cut(s) 513, 651, 1113, 1394, 1490, 1916, 2191
CspAI ACCGGT 1 cut(s) 2497
CviQI GTAC 7 cut(s) 513, 651, 1113, 1394, 1490, 1916, 2191
DdeI CTNAG 6 cut(s) 603, 664, 1022, 1508, 1837, 2507
EaeI YGGCCR 1 cut(s) 672
Eam1104I CTCTTC 3 cut(s) 146, 530, 1460
EarI CTCTTC 3 cut(s) 146, 530, 1460
EciI GGCGGA 3 cut(s) 386, 2045, 2246
Ecl136II GAGCTC 4 cut(s) 586, 607, 1507, 2452
Eco130I CCWWGG 4 cut(s) 330, 508, 529, 1045
Eco147I AGGCCT 1 cut(s) 1250
Eco24I GRGCYC 4 cut(s) 588, 609, 1509, 2454
Eco31I GGTCTC 4 cut(s) 320, 350, 992, 1868
Eco47I GGWCC 5 cut(s) 254, 562, 938, 1225, 1931
Eco47III AGCGCT 1 cut(s) 1569
Eco53kI GAGCTC 4 cut(s) 586, 607, 1507, 2452
Eco57I CTGAAG 1 cut(s) 802
Eco88I CYCGRG 1 cut(s) 570
EcoICRI GAGCTC 4 cut(s) 586, 607, 1507, 2452
EcoO109I RGGNCCY 1 cut(s) 1063
EcoRI GAATTC 1 cut(s) 1845
EcoRII CCWGG 2 cut(s) 1981, 2014
EcoT14I CCWWGG 4 cut(s) 330, 508, 529, 1045
EcoT22I ATGCAT 2 cut(s) 1963, 2544
EcoT38I GRGCYC 4 cut(s) 588, 609, 1509, 2454
ErhI CCWWGG 4 cut(s) 330, 508, 529, 1045
Esp3I CGTCTC 1 cut(s) 2216
FaqI GGGAC 1 cut(s) 2493
FauNDI CATATG 2 cut(s) 1957, 2169
FbaI TGATCA 3 cut(s) 1434, 2461, 2524
Fnu4HI GCNGC 4 cut(s) 465, 1652, 2067, 2536
FokI GGATG 7 cut(s) 467, 506, 914, 1949, 1965, 1981, 2036
FriOI GRGCYC 4 cut(s) 588, 609, 1509, 2454
Fsp4HI GCNGC 4 cut(s) 465, 1652, 2067, 2536
FspBI CTAG 5 cut(s) 683, 732, 1166, 1238, 1850
GlaI GCGC 3 cut(s) 1569, 1830, 2611
GluI GCNGC 4 cut(s) 465, 1652, 2067, 2536
GsaI CCCAGC 1 cut(s) 2516
GsuI CTGGAG 1 cut(s) 2538
HaeII RGCGCY 2 cut(s) 1571, 2613
HaeIII GGCC 5 cut(s) 674, 1065, 1250, 1564, 2020
HgaI GACGC 2 cut(s) 1471, 2355
HhaI GCGC 3 cut(s) 1570, 1831, 2612
Hin6I GCGC 3 cut(s) 1568, 1829, 2610
HinP1I GCGC 3 cut(s) 1568, 1829, 2610
HincII GTYRAC 3 cut(s) 156, 598, 2304
HindII GTYRAC 3 cut(s) 156, 598, 2304
HphI GGTGA 9 cut(s) 163, 331, 541, 838, 1276, 1662, 2218, 2432, 2534
Hpy166II GTNNAC 9 cut(s) 156, 598, 651, 1318, 1408, 1931, 2191, 2209, 2304
Hpy8I GTNNAC 9 cut(s) 156, 598, 651, 1318, 1408, 1931, 2191, 2209, 2304
Hpy99I CGWCG 3 cut(s) 170, 371, 2180
HpyCH4III ACNGT 5 cut(s) 273, 1117, 1315, 1762, 2227
HpyCH4IV ACGT 7 cut(s) 24, 339, 720, 1720, 2193, 2253, 2601
HpyF10VI GCNNNNNNNGC 4 cut(s) 11, 461, 665, 2387
HpyF3I CTNAG 6 cut(s) 603, 664, 1022, 1508, 1837, 2507
HpySE526I ACGT 7 cut(s) 24, 339, 720, 1720, 2193, 2253, 2601
HspAI GCGC 3 cut(s) 1568, 1829, 2610
Kpn2I TCCGGA 2 cut(s) 802, 863
KpnI GGTACC 1 cut(s) 516
KroI GCCGGC 1 cut(s) 1324
KroNI GCCGGC 1 cut(s) 1326
Ksp22I TGATCA 3 cut(s) 1434, 2461, 2524
LmnI GCTCC 1 cut(s) 2032
Lsp1109I GCAGC 3 cut(s) 1663, 2053, 2547
LweI GCATC 9 cut(s) 23, 868, 1104, 1131, 1369, 1529, 2053, 2399, 2551
MaeI CTAG 5 cut(s) 683, 732, 1166, 1238, 1850
MaeII ACGT 7 cut(s) 24, 339, 720, 1720, 2193, 2253, 2601
MaeIII GTNAC 7 cut(s) 25, 169, 547, 871, 949, 1306, 2591
MfeI CAATTG 2 cut(s) 1146, 2184
MflI RGATCY 1 cut(s) 1469
MhlI GDGCHC 7 cut(s) 588, 609, 661, 1509, 2336, 2454, 2584
MlsI TGGCCA 1 cut(s) 674
MluNI TGGCCA 1 cut(s) 674
MlyI GAGTC 5 cut(s) 151, 582, 689, 2475, 2557
MmeI TCCRAC 4 cut(s) 793, 1033, 1822, 2397
Mox20I TGGCCA 1 cut(s) 674
Mph1103I ATGCAT 2 cut(s) 1963, 2544
MroI TCCGGA 2 cut(s) 802, 863
MroNI GCCGGC 1 cut(s) 1324
MroXI GAANNNNTTC 1 cut(s) 892
MscI TGGCCA 1 cut(s) 674
MslI CAYNNNNRTG 2 cut(s) 1960, 2547
Msp20I TGGCCA 1 cut(s) 674
MspA1I CMGCKG 1 cut(s) 2516
MspR9I CCNGG 6 cut(s) 560, 850, 1790, 1983, 2016, 2336
MunI CAATTG 2 cut(s) 1146, 2184
MvaI CCWGG 2 cut(s) 1983, 2016
MwoI GCNNNNNNNGC 4 cut(s) 11, 461, 665, 2387
NaeI GCCGGC 1 cut(s) 1326
NciI CCSGG 4 cut(s) 560, 850, 1790, 2336
NcoI CCATGG 1 cut(s) 330
NdeI CATATG 2 cut(s) 1957, 2169
NgoMIV GCCGGC 1 cut(s) 1324
NlaIV GGNNCC 4 cut(s) 463, 514, 1064, 2034
NmeAIII GCCGAG 4 cut(s) 588, 607, 608, 1408
NmuCI GTSAC 3 cut(s) 25, 169, 547
NsiI ATGCAT 2 cut(s) 1963, 2544
NspI RCATGY 2 cut(s) 1634, 2542
NspV TTCGAA 1 cut(s) 1798
PaeI GCATGC 1 cut(s) 2542
PaeR7I CTCGAG 1 cut(s) 570
PagI TCATGA 3 cut(s) 855, 2257, 2458
PceI AGGCCT 1 cut(s) 1250
PcsI WCGNNNNNNNCGW 2 cut(s) 1620, 2109
PdiI GCCGGC 1 cut(s) 1326
PdmI GAANNNNTTC 1 cut(s) 892
PfeI GAWTC 5 cut(s) 812, 867, 1158, 1290, 2180
PfoI TCCNGGA 1 cut(s) 1981
PinAI ACCGGT 1 cut(s) 2497
PkrI GCNGC 4 cut(s) 466, 1653, 2068, 2537
PleI GAGTC 5 cut(s) 151, 581, 689, 2475, 2557
PpsI GAGTC 5 cut(s) 151, 581, 689, 2475, 2557
Ppu21I YACGTR 1 cut(s) 2194
PshAI GACNNNNGTC 1 cut(s) 161
Psp124BI GAGCTC 4 cut(s) 588, 609, 1509, 2454
Psp1406I AACGTT 1 cut(s) 2253
Psp6I CCWGG 2 cut(s) 1981, 2014
PspFI CCCAGC 1 cut(s) 2512
PspGI CCWGG 2 cut(s) 1981, 2014
PspN4I GGNNCC 4 cut(s) 463, 514, 1064, 2034
PspPI GGNCC 7 cut(s) 254, 562, 938, 1063, 1225, 1931, 2018
PspXI VCTCGAGB 1 cut(s) 570
PsrI GAACNNNNNNTAC 2 cut(s) 1018, 1050
PsuI RGATCY 1 cut(s) 1469
PvuII CAGCTG 1 cut(s) 2516
RsaI GTAC 7 cut(s) 514, 652, 1114, 1395, 1491, 1917, 2192
RsaNI GTAC 7 cut(s) 513, 651, 1113, 1394, 1490, 1916, 2191
RseI CAYNNNNRTG 2 cut(s) 1960, 2547
SacI GAGCTC 4 cut(s) 588, 609, 1509, 2454
SatI GCNGC 4 cut(s) 465, 1652, 2067, 2536
Sau96I GGNCC 7 cut(s) 254, 562, 938, 1063, 1225, 1931, 2018
ScaI AGTACT 1 cut(s) 1395
SchI GAGTC 5 cut(s) 151, 582, 689, 2475, 2557
ScrFI CCNGG 6 cut(s) 560, 850, 1790, 1983, 2016, 2336
SduI GDGCHC 7 cut(s) 588, 609, 661, 1509, 2336, 2454, 2584
SfaNI GCATC 9 cut(s) 23, 868, 1104, 1131, 1369, 1529, 2053, 2399, 2551
Sfr274I CTCGAG 1 cut(s) 570
SfuI TTCGAA 1 cut(s) 1798
SinI GGWCC 5 cut(s) 254, 562, 938, 1225, 1931
SlaI CTCGAG 1 cut(s) 570
SmiMI CAYNNNNRTG 2 cut(s) 1960, 2547
SmlI CTYRAG 2 cut(s) 570, 587
SmoI CTYRAG 2 cut(s) 570, 587
SphI GCATGC 1 cut(s) 2542
SseBI AGGCCT 1 cut(s) 1250
SsiI CCGC 8 cut(s) 287, 397, 465, 552, 686, 1017, 2030, 2231
SspI AATATT 3 cut(s) 34, 297, 1033
SspMI CTAG 5 cut(s) 683, 732, 1166, 1238, 1850
SstI GAGCTC 4 cut(s) 588, 609, 1509, 2454
StuI AGGCCT 1 cut(s) 1250
StyD4I CCNGG 6 cut(s) 558, 848, 1788, 1981, 2014, 2334
StyI CCWWGG 4 cut(s) 330, 508, 529, 1045
TaaI ACNGT 5 cut(s) 273, 1117, 1315, 1762, 2227
TaiI ACGT 7 cut(s) 27, 342, 723, 1723, 2196, 2256, 2604
TaqI TCGA 9 cut(s) 324, 571, 960, 1197, 1614, 1798, 1854, 2103, 2454
TatI WGTACW 3 cut(s) 650, 1393, 1489
TauI GCSGC 1 cut(s) 467
TfiI GAWTC 5 cut(s) 812, 867, 1158, 1290, 2180
TscAI CASTG 3 cut(s) 607, 826, 1516
TseFI GTSAC 3 cut(s) 25, 169, 547
TseI GCWGC 3 cut(s) 1651, 2066, 2535
Tsp45I GTSAC 3 cut(s) 25, 169, 547
TspGWI ACGGA 3 cut(s) 886, 2123, 2333
TspRI CASTG 3 cut(s) 607, 826, 1516
VpaK11BI GGWCC 5 cut(s) 254, 562, 938, 1225, 1931
XapI RAATTY 1 cut(s) 1845
XbaI TCTAGA 1 cut(s) 1237
XceI RCATGY 2 cut(s) 1634, 2542
XhoI CTCGAG 1 cut(s) 570
XmnI GAANNNNTTC 1 cut(s) 892
XspI CTAG 5 cut(s) 683, 732, 1166, 1238, 1850
ZrmI AGTACT 1 cut(s) 1395
Zsp2I ATGCAT 2 cut(s) 1963, 2544
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.