Prupe.4G271900_v2.0.a1

Cation H( ) antiporter

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
prunus_persica
Pp04
OG0017951
Physical Location & Seq
Reverse (-)
22301213 .. 22304416
3204 bp
Loading structure...
UTR
Exon/CDS
Intron
Prupe.4G271900.1

Sequence Viewer

Length: 2577 bp
ATGGCAGCAGCAGTAGCTGCGGTGAAAGCTGCGGCGAATAATTCGTCAGATGGTTCAATAGTATGTTATGCGCCAACCATGATCACCACAAACGGTATATGGCAGGGTGACAATCCTTTGGACTACTCCCTCCCGCTCTTCATTTTGCAGTTAACTATGGTTGTCGTTACTACTCGTATCCTCGTTATCATTCTCAAACCCTTTCGTCAACCTCGTGTCATCTCAGAAATTTTGGGTGGAGTATTATTGGGGCCATCGGTGCTTGGAAAGAGTGATAAATTCGCCACCACAATATTCCCTCTAAGAAGTGTGATGGTCTTGGAGACAATGGCAAATGTGGGTCTCCTCTACTTCCTCTTCCTTGTTGGATTGGAGATGGATATTTCCGTAATTCGGCGCATGGGCAAGAGGGCCATCGTCATTGCCATTGCCGGCATGATCCTCCCCTTTCTCATCGGCGCCGCCTTCTCCTTCCTCCTCCACAAGCGAGAGCAACCCATGAACCAAGGGACTTTCATTCTCTTCCTCGGCGTCGCCCTCTCCGTCACCGCCTTCCCTGTACTCGCCCGTATACTAGCCGAGCTCAAGCTCGTCAACACCGAGCTAGGCAGGATCGCCCTCTCCTCCGCCCTCGTCAACGACATGTGCGCCTGGGCCCTCTTAGCCTTGGCCATTGCCCTTGCGGAAAACGACACCACCTCGTTGGCTTCCCTTTATGTCGTCCTCTCCAGCGCAGCCTTTGTCCTCATCTGCATCTTCATCGTCCGCCCTGCCGTGTGCTGGATCATCCGAAGAACCCCCGAGGGAGAGAGCTTCAGCGACTTCTACATCTGCCTCATCCTCACCGGTGTCATGATCTCGGGCTTCATTACCGACGCCATCGGGACCCACTCCGTTTTCGGGGCCTTCGTGTTCGGTTTAGTCATCCCCAACGGGCCTCTGGGCGCCACCCTGATCGAGAAGCTGGAGGACTTTGTCTCGGGGCTTTTGCTTCCTCTTTTCTTCGCAATTAGCGGTCTCAGAACCAACGTAGGCAGCATCCAGGGACCTGGCACGTGGGGGATTTTGTTACTGGTCATATTTCTGGCATGTGTGGGGAAAATTGCAGGCACTGTCATCGTCTGCCTCTTCTACCAGATGCCCTTCGACGAAGGCTTCACTCTAGGCCTGCTCATGAACACCAAAGGCCTGGTCGAGCTCATTGTGCTCAATGTCGGAAGGGACCAGAAAGTTTTGGACGACGAGGCGTTTGCCATTATGGTGATTGTGGCGGTCGTGATGACCGGAATCATCACCCCCATCGTCACCACCGTTTACAAGCCGGCCAGAAGGTTTATACCTTACAAACGACGTAGCATTCAGAGATCCAAGCCTGACGCGGAGTTGCGAGTACTTGTCTGCATCCACACCCCTCGAAATGTGCCCACTATGATCAATCTCCTTGAAGCTTCCCACCCCACTAAAAGATCCCCGCTCTGCATCTACGTCCTCCACCTGGTGGAGTTGAGCGGGCGGGCTTCCGCCATGCTCATTGTCCACAACACTAGAAAATCCGGCAGACCTGCGCTCAACAGAACTCAGGCCCAGTCGGATCACATCATCAACGCTTTTGAGAACTATGAGCAGCACGCCGGCTGCGTCTCCGTGCAGCCTCTCACAGCCATCTCTCCCTACTCCACAATGCACGAGGACATCTGCAACTTGGCGGAGGACAAGCGCGTCGCCTTCTTAATCATTCCTTTTCACAAGCAGCAAACAGTGGACGGAGGGATGGAGGCCATGAACCCCGCGTTTCGAACAATGAACCAGAACGTTCTTGCAAACGCTCCTTGCTCAGTTGGGATTCTCGTTGACCGAGGACTCAACGGCTCCAACCGCTTGGCTGCCAACCAAGTGTCTCATCACGTGGTCGTTCTCTTCTTCGGGGGACCCGATGATAGAGAGGCGCTGTCCTACGCGTGGAGGATGTCAGAGCATCCGGGGATAAGCCTGACCGTGATGCGGTTTGTTCCTGGGGATGGCGCTGAAGGAGCGAAAAGCGCTTCTGCTTCTGCTGCTGCTTCCGCCACTACCGCTACTGATGCGGAGGGGAACAATTCAAGGATACTGTCGGTGGAGACGGACTTAGGGTGGGAGAAGCAACTGGACGAGGAGTTGATCAATGATTTTAGAATGAAGAATGCCAACGACGAGTCCATTCTTTACACTGAGAAGTTTGTGAATAACGGAGAGGAGACGGTGGCGGCGATTAGGTCAATGGACAACGTTCATGACCTGTTTATTGTAGGGAGAGGGCAAGGCATGATATCGCCTTTAACGGCGGGACTGACGGACTGGAGTGAGTGCCCGGAACTTGGGGCCATTGGGGATTTGTTAGCTTCTTCAGATTTTGCAGCGACGGCATCAGTTTTGGTGATGCACCAATATGTTGGGGATCAAGATCAAGGCCCTGCGGAAGACGTAGACACCCTTCAAACTCCAAACAGCCCAGACGAACAAAACGCACAGTTTAACAATGTCGACCAGATCAACCGCAGGTCTACACCACCCAGGCCCCAAGATGGTTTTAACCCCTAA

Protein Analysis

859

Amino Acids

92.95

Weight (kDa)

5.55

Isoelectric Point (pI)

35.96

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0017951)

Species Orthologous Gene IDs
arabidopsis_thaliana AT2G13620
fragaria_vesca FvH4_1g24460
malus_domestica MD01G1005100.v1.1
prunus_persica Prupe.4G271900_v2.0.a1
rosa_chinensis RchiOBHm_Chr2g0121601
rosa_laevigata RLG00000018626
rosa_roxburghii Rroxscaffold_2G00122330
rosa_rugosa Rorug02G0233800
rosa_samantha Rh2BG301500 Rh2CG280300
rosa_wichuraiana Rw2G023310

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 1718
Acc36I ACCTGC 2 cut(s) 1570, 2526
AccB1I GGYRCC 2 cut(s) 458, 944
AccB7I CCANNNNNTGG 1 cut(s) 1498
AccBSI CCGCTC 3 cut(s) 136, 1474, 1509
AccI GTMKAC 4 cut(s) 571, 2463, 2520, 2540
AccII CGCG 4 cut(s) 1379, 1719, 1790, 1958
AclI AACGTT 2 cut(s) 1812, 2265
AclWI GGATC 7 cut(s) 433, 620, 791, 1359, 1461, 1599, 2442
AcoI YGGCCR 2 cut(s) 669, 1323
AcsI RAATTY 2 cut(s) 228, 278
AcuI CTGAAG 3 cut(s) 799, 2046, 2367
AcvI CACGTG 2 cut(s) 1056, 1906
AcyI GRCGYC 4 cut(s) 459, 531, 876, 945
AdeI CACNNNGTG 1 cut(s) 1498
AfaI GTAC 2 cut(s) 561, 1392
AfeI AGCGCT 1 cut(s) 2041
AflIII ACRYGT 2 cut(s) 642, 1956
AgeI ACCGGT 1 cut(s) 845
AgsI TTSAA 4 cut(s) 57, 1445, 2100, 2474
AjnI CCWGG 7 cut(s) 650, 1041, 1048, 1188, 1494, 2011, 2549
Alw21I GWGCWC 3 cut(s) 585, 1200, 1209
Alw26I GTCTC 8 cut(s) 317, 347, 982, 1022, 1645, 1902, 2111, 2228
AlwI GGATC 7 cut(s) 433, 620, 791, 1359, 1461, 1599, 2442
AlwNI CAGNNNCTG 2 cut(s) 17, 1112
Ama87I CYCGRG 3 cut(s) 800, 859, 979
Aor51HI AGCGCT 1 cut(s) 2041
ApaI GGGCCC 1 cut(s) 658
ApoI RAATTY 2 cut(s) 228, 278
ArsI GACNNNNNNTTYG 6 cut(s) 29, 61, 1176, 1208, 1232, 1264
AsiGI ACCGGT 1 cut(s) 845
AsuC2I CCSGG 2 cut(s) 1980, 2348
AsuHPI GGTGA 9 cut(s) 34, 76, 119, 538, 835, 1273, 1285, 1297, 2425
AsuII TTCGAA 1 cut(s) 1795
AvaI CYCGRG 3 cut(s) 800, 859, 979
AvaII GGWCC 4 cut(s) 885, 1046, 1222, 1928
BaeGI GKGCMC 3 cut(s) 658, 1425, 2348
BalI TGGCCA 1 cut(s) 671
BanI GGYRCC 2 cut(s) 458, 944
BanII GRGCYC 3 cut(s) 585, 658, 1200
BarI GAAGNNNNNNTAC 2 cut(s) 2454, 2486
BauI CACGAG 2 cut(s) 213, 1685
BbrPI CACGTG 2 cut(s) 1056, 1906
BbsI GAAGAC 1 cut(s) 2463
Bbv12I GWGCWC 3 cut(s) 585, 1200, 1209
BceAI ACGGC 4 cut(s) 758, 1882, 2334, 2415
BcgI CGANNNNNNTGC 6 cut(s) 742, 776, 1099, 1133, 1232, 1266
BciT130I CCWGG 7 cut(s) 652, 1043, 1050, 1190, 1496, 2013, 2551
BciVI GTATCC 2 cut(s) 188, 2097
BclI TGATCA 3 cut(s) 81, 1431, 2157
BcnI CCSGG 2 cut(s) 1980, 2348
BcoDI GTCTC 8 cut(s) 317, 347, 982, 1022, 1645, 1902, 2111, 2228
BfaI CTAG 4 cut(s) 575, 605, 1163, 1545
BfoI RGCGCY 5 cut(s) 462, 948, 1949, 2025, 2043
BfuAI ACCTGC 2 cut(s) 1570, 2526
BfuI GTATCC 2 cut(s) 188, 2097
BmcAI AGTACT 1 cut(s) 1392
Bme1390I CCNGG 9 cut(s) 652, 1043, 1050, 1190, 1496, 1980, 2013, 2348, 2551
Bme18I GGWCC 4 cut(s) 885, 1046, 1222, 1928
BmeT110I CYCGRG 3 cut(s) 800, 859, 979
BmrFI CCNGG 9 cut(s) 652, 1043, 1050, 1190, 1496, 1980, 2013, 2348, 2551
BmrI ACTGGG 1 cut(s) 1579
BmuI ACTGGG 1 cut(s) 1579
BpiI GAAGAC 1 cut(s) 2463
BpmI CTGGAG 3 cut(s) 712, 986, 2356
Bpu14I TTCGAA 1 cut(s) 1795
BpuEI CTTGAG 1 cut(s) 569
BpuMI CCSGG 2 cut(s) 1980, 2348
BsaAI YACGTR 2 cut(s) 1056, 1906
BsaBI GATNNNNATC 1 cut(s) 2439
BsaHI GRCGYC 4 cut(s) 459, 531, 876, 945
BsaI GGTCTC 2 cut(s) 347, 1022
BsaWI WCCGGW 2 cut(s) 845, 1283
Bse118I RCCGGY 4 cut(s) 431, 845, 1321, 1631
Bse1I ACTGG 4 cut(s) 1077, 1585, 2148, 2339
Bse3DI GCAATG 3 cut(s) 420, 426, 672
Bse8I GATNNNNATC 1 cut(s) 2439
BseBI CCWGG 7 cut(s) 652, 1043, 1050, 1190, 1496, 2013, 2551
BseGI GGATG 9 cut(s) 786, 837, 924, 1038, 1401, 1776, 1971, 1975, 2023
BseJI GATNNNNATC 1 cut(s) 2439
BseMI GCAATG 3 cut(s) 420, 426, 672
BseMII CTCAG 5 cut(s) 237, 1033, 1592, 1848, 2199
BseNI ACTGG 4 cut(s) 1077, 1585, 2148, 2339
BseRI GAGGAG 5 cut(s) 335, 467, 613, 2165, 2246
BseSI GKGCMC 3 cut(s) 658, 1425, 2348
BsgI GTGCAG 1 cut(s) 1667
Bsh1236I CGCG 4 cut(s) 1379, 1719, 1790, 1958
Bsh1285I CGRYCG 1 cut(s) 1275
BshNI GGYRCC 2 cut(s) 458, 944
BshTI ACCGGT 1 cut(s) 845
BsiEI CGRYCG 1 cut(s) 1275
BsiHKAI GWGCWC 3 cut(s) 585, 1200, 1209
BsiHKCI CYCGRG 3 cut(s) 800, 859, 979
BsiSI CCGG 8 cut(s) 432, 846, 1284, 1322, 1554, 1632, 1979, 2348
BslFI GGGAC 6 cut(s) 523, 898, 1059, 1235, 1941, 2337
BsmAI GTCTC 8 cut(s) 317, 347, 982, 1022, 1645, 1902, 2111, 2228
BsmBI CGTCTC 3 cut(s) 1645, 2111, 2228
BsmFI GGGAC 6 cut(s) 523, 898, 1059, 1235, 1941, 2337
BsmI GAATGC 2 cut(s) 1356, 2185
Bso31I GGTCTC 2 cut(s) 347, 1022
BsoBI CYCGRG 3 cut(s) 800, 859, 979
Bsp119I TTCGAA 1 cut(s) 1795
Bsp120I GGGCCC 1 cut(s) 654
Bsp1286I GDGCHC 6 cut(s) 585, 658, 1200, 1209, 1425, 2348
BspCNI CTCAG 5 cut(s) 236, 1032, 1591, 1847, 2200
BspFNI CGCG 4 cut(s) 1379, 1719, 1790, 1958
BspHI TCATGA 3 cut(s) 852, 1173, 2269
BspMI ACCTGC 2 cut(s) 1570, 2526
BspPI GGATC 7 cut(s) 433, 620, 791, 1359, 1461, 1599, 2442
BspQI GCTCTTC 1 cut(s) 143
BspT104I TTCGAA 1 cut(s) 1795
BspT107I GGYRCC 2 cut(s) 458, 944
BspTNI GGTCTC 2 cut(s) 347, 1022
BsrBI CCGCTC 3 cut(s) 136, 1474, 1509
BsrDI GCAATG 3 cut(s) 420, 426, 672
BsrFI RCCGGY 4 cut(s) 431, 845, 1321, 1631
BsrI ACTGG 4 cut(s) 1077, 1585, 2148, 2339
BssAI RCCGGY 4 cut(s) 431, 845, 1321, 1631
BssNAI GTATAC 1 cut(s) 572
BssNI GRCGYC 4 cut(s) 459, 531, 876, 945
BssSI CACGAG 2 cut(s) 213, 1685
BssT1I CCWWGG 2 cut(s) 505, 666
Bst1107I GTATAC 1 cut(s) 572
Bst2BI CACGAG 2 cut(s) 213, 1685
Bst2UI CCWGG 7 cut(s) 652, 1043, 1050, 1190, 1496, 2013, 2551
Bst4CI ACNGT 8 cut(s) 95, 1114, 1312, 1759, 1996, 2109, 2239, 2508
Bst6I CTCTTC 5 cut(s) 143, 362, 527, 1133, 1922
BstACI GRCGYC 4 cut(s) 459, 531, 876, 945
BstAPI GCANNNNNTGC 1 cut(s) 17
BstBAI YACGTR 2 cut(s) 1056, 1906
BstBI TTCGAA 1 cut(s) 1795
BstC8I GCNNGC 8 cut(s) 433, 1108, 1169, 1323, 1511, 1515, 1629, 1633
BstDEI CTNAG 8 cut(s) 223, 302, 661, 1019, 1578, 1834, 2125, 2208
BstF5I GGATG 9 cut(s) 786, 837, 924, 1038, 1401, 1776, 1971, 1975, 2023
BstFNI CGCG 4 cut(s) 1379, 1719, 1790, 1958
BstH2I RGCGCY 5 cut(s) 462, 948, 1949, 2025, 2043
BstMAI GTCTC 8 cut(s) 317, 347, 982, 1022, 1645, 1902, 2111, 2228
BstMCI CGRYCG 1 cut(s) 1275
BstNI CCWGG 7 cut(s) 652, 1043, 1050, 1190, 1496, 2013, 2551
BstNSI RCATGY 2 cut(s) 646, 1092
BstSCI CCNGG 9 cut(s) 650, 1041, 1048, 1188, 1494, 1978, 2011, 2346, 2549
BstSLI GKGCMC 3 cut(s) 658, 1425, 2348
BstUI CGCG 4 cut(s) 1379, 1719, 1790, 1958
BstV2I GAAGAC 1 cut(s) 2463
BstX2I RGATCY 2 cut(s) 1364, 1466
BstXI CCANNNNNNTGG 5 cut(s) 703, 1049, 1189, 1879, 2429
BstYI RGATCY 2 cut(s) 1364, 1466
BstZ17I GTATAC 1 cut(s) 572
BsuI GTATCC 2 cut(s) 188, 2097
BtsCI GGATG 9 cut(s) 786, 837, 924, 1038, 1401, 1776, 1971, 1975, 2023
BtsIMutI CAGTG 3 cut(s) 1110, 1764, 2205
BveI ACCTGC 2 cut(s) 1570, 2526
Cac8I GCNNGC 8 cut(s) 433, 1108, 1169, 1323, 1511, 1515, 1629, 1633
CaiI CAGNNNCTG 2 cut(s) 17, 1112
CciI TCATGA 3 cut(s) 852, 1173, 2269
Cfr10I RCCGGY 4 cut(s) 431, 845, 1321, 1631
CseI GACGC 5 cut(s) 520, 884, 1385, 1627, 1708
CsiI ACCWGGT 1 cut(s) 1494
Csp6I GTAC 2 cut(s) 560, 1391
CspAI ACCGGT 1 cut(s) 845
CviQI GTAC 2 cut(s) 560, 1391
DdeI CTNAG 8 cut(s) 223, 302, 661, 1019, 1578, 1834, 2125, 2208
DinI GGCGCC 2 cut(s) 460, 946
DraIII CACNNNGTG 1 cut(s) 1498
DrdI GACNNNNNNGTC 1 cut(s) 1718
DseDI GACNNNNNNGTC 1 cut(s) 1718
EaeI YGGCCR 2 cut(s) 669, 1323
Eam1104I CTCTTC 5 cut(s) 143, 362, 527, 1133, 1922
EarI CTCTTC 5 cut(s) 143, 362, 527, 1133, 1922
EciI GGCGGA 5 cut(s) 616, 755, 1510, 1721, 2053
Ecl136II GAGCTC 2 cut(s) 583, 1198
Eco130I CCWWGG 2 cut(s) 505, 666
Eco147I AGGCCT 2 cut(s) 1167, 1188
Eco24I GRGCYC 3 cut(s) 585, 658, 1200
Eco31I GGTCTC 2 cut(s) 347, 1022
Eco32I GATATC 1 cut(s) 2307
Eco47I GGWCC 4 cut(s) 885, 1046, 1222, 1928
Eco47III AGCGCT 1 cut(s) 2041
Eco53kI GAGCTC 2 cut(s) 583, 1198
Eco57I CTGAAG 3 cut(s) 799, 2046, 2367
Eco72I CACGTG 2 cut(s) 1056, 1906
Eco88I CYCGRG 3 cut(s) 800, 859, 979
EcoICRI GAGCTC 2 cut(s) 583, 1198
EcoO109I RGGNCCY 7 cut(s) 655, 885, 903, 1046, 1928, 2447, 2553
EcoRII CCWGG 7 cut(s) 650, 1041, 1048, 1188, 1494, 2011, 2549
EcoRV GATATC 1 cut(s) 2307
EcoT14I CCWWGG 2 cut(s) 505, 666
EcoT38I GRGCYC 3 cut(s) 585, 658, 1200
EgeI GGCGCC 2 cut(s) 460, 946
EheI GGCGCC 2 cut(s) 460, 946
ErhI CCWWGG 2 cut(s) 505, 666
Esp3I CGTCTC 3 cut(s) 1645, 2111, 2228
FaqI GGGAC 6 cut(s) 523, 898, 1059, 1235, 1941, 2337
FauI CCCGC 6 cut(s) 141, 1479, 1502, 1506, 1795, 2314
FbaI TGATCA 3 cut(s) 81, 1431, 2157
FblI GTMKAC 4 cut(s) 571, 2463, 2520, 2540
FokI GGATG 9 cut(s) 773, 824, 911, 1025, 1388, 1783, 1962, 1978, 2030
FriOI GRGCYC 3 cut(s) 585, 658, 1200
FspBI CTAG 4 cut(s) 575, 605, 1163, 1545
GsuI CTGGAG 3 cut(s) 712, 986, 2356
HaeII RGCGCY 5 cut(s) 462, 948, 1949, 2025, 2043
HapII CCGG 8 cut(s) 432, 846, 1284, 1322, 1554, 1632, 1979, 2348
HgaI GACGC 5 cut(s) 520, 884, 1385, 1627, 1708
Hin1I GRCGYC 4 cut(s) 459, 531, 876, 945
HincII GTYRAC 6 cut(s) 153, 209, 595, 637, 1852, 2521
HindII GTYRAC 6 cut(s) 153, 209, 595, 637, 1852, 2521
HindIII AAGCTT 1 cut(s) 1446
HinfI GANTC 4 cut(s) 1287, 1843, 1860, 2192
HpaI GTTAAC 1 cut(s) 153
HpaII CCGG 8 cut(s) 432, 846, 1284, 1322, 1554, 1632, 1979, 2348
HphI GGTGA 9 cut(s) 34, 76, 119, 538, 835, 1273, 1285, 1297, 2425
Hpy188I TCNGA 9 cut(s) 49, 226, 791, 1022, 1217, 1362, 1591, 1972, 2386
Hpy188III TCNNGA 7 cut(s) 853, 883, 958, 1174, 1276, 2270, 2438
Hpy99I CGWCG 8 cut(s) 536, 878, 1151, 1244, 1353, 1724, 2192, 2401
HpyCH4III ACNGT 8 cut(s) 95, 1114, 1312, 1759, 1996, 2109, 2239, 2508
HpyCH4IV ACGT 8 cut(s) 1029, 1055, 1351, 1485, 1812, 1905, 2265, 2460
HpyF3I CTNAG 8 cut(s) 223, 302, 661, 1019, 1578, 1834, 2125, 2208
HpySE526I ACGT 8 cut(s) 1029, 1055, 1351, 1485, 1812, 1905, 2265, 2460
Hsp92I GRCGYC 4 cut(s) 459, 531, 876, 945
KasI GGCGCC 2 cut(s) 458, 944
KflI GGGWCCC 2 cut(s) 885, 1928
KroI GCCGGC 3 cut(s) 431, 1321, 1631
KroNI GCCGGC 3 cut(s) 433, 1323, 1633
Ksp22I TGATCA 3 cut(s) 81, 1431, 2157
KspAI GTTAAC 1 cut(s) 153
LguI GCTCTTC 1 cut(s) 143
LmnI GCTCC 3 cut(s) 1831, 1874, 2030
MabI ACCWGGT 1 cut(s) 1494
MaeI CTAG 4 cut(s) 575, 605, 1163, 1545
MaeII ACGT 8 cut(s) 1029, 1055, 1351, 1485, 1812, 1905, 2265, 2460
MaeIII GTNAC 5 cut(s) 107, 166, 544, 1068, 1303
MbiI CCGCTC 3 cut(s) 136, 1474, 1509
MflI RGATCY 2 cut(s) 1364, 1466
MhlI GDGCHC 6 cut(s) 585, 658, 1200, 1209, 1425, 2348
MlsI TGGCCA 1 cut(s) 671
MluCI AATT 7 cut(s) 40, 228, 278, 390, 1008, 1101, 2095
MluI ACGCGT 1 cut(s) 1956
MluNI TGGCCA 1 cut(s) 671
Mly113I GGCGCC 2 cut(s) 459, 945
MlyI GAGTC 2 cut(s) 1854, 2201
MmeI TCCRAC 4 cut(s) 346, 1195, 1569, 1896
Mox20I TGGCCA 1 cut(s) 671
MroNI GCCGGC 3 cut(s) 431, 1321, 1631
MscI TGGCCA 1 cut(s) 671
MseI TTAA 5 cut(s) 152, 1730, 2315, 2511, 2568
MslI CAYNNNNRTG 2 cut(s) 1259, 2424
Msp20I TGGCCA 1 cut(s) 671
MspI CCGG 8 cut(s) 432, 846, 1284, 1322, 1554, 1632, 1979, 2348
MspR9I CCNGG 9 cut(s) 652, 1043, 1050, 1190, 1496, 1980, 2013, 2348, 2551
Mva1269I GAATGC 2 cut(s) 1356, 2185
MvaI CCWGG 7 cut(s) 652, 1043, 1050, 1190, 1496, 2013, 2551
MvnI CGCG 4 cut(s) 1379, 1719, 1790, 1958
NaeI GCCGGC 3 cut(s) 433, 1323, 1633
NarI GGCGCC 2 cut(s) 459, 945
NciI CCSGG 2 cut(s) 1980, 2348
NgoMIV GCCGGC 3 cut(s) 431, 1321, 1631
NmeAIII GCCGAG 2 cut(s) 507, 604
NmuCI GTSAC 3 cut(s) 107, 544, 1303
NspI RCATGY 2 cut(s) 646, 1092
NspV TTCGAA 1 cut(s) 1795
PagI TCATGA 3 cut(s) 852, 1173, 2269
PceI AGGCCT 2 cut(s) 1167, 1188
PciI ACATGT 1 cut(s) 642
PciSI GCTCTTC 1 cut(s) 143
PcsI WCGNNNNNNNCGW 8 cut(s) 211, 540, 597, 906, 1308, 1635, 1929, 2243
PctI GAATGC 2 cut(s) 1356, 2185
PdiI GCCGGC 3 cut(s) 433, 1323, 1633
PfeI GAWTC 2 cut(s) 1287, 1843
PflFI GACNNNGTC 1 cut(s) 974
PflMI CCANNNNNTGG 1 cut(s) 1498
PinAI ACCGGT 1 cut(s) 845
PleI GAGTC 2 cut(s) 1854, 2200
PluTI GGCGCC 2 cut(s) 462, 948
PmaCI CACGTG 2 cut(s) 1056, 1906
PmlI CACGTG 2 cut(s) 1056, 1906
PpsI GAGTC 2 cut(s) 1854, 2200
Ppu21I YACGTR 2 cut(s) 1056, 1906
PpuMI RGGWCCY 3 cut(s) 885, 1046, 1928
PscI ACATGT 1 cut(s) 642
Psp124BI GAGCTC 2 cut(s) 585, 1200
Psp1406I AACGTT 2 cut(s) 1812, 2265
Psp5II RGGWCCY 3 cut(s) 885, 1046, 1928
Psp6I CCWGG 7 cut(s) 650, 1041, 1048, 1188, 1494, 2011, 2549
PspCI CACGTG 2 cut(s) 1056, 1906
PspGI CCWGG 7 cut(s) 650, 1041, 1048, 1188, 1494, 2011, 2549
PspOMI GGGCCC 1 cut(s) 654
PspPPI RGGWCCY 3 cut(s) 885, 1046, 1928
PstNI CAGNNNCTG 2 cut(s) 17, 1112
PsuI RGATCY 2 cut(s) 1364, 1466
PsyI GACNNNGTC 1 cut(s) 974
RsaI GTAC 2 cut(s) 561, 1392
RsaNI GTAC 2 cut(s) 560, 1391
RseI CAYNNNNRTG 2 cut(s) 1259, 2424
SacI GAGCTC 2 cut(s) 585, 1200
SalI GTCGAC 1 cut(s) 2519
SapI GCTCTTC 1 cut(s) 143
SaqAI TTAA 5 cut(s) 152, 1730, 2315, 2511, 2568
ScaI AGTACT 1 cut(s) 1392
SchI GAGTC 2 cut(s) 1854, 2201
ScrFI CCNGG 9 cut(s) 652, 1043, 1050, 1190, 1496, 1980, 2013, 2348, 2551
SduI GDGCHC 6 cut(s) 585, 658, 1200, 1209, 1425, 2348
SexAI ACCWGGT 1 cut(s) 1494
SfoI GGCGCC 2 cut(s) 460, 946
SfuI TTCGAA 1 cut(s) 1795
SgrAI CRCCGGYG 1 cut(s) 845
SinI GGWCC 4 cut(s) 885, 1046, 1222, 1928
SmiMI CAYNNNNRTG 2 cut(s) 1259, 2424
SmlI CTYRAG 1 cut(s) 584
SmoI CTYRAG 1 cut(s) 584
Sse9I AATT 7 cut(s) 40, 228, 278, 390, 1008, 1101, 2095
SseBI AGGCCT 2 cut(s) 1167, 1188
SspDI GGCGCC 2 cut(s) 458, 944
SspI AATATT 1 cut(s) 294
SspMI CTAG 4 cut(s) 575, 605, 1163, 1545
SstI GAGCTC 2 cut(s) 585, 1200
StuI AGGCCT 2 cut(s) 1167, 1188
StyD4I CCNGG 9 cut(s) 650, 1041, 1048, 1188, 1494, 1978, 2011, 2346, 2549
StyI CCWWGG 2 cut(s) 505, 666
TaaI ACNGT 8 cut(s) 95, 1114, 1312, 1759, 1996, 2109, 2239, 2508
TaiI ACGT 8 cut(s) 1032, 1058, 1354, 1488, 1815, 1908, 2268, 2463
TaqI TCGA 6 cut(s) 957, 1146, 1194, 1414, 1795, 2520
TaqII GACCGA 1 cut(s) 1869
TasI AATT 7 cut(s) 40, 228, 278, 390, 1008, 1101, 2095
TatI WGTACW 2 cut(s) 559, 1390
TauI GCSGC 3 cut(s) 35, 464, 2246
TfiI GAWTC 2 cut(s) 1287, 1843
Tru1I TTAA 5 cut(s) 152, 1730, 2315, 2511, 2568
Tru9I TTAA 5 cut(s) 152, 1730, 2315, 2511, 2568
TscAI CASTG 3 cut(s) 1117, 1764, 2212
TseFI GTSAC 3 cut(s) 107, 544, 1303
Tsp45I GTSAC 3 cut(s) 107, 544, 1303
TspGWI ACGGA 8 cut(s) 376, 532, 883, 1633, 1779, 2135, 2241, 2345
TspRI CASTG 3 cut(s) 1117, 1764, 2212
Tth111I GACNNNGTC 1 cut(s) 974
Van91I CCANNNNNTGG 1 cut(s) 1498
VpaK11BI GGWCC 4 cut(s) 885, 1046, 1222, 1928
XapI RAATTY 2 cut(s) 228, 278
XceI RCATGY 2 cut(s) 646, 1092
XcmI CCANNNNNNNNNTGG 1 cut(s) 937
XmiI GTMKAC 4 cut(s) 571, 2463, 2520, 2540
XspI CTAG 4 cut(s) 575, 605, 1163, 1545
ZrmI AGTACT 1 cut(s) 1392
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.