Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr01

Orthogroup ID

OG0017951

Physical Location & Seq

Genomic Coordinates Forward (+)

1538971 .. 1541839

Gene Structure 2869 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

MD01G1005100.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 2574 bp

ATGGCGGCGGCAGTGGCTGCAGCAACTGGTGCGACGAATAATTCATCAGATGGTTCAATAGTATGTTACGCTCCAACCATGATCACTACCAACGGTATATGGCAGGGCGACAATCCTTTAGATTACTCCCTCCCGCTCTTCATCTTGCAATTAACAATGGTCGTCGTCACCACTCGAATTCTCGTTCTCATTCTCAAACCCTTTCGCCAACCTCGTGTTATCTCAGAAATCCTGGGTGGAGTATTATTAGGGCCATCGGTGCTTGGAAAGAGTGATAAATTCGCCAACACAATCTTCCCTCTAAGAAGTGTGATGGTGTTGGAGACAATGGCAAACGTGGGTCTCCTCTACTTTCTCTTCCTCGTCGGATTGGAAATGGACATTTCAGTGATTCGTCGAATGGGGAAAAGGGCTGTTGTCATTGCCATTTCCGGTATGATCCTCCCCTTTCTCATCGGCGCCGCCTTCTCCTTCCTTCTCCACAAGCGAGAACAATCCATGAACCAAGGCACTTTCATCCTCTTCCTTGGCGTCGCCCTCTCCGTCACCGCTTTTCCTGTCCTGGCCCGTGTCCTGGCTGAGCTCAAACTCATCAACACCGAGCTTGGCAGGATTGCCCTCTCCTCCGCTCTCGTCAACGACATGTGCGCTTGGGCTCTCTTGGCTATTGCCATTGCCCTGGCCGAAAATGACTCCACTTCCTTGGCTTCCCTCTACGTCGTCCTCTCCAGCGGCGCCTTTGTTCTAGTTTGTATCTTCATCGTCCGCCCTGCCATTTGTTGGCTCATTCGAAGAACCCCCGAAGGAGAGACCTTCAGTGAGTTCTACATCTGCCTCATCCTCACCGGCGTCATGATCTCCGGCTTCGTCACGGATGCTATCGGGACTCACTCCGTCTTCGGTGCATTTGTGTTTGGGCTGGTGATTCCCAATGGGCCTCTTGGAGCTACCCTGATTGAGAAGCTTGAGGATTTTGTCTCGGGGCTTCTGCTTCCTCTTTTCTTCGCAATCAGCGGTCTCAGAACCAATATAACCAGCATTACTGGCCCCGGCACGTGGGGGATTCTGCTGCTCGTCATATTCCTGGCCTGCATCGGCAAAATAGCCGGCACCGTCATCGTCTGCTTGTTTTACCAGATGCCGTTCGACGAAGGGTTTACTCTAGGGCTCCTCATGAACTGCAAAGGCCTCGTCGAGCTCATTGTTTTGAATGTCGGCAAAGACCAAAAAGTTTTAGACGACGAGGCGTTTGCCATCATGGTAATTGTGGCGGTTGTCATGACTGGAATCATTACGCCAATCGTGACCACCGTCTACAAGCCAGCCAGAAGGTTTATACCTTACAAACGAAGAAGCATTCAGAGGTCGAAGCCGGATGCAGAGTTACGAATACTTGTTTGCGTCCACACTCCTCGAAATGTGCCGACTATGATCAATCTCCTTGAAGCTTCCCACCCGACGAAAAGATCCCCGCTTTGTGTCTACATTCTCCACTTGGTGGAGCTGAGCGGCCGCGCTTCCGCCATGCTCATTGTCCACAACACCAGGAAATCCGGGAGACCTGCTCTCAACAGGACACAAGCTCAGTCGGATCACATTATCAACGCATTCGAAAACTACGAGCAGCACGCGCCGGGCAACTGCGTTTCGGTGCAGCCGCTCACGGCCATTTCCCCCTACTCCACCATGCACGAAGACATCTGCAACTTGGCCGAGGACAAGCGCGTCGCCTTCTTAATCATTCCTTTCCACAAACAGCAAACTGTGGACGGGGGAATGGAGGCAATGAACCCAGCTTTTAGGACAATGAACCAGAACGTGCTTACAAATGCACCTTGCTCCGTTGGAATCCTTGTGGACAGAGGCCTCAACGGCTCCAGTCGCTTGGCTGCTGCAAATCAAATCTCGCATCACGTGGTTATTCTCTTCTTTGGAGGACCCGACGACAGAGAGGCCTTGTCCTATGCGTGGAGGATGTCGGAGCATCCCGGGATAAACCTCACCGTCATGCGGTTTGTTCCAGGGGAGGAAGCTGGACTGGAGAGAGCTGCTTCTACTTCCACTAGTAATGTGGACGGCATCGGGAATAATTCGAGGATACTGTCGGTGGAGATGGACATTGGGTGGGAGAAGCAGCTCGACGAGGAGTTAATCAATGATTTTAGAATGAAAAATGCCAACGAAGAGTCGGTTGTTTACACAGAGAAGGTGGTGAATAACGGAGAGGAGACGGTGGCGGCAATTAGGTCCATGGACAACGTTCACGATCTCTTCATTGTTGGGAGAGGGCAAGGGATGGTATCGCCTTTGACGGCGGGACTGACGGACTGGAGTGAGTGCCCGGAACTTGGTGCCATTGGAGATTTGTTAGCATCTTCAGACTTTGCTGCCACGGCGTCGGTGTTGGTGATGCAACAATATGTTGGAGATCATGATCAAGGACCAGCAGAAGTTGACACCCTCGACACGCCCGACACTCCTCCCGACCAACAAAACCCACAATTTAACAGCGTCCACAACATTAACCGCCGCCCACAACCACCCAGCAGGCCCAACGATGGCTTTAAGCCCTAA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0005575 GO:0005623 GO:0006810 GO:0006811 GO:0006812 GO:0006885 GO:0008150 GO:0012505 GO:0015672 GO:0042592 GO:0044464 GO:0048878 GO:0050801 GO:0051179 GO:0051234 GO:0055067 GO:0055080 GO:0065007 GO:0065008 GO:0098771

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

Na_H_Exchanger

Protein Analysis

858

Amino Acids

93.13

Weight (kDa)

5.66

Isoelectric Point (pI)

37.04

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
Na_H_Exchanger	PF00999	59 - 436	3.3e-64	Sodium/hydrogen exchanger, transmembrane
CHX17_2nd	PF23256	491 - 633	8.1e-30	Plant cation/H(+) antiporter domain
CHX17_C	PF23259	638 - 809	1.4e-21	Plant cation/H(+) antiporter, C-terminal domain

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0017951)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT2G13620
fragaria_vesca	FvH4_1g24460
malus_domestica	MD01G1005100.v1.1
prunus_persica	Prupe.4G271900_v2.0.a1
rosa_chinensis	RchiOBHm_Chr2g0121601
rosa_laevigata	RLG00000018626
rosa_roxburghii	Rroxscaffold_2G00122330
rosa_rugosa	Rorug02G0233800
rosa_samantha	Rh2BG301500 Rh2CG280300
rosa_wichuraiana	Rw2G023310

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AasI	GACNNNNNNGTC	1 cut(s)	1724
Acc36I	ACCTGC	1 cut(s)	1570
AccB1I	GGYRCC	4 cut(s)	458, 734, 1109, 2351
AccB7I	CCANNNNNTGG	2 cut(s)	780, 1498
AccBSI	CCGCTC	4 cut(s)	136, 629, 1509, 1660
AccI	GTMKAC	2 cut(s)	1314, 1482
AccII	CGCG	3 cut(s)	1515, 1631, 1725
AclI	AACGTT	1 cut(s)	2259
AclWI	GGATC	3 cut(s)	433, 1461, 1599
AcoI	YGGCCR	4 cut(s)	681, 1510, 1665, 1710
AcsI	RAATTY	2 cut(s)	177, 278
AcuI	CTGAAG	2 cut(s)	799, 2361
AcvI	CACGTG	2 cut(s)	1056, 1915
AcyI	GRCGYC	5 cut(s)	459, 531, 735, 849, 2396
AdeI	CACNNNGTG	1 cut(s)	1498
AfiI	CCNNNNNNNGG	8 cut(s)	574, 780, 1056, 1498, 1968, 2010, 2348, 2558
AflIII	ACRYGT	1 cut(s)	642
AgsI	TTSAA	3 cut(s)	57, 1210, 1445
AhlI	ACTAGT	1 cut(s)	2063
AjnI	CCWGG	7 cut(s)	231, 561, 573, 678, 1083, 1544, 2020
AjuI	GAANNNNNNNTTGG	2 cut(s)	278, 310
Alw21I	GWGCWC	2 cut(s)	585, 1200
Alw26I	GTCTC	7 cut(s)	317, 347, 803, 982, 1022, 1552, 2222
AlwI	GGATC	3 cut(s)	433, 1461, 1599
AlwNI	CAGNNNCTG	2 cut(s)	17, 26
Ama87I	CYCGRG	2 cut(s)	979, 1988
ApoI	RAATTY	2 cut(s)	177, 278
ArsI	GACNNNNNNTTYG	4 cut(s)	1176, 1208, 1232, 1264
AspLEI	GCGC	6 cut(s)	461, 650, 737, 1517, 1633, 1725
AspS9I	GGNCC	8 cut(s)	251, 565, 935, 1046, 1937, 2247, 2441, 2550
AsuC2I	CCSGG	6 cut(s)	1050, 1555, 1635, 1989, 1990, 2342
AsuHPI	GGTGA	7 cut(s)	160, 538, 835, 934, 1993, 2224, 2419
AsuII	TTCGAA	2 cut(s)	790, 1611
AvaI	CYCGRG	2 cut(s)	979, 1988
AvaII	GGWCC	3 cut(s)	1937, 2247, 2441
BaeGI	GKGCMC	1 cut(s)	2342
BanI	GGYRCC	4 cut(s)	458, 734, 1109, 2351
BanII	GRGCYC	4 cut(s)	585, 658, 1170, 1200
BauI	CACGAG	1 cut(s)	213
BbrPI	CACGTG	2 cut(s)	1056, 1915
BbsI	GAAGAC	2 cut(s)	889, 1701
Bbv12I	GWGCWC	2 cut(s)	585, 1200
BccI	CCATC	7 cut(s)	44, 262, 307, 1262, 2107, 2290, 2552
BceAI	ACGGC	6 cut(s)	1126, 1680, 1888, 2092, 2328, 2409
BcgI	CGANNNNNNTGC	6 cut(s)	1099, 1133, 1171, 1205, 1232, 1266
BciT130I	CCWGG	7 cut(s)	233, 563, 575, 680, 1085, 1546, 2022
BciVI	GTATCC	1 cut(s)	2091
BclI	TGATCA	3 cut(s)	81, 1431, 2434
BcnI	CCSGG	6 cut(s)	1050, 1555, 1635, 1989, 1990, 2342
BcoDI	GTCTC	7 cut(s)	317, 347, 803, 982, 1022, 1552, 2222
BcuI	ACTAGT	1 cut(s)	2063
BfaI	CTAG	3 cut(s)	746, 1163, 2064
BfmI	CTRYAG	1 cut(s)	18
BfoI	RGCGCY	2 cut(s)	462, 738
BfuAI	ACCTGC	1 cut(s)	1570
BfuI	GTATCC	1 cut(s)	2091
BglI	GCCNNNNNGGC	1 cut(s)	1872
BlpI	GCTNAGC	2 cut(s)	579, 1505
Bme18I	GGWCC	3 cut(s)	1937, 2247, 2441
BmeT110I	CYCGRG	2 cut(s)	979, 1988
BmgT120I	GGNCC	8 cut(s)	251, 565, 935, 1046, 1937, 2247, 2441, 2550
BmiI	GGNNCC	8 cut(s)	460, 736, 1048, 1111, 1169, 1876, 1939, 2353
BmsI	GCATC	9 cut(s)	865, 1101, 1128, 1366, 1918, 1993, 2088, 2381, 2400
BoxI	GACNNNNGTC	1 cut(s)	1310
BpiI	GAAGAC	2 cut(s)	889, 1701
BplI	GAGNNNNNCTC	2 cut(s)	1549, 1581
BpmI	CTGGAG	4 cut(s)	712, 1861, 2060, 2350
Bpu1102I	GCTNAGC	2 cut(s)	579, 1505
Bpu14I	TTCGAA	2 cut(s)	790, 1611
BpuEI	CTTGAG	1 cut(s)	986
BpuMI	CCSGG	6 cut(s)	1050, 1555, 1635, 1989, 1990, 2342
BsaAI	YACGTR	2 cut(s)	1056, 1915
BsaBI	GATNNNNATC	1 cut(s)	2433
BsaHI	GRCGYC	5 cut(s)	459, 531, 735, 849, 2396
BsaI	GGTCTC	4 cut(s)	347, 803, 1022, 1552
BsaWI	WCCGGW	1 cut(s)	431
Bsc4I	CCNNNNNNNGG	8 cut(s)	574, 780, 1056, 1498, 1968, 2010, 2348, 2558
Bse118I	RCCGGY	2 cut(s)	845, 1106
Bse1I	ACTGG	6 cut(s)	31, 1048, 1288, 1878, 2043, 2333
Bse3DI	GCAATG	3 cut(s)	420, 672, 1791
Bse8I	GATNNNNATC	1 cut(s)	2433
BseBI	CCWGG	7 cut(s)	233, 563, 575, 680, 1085, 1546, 2022
BseGI	GGATG	7 cut(s)	516, 837, 880, 1381, 1980, 1984, 2301
BseJI	GATNNNNATC	1 cut(s)	2433
BseLI	CCNNNNNNNGG	8 cut(s)	574, 780, 1056, 1498, 1968, 2010, 2348, 2558
BseMI	GCAATG	3 cut(s)	420, 672, 1791
BseMII	CTCAG	5 cut(s)	237, 570, 1033, 1496, 1598
BseNI	ACTGG	6 cut(s)	31, 1048, 1288, 1878, 2043, 2333
BseRI	GAGGAG	7 cut(s)	335, 613, 1160, 1401, 2159, 2240, 2469
BseSI	GKGCMC	1 cut(s)	2342
BseX3I	CGGCCG	1 cut(s)	1510
BseYI	CCCAGC	2 cut(s)	1792, 2543
BsgI	GTGCAG	1 cut(s)	1673
Bsh1236I	CGCG	3 cut(s)	1515, 1631, 1725
Bsh1285I	CGRYCG	1 cut(s)	1513
BshNI	GGYRCC	4 cut(s)	458, 734, 1109, 2351
BsiEI	CGRYCG	1 cut(s)	1513
BsiHKAI	GWGCWC	2 cut(s)	585, 1200
BsiHKCI	CYCGRG	2 cut(s)	979, 1988
BslFI	GGGAC	2 cut(s)	898, 2331
BslI	CCNNNNNNNGG	8 cut(s)	574, 780, 1056, 1498, 1968, 2010, 2348, 2558
BsmAI	GTCTC	7 cut(s)	317, 347, 803, 982, 1022, 1552, 2222
BsmBI	CGTCTC	1 cut(s)	2222
BsmFI	GGGAC	2 cut(s)	898, 2331
BsmI	GAATGC	2 cut(s)	1356, 1607
Bso31I	GGTCTC	4 cut(s)	347, 803, 1022, 1552
BsoBI	CYCGRG	2 cut(s)	979, 1988
Bsp119I	TTCGAA	2 cut(s)	790, 1611
Bsp1286I	GDGCHC	5 cut(s)	585, 658, 1170, 1200, 2342
Bsp143I	GATC	9 cut(s)	81, 438, 855, 1431, 1466, 1591, 2266, 2428, 2434
Bsp1720I	GCTNAGC	2 cut(s)	579, 1505
Bsp19I	CCATGG	1 cut(s)	2250
BspCNI	CTCAG	5 cut(s)	236, 571, 1032, 1497, 1597
BspFNI	CGCG	3 cut(s)	1515, 1631, 1725
BspHI	TCATGA	4 cut(s)	852, 1173, 1278, 2431
BspLI	GGNNCC	8 cut(s)	460, 736, 1048, 1111, 1169, 1876, 1939, 2353
BspMAI	CTGCAG	1 cut(s)	22
BspMI	ACCTGC	1 cut(s)	1570
BspPI	GGATC	3 cut(s)	433, 1461, 1599
BspQI	GCTCTTC	1 cut(s)	143
BspT104I	TTCGAA	2 cut(s)	790, 1611
BspT107I	GGYRCC	4 cut(s)	458, 734, 1109, 2351
BspTNI	GGTCTC	4 cut(s)	347, 803, 1022, 1552
BsrBI	CCGCTC	4 cut(s)	136, 629, 1509, 1660
BsrDI	GCAATG	3 cut(s)	420, 672, 1791
BsrFI	RCCGGY	2 cut(s)	845, 1106
BsrI	ACTGG	6 cut(s)	31, 1048, 1288, 1878, 2043, 2333
BssAI	RCCGGY	2 cut(s)	845, 1106
BssMI	GATC	9 cut(s)	81, 438, 855, 1431, 1466, 1591, 2266, 2428, 2434
BssNI	GRCGYC	5 cut(s)	459, 531, 735, 849, 2396
BssSI	CACGAG	1 cut(s)	213
BssT1I	CCWWGG	4 cut(s)	505, 526, 702, 2250
Bst2BI	CACGAG	1 cut(s)	213
Bst2UI	CCWGG	7 cut(s)	233, 563, 575, 680, 1085, 1546, 2022
Bst4CI	ACNGT	7 cut(s)	95, 1114, 1312, 1765, 2005, 2103, 2233
Bst6I	CTCTTC	6 cut(s)	143, 362, 527, 1931, 2178, 2276
BstACI	GRCGYC	5 cut(s)	459, 531, 735, 849, 2396
BstAPI	GCANNNNNTGC	2 cut(s)	17, 29
BstBAI	YACGTR	2 cut(s)	1056, 1915
BstBI	TTCGAA	2 cut(s)	790, 1611
BstC8I	GCNNGC	5 cut(s)	1090, 1108, 1323, 1629, 2549
BstDEI	CTNAG	6 cut(s)	223, 302, 579, 1019, 1505, 1584
BstDSI	CCRYGG	2 cut(s)	2250, 2391
BstF5I	GGATG	7 cut(s)	516, 837, 880, 1381, 1980, 1984, 2301
BstFNI	CGCG	3 cut(s)	1515, 1631, 1725
BstH2I	RGCGCY	2 cut(s)	462, 738
BstHHI	GCGC	6 cut(s)	461, 650, 737, 1517, 1633, 1725
BstKTI	GATC	9 cut(s)	84, 441, 858, 1434, 1469, 1594, 2269, 2431, 2437
BstMAI	GTCTC	7 cut(s)	317, 347, 803, 982, 1022, 1552, 2222
BstMBI	GATC	9 cut(s)	81, 438, 855, 1431, 1466, 1591, 2266, 2428, 2434
BstMCI	CGRYCG	1 cut(s)	1513
BstNI	CCWGG	7 cut(s)	233, 563, 575, 680, 1085, 1546, 2022
BstNSI	RCATGY	1 cut(s)	646
BstPAI	GACNNNNGTC	1 cut(s)	1310
BstSFI	CTRYAG	1 cut(s)	18
BstSLI	GKGCMC	1 cut(s)	2342
BstUI	CGCG	3 cut(s)	1515, 1631, 1725
BstV2I	GAAGAC	2 cut(s)	889, 1701
BstX2I	RGATCY	1 cut(s)	1466
BstXI	CCANNNNNNTGG	3 cut(s)	679, 703, 1885
BstYI	RGATCY	1 cut(s)	1466
BstZI	CGGCCG	1 cut(s)	1510
BsuI	GTATCC	1 cut(s)	2091
BtgI	CCRYGG	2 cut(s)	2250, 2391
BtsCI	GGATG	7 cut(s)	516, 837, 880, 1381, 1980, 1984, 2301
BtsI	GCAGTG	1 cut(s)	18
BtsIMutI	CAGTG	3 cut(s)	18, 393, 823
BveI	ACCTGC	1 cut(s)	1570
Cac8I	GCNNGC	5 cut(s)	1090, 1108, 1323, 1629, 2549
CaiI	CAGNNNCTG	2 cut(s)	17, 26
CciI	TCATGA	4 cut(s)	852, 1173, 1278, 2431
CciNI	GCGGCCGC	1 cut(s)	1510
CfoI	GCGC	6 cut(s)	461, 650, 737, 1517, 1633, 1725
Cfr10I	RCCGGY	2 cut(s)	845, 1106
Cfr13I	GGNCC	8 cut(s)	251, 565, 935, 1046, 1937, 2247, 2441, 2550
Cfr9I	CCCGGG	1 cut(s)	1988
CseI	GACGC	6 cut(s)	520, 838, 1390, 1714, 2385, 2500
DdeI	CTNAG	6 cut(s)	223, 302, 579, 1019, 1505, 1584
DinI	GGCGCC	2 cut(s)	460, 736
DpnI	GATC	9 cut(s)	83, 440, 857, 1433, 1468, 1593, 2268, 2430, 2436
DpnII	GATC	9 cut(s)	81, 438, 855, 1431, 1466, 1591, 2266, 2428, 2434
DraIII	CACNNNGTG	1 cut(s)	1498
DrdI	GACNNNNNNGTC	1 cut(s)	1724
DseDI	GACNNNNNNGTC	1 cut(s)	1724
EaeI	YGGCCR	4 cut(s)	681, 1510, 1665, 1710
EagI	CGGCCG	1 cut(s)	1510
Eam1104I	CTCTTC	6 cut(s)	143, 362, 527, 1931, 2178, 2276
EarI	CTCTTC	6 cut(s)	143, 362, 527, 1931, 2178, 2276
EciI	GGCGGA	2 cut(s)	755, 1510
Ecl136II	GAGCTC	2 cut(s)	583, 1198
EclXI	CGGCCG	1 cut(s)	1510
Eco130I	CCWWGG	4 cut(s)	505, 526, 702, 2250
Eco147I	AGGCCT	3 cut(s)	1188, 1866, 1955
Eco24I	GRGCYC	4 cut(s)	585, 658, 1170, 1200
Eco31I	GGTCTC	4 cut(s)	347, 803, 1022, 1552
Eco47I	GGWCC	3 cut(s)	1937, 2247, 2441
Eco52I	CGGCCG	1 cut(s)	1510
Eco53kI	GAGCTC	2 cut(s)	583, 1198
Eco57I	CTGAAG	2 cut(s)	799, 2361
Eco72I	CACGTG	2 cut(s)	1056, 1915
Eco88I	CYCGRG	2 cut(s)	979, 1988
EcoICRI	GAGCTC	2 cut(s)	583, 1198
EcoO109I	RGGNCCY	1 cut(s)	1937
EcoRI	GAATTC	1 cut(s)	177
EcoRII	CCWGG	7 cut(s)	231, 561, 573, 678, 1083, 1544, 2020
EcoT14I	CCWWGG	4 cut(s)	505, 526, 702, 2250
EcoT38I	GRGCYC	4 cut(s)	585, 658, 1170, 1200
EgeI	GGCGCC	2 cut(s)	460, 736
EheI	GGCGCC	2 cut(s)	460, 736
ErhI	CCWWGG	4 cut(s)	505, 526, 702, 2250
Esp3I	CGTCTC	1 cut(s)	2222
FaqI	GGGAC	2 cut(s)	898, 2331
FauI	CCCGC	3 cut(s)	141, 1479, 2308
FbaI	TGATCA	3 cut(s)	81, 1431, 2434
FblI	GTMKAC	2 cut(s)	1314, 1482
FokI	GGATG	7 cut(s)	503, 824, 887, 1388, 1971, 1987, 2308
FriOI	GRGCYC	4 cut(s)	585, 658, 1170, 1200
FspBI	CTAG	3 cut(s)	746, 1163, 2064
GlaI	GCGC	6 cut(s)	460, 649, 736, 1516, 1632, 1724
GsaI	CCCAGC	2 cut(s)	1796, 2547
GsuI	CTGGAG	4 cut(s)	712, 1861, 2060, 2350
HaeII	RGCGCY	2 cut(s)	462, 738
HgaI	GACGC	6 cut(s)	520, 838, 1390, 1714, 2385, 2500
HhaI	GCGC	6 cut(s)	461, 650, 737, 1517, 1633, 1725
Hin1I	GRCGYC	5 cut(s)	459, 531, 735, 849, 2396
Hin6I	GCGC	6 cut(s)	459, 648, 735, 1515, 1631, 1723
HinP1I	GCGC	6 cut(s)	459, 648, 735, 1515, 1631, 1723
HincII	GTYRAC	2 cut(s)	637, 2455
HindII	GTYRAC	2 cut(s)	637, 2455
HindIII	AAGCTT	2 cut(s)	962, 1446
HinfI	GANTC	8 cut(s)	391, 692, 886, 925, 1063, 1287, 1848, 2186
HphI	GGTGA	7 cut(s)	160, 538, 835, 934, 1993, 2224, 2419
Hpy188I	TCNGA	8 cut(s)	49, 226, 368, 1022, 1362, 1591, 1981, 2380
Hpy188III	TCNNGA	9 cut(s)	853, 883, 1174, 1279, 1303, 2083, 2264, 2432, 2483
HpyAV	CCTTC	9 cut(s)	475, 481, 485, 797, 823, 1145, 1323, 1741, 2200
HpyCH4III	ACNGT	7 cut(s)	95, 1114, 1312, 1765, 2005, 2103, 2233
HpyCH4IV	ACGT	6 cut(s)	336, 717, 1055, 1818, 1914, 2259
HpyF3I	CTNAG	6 cut(s)	223, 302, 579, 1019, 1505, 1584
HpySE526I	ACGT	6 cut(s)	336, 717, 1055, 1818, 1914, 2259
Hsp92I	GRCGYC	5 cut(s)	459, 531, 735, 849, 2396
HspAI	GCGC	6 cut(s)	459, 648, 735, 1515, 1631, 1723
KasI	GGCGCC	2 cut(s)	458, 734
KroI	GCCGGC	1 cut(s)	1106
KroNI	GCCGGC	1 cut(s)	1108
Ksp22I	TGATCA	3 cut(s)	81, 1431, 2434
Kzo9I	GATC	9 cut(s)	81, 438, 855, 1431, 1466, 1591, 2266, 2428, 2434
LguI	GCTCTTC	1 cut(s)	143
LmnI	GCTCC	7 cut(s)	76, 944, 1173, 1501, 1844, 1880, 1981
LweI	GCATC	9 cut(s)	865, 1101, 1128, 1366, 1918, 1993, 2088, 2381, 2400
MaeI	CTAG	3 cut(s)	746, 1163, 2064
MaeII	ACGT	6 cut(s)	336, 717, 1055, 1818, 1914, 2259
MaeIII	GTNAC	6 cut(s)	65, 166, 544, 868, 1303, 1383
MalI	GATC	9 cut(s)	83, 440, 857, 1433, 1468, 1593, 2268, 2430, 2436
MbiI	CCGCTC	4 cut(s)	136, 629, 1509, 1660
MboI	GATC	9 cut(s)	81, 438, 855, 1431, 1466, 1591, 2266, 2428, 2434
MflI	RGATCY	1 cut(s)	1466
MhlI	GDGCHC	5 cut(s)	585, 658, 1170, 1200, 2342
MluCI	AATT	8 cut(s)	40, 149, 177, 278, 1263, 2089, 2241, 2501
Mly113I	GGCGCC	2 cut(s)	459, 735
MlyI	GAGTC	3 cut(s)	686, 880, 2195
MmeI	TCCRAC	7 cut(s)	98, 300, 346, 1569, 1825, 1959, 2404
MroNI	GCCGGC	1 cut(s)	1106
MseI	TTAA	6 cut(s)	152, 1736, 2150, 2505, 2523, 2565
MslI	CAYNNNNRTG	2 cut(s)	386, 2006
MspA1I	CMGCKG	2 cut(s)	732, 1014
Mva1269I	GAATGC	2 cut(s)	1356, 1607
MvaI	CCWGG	7 cut(s)	233, 563, 575, 680, 1085, 1546, 2022
MvnI	CGCG	3 cut(s)	1515, 1631, 1725
NaeI	GCCGGC	1 cut(s)	1108
NarI	GGCGCC	2 cut(s)	459, 735
NciI	CCSGG	6 cut(s)	1050, 1555, 1635, 1989, 1990, 2342
NcoI	CCATGG	1 cut(s)	2250
NdeII	GATC	9 cut(s)	81, 438, 855, 1431, 1466, 1591, 2266, 2428, 2434
NgoMIV	GCCGGC	1 cut(s)	1106
NlaIV	GGNNCC	8 cut(s)	460, 736, 1048, 1111, 1169, 1876, 1939, 2353
NmeAIII	GCCGAG	1 cut(s)	1738
NmuCI	GTSAC	4 cut(s)	166, 544, 868, 1303
NotI	GCGGCCGC	1 cut(s)	1510
NspI	RCATGY	1 cut(s)	646
NspV	TTCGAA	2 cut(s)	790, 1611
PagI	TCATGA	4 cut(s)	852, 1173, 1278, 2431
PceI	AGGCCT	3 cut(s)	1188, 1866, 1955
PciI	ACATGT	1 cut(s)	642
PciSI	GCTCTTC	1 cut(s)	143
PcsI	WCGNNNNNNNCGW	5 cut(s)	211, 540, 1308, 1617, 2469
PctI	GAATGC	2 cut(s)	1356, 1607
PdiI	GCCGGC	1 cut(s)	1108
PfeI	GAWTC	5 cut(s)	391, 925, 1063, 1287, 1848
PflMI	CCANNNNNTGG	2 cut(s)	780, 1498
PfoI	TCCNGGA	1 cut(s)	1553
PleI	GAGTC	3 cut(s)	686, 880, 2194
PluTI	GGCGCC	2 cut(s)	462, 738
PmaCI	CACGTG	2 cut(s)	1056, 1915
PmlI	CACGTG	2 cut(s)	1056, 1915
PpsI	GAGTC	3 cut(s)	686, 880, 2194
Ppu21I	YACGTR	2 cut(s)	1056, 1915
PpuMI	RGGWCCY	1 cut(s)	1937
PscI	ACATGT	1 cut(s)	642
PshAI	GACNNNNGTC	1 cut(s)	1310
Psp124BI	GAGCTC	2 cut(s)	585, 1200
Psp1406I	AACGTT	1 cut(s)	2259
Psp5II	RGGWCCY	1 cut(s)	1937
Psp6I	CCWGG	7 cut(s)	231, 561, 573, 678, 1083, 1544, 2020
PspCI	CACGTG	2 cut(s)	1056, 1915
PspFI	CCCAGC	2 cut(s)	1792, 2543
PspGI	CCWGG	7 cut(s)	231, 561, 573, 678, 1083, 1544, 2020
PspN4I	GGNNCC	8 cut(s)	460, 736, 1048, 1111, 1169, 1876, 1939, 2353
PspPI	GGNCC	8 cut(s)	251, 565, 935, 1046, 1937, 2247, 2441, 2550
PspPPI	RGGWCCY	1 cut(s)	1937
PstI	CTGCAG	1 cut(s)	22
PstNI	CAGNNNCTG	2 cut(s)	17, 26
PsuI	RGATCY	1 cut(s)	1466
RseI	CAYNNNNRTG	2 cut(s)	386, 2006
SacI	GAGCTC	2 cut(s)	585, 1200
SapI	GCTCTTC	1 cut(s)	143
SaqAI	TTAA	6 cut(s)	152, 1736, 2150, 2505, 2523, 2565
Sau3AI	GATC	9 cut(s)	81, 438, 855, 1431, 1466, 1591, 2266, 2428, 2434
Sau96I	GGNCC	8 cut(s)	251, 565, 935, 1046, 1937, 2247, 2441, 2550
SchI	GAGTC	3 cut(s)	686, 880, 2195
SduI	GDGCHC	5 cut(s)	585, 658, 1170, 1200, 2342
SfaNI	GCATC	9 cut(s)	865, 1101, 1128, 1366, 1918, 1993, 2088, 2381, 2400
SfcI	CTRYAG	1 cut(s)	18
SfoI	GGCGCC	2 cut(s)	460, 736
SfuI	TTCGAA	2 cut(s)	790, 1611
SgrAI	CRCCGGYG	1 cut(s)	845
SinI	GGWCC	3 cut(s)	1937, 2247, 2441
SmaI	CCCGGG	1 cut(s)	1990
SmiMI	CAYNNNNRTG	2 cut(s)	386, 2006
SmlI	CTYRAG	1 cut(s)	965
SmoI	CTYRAG	1 cut(s)	965
SpeI	ACTAGT	1 cut(s)	2063
Sse9I	AATT	8 cut(s)	40, 149, 177, 278, 1263, 2089, 2241, 2501
SseBI	AGGCCT	3 cut(s)	1188, 1866, 1955
SspDI	GGCGCC	2 cut(s)	458, 734
SspMI	CTAG	3 cut(s)	746, 1163, 2064
SstI	GAGCTC	2 cut(s)	585, 1200
StuI	AGGCCT	3 cut(s)	1188, 1866, 1955
StyI	CCWWGG	4 cut(s)	505, 526, 702, 2250
TaaI	ACNGT	7 cut(s)	95, 1114, 1312, 1765, 2005, 2103, 2233
TaiI	ACGT	6 cut(s)	339, 720, 1058, 1821, 1917, 2262
TasI	AATT	8 cut(s)	40, 149, 177, 278, 1263, 2089, 2241, 2501
TauI	GCSGC	9 cut(s)	8, 11, 464, 735, 1512, 1515, 1660, 2240, 2532
TfiI	GAWTC	5 cut(s)	391, 925, 1063, 1287, 1848
Tru1I	TTAA	6 cut(s)	152, 1736, 2150, 2505, 2523, 2565
Tru9I	TTAA	6 cut(s)	152, 1736, 2150, 2505, 2523, 2565
TscAI	CASTG	3 cut(s)	18, 393, 823
TseFI	GTSAC	4 cut(s)	166, 544, 868, 1303
Tsp45I	GTSAC	4 cut(s)	166, 544, 868, 1303
TspGWI	ACGGA	6 cut(s)	532, 883, 887, 1831, 2235, 2339
TspMI	CCCGGG	1 cut(s)	1988
TspRI	CASTG	3 cut(s)	18, 393, 823
Van91I	CCANNNNNTGG	2 cut(s)	780, 1498
VpaK11BI	GGWCC	3 cut(s)	1937, 2247, 2441
XapI	RAATTY	2 cut(s)	177, 278
XceI	RCATGY	1 cut(s)	646
XcmI	CCANNNNNNNNNTGG	1 cut(s)	2068
XmaI	CCCGGG	1 cut(s)	1988
XmiI	GTMKAC	2 cut(s)	1314, 1482
XspI	CTAG	3 cut(s)	746, 1163, 2064

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.