Rw2G023310

Cation H( ) antiporter

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_wichuraiana
Chr2
OG0017951
Physical Location & Seq
Reverse (-)
33943863 .. 33947383
3521 bp
Loading structure...
UTR
Exon/CDS
Intron
Rw2G023310.1

Sequence Viewer

Length: 2610 bp
ATGGCGAACGCGACATCGGCCAACGGGACTAATATGACTAATGCATCAGATACTTCAATAGTATGCTATGCCCCAACCATGATAACCACCAATGGTATTTGGCAGGGAGATAACCCTTTAGACTATTCCCTCCCTCTTTTCATCTTGCAGTTGACTCTGGTCGTCGTCACCACTCGCGTTCTCGTCCTCCTTCTCAAACCCTTTCGGCAACCTCGTGTCATCGCAGAAATCATGGGTGGAGTGTTATTGGGTCCATCGGTGCTTGGACAGTATAAACCATTCGCCGACACAATATTCCCCATGAGAAGTGTGATGGTGATCGAGACCATGGCAAATGTGGGTCTCCTCTACTTCCTCTTCTTGGTCGGCTTGGAGATGGACATTTCCGTTATTCGCCGCATGGGCAAAAGAGCCGTTATTATTGCTGTTGCCGGCATGATCCTCCCCTTCGCCATTGGTGCCGCCTTTTCGTTCCTCATCCACGATAGAGGAAAATCAATGAACCAAGGTACCTTCATTCTCTTCCTCGGCGTTGCCCTCTCCGTCACCGCCTTCCCGGTCCTTGCTCGAGTCCTGGCCGAGCTCAAGCTCGTCAACACCGAGCTCGGCAGGATTGCTTTATCCTCGGCACTGGTAAACGACATGTTCGCTTGGGGTCTCTTAGCTCTGGCCATTGCCTTGGCGGAAAATGACTCCACTTCGCTGGCTTCCCTCTATGTTGTTTTGTCTAGCATTGCCTTTGTTTGCTTTTGCATATTCGTGGTCCGACCTGCAGTGTGCTGGATTATCCGAAGAACTCCAGAAGGAGAATCCTTTAGCGAGTTCTACATTTGCCTTATTCTCACCGGGGTCATGATCTCCGGATTTGTTACTGACGCCATAGGAACACATTCCGTTTTTGGGGCTTTCGTGTTTGGTTTAGTCATCCCTAATGGACCACTTGGCGTCACTCTCATCGAGAAGCTCGAAGATTTTGTTTCGGGGCTTCTGCTCCCTCTTTTCTTTGCCATTAGCGGACTTAGAACCAATATTACTACGATCGAAGGAATTGGAACCTGGGGCCTTCTTATACTCGTTATCTTTCTCTCTTGCATCGGAAAAATAGCCGGTACAGTCATTGTCTGCCTCTTTTACCAGATGCCATTTGACGAAGGGTTCACTCTAGGACTGCTCATGAACACAAAAGGCCTCGTCGAATTGATTGTCCTCAATGTCGGCAAAGACCAGAAGGTTCTAGACGATGAGGCCTTTGCAATTATGGTGATTGTAGCTGTTGTTATGACTGGAATCATTACCCCAATAGTAACAACAGTTTACAAGCCGGCAAGAAGGTCTATACCTTACAAACGAAGAACGATTCAAAGGTCGAAACCCGATGCGGAGTTACGAGTACTTGTTTGTGTCCACACACCTCGAAATGTGCCTACAATGATCAACCTTTTAGAAGCCTCTTACCCCACAAAGCGATCTCCTTTGTGCGTCTACGTACTCCATTTAGTTGAGCTCAGTGGCCGTGCATCTGCAATGCTCATTGTTCATAACACTCGGAAATCTGGTAGGCCGGCGCTCAATCGAACCCAAGCTCAGTCTGACCACATCATAAATGCGTTCGAGAACTACGAGCAGCATGCCGGTTGTGTCTCGGTGCAGCCCTTGACTGCAATCTCACCATACTCTACAATGCACGAAGACATTTGCAACTTGGCAGAGGACAAACGTGTTGCCTTTTTAATTCTTCCTTTCCACAAACAGCAAACAGTGGATGGAGGAATGGAATCCATGAACCCGGCTTTTAGAACAATGAACCAAAATGTATTAGCAAATGCGCCATGCTCAGTCGGAATTCTAGTGGATAGAGGTCTCAATGGCTCCAATCGCGTGGCTGCAAACCAGGCATCTCACAATATAGTGGTACTTTTCTTCGGTGGACCGGACGACCGAGAAGCGTTGTCATATGCATGGAGGATGTCTGAACATCCAGGAATAACTCTTGCAGTCATGCGTTTTGTTCCCGGGCCGGACGTCGCGGAGCCGGTAGGAATAGTGAAACCCGATCCTGATGCCGCAAACAACGGCAACAACAATTCAAAGATATTGTGTGTCGAAACGGACGTGGGGTGGGAGAAGCAACTCGACGAGAAGTTGATAAATGAGTTTAGGACACAAAATATGAACGATGAATCAATTGTGTACCTGGAGAAGGAGGTGAACAATGGAGAGGAGACGGTGGCGGTGGTAAGGTCAATGGACAGCGTGCATGACCTATTTATAGTCGGCAGAGGACAAGGCATAATATCGCCATTAACGGCGGGGCTAACCGATTGGAGTGAGTGCCCGGAACTAGGTGCAATTGGGGATTTGCTTGCGTCTTCCGACTTCGCAACAACCGCATCGGTTTTGGTAGTGCAACAATTCGTTGGTGATGAAGGGAATGCAGTAGTCGAACACGAGCTCGAGGATCCCAATGAGGGGCACGGAACACCGCAACCGGTAGCCAGCAGCCCAGCAGATGAACCTCAAAATGCGCAGTTTGGCAACATCCAGCGACATATGAACAAGCGGACTCAACCACCACCTAGGGTACAAAATAGTAACGTGTACAGCCCCTAA

Protein Analysis

869

Amino Acids

94.5

Weight (kDa)

5.44

Isoelectric Point (pI)

34.46

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Na_H_Exchanger PF00999 59 - 436 2.3e-64 Sodium/hydrogen exchanger, transmembrane
CHX17_2nd PF23256 491 - 630 2e-31 Plant cation/H(+) antiporter domain
CHX17_C PF23259 635 - 676 1.3e-08 Plant cation/H(+) antiporter, C-terminal domain
CHX17_C PF23259 711 - 807 2.4e-09 Plant cation/H(+) antiporter, C-terminal domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0017951)

Species Orthologous Gene IDs
arabidopsis_thaliana AT2G13620
fragaria_vesca FvH4_1g24460
malus_domestica MD01G1005100.v1.1
prunus_persica Prupe.4G271900_v2.0.a1
rosa_chinensis RchiOBHm_Chr2g0121601
rosa_laevigata RLG00000018626
rosa_roxburghii Rroxscaffold_2G00122330
rosa_rugosa Rorug02G0233800
rosa_samantha Rh2BG301500 Rh2CG280300
rosa_wichuraiana Rw2G023310

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AatII GACGTC 1 cut(s) 2025
Acc16I TGCGCA 1 cut(s) 2526
Acc36I ACCTGC 1 cut(s) 778
Acc65I GGTACC 1 cut(s) 509
AccB1I GGYRCC 2 cut(s) 458, 509
AccI GTMKAC 1 cut(s) 1482
AccII CGCG 4 cut(s) 11, 177, 1878, 2027
AccIII TCCGGA 1 cut(s) 860
AclWI GGATC 4 cut(s) 433, 2048, 2453, 2466
AcoI YGGCCR 4 cut(s) 18, 576, 669, 1510
AcsI RAATTY 1 cut(s) 1842
AcyI GRCGYC 3 cut(s) 876, 945, 2022
AfaI GTAC 8 cut(s) 511, 1111, 1392, 1488, 1914, 2192, 2583, 2600
AfiI CCNNNNNNNGG 6 cut(s) 361, 900, 2200, 2342, 2468, 2469
AflIII ACRYGT 3 cut(s) 642, 1717, 2595
AgeI ACCGGT 1 cut(s) 2488
AgsI TTSAA 3 cut(s) 57, 1361, 2088
AjiI CACGTC 1 cut(s) 2113
AjnI CCWGG 5 cut(s) 573, 1055, 1890, 1978, 2193
AluBI AGCT 9 cut(s) 583, 589, 604, 665, 964, 1271, 1504, 1583, 2452
AluI AGCT 9 cut(s) 583, 589, 604, 665, 964, 1271, 1504, 1583, 2452
Alw21I GWGCWC 4 cut(s) 585, 606, 1506, 2454
Alw26I GTCTC 6 cut(s) 317, 347, 662, 1645, 1865, 2216
AlwI GGATC 4 cut(s) 433, 2048, 2453, 2466
Ama87I CYCGRG 3 cut(s) 567, 2012, 2453
Aor13HI TCCGGA 1 cut(s) 860
AoxI GGCC 9 cut(s) 18, 576, 669, 1060, 1186, 1245, 1510, 1559, 2015
ApeKI GCWGC 4 cut(s) 1624, 1648, 1883, 2499
ApoI RAATTY 1 cut(s) 1842
ArsI GACNNNNNNTTYG 2 cut(s) 1188, 1220
AsiGI ACCGGT 1 cut(s) 2488
Asp700I GAANNNNTTC 1 cut(s) 889
Asp718I GGTACC 1 cut(s) 509
AspA2I CCTAGG 1 cut(s) 2576
AspLEI GCGC 3 cut(s) 1567, 1828, 2527
AspS9I GGNCC 7 cut(s) 251, 559, 763, 935, 1060, 1928, 2015
AsuC2I CCSGG 6 cut(s) 557, 847, 1787, 2013, 2014, 2336
AsuHPI GGTGA 8 cut(s) 160, 328, 538, 835, 1273, 1659, 2218, 2432
AvaI CYCGRG 3 cut(s) 567, 2012, 2453
AvaII GGWCC 5 cut(s) 251, 559, 763, 935, 1928
AvrII CCTAGG 1 cut(s) 2576
BaeGI GKGCMC 2 cut(s) 2336, 2475
BaeI ACNNNNGTAYC 2 cut(s) 493, 526
BalI TGGCCA 1 cut(s) 671
BamHI GGATCC 1 cut(s) 2458
BanI GGYRCC 2 cut(s) 458, 509
BanII GRGCYC 4 cut(s) 585, 606, 1506, 2454
BauI CACGAG 2 cut(s) 213, 2447
BbsI GAAGAC 2 cut(s) 1695, 2361
Bbv12I GWGCWC 4 cut(s) 585, 606, 1506, 2454
BbvI GCAGC 4 cut(s) 1636, 1660, 1870, 2511
BccI CCATC 4 cut(s) 262, 307, 370, 1757
BceAI ACGGC 4 cut(s) 398, 1497, 2089, 2322
BcgI CGANNNNNNTGC 4 cut(s) 1610, 1644, 2042, 2076
BciT130I CCWGG 5 cut(s) 575, 1057, 1892, 1980, 2195
BclI TGATCA 1 cut(s) 1431
BcnI CCSGG 6 cut(s) 557, 847, 1787, 2013, 2014, 2336
BcoDI GTCTC 6 cut(s) 317, 347, 662, 1645, 1865, 2216
BfaI CTAG 6 cut(s) 729, 1163, 1235, 1847, 2342, 2577
BfmI CTRYAG 1 cut(s) 771
BfoI RGCGCY 1 cut(s) 1568
BfuAI ACCTGC 1 cut(s) 778
BglI GCCNNNNNGGC 1 cut(s) 402
BisI GCNGC 7 cut(s) 397, 462, 1625, 1649, 1884, 2064, 2500
BlnI CCTAGG 1 cut(s) 2576
BlsI GCNGC 7 cut(s) 398, 463, 1626, 1650, 1885, 2065, 2501
BmcAI AGTACT 1 cut(s) 1392
Bme18I GGWCC 5 cut(s) 251, 559, 763, 935, 1928
BmeT110I CYCGRG 3 cut(s) 567, 2012, 2453
BmgBI CACGTC 1 cut(s) 2113
BmgT120I GGNCC 7 cut(s) 251, 559, 763, 935, 1060, 1928, 2015
BmiI GGNNCC 8 cut(s) 252, 460, 511, 1054, 1061, 1870, 2031, 2460
BmsI GCATC 8 cut(s) 53, 1101, 1128, 1366, 1526, 1904, 2050, 2399
BoxI GACNNNNGTC 1 cut(s) 158
BpiI GAAGAC 2 cut(s) 1695, 2361
BpmI CTGGAG 2 cut(s) 783, 2216
BpuEI CTTGAG 1 cut(s) 569
BpuMI CCSGG 6 cut(s) 557, 847, 1787, 2013, 2014, 2336
BsaAI YACGTR 1 cut(s) 1486
BsaBI GATNNNNATC 1 cut(s) 317
BsaHI GRCGYC 3 cut(s) 876, 945, 2022
BsaI GGTCTC 4 cut(s) 317, 347, 662, 1865
BsaJI CCNNGG 9 cut(s) 327, 505, 526, 624, 678, 846, 1056, 2012, 2576
BsaWI WCCGGW 3 cut(s) 860, 1930, 2488
Bsc4I CCNNNNNNNGG 6 cut(s) 361, 900, 2200, 2342, 2468, 2469
Bse118I RCCGGY 7 cut(s) 431, 1106, 1321, 1561, 1631, 2032, 2488
Bse1I ACTGG 2 cut(s) 636, 1288
Bse3DI GCAATG 3 cut(s) 672, 732, 1530
Bse8I GATNNNNATC 1 cut(s) 317
BseAI TCCGGA 1 cut(s) 860
BseBI CCWGG 5 cut(s) 575, 1057, 1892, 1980, 2195
BseDI CCNNGG 9 cut(s) 327, 505, 526, 624, 678, 846, 1056, 2012, 2576
BseGI GGATG 6 cut(s) 477, 924, 1768, 1971, 1975, 2538
BseJI GATNNNNATC 1 cut(s) 317
BseLI CCNNNNNNNGG 6 cut(s) 361, 900, 2200, 2342, 2468, 2469
BseMI GCAATG 3 cut(s) 672, 732, 1530
BseMII CTCAG 3 cut(s) 1519, 1598, 1848
BseNI ACTGG 2 cut(s) 636, 1288
BseRI GAGGAG 2 cut(s) 335, 2234
BseSI GKGCMC 2 cut(s) 2336, 2475
BseXI GCAGC 4 cut(s) 1636, 1660, 1870, 2511
BseYI CCCAGC 1 cut(s) 2503
BsgI GTGCAG 1 cut(s) 1667
Bsh1236I CGCG 4 cut(s) 11, 177, 1878, 2027
Bsh1285I CGRYCG 2 cut(s) 1041, 1939
BshFI GGCC 9 cut(s) 20, 578, 671, 1062, 1188, 1247, 1512, 1561, 2017
BshNI GGYRCC 2 cut(s) 458, 509
BshTI ACCGGT 1 cut(s) 2488
BsiEI CGRYCG 2 cut(s) 1041, 1939
BsiHKAI GWGCWC 4 cut(s) 585, 606, 1506, 2454
BsiHKCI CYCGRG 3 cut(s) 567, 2012, 2453
BslFI GGGAC 1 cut(s) 40
BslI CCNNNNNNNGG 6 cut(s) 361, 900, 2200, 2342, 2468, 2469
BsmAI GTCTC 6 cut(s) 317, 347, 662, 1645, 1865, 2216
BsmBI CGTCTC 1 cut(s) 2216
BsmFI GGGAC 1 cut(s) 40
BsmI GAATGC 1 cut(s) 2437
BsnI GGCC 9 cut(s) 20, 578, 671, 1062, 1188, 1247, 1512, 1561, 2017
Bso31I GGTCTC 4 cut(s) 317, 347, 662, 1865
BsoBI CYCGRG 3 cut(s) 567, 2012, 2453
Bsp1286I GDGCHC 6 cut(s) 585, 606, 1506, 2336, 2454, 2475
Bsp13I TCCGGA 1 cut(s) 860
Bsp1407I TGTACA 1 cut(s) 2598
Bsp143I GATC 8 cut(s) 318, 438, 855, 1038, 1431, 1466, 2053, 2458
Bsp19I CCATGG 1 cut(s) 327
BspANI GGCC 9 cut(s) 20, 578, 671, 1062, 1188, 1247, 1512, 1561, 2017
BspCNI CTCAG 3 cut(s) 1518, 1597, 1847
BspEI TCCGGA 1 cut(s) 860
BspFNI CGCG 4 cut(s) 11, 177, 1878, 2027
BspHI TCATGA 2 cut(s) 852, 1173
BspLI GGNNCC 8 cut(s) 252, 460, 511, 1054, 1061, 1870, 2031, 2460
BspMAI CTGCAG 1 cut(s) 775
BspMI ACCTGC 1 cut(s) 778
BspPI GGATC 4 cut(s) 433, 2048, 2453, 2466
BspT107I GGYRCC 2 cut(s) 458, 509
BspTNI GGTCTC 4 cut(s) 317, 347, 662, 1865
BsrDI GCAATG 3 cut(s) 672, 732, 1530
BsrFI RCCGGY 7 cut(s) 431, 1106, 1321, 1561, 1631, 2032, 2488
BsrGI TGTACA 1 cut(s) 2598
BsrI ACTGG 2 cut(s) 636, 1288
BssAI RCCGGY 7 cut(s) 431, 1106, 1321, 1561, 1631, 2032, 2488
BssECI CCNNGG 9 cut(s) 327, 505, 526, 624, 678, 846, 1056, 2012, 2576
BssMI GATC 8 cut(s) 318, 438, 855, 1038, 1431, 1466, 2053, 2458
BssNI GRCGYC 3 cut(s) 876, 945, 2022
BssSI CACGAG 2 cut(s) 213, 2447
BssT1I CCWWGG 4 cut(s) 327, 505, 678, 2576
Bst2BI CACGAG 2 cut(s) 213, 2447
Bst2UI CCWGG 5 cut(s) 575, 1057, 1892, 1980, 2195
Bst4CI ACNGT 5 cut(s) 270, 1114, 1312, 1759, 2227
Bst6I CTCTTC 2 cut(s) 362, 527
BstACI GRCGYC 3 cut(s) 876, 945, 2022
BstAUI TGTACA 1 cut(s) 2598
BstBAI YACGTR 1 cut(s) 1486
BstC8I GCNNGC 8 cut(s) 433, 705, 1323, 1563, 1629, 2255, 2364, 2497
BstDEI CTNAG 5 cut(s) 661, 1019, 1505, 1584, 1834
BstDSI CCRYGG 1 cut(s) 327
BstENI CCTNNNNNAGG 1 cut(s) 2198
BstF5I GGATG 6 cut(s) 477, 924, 1768, 1971, 1975, 2538
BstFNI CGCG 4 cut(s) 11, 177, 1878, 2027
BstH2I RGCGCY 1 cut(s) 1568
BstHHI GCGC 3 cut(s) 1567, 1828, 2527
BstKTI GATC 8 cut(s) 321, 441, 858, 1041, 1434, 1469, 2056, 2461
BstMAI GTCTC 6 cut(s) 317, 347, 662, 1645, 1865, 2216
BstMBI GATC 8 cut(s) 318, 438, 855, 1038, 1431, 1466, 2053, 2458
BstMCI CGRYCG 2 cut(s) 1041, 1939
BstMWI GCNNNNNNNGC 6 cut(s) 17, 402, 458, 1875, 1892, 2387
BstNI CCWGG 5 cut(s) 575, 1057, 1892, 1980, 2195
BstNSI RCATGY 2 cut(s) 646, 1631
BstPAI GACNNNNGTC 1 cut(s) 158
BstSFI CTRYAG 1 cut(s) 771
BstSLI GKGCMC 2 cut(s) 2336, 2475
BstSNI TACGTA 1 cut(s) 1486
BstUI CGCG 4 cut(s) 11, 177, 1878, 2027
BstV1I GCAGC 4 cut(s) 1636, 1660, 1870, 2511
BstV2I GAAGAC 2 cut(s) 1695, 2361
BstX2I RGATCY 1 cut(s) 2458
BstXI CCANNNNNNTGG 3 cut(s) 679, 703, 1879
BstYI RGATCY 1 cut(s) 2458
BsuRI GGCC 9 cut(s) 20, 578, 671, 1062, 1188, 1247, 1512, 1561, 2017
BtgI CCRYGG 1 cut(s) 327
BtgZI GCGATG 1 cut(s) 205
BtrI CACGTC 1 cut(s) 2113
BtsCI GGATG 6 cut(s) 477, 924, 1768, 1971, 1975, 2538
BtsI GCAGTG 1 cut(s) 780
BtsIMutI CAGTG 4 cut(s) 629, 780, 1513, 1764
BveI ACCTGC 1 cut(s) 778
Cac8I GCNNGC 8 cut(s) 433, 705, 1323, 1563, 1629, 2255, 2364, 2497
CciI TCATGA 2 cut(s) 852, 1173
CfoI GCGC 3 cut(s) 1567, 1828, 2527
Cfr10I RCCGGY 7 cut(s) 431, 1106, 1321, 1561, 1631, 2032, 2488
Cfr13I GGNCC 7 cut(s) 251, 559, 763, 935, 1060, 1928, 2015
Cfr9I CCCGGG 1 cut(s) 2012
CseI GACGC 4 cut(s) 884, 934, 1468, 2355
Csp6I GTAC 8 cut(s) 510, 1110, 1391, 1487, 1913, 2191, 2582, 2599
CspAI ACCGGT 1 cut(s) 2488
CviQI GTAC 8 cut(s) 510, 1110, 1391, 1487, 1913, 2191, 2582, 2599
DdeI CTNAG 5 cut(s) 661, 1019, 1505, 1584, 1834
DpnI GATC 8 cut(s) 320, 440, 857, 1040, 1433, 1468, 2055, 2460
DpnII GATC 8 cut(s) 318, 438, 855, 1038, 1431, 1466, 2053, 2458
EaeI YGGCCR 4 cut(s) 18, 576, 669, 1510
Eam1104I CTCTTC 2 cut(s) 362, 527
EarI CTCTTC 2 cut(s) 362, 527
EciI GGCGGA 1 cut(s) 698
Ecl136II GAGCTC 4 cut(s) 583, 604, 1504, 2452
Eco105I TACGTA 1 cut(s) 1486
Eco130I CCWWGG 4 cut(s) 327, 505, 678, 2576
Eco147I AGGCCT 2 cut(s) 1188, 1247
Eco24I GRGCYC 4 cut(s) 585, 606, 1506, 2454
Eco31I GGTCTC 4 cut(s) 317, 347, 662, 1865
Eco47I GGWCC 5 cut(s) 251, 559, 763, 935, 1928
Eco53kI GAGCTC 4 cut(s) 583, 604, 1504, 2452
Eco88I CYCGRG 3 cut(s) 567, 2012, 2453
EcoICRI GAGCTC 4 cut(s) 583, 604, 1504, 2452
EcoNI CCTNNNNNAGG 1 cut(s) 2198
EcoO109I RGGNCCY 1 cut(s) 1060
EcoRI GAATTC 1 cut(s) 1842
EcoRII CCWGG 5 cut(s) 573, 1055, 1890, 1978, 2193
EcoT14I CCWWGG 4 cut(s) 327, 505, 678, 2576
EcoT22I ATGCAT 2 cut(s) 46, 1960
EcoT38I GRGCYC 4 cut(s) 585, 606, 1506, 2454
ErhI CCWWGG 4 cut(s) 327, 505, 678, 2576
Esp3I CGTCTC 1 cut(s) 2216
FaqI GGGAC 1 cut(s) 40
FauI CCCGC 1 cut(s) 2302
FauNDI CATATG 2 cut(s) 1954, 2550
FbaI TGATCA 1 cut(s) 1431
FblI GTMKAC 1 cut(s) 1482
Fnu4HI GCNGC 7 cut(s) 397, 462, 1625, 1649, 1884, 2064, 2500
FokI GGATG 6 cut(s) 464, 911, 1775, 1962, 1978, 2525
FriOI GRGCYC 4 cut(s) 585, 606, 1506, 2454
Fsp4HI GCNGC 7 cut(s) 397, 462, 1625, 1649, 1884, 2064, 2500
FspBI CTAG 6 cut(s) 729, 1163, 1235, 1847, 2342, 2577
FspI TGCGCA 1 cut(s) 2526
GlaI GCGC 3 cut(s) 1566, 1827, 2526
GluI GCNGC 7 cut(s) 397, 462, 1625, 1649, 1884, 2064, 2500
GsaI CCCAGC 1 cut(s) 2507
GsuI CTGGAG 2 cut(s) 783, 2216
HaeII RGCGCY 1 cut(s) 1568
HaeIII GGCC 9 cut(s) 20, 578, 671, 1062, 1188, 1247, 1512, 1561, 2017
HgaI GACGC 4 cut(s) 884, 934, 1468, 2355
HhaI GCGC 3 cut(s) 1567, 1828, 2527
Hin1I GRCGYC 3 cut(s) 876, 945, 2022
Hin6I GCGC 3 cut(s) 1565, 1826, 2525
HinP1I GCGC 3 cut(s) 1565, 1826, 2525
HincII GTYRAC 2 cut(s) 153, 595
HindII GTYRAC 2 cut(s) 153, 595
HinfI GANTC 9 cut(s) 154, 570, 692, 809, 1287, 1357, 1775, 2180, 2563
HphI GGTGA 8 cut(s) 160, 328, 538, 835, 1273, 1659, 2218, 2432
Hpy188I TCNGA 9 cut(s) 49, 767, 791, 1097, 1548, 1591, 1841, 1972, 2374
Hpy188III TCNNGA 9 cut(s) 322, 800, 853, 861, 958, 1174, 1235, 1612, 2057
Hpy99I CGWCG 4 cut(s) 167, 1196, 2027, 2138
HpyCH4III ACNGT 5 cut(s) 270, 1114, 1312, 1759, 2227
HpyCH4IV ACGT 5 cut(s) 1485, 1717, 2022, 2112, 2595
HpyF10VI GCNNNNNNNGC 6 cut(s) 17, 402, 458, 1875, 1892, 2387
HpyF3I CTNAG 5 cut(s) 661, 1019, 1505, 1584, 1834
HpySE526I ACGT 5 cut(s) 1485, 1717, 2022, 2112, 2595
Hsp92I GRCGYC 3 cut(s) 876, 945, 2022
HspAI GCGC 3 cut(s) 1565, 1826, 2525
Kpn2I TCCGGA 1 cut(s) 860
KpnI GGTACC 1 cut(s) 513
KroI GCCGGC 3 cut(s) 431, 1321, 1561
KroNI GCCGGC 3 cut(s) 433, 1323, 1563
Ksp22I TGATCA 1 cut(s) 1431
Kzo9I GATC 8 cut(s) 318, 438, 855, 1038, 1431, 1466, 2053, 2458
LmnI GCTCC 3 cut(s) 996, 1874, 2029
Lsp1109I GCAGC 4 cut(s) 1636, 1660, 1870, 2511
LweI GCATC 8 cut(s) 53, 1101, 1128, 1366, 1526, 1904, 2050, 2399
MaeI CTAG 6 cut(s) 729, 1163, 1235, 1847, 2342, 2577
MaeII ACGT 5 cut(s) 1485, 1717, 2022, 2112, 2595
MaeIII GTNAC 7 cut(s) 166, 544, 868, 946, 1303, 1383, 2591
MalI GATC 8 cut(s) 320, 440, 857, 1040, 1433, 1468, 2055, 2460
MboI GATC 8 cut(s) 318, 438, 855, 1038, 1431, 1466, 2053, 2458
MboII GAAGA 9 cut(s) 349, 514, 804, 980, 1362, 1700, 1727, 1912, 2361
MfeI CAATTG 2 cut(s) 2184, 2349
MflI RGATCY 1 cut(s) 2458
MhlI GDGCHC 6 cut(s) 585, 606, 1506, 2336, 2454, 2475
MlsI TGGCCA 1 cut(s) 671
MluCI AATT 9 cut(s) 1047, 1196, 1254, 1731, 1842, 2083, 2184, 2349, 2411
MluNI TGGCCA 1 cut(s) 671
MlyI GAGTC 4 cut(s) 148, 579, 686, 2557
MmeI TCCRAC 3 cut(s) 790, 1819, 2397
Mox20I TGGCCA 1 cut(s) 671
Mph1103I ATGCAT 2 cut(s) 46, 1960
MroI TCCGGA 1 cut(s) 860
MroNI GCCGGC 3 cut(s) 431, 1321, 1561
MroXI GAANNNNTTC 1 cut(s) 889
MscI TGGCCA 1 cut(s) 671
MseI TTAA 2 cut(s) 1730, 2303
MslI CAYNNNNRTG 2 cut(s) 758, 1957
Msp20I TGGCCA 1 cut(s) 671
MunI CAATTG 2 cut(s) 2184, 2349
Mva1269I GAATGC 1 cut(s) 2437
MvaI CCWGG 5 cut(s) 575, 1057, 1892, 1980, 2195
MvnI CGCG 4 cut(s) 11, 177, 1878, 2027
MwoI GCNNNNNNNGC 6 cut(s) 17, 402, 458, 1875, 1892, 2387
NaeI GCCGGC 3 cut(s) 433, 1323, 1563
NciI CCSGG 6 cut(s) 557, 847, 1787, 2013, 2014, 2336
NcoI CCATGG 1 cut(s) 327
NdeI CATATG 2 cut(s) 1954, 2550
NdeII GATC 8 cut(s) 318, 438, 855, 1038, 1431, 1466, 2053, 2458
NgoMIV GCCGGC 3 cut(s) 431, 1321, 1561
NlaIV GGNNCC 8 cut(s) 252, 460, 511, 1054, 1061, 1870, 2031, 2460
NmeAIII GCCGAG 4 cut(s) 507, 585, 604, 605
NmuCI GTSAC 3 cut(s) 166, 544, 946
NsbI TGCGCA 1 cut(s) 2526
NsiI ATGCAT 2 cut(s) 46, 1960
NspI RCATGY 2 cut(s) 646, 1631
PaeI GCATGC 1 cut(s) 1631
PaeR7I CTCGAG 2 cut(s) 567, 2453
PagI TCATGA 2 cut(s) 852, 1173
PceI AGGCCT 2 cut(s) 1188, 1247
PciI ACATGT 1 cut(s) 642
PcsI WCGNNNNNNNCGW 5 cut(s) 211, 597, 963, 1617, 2109
PctI GAATGC 1 cut(s) 2437
PdiI GCCGGC 3 cut(s) 433, 1323, 1563
PdmI GAANNNNTTC 1 cut(s) 889
PfeI GAWTC 5 cut(s) 809, 1287, 1357, 1775, 2180
PfoI TCCNGGA 1 cut(s) 1978
PinAI ACCGGT 1 cut(s) 2488
PkrI GCNGC 7 cut(s) 398, 463, 1626, 1650, 1885, 2065, 2501
Ple19I CGATCG 1 cut(s) 1041
PleI GAGTC 4 cut(s) 148, 578, 686, 2557
PpsI GAGTC 4 cut(s) 148, 578, 686, 2557
Ppu21I YACGTR 1 cut(s) 1486
PscI ACATGT 1 cut(s) 642
PshAI GACNNNNGTC 1 cut(s) 158
Psp124BI GAGCTC 4 cut(s) 585, 606, 1506, 2454
Psp6I CCWGG 5 cut(s) 573, 1055, 1890, 1978, 2193
PspFI CCCAGC 1 cut(s) 2503
PspGI CCWGG 5 cut(s) 573, 1055, 1890, 1978, 2193
PspN4I GGNNCC 8 cut(s) 252, 460, 511, 1054, 1061, 1870, 2031, 2460
PspPI GGNCC 7 cut(s) 251, 559, 763, 935, 1060, 1928, 2015
PspXI VCTCGAGB 2 cut(s) 567, 2453
PsrI GAACNNNNNNTAC 2 cut(s) 1015, 1047
PstI CTGCAG 1 cut(s) 775
PsuI RGATCY 1 cut(s) 2458
PvuI CGATCG 1 cut(s) 1041
RsaI GTAC 8 cut(s) 511, 1111, 1392, 1488, 1914, 2192, 2583, 2600
RsaNI GTAC 8 cut(s) 510, 1110, 1391, 1487, 1913, 2191, 2582, 2599
RseI CAYNNNNRTG 2 cut(s) 758, 1957
SacI GAGCTC 4 cut(s) 585, 606, 1506, 2454
SaqAI TTAA 2 cut(s) 1730, 2303
SatI GCNGC 7 cut(s) 397, 462, 1625, 1649, 1884, 2064, 2500
Sau3AI GATC 8 cut(s) 318, 438, 855, 1038, 1431, 1466, 2053, 2458
Sau96I GGNCC 7 cut(s) 251, 559, 763, 935, 1060, 1928, 2015
ScaI AGTACT 1 cut(s) 1392
SchI GAGTC 4 cut(s) 148, 579, 686, 2557
SduI GDGCHC 6 cut(s) 585, 606, 1506, 2336, 2454, 2475
SfaNI GCATC 8 cut(s) 53, 1101, 1128, 1366, 1526, 1904, 2050, 2399
SfcI CTRYAG 1 cut(s) 771
Sfr274I CTCGAG 2 cut(s) 567, 2453
SinI GGWCC 5 cut(s) 251, 559, 763, 935, 1928
SlaI CTCGAG 2 cut(s) 567, 2453
SmaI CCCGGG 1 cut(s) 2014
SmiMI CAYNNNNRTG 2 cut(s) 758, 1957
SmlI CTYRAG 3 cut(s) 567, 584, 2453
SmoI CTYRAG 3 cut(s) 567, 584, 2453
SnaBI TACGTA 1 cut(s) 1486
SphI GCATGC 1 cut(s) 1631
Sse9I AATT 9 cut(s) 1047, 1196, 1254, 1731, 1842, 2083, 2184, 2349, 2411
SseBI AGGCCT 2 cut(s) 1188, 1247
SspI AATATT 2 cut(s) 294, 1030
SspMI CTAG 6 cut(s) 729, 1163, 1235, 1847, 2342, 2577
SstI GAGCTC 4 cut(s) 585, 606, 1506, 2454
StuI AGGCCT 2 cut(s) 1188, 1247
StyI CCWWGG 4 cut(s) 327, 505, 678, 2576
TaaI ACNGT 5 cut(s) 270, 1114, 1312, 1759, 2227
TaiI ACGT 5 cut(s) 1488, 1720, 2025, 2115, 2598
TaqII GACCGA 1 cut(s) 1953
TasI AATT 9 cut(s) 1047, 1196, 1254, 1731, 1842, 2083, 2184, 2349, 2411
TatI WGTACW 2 cut(s) 1390, 2598
TauI GCSGC 3 cut(s) 399, 464, 2066
TfiI GAWTC 5 cut(s) 809, 1287, 1357, 1775, 2180
Tru1I TTAA 2 cut(s) 1730, 2303
Tru9I TTAA 2 cut(s) 1730, 2303
TscAI CASTG 4 cut(s) 636, 780, 1513, 1764
TseFI GTSAC 3 cut(s) 166, 544, 946
TseI GCWGC 4 cut(s) 1624, 1648, 1883, 2499
Tsp45I GTSAC 3 cut(s) 166, 544, 946
TspGWI ACGGA 5 cut(s) 376, 532, 883, 2123, 2490
TspMI CCCGGG 1 cut(s) 2012
TspRI CASTG 4 cut(s) 636, 780, 1513, 1764
VpaK11BI GGWCC 5 cut(s) 251, 559, 763, 935, 1928
XagI CCTNNNNNAGG 1 cut(s) 2198
XapI RAATTY 1 cut(s) 1842
XbaI TCTAGA 1 cut(s) 1234
XceI RCATGY 2 cut(s) 646, 1631
XcmI CCANNNNNNNNNTGG 1 cut(s) 334
XhoI CTCGAG 2 cut(s) 567, 2453
XmaI CCCGGG 1 cut(s) 2012
XmaJI CCTAGG 1 cut(s) 2576
XmiI GTMKAC 1 cut(s) 1482
XmnI GAANNNNTTC 1 cut(s) 889
XspI CTAG 6 cut(s) 729, 1163, 1235, 1847, 2342, 2577
ZraI GACGTC 1 cut(s) 2023
ZrmI AGTACT 1 cut(s) 1392
Zsp2I ATGCAT 2 cut(s) 46, 1960
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.