FvH4_1g25210

phospholipase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb1
OG0017969
Physical Location & Seq
Reverse (-)
17007659 .. 17009280
1622 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_1g25210.t1

Sequence Viewer

Length: 1047 bp
ATGGGTAGCACAACCAAAAGGTGGAGGCAACTCAGCGGCGAGAATGATTGGGAGGGTCTACTGCACCCTCTAGACATCGATCTCCGCCGCTACATAATCCACTACGGTGAAAGGACCGGAGCAAGCAGAGACGCAGTCATTACCGAGCGAAAATCAAAGAACTACGGACAACCCCGATACGCGAAGGGACATTTCTTCTCTAAGGTGGGTTTGGAGATCGGTAATCCATACAAATATGTGGTGAAAAGGTACCTGTATGCAACACCAAAACTACGGTCGTCGCCGGGGATGTCAAGTGTGATGGGTTACGTTGCGGTGACAACCGATGAAGGAAGCAAAGTGATGGGGAGGAGAGACGTTCTCATTTCGTTTAGAGGAGCTGTGCTGGGATATGAGAAGCTTGGTAATACCAAAGCTCAAGCTGCAGTAAGAGTACTATTGGGTGAATACAAGGACGAGGAAATCAGCATTACTGTCACTGGCCATAGCATGGGTGCTGCATTTGCAGCTCAAACTGCAGTAGACCTCGTTCGCAATGGATTGAATATCATAAAACCTACAGGAATACTACCAGTGATGAAAGCATGTCTGGTCACAGCCATCTTGTTTGCCTGTCACCCTCCCCGCCTTTTAGGACCCGTAAATGCATTTTCGGGTCTCGAAAATCTTCACATCTTGCGCGTCCAAAACAAATATGATAACCCTAATGAGCACTCTGATATTGGAATAGAGTTGAAAATCGACACTTTGAAATCACCCTACTTGAACAATGCACTACAAAACGTGCACGACTTGCAGAATTACTTGCATGGAGTTGCAGGGAAGCAAGGGGAAGGCGATTTTAGGCTCGAAGTTGATCGTGATCTTGCGCTGGTAAACAAATATGTGGATGGTCTGAAGGACGAGTATAATGTAGTTGCGAGGTGGTGGACAGAAAAGAACAAGTCTATGGTTCAAATGGATGATGCCTCTTGGGTGTTAACAGATCATGAGAATGATGATGATGATGATGAAGGAAACAGATCAAGCTTGCGAAGCCGAATATAA

Protein Analysis

349

Amino Acids

39.01

Weight (kDa)

7.76

Isoelectric Point (pI)

40.84

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Lipase_3 PF01764 136 - 182 8e-08 Lipase (class 3)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0017969)

Species Orthologous Gene IDs
fragaria_vesca FvH4_1g25210
rosa_chinensis RchiOBHm_Chr2g0122911
rosa_laevigata RLG00000018725
rosa_multiflora Rmu_sc0001635.1_g000003
rosa_roxburghii Rroxscaffold_2G00120960
rosa_rugosa Rorug02G0243000
rosa_samantha Rh2AG302100 Rh2BG309600 Rh2CG288700 Rh2DG326100
rosa_wichuraiana Rw2G024000

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc65I GGTACC 1 cut(s) 249
AccB1I GGYRCC 1 cut(s) 249
AccB7I CCANNNNNTGG 2 cut(s) 21, 490
AccI GTMKAC 2 cut(s) 58, 522
AccII CGCG 2 cut(s) 182, 681
AciI CCGC 5 cut(s) 36, 85, 88, 314, 625
AcoI YGGCCR 1 cut(s) 481
AcuI CTGAAG 1 cut(s) 917
AfaI GTAC 2 cut(s) 251, 435
AfiI CCNNNNNNNGG 2 cut(s) 21, 490
AgsI TTSAA 5 cut(s) 544, 736, 751, 766, 956
AleI CACNNNNGTG 1 cut(s) 105
AluBI AGCT 6 cut(s) 380, 400, 416, 422, 509, 1029
AluI AGCT 6 cut(s) 380, 400, 416, 422, 509, 1029
Alw21I GWGCWC 2 cut(s) 714, 789
Alw26I GTCTC 3 cut(s) 123, 348, 662
Alw44I GTGCAC 1 cut(s) 785
AoxI GGCC 1 cut(s) 481
ApaLI GTGCAC 1 cut(s) 785
ApeKI GCWGC 3 cut(s) 422, 497, 506
ArsI GACNNNNNNTTYG 2 cut(s) 260, 292
Asp700I GAANNNNTTC 1 cut(s) 666
Asp718I GGTACC 1 cut(s) 249
AspLEI GCGC 2 cut(s) 681, 871
AspS9I GGNCC 2 cut(s) 114, 635
AsuC2I CCSGG 1 cut(s) 285
AsuHPI GGTGA 6 cut(s) 119, 253, 328, 455, 608, 747
AvaII GGWCC 2 cut(s) 114, 635
BaeGI GKGCMC 1 cut(s) 789
BalI TGGCCA 1 cut(s) 483
BanI GGYRCC 1 cut(s) 249
Bbv12I GWGCWC 2 cut(s) 714, 789
BbvI GCAGC 3 cut(s) 409, 484, 518
BccI CCATC 4 cut(s) 295, 337, 608, 884
BcnI CCSGG 1 cut(s) 285
BcoDI GTCTC 3 cut(s) 123, 348, 662
BfaI CTAG 1 cut(s) 71
BfmI CTRYAG 3 cut(s) 423, 516, 558
BisI GCNGC 5 cut(s) 37, 88, 423, 498, 507
BlsI GCNGC 5 cut(s) 38, 89, 424, 499, 508
BmcAI AGTACT 1 cut(s) 435
Bme1390I CCNGG 1 cut(s) 285
Bme18I GGWCC 2 cut(s) 114, 635
BmgT120I GGNCC 2 cut(s) 114, 635
BmiI GGNNCC 2 cut(s) 251, 637
BmrFI CCNGG 1 cut(s) 285
BmsI GCATC 1 cut(s) 955
BplI GAGNNNNNCTC 2 cut(s) 345, 377
BpuEI CTTGAG 1 cut(s) 402
BpuMI CCSGG 1 cut(s) 285
Bsa29I ATCGAT 1 cut(s) 78
BsaBI GATNNNNATC 1 cut(s) 861
BsaI GGTCTC 1 cut(s) 662
BsaJI CCNNGG 1 cut(s) 284
BsaWI WCCGGW 1 cut(s) 116
Bsc4I CCNNNNNNNGG 2 cut(s) 21, 490
Bse1I ACTGG 2 cut(s) 484, 572
Bse3DI GCAATG 1 cut(s) 541
Bse8I GATNNNNATC 1 cut(s) 861
BseCI ATCGAT 1 cut(s) 78
BseDI CCNNGG 1 cut(s) 284
BseGI GGATG 3 cut(s) 294, 895, 967
BseJI GATNNNNATC 1 cut(s) 861
BseLI CCNNNNNNNGG 2 cut(s) 21, 490
BseMI GCAATG 1 cut(s) 541
BseMII CTCAG 1 cut(s) 46
BseNI ACTGG 2 cut(s) 484, 572
BseRI GAGGAG 2 cut(s) 364, 390
BseSI GKGCMC 1 cut(s) 789
BseXI GCAGC 3 cut(s) 409, 484, 518
BseYI CCCAGC 1 cut(s) 385
BsgI GTGCAG 1 cut(s) 47
Bsh1236I CGCG 2 cut(s) 182, 681
Bsh1285I CGRYCG 1 cut(s) 278
BshFI GGCC 1 cut(s) 483
BshNI GGYRCC 1 cut(s) 249
BshVI ATCGAT 1 cut(s) 78
BsiEI CGRYCG 1 cut(s) 278
BsiHKAI GWGCWC 2 cut(s) 714, 789
BsiSI CCGG 2 cut(s) 117, 284
BslFI GGGAC 1 cut(s) 201
BslI CCNNNNNNNGG 2 cut(s) 21, 490
BsmAI GTCTC 3 cut(s) 123, 348, 662
BsmBI CGTCTC 2 cut(s) 123, 348
BsmFI GGGAC 1 cut(s) 201
BsnI GGCC 1 cut(s) 483
Bso31I GGTCTC 1 cut(s) 662
Bsp1286I GDGCHC 2 cut(s) 714, 789
Bsp143I GATC 6 cut(s) 79, 216, 856, 862, 985, 1022
BspACI CCGC 5 cut(s) 36, 85, 88, 314, 625
BspANI GGCC 1 cut(s) 483
BspCNI CTCAG 1 cut(s) 45
BspDI ATCGAT 1 cut(s) 78
BspFNI CGCG 2 cut(s) 182, 681
BspHI TCATGA 1 cut(s) 988
BspLI GGNNCC 2 cut(s) 251, 637
BspMAI CTGCAG 2 cut(s) 427, 520
BspT107I GGYRCC 1 cut(s) 249
BspTNI GGTCTC 1 cut(s) 662
BsrDI GCAATG 1 cut(s) 541
BsrI ACTGG 2 cut(s) 484, 572
BssECI CCNNGG 1 cut(s) 284
BssMI GATC 6 cut(s) 79, 216, 856, 862, 985, 1022
Bst4CI ACNGT 3 cut(s) 107, 276, 475
BstAPI GCANNNNNTGC 1 cut(s) 793
BstC8I GCNNGC 2 cut(s) 124, 1031
BstDEI CTNAG 2 cut(s) 32, 201
BstF5I GGATG 3 cut(s) 294, 895, 967
BstFNI CGCG 2 cut(s) 182, 681
BstHHI GCGC 2 cut(s) 681, 871
BstKTI GATC 6 cut(s) 82, 219, 859, 865, 988, 1025
BstMAI GTCTC 3 cut(s) 123, 348, 662
BstMBI GATC 6 cut(s) 79, 216, 856, 862, 985, 1022
BstMCI CGRYCG 1 cut(s) 278
BstMWI GCNNNNNNNGC 6 cut(s) 422, 503, 506, 515, 793, 1035
BstNSI RCATGY 1 cut(s) 588
BstSCI CCNGG 1 cut(s) 283
BstSFI CTRYAG 3 cut(s) 423, 516, 558
BstSLI GKGCMC 1 cut(s) 789
BstUI CGCG 2 cut(s) 182, 681
BstV1I GCAGC 3 cut(s) 409, 484, 518
Bsu15I ATCGAT 1 cut(s) 78
BsuRI GGCC 1 cut(s) 483
BsuTUI ATCGAT 1 cut(s) 78
BtsCI GGATG 3 cut(s) 294, 895, 967
BtsIMutI CAGTG 2 cut(s) 477, 579
Cac8I GCNNGC 2 cut(s) 124, 1031
CciI TCATGA 1 cut(s) 988
CfoI GCGC 2 cut(s) 681, 871
Cfr13I GGNCC 2 cut(s) 114, 635
ClaI ATCGAT 1 cut(s) 78
CseI GACGC 2 cut(s) 140, 670
Csp6I GTAC 2 cut(s) 250, 434
CviAII CATG 4 cut(s) 490, 585, 809, 989
CviQI GTAC 2 cut(s) 250, 434
DdeI CTNAG 2 cut(s) 32, 201
DpnI GATC 6 cut(s) 81, 218, 858, 864, 987, 1024
DpnII GATC 6 cut(s) 79, 216, 856, 862, 985, 1022
EaeI YGGCCR 1 cut(s) 481
EciI GGCGGA 1 cut(s) 74
Eco31I GGTCTC 1 cut(s) 662
Eco47I GGWCC 2 cut(s) 114, 635
Eco57I CTGAAG 1 cut(s) 917
EcoO109I RGGNCCY 1 cut(s) 635
EcoT22I ATGCAT 1 cut(s) 649
Esp3I CGTCTC 2 cut(s) 123, 348
FaeI CATG 4 cut(s) 493, 588, 812, 992
FaqI GGGAC 1 cut(s) 201
FatI CATG 4 cut(s) 489, 584, 808, 988
FauI CCCGC 1 cut(s) 632
FblI GTMKAC 2 cut(s) 58, 522
Fnu4HI GCNGC 5 cut(s) 37, 88, 423, 498, 507
FokI GGATG 3 cut(s) 301, 902, 974
Fsp4HI GCNGC 5 cut(s) 37, 88, 423, 498, 507
FspBI CTAG 1 cut(s) 71
GlaI GCGC 2 cut(s) 680, 870
GluI GCNGC 5 cut(s) 37, 88, 423, 498, 507
GsaI CCCAGC 1 cut(s) 389
HaeIII GGCC 1 cut(s) 483
HapII CCGG 2 cut(s) 117, 284
HgaI GACGC 2 cut(s) 140, 670
HhaI GCGC 2 cut(s) 681, 871
Hin1II CATG 4 cut(s) 493, 588, 812, 992
Hin6I GCGC 2 cut(s) 679, 869
HinP1I GCGC 2 cut(s) 679, 869
HincII GTYRAC 1 cut(s) 981
HindII GTYRAC 1 cut(s) 981
HindIII AAGCTT 2 cut(s) 398, 1027
HpaI GTTAAC 1 cut(s) 981
HpaII CCGG 2 cut(s) 117, 284
HphI GGTGA 6 cut(s) 119, 253, 328, 455, 608, 747
Hpy166II GTNNAC 6 cut(s) 59, 523, 787, 877, 930, 981
Hpy188I TCNGA 2 cut(s) 718, 897
Hpy188III TCNNGA 4 cut(s) 71, 659, 860, 989
Hpy8I GTNNAC 6 cut(s) 59, 523, 787, 877, 930, 981
Hpy99I CGWCG 1 cut(s) 283
HpyAV CCTTC 5 cut(s) 178, 323, 827, 892, 1007
HpyCH4III ACNGT 3 cut(s) 107, 276, 475
HpyCH4IV ACGT 3 cut(s) 309, 357, 783
HpyF10VI GCNNNNNNNGC 6 cut(s) 422, 503, 506, 515, 793, 1035
HpyF3I CTNAG 2 cut(s) 32, 201
HpySE526I ACGT 3 cut(s) 309, 357, 783
Hsp92II CATG 4 cut(s) 493, 588, 812, 992
HspAI GCGC 2 cut(s) 679, 869
KpnI GGTACC 1 cut(s) 253
KspAI GTTAAC 1 cut(s) 981
Kzo9I GATC 6 cut(s) 79, 216, 856, 862, 985, 1022
LmnI GCTCC 2 cut(s) 119, 377
Lsp1109I GCAGC 3 cut(s) 409, 484, 518
LweI GCATC 1 cut(s) 955
MaeI CTAG 1 cut(s) 71
MaeII ACGT 3 cut(s) 309, 357, 783
MaeIII GTNAC 5 cut(s) 305, 316, 475, 592, 614
MalI GATC 6 cut(s) 81, 218, 858, 864, 987, 1024
MboI GATC 6 cut(s) 79, 216, 856, 862, 985, 1022
MboII GAAGA 2 cut(s) 187, 659
MhlI GDGCHC 2 cut(s) 714, 789
MlsI TGGCCA 1 cut(s) 483
MluCI AATT 1 cut(s) 799
MluNI TGGCCA 1 cut(s) 483
Mox20I TGGCCA 1 cut(s) 483
Mph1103I ATGCAT 1 cut(s) 649
MroXI GAANNNNTTC 1 cut(s) 666
MscI TGGCCA 1 cut(s) 483
MseI TTAA 1 cut(s) 980
MslI CAYNNNNRTG 2 cut(s) 105, 993
Msp20I TGGCCA 1 cut(s) 483
MspA1I CMGCKG 1 cut(s) 36
MspI CCGG 2 cut(s) 117, 284
MspR9I CCNGG 1 cut(s) 285
MvnI CGCG 2 cut(s) 182, 681
MwoI GCNNNNNNNGC 6 cut(s) 422, 503, 506, 515, 793, 1035
NciI CCSGG 1 cut(s) 285
NdeII GATC 6 cut(s) 79, 216, 856, 862, 985, 1022
NlaIII CATG 4 cut(s) 493, 588, 812, 992
NlaIV GGNNCC 2 cut(s) 251, 637
NmuCI GTSAC 4 cut(s) 316, 475, 592, 614
NsiI ATGCAT 1 cut(s) 649
NspI RCATGY 1 cut(s) 588
OliI CACNNNNGTG 1 cut(s) 105
PagI TCATGA 1 cut(s) 988
PdmI GAANNNNTTC 1 cut(s) 666
PflFI GACNNNGTC 1 cut(s) 134
PflMI CCANNNNNTGG 2 cut(s) 21, 490
PkrI GCNGC 5 cut(s) 38, 89, 424, 499, 508
PpuMI RGGWCCY 1 cut(s) 635
Psp5II RGGWCCY 1 cut(s) 635
PspFI CCCAGC 1 cut(s) 385
PspN4I GGNNCC 2 cut(s) 251, 637
PspPI GGNCC 2 cut(s) 114, 635
PspPPI RGGWCCY 1 cut(s) 635
PsrI GAACNNNNNNTAC 2 cut(s) 513, 545
PstI CTGCAG 2 cut(s) 427, 520
PsyI GACNNNGTC 1 cut(s) 134
RsaI GTAC 2 cut(s) 251, 435
RsaNI GTAC 2 cut(s) 250, 434
RseI CAYNNNNRTG 2 cut(s) 105, 993
SaqAI TTAA 1 cut(s) 980
SatI GCNGC 5 cut(s) 37, 88, 423, 498, 507
Sau3AI GATC 6 cut(s) 79, 216, 856, 862, 985, 1022
Sau96I GGNCC 2 cut(s) 114, 635
ScaI AGTACT 1 cut(s) 435
ScrFI CCNGG 1 cut(s) 285
SduI GDGCHC 2 cut(s) 714, 789
SfaNI GCATC 1 cut(s) 955
SfcI CTRYAG 3 cut(s) 423, 516, 558
SinI GGWCC 2 cut(s) 114, 635
SmiMI CAYNNNNRTG 2 cut(s) 105, 993
SmlI CTYRAG 1 cut(s) 417
SmoI CTYRAG 1 cut(s) 417
Sse9I AATT 1 cut(s) 799
SsiI CCGC 5 cut(s) 36, 85, 88, 314, 625
SspMI CTAG 1 cut(s) 71
StyD4I CCNGG 1 cut(s) 283
TaaI ACNGT 3 cut(s) 107, 276, 475
TaiI ACGT 3 cut(s) 312, 360, 786
TaqI TCGA 4 cut(s) 78, 660, 741, 849
TasI AATT 1 cut(s) 799
TatI WGTACW 1 cut(s) 433
TauI GCSGC 2 cut(s) 39, 90
Tru1I TTAA 1 cut(s) 980
Tru9I TTAA 1 cut(s) 980
TscAI CASTG 2 cut(s) 484, 579
TseFI GTSAC 4 cut(s) 316, 475, 592, 614
TseI GCWGC 3 cut(s) 422, 497, 506
Tsp45I GTSAC 4 cut(s) 316, 475, 592, 614
TspDTI ATGAA 3 cut(s) 342, 593, 1026
TspGWI ACGGA 1 cut(s) 180
TspRI CASTG 2 cut(s) 484, 579
Tth111I GACNNNGTC 1 cut(s) 134
Van91I CCANNNNNTGG 2 cut(s) 21, 490
VneI GTGCAC 1 cut(s) 785
VpaK11BI GGWCC 2 cut(s) 114, 635
XbaI TCTAGA 1 cut(s) 70
XceI RCATGY 1 cut(s) 588
XmiI GTMKAC 2 cut(s) 58, 522
XmnI GAANNNNTTC 1 cut(s) 666
XspI CTAG 1 cut(s) 71
ZrmI AGTACT 1 cut(s) 435
Zsp2I ATGCAT 1 cut(s) 649
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.