Rh2CG288700

phospholipase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr2C
OG0017969
Physical Location & Seq
Reverse (-)
32489764 .. 32491192
1429 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh2CG288700.1

Sequence Viewer

Length: 1116 bp
ATGGGCAGCATAGCCACAAGATGGAGGCAACTGAGCGGCGAGAAGGATTGGGAGGGTCTATTGTACCCTCTGGACATTGATCTCCGCCGCTACATAATCCACTATGGTGAAAGGGCCGGAGCTAGTAGAGACGCTGTCATTGCTGAGCCAAAATCAAAGAACTTTGGACAACCCAGATATGCAAAGCGACATTTATTCTCTAAGGTGGGTTTGGAGATCGGGAATCCATACAAATATGTGGTGAGAAAGTATTTATATGCAGCACCAAAACTACGGTCGTCCCCGGGAATGTCTTGTTTGTTGGGTTACGTTGCGGTGACAACAGATGAAGGAAGCAAAGTGTTGGGGAGGAGGGACGTTCTCATTTCGTTTAGAGGAGCAGTGCTGGGACATGAGAAGCTTGGTCATCCTACAGTGCACCTTCCTTGGCATACCTATTACACTAATGTTGACCTTCAATCCCCACACAACAAAACTAGCTGCAGAGATCAGGCTCAAGTTGCAGTAAGAGAACTATTGGGTCAATACAAGGACGAGGAAATCAGCGTTACTGCCACTGGCCATAGCATGGGTGCTGCATTCGCAGCTCAAACTGCAATAGACCTCGTTCACAATGGATTCAATGTCATAAAACCTACAGGAATACCAGTGAAAGCATGTCTTGTCACAGCCATCCTGTTTGCCTGCCCCCCTCGCCGCATTTTGGGATCCCAAAATGTAATTTCAGGAGGTCTAGAATATCTTCACATCTTGCGTGTCCAAAACAAATATGATAACCATAATGAGCACCAAGATGTTGGTATAGAGTTCAAAATCGACACTTTGAAATCACCATACTTGAAGAATGCACTACAAAACGTGCACGACTTGCAGAATTACTTGCATGGAGTTGCAGGAAATCAAGGGGAAAGTGGTTTTAAGCTCGATGTTAATCGTGATCTCGCGCTAGTAAACAAATACGTGGATGGTCTCAAGGATGAATATAATGTAGTTGCGAAGTGGTGGACAGAGAAGAACAAGTCTATGGTCCAAATGGATGATGGCTCTTGGGTGTTGATAGATCTTGAGAATGATGGTGATGAAGGAAACAAATTAAGCTTGCAAAGCCGAATTTAA

Protein Analysis

371

Amino Acids

41.57

Weight (kDa)

8.65

Isoelectric Point (pI)

45.83

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Lipase_3 PF01764 136 - 230 9.2e-10 Lipase (class 3)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0017969)

Species Orthologous Gene IDs
fragaria_vesca FvH4_1g25210
rosa_chinensis RchiOBHm_Chr2g0122911
rosa_laevigata RLG00000018725
rosa_multiflora Rmu_sc0001635.1_g000003
rosa_roxburghii Rroxscaffold_2G00120960
rosa_rugosa Rorug02G0243000
rosa_samantha Rh2AG302100 Rh2BG309600 Rh2CG288700 Rh2DG326100
rosa_wichuraiana Rw2G024000

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB7I CCANNNNNTGG 2 cut(s) 21, 568
AccBSI CCGCTC 1 cut(s) 36
AccII CGCG 1 cut(s) 944
AciI CCGC 5 cut(s) 36, 85, 88, 314, 697
AclWI GGATC 2 cut(s) 702, 715
AcoI YGGCCR 1 cut(s) 559
AcsI RAATTY 1 cut(s) 1110
AfaI GTAC 1 cut(s) 65
AfiI CCNNNNNNNGG 3 cut(s) 21, 568, 703
AgsI TTSAA 5 cut(s) 458, 622, 811, 826, 841
AleI CACNNNNGTG 1 cut(s) 105
AluBI AGCT 6 cut(s) 122, 400, 480, 587, 922, 1098
AluI AGCT 6 cut(s) 122, 400, 480, 587, 922, 1098
Alw21I GWGCWC 3 cut(s) 420, 789, 864
Alw26I GTCTC 2 cut(s) 123, 974
Alw44I GTGCAC 2 cut(s) 416, 860
AlwI GGATC 2 cut(s) 702, 715
Ama87I CYCGRG 1 cut(s) 283
AoxI GGCC 2 cut(s) 114, 559
ApaLI GTGCAC 2 cut(s) 416, 860
ApeKI GCWGC 5 cut(s) 6, 260, 480, 575, 584
ApoI RAATTY 1 cut(s) 1110
ArsI GACNNNNNNTTYG 2 cut(s) 260, 292
Asp700I GAANNNNTTC 1 cut(s) 741
AspLEI GCGC 1 cut(s) 946
AspS9I GGNCC 2 cut(s) 114, 1027
AsuC2I CCSGG 2 cut(s) 284, 285
AsuHPI GGTGA 5 cut(s) 119, 253, 328, 822, 1088
AvaI CYCGRG 1 cut(s) 283
AvaII GGWCC 1 cut(s) 1027
BaeGI GKGCMC 2 cut(s) 420, 864
BalI TGGCCA 1 cut(s) 561
BamHI GGATCC 1 cut(s) 707
Bbv12I GWGCWC 3 cut(s) 420, 789, 864
BbvI GCAGC 5 cut(s) 18, 272, 467, 562, 596
BccI CCATC 5 cut(s) 15, 680, 959, 1034, 1067
BcnI CCSGG 2 cut(s) 284, 285
BcoDI GTCTC 2 cut(s) 123, 974
BfaI CTAG 4 cut(s) 123, 477, 734, 947
BfmI CTRYAG 3 cut(s) 411, 481, 636
BglII AGATCT 1 cut(s) 1060
BisI GCNGC 8 cut(s) 7, 37, 88, 261, 481, 576, 585, 697
BlpI GCTNAGC 1 cut(s) 144
BlsI GCNGC 8 cut(s) 8, 38, 89, 262, 482, 577, 586, 698
Bme1390I CCNGG 2 cut(s) 284, 285
Bme18I GGWCC 1 cut(s) 1027
BmeT110I CYCGRG 1 cut(s) 283
BmgT120I GGNCC 2 cut(s) 114, 1027
BmiI GGNNCC 1 cut(s) 709
BmrFI CCNGG 2 cut(s) 284, 285
Bpu1102I GCTNAGC 1 cut(s) 144
BpuEI CTTGAG 3 cut(s) 480, 956, 1085
BpuMI CCSGG 2 cut(s) 284, 285
BsaAI YACGTR 1 cut(s) 961
BsaBI GATNNNNATC 1 cut(s) 930
BsaI GGTCTC 1 cut(s) 974
BsaJI CCNNGG 3 cut(s) 282, 283, 425
Bsc4I CCNNNNNNNGG 3 cut(s) 21, 568, 703
Bse1I ACTGG 2 cut(s) 562, 647
Bse3DI GCAATG 1 cut(s) 138
Bse8I GATNNNNATC 1 cut(s) 930
BseDI CCNNGG 3 cut(s) 282, 283, 425
BseGI GGATG 5 cut(s) 406, 672, 970, 982, 1042
BseJI GATNNNNATC 1 cut(s) 930
BseLI CCNNNNNNNGG 3 cut(s) 21, 568, 703
BseMI GCAATG 1 cut(s) 138
BseMII CTCAG 2 cut(s) 23, 135
BseNI ACTGG 2 cut(s) 562, 647
BseRI GAGGAG 2 cut(s) 364, 390
BseSI GKGCMC 2 cut(s) 420, 864
BseXI GCAGC 5 cut(s) 18, 272, 467, 562, 596
BseYI CCCAGC 1 cut(s) 385
Bsh1236I CGCG 1 cut(s) 944
Bsh1285I CGRYCG 1 cut(s) 278
BshFI GGCC 2 cut(s) 116, 561
BsiEI CGRYCG 1 cut(s) 278
BsiHKAI GWGCWC 3 cut(s) 420, 789, 864
BsiHKCI CYCGRG 1 cut(s) 283
BsiSI CCGG 2 cut(s) 117, 284
BslFI GGGAC 3 cut(s) 265, 368, 402
BslI CCNNNNNNNGG 3 cut(s) 21, 568, 703
BsmAI GTCTC 2 cut(s) 123, 974
BsmBI CGTCTC 1 cut(s) 123
BsmFI GGGAC 3 cut(s) 265, 368, 402
BsmI GAATGC 2 cut(s) 578, 850
BsnI GGCC 2 cut(s) 116, 561
Bso31I GGTCTC 1 cut(s) 974
BsoBI CYCGRG 1 cut(s) 283
Bsp1286I GDGCHC 3 cut(s) 420, 789, 864
Bsp143I GATC 6 cut(s) 79, 216, 487, 707, 937, 1060
Bsp1720I GCTNAGC 1 cut(s) 144
BspACI CCGC 5 cut(s) 36, 85, 88, 314, 697
BspANI GGCC 2 cut(s) 116, 561
BspCNI CTCAG 2 cut(s) 24, 136
BspFNI CGCG 1 cut(s) 944
BspLI GGNNCC 1 cut(s) 709
BspMAI CTGCAG 1 cut(s) 485
BspPI GGATC 2 cut(s) 702, 715
BspTNI GGTCTC 1 cut(s) 974
BsrBI CCGCTC 1 cut(s) 36
BsrDI GCAATG 1 cut(s) 138
BsrI ACTGG 2 cut(s) 562, 647
BssECI CCNNGG 3 cut(s) 282, 283, 425
BssMI GATC 6 cut(s) 79, 216, 487, 707, 937, 1060
BssT1I CCWWGG 1 cut(s) 425
Bst4CI ACNGT 2 cut(s) 276, 415
BstAPI GCANNNNNTGC 1 cut(s) 868
BstBAI YACGTR 1 cut(s) 961
BstC8I GCNNGC 2 cut(s) 685, 1100
BstDEI CTNAG 3 cut(s) 32, 144, 201
BstF5I GGATG 5 cut(s) 406, 672, 970, 982, 1042
BstFNI CGCG 1 cut(s) 944
BstHHI GCGC 1 cut(s) 946
BstKTI GATC 6 cut(s) 82, 219, 490, 710, 940, 1063
BstMAI GTCTC 2 cut(s) 123, 974
BstMBI GATC 6 cut(s) 79, 216, 487, 707, 937, 1060
BstMCI CGRYCG 1 cut(s) 278
BstMWI GCNNNNNNNGC 8 cut(s) 140, 500, 581, 584, 593, 693, 868, 1104
BstNSI RCATGY 1 cut(s) 660
BstSCI CCNGG 2 cut(s) 282, 283
BstSFI CTRYAG 3 cut(s) 411, 481, 636
BstSLI GKGCMC 2 cut(s) 420, 864
BstUI CGCG 1 cut(s) 944
BstV1I GCAGC 5 cut(s) 18, 272, 467, 562, 596
BstX2I RGATCY 2 cut(s) 707, 1060
BstXI CCANNNNNNTGG 1 cut(s) 797
BstYI RGATCY 2 cut(s) 707, 1060
BsuRI GGCC 2 cut(s) 116, 561
BtsCI GGATG 5 cut(s) 406, 672, 970, 982, 1042
BtsI GCAGTG 1 cut(s) 387
BtsIMutI CAGTG 4 cut(s) 387, 420, 555, 654
Cac8I GCNNGC 2 cut(s) 685, 1100
CfoI GCGC 1 cut(s) 946
Cfr13I GGNCC 2 cut(s) 114, 1027
Cfr9I CCCGGG 1 cut(s) 283
CseI GACGC 1 cut(s) 140
Csp6I GTAC 1 cut(s) 64
CviAII CATG 4 cut(s) 392, 568, 657, 884
CviQI GTAC 1 cut(s) 64
DdeI CTNAG 3 cut(s) 32, 144, 201
DpnI GATC 6 cut(s) 81, 218, 489, 709, 939, 1062
DpnII GATC 6 cut(s) 79, 216, 487, 707, 937, 1060
EaeI YGGCCR 1 cut(s) 559
EciI GGCGGA 1 cut(s) 74
Eco130I CCWWGG 1 cut(s) 425
Eco31I GGTCTC 1 cut(s) 974
Eco47I GGWCC 1 cut(s) 1027
Eco88I CYCGRG 1 cut(s) 283
EcoT14I CCWWGG 1 cut(s) 425
ErhI CCWWGG 1 cut(s) 425
Esp3I CGTCTC 1 cut(s) 123
FaeI CATG 4 cut(s) 395, 571, 660, 887
FalI AAGNNNNNCTT 2 cut(s) 645, 677
FaqI GGGAC 3 cut(s) 265, 368, 402
FatI CATG 4 cut(s) 391, 567, 656, 883
Fnu4HI GCNGC 8 cut(s) 7, 37, 88, 261, 481, 576, 585, 697
FokI GGATG 5 cut(s) 393, 659, 977, 989, 1049
Fsp4HI GCNGC 8 cut(s) 7, 37, 88, 261, 481, 576, 585, 697
FspBI CTAG 4 cut(s) 123, 477, 734, 947
GlaI GCGC 1 cut(s) 945
GluI GCNGC 8 cut(s) 7, 37, 88, 261, 481, 576, 585, 697
GsaI CCCAGC 1 cut(s) 389
HaeIII GGCC 2 cut(s) 116, 561
HapII CCGG 2 cut(s) 117, 284
HgaI GACGC 1 cut(s) 140
HhaI GCGC 1 cut(s) 946
Hin1II CATG 4 cut(s) 395, 571, 660, 887
Hin6I GCGC 1 cut(s) 944
HinP1I GCGC 1 cut(s) 944
HincII GTYRAC 1 cut(s) 451
HindII GTYRAC 1 cut(s) 451
HindIII AAGCTT 2 cut(s) 398, 1096
HinfI GANTC 2 cut(s) 223, 618
HpaII CCGG 2 cut(s) 117, 284
HphI GGTGA 5 cut(s) 119, 253, 328, 822, 1088
Hpy166II GTNNAC 6 cut(s) 418, 451, 610, 862, 952, 1005
Hpy188III TCNNGA 6 cut(s) 71, 220, 726, 734, 935, 1064
Hpy8I GTNNAC 6 cut(s) 418, 451, 610, 862, 952, 1005
HpyAV CCTTC 5 cut(s) 37, 323, 431, 464, 1076
HpyCH4III ACNGT 2 cut(s) 276, 415
HpyCH4IV ACGT 4 cut(s) 309, 357, 858, 960
HpyF10VI GCNNNNNNNGC 8 cut(s) 140, 500, 581, 584, 593, 693, 868, 1104
HpyF3I CTNAG 3 cut(s) 32, 144, 201
HpySE526I ACGT 4 cut(s) 309, 357, 858, 960
Hsp92II CATG 4 cut(s) 395, 571, 660, 887
HspAI GCGC 1 cut(s) 944
Kzo9I GATC 6 cut(s) 79, 216, 487, 707, 937, 1060
LmnI GCTCC 2 cut(s) 119, 377
Lsp1109I GCAGC 5 cut(s) 18, 272, 467, 562, 596
MaeI CTAG 4 cut(s) 123, 477, 734, 947
MaeII ACGT 4 cut(s) 309, 357, 858, 960
MaeIII GTNAC 4 cut(s) 305, 316, 547, 664
MalI GATC 6 cut(s) 81, 218, 489, 709, 939, 1062
MbiI CCGCTC 1 cut(s) 36
MboI GATC 6 cut(s) 79, 216, 487, 707, 937, 1060
MboII GAAGA 3 cut(s) 734, 853, 1024
MflI RGATCY 2 cut(s) 707, 1060
MhlI GDGCHC 3 cut(s) 420, 789, 864
MlsI TGGCCA 1 cut(s) 561
MluCI AATT 4 cut(s) 720, 874, 1091, 1110
MluNI TGGCCA 1 cut(s) 561
Mox20I TGGCCA 1 cut(s) 561
MroXI GAANNNNTTC 1 cut(s) 741
MscI TGGCCA 1 cut(s) 561
MseI TTAA 4 cut(s) 918, 930, 1094, 1114
MslI CAYNNNNRTG 2 cut(s) 105, 792
Msp20I TGGCCA 1 cut(s) 561
MspI CCGG 2 cut(s) 117, 284
MspR9I CCNGG 2 cut(s) 284, 285
Mva1269I GAATGC 2 cut(s) 578, 850
MvnI CGCG 1 cut(s) 944
MwoI GCNNNNNNNGC 8 cut(s) 140, 500, 581, 584, 593, 693, 868, 1104
NciI CCSGG 2 cut(s) 284, 285
NdeII GATC 6 cut(s) 79, 216, 487, 707, 937, 1060
NlaIII CATG 4 cut(s) 395, 571, 660, 887
NlaIV GGNNCC 1 cut(s) 709
NmuCI GTSAC 2 cut(s) 316, 664
NspI RCATGY 1 cut(s) 660
OliI CACNNNNGTG 1 cut(s) 105
PctI GAATGC 2 cut(s) 578, 850
PdmI GAANNNNTTC 1 cut(s) 741
PfeI GAWTC 2 cut(s) 223, 618
PflFI GACNNNGTC 1 cut(s) 134
PflMI CCANNNNNTGG 2 cut(s) 21, 568
PkrI GCNGC 8 cut(s) 8, 38, 89, 262, 482, 577, 586, 698
Ppu21I YACGTR 1 cut(s) 961
PspFI CCCAGC 1 cut(s) 385
PspN4I GGNNCC 1 cut(s) 709
PspPI GGNCC 2 cut(s) 114, 1027
PstI CTGCAG 1 cut(s) 485
PsuI RGATCY 2 cut(s) 707, 1060
PsyI GACNNNGTC 1 cut(s) 134
RsaI GTAC 1 cut(s) 65
RsaNI GTAC 1 cut(s) 64
RseI CAYNNNNRTG 2 cut(s) 105, 792
SaqAI TTAA 4 cut(s) 918, 930, 1094, 1114
SatI GCNGC 8 cut(s) 7, 37, 88, 261, 481, 576, 585, 697
Sau3AI GATC 6 cut(s) 79, 216, 487, 707, 937, 1060
Sau96I GGNCC 2 cut(s) 114, 1027
ScrFI CCNGG 2 cut(s) 284, 285
SduI GDGCHC 3 cut(s) 420, 789, 864
SfcI CTRYAG 3 cut(s) 411, 481, 636
SinI GGWCC 1 cut(s) 1027
SmaI CCCGGG 1 cut(s) 285
SmiMI CAYNNNNRTG 2 cut(s) 105, 792
SmlI CTYRAG 3 cut(s) 495, 971, 1064
SmoI CTYRAG 3 cut(s) 495, 971, 1064
Sse9I AATT 4 cut(s) 720, 874, 1091, 1110
SsiI CCGC 5 cut(s) 36, 85, 88, 314, 697
SspMI CTAG 4 cut(s) 123, 477, 734, 947
StyD4I CCNGG 2 cut(s) 282, 283
StyI CCWWGG 1 cut(s) 425
TaaI ACNGT 2 cut(s) 276, 415
TaiI ACGT 4 cut(s) 312, 360, 861, 963
TaqI TCGA 2 cut(s) 816, 924
TasI AATT 4 cut(s) 720, 874, 1091, 1110
TauI GCSGC 3 cut(s) 39, 90, 699
TfiI GAWTC 2 cut(s) 223, 618
Tru1I TTAA 4 cut(s) 918, 930, 1094, 1114
Tru9I TTAA 4 cut(s) 918, 930, 1094, 1114
TscAI CASTG 4 cut(s) 387, 420, 562, 654
TseFI GTSAC 2 cut(s) 316, 664
TseI GCWGC 5 cut(s) 6, 260, 480, 575, 584
Tsp45I GTSAC 2 cut(s) 316, 664
TspDTI ATGAA 3 cut(s) 342, 993, 1095
TspMI CCCGGG 1 cut(s) 283
TspRI CASTG 4 cut(s) 387, 420, 562, 654
Tth111I GACNNNGTC 1 cut(s) 134
Van91I CCANNNNNTGG 2 cut(s) 21, 568
VneI GTGCAC 2 cut(s) 416, 860
VpaK11BI GGWCC 1 cut(s) 1027
XapI RAATTY 1 cut(s) 1110
XbaI TCTAGA 1 cut(s) 733
XceI RCATGY 1 cut(s) 660
XcmI CCANNNNNNNNNTGG 1 cut(s) 1037
XmaI CCCGGG 1 cut(s) 283
XmnI GAANNNNTTC 1 cut(s) 741
XspI CTAG 4 cut(s) 123, 477, 734, 947
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.