Rh2DG326100

phospholipase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr2D
OG0017969
Physical Location & Seq
Reverse (-)
41073656 .. 41075113
1458 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh2DG326100.1

Sequence Viewer

Length: 1113 bp
ATGGGCATAGCCACAAGATGGAGGCAACTGAGCGGCGAGAAGGATTGGGAGGGTCTATTGTACCCTCTGGACATTGATCTCCGCCGCTACATAATCCACTATGGTGAAAGGGCCGGAGCTAGTAGAGACGCTGTCATTGCTGAGCCAAAATCAAAGAACTTTGGACAACCCAGATATGCAAAGCGACATTTATTCTCTAAGGTGGGTTTGGAGATCGGGAATCCATACAAATATGTGGTGAGAAAGTATTTATATGCAGCACCAAAACTACCGTCGTCCCCGGGAATGTCTTGTTTGTTGGGTTACGTTGCGGTGACAACAGATGAAGGAAGCAAAGTGTTGGGGAGGAGGGACGTTCTCATTTCGTTTAGAGGAGCAGTGCTGGGACATGAGAAGCTTGGTCATCCTACAGTGCACCTTCCTTGGCATACCTATTACACTAATGTTGACCTTCAATCCCCACACAACAAAACTAGCTGCAGAGATCAGGCTCAAGCTGCAGTAAGAGAACTATTGGGTCAATACAAGGACGAGGAAATCAGCGTTACTGCCACTGGCCATAGCATGGGTGCTGCATTCGCAGCTCAAACTGCAATAGACCTCGTTCACAATGGATTCAATATCACAAAACCTACAGGAATACCAGTGAAAGAATGTCTTGTCACAGCCATCCTGTTTGCCTGCCCCCCTCGCCGCATTTTGGGATCCCAAAATGTAATTTCAGGAGGTCTAGAATATCTTCACATCTTGCGTGTCCAAAACAAATATGATAACCATAATGAGCACCAAGATGTTGGTATAGAGTTCAAAATCGACACTTTGAAATCACCATACTTGAAGAATGCACTACAAAACGTGCACGACTTGCAGAATTACTTGCATGGAGTTGCAGGAAATCAAGGGGAAAGTGGTTTTAAGCTCGATGTTAATCGTGATCTCGCGCTAGTAAACAAATACGTGGATGGTCTCAAGGATGAATATAATGTAGTTGCGAAGTGGTGGACAGAGAAGAACAAGTCTATGGTCCAAATGGATGATGGCTCTTGGGTGTTGATAGATCATGAGAATGATGGTGATGAAGGAAACAAATTAAGCTTGCAAAGCCGAATTTAA

Protein Analysis

370

Amino Acids

41.48

Weight (kDa)

8.18

Isoelectric Point (pI)

46.65

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Lipase_3 PF01764 135 - 229 3.4e-10 Lipase (class 3)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0017969)

Species Orthologous Gene IDs
fragaria_vesca FvH4_1g25210
rosa_chinensis RchiOBHm_Chr2g0122911
rosa_laevigata RLG00000018725
rosa_multiflora Rmu_sc0001635.1_g000003
rosa_roxburghii Rroxscaffold_2G00120960
rosa_rugosa Rorug02G0243000
rosa_samantha Rh2AG302100 Rh2BG309600 Rh2CG288700 Rh2DG326100
rosa_wichuraiana Rw2G024000

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB7I CCANNNNNTGG 2 cut(s) 18, 565
AccBSI CCGCTC 1 cut(s) 33
AccII CGCG 1 cut(s) 941
AciI CCGC 5 cut(s) 33, 82, 85, 311, 694
AclWI GGATC 2 cut(s) 699, 712
AcoI YGGCCR 1 cut(s) 556
AcsI RAATTY 1 cut(s) 1107
AfaI GTAC 1 cut(s) 62
AfiI CCNNNNNNNGG 3 cut(s) 18, 565, 700
AgsI TTSAA 5 cut(s) 455, 619, 808, 823, 838
AleI CACNNNNGTG 1 cut(s) 102
AluBI AGCT 7 cut(s) 119, 397, 477, 497, 584, 919, 1095
AluI AGCT 7 cut(s) 119, 397, 477, 497, 584, 919, 1095
Alw21I GWGCWC 3 cut(s) 417, 786, 861
Alw26I GTCTC 2 cut(s) 120, 971
Alw44I GTGCAC 2 cut(s) 413, 857
AlwI GGATC 2 cut(s) 699, 712
Ama87I CYCGRG 1 cut(s) 280
AoxI GGCC 2 cut(s) 111, 556
ApaLI GTGCAC 2 cut(s) 413, 857
ApeKI GCWGC 5 cut(s) 257, 477, 497, 572, 581
ApoI RAATTY 1 cut(s) 1107
ArsI GACNNNNNNTTYG 2 cut(s) 257, 289
Asp700I GAANNNNTTC 1 cut(s) 738
AspLEI GCGC 1 cut(s) 943
AspS9I GGNCC 2 cut(s) 111, 1024
AsuC2I CCSGG 2 cut(s) 281, 282
AsuHPI GGTGA 5 cut(s) 116, 250, 325, 819, 1085
AvaI CYCGRG 1 cut(s) 280
AvaII GGWCC 1 cut(s) 1024
BaeGI GKGCMC 2 cut(s) 417, 861
BalI TGGCCA 1 cut(s) 558
BamHI GGATCC 1 cut(s) 704
Bbv12I GWGCWC 3 cut(s) 417, 786, 861
BbvI GCAGC 5 cut(s) 269, 464, 484, 559, 593
BccI CCATC 5 cut(s) 12, 677, 956, 1031, 1064
BcnI CCSGG 2 cut(s) 281, 282
BcoDI GTCTC 2 cut(s) 120, 971
BfaI CTAG 4 cut(s) 120, 474, 731, 944
BfmI CTRYAG 4 cut(s) 408, 478, 498, 633
BisI GCNGC 8 cut(s) 34, 85, 258, 478, 498, 573, 582, 694
BlpI GCTNAGC 1 cut(s) 141
BlsI GCNGC 8 cut(s) 35, 86, 259, 479, 499, 574, 583, 695
Bme1390I CCNGG 2 cut(s) 281, 282
Bme18I GGWCC 1 cut(s) 1024
BmeT110I CYCGRG 1 cut(s) 280
BmgT120I GGNCC 2 cut(s) 111, 1024
BmiI GGNNCC 1 cut(s) 706
BmrFI CCNGG 2 cut(s) 281, 282
Bpu1102I GCTNAGC 1 cut(s) 141
BpuEI CTTGAG 2 cut(s) 477, 953
BpuMI CCSGG 2 cut(s) 281, 282
BsaAI YACGTR 1 cut(s) 958
BsaBI GATNNNNATC 1 cut(s) 927
BsaI GGTCTC 1 cut(s) 971
BsaJI CCNNGG 3 cut(s) 279, 280, 422
Bsc4I CCNNNNNNNGG 3 cut(s) 18, 565, 700
Bse1I ACTGG 2 cut(s) 559, 644
Bse3DI GCAATG 1 cut(s) 135
Bse8I GATNNNNATC 1 cut(s) 927
BseDI CCNNGG 3 cut(s) 279, 280, 422
BseGI GGATG 5 cut(s) 403, 669, 967, 979, 1039
BseJI GATNNNNATC 1 cut(s) 927
BseLI CCNNNNNNNGG 3 cut(s) 18, 565, 700
BseMI GCAATG 1 cut(s) 135
BseMII CTCAG 2 cut(s) 20, 132
BseNI ACTGG 2 cut(s) 559, 644
BseRI GAGGAG 2 cut(s) 361, 387
BseSI GKGCMC 2 cut(s) 417, 861
BseXI GCAGC 5 cut(s) 269, 464, 484, 559, 593
BseYI CCCAGC 1 cut(s) 382
Bsh1236I CGCG 1 cut(s) 941
BshFI GGCC 2 cut(s) 113, 558
BsiHKAI GWGCWC 3 cut(s) 417, 786, 861
BsiHKCI CYCGRG 1 cut(s) 280
BsiSI CCGG 2 cut(s) 114, 281
BslFI GGGAC 3 cut(s) 262, 365, 399
BslI CCNNNNNNNGG 3 cut(s) 18, 565, 700
BsmAI GTCTC 2 cut(s) 120, 971
BsmBI CGTCTC 1 cut(s) 120
BsmFI GGGAC 3 cut(s) 262, 365, 399
BsmI GAATGC 2 cut(s) 575, 847
BsnI GGCC 2 cut(s) 113, 558
Bso31I GGTCTC 1 cut(s) 971
BsoBI CYCGRG 1 cut(s) 280
Bsp1286I GDGCHC 3 cut(s) 417, 786, 861
Bsp143I GATC 6 cut(s) 76, 213, 484, 704, 934, 1057
Bsp1720I GCTNAGC 1 cut(s) 141
BspACI CCGC 5 cut(s) 33, 82, 85, 311, 694
BspANI GGCC 2 cut(s) 113, 558
BspCNI CTCAG 2 cut(s) 21, 133
BspFNI CGCG 1 cut(s) 941
BspHI TCATGA 1 cut(s) 1060
BspLI GGNNCC 1 cut(s) 706
BspMAI CTGCAG 2 cut(s) 482, 502
BspPI GGATC 2 cut(s) 699, 712
BspTNI GGTCTC 1 cut(s) 971
BsrBI CCGCTC 1 cut(s) 33
BsrDI GCAATG 1 cut(s) 135
BsrI ACTGG 2 cut(s) 559, 644
BssECI CCNNGG 3 cut(s) 279, 280, 422
BssMI GATC 6 cut(s) 76, 213, 484, 704, 934, 1057
BssT1I CCWWGG 1 cut(s) 422
Bst4CI ACNGT 2 cut(s) 273, 412
BstAPI GCANNNNNTGC 1 cut(s) 865
BstBAI YACGTR 1 cut(s) 958
BstC8I GCNNGC 2 cut(s) 682, 1097
BstDEI CTNAG 3 cut(s) 29, 141, 198
BstF5I GGATG 5 cut(s) 403, 669, 967, 979, 1039
BstFNI CGCG 1 cut(s) 941
BstHHI GCGC 1 cut(s) 943
BstKTI GATC 6 cut(s) 79, 216, 487, 707, 937, 1060
BstMAI GTCTC 2 cut(s) 120, 971
BstMBI GATC 6 cut(s) 76, 213, 484, 704, 934, 1057
BstMWI GCNNNNNNNGC 8 cut(s) 137, 497, 578, 581, 590, 690, 865, 1101
BstSCI CCNGG 2 cut(s) 279, 280
BstSFI CTRYAG 4 cut(s) 408, 478, 498, 633
BstSLI GKGCMC 2 cut(s) 417, 861
BstUI CGCG 1 cut(s) 941
BstV1I GCAGC 5 cut(s) 269, 464, 484, 559, 593
BstX2I RGATCY 1 cut(s) 704
BstXI CCANNNNNNTGG 1 cut(s) 794
BstYI RGATCY 1 cut(s) 704
BsuRI GGCC 2 cut(s) 113, 558
BtsCI GGATG 5 cut(s) 403, 669, 967, 979, 1039
BtsI GCAGTG 1 cut(s) 384
BtsIMutI CAGTG 4 cut(s) 384, 417, 552, 651
Cac8I GCNNGC 2 cut(s) 682, 1097
CciI TCATGA 1 cut(s) 1060
CfoI GCGC 1 cut(s) 943
Cfr13I GGNCC 2 cut(s) 111, 1024
Cfr9I CCCGGG 1 cut(s) 280
CseI GACGC 1 cut(s) 137
Csp6I GTAC 1 cut(s) 61
CviAII CATG 4 cut(s) 389, 565, 881, 1061
CviQI GTAC 1 cut(s) 61
DdeI CTNAG 3 cut(s) 29, 141, 198
DpnI GATC 6 cut(s) 78, 215, 486, 706, 936, 1059
DpnII GATC 6 cut(s) 76, 213, 484, 704, 934, 1057
EaeI YGGCCR 1 cut(s) 556
EciI GGCGGA 1 cut(s) 71
Eco130I CCWWGG 1 cut(s) 422
Eco31I GGTCTC 1 cut(s) 971
Eco47I GGWCC 1 cut(s) 1024
Eco88I CYCGRG 1 cut(s) 280
EcoT14I CCWWGG 1 cut(s) 422
ErhI CCWWGG 1 cut(s) 422
Esp3I CGTCTC 1 cut(s) 120
FaeI CATG 4 cut(s) 392, 568, 884, 1064
FalI AAGNNNNNCTT 2 cut(s) 642, 674
FaqI GGGAC 3 cut(s) 262, 365, 399
FatI CATG 4 cut(s) 388, 564, 880, 1060
Fnu4HI GCNGC 8 cut(s) 34, 85, 258, 478, 498, 573, 582, 694
FokI GGATG 5 cut(s) 390, 656, 974, 986, 1046
Fsp4HI GCNGC 8 cut(s) 34, 85, 258, 478, 498, 573, 582, 694
FspBI CTAG 4 cut(s) 120, 474, 731, 944
GlaI GCGC 1 cut(s) 942
GluI GCNGC 8 cut(s) 34, 85, 258, 478, 498, 573, 582, 694
GsaI CCCAGC 1 cut(s) 386
HaeIII GGCC 2 cut(s) 113, 558
HapII CCGG 2 cut(s) 114, 281
HgaI GACGC 1 cut(s) 137
HhaI GCGC 1 cut(s) 943
Hin1II CATG 4 cut(s) 392, 568, 884, 1064
Hin6I GCGC 1 cut(s) 941
HinP1I GCGC 1 cut(s) 941
HincII GTYRAC 1 cut(s) 448
HindII GTYRAC 1 cut(s) 448
HindIII AAGCTT 2 cut(s) 395, 1093
HinfI GANTC 2 cut(s) 220, 615
HpaII CCGG 2 cut(s) 114, 281
HphI GGTGA 5 cut(s) 116, 250, 325, 819, 1085
Hpy166II GTNNAC 6 cut(s) 415, 448, 607, 859, 949, 1002
Hpy188III TCNNGA 6 cut(s) 68, 217, 723, 731, 932, 1061
Hpy8I GTNNAC 6 cut(s) 415, 448, 607, 859, 949, 1002
Hpy99I CGWCG 1 cut(s) 277
HpyAV CCTTC 5 cut(s) 34, 320, 428, 461, 1073
HpyCH4III ACNGT 2 cut(s) 273, 412
HpyCH4IV ACGT 4 cut(s) 306, 354, 855, 957
HpyF10VI GCNNNNNNNGC 8 cut(s) 137, 497, 578, 581, 590, 690, 865, 1101
HpyF3I CTNAG 3 cut(s) 29, 141, 198
HpySE526I ACGT 4 cut(s) 306, 354, 855, 957
Hsp92II CATG 4 cut(s) 392, 568, 884, 1064
HspAI GCGC 1 cut(s) 941
Kzo9I GATC 6 cut(s) 76, 213, 484, 704, 934, 1057
LmnI GCTCC 2 cut(s) 116, 374
Lsp1109I GCAGC 5 cut(s) 269, 464, 484, 559, 593
MaeI CTAG 4 cut(s) 120, 474, 731, 944
MaeII ACGT 4 cut(s) 306, 354, 855, 957
MaeIII GTNAC 4 cut(s) 302, 313, 544, 661
MalI GATC 6 cut(s) 78, 215, 486, 706, 936, 1059
MbiI CCGCTC 1 cut(s) 33
MboI GATC 6 cut(s) 76, 213, 484, 704, 934, 1057
MboII GAAGA 3 cut(s) 731, 850, 1021
MflI RGATCY 1 cut(s) 704
MhlI GDGCHC 3 cut(s) 417, 786, 861
MlsI TGGCCA 1 cut(s) 558
MluCI AATT 4 cut(s) 717, 871, 1088, 1107
MluNI TGGCCA 1 cut(s) 558
Mox20I TGGCCA 1 cut(s) 558
MroXI GAANNNNTTC 1 cut(s) 738
MscI TGGCCA 1 cut(s) 558
MseI TTAA 4 cut(s) 915, 927, 1091, 1111
MslI CAYNNNNRTG 3 cut(s) 102, 789, 1065
Msp20I TGGCCA 1 cut(s) 558
MspI CCGG 2 cut(s) 114, 281
MspR9I CCNGG 2 cut(s) 281, 282
Mva1269I GAATGC 2 cut(s) 575, 847
MvnI CGCG 1 cut(s) 941
MwoI GCNNNNNNNGC 8 cut(s) 137, 497, 578, 581, 590, 690, 865, 1101
NciI CCSGG 2 cut(s) 281, 282
NdeII GATC 6 cut(s) 76, 213, 484, 704, 934, 1057
NlaIII CATG 4 cut(s) 392, 568, 884, 1064
NlaIV GGNNCC 1 cut(s) 706
NmuCI GTSAC 2 cut(s) 313, 661
OliI CACNNNNGTG 1 cut(s) 102
PagI TCATGA 1 cut(s) 1060
PctI GAATGC 2 cut(s) 575, 847
PdmI GAANNNNTTC 1 cut(s) 738
PfeI GAWTC 2 cut(s) 220, 615
PflFI GACNNNGTC 1 cut(s) 131
PflMI CCANNNNNTGG 2 cut(s) 18, 565
PkrI GCNGC 8 cut(s) 35, 86, 259, 479, 499, 574, 583, 695
Ppu21I YACGTR 1 cut(s) 958
PspFI CCCAGC 1 cut(s) 382
PspN4I GGNNCC 1 cut(s) 706
PspPI GGNCC 2 cut(s) 111, 1024
PstI CTGCAG 2 cut(s) 482, 502
PsuI RGATCY 1 cut(s) 704
PsyI GACNNNGTC 1 cut(s) 131
RsaI GTAC 1 cut(s) 62
RsaNI GTAC 1 cut(s) 61
RseI CAYNNNNRTG 3 cut(s) 102, 789, 1065
SaqAI TTAA 4 cut(s) 915, 927, 1091, 1111
SatI GCNGC 8 cut(s) 34, 85, 258, 478, 498, 573, 582, 694
Sau3AI GATC 6 cut(s) 76, 213, 484, 704, 934, 1057
Sau96I GGNCC 2 cut(s) 111, 1024
ScrFI CCNGG 2 cut(s) 281, 282
SduI GDGCHC 3 cut(s) 417, 786, 861
SfcI CTRYAG 4 cut(s) 408, 478, 498, 633
SinI GGWCC 1 cut(s) 1024
SmaI CCCGGG 1 cut(s) 282
SmiMI CAYNNNNRTG 3 cut(s) 102, 789, 1065
SmlI CTYRAG 2 cut(s) 492, 968
SmoI CTYRAG 2 cut(s) 492, 968
Sse9I AATT 4 cut(s) 717, 871, 1088, 1107
SsiI CCGC 5 cut(s) 33, 82, 85, 311, 694
SspMI CTAG 4 cut(s) 120, 474, 731, 944
StyD4I CCNGG 2 cut(s) 279, 280
StyI CCWWGG 1 cut(s) 422
TaaI ACNGT 2 cut(s) 273, 412
TaiI ACGT 4 cut(s) 309, 357, 858, 960
TaqI TCGA 2 cut(s) 813, 921
TasI AATT 4 cut(s) 717, 871, 1088, 1107
TauI GCSGC 3 cut(s) 36, 87, 696
TfiI GAWTC 2 cut(s) 220, 615
Tru1I TTAA 4 cut(s) 915, 927, 1091, 1111
Tru9I TTAA 4 cut(s) 915, 927, 1091, 1111
TscAI CASTG 4 cut(s) 384, 417, 559, 651
TseFI GTSAC 2 cut(s) 313, 661
TseI GCWGC 5 cut(s) 257, 477, 497, 572, 581
Tsp45I GTSAC 2 cut(s) 313, 661
TspDTI ATGAA 3 cut(s) 339, 990, 1092
TspMI CCCGGG 1 cut(s) 280
TspRI CASTG 4 cut(s) 384, 417, 559, 651
Tth111I GACNNNGTC 1 cut(s) 131
Van91I CCANNNNNTGG 2 cut(s) 18, 565
VneI GTGCAC 2 cut(s) 413, 857
VpaK11BI GGWCC 1 cut(s) 1024
XapI RAATTY 1 cut(s) 1107
XbaI TCTAGA 1 cut(s) 730
XcmI CCANNNNNNNNNTGG 1 cut(s) 1034
XmaI CCCGGG 1 cut(s) 280
XmnI GAANNNNTTC 1 cut(s) 738
XspI CTAG 4 cut(s) 120, 474, 731, 944
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.