FvH4_1g25760

MACPF domain-containing protein At4g24290-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb1
OG0015525
Physical Location & Seq
Forward (+)
17678834 .. 17685104
6271 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_1g25760.t1

Sequence Viewer

Length: 1800 bp
ATGAGTACCCGGAAAGCTGCATCCGAGGCGGAGGCCGCGATAAAATCCATCGGATTGGGCTACGATCTCACCGTTGACTTGAAGCTCAAGTACTGCAAGCGCCAGTCTTCCGGTGTTGACTCCCGCTTGATCACCATCGACGACGATCAACTCCGTGATATCTCCATTCCCGGCGGTATTTGCATTCCCAATGTCCCCAAGTCCATCAAATGCGATAAGGGCGAGCGCATGCGTTTCGGCTCCGACGTCCTCTCTTTTCAACAGATGTCGGAGCAGTTTAACCAGGAATTGGCCCTTTCGGGTAAGATTCCAACCGGACATTTCAACACTGCGTTTGAGTTTACCGGGGGATGGCAGAAGGATGCCGCCAACACAAAGACTCTTGCTTTCGACGGTGTTTCCATCACCCTTTACAGCGTTGCCCTCGAAAAATCTCAGGTTGCATTGCGTGATGATGTCAAACAAGCTGTTCCTTCATCCTGGGATCCTGCAGCATTGGCAAGGTTTATTGACAAGTATGGAACGCATGTTATAGTTGGGGTGAAAATGGGGGGAAAAGATACGATCTATGCCAAGCAGATGCACTCATCACCTGTCCAACCTGCTGATGTGCAGAAAAAATTGAAGGACATGGCCGACAAGCTGTATATAGATGGAACTGGAACGGGTCACGATAGAGACAAGTTCGCCAAGGATCATGGGCAGGGTATTATGGACGCGATTCCATCCAGTTCTTACGCATCTGCTGAGTCACAGGATATTAAATTTGTGTGCAAGAGAAAGGGTGGAAGCTTACAAAAGAATCTACGCCACAGTGAGTGGTCTCAAACAGTTCAGCTTGAACCTGATGTGATCTCAATGTCATTGGTTCCTATCACATCGTTACTTAGTGGAATTCATGGGAGTGGATTTTTGAGCCATGCCATTAACCTCTATCTTCGTTACAAACCGCCAATTGAAGAACTGCATCAATTTTTAGATTTTCAACTTCCAAGGCAATGGGCACCAGTGTTTGGTGAGCTTGCTCTTGGTCCTCAGAAGAAGCCACAAAGTAGTGCATCTTTACAGTTTAGCTTCATGGGCCCTAAGCTTTACGTTAACACAAATCTGGTTGATGTGGGAAAGAAACCAGTGACGGGTCTCCGACTGTATTTGGAGGGAAAAAGGAGCAATTGCTTAGCAATCCACCTGCAGCATCTTTCATCGCTTCCAAAGACTTTCCAACTCCAGGATCAAGTGAATGAAAATATCTCTCGCCCCTCATCTGACCGCAGATATTTTGAAAAGGTTCAGTGGAAGAGCTTCTCTCATGTCTGCACAGCTCCTATTGAGGCTGATGATTTCAATGTTGTCACTGGGGCTCACTTCGAAATTGGAGAGTCGGGGATGAAAAAAGTATTGTTTTTACGGCTTCATTTCTCTAAAGTTATGGGTGCAACCAATGTTAGACAATCTGAATGGGACGGTTCACCAGCCTTGACACAGAAATCAGGTATTATTTCAACCATGATCAGCACTCGGTTCTCGTCTGCTGCTCAAAAACAGCCCCCTAATCCTGCCGATGTGAACATAAATTCGGCTGTGTATCCTGGAGGACCCCCGGTACCCACACAGACACCTAAACTACTGAGGTTCGTCGACACAACAGAAATGACTAGAGGACCACAGGATTCACCTGGGTATTGGGTTGTGTCGGGTGCAAGGCTGCTCGTTGAGAAGGCTAAAATTTCTTTACGAGTTAAGTATTCTCTCCTGACCATGATTTTACCTGACGAAGAAGAGATGCAAGATGGCTACTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
MACPF

Protein Analysis

600

Amino Acids

66.18

Weight (kDa)

8.88

Isoelectric Point (pI)

41.78

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
MACPF PF01823 107 - 312 1.3e-37 MAC/Perforin domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0015525)

Species Orthologous Gene IDs
fragaria_vesca FvH4_1g25760 FvH4_1g25760
malus_domestica MD15G1140100.v1.1
prunus_persica Prupe.4G285900_v2.0.a1
pyrus_communis pycom15g12660
rosa_chinensis RchiOBHm_Chr3g0484761
rosa_laevigata RLG00000023205
rosa_multiflora Rmu_sc0001689.1_g000010 Rmu_sc0006653.1_g000005
rosa_roxburghii Rroxscaffold_6G00397460
rosa_rugosa Rorug03G0213800
rosa_samantha Rh3AG264100 Rh3BG299100 Rh3CG296900 Rh3DG292700
rosa_wichuraiana Rw3G023550

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 1197
AatII GACGTC 1 cut(s) 249
Acc36I ACCTGC 2 cut(s) 610, 1197
Acc65I GGTACC 1 cut(s) 1603
AccB1I GGYRCC 2 cut(s) 1003, 1603
AccB7I CCANNNNNTGG 2 cut(s) 289, 1013
AccI GTMKAC 1 cut(s) 1638
AccII CGCG 2 cut(s) 38, 719
AciI CCGC 7 cut(s) 29, 36, 124, 174, 366, 950, 1270
AclWI GGATC 4 cut(s) 479, 492, 702, 1239
AcoI YGGCCR 1 cut(s) 633
AcsI RAATTY 4 cut(s) 764, 894, 1573, 1725
AcyI GRCGYC 1 cut(s) 246
AfaI GTAC 3 cut(s) 7, 92, 1605
AfiI CCNNNNNNNGG 5 cut(s) 289, 351, 1013, 1136, 1228
AjnI CCWGG 5 cut(s) 282, 479, 1227, 1588, 1675
Alw26I GTCTC 3 cut(s) 672, 828, 1145
AlwI GGATC 4 cut(s) 479, 492, 702, 1239
AoxI GGCC 4 cut(s) 33, 291, 633, 1081
ApaI GGGCCC 1 cut(s) 1085
ApeKI GCWGC 5 cut(s) 17, 491, 1192, 1532, 1705
ApoI RAATTY 4 cut(s) 764, 894, 1573, 1725
Asp700I GAANNNNTTC 2 cut(s) 1287, 1301
Asp718I GGTACC 1 cut(s) 1603
AspLEI GCGC 2 cut(s) 102, 228
AspS9I GGNCC 6 cut(s) 292, 1031, 1081, 1082, 1595, 1661
AsuC2I CCSGG 4 cut(s) 10, 171, 346, 1601
AsuHPI GGTGA 8 cut(s) 61, 124, 397, 553, 582, 1028, 1461, 1665
AsuII TTCGAA 1 cut(s) 1368
AvaII GGWCC 3 cut(s) 1031, 1595, 1661
BaeGI GKGCMC 2 cut(s) 1006, 1085
BamHI GGATCC 1 cut(s) 484
BanI GGYRCC 2 cut(s) 1003, 1603
BanII GRGCYC 2 cut(s) 1085, 1363
BarI GAAGNNNNNNTAC 2 cut(s) 74, 106
BbsI GAAGAC 1 cut(s) 99
BbvI GCAGC 5 cut(s) 4, 503, 1204, 1519, 1692
BccI CCATC 8 cut(s) 56, 143, 212, 345, 410, 647, 733, 1784
BceAI ACGGC 1 cut(s) 1424
BcgI CGANNNNNNTGC 2 cut(s) 217, 251
BciT130I CCWGG 5 cut(s) 284, 481, 1229, 1590, 1677
BciVI GTATCC 1 cut(s) 1596
BclI TGATCA 2 cut(s) 129, 1509
BcnI CCSGG 4 cut(s) 10, 171, 346, 1601
BcoDI GTCTC 3 cut(s) 672, 828, 1145
BfaI CTAG 1 cut(s) 1656
BfmI CTRYAG 2 cut(s) 489, 1190
BfoI RGCGCY 1 cut(s) 103
BfuAI ACCTGC 2 cut(s) 610, 1197
BfuI GTATCC 1 cut(s) 1596
BisI GCNGC 7 cut(s) 18, 36, 366, 492, 1193, 1533, 1706
BlpI GCTNAGC 1 cut(s) 1177
BlsI GCNGC 7 cut(s) 19, 37, 367, 493, 1194, 1534, 1707
BmcAI AGTACT 1 cut(s) 92
Bme1390I CCNGG 9 cut(s) 10, 171, 284, 346, 481, 1229, 1590, 1601, 1677
Bme18I GGWCC 3 cut(s) 1031, 1595, 1661
BmgT120I GGNCC 6 cut(s) 292, 1031, 1081, 1082, 1595, 1661
BmiI GGNNCC 7 cut(s) 241, 486, 870, 1005, 1083, 1597, 1605
BmrFI CCNGG 9 cut(s) 10, 171, 284, 346, 481, 1229, 1590, 1601, 1677
BmrI ACTGGG 1 cut(s) 1365
BmsI GCATC 8 cut(s) 29, 352, 570, 749, 976, 1067, 1204, 1773
BmuI ACTGGG 1 cut(s) 1365
BpiI GAAGAC 1 cut(s) 99
BplI GAGNNNNNCTC 2 cut(s) 1291, 1323
BpmI CTGGAG 2 cut(s) 1211, 1611
Bpu10I CCTNAGC 1 cut(s) 1086
Bpu1102I GCTNAGC 1 cut(s) 1177
Bpu14I TTCGAA 1 cut(s) 1368
BpuEI CTTGAG 1 cut(s) 71
BpuMI CCSGG 4 cut(s) 10, 171, 346, 1601
BsaBI GATNNNNATC 1 cut(s) 134
BsaHI GRCGYC 1 cut(s) 246
BsaI GGTCTC 2 cut(s) 828, 1145
BsaJI CCNNGG 7 cut(s) 24, 345, 480, 690, 992, 1599, 1676
BsaWI WCCGGW 2 cut(s) 110, 314
Bsc4I CCNNNNNNNGG 5 cut(s) 289, 351, 1013, 1136, 1228
Bse1I ACTGG 6 cut(s) 103, 664, 729, 1007, 1130, 1360
Bse3DI GCAATG 2 cut(s) 443, 1004
Bse8I GATNNNNATC 1 cut(s) 134
BseBI CCWGG 5 cut(s) 284, 481, 1229, 1590, 1677
BseDI CCNNGG 7 cut(s) 24, 345, 480, 690, 992, 1599, 1676
BseGI GGATG 6 cut(s) 20, 356, 367, 476, 725, 1392
BseJI GATNNNNATC 1 cut(s) 134
BseLI CCNNNNNNNGG 5 cut(s) 289, 351, 1013, 1136, 1228
BseMI GCAATG 2 cut(s) 443, 1004
BseMII CTCAG 4 cut(s) 449, 738, 1049, 1619
BseNI ACTGG 6 cut(s) 103, 664, 729, 1007, 1130, 1360
BseSI GKGCMC 2 cut(s) 1006, 1085
BseXI GCAGC 5 cut(s) 4, 503, 1204, 1519, 1692
BsgI GTGCAG 2 cut(s) 632, 1300
Bsh1236I CGCG 2 cut(s) 38, 719
BshFI GGCC 4 cut(s) 35, 293, 635, 1083
BshNI GGYRCC 2 cut(s) 1003, 1603
BsiSI CCGG 6 cut(s) 10, 111, 171, 315, 345, 1601
BslFI GGGAC 2 cut(s) 179, 1475
BslI CCNNNNNNNGG 5 cut(s) 289, 351, 1013, 1136, 1228
BsmAI GTCTC 3 cut(s) 672, 828, 1145
BsmFI GGGAC 2 cut(s) 179, 1475
BsmI GAATGC 1 cut(s) 183
BsnI GGCC 4 cut(s) 35, 293, 635, 1083
Bso31I GGTCTC 2 cut(s) 828, 1145
Bsp119I TTCGAA 1 cut(s) 1368
Bsp120I GGGCCC 1 cut(s) 1081
Bsp1286I GDGCHC 3 cut(s) 1006, 1085, 1363
Bsp143I GATC 9 cut(s) 64, 129, 145, 484, 564, 694, 852, 1231, 1509
Bsp1720I GCTNAGC 1 cut(s) 1177
BspACI CCGC 7 cut(s) 29, 36, 124, 174, 366, 950, 1270
BspANI GGCC 4 cut(s) 35, 293, 635, 1083
BspCNI CTCAG 4 cut(s) 448, 739, 1048, 1620
BspFNI CGCG 2 cut(s) 38, 719
BspLI GGNNCC 7 cut(s) 241, 486, 870, 1005, 1083, 1597, 1605
BspMAI CTGCAG 2 cut(s) 493, 1194
BspMI ACCTGC 2 cut(s) 610, 1197
BspPI GGATC 4 cut(s) 479, 492, 702, 1239
BspQI GCTCTTC 1 cut(s) 1292
BspT104I TTCGAA 1 cut(s) 1368
BspT107I GGYRCC 2 cut(s) 1003, 1603
BspTNI GGTCTC 2 cut(s) 828, 1145
BsrDI GCAATG 2 cut(s) 443, 1004
BsrI ACTGG 6 cut(s) 103, 664, 729, 1007, 1130, 1360
BssECI CCNNGG 7 cut(s) 24, 345, 480, 690, 992, 1599, 1676
BssMI GATC 9 cut(s) 64, 129, 145, 484, 564, 694, 852, 1231, 1509
BssNI GRCGYC 1 cut(s) 246
BssT1I CCWWGG 2 cut(s) 690, 992
Bst2UI CCWGG 5 cut(s) 284, 481, 1229, 1590, 1677
Bst4CI ACNGT 7 cut(s) 73, 395, 815, 832, 1068, 1149, 1466
Bst6I CTCTTC 2 cut(s) 1292, 1773
BstACI GRCGYC 1 cut(s) 246
BstBI TTCGAA 1 cut(s) 1368
BstC8I GCNNGC 4 cut(s) 98, 224, 230, 1023
BstDEI CTNAG 7 cut(s) 435, 747, 887, 1035, 1086, 1177, 1628
BstF5I GGATG 6 cut(s) 20, 356, 367, 476, 725, 1392
BstFNI CGCG 2 cut(s) 38, 719
BstH2I RGCGCY 1 cut(s) 103
BstHHI GCGC 2 cut(s) 102, 228
BstKTI GATC 9 cut(s) 67, 132, 148, 487, 567, 697, 855, 1234, 1512
BstMAI GTCTC 3 cut(s) 672, 828, 1145
BstMBI GATC 9 cut(s) 64, 129, 145, 484, 564, 694, 852, 1231, 1509
BstMWI GCNNNNNNNGC 6 cut(s) 26, 35, 180, 219, 497, 1080
BstNI CCWGG 5 cut(s) 284, 481, 1229, 1590, 1677
BstNSI RCATGY 2 cut(s) 232, 530
BstSCI CCNGG 9 cut(s) 8, 169, 282, 344, 479, 1227, 1588, 1599, 1675
BstSFI CTRYAG 2 cut(s) 489, 1190
BstSLI GKGCMC 2 cut(s) 1006, 1085
BstUI CGCG 2 cut(s) 38, 719
BstV1I GCAGC 5 cut(s) 4, 503, 1204, 1519, 1692
BstV2I GAAGAC 1 cut(s) 99
BstX2I RGATCY 1 cut(s) 484
BstXI CCANNNNNNTGG 2 cut(s) 55, 999
BstYI RGATCY 1 cut(s) 484
BsuI GTATCC 1 cut(s) 1596
BsuRI GGCC 4 cut(s) 35, 293, 635, 1083
BtgZI GCGATG 1 cut(s) 1188
BtsCI GGATG 6 cut(s) 20, 356, 367, 476, 725, 1392
BtsI GCAGTG 1 cut(s) 327
BtsIMutI CAGTG 6 cut(s) 327, 820, 1014, 1137, 1298, 1353
BveI ACCTGC 2 cut(s) 610, 1197
Cac8I GCNNGC 4 cut(s) 98, 224, 230, 1023
CfoI GCGC 2 cut(s) 102, 228
Cfr13I GGNCC 6 cut(s) 292, 1031, 1081, 1082, 1595, 1661
CseI GACGC 1 cut(s) 725
Csp6I GTAC 3 cut(s) 6, 91, 1604
CviQI GTAC 3 cut(s) 6, 91, 1604
DdeI CTNAG 7 cut(s) 435, 747, 887, 1035, 1086, 1177, 1628
DpnI GATC 9 cut(s) 66, 131, 147, 486, 566, 696, 854, 1233, 1511
DpnII GATC 9 cut(s) 64, 129, 145, 484, 564, 694, 852, 1231, 1509
EaeI YGGCCR 1 cut(s) 633
Eam1104I CTCTTC 2 cut(s) 1292, 1773
EarI CTCTTC 2 cut(s) 1292, 1773
EciI GGCGGA 1 cut(s) 44
Eco130I CCWWGG 2 cut(s) 690, 992
Eco24I GRGCYC 2 cut(s) 1085, 1363
Eco31I GGTCTC 2 cut(s) 828, 1145
Eco32I GATATC 1 cut(s) 160
Eco47I GGWCC 3 cut(s) 1031, 1595, 1661
EcoO109I RGGNCCY 2 cut(s) 1082, 1595
EcoRI GAATTC 1 cut(s) 894
EcoRII CCWGG 5 cut(s) 282, 479, 1227, 1588, 1675
EcoRV GATATC 1 cut(s) 160
EcoT14I CCWWGG 2 cut(s) 690, 992
EcoT38I GRGCYC 2 cut(s) 1085, 1363
ErhI CCWWGG 2 cut(s) 690, 992
FaqI GGGAC 2 cut(s) 179, 1475
FauI CCCGC 1 cut(s) 131
FbaI TGATCA 2 cut(s) 129, 1509
FblI GTMKAC 1 cut(s) 1638
Fnu4HI GCNGC 7 cut(s) 18, 36, 366, 492, 1193, 1533, 1706
FokI GGATG 6 cut(s) 7, 363, 374, 463, 712, 1399
FriOI GRGCYC 2 cut(s) 1085, 1363
Fsp4HI GCNGC 7 cut(s) 18, 36, 366, 492, 1193, 1533, 1706
FspBI CTAG 1 cut(s) 1656
GlaI GCGC 2 cut(s) 101, 227
GluI GCNGC 7 cut(s) 18, 36, 366, 492, 1193, 1533, 1706
GsuI CTGGAG 2 cut(s) 1211, 1611
HaeII RGCGCY 1 cut(s) 103
HaeIII GGCC 4 cut(s) 35, 293, 635, 1083
HapII CCGG 6 cut(s) 10, 111, 171, 315, 345, 1601
HgaI GACGC 1 cut(s) 725
HhaI GCGC 2 cut(s) 102, 228
Hin1I GRCGYC 1 cut(s) 246
Hin6I GCGC 2 cut(s) 100, 226
HinP1I GCGC 2 cut(s) 100, 226
HincII GTYRAC 4 cut(s) 76, 118, 1099, 1639
HindII GTYRAC 4 cut(s) 76, 118, 1099, 1639
HindIII AAGCTT 2 cut(s) 790, 1088
HinfI GANTC 8 cut(s) 119, 307, 379, 721, 749, 802, 1379, 1670
HpaI GTTAAC 1 cut(s) 1099
HpaII CCGG 6 cut(s) 10, 111, 171, 315, 345, 1601
HphI GGTGA 8 cut(s) 61, 124, 397, 553, 582, 1028, 1461, 1665
Hpy166II GTNNAC 7 cut(s) 76, 118, 342, 1099, 1469, 1567, 1639
Hpy188I TCNGA 8 cut(s) 25, 53, 244, 271, 1038, 1145, 1267, 1456
Hpy188III TCNNGA 2 cut(s) 671, 1753
Hpy8I GTNNAC 7 cut(s) 76, 118, 342, 1099, 1469, 1567, 1639
Hpy99I CGWCG 5 cut(s) 143, 146, 248, 395, 1640
HpyAV CCTTC 4 cut(s) 352, 483, 619, 1711
HpyCH4III ACNGT 7 cut(s) 73, 395, 815, 832, 1068, 1149, 1466
HpyCH4IV ACGT 2 cut(s) 246, 1095
HpyF10VI GCNNNNNNNGC 6 cut(s) 26, 35, 180, 219, 497, 1080
HpyF3I CTNAG 7 cut(s) 435, 747, 887, 1035, 1086, 1177, 1628
HpySE526I ACGT 2 cut(s) 246, 1095
Hsp92I GRCGYC 1 cut(s) 246
HspAI GCGC 2 cut(s) 100, 226
KpnI GGTACC 1 cut(s) 1607
Ksp22I TGATCA 2 cut(s) 129, 1509
KspAI GTTAAC 1 cut(s) 1099
Kzo9I GATC 9 cut(s) 64, 129, 145, 484, 564, 694, 852, 1231, 1509
LguI GCTCTTC 1 cut(s) 1292
LmnI GCTCC 4 cut(s) 245, 271, 1167, 1327
Lsp1109I GCAGC 5 cut(s) 4, 503, 1204, 1519, 1692
LweI GCATC 8 cut(s) 29, 352, 570, 749, 976, 1067, 1204, 1773
MaeI CTAG 1 cut(s) 1656
MaeII ACGT 2 cut(s) 246, 1095
MaeIII GTNAC 6 cut(s) 668, 750, 882, 941, 1132, 1351
MalI GATC 9 cut(s) 66, 131, 147, 486, 566, 696, 854, 1233, 1511
MboI GATC 9 cut(s) 64, 129, 145, 484, 564, 694, 852, 1231, 1509
MboII GAAGA 7 cut(s) 99, 929, 971, 1051, 1309, 1787, 1790
MfeI CAATTG 2 cut(s) 954, 1171
MflI RGATCY 1 cut(s) 484
MhlI GDGCHC 3 cut(s) 1006, 1085, 1363
MlyI GAGTC 4 cut(s) 113, 373, 758, 1388
MmeI TCCRAC 6 cut(s) 249, 267, 335, 622, 1168, 1246
MroXI GAANNNNTTC 2 cut(s) 1287, 1301
MseI TTAA 5 cut(s) 279, 762, 927, 1098, 1740
MslI CAYNNNNRTG 1 cut(s) 903
MspI CCGG 6 cut(s) 10, 111, 171, 315, 345, 1601
MspR9I CCNGG 9 cut(s) 10, 171, 284, 346, 481, 1229, 1590, 1601, 1677
MunI CAATTG 2 cut(s) 954, 1171
Mva1269I GAATGC 1 cut(s) 183
MvaI CCWGG 5 cut(s) 284, 481, 1229, 1590, 1677
MvnI CGCG 2 cut(s) 38, 719
MwoI GCNNNNNNNGC 6 cut(s) 26, 35, 180, 219, 497, 1080
NciI CCSGG 4 cut(s) 10, 171, 346, 1601
NdeII GATC 9 cut(s) 64, 129, 145, 484, 564, 694, 852, 1231, 1509
NlaIV GGNNCC 7 cut(s) 241, 486, 870, 1005, 1083, 1597, 1605
NmuCI GTSAC 4 cut(s) 668, 750, 1132, 1351
NspI RCATGY 2 cut(s) 232, 530
NspV TTCGAA 1 cut(s) 1368
PaeI GCATGC 1 cut(s) 232
PaqCI CACCTGC 1 cut(s) 1197
PciSI GCTCTTC 1 cut(s) 1292
PcsI WCGNNNNNNNCGW 2 cut(s) 69, 243
PctI GAATGC 1 cut(s) 183
PdmI GAANNNNTTC 2 cut(s) 1287, 1301
PfeI GAWTC 4 cut(s) 307, 721, 802, 1670
PflMI CCANNNNNTGG 2 cut(s) 289, 1013
PfoI TCCNGGA 2 cut(s) 1227, 1588
PkrI GCNGC 7 cut(s) 19, 37, 367, 493, 1194, 1534, 1707
PleI GAGTC 4 cut(s) 113, 373, 757, 1387
PpsI GAGTC 4 cut(s) 113, 373, 757, 1387
PpuMI RGGWCCY 1 cut(s) 1595
Psp5II RGGWCCY 1 cut(s) 1595
Psp6I CCWGG 5 cut(s) 282, 479, 1227, 1588, 1675
PspGI CCWGG 5 cut(s) 282, 479, 1227, 1588, 1675
PspN4I GGNNCC 7 cut(s) 241, 486, 870, 1005, 1083, 1597, 1605
PspOMI GGGCCC 1 cut(s) 1081
PspPI GGNCC 6 cut(s) 292, 1031, 1081, 1082, 1595, 1661
PspPPI RGGWCCY 1 cut(s) 1595
PstI CTGCAG 2 cut(s) 493, 1194
PsuI RGATCY 1 cut(s) 484
RsaI GTAC 3 cut(s) 7, 92, 1605
RsaNI GTAC 3 cut(s) 6, 91, 1604
RseI CAYNNNNRTG 1 cut(s) 903
SalI GTCGAC 1 cut(s) 1637
SapI GCTCTTC 1 cut(s) 1292
SaqAI TTAA 5 cut(s) 279, 762, 927, 1098, 1740
SatI GCNGC 7 cut(s) 18, 36, 366, 492, 1193, 1533, 1706
Sau3AI GATC 9 cut(s) 64, 129, 145, 484, 564, 694, 852, 1231, 1509
Sau96I GGNCC 6 cut(s) 292, 1031, 1081, 1082, 1595, 1661
ScaI AGTACT 1 cut(s) 92
SchI GAGTC 4 cut(s) 113, 373, 758, 1388
ScrFI CCNGG 9 cut(s) 10, 171, 284, 346, 481, 1229, 1590, 1601, 1677
SduI GDGCHC 3 cut(s) 1006, 1085, 1363
SfaNI GCATC 8 cut(s) 29, 352, 570, 749, 976, 1067, 1204, 1773
SfcI CTRYAG 2 cut(s) 489, 1190
SfuI TTCGAA 1 cut(s) 1368
SinI GGWCC 3 cut(s) 1031, 1595, 1661
SmiMI CAYNNNNRTG 1 cut(s) 903
SmlI CTYRAG 1 cut(s) 86
SmoI CTYRAG 1 cut(s) 86
SphI GCATGC 1 cut(s) 232
SsiI CCGC 7 cut(s) 29, 36, 124, 174, 366, 950, 1270
SspMI CTAG 1 cut(s) 1656
StyD4I CCNGG 9 cut(s) 8, 169, 282, 344, 479, 1227, 1588, 1599, 1675
StyI CCWWGG 2 cut(s) 690, 992
TaaI ACNGT 7 cut(s) 73, 395, 815, 832, 1068, 1149, 1466
TaiI ACGT 2 cut(s) 249, 1098
TaqI TCGA 5 cut(s) 138, 390, 426, 1368, 1638
TatI WGTACW 1 cut(s) 90
TauI GCSGC 2 cut(s) 38, 368
TfiI GAWTC 4 cut(s) 307, 721, 802, 1670
Tru1I TTAA 5 cut(s) 279, 762, 927, 1098, 1740
Tru9I TTAA 5 cut(s) 279, 762, 927, 1098, 1740
TscAI CASTG 6 cut(s) 334, 820, 1014, 1137, 1298, 1360
TseFI GTSAC 4 cut(s) 668, 750, 1132, 1351
TseI GCWGC 5 cut(s) 17, 491, 1192, 1532, 1705
Tsp45I GTSAC 4 cut(s) 668, 750, 1132, 1351
TspDTI ATGAA 7 cut(s) 465, 887, 1066, 1191, 1257, 1403, 1403
TspGWI ACGGA 1 cut(s) 143
TspRI CASTG 6 cut(s) 334, 820, 1014, 1137, 1298, 1360
Van91I CCANNNNNTGG 2 cut(s) 289, 1013
VpaK11BI GGWCC 3 cut(s) 1031, 1595, 1661
XapI RAATTY 4 cut(s) 764, 894, 1573, 1725
XceI RCATGY 2 cut(s) 232, 530
XmiI GTMKAC 1 cut(s) 1638
XmnI GAANNNNTTC 2 cut(s) 1287, 1301
XspI CTAG 1 cut(s) 1656
ZraI GACGTC 1 cut(s) 247
ZrmI AGTACT 1 cut(s) 92
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.