Rh3DG292700

MACPF domain-containing protein At4g24290-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr3D
OG0015525
Physical Location & Seq
Forward (+)
30355938 .. 30361964
6027 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh3DG292700.1

Sequence Viewer

Length: 1797 bp
ATGAGTACTCGGAAAGCTGCGGTTGAGGCGGACGCTGCGATAAAATCGATCGGGCTGGGCTACGATCTCACCGTTGACTTGAAGCTCAAGTACTGCAAGCGCCAGTCTTCCGGTGTTGACTCGCGCTTGATCGCCGTAGACGACGATCAAGTCCGAGATATCGCTATTCCCGGCGGGATTTGCATACCCAGTGTCCCCAAGTCCATCAAATGCGATAAGGGCGAGCGCATGAGGTTCGGCTCCGAGGTCCTTTCATTTCAACAGATGTCAGAGCAGTTTAACCAGGAACTGGCTCTGTCCGGAAAAATTCCGACCGGACATTTCAACACTGCGTTTGAGTTTACTGGAGGATGGCAGAAGGATGCTGCCAACACAAAGACTCTTGCTTTCGACGGGGTTTCGATCACCCTCTACAGCGTTGCTCTTGAAAAATCTCAAGTAGCCTTGCGTGATGATGTCAAACAAGCTGTTCCTTCTTCCTGGGATCCTGCAGCATTGGCAAGGTTTATTGAGAAGTACGGAACTCATGTTATAGTTGGGGTGAAGATGGGGGGAAAGGATACGATATACGCCAAGCAGATGCACTCTTCACCTGTCCAACCTGCTGATGTGCAGAAAAAATTGAAGGACATGGCAGACAAAATGTATATAGATGGAACTGGAACTGGTCACGAAAGAGACAAGTTCGCCAAGGAACATGGGCAGGGTATCATGAACGCGATTCCATCCAGTTCTTACGCATCTGTTGAGTCACAGGATATTAAATTTGTGTGCAAGAGAAAGGGTGGAAACTTACAAAAAAATCTACGCCACAGTGAGTGGTCTCAAACAGTTCAGCTTGAACCTGATGTGATCTCAATGTCATTGGTTCCTATCACATCGTTACTGAGTGGAGTTCATGGGAGTGGATTTTTGAGCCATGCCATTAACCTCTATCTTCGTTACAAACCGCCAATTGAAGAACTACATCAATTTTTGGAATTTCAGCTTCCAAGGCAATGGGCACCGGTGTTTGGTGAGCTTGCTCTTGGTCCTCAGAAGAAGCAACAAAGTGGTGCATCTTTACAGTTTAGCTTCATGGGCCCTAAGCTTTACGTTAACACAAATCCGGTTGATGTGGGGAAGAAACCAGTGACGGGTCTCCGACTGTATTTGGAGGGAAAAAAGAGCAATTGCTTAGCAATCCACCTGCAGCATCTTTCAACGCTGCCAAAGACTTTCCAACTCCAGGATCATTTGCATATCTCTCATCCCTCATCTGACCGCAGATATTATGAGAAGGTTCAGTGGAAGAGCTTCTCTCATGTCTGCACAGCTCCTATTGAGGCTAATGATGATTTCTCTGTTGTCACTGGGGCTCACTTCGAAATTGGAGAGTCGGGGATGAAAAAAGTATTATTTTTACGACTTCATTTCTCTAAAGTCAGTGGTGCAACCAATGTTAGACAATCTGAGTGGGACGGTTCCCCAGCCTTGACACAGAAATCGGGTATTATTTCAACCATTATCAGCACTCGTTTCTCGTCTGCTGCCCAAAAACAGCCCCCTAATCCTGCTGATGTGAACATAAATTCGGCTTTGTATCCTGGAGGTCCCCCGGTACCCACACAGACACCTAAACTAATGAGGTTTGTCGACACAACAGAATTGACTAGAGGACCGCAGGATTCACCTGGATATTGGGTTGTGTCCGGTGCAAGGCTGCTTGTTGAGAAGGCTAAAATATCTTTACGAGTCAAGTATTCTCTCCTGACCATGATTTTACCTGACGAAGAAGAGATGCAAGATGGCTATTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
MACPF

Protein Analysis

598

Amino Acids

66.02

Weight (kDa)

8.95

Isoelectric Point (pI)

41.3

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
MACPF PF01823 107 - 312 6.4e-39 MAC/Perforin domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0015525)

Species Orthologous Gene IDs
fragaria_vesca FvH4_1g25760 FvH4_1g25760
malus_domestica MD15G1140100.v1.1
prunus_persica Prupe.4G285900_v2.0.a1
pyrus_communis pycom15g12660
rosa_chinensis RchiOBHm_Chr3g0484761
rosa_laevigata RLG00000023205
rosa_multiflora Rmu_sc0001689.1_g000010 Rmu_sc0006653.1_g000005
rosa_roxburghii Rroxscaffold_6G00397460
rosa_rugosa Rorug03G0213800
rosa_samantha Rh3AG264100 Rh3BG299100 Rh3CG296900 Rh3DG292700
rosa_wichuraiana Rw3G023550

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 1197
AasI GACNNNNNNGTC 1 cut(s) 149
Acc36I ACCTGC 2 cut(s) 610, 1197
Acc65I GGTACC 1 cut(s) 1600
AccB1I GGYRCC 2 cut(s) 1003, 1600
AccB7I CCANNNNNTGG 1 cut(s) 289
AccI GTMKAC 2 cut(s) 138, 1635
AccII CGCG 2 cut(s) 124, 719
AccIII TCCGGA 1 cut(s) 299
AciI CCGC 6 cut(s) 20, 29, 174, 950, 1264, 1661
AclWI GGATC 3 cut(s) 479, 492, 1239
AcsI RAATTY 4 cut(s) 306, 764, 980, 1570
AfaI GTAC 4 cut(s) 7, 92, 518, 1602
AfiI CCNNNNNNNGG 5 cut(s) 289, 1013, 1136, 1228, 1698
AgeI ACCGGT 1 cut(s) 1006
AgsI TTSAA 9 cut(s) 82, 260, 325, 428, 625, 842, 959, 1203, 1500
AjnI CCWGG 5 cut(s) 282, 479, 1227, 1585, 1672
Alw26I GTCTC 3 cut(s) 672, 828, 1145
AlwI GGATC 3 cut(s) 479, 492, 1239
AlwNI CAGNNNCTG 1 cut(s) 289
Aor13HI TCCGGA 1 cut(s) 299
AoxI GGCC 1 cut(s) 1081
ApaI GGGCCC 1 cut(s) 1085
ApeKI GCWGC 8 cut(s) 17, 35, 365, 491, 1192, 1207, 1529, 1702
ApoI RAATTY 4 cut(s) 306, 764, 980, 1570
AsiGI ACCGGT 1 cut(s) 1006
Asp700I GAANNNNTTC 1 cut(s) 1295
Asp718I GGTACC 1 cut(s) 1600
AspLEI GCGC 3 cut(s) 102, 126, 228
AspS9I GGNCC 6 cut(s) 247, 1031, 1081, 1082, 1592, 1658
AsuC2I CCSGG 2 cut(s) 171, 1598
AsuHPI GGTGA 6 cut(s) 61, 397, 553, 582, 1028, 1662
AsuII TTCGAA 1 cut(s) 1365
AvaII GGWCC 4 cut(s) 247, 1031, 1592, 1658
BaeGI GKGCMC 2 cut(s) 1006, 1085
BamHI GGATCC 1 cut(s) 484
BanI GGYRCC 2 cut(s) 1003, 1600
BanII GRGCYC 2 cut(s) 1085, 1360
BarI GAAGNNNNNNTAC 2 cut(s) 74, 106
BbsI GAAGAC 1 cut(s) 99
BbvI GCAGC 8 cut(s) 4, 22, 352, 503, 1194, 1204, 1516, 1689
BccI CCATC 6 cut(s) 212, 345, 541, 647, 733, 1781
BceAI ACGGC 1 cut(s) 119
BcgI CGANNNNNNTGC 2 cut(s) 217, 251
BciT130I CCWGG 5 cut(s) 284, 481, 1229, 1587, 1674
BciVI GTATCC 2 cut(s) 553, 1593
BcnI CCSGG 2 cut(s) 171, 1598
BcoDI GTCTC 3 cut(s) 672, 828, 1145
BfaI CTAG 1 cut(s) 1653
BfmI CTRYAG 3 cut(s) 412, 489, 1190
BfoI RGCGCY 1 cut(s) 103
BfuAI ACCTGC 2 cut(s) 610, 1197
BfuI GTATCC 2 cut(s) 553, 1593
BisI GCNGC 8 cut(s) 18, 36, 366, 492, 1193, 1208, 1530, 1703
BlpI GCTNAGC 1 cut(s) 1177
BlsI GCNGC 8 cut(s) 19, 37, 367, 493, 1194, 1209, 1531, 1704
BmcAI AGTACT 2 cut(s) 7, 92
Bme1390I CCNGG 7 cut(s) 171, 284, 481, 1229, 1587, 1598, 1674
Bme18I GGWCC 4 cut(s) 247, 1031, 1592, 1658
BmgT120I GGNCC 6 cut(s) 247, 1031, 1081, 1082, 1592, 1658
BmiI GGNNCC 8 cut(s) 241, 486, 870, 1005, 1083, 1465, 1594, 1602
BmrFI CCNGG 7 cut(s) 171, 284, 481, 1229, 1587, 1598, 1674
BmrI ACTGGG 2 cut(s) 183, 1362
BmsI GCATC 6 cut(s) 352, 570, 749, 1067, 1204, 1770
BmuI ACTGGG 2 cut(s) 183, 1362
BpiI GAAGAC 1 cut(s) 99
BplI GAGNNNNNCTC 2 cut(s) 1285, 1317
BpmI CTGGAG 3 cut(s) 366, 1211, 1608
Bpu10I CCTNAGC 1 cut(s) 1086
Bpu1102I GCTNAGC 1 cut(s) 1177
Bpu14I TTCGAA 1 cut(s) 1365
BpuEI CTTGAG 2 cut(s) 71, 420
BpuMI CCSGG 2 cut(s) 171, 1598
Bsa29I ATCGAT 1 cut(s) 47
BsaI GGTCTC 2 cut(s) 828, 1145
BsaJI CCNNGG 5 cut(s) 243, 480, 690, 992, 1596
BsaWI WCCGGW 6 cut(s) 110, 299, 314, 1006, 1108, 1691
Bsc4I CCNNNNNNNGG 5 cut(s) 289, 1013, 1136, 1228, 1698
Bse118I RCCGGY 1 cut(s) 1006
Bse1I ACTGG 9 cut(s) 103, 189, 294, 349, 664, 670, 729, 1130, 1357
Bse3DI GCAATG 1 cut(s) 1004
BseAI TCCGGA 1 cut(s) 299
BseBI CCWGG 5 cut(s) 284, 481, 1229, 1587, 1674
BseCI ATCGAT 1 cut(s) 47
BseDI CCNNGG 5 cut(s) 243, 480, 690, 992, 1596
BseGI GGATG 5 cut(s) 356, 367, 725, 1249, 1389
BseLI CCNNNNNNNGG 5 cut(s) 289, 1013, 1136, 1228, 1698
BseMI GCAATG 1 cut(s) 1004
BseMII CTCAG 3 cut(s) 878, 1049, 1443
BseNI ACTGG 9 cut(s) 103, 189, 294, 349, 664, 670, 729, 1130, 1357
BseSI GKGCMC 2 cut(s) 1006, 1085
BseXI GCAGC 8 cut(s) 4, 22, 352, 503, 1194, 1204, 1516, 1689
BseYI CCCAGC 2 cut(s) 55, 1468
BsgI GTGCAG 2 cut(s) 632, 1294
Bsh1236I CGCG 2 cut(s) 124, 719
Bsh1285I CGRYCG 2 cut(s) 51, 315
BshFI GGCC 1 cut(s) 1083
BshNI GGYRCC 2 cut(s) 1003, 1600
BshTI ACCGGT 1 cut(s) 1006
BshVI ATCGAT 1 cut(s) 47
BsiEI CGRYCG 2 cut(s) 51, 315
BsiSI CCGG 8 cut(s) 111, 171, 300, 315, 1007, 1109, 1598, 1692
BslFI GGGAC 3 cut(s) 179, 1472, 1578
BslI CCNNNNNNNGG 5 cut(s) 289, 1013, 1136, 1228, 1698
BsmAI GTCTC 3 cut(s) 672, 828, 1145
BsmFI GGGAC 3 cut(s) 179, 1472, 1578
BsnI GGCC 1 cut(s) 1083
Bso31I GGTCTC 2 cut(s) 828, 1145
Bsp119I TTCGAA 1 cut(s) 1365
Bsp120I GGGCCC 1 cut(s) 1081
Bsp1286I GDGCHC 3 cut(s) 1006, 1085, 1360
Bsp13I TCCGGA 1 cut(s) 299
Bsp143I GATC 8 cut(s) 48, 64, 129, 145, 402, 484, 852, 1231
Bsp1720I GCTNAGC 1 cut(s) 1177
BspACI CCGC 6 cut(s) 20, 29, 174, 950, 1264, 1661
BspANI GGCC 1 cut(s) 1083
BspCNI CTCAG 3 cut(s) 879, 1048, 1444
BspDI ATCGAT 1 cut(s) 47
BspEI TCCGGA 1 cut(s) 299
BspFNI CGCG 2 cut(s) 124, 719
BspHI TCATGA 1 cut(s) 711
BspLI GGNNCC 8 cut(s) 241, 486, 870, 1005, 1083, 1465, 1594, 1602
BspMAI CTGCAG 2 cut(s) 493, 1194
BspMI ACCTGC 2 cut(s) 610, 1197
BspPI GGATC 3 cut(s) 479, 492, 1239
BspQI GCTCTTC 1 cut(s) 1286
BspT104I TTCGAA 1 cut(s) 1365
BspT107I GGYRCC 2 cut(s) 1003, 1600
BspTNI GGTCTC 2 cut(s) 828, 1145
BsrDI GCAATG 1 cut(s) 1004
BsrFI RCCGGY 1 cut(s) 1006
BsrI ACTGG 9 cut(s) 103, 189, 294, 349, 664, 670, 729, 1130, 1357
BssAI RCCGGY 1 cut(s) 1006
BssECI CCNNGG 5 cut(s) 243, 480, 690, 992, 1596
BssMI GATC 8 cut(s) 48, 64, 129, 145, 402, 484, 852, 1231
BssT1I CCWWGG 2 cut(s) 690, 992
Bst2UI CCWGG 5 cut(s) 284, 481, 1229, 1587, 1674
Bst4CI ACNGT 6 cut(s) 73, 815, 832, 1068, 1149, 1463
Bst6I CTCTTC 3 cut(s) 592, 1286, 1770
BstBI TTCGAA 1 cut(s) 1365
BstC8I GCNNGC 3 cut(s) 98, 224, 1023
BstDEI CTNAG 5 cut(s) 887, 1035, 1086, 1177, 1452
BstF5I GGATG 5 cut(s) 356, 367, 725, 1249, 1389
BstFNI CGCG 2 cut(s) 124, 719
BstH2I RGCGCY 1 cut(s) 103
BstHHI GCGC 3 cut(s) 102, 126, 228
BstKTI GATC 8 cut(s) 51, 67, 132, 148, 405, 487, 855, 1234
BstMAI GTCTC 3 cut(s) 672, 828, 1145
BstMBI GATC 8 cut(s) 48, 64, 129, 145, 402, 484, 852, 1231
BstMCI CGRYCG 2 cut(s) 51, 315
BstMWI GCNNNNNNNGC 7 cut(s) 26, 35, 180, 219, 497, 994, 1080
BstNI CCWGG 5 cut(s) 284, 481, 1229, 1587, 1674
BstSCI CCNGG 7 cut(s) 169, 282, 479, 1227, 1585, 1596, 1672
BstSFI CTRYAG 3 cut(s) 412, 489, 1190
BstSLI GKGCMC 2 cut(s) 1006, 1085
BstUI CGCG 2 cut(s) 124, 719
BstV1I GCAGC 8 cut(s) 4, 22, 352, 503, 1194, 1204, 1516, 1689
BstV2I GAAGAC 1 cut(s) 99
BstX2I RGATCY 1 cut(s) 484
BstXI CCANNNNNNTGG 1 cut(s) 999
BstYI RGATCY 1 cut(s) 484
Bsu15I ATCGAT 1 cut(s) 47
BsuI GTATCC 2 cut(s) 553, 1593
BsuRI GGCC 1 cut(s) 1083
BsuTUI ATCGAT 1 cut(s) 47
BtsCI GGATG 5 cut(s) 356, 367, 725, 1249, 1389
BtsI GCAGTG 1 cut(s) 327
BtsIMutI CAGTG 7 cut(s) 196, 327, 820, 1137, 1292, 1350, 1432
BveI ACCTGC 2 cut(s) 610, 1197
Cac8I GCNNGC 3 cut(s) 98, 224, 1023
CaiI CAGNNNCTG 1 cut(s) 289
CciI TCATGA 1 cut(s) 711
CfoI GCGC 3 cut(s) 102, 126, 228
Cfr10I RCCGGY 1 cut(s) 1006
Cfr13I GGNCC 6 cut(s) 247, 1031, 1081, 1082, 1592, 1658
ClaI ATCGAT 1 cut(s) 47
CseI GACGC 1 cut(s) 41
Csp6I GTAC 4 cut(s) 6, 91, 517, 1601
CspAI ACCGGT 1 cut(s) 1006
CspCI CAANNNNNGTGG 2 cut(s) 1436, 1471
CviQI GTAC 4 cut(s) 6, 91, 517, 1601
DdeI CTNAG 5 cut(s) 887, 1035, 1086, 1177, 1452
DpnI GATC 8 cut(s) 50, 66, 131, 147, 404, 486, 854, 1233
DpnII GATC 8 cut(s) 48, 64, 129, 145, 402, 484, 852, 1231
DrdI GACNNNNNNGTC 1 cut(s) 149
DseDI GACNNNNNNGTC 1 cut(s) 149
Eam1104I CTCTTC 3 cut(s) 592, 1286, 1770
EarI CTCTTC 3 cut(s) 592, 1286, 1770
EciI GGCGGA 1 cut(s) 44
Eco130I CCWWGG 2 cut(s) 690, 992
Eco24I GRGCYC 2 cut(s) 1085, 1360
Eco31I GGTCTC 2 cut(s) 828, 1145
Eco32I GATATC 1 cut(s) 160
Eco47I GGWCC 4 cut(s) 247, 1031, 1592, 1658
EcoO109I RGGNCCY 3 cut(s) 247, 1082, 1592
EcoRII CCWGG 5 cut(s) 282, 479, 1227, 1585, 1672
EcoRV GATATC 1 cut(s) 160
EcoT14I CCWWGG 2 cut(s) 690, 992
EcoT38I GRGCYC 2 cut(s) 1085, 1360
ErhI CCWWGG 2 cut(s) 690, 992
FaqI GGGAC 3 cut(s) 179, 1472, 1578
FauI CCCGC 1 cut(s) 167
FblI GTMKAC 2 cut(s) 138, 1635
Fnu4HI GCNGC 8 cut(s) 18, 36, 366, 492, 1193, 1208, 1530, 1703
FokI GGATG 5 cut(s) 363, 374, 712, 1236, 1396
FriOI GRGCYC 2 cut(s) 1085, 1360
Fsp4HI GCNGC 8 cut(s) 18, 36, 366, 492, 1193, 1208, 1530, 1703
FspBI CTAG 1 cut(s) 1653
GlaI GCGC 3 cut(s) 101, 125, 227
GluI GCNGC 8 cut(s) 18, 36, 366, 492, 1193, 1208, 1530, 1703
GsaI CCCAGC 2 cut(s) 59, 1472
GsuI CTGGAG 3 cut(s) 366, 1211, 1608
HaeII RGCGCY 1 cut(s) 103
HaeIII GGCC 1 cut(s) 1083
HapII CCGG 8 cut(s) 111, 171, 300, 315, 1007, 1109, 1598, 1692
HgaI GACGC 1 cut(s) 41
HhaI GCGC 3 cut(s) 102, 126, 228
Hin6I GCGC 3 cut(s) 100, 124, 226
HinP1I GCGC 3 cut(s) 100, 124, 226
HincII GTYRAC 4 cut(s) 76, 118, 1099, 1636
HindII GTYRAC 4 cut(s) 76, 118, 1099, 1636
HindIII AAGCTT 1 cut(s) 1088
HinfI GANTC 7 cut(s) 119, 379, 721, 749, 1376, 1667, 1734
HpaI GTTAAC 1 cut(s) 1099
HpaII CCGG 8 cut(s) 111, 171, 300, 315, 1007, 1109, 1598, 1692
HphI GGTGA 6 cut(s) 61, 397, 553, 582, 1028, 1662
Hpy166II GTNNAC 7 cut(s) 76, 118, 139, 342, 1099, 1564, 1636
Hpy188I TCNGA 9 cut(s) 12, 155, 244, 271, 312, 1038, 1145, 1261, 1453
Hpy188III TCNNGA 5 cut(s) 300, 425, 671, 712, 1750
Hpy8I GTNNAC 7 cut(s) 76, 118, 139, 342, 1099, 1564, 1636
Hpy99I CGWCG 2 cut(s) 146, 395
HpyAV CCTTC 5 cut(s) 352, 483, 619, 1273, 1708
HpyCH4III ACNGT 6 cut(s) 73, 815, 832, 1068, 1149, 1463
HpyCH4IV ACGT 1 cut(s) 1095
HpyF10VI GCNNNNNNNGC 7 cut(s) 26, 35, 180, 219, 497, 994, 1080
HpyF3I CTNAG 5 cut(s) 887, 1035, 1086, 1177, 1452
HpySE526I ACGT 1 cut(s) 1095
HspAI GCGC 3 cut(s) 100, 124, 226
Kpn2I TCCGGA 1 cut(s) 299
KpnI GGTACC 1 cut(s) 1604
KspAI GTTAAC 1 cut(s) 1099
Kzo9I GATC 8 cut(s) 48, 64, 129, 145, 402, 484, 852, 1231
LguI GCTCTTC 1 cut(s) 1286
LmnI GCTCC 2 cut(s) 245, 1321
Lsp1109I GCAGC 8 cut(s) 4, 22, 352, 503, 1194, 1204, 1516, 1689
LweI GCATC 6 cut(s) 352, 570, 749, 1067, 1204, 1770
MaeI CTAG 1 cut(s) 1653
MaeII ACGT 1 cut(s) 1095
MaeIII GTNAC 6 cut(s) 668, 750, 882, 941, 1132, 1348
MalI GATC 8 cut(s) 50, 66, 131, 147, 404, 486, 854, 1233
MboI GATC 8 cut(s) 48, 64, 129, 145, 402, 484, 852, 1231
MfeI CAATTG 2 cut(s) 954, 1171
MflI RGATCY 1 cut(s) 484
MhlI GDGCHC 3 cut(s) 1006, 1085, 1360
MlyI GAGTC 5 cut(s) 113, 373, 758, 1385, 1743
MmeI TCCRAC 4 cut(s) 335, 622, 1168, 1246
MroI TCCGGA 1 cut(s) 299
MroXI GAANNNNTTC 1 cut(s) 1295
MseI TTAA 4 cut(s) 279, 762, 927, 1098
MslI CAYNNNNRTG 1 cut(s) 903
MspI CCGG 8 cut(s) 111, 171, 300, 315, 1007, 1109, 1598, 1692
MspR9I CCNGG 7 cut(s) 171, 284, 481, 1229, 1587, 1598, 1674
MunI CAATTG 2 cut(s) 954, 1171
MvaI CCWGG 5 cut(s) 284, 481, 1229, 1587, 1674
MvnI CGCG 2 cut(s) 124, 719
MwoI GCNNNNNNNGC 7 cut(s) 26, 35, 180, 219, 497, 994, 1080
NciI CCSGG 2 cut(s) 171, 1598
NdeII GATC 8 cut(s) 48, 64, 129, 145, 402, 484, 852, 1231
NlaIV GGNNCC 8 cut(s) 241, 486, 870, 1005, 1083, 1465, 1594, 1602
NmuCI GTSAC 4 cut(s) 668, 750, 1132, 1348
NspV TTCGAA 1 cut(s) 1365
PagI TCATGA 1 cut(s) 711
PaqCI CACCTGC 1 cut(s) 1197
PciSI GCTCTTC 1 cut(s) 1286
PcsI WCGNNNNNNNCGW 2 cut(s) 69, 138
PdmI GAANNNNTTC 1 cut(s) 1295
PfeI GAWTC 2 cut(s) 721, 1667
PflMI CCANNNNNTGG 1 cut(s) 289
PfoI TCCNGGA 2 cut(s) 1227, 1585
PinAI ACCGGT 1 cut(s) 1006
PkrI GCNGC 8 cut(s) 19, 37, 367, 493, 1194, 1209, 1531, 1704
Ple19I CGATCG 1 cut(s) 51
PleI GAGTC 5 cut(s) 113, 373, 757, 1384, 1742
PpsI GAGTC 5 cut(s) 113, 373, 757, 1384, 1742
PpuMI RGGWCCY 2 cut(s) 247, 1592
Psp5II RGGWCCY 2 cut(s) 247, 1592
Psp6I CCWGG 5 cut(s) 282, 479, 1227, 1585, 1672
PspFI CCCAGC 2 cut(s) 55, 1468
PspGI CCWGG 5 cut(s) 282, 479, 1227, 1585, 1672
PspN4I GGNNCC 8 cut(s) 241, 486, 870, 1005, 1083, 1465, 1594, 1602
PspOMI GGGCCC 1 cut(s) 1081
PspPI GGNCC 6 cut(s) 247, 1031, 1081, 1082, 1592, 1658
PspPPI RGGWCCY 2 cut(s) 247, 1592
PstI CTGCAG 2 cut(s) 493, 1194
PstNI CAGNNNCTG 1 cut(s) 289
PsuI RGATCY 1 cut(s) 484
PvuI CGATCG 1 cut(s) 51
RsaI GTAC 4 cut(s) 7, 92, 518, 1602
RsaNI GTAC 4 cut(s) 6, 91, 517, 1601
RseI CAYNNNNRTG 1 cut(s) 903
SalI GTCGAC 1 cut(s) 1634
SapI GCTCTTC 1 cut(s) 1286
SaqAI TTAA 4 cut(s) 279, 762, 927, 1098
SatI GCNGC 8 cut(s) 18, 36, 366, 492, 1193, 1208, 1530, 1703
Sau3AI GATC 8 cut(s) 48, 64, 129, 145, 402, 484, 852, 1231
Sau96I GGNCC 6 cut(s) 247, 1031, 1081, 1082, 1592, 1658
ScaI AGTACT 2 cut(s) 7, 92
SchI GAGTC 5 cut(s) 113, 373, 758, 1385, 1743
ScrFI CCNGG 7 cut(s) 171, 284, 481, 1229, 1587, 1598, 1674
SduI GDGCHC 3 cut(s) 1006, 1085, 1360
SfaNI GCATC 6 cut(s) 352, 570, 749, 1067, 1204, 1770
SfcI CTRYAG 3 cut(s) 412, 489, 1190
SfuI TTCGAA 1 cut(s) 1365
SgrAI CRCCGGYG 1 cut(s) 1006
SinI GGWCC 4 cut(s) 247, 1031, 1592, 1658
SmiMI CAYNNNNRTG 1 cut(s) 903
SmlI CTYRAG 2 cut(s) 86, 435
SmoI CTYRAG 2 cut(s) 86, 435
SsiI CCGC 6 cut(s) 20, 29, 174, 950, 1264, 1661
SspMI CTAG 1 cut(s) 1653
StyD4I CCNGG 7 cut(s) 169, 282, 479, 1227, 1585, 1596, 1672
StyI CCWWGG 2 cut(s) 690, 992
TaaI ACNGT 6 cut(s) 73, 815, 832, 1068, 1149, 1463
TaiI ACGT 1 cut(s) 1098
TaqI TCGA 5 cut(s) 47, 390, 401, 1365, 1635
TatI WGTACW 2 cut(s) 5, 90
TfiI GAWTC 2 cut(s) 721, 1667
Tru1I TTAA 4 cut(s) 279, 762, 927, 1098
Tru9I TTAA 4 cut(s) 279, 762, 927, 1098
TscAI CASTG 7 cut(s) 196, 334, 820, 1137, 1292, 1357, 1432
TseFI GTSAC 4 cut(s) 668, 750, 1132, 1348
TseI GCWGC 8 cut(s) 17, 35, 365, 491, 1192, 1207, 1529, 1702
Tsp45I GTSAC 4 cut(s) 668, 750, 1132, 1348
TspDTI ATGAA 6 cut(s) 243, 728, 887, 1066, 1400, 1400
TspGWI ACGGA 1 cut(s) 534
TspRI CASTG 7 cut(s) 196, 334, 820, 1137, 1292, 1357, 1432
Van91I CCANNNNNTGG 1 cut(s) 289
VpaK11BI GGWCC 4 cut(s) 247, 1031, 1592, 1658
XapI RAATTY 4 cut(s) 306, 764, 980, 1570
XmiI GTMKAC 2 cut(s) 138, 1635
XmnI GAANNNNTTC 1 cut(s) 1295
XspI CTAG 1 cut(s) 1653
ZrmI AGTACT 2 cut(s) 7, 92
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.