Rroxscaffold_6G00397460

MACPF domain-containing protein At4g24290-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000006
OG0015525
Physical Location & Seq
Reverse (-)
19146101 .. 19152004
5904 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_6G00397460.1

Sequence Viewer

Length: 1623 bp
ATGAGTACTCGGAAAGCTGCGGCTGAGGCGGAGGCTGCGATAAAATCGATTGGGCTGGGCTACGATCTCACCGTTGACTTGAAGCTCAAGTACTGCAAGCGCCAGTCTTCCGGTGTTGACTCGCGCTTGATCGCCGTCGACGACGATCAAGTCCGAGATATCGCTATTCCCGGCGGGATTTGCATACCCACTGTCCCCAAGTCCATCAAATGCGATAAGGGCGAGCGCATGAGGTTCGGCTCCGAGGTCCTTTCATTTCAACAGATGTCAGAGCAGTTTAACCAGGAACTGGCTCTGTCCGGTAAAATTCCGACCGGACATTTCAACACTGCGTTTGAGTTTACTGGAGGATGGCAGAAGGATGCTGCCAACACAAAGACTCTTGCTTTCGACGGGGTTTCGATCACCCTCTACAGCGTTGCTCTTGACAAATCTCAGGTAGCCTTGCGTGATGATGTCAAACAAGCTGTTCCTTCTTCCTGGGATCCTGCAGCATTGGCAAGGTTTATTGAGAAGTACGGAACTCATGTTATAGTTGGGGTGAAGATGGGGGGAAAGGATACGATATACGCCAAGCAGATGCACTCTTCACCTGTCCAACCTGCTGATGTGCAGAAAAAATTGAAGGACATGGCAGACAAAATGTATATAGATGGAACTGGAACTGGTCACGAAAGAGACAAGTTCGCCAAGGAGCATGGGCAGGGTATTATGAACGCGATTCCATCCAGTTCTTACGCATCTGTTGAGTCACAGGATATTAAATTTGTGTGCAAGAGAAAGGGTGGAAGCTTACAAAAAAATCTACGCCACAGTGAGTGGTCTCAAACAGTTCAGCTTGAACCTGATGTGATCTCAATGTCATTGGTTCCTATCACATCGTTACTGAGTGGAGTTCATGGGAGTGGATTTTTGAGCCATGCCATTAACCTCTATCTTCGTTACAAACCGCCAATTGAAGAACTACATCAATTTTTGGAATTTCAGCTTCCAAGGCAATGGGCACCGGTGTTTGGTGAGCTTGCTCTTGGTCCTCAGAAGAAGCAACAAGGTGGTGCATCTTTACAGTTTAGCTTCATGGGCCCTAAGCTTTACGTTAACACAAATCCGGTTGATGTGGGGAAGAAACCAGTGACGGGTCTCCGACTGTATTTGGAGGGAAAAAGGAGCAATTGCTTAGCAATCCACCTGCAGCATCTTTCAACGCTGCCAAAGACTTTCCAACTCCAGGATCATTTGCATATCTCTCATCCCTCATCTGACCGCAGATATTATGAGAAGGTTCAGTGGAAGAGCTTCTCTCATGTCTGCACAGCTCCTATTGAGGCTAATGATGATTTCTCTGTTGTCACTGGGGCTCACTTCGAAATTGGAGAGTCGGGGATGAAAAAAGTATTATTTTTACGACTTCATTTCTCTAAAGTCAGCGGTGCAACCAATGTTAGACAATCTGAGTGGGACGGTTCCCCAGCCTTGACACAGAAATCGGGTATTATTTCAACCATTATCAGCACTCGTTTCTCGTCTGCTGCCCAAAACCAGCCCCCTAATCCTGCTGATGTGAACATAAATTCGGCTTTGTATCCTGGAGGCCCCCGGTACCCACACAGACGCCTAAACTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
MACPF

Protein Analysis

540

Amino Acids

59.58

Weight (kDa)

9.18

Isoelectric Point (pI)

42.25

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
MACPF PF01823 107 - 312 1.1e-38 MAC/Perforin domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0015525)

Species Orthologous Gene IDs
fragaria_vesca FvH4_1g25760 FvH4_1g25760
malus_domestica MD15G1140100.v1.1
prunus_persica Prupe.4G285900_v2.0.a1
pyrus_communis pycom15g12660
rosa_chinensis RchiOBHm_Chr3g0484761
rosa_laevigata RLG00000023205
rosa_multiflora Rmu_sc0001689.1_g000010 Rmu_sc0006653.1_g000005
rosa_roxburghii Rroxscaffold_6G00397460
rosa_rugosa Rorug03G0213800
rosa_samantha Rh3AG264100 Rh3BG299100 Rh3CG296900 Rh3DG292700
rosa_wichuraiana Rw3G023550

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 1197
AasI GACNNNNNNGTC 1 cut(s) 149
Acc36I ACCTGC 2 cut(s) 610, 1197
Acc65I GGTACC 1 cut(s) 1599
AccB1I GGYRCC 2 cut(s) 1003, 1599
AccB7I CCANNNNNTGG 1 cut(s) 289
AccI GTMKAC 1 cut(s) 138
AccII CGCG 2 cut(s) 124, 719
AciI CCGC 6 cut(s) 20, 29, 174, 950, 1264, 1428
AclWI GGATC 3 cut(s) 479, 492, 1239
AcsI RAATTY 4 cut(s) 306, 764, 980, 1570
AcyI GRCGYC 1 cut(s) 1612
AfaI GTAC 4 cut(s) 7, 92, 518, 1601
AfiI CCNNNNNNNGG 4 cut(s) 289, 1013, 1136, 1228
AgeI ACCGGT 1 cut(s) 1006
AgsI TTSAA 8 cut(s) 82, 260, 325, 625, 842, 959, 1203, 1500
AjnI CCWGG 4 cut(s) 282, 479, 1227, 1585
Alw26I GTCTC 3 cut(s) 672, 828, 1145
AlwI GGATC 3 cut(s) 479, 492, 1239
AlwNI CAGNNNCTG 1 cut(s) 289
AoxI GGCC 2 cut(s) 1081, 1591
ApaI GGGCCC 1 cut(s) 1085
ApeKI GCWGC 7 cut(s) 17, 35, 365, 491, 1192, 1207, 1529
ApoI RAATTY 4 cut(s) 306, 764, 980, 1570
AsiGI ACCGGT 1 cut(s) 1006
Asp700I GAANNNNTTC 1 cut(s) 1295
Asp718I GGTACC 1 cut(s) 1599
AspLEI GCGC 3 cut(s) 102, 126, 228
AspS9I GGNCC 5 cut(s) 247, 1031, 1081, 1082, 1592
AsuC2I CCSGG 2 cut(s) 171, 1597
AsuHPI GGTGA 5 cut(s) 61, 397, 553, 582, 1028
AsuII TTCGAA 1 cut(s) 1365
AvaII GGWCC 2 cut(s) 247, 1031
BaeGI GKGCMC 2 cut(s) 1006, 1085
BamHI GGATCC 1 cut(s) 484
BanI GGYRCC 2 cut(s) 1003, 1599
BanII GRGCYC 2 cut(s) 1085, 1360
BarI GAAGNNNNNNTAC 2 cut(s) 74, 106
BbsI GAAGAC 1 cut(s) 99
BbvCI CCTCAGC 1 cut(s) 24
BbvI GCAGC 7 cut(s) 4, 22, 352, 503, 1194, 1204, 1516
BccI CCATC 5 cut(s) 212, 345, 541, 647, 733
BceAI ACGGC 1 cut(s) 119
BcgI CGANNNNNNTGC 2 cut(s) 217, 251
BciT130I CCWGG 4 cut(s) 284, 481, 1229, 1587
BciVI GTATCC 2 cut(s) 553, 1593
BcnI CCSGG 2 cut(s) 171, 1597
BcoDI GTCTC 3 cut(s) 672, 828, 1145
BfmI CTRYAG 3 cut(s) 412, 489, 1190
BfoI RGCGCY 1 cut(s) 103
BfuAI ACCTGC 2 cut(s) 610, 1197
BfuI GTATCC 2 cut(s) 553, 1593
BisI GCNGC 8 cut(s) 18, 21, 36, 366, 492, 1193, 1208, 1530
BlpI GCTNAGC 1 cut(s) 1177
BlsI GCNGC 8 cut(s) 19, 22, 37, 367, 493, 1194, 1209, 1531
BmcAI AGTACT 2 cut(s) 7, 92
Bme1390I CCNGG 6 cut(s) 171, 284, 481, 1229, 1587, 1597
Bme18I GGWCC 2 cut(s) 247, 1031
BmgT120I GGNCC 5 cut(s) 247, 1031, 1081, 1082, 1592
BmiI GGNNCC 8 cut(s) 241, 486, 870, 1005, 1083, 1465, 1594, 1601
BmrFI CCNGG 6 cut(s) 171, 284, 481, 1229, 1587, 1597
BmrI ACTGGG 1 cut(s) 1362
BmsI GCATC 5 cut(s) 352, 570, 749, 1067, 1204
BmuI ACTGGG 1 cut(s) 1362
BpiI GAAGAC 1 cut(s) 99
BplI GAGNNNNNCTC 2 cut(s) 1285, 1317
BpmI CTGGAG 3 cut(s) 366, 1211, 1608
Bpu10I CCTNAGC 2 cut(s) 24, 1086
Bpu1102I GCTNAGC 1 cut(s) 1177
Bpu14I TTCGAA 1 cut(s) 1365
BpuEI CTTGAG 1 cut(s) 71
BpuMI CCSGG 2 cut(s) 171, 1597
Bsa29I ATCGAT 1 cut(s) 47
BsaHI GRCGYC 1 cut(s) 1612
BsaI GGTCTC 2 cut(s) 828, 1145
BsaJI CCNNGG 5 cut(s) 243, 480, 690, 992, 1595
BsaWI WCCGGW 5 cut(s) 110, 299, 314, 1006, 1108
Bsc4I CCNNNNNNNGG 4 cut(s) 289, 1013, 1136, 1228
Bse118I RCCGGY 1 cut(s) 1006
Bse1I ACTGG 8 cut(s) 103, 294, 349, 664, 670, 729, 1130, 1357
Bse3DI GCAATG 1 cut(s) 1004
BseBI CCWGG 4 cut(s) 284, 481, 1229, 1587
BseCI ATCGAT 1 cut(s) 47
BseDI CCNNGG 5 cut(s) 243, 480, 690, 992, 1595
BseGI GGATG 5 cut(s) 356, 367, 725, 1249, 1389
BseLI CCNNNNNNNGG 4 cut(s) 289, 1013, 1136, 1228
BseMI GCAATG 1 cut(s) 1004
BseMII CTCAG 5 cut(s) 15, 449, 878, 1049, 1443
BseNI ACTGG 8 cut(s) 103, 294, 349, 664, 670, 729, 1130, 1357
BseSI GKGCMC 2 cut(s) 1006, 1085
BseXI GCAGC 7 cut(s) 4, 22, 352, 503, 1194, 1204, 1516
BseYI CCCAGC 2 cut(s) 55, 1468
BsgI GTGCAG 2 cut(s) 632, 1294
Bsh1236I CGCG 2 cut(s) 124, 719
Bsh1285I CGRYCG 1 cut(s) 315
BshFI GGCC 2 cut(s) 1083, 1593
BshNI GGYRCC 2 cut(s) 1003, 1599
BshTI ACCGGT 1 cut(s) 1006
BshVI ATCGAT 1 cut(s) 47
BsiEI CGRYCG 1 cut(s) 315
BsiSI CCGG 7 cut(s) 111, 171, 300, 315, 1007, 1109, 1597
BslFI GGGAC 2 cut(s) 179, 1472
BslI CCNNNNNNNGG 4 cut(s) 289, 1013, 1136, 1228
BsmAI GTCTC 3 cut(s) 672, 828, 1145
BsmFI GGGAC 2 cut(s) 179, 1472
BsnI GGCC 2 cut(s) 1083, 1593
Bso31I GGTCTC 2 cut(s) 828, 1145
Bsp119I TTCGAA 1 cut(s) 1365
Bsp120I GGGCCC 1 cut(s) 1081
Bsp1286I GDGCHC 3 cut(s) 1006, 1085, 1360
Bsp143I GATC 7 cut(s) 64, 129, 145, 402, 484, 852, 1231
Bsp1720I GCTNAGC 1 cut(s) 1177
BspACI CCGC 6 cut(s) 20, 29, 174, 950, 1264, 1428
BspANI GGCC 2 cut(s) 1083, 1593
BspCNI CTCAG 5 cut(s) 16, 448, 879, 1048, 1444
BspDI ATCGAT 1 cut(s) 47
BspFNI CGCG 2 cut(s) 124, 719
BspLI GGNNCC 8 cut(s) 241, 486, 870, 1005, 1083, 1465, 1594, 1601
BspMAI CTGCAG 2 cut(s) 493, 1194
BspMI ACCTGC 2 cut(s) 610, 1197
BspPI GGATC 3 cut(s) 479, 492, 1239
BspQI GCTCTTC 1 cut(s) 1286
BspT104I TTCGAA 1 cut(s) 1365
BspT107I GGYRCC 2 cut(s) 1003, 1599
BspTNI GGTCTC 2 cut(s) 828, 1145
BsrDI GCAATG 1 cut(s) 1004
BsrFI RCCGGY 1 cut(s) 1006
BsrI ACTGG 8 cut(s) 103, 294, 349, 664, 670, 729, 1130, 1357
BssAI RCCGGY 1 cut(s) 1006
BssECI CCNNGG 5 cut(s) 243, 480, 690, 992, 1595
BssMI GATC 7 cut(s) 64, 129, 145, 402, 484, 852, 1231
BssNI GRCGYC 1 cut(s) 1612
BssT1I CCWWGG 2 cut(s) 690, 992
Bst2UI CCWGG 4 cut(s) 284, 481, 1229, 1587
Bst4CI ACNGT 7 cut(s) 73, 193, 815, 832, 1068, 1149, 1463
Bst6I CTCTTC 2 cut(s) 592, 1286
BstACI GRCGYC 1 cut(s) 1612
BstBI TTCGAA 1 cut(s) 1365
BstC8I GCNNGC 3 cut(s) 98, 224, 1023
BstDEI CTNAG 7 cut(s) 24, 435, 887, 1035, 1086, 1177, 1452
BstF5I GGATG 5 cut(s) 356, 367, 725, 1249, 1389
BstFNI CGCG 2 cut(s) 124, 719
BstH2I RGCGCY 1 cut(s) 103
BstHHI GCGC 3 cut(s) 102, 126, 228
BstKTI GATC 7 cut(s) 67, 132, 148, 405, 487, 855, 1234
BstMAI GTCTC 3 cut(s) 672, 828, 1145
BstMBI GATC 7 cut(s) 64, 129, 145, 402, 484, 852, 1231
BstMCI CGRYCG 1 cut(s) 315
BstMWI GCNNNNNNNGC 7 cut(s) 26, 35, 180, 219, 497, 994, 1080
BstNI CCWGG 4 cut(s) 284, 481, 1229, 1587
BstSCI CCNGG 6 cut(s) 169, 282, 479, 1227, 1585, 1595
BstSFI CTRYAG 3 cut(s) 412, 489, 1190
BstSLI GKGCMC 2 cut(s) 1006, 1085
BstUI CGCG 2 cut(s) 124, 719
BstV1I GCAGC 7 cut(s) 4, 22, 352, 503, 1194, 1204, 1516
BstV2I GAAGAC 1 cut(s) 99
BstX2I RGATCY 1 cut(s) 484
BstXI CCANNNNNNTGG 1 cut(s) 999
BstYI RGATCY 1 cut(s) 484
Bsu15I ATCGAT 1 cut(s) 47
BsuI GTATCC 2 cut(s) 553, 1593
BsuRI GGCC 2 cut(s) 1083, 1593
BsuTUI ATCGAT 1 cut(s) 47
BtsCI GGATG 5 cut(s) 356, 367, 725, 1249, 1389
BtsI GCAGTG 1 cut(s) 327
BtsIMutI CAGTG 6 cut(s) 189, 327, 820, 1137, 1292, 1350
BveI ACCTGC 2 cut(s) 610, 1197
Cac8I GCNNGC 3 cut(s) 98, 224, 1023
CaiI CAGNNNCTG 1 cut(s) 289
CfoI GCGC 3 cut(s) 102, 126, 228
Cfr10I RCCGGY 1 cut(s) 1006
Cfr13I GGNCC 5 cut(s) 247, 1031, 1081, 1082, 1592
ClaI ATCGAT 1 cut(s) 47
Csp6I GTAC 4 cut(s) 6, 91, 517, 1600
CspAI ACCGGT 1 cut(s) 1006
CspCI CAANNNNNGTGG 2 cut(s) 1436, 1471
CviAII CATG 8 cut(s) 229, 527, 631, 698, 899, 920, 1078, 1304
CviQI GTAC 4 cut(s) 6, 91, 517, 1600
DdeI CTNAG 7 cut(s) 24, 435, 887, 1035, 1086, 1177, 1452
DpnI GATC 7 cut(s) 66, 131, 147, 404, 486, 854, 1233
DpnII GATC 7 cut(s) 64, 129, 145, 402, 484, 852, 1231
DrdI GACNNNNNNGTC 1 cut(s) 149
DseDI GACNNNNNNGTC 1 cut(s) 149
Eam1104I CTCTTC 2 cut(s) 592, 1286
EarI CTCTTC 2 cut(s) 592, 1286
EciI GGCGGA 1 cut(s) 44
Eco130I CCWWGG 2 cut(s) 690, 992
Eco24I GRGCYC 2 cut(s) 1085, 1360
Eco31I GGTCTC 2 cut(s) 828, 1145
Eco32I GATATC 1 cut(s) 160
Eco47I GGWCC 2 cut(s) 247, 1031
EcoO109I RGGNCCY 3 cut(s) 247, 1082, 1592
EcoRII CCWGG 4 cut(s) 282, 479, 1227, 1585
EcoRV GATATC 1 cut(s) 160
EcoT14I CCWWGG 2 cut(s) 690, 992
EcoT38I GRGCYC 2 cut(s) 1085, 1360
ErhI CCWWGG 2 cut(s) 690, 992
FaeI CATG 8 cut(s) 232, 530, 634, 701, 902, 923, 1081, 1307
FaqI GGGAC 2 cut(s) 179, 1472
FatI CATG 8 cut(s) 228, 526, 630, 697, 898, 919, 1077, 1303
FauI CCCGC 1 cut(s) 167
FblI GTMKAC 1 cut(s) 138
Fnu4HI GCNGC 8 cut(s) 18, 21, 36, 366, 492, 1193, 1208, 1530
FokI GGATG 5 cut(s) 363, 374, 712, 1236, 1396
FriOI GRGCYC 2 cut(s) 1085, 1360
Fsp4HI GCNGC 8 cut(s) 18, 21, 36, 366, 492, 1193, 1208, 1530
GlaI GCGC 3 cut(s) 101, 125, 227
GluI GCNGC 8 cut(s) 18, 21, 36, 366, 492, 1193, 1208, 1530
GsaI CCCAGC 2 cut(s) 59, 1472
GsuI CTGGAG 3 cut(s) 366, 1211, 1608
HaeII RGCGCY 1 cut(s) 103
HaeIII GGCC 2 cut(s) 1083, 1593
HapII CCGG 7 cut(s) 111, 171, 300, 315, 1007, 1109, 1597
HhaI GCGC 3 cut(s) 102, 126, 228
Hin1I GRCGYC 1 cut(s) 1612
Hin1II CATG 8 cut(s) 232, 530, 634, 701, 902, 923, 1081, 1307
Hin6I GCGC 3 cut(s) 100, 124, 226
HinP1I GCGC 3 cut(s) 100, 124, 226
HincII GTYRAC 4 cut(s) 76, 118, 139, 1099
HindII GTYRAC 4 cut(s) 76, 118, 139, 1099
HindIII AAGCTT 2 cut(s) 790, 1088
HinfI GANTC 5 cut(s) 119, 379, 721, 749, 1376
HpaI GTTAAC 1 cut(s) 1099
HpaII CCGG 7 cut(s) 111, 171, 300, 315, 1007, 1109, 1597
HphI GGTGA 5 cut(s) 61, 397, 553, 582, 1028
Hpy166II GTNNAC 6 cut(s) 76, 118, 139, 342, 1099, 1564
Hpy188I TCNGA 9 cut(s) 12, 155, 244, 271, 312, 1038, 1145, 1261, 1453
Hpy188III TCNNGA 2 cut(s) 425, 671
Hpy8I GTNNAC 6 cut(s) 76, 118, 139, 342, 1099, 1564
Hpy99I CGWCG 4 cut(s) 140, 143, 146, 395
HpyAV CCTTC 4 cut(s) 352, 483, 619, 1273
HpyCH4III ACNGT 7 cut(s) 73, 193, 815, 832, 1068, 1149, 1463
HpyCH4IV ACGT 1 cut(s) 1095
HpyF10VI GCNNNNNNNGC 7 cut(s) 26, 35, 180, 219, 497, 994, 1080
HpyF3I CTNAG 7 cut(s) 24, 435, 887, 1035, 1086, 1177, 1452
HpySE526I ACGT 1 cut(s) 1095
Hsp92I GRCGYC 1 cut(s) 1612
Hsp92II CATG 8 cut(s) 232, 530, 634, 701, 902, 923, 1081, 1307
HspAI GCGC 3 cut(s) 100, 124, 226
KpnI GGTACC 1 cut(s) 1603
KspAI GTTAAC 1 cut(s) 1099
Kzo9I GATC 7 cut(s) 64, 129, 145, 402, 484, 852, 1231
LguI GCTCTTC 1 cut(s) 1286
LmnI GCTCC 4 cut(s) 245, 694, 1167, 1321
Lsp1109I GCAGC 7 cut(s) 4, 22, 352, 503, 1194, 1204, 1516
LweI GCATC 5 cut(s) 352, 570, 749, 1067, 1204
MaeII ACGT 1 cut(s) 1095
MaeIII GTNAC 6 cut(s) 668, 750, 882, 941, 1132, 1348
MalI GATC 7 cut(s) 66, 131, 147, 404, 486, 854, 1233
MboI GATC 7 cut(s) 64, 129, 145, 402, 484, 852, 1231
MboII GAAGA 9 cut(s) 99, 468, 556, 579, 929, 971, 1051, 1135, 1303
MfeI CAATTG 2 cut(s) 954, 1171
MflI RGATCY 1 cut(s) 484
MhlI GDGCHC 3 cut(s) 1006, 1085, 1360
MluCI AATT 9 cut(s) 306, 620, 764, 954, 971, 980, 1171, 1368, 1570
MlyI GAGTC 4 cut(s) 113, 373, 758, 1385
MmeI TCCRAC 4 cut(s) 335, 622, 1168, 1246
MroXI GAANNNNTTC 1 cut(s) 1295
MseI TTAA 4 cut(s) 279, 762, 927, 1098
MslI CAYNNNNRTG 1 cut(s) 903
MspA1I CMGCKG 1 cut(s) 1428
MspI CCGG 7 cut(s) 111, 171, 300, 315, 1007, 1109, 1597
MspR9I CCNGG 6 cut(s) 171, 284, 481, 1229, 1587, 1597
MunI CAATTG 2 cut(s) 954, 1171
MvaI CCWGG 4 cut(s) 284, 481, 1229, 1587
MvnI CGCG 2 cut(s) 124, 719
MwoI GCNNNNNNNGC 7 cut(s) 26, 35, 180, 219, 497, 994, 1080
NciI CCSGG 2 cut(s) 171, 1597
NdeII GATC 7 cut(s) 64, 129, 145, 402, 484, 852, 1231
NlaIII CATG 8 cut(s) 232, 530, 634, 701, 902, 923, 1081, 1307
NlaIV GGNNCC 8 cut(s) 241, 486, 870, 1005, 1083, 1465, 1594, 1601
NmuCI GTSAC 4 cut(s) 668, 750, 1132, 1348
NspV TTCGAA 1 cut(s) 1365
PaqCI CACCTGC 1 cut(s) 1197
PciSI GCTCTTC 1 cut(s) 1286
PcsI WCGNNNNNNNCGW 2 cut(s) 69, 138
PdmI GAANNNNTTC 1 cut(s) 1295
PfeI GAWTC 1 cut(s) 721
PflMI CCANNNNNTGG 1 cut(s) 289
PfoI TCCNGGA 2 cut(s) 1227, 1585
PinAI ACCGGT 1 cut(s) 1006
PkrI GCNGC 8 cut(s) 19, 22, 37, 367, 493, 1194, 1209, 1531
PleI GAGTC 4 cut(s) 113, 373, 757, 1384
PpsI GAGTC 4 cut(s) 113, 373, 757, 1384
PpuMI RGGWCCY 1 cut(s) 247
Psp5II RGGWCCY 1 cut(s) 247
Psp6I CCWGG 4 cut(s) 282, 479, 1227, 1585
PspFI CCCAGC 2 cut(s) 55, 1468
PspGI CCWGG 4 cut(s) 282, 479, 1227, 1585
PspN4I GGNNCC 8 cut(s) 241, 486, 870, 1005, 1083, 1465, 1594, 1601
PspOMI GGGCCC 1 cut(s) 1081
PspPI GGNCC 5 cut(s) 247, 1031, 1081, 1082, 1592
PspPPI RGGWCCY 1 cut(s) 247
PstI CTGCAG 2 cut(s) 493, 1194
PstNI CAGNNNCTG 1 cut(s) 289
PsuI RGATCY 1 cut(s) 484
RsaI GTAC 4 cut(s) 7, 92, 518, 1601
RsaNI GTAC 4 cut(s) 6, 91, 517, 1600
RseI CAYNNNNRTG 1 cut(s) 903
SalI GTCGAC 1 cut(s) 137
SapI GCTCTTC 1 cut(s) 1286
SaqAI TTAA 4 cut(s) 279, 762, 927, 1098
SatI GCNGC 8 cut(s) 18, 21, 36, 366, 492, 1193, 1208, 1530
Sau3AI GATC 7 cut(s) 64, 129, 145, 402, 484, 852, 1231
Sau96I GGNCC 5 cut(s) 247, 1031, 1081, 1082, 1592
ScaI AGTACT 2 cut(s) 7, 92
SchI GAGTC 4 cut(s) 113, 373, 758, 1385
ScrFI CCNGG 6 cut(s) 171, 284, 481, 1229, 1587, 1597
SduI GDGCHC 3 cut(s) 1006, 1085, 1360
SfaNI GCATC 5 cut(s) 352, 570, 749, 1067, 1204
SfcI CTRYAG 3 cut(s) 412, 489, 1190
SfuI TTCGAA 1 cut(s) 1365
SgrAI CRCCGGYG 1 cut(s) 1006
SgrDI CGTCGACG 1 cut(s) 137
SinI GGWCC 2 cut(s) 247, 1031
SmiMI CAYNNNNRTG 1 cut(s) 903
SmlI CTYRAG 1 cut(s) 86
SmoI CTYRAG 1 cut(s) 86
Sse9I AATT 9 cut(s) 306, 620, 764, 954, 971, 980, 1171, 1368, 1570
SsiI CCGC 6 cut(s) 20, 29, 174, 950, 1264, 1428
StyD4I CCNGG 6 cut(s) 169, 282, 479, 1227, 1585, 1595
StyI CCWWGG 2 cut(s) 690, 992
TaaI ACNGT 7 cut(s) 73, 193, 815, 832, 1068, 1149, 1463
TaiI ACGT 1 cut(s) 1098
TaqI TCGA 5 cut(s) 47, 138, 390, 401, 1365
TasI AATT 9 cut(s) 306, 620, 764, 954, 971, 980, 1171, 1368, 1570
TatI WGTACW 2 cut(s) 5, 90
TauI GCSGC 1 cut(s) 23
TfiI GAWTC 1 cut(s) 721
Tru1I TTAA 4 cut(s) 279, 762, 927, 1098
Tru9I TTAA 4 cut(s) 279, 762, 927, 1098
TscAI CASTG 6 cut(s) 196, 334, 820, 1137, 1292, 1357
TseFI GTSAC 4 cut(s) 668, 750, 1132, 1348
TseI GCWGC 7 cut(s) 17, 35, 365, 491, 1192, 1207, 1529
Tsp45I GTSAC 4 cut(s) 668, 750, 1132, 1348
TspDTI ATGAA 6 cut(s) 243, 728, 887, 1066, 1400, 1400
TspGWI ACGGA 1 cut(s) 534
TspRI CASTG 6 cut(s) 196, 334, 820, 1137, 1292, 1357
Van91I CCANNNNNTGG 1 cut(s) 289
VpaK11BI GGWCC 2 cut(s) 247, 1031
XapI RAATTY 4 cut(s) 306, 764, 980, 1570
XmiI GTMKAC 1 cut(s) 138
XmnI GAANNNNTTC 1 cut(s) 1295
ZrmI AGTACT 2 cut(s) 7, 92
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.