Prupe.4G285900_v2.0.a1

MACPF domain-containing protein At4g24290-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
prunus_persica
Pp04
OG0015525
Physical Location & Seq
Forward (+)
24946884 .. 24953344
6461 bp
Loading structure...
UTR
Exon/CDS
Intron
Prupe.4G285900.1

Sequence Viewer

Length: 1854 bp
ATGAGTAGTCGAACGAGCTCGAAGAAAGCAGCGGCGGAGGCGGCGGAGGCAGCGATACAGTCGATAGGGTTGGGATACGATCTGACGGTGGACTTGAAGCTCAAGTACTGCAAGAGGCAGCAGTCAGCGGTTGGGGTTGGGGTTGACTCTAGCTTGATCGCCATCGACGACGACCAAGTTCGTGAAATTGCAATTCCAGGCGCCGGTGGGCTTTGCATACCCAATGTCCCCAAATCCATCAAATGCGACAAAGGCGAGCGCATGAGGTTCGGCTCTGATGTTCTCTCCTTTCAACAGATGTCAGAGCAATTCAACCAGGAATTGACTCTGTCTGGTAAAATCCCAACTGGGCATTTCAATACTGCATTTGAGTTTACAGGAGGATGGCAGAAGGACGCTGCCAACACCAAAACACTTGCTTTTGATGGAATCTCCATCACACTCTACAGCGTTGCTCTTGAAAAATCCCAGGTTGCCTTGCGTGACCATATCAAACAAGCTGTCCCCTCATCTTGGGATCCTGCAGCACTCGCAAGGTTTATTGACAAGTACGGTACACATGTCATAGTCGGGGTGAAAATGGGCGGAAAGGATATGATATATGCCAAGCAGCAGCACTCATCACCTCTCCAACCTGCTGATGTACAGAAAAAATTGAAGGACATGGCAGATAAGATGTACATAGATGGAGCTGGTCAGTCTAGTGTGAATTCTGAAAAATTCTACGACAGAGACAAGTTGGTCAAGGAGCGCGGGCGAGCTATCATGGACACAATCCCTTCCAGTTCGTACTCATGTGTAGAGGTGCAGGATATTAAATTCATGTGCAAGAGGAAGGGTGGAAGCTTGTATAAAAATCTACCCTACAATGAGTGGTGTCACACAGTTCAGATTGAACCTGATGTGATCTCAATGTCATTGGTTCCTATTACATCGTTACTGAGTGGGATTCATGGGAGCGGATTTTTAAGTCATGCCATTAATCTCTATCTTCGTTATAAGCCGCCAATTGAAGAACTGCATCAATTTTTGGAGTTTCAACTTCCAAGGCAATGGGCACCGGTGTTTGGTGAGCTTGCGCTTGGTCCTCAGAGGAAGCAGCAAAGTAGTGCATCTTTACAGTTCAGCTTCATGGGTCCTAAGCTTTATGTTAATACAACTCCGGTTGATGTGGGTAAGAAGCCAGTGACTGGTCTCCGATTGTATTTGGAGGGAAAAAGAAGTAACTGCTTAGCAATCCACTTGCAGCATCTTTCTTCGCTGCCAAAGACCTTCCTACTCCAGGATGAAGTGAATGGCAATATTTCTCATCTTTCATCTGACCGGAGATATTACGAAAAGGTTCAATGGAAGAGCTTCTCTCATGTATGTACAGCTCCTGTGGAGGCTAATGATGATTTCTCTGTTGTCACTGGGGCTCACTTTGAAGTTGGAGACTCTGGCCTGAAGAAAGTTTTATTTTTACGGCTTCTCTTTTCTAAAGTTACTGGTGCAACTAATGTTAGACAAGCTGAGTGGGATGGTTCCCCTGGCTTGGCACAGAAATCTGGATTAATTTCGACCCTTATCAGCACGCGTTTCTCGAATGCTCAGAAACCACCTCCTCGGCCTTCTGATGTGAACATAAACTCCGCTGTGTATCCCGGGGGCCCTCCAGTGCCCACACAGACACCTAAACTTTTGAGGTTTGTTGACACAACAGAAATGACTAGAGGACCACAGGATTCACCTGGGTATTGGGTTGTGTCGGGGGCAAGGCTTATGGCTGAGAAGGGTAAAATATCTCTACGAGTCAAGTATTCTCTCTTGACCATGATTTTACCTGATGAAGAAGAGATGCCAGATAATTATTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
MACPF

Protein Analysis

618

Amino Acids

68.1

Weight (kDa)

8.87

Isoelectric Point (pI)

42.93

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0015525)

Species Orthologous Gene IDs
fragaria_vesca FvH4_1g25760 FvH4_1g25760
malus_domestica MD15G1140100.v1.1
prunus_persica Prupe.4G285900_v2.0.a1
pyrus_communis pycom15g12660
rosa_chinensis RchiOBHm_Chr3g0484761
rosa_laevigata RLG00000023205
rosa_multiflora Rmu_sc0001689.1_g000010 Rmu_sc0006653.1_g000005
rosa_roxburghii Rroxscaffold_6G00397460
rosa_rugosa Rorug03G0213800
rosa_samantha Rh3AG264100 Rh3BG299100 Rh3CG296900 Rh3DG292700
rosa_wichuraiana Rw3G023550

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 999
AasI GACNNNNNNGTC 1 cut(s) 740
Acc36I ACCTGC 1 cut(s) 643
AccB1I GGYRCC 2 cut(s) 200, 1057
AccB7I CCANNNNNTGG 1 cut(s) 1190
AccBSI CCGCTC 1 cut(s) 960
AccII CGCG 2 cut(s) 753, 1576
AclWI GGATC 2 cut(s) 512, 525
AcsI RAATTY 3 cut(s) 709, 719, 818
AcuI CTGAAG 1 cut(s) 1466
AcyI GRCGYC 1 cut(s) 201
AfaI GTAC 7 cut(s) 107, 551, 556, 645, 680, 791, 1372
AfiI CCNNNNNNNGG 6 cut(s) 203, 513, 1067, 1190, 1282, 1534
AflIII ACRYGT 2 cut(s) 559, 1574
AgeI ACCGGT 1 cut(s) 1060
AjnI CCWGG 6 cut(s) 196, 315, 468, 1281, 1528, 1729
AloI GAACNNNNNNTCC 2 cut(s) 1613, 1645
Alw21I GWGCWC 1 cut(s) 20
Alw26I GTCTC 3 cut(s) 726, 1199, 1428
AlwI GGATC 2 cut(s) 512, 525
AlwNI CAGNNNCTG 2 cut(s) 1190, 1379
Ama87I CYCGRG 1 cut(s) 1643
AoxI GGCC 3 cut(s) 1441, 1607, 1648
ApaI GGGCCC 1 cut(s) 1652
ApoI RAATTY 3 cut(s) 709, 719, 818
ArsI GACNNNNNNTTYG 2 cut(s) 486, 518
AseI ATTAAT 2 cut(s) 981, 1553
AsiGI ACCGGT 1 cut(s) 1060
Asp700I GAANNNNTTC 2 cut(s) 1341, 1355
AspLEI GCGC 4 cut(s) 203, 261, 753, 1081
AspS9I GGNCC 5 cut(s) 1085, 1136, 1648, 1649, 1715
AsuC2I CCSGG 2 cut(s) 1644, 1645
AsuHPI GGTGA 4 cut(s) 586, 615, 1082, 1719
AvaI CYCGRG 1 cut(s) 1643
AvaII GGWCC 3 cut(s) 1085, 1136, 1715
BaeGI GKGCMC 3 cut(s) 1060, 1652, 1662
BaeI ACNNNNGTAYC 2 cut(s) 546, 579
BamHI GGATCC 1 cut(s) 517
BanI GGYRCC 2 cut(s) 200, 1057
BanII GRGCYC 3 cut(s) 20, 1420, 1652
BarI GAAGNNNNNNTAC 2 cut(s) 89, 121
Bbv12I GWGCWC 1 cut(s) 20
BccI CCATC 7 cut(s) 170, 245, 378, 419, 443, 680, 1514
BceAI ACGGC 1 cut(s) 1481
BcgI CGANNNNNNTGC 2 cut(s) 250, 284
BciT130I CCWGG 6 cut(s) 198, 317, 470, 1283, 1530, 1731
BciVI GTATCC 2 cut(s) 68, 1650
BcnI CCSGG 2 cut(s) 1644, 1645
BcoDI GTCTC 3 cut(s) 726, 1199, 1428
BfaI CTAG 3 cut(s) 150, 702, 1710
BfmI CTRYAG 2 cut(s) 445, 522
BfoI RGCGCY 1 cut(s) 204
BfuAI ACCTGC 1 cut(s) 643
BfuI GTATCC 2 cut(s) 68, 1650
BlpI GCTNAGC 1 cut(s) 1231
BmcAI AGTACT 1 cut(s) 107
Bme1390I CCNGG 8 cut(s) 198, 317, 470, 1283, 1530, 1644, 1645, 1731
Bme18I GGWCC 3 cut(s) 1085, 1136, 1715
BmeT110I CYCGRG 1 cut(s) 1643
BmgT120I GGNCC 5 cut(s) 1085, 1136, 1648, 1649, 1715
BmiI GGNNCC 8 cut(s) 202, 519, 924, 1059, 1137, 1525, 1649, 1650
BmrFI CCNGG 8 cut(s) 198, 317, 470, 1283, 1530, 1644, 1645, 1731
BmrI ACTGGG 2 cut(s) 357, 1422
BmsI GCATC 4 cut(s) 1030, 1121, 1258, 1827
BmuI ACTGGG 2 cut(s) 357, 1422
BplI GAGNNNNNCTC 2 cut(s) 1345, 1377
BpmI CTGGAG 2 cut(s) 1265, 1638
Bpu10I CCTNAGC 1 cut(s) 1140
Bpu1102I GCTNAGC 1 cut(s) 1231
BpuEI CTTGAG 1 cut(s) 86
BpuMI CCSGG 2 cut(s) 1644, 1645
BsaBI GATNNNNATC 1 cut(s) 161
BsaHI GRCGYC 1 cut(s) 201
BsaI GGTCTC 1 cut(s) 1199
BsaJI CCNNGG 7 cut(s) 468, 1046, 1528, 1604, 1643, 1644, 1730
BsaWI WCCGGW 3 cut(s) 1060, 1162, 1323
BsaXI ACNNNNNCTCC 2 cut(s) 1613, 1643
Bsc4I CCNNNNNNNGG 6 cut(s) 203, 513, 1067, 1190, 1282, 1534
Bse118I RCCGGY 2 cut(s) 203, 1060
Bse1I ACTGG 7 cut(s) 352, 783, 1184, 1195, 1417, 1492, 1655
Bse3DI GCAATG 1 cut(s) 1058
Bse8I GATNNNNATC 1 cut(s) 161
BseBI CCWGG 6 cut(s) 198, 317, 470, 1283, 1530, 1731
BseDI CCNNGG 7 cut(s) 468, 1046, 1528, 1604, 1643, 1644, 1730
BseGI GGATG 3 cut(s) 389, 1291, 1525
BseJI GATNNNNATC 1 cut(s) 161
BseLI CCNNNNNNNGG 6 cut(s) 203, 513, 1067, 1190, 1282, 1534
BseMI GCAATG 1 cut(s) 1058
BseMII CTCAG 5 cut(s) 932, 1103, 1503, 1604, 1758
BseNI ACTGG 7 cut(s) 352, 783, 1184, 1195, 1417, 1492, 1655
BseRI GAGGAG 1 cut(s) 1593
BseSI GKGCMC 3 cut(s) 1060, 1652, 1662
BsgI GTGCAG 1 cut(s) 827
Bsh1236I CGCG 2 cut(s) 753, 1576
BshFI GGCC 3 cut(s) 1443, 1609, 1650
BshNI GGYRCC 2 cut(s) 200, 1057
BshTI ACCGGT 1 cut(s) 1060
BsiHKAI GWGCWC 1 cut(s) 20
BsiHKCI CYCGRG 1 cut(s) 1643
BsiSI CCGG 5 cut(s) 204, 1061, 1163, 1324, 1644
BslFI GGGAC 2 cut(s) 212, 488
BslI CCNNNNNNNGG 6 cut(s) 203, 513, 1067, 1190, 1282, 1534
BsmAI GTCTC 3 cut(s) 726, 1199, 1428
BsmFI GGGAC 2 cut(s) 212, 488
BsmI GAATGC 1 cut(s) 1591
BsnI GGCC 3 cut(s) 1443, 1609, 1650
Bso31I GGTCTC 1 cut(s) 1199
BsoBI CYCGRG 1 cut(s) 1643
Bsp120I GGGCCC 1 cut(s) 1648
Bsp1286I GDGCHC 5 cut(s) 20, 1060, 1420, 1652, 1662
Bsp1407I TGTACA 3 cut(s) 643, 678, 1370
Bsp143I GATC 4 cut(s) 79, 156, 517, 906
Bsp1720I GCTNAGC 1 cut(s) 1231
BspANI GGCC 3 cut(s) 1443, 1609, 1650
BspCNI CTCAG 5 cut(s) 933, 1102, 1504, 1603, 1759
BspFNI CGCG 2 cut(s) 753, 1576
BspLI GGNNCC 8 cut(s) 202, 519, 924, 1059, 1137, 1525, 1649, 1650
BspMAI CTGCAG 1 cut(s) 526
BspMI ACCTGC 1 cut(s) 643
BspPI GGATC 2 cut(s) 512, 525
BspQI GCTCTTC 1 cut(s) 1346
BspT107I GGYRCC 2 cut(s) 200, 1057
BspTNI GGTCTC 1 cut(s) 1199
BsrBI CCGCTC 1 cut(s) 960
BsrDI GCAATG 1 cut(s) 1058
BsrFI RCCGGY 2 cut(s) 203, 1060
BsrGI TGTACA 3 cut(s) 643, 678, 1370
BsrI ACTGG 7 cut(s) 352, 783, 1184, 1195, 1417, 1492, 1655
BssAI RCCGGY 2 cut(s) 203, 1060
BssECI CCNNGG 7 cut(s) 468, 1046, 1528, 1604, 1643, 1644, 1730
BssMI GATC 4 cut(s) 79, 156, 517, 906
BssNI GRCGYC 1 cut(s) 201
BssT1I CCWWGG 1 cut(s) 1046
Bst2UI CCWGG 6 cut(s) 198, 317, 470, 1283, 1530, 1731
Bst4CI ACNGT 5 cut(s) 60, 88, 554, 886, 1122
Bst6I CTCTTC 2 cut(s) 1346, 1827
BstACI GRCGYC 1 cut(s) 201
BstAUI TGTACA 3 cut(s) 643, 678, 1370
BstC8I GCNNGC 5 cut(s) 257, 755, 759, 1077, 1574
BstDEI CTNAG 7 cut(s) 941, 1089, 1140, 1231, 1512, 1590, 1767
BstENI CCTNNNNNAGG 1 cut(s) 1280
BstF5I GGATG 3 cut(s) 389, 1291, 1525
BstFNI CGCG 2 cut(s) 753, 1576
BstH2I RGCGCY 1 cut(s) 204
BstHHI GCGC 4 cut(s) 203, 261, 753, 1081
BstKTI GATC 4 cut(s) 82, 159, 520, 909
BstMAI GTCTC 3 cut(s) 726, 1199, 1428
BstMBI GATC 4 cut(s) 79, 156, 517, 906
BstMWI GCNNNNNNNGC 6 cut(s) 38, 41, 47, 50, 252, 530
BstNI CCWGG 6 cut(s) 198, 317, 470, 1283, 1530, 1731
BstNSI RCATGY 1 cut(s) 563
BstSCI CCNGG 8 cut(s) 196, 315, 468, 1281, 1528, 1642, 1643, 1729
BstSFI CTRYAG 2 cut(s) 445, 522
BstSLI GKGCMC 3 cut(s) 1060, 1652, 1662
BstUI CGCG 2 cut(s) 753, 1576
BstX2I RGATCY 1 cut(s) 517
BstXI CCANNNNNNTGG 1 cut(s) 1053
BstYI RGATCY 1 cut(s) 517
BsuI GTATCC 2 cut(s) 68, 1650
BsuRI GGCC 3 cut(s) 1443, 1609, 1650
BtsCI GGATG 3 cut(s) 389, 1291, 1525
BtsIMutI CAGTG 3 cut(s) 1191, 1410, 1662
BveI ACCTGC 1 cut(s) 643
Cac8I GCNNGC 5 cut(s) 257, 755, 759, 1077, 1574
CaiI CAGNNNCTG 2 cut(s) 1190, 1379
CfoI GCGC 4 cut(s) 203, 261, 753, 1081
Cfr10I RCCGGY 2 cut(s) 203, 1060
Cfr13I GGNCC 5 cut(s) 1085, 1136, 1648, 1649, 1715
Cfr9I CCCGGG 1 cut(s) 1643
CseI GACGC 1 cut(s) 404
Csp6I GTAC 7 cut(s) 106, 550, 555, 644, 679, 790, 1371
CspAI ACCGGT 1 cut(s) 1060
CspCI CAANNNNNGTGG 2 cut(s) 1496, 1531
CviQI GTAC 7 cut(s) 106, 550, 555, 644, 679, 790, 1371
DdeI CTNAG 7 cut(s) 941, 1089, 1140, 1231, 1512, 1590, 1767
DinI GGCGCC 1 cut(s) 202
DpnI GATC 4 cut(s) 81, 158, 519, 908
DpnII GATC 4 cut(s) 79, 156, 517, 906
DrdI GACNNNNNNGTC 1 cut(s) 740
DseDI GACNNNNNNGTC 1 cut(s) 740
Eam1104I CTCTTC 2 cut(s) 1346, 1827
EarI CTCTTC 2 cut(s) 1346, 1827
EciI GGCGGA 3 cut(s) 50, 59, 600
Ecl136II GAGCTC 1 cut(s) 18
Eco130I CCWWGG 1 cut(s) 1046
Eco24I GRGCYC 3 cut(s) 20, 1420, 1652
Eco31I GGTCTC 1 cut(s) 1199
Eco47I GGWCC 3 cut(s) 1085, 1136, 1715
Eco53kI GAGCTC 1 cut(s) 18
Eco57I CTGAAG 1 cut(s) 1466
Eco88I CYCGRG 1 cut(s) 1643
EcoICRI GAGCTC 1 cut(s) 18
EcoNI CCTNNNNNAGG 1 cut(s) 1280
EcoO109I RGGNCCY 3 cut(s) 1136, 1648, 1649
EcoRI GAATTC 1 cut(s) 709
EcoRII CCWGG 6 cut(s) 196, 315, 468, 1281, 1528, 1729
EcoT14I CCWWGG 1 cut(s) 1046
EcoT38I GRGCYC 3 cut(s) 20, 1420, 1652
EgeI GGCGCC 1 cut(s) 202
EheI GGCGCC 1 cut(s) 202
ErhI CCWWGG 1 cut(s) 1046
FaqI GGGAC 2 cut(s) 212, 488
FauI CCCGC 1 cut(s) 746
FokI GGATG 3 cut(s) 396, 1298, 1532
FriOI GRGCYC 3 cut(s) 20, 1420, 1652
FspBI CTAG 3 cut(s) 150, 702, 1710
GlaI GCGC 4 cut(s) 202, 260, 752, 1080
GsuI CTGGAG 2 cut(s) 1265, 1638
HaeII RGCGCY 1 cut(s) 204
HaeIII GGCC 3 cut(s) 1443, 1609, 1650
HapII CCGG 5 cut(s) 204, 1061, 1163, 1324, 1644
HgaI GACGC 1 cut(s) 404
HhaI GCGC 4 cut(s) 203, 261, 753, 1081
Hin1I GRCGYC 1 cut(s) 201
Hin6I GCGC 4 cut(s) 201, 259, 751, 1079
HinP1I GCGC 4 cut(s) 201, 259, 751, 1079
HincII GTYRAC 2 cut(s) 145, 1693
HindII GTYRAC 2 cut(s) 145, 1693
HindIII AAGCTT 2 cut(s) 844, 1142
HinfI GANTC 7 cut(s) 146, 325, 429, 949, 1436, 1724, 1791
HpaII CCGG 5 cut(s) 204, 1061, 1163, 1324, 1644
HphI GGTGA 4 cut(s) 586, 615, 1082, 1719
Hpy166II GTNNAC 6 cut(s) 91, 145, 375, 557, 1621, 1693
Hpy188III TCNNGA 5 cut(s) 182, 458, 1548, 1582, 1807
Hpy8I GTNNAC 6 cut(s) 91, 145, 375, 557, 1621, 1693
Hpy99I CGWCG 2 cut(s) 170, 173
HpyAV CCTTC 7 cut(s) 385, 652, 789, 829, 1282, 1620, 1765
HpyCH4III ACNGT 5 cut(s) 60, 88, 554, 886, 1122
HpyF10VI GCNNNNNNNGC 6 cut(s) 38, 41, 47, 50, 252, 530
HpyF3I CTNAG 7 cut(s) 941, 1089, 1140, 1231, 1512, 1590, 1767
Hsp92I GRCGYC 1 cut(s) 201
HspAI GCGC 4 cut(s) 201, 259, 751, 1079
KasI GGCGCC 1 cut(s) 200
Kzo9I GATC 4 cut(s) 79, 156, 517, 906
LguI GCTCTTC 1 cut(s) 1346
LmnI GCTCC 4 cut(s) 689, 748, 957, 1381
LweI GCATC 4 cut(s) 1030, 1121, 1258, 1827
MaeI CTAG 3 cut(s) 150, 702, 1710
MaeIII GTNAC 7 cut(s) 482, 878, 936, 1186, 1223, 1408, 1483
MalI GATC 4 cut(s) 81, 158, 519, 908
MbiI CCGCTC 1 cut(s) 960
MboI GATC 4 cut(s) 79, 156, 517, 906
MboII GAAGA 8 cut(s) 34, 983, 1025, 1248, 1363, 1459, 1841, 1844
MfeI CAATTG 1 cut(s) 1008
MflI RGATCY 1 cut(s) 517
MhlI GDGCHC 5 cut(s) 20, 1060, 1420, 1652, 1662
MluI ACGCGT 1 cut(s) 1574
Mly113I GGCGCC 1 cut(s) 201
MlyI GAGTC 4 cut(s) 140, 319, 1430, 1800
MmeI TCCRAC 2 cut(s) 655, 1411
MroXI GAANNNNTTC 2 cut(s) 1341, 1355
MseI TTAA 5 cut(s) 816, 968, 981, 1152, 1553
MspA1I CMGCKG 3 cut(s) 32, 128, 1634
MspI CCGG 5 cut(s) 204, 1061, 1163, 1324, 1644
MspR9I CCNGG 8 cut(s) 198, 317, 470, 1283, 1530, 1644, 1645, 1731
MunI CAATTG 1 cut(s) 1008
Mva1269I GAATGC 1 cut(s) 1591
MvaI CCWGG 6 cut(s) 198, 317, 470, 1283, 1530, 1731
MvnI CGCG 2 cut(s) 753, 1576
MwoI GCNNNNNNNGC 6 cut(s) 38, 41, 47, 50, 252, 530
NarI GGCGCC 1 cut(s) 201
NciI CCSGG 2 cut(s) 1644, 1645
NdeII GATC 4 cut(s) 79, 156, 517, 906
NlaIV GGNNCC 8 cut(s) 202, 519, 924, 1059, 1137, 1525, 1649, 1650
NmeAIII GCCGAG 1 cut(s) 1585
NmuCI GTSAC 4 cut(s) 482, 878, 1186, 1408
NspI RCATGY 1 cut(s) 563
PciI ACATGT 1 cut(s) 559
PciSI GCTCTTC 1 cut(s) 1346
PcsI WCGNNNNNNNCGW 2 cut(s) 165, 1580
PctI GAATGC 1 cut(s) 1591
PdmI GAANNNNTTC 2 cut(s) 1341, 1355
PfeI GAWTC 3 cut(s) 429, 949, 1724
PflFI GACNNNGTC 1 cut(s) 328
PflMI CCANNNNNTGG 1 cut(s) 1190
PfoI TCCNGGA 1 cut(s) 1281
PinAI ACCGGT 1 cut(s) 1060
PleI GAGTC 4 cut(s) 140, 319, 1430, 1799
PluTI GGCGCC 1 cut(s) 204
PpsI GAGTC 4 cut(s) 140, 319, 1430, 1799
PpuMI RGGWCCY 1 cut(s) 1136
PscI ACATGT 1 cut(s) 559
PshBI ATTAAT 2 cut(s) 981, 1553
PsiI TTATAA 1 cut(s) 999
Psp124BI GAGCTC 1 cut(s) 20
Psp5II RGGWCCY 1 cut(s) 1136
Psp6I CCWGG 6 cut(s) 196, 315, 468, 1281, 1528, 1729
PspGI CCWGG 6 cut(s) 196, 315, 468, 1281, 1528, 1729
PspN4I GGNNCC 8 cut(s) 202, 519, 924, 1059, 1137, 1525, 1649, 1650
PspOMI GGGCCC 1 cut(s) 1648
PspPI GGNCC 5 cut(s) 1085, 1136, 1648, 1649, 1715
PspPPI RGGWCCY 1 cut(s) 1136
PstI CTGCAG 1 cut(s) 526
PstNI CAGNNNCTG 2 cut(s) 1190, 1379
PsuI RGATCY 1 cut(s) 517
PsyI GACNNNGTC 1 cut(s) 328
RsaI GTAC 7 cut(s) 107, 551, 556, 645, 680, 791, 1372
RsaNI GTAC 7 cut(s) 106, 550, 555, 644, 679, 790, 1371
SacI GAGCTC 1 cut(s) 20
SapI GCTCTTC 1 cut(s) 1346
SaqAI TTAA 5 cut(s) 816, 968, 981, 1152, 1553
Sau3AI GATC 4 cut(s) 79, 156, 517, 906
Sau96I GGNCC 5 cut(s) 1085, 1136, 1648, 1649, 1715
ScaI AGTACT 1 cut(s) 107
SchI GAGTC 4 cut(s) 140, 319, 1430, 1800
ScrFI CCNGG 8 cut(s) 198, 317, 470, 1283, 1530, 1644, 1645, 1731
SduI GDGCHC 5 cut(s) 20, 1060, 1420, 1652, 1662
SfaNI GCATC 4 cut(s) 1030, 1121, 1258, 1827
SfcI CTRYAG 2 cut(s) 445, 522
SfoI GGCGCC 1 cut(s) 202
SgrAI CRCCGGYG 2 cut(s) 203, 1060
SinI GGWCC 3 cut(s) 1085, 1136, 1715
SmaI CCCGGG 1 cut(s) 1645
SmlI CTYRAG 1 cut(s) 101
SmoI CTYRAG 1 cut(s) 101
SspDI GGCGCC 1 cut(s) 200
SspI AATATT 1 cut(s) 1303
SspMI CTAG 3 cut(s) 150, 702, 1710
SstI GAGCTC 1 cut(s) 20
StyD4I CCNGG 8 cut(s) 196, 315, 468, 1281, 1528, 1642, 1643, 1729
StyI CCWWGG 1 cut(s) 1046
TaaI ACNGT 5 cut(s) 60, 88, 554, 886, 1122
TaqI TCGA 6 cut(s) 10, 20, 62, 165, 1559, 1583
TatI WGTACW 4 cut(s) 105, 643, 678, 1370
TauI GCSGC 3 cut(s) 35, 44, 1006
TfiI GAWTC 3 cut(s) 429, 949, 1724
Tru1I TTAA 5 cut(s) 816, 968, 981, 1152, 1553
Tru9I TTAA 5 cut(s) 816, 968, 981, 1152, 1553
TscAI CASTG 3 cut(s) 1191, 1417, 1662
TseFI GTSAC 4 cut(s) 482, 878, 1186, 1408
Tsp45I GTSAC 4 cut(s) 482, 878, 1186, 1408
TspDTI ATGAA 6 cut(s) 811, 941, 1120, 1302, 1305, 1842
TspMI CCCGGG 1 cut(s) 1643
TspRI CASTG 3 cut(s) 1191, 1417, 1662
Tth111I GACNNNGTC 1 cut(s) 328
Van91I CCANNNNNTGG 1 cut(s) 1190
VpaK11BI GGWCC 3 cut(s) 1085, 1136, 1715
VspI ATTAAT 2 cut(s) 981, 1553
XagI CCTNNNNNAGG 1 cut(s) 1280
XapI RAATTY 3 cut(s) 709, 719, 818
XceI RCATGY 1 cut(s) 563
XmaI CCCGGG 1 cut(s) 1643
XmnI GAANNNNTTC 2 cut(s) 1341, 1355
XspI CTAG 3 cut(s) 150, 702, 1710
ZrmI AGTACT 1 cut(s) 107
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.