FvH4_1g29960

Putative S-adenosyl-L-methionine-dependent methyltransferase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb1
OG0017593
Physical Location & Seq
Forward (+)
23710051 .. 23711193
1143 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_1g29960.t1

Sequence Viewer

Length: 1143 bp
ATGAAGCACAAATCCATGGATTCCCCTCATAATAAGTTCAAGTCTTTCACCTCGAATAACCTTTACTTCCTTCTCCTTCTCCTCTTGACAAATTTCATCACCTTGTTCGTTTCCTCATCCTTTTCCTCCTGTTCTTACAAACCCCTCTCGACCACCACAGCATCCGACCTCCCACCGGAGTTCCTTGCCTTCGCATCCGGACACGCTCTCCCATTCGGATACAACGCTAACTTTGATTCCGACACCATTTACCCTCCAGCTGGCAAAGCATGCACGCTCTTCCCGGACGAGCTTCGTCGCTACATGTCATACAAAGTCAATGGTTCGTGCCCTGACGACGAGCTCTTAGCCCAAAAGCTCCTCCTCAAGGGCTGCGAGCCCTTGCCCCGGCGCCGGTGCCGCCCGGCCTCTCAACCGGAGTACACTGATCCTTACCCTCTCCCCACCAGCCTATGGACTACGCCGTCCGACTCTTCAGTGGTGTGGACAGCCTACAGATGCAAGAACTACAACTGCCTCGTCAACAGAAAACGAAACGAGAAAGGCTTCACCGACTGCAAAGACTGCTTCGACCTCCGAGGAAACGAGAAGTCTCGCTGGGCAAGCCCAAAAGGAGGAGGCCTCCATTTCAGCATAGACGAAGTGTTGGCCACCAAGCCACGCGGCAGCATTCGGATCGGCCTCGACGTTGGCGGCGGGGTGGCCACGTTCGCCGTGAGAATGAGAGAGAGGAACATAACCATAGTAACGACGTCGATGAACTTGAACGGCCCTTTCAACAACTTCATAGCGTCGAGAGGTGTGATTCCTCTCTACATCAGTATTTCGCAGAGGCTTCCGTTCTTTGACAACACTTTGGACATCGTACACTCCATGCACGTTCTCAGTAACTGGATTCCGACGACGTTGCTTCATTTTGCTTTCTTTGATGTGTACAGAGTGCTTAGGCCTGGGGGTTTGTTCTGGCTCGACCATTTTTTCTGCGTGGGTGATCAATTGGAGGTGACTTATAAGCCGTTGATCGAGAGCATTGGATTCAACAAGTTGAAGTGGGTTGTTGGGAGGAAGCTTGATCGAGGCCCTGAGCTTAGGGAGATGTACCTGTCGGCTTTGTTGGAGAAGCCATTGAAGAACTCGTGGTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Methyltransf_11 Methyltransf_29

Protein Analysis

381

Amino Acids

43.12

Weight (kDa)

9.13

Isoelectric Point (pI)

42.09

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Methyltransf_29 PF03141 168 - 322 5.9e-09 Putative S-adenosyl-L-methionine-dependent methyltransferase
Methyltransf_11 PF08241 228 - 322 1.8e-06 Methyltransferase domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0017593)

Species Orthologous Gene IDs
fragaria_vesca FvH4_1g29960
malus_domestica MD17G1262900.v1.1
prunus_persica Prupe.3G164600_v2.0.a1
rosa_chinensis RchiOBHm_Chr3g0492371
rosa_laevigata RLG00000022779
rosa_multiflora Rmu_sc0003156.1_g000020
rosa_roxburghii Rroxscaffold_6G00392380
rosa_samantha Rh3AG303400 Rh3BG339600 Rh3CG336600 Rh3DG338700
rosa_wichuraiana Rw3G026770

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 1011
AasI GACNNNNNNGTC 1 cut(s) 463
AatII GACGTC 1 cut(s) 755
AccB1I GGYRCC 2 cut(s) 390, 396
AccB7I CCANNNNNTGG 1 cut(s) 453
AccII CGCG 1 cut(s) 663
AccIII TCCGGA 1 cut(s) 197
AciI CCGC 4 cut(s) 400, 663, 693, 696
AclWI GGATC 2 cut(s) 422, 683
AcoI YGGCCR 2 cut(s) 648, 702
AcsI RAATTY 1 cut(s) 91
AcuI CTGAAG 1 cut(s) 459
AcyI GRCGYC 2 cut(s) 391, 752
AfaI GTAC 4 cut(s) 422, 867, 935, 1100
AfiI CCNNNNNNNGG 7 cut(s) 175, 260, 367, 387, 393, 453, 614
AflIII ACRYGT 1 cut(s) 303
AgsI TTSAA 6 cut(s) 40, 766, 778, 1039, 1048, 1129
AjnI CCWGG 1 cut(s) 949
AluBI AGCT 6 cut(s) 260, 292, 343, 358, 1069, 1087
AluI AGCT 6 cut(s) 260, 292, 343, 358, 1069, 1087
Alw21I GWGCWC 1 cut(s) 345
Alw26I GTCTC 1 cut(s) 597
AlwI GGATC 2 cut(s) 422, 683
AlwNI CAGNNNCTG 1 cut(s) 891
Aor13HI TCCGGA 1 cut(s) 197
AoxI GGCC 8 cut(s) 405, 619, 648, 679, 702, 769, 947, 1078
ApeKI GCWGC 2 cut(s) 372, 666
ApoI RAATTY 1 cut(s) 91
Asp700I GAANNNNTTC 1 cut(s) 545
AspLEI GCGC 1 cut(s) 393
AspS9I GGNCC 2 cut(s) 770, 1079
AsuC2I CCSGG 3 cut(s) 284, 388, 404
AsuHPI GGTGA 5 cut(s) 40, 91, 541, 1001, 1015
BaeGI GKGCMC 1 cut(s) 332
BalI TGGCCA 2 cut(s) 650, 704
BanI GGYRCC 2 cut(s) 390, 396
BanII GRGCYC 2 cut(s) 345, 381
BauI CACGAG 1 cut(s) 1135
Bbv12I GWGCWC 1 cut(s) 345
BbvI GCAGC 2 cut(s) 359, 678
BceAI ACGGC 4 cut(s) 448, 698, 784, 1000
BcgI CGANNNNNNTGC 4 cut(s) 658, 692, 889, 923
BciT130I CCWGG 1 cut(s) 951
BciVI GTATCC 1 cut(s) 212
BclI TGATCA 1 cut(s) 991
BcnI CCSGG 3 cut(s) 284, 388, 404
BcoDI GTCTC 1 cut(s) 597
BfmI CTRYAG 1 cut(s) 493
BfoI RGCGCY 1 cut(s) 394
BfuI GTATCC 1 cut(s) 212
BisI GCNGC 5 cut(s) 373, 400, 664, 667, 694
BlsI GCNGC 5 cut(s) 374, 401, 665, 668, 695
Bme1390I CCNGG 4 cut(s) 284, 388, 404, 951
BmgT120I GGNCC 2 cut(s) 770, 1079
BmiI GGNNCC 2 cut(s) 392, 398
BmrFI CCNGG 4 cut(s) 284, 388, 404, 951
BmsI GCATC 3 cut(s) 170, 203, 488
BplI GAGNNNNNCTC 2 cut(s) 606, 638
BpmI CTGGAG 1 cut(s) 240
Bpu10I CCTNAGC 3 cut(s) 944, 1083, 1088
BpuEI CTTGAG 1 cut(s) 350
BpuMI CCSGG 3 cut(s) 284, 388, 404
BsaHI GRCGYC 2 cut(s) 391, 752
BsaJI CCNNGG 4 cut(s) 15, 386, 577, 950
BsaWI WCCGGW 3 cut(s) 175, 197, 415
BsaXI ACNNNNNCTCC 2 cut(s) 192, 222
Bsc4I CCNNNNNNNGG 7 cut(s) 175, 260, 367, 387, 393, 453, 614
Bse118I RCCGGY 1 cut(s) 393
Bse1I ACTGG 1 cut(s) 896
BseAI TCCGGA 1 cut(s) 197
BseBI CCWGG 1 cut(s) 951
BseDI CCNNGG 4 cut(s) 15, 386, 577, 950
BseGI GGATG 3 cut(s) 116, 161, 194
BseLI CCNNNNNNNGG 7 cut(s) 175, 260, 367, 387, 393, 453, 614
BseMII CTCAG 2 cut(s) 898, 1074
BseNI ACTGG 1 cut(s) 896
BseRI GAGGAG 4 cut(s) 71, 350, 353, 630
BseSI GKGCMC 1 cut(s) 332
BseXI GCAGC 2 cut(s) 359, 678
BseYI CCCAGC 1 cut(s) 597
Bsh1236I CGCG 1 cut(s) 663
BshFI GGCC 8 cut(s) 407, 621, 650, 681, 704, 771, 949, 1080
BshNI GGYRCC 2 cut(s) 390, 396
BsiHKAI GWGCWC 1 cut(s) 345
BsiSI CCGG 7 cut(s) 176, 198, 284, 388, 394, 404, 416
BslI CCNNNNNNNGG 7 cut(s) 175, 260, 367, 387, 393, 453, 614
BsmAI GTCTC 1 cut(s) 597
BsmI GAATGC 1 cut(s) 669
BsnI GGCC 8 cut(s) 407, 621, 650, 681, 704, 771, 949, 1080
Bsp1286I GDGCHC 3 cut(s) 332, 345, 381
Bsp13I TCCGGA 1 cut(s) 197
Bsp1407I TGTACA 1 cut(s) 933
Bsp143I GATC 5 cut(s) 427, 675, 991, 1020, 1072
Bsp19I CCATGG 1 cut(s) 15
BspACI CCGC 4 cut(s) 400, 663, 693, 696
BspANI GGCC 8 cut(s) 407, 621, 650, 681, 704, 771, 949, 1080
BspCNI CTCAG 2 cut(s) 897, 1075
BspEI TCCGGA 1 cut(s) 197
BspFNI CGCG 1 cut(s) 663
BspLI GGNNCC 2 cut(s) 392, 398
BspPI GGATC 2 cut(s) 422, 683
BspQI GCTCTTC 1 cut(s) 284
BspT107I GGYRCC 2 cut(s) 390, 396
BsrFI RCCGGY 1 cut(s) 393
BsrGI TGTACA 1 cut(s) 933
BsrI ACTGG 1 cut(s) 896
BssAI RCCGGY 1 cut(s) 393
BssECI CCNNGG 4 cut(s) 15, 386, 577, 950
BssMI GATC 5 cut(s) 427, 675, 991, 1020, 1072
BssNI GRCGYC 2 cut(s) 391, 752
BssSI CACGAG 1 cut(s) 1135
BssT1I CCWWGG 1 cut(s) 15
Bst2BI CACGAG 1 cut(s) 1135
Bst2UI CCWGG 1 cut(s) 951
Bst6I CTCTTC 2 cut(s) 284, 478
BstACI GRCGYC 2 cut(s) 391, 752
BstAPI GCANNNNNTGC 2 cut(s) 270, 564
BstAUI TGTACA 1 cut(s) 933
BstC8I GCNNGC 5 cut(s) 262, 271, 275, 377, 604
BstDEI CTNAG 5 cut(s) 346, 884, 944, 1083, 1088
BstDSI CCRYGG 1 cut(s) 15
BstENI CCTNNNNNAGG 1 cut(s) 365
BstF5I GGATG 3 cut(s) 116, 161, 194
BstFNI CGCG 1 cut(s) 663
BstH2I RGCGCY 1 cut(s) 394
BstHHI GCGC 1 cut(s) 393
BstKTI GATC 5 cut(s) 430, 678, 994, 1023, 1075
BstMAI GTCTC 1 cut(s) 597
BstMBI GATC 5 cut(s) 427, 675, 991, 1020, 1072
BstMWI GCNNNNNNNGC 6 cut(s) 266, 270, 399, 564, 603, 710
BstNI CCWGG 1 cut(s) 951
BstNSI RCATGY 2 cut(s) 273, 307
BstSCI CCNGG 4 cut(s) 282, 386, 402, 949
BstSFI CTRYAG 1 cut(s) 493
BstSLI GKGCMC 1 cut(s) 332
BstUI CGCG 1 cut(s) 663
BstV1I GCAGC 2 cut(s) 359, 678
BsuI GTATCC 1 cut(s) 212
BsuRI GGCC 8 cut(s) 407, 621, 650, 681, 704, 771, 949, 1080
BtgI CCRYGG 1 cut(s) 15
BtsCI GGATG 3 cut(s) 116, 161, 194
BtsIMutI CAGTG 2 cut(s) 423, 483
Cac8I GCNNGC 5 cut(s) 262, 271, 275, 377, 604
CaiI CAGNNNCTG 1 cut(s) 891
CfoI GCGC 1 cut(s) 393
Cfr10I RCCGGY 1 cut(s) 393
Cfr13I GGNCC 2 cut(s) 770, 1079
CseI GACGC 1 cut(s) 780
Csp6I GTAC 4 cut(s) 421, 866, 934, 1099
CviAII CATG 4 cut(s) 16, 270, 304, 874
CviQI GTAC 4 cut(s) 421, 866, 934, 1099
DdeI CTNAG 5 cut(s) 346, 884, 944, 1083, 1088
DinI GGCGCC 1 cut(s) 392
DpnI GATC 5 cut(s) 429, 677, 993, 1022, 1074
DpnII GATC 5 cut(s) 427, 675, 991, 1020, 1072
DrdI GACNNNNNNGTC 1 cut(s) 463
DseDI GACNNNNNNGTC 1 cut(s) 463
EaeI YGGCCR 2 cut(s) 648, 702
Eam1104I CTCTTC 2 cut(s) 284, 478
EarI CTCTTC 2 cut(s) 284, 478
Ecl136II GAGCTC 1 cut(s) 343
Eco130I CCWWGG 1 cut(s) 15
Eco147I AGGCCT 2 cut(s) 621, 949
Eco24I GRGCYC 2 cut(s) 345, 381
Eco53kI GAGCTC 1 cut(s) 343
Eco57I CTGAAG 1 cut(s) 459
EcoICRI GAGCTC 1 cut(s) 343
EcoNI CCTNNNNNAGG 1 cut(s) 365
EcoO109I RGGNCCY 1 cut(s) 1079
EcoRII CCWGG 1 cut(s) 949
EcoT14I CCWWGG 1 cut(s) 15
EcoT38I GRGCYC 2 cut(s) 345, 381
EgeI GGCGCC 1 cut(s) 392
EheI GGCGCC 1 cut(s) 392
ErhI CCWWGG 1 cut(s) 15
FaeI CATG 4 cut(s) 19, 273, 307, 877
FatI CATG 4 cut(s) 15, 269, 303, 873
FauI CCCGC 1 cut(s) 689
FbaI TGATCA 1 cut(s) 991
Fnu4HI GCNGC 5 cut(s) 373, 400, 664, 667, 694
FokI GGATG 3 cut(s) 103, 148, 181
FriOI GRGCYC 2 cut(s) 345, 381
Fsp4HI GCNGC 5 cut(s) 373, 400, 664, 667, 694
GlaI GCGC 1 cut(s) 392
GluI GCNGC 5 cut(s) 373, 400, 664, 667, 694
GsaI CCCAGC 1 cut(s) 601
GsuI CTGGAG 1 cut(s) 240
HaeII RGCGCY 1 cut(s) 394
HaeIII GGCC 8 cut(s) 407, 621, 650, 681, 704, 771, 949, 1080
HapII CCGG 7 cut(s) 176, 198, 284, 388, 394, 404, 416
HgaI GACGC 1 cut(s) 780
HhaI GCGC 1 cut(s) 393
Hin1I GRCGYC 2 cut(s) 391, 752
Hin1II CATG 4 cut(s) 19, 273, 307, 877
Hin6I GCGC 1 cut(s) 391
HinP1I GCGC 1 cut(s) 391
HincII GTYRAC 1 cut(s) 523
HindII GTYRAC 1 cut(s) 523
HindIII AAGCTT 1 cut(s) 1067
HinfI GANTC 6 cut(s) 20, 236, 470, 805, 895, 1035
HpaII CCGG 7 cut(s) 176, 198, 284, 388, 394, 404, 416
HphI GGTGA 5 cut(s) 40, 91, 541, 1001, 1015
Hpy166II GTNNAC 5 cut(s) 423, 486, 523, 868, 934
Hpy188I TCNGA 7 cut(s) 166, 218, 241, 469, 578, 675, 900
Hpy188III TCNNGA 5 cut(s) 85, 148, 198, 795, 1024
Hpy8I GTNNAC 5 cut(s) 423, 486, 523, 868, 934
Hpy99I CGWCG 8 cut(s) 300, 341, 689, 754, 757, 796, 904, 907
HpyAV CCTTC 3 cut(s) 80, 86, 199
HpyCH4IV ACGT 5 cut(s) 687, 707, 752, 879, 905
HpyCH4V TGCA 4 cut(s) 273, 501, 558, 877
HpyF10VI GCNNNNNNNGC 6 cut(s) 266, 270, 399, 564, 603, 710
HpyF3I CTNAG 5 cut(s) 346, 884, 944, 1083, 1088
HpySE526I ACGT 5 cut(s) 687, 707, 752, 879, 905
Hsp92I GRCGYC 2 cut(s) 391, 752
Hsp92II CATG 4 cut(s) 19, 273, 307, 877
HspAI GCGC 1 cut(s) 391
KasI GGCGCC 1 cut(s) 390
Kpn2I TCCGGA 1 cut(s) 197
Ksp22I TGATCA 1 cut(s) 991
Kzo9I GATC 5 cut(s) 427, 675, 991, 1020, 1072
LguI GCTCTTC 1 cut(s) 284
LmnI GCTCC 1 cut(s) 363
Lsp1109I GCAGC 2 cut(s) 359, 678
LweI GCATC 3 cut(s) 170, 203, 488
MaeII ACGT 5 cut(s) 687, 707, 752, 879, 905
MaeIII GTNAC 3 cut(s) 745, 887, 1003
MalI GATC 5 cut(s) 429, 677, 993, 1022, 1074
MboI GATC 5 cut(s) 427, 675, 991, 1020, 1072
MboII GAAGA 3 cut(s) 271, 465, 1141
MfeI CAATTG 1 cut(s) 995
MhlI GDGCHC 3 cut(s) 332, 345, 381
MlsI TGGCCA 2 cut(s) 650, 704
MluCI AATT 2 cut(s) 91, 995
MluNI TGGCCA 2 cut(s) 650, 704
Mly113I GGCGCC 1 cut(s) 391
MlyI GAGTC 1 cut(s) 464
MmeI TCCRAC 5 cut(s) 189, 264, 492, 923, 1095
Mox20I TGGCCA 2 cut(s) 650, 704
MroI TCCGGA 1 cut(s) 197
MroXI GAANNNNTTC 1 cut(s) 545
MscI TGGCCA 2 cut(s) 650, 704
Msp20I TGGCCA 2 cut(s) 650, 704
MspA1I CMGCKG 1 cut(s) 260
MspI CCGG 7 cut(s) 176, 198, 284, 388, 394, 404, 416
MspR9I CCNGG 4 cut(s) 284, 388, 404, 951
MunI CAATTG 1 cut(s) 995
Mva1269I GAATGC 1 cut(s) 669
MvaI CCWGG 1 cut(s) 951
MvnI CGCG 1 cut(s) 663
MwoI GCNNNNNNNGC 6 cut(s) 266, 270, 399, 564, 603, 710
NarI GGCGCC 1 cut(s) 391
NciI CCSGG 3 cut(s) 284, 388, 404
NcoI CCATGG 1 cut(s) 15
NdeII GATC 5 cut(s) 427, 675, 991, 1020, 1072
NlaIII CATG 4 cut(s) 19, 273, 307, 877
NlaIV GGNNCC 2 cut(s) 392, 398
NmuCI GTSAC 1 cut(s) 1003
NspI RCATGY 2 cut(s) 273, 307
PaeI GCATGC 1 cut(s) 273
PceI AGGCCT 2 cut(s) 621, 949
PciI ACATGT 1 cut(s) 303
PciSI GCTCTTC 1 cut(s) 284
PcsI WCGNNNNNNNCGW 1 cut(s) 684
PctI GAATGC 1 cut(s) 669
PdmI GAANNNNTTC 1 cut(s) 545
PfeI GAWTC 5 cut(s) 20, 236, 805, 895, 1035
PflMI CCANNNNNTGG 1 cut(s) 453
PfoI TCCNGGA 1 cut(s) 282
PkrI GCNGC 5 cut(s) 374, 401, 665, 668, 695
PleI GAGTC 1 cut(s) 464
PluTI GGCGCC 1 cut(s) 394
PpsI GAGTC 1 cut(s) 464
PscI ACATGT 1 cut(s) 303
PsiI TTATAA 1 cut(s) 1011
Psp124BI GAGCTC 1 cut(s) 345
Psp6I CCWGG 1 cut(s) 949
PspFI CCCAGC 1 cut(s) 597
PspGI CCWGG 1 cut(s) 949
PspN4I GGNNCC 2 cut(s) 392, 398
PspPI GGNCC 2 cut(s) 770, 1079
PstNI CAGNNNCTG 1 cut(s) 891
PvuII CAGCTG 1 cut(s) 260
RsaI GTAC 4 cut(s) 422, 867, 935, 1100
RsaNI GTAC 4 cut(s) 421, 866, 934, 1099
SacI GAGCTC 1 cut(s) 345
SapI GCTCTTC 1 cut(s) 284
SatI GCNGC 5 cut(s) 373, 400, 664, 667, 694
Sau3AI GATC 5 cut(s) 427, 675, 991, 1020, 1072
Sau96I GGNCC 2 cut(s) 770, 1079
SchI GAGTC 1 cut(s) 464
ScrFI CCNGG 4 cut(s) 284, 388, 404, 951
SduI GDGCHC 3 cut(s) 332, 345, 381
SfaNI GCATC 3 cut(s) 170, 203, 488
SfcI CTRYAG 1 cut(s) 493
SfoI GGCGCC 1 cut(s) 392
SgrAI CRCCGGYG 1 cut(s) 393
SmlI CTYRAG 1 cut(s) 365
SmoI CTYRAG 1 cut(s) 365
SphI GCATGC 1 cut(s) 273
Sse9I AATT 2 cut(s) 91, 995
SseBI AGGCCT 2 cut(s) 621, 949
SsiI CCGC 4 cut(s) 400, 663, 693, 696
SspDI GGCGCC 1 cut(s) 390
SstI GAGCTC 1 cut(s) 345
StuI AGGCCT 2 cut(s) 621, 949
StyD4I CCNGG 4 cut(s) 282, 386, 402, 949
StyI CCWWGG 1 cut(s) 15
TaiI ACGT 5 cut(s) 690, 710, 755, 882, 908
TaqI TCGA 9 cut(s) 53, 149, 570, 684, 755, 794, 969, 1023, 1075
TasI AATT 2 cut(s) 91, 995
TatI WGTACW 2 cut(s) 420, 933
TauI GCSGC 3 cut(s) 402, 666, 696
TfiI GAWTC 5 cut(s) 20, 236, 805, 895, 1035
TscAI CASTG 2 cut(s) 430, 483
TseFI GTSAC 1 cut(s) 1003
TseI GCWGC 2 cut(s) 372, 666
Tsp45I GTSAC 1 cut(s) 1003
TspDTI ATGAA 5 cut(s) 17, 85, 773, 775, 902
TspGWI ACGGA 1 cut(s) 828
TspRI CASTG 2 cut(s) 430, 483
Van91I CCANNNNNTGG 1 cut(s) 453
XagI CCTNNNNNAGG 1 cut(s) 365
XapI RAATTY 1 cut(s) 91
XceI RCATGY 2 cut(s) 273, 307
XmnI GAANNNNTTC 1 cut(s) 545
ZraI GACGTC 1 cut(s) 753
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.