Rmu_sc0003156.1_g000020

Putative S-adenosyl-L-methionine-dependent methyltransferase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_multiflora
Rmu_sc0003156.1
OG0017593
Physical Location & Seq
Forward (+)
121702 .. 122862
1161 bp
Loading structure...
UTR
Exon/CDS
Intron
Rmu_sc0003156.1_g000020.1.cds

Sequence Viewer

Length: 1161 bp
atgaagcaaaaatccacggattcccctcatgataaattcaagtcttcttcctcgaataacctcttcttccttctccttctcctcttaacaaatctcatcaccttgttcatttcctcatcccttttctcctgttcctacaagcccctctccaccaccgtcgaggaaaccgccaccatagcatccgacctcccgcccgaattccttgccttcacatccggacaagctctcccgttcggattcaacagcaacttcgattccgacaccatttaccctccagcaggcaaagcatgcacactcttccccgacgaactccgtcgctacatgtcatacaaagtcaacggttcgtgtcctgacgacgagctcctagcccaaaagctcctcctcaagggctgcgagcccttacctcggcgccggtgccgccccgcctctcaaccggagtacactgaaccctaccctctccccatcagcctatggaccacgccgtccgactcttcagtcgtgtggacagcgtacacatgcaagaactacaactgccttgtcaacagaaaacgaaacgagaaaggcttctccgactgcaaagactgcttcgacctccaaggaaacgagaagtctcgctgggcaaggtctaaaggagggggcctcgatttcagcatagacgaagtcttggccaccaagccacacggcagcatacgaatcggcctcgacgtcggcggcggggtggccacgttcgccgtgagaatgagagagaggaacataacgatcgtaacgacgtcgatgaacttgaacggccctttcaacaacttcatagcgtcgagaggtgtggttcctctctacataagcatttcgcagaggctgccgttctacgacaacactttggacatcgtacactccatgcacgttctcagtaactggattccgacgacattgcttcattttgccttctttgatgtctacagagtgcttaggcctggggggttgttctggctcgaccattttttctgtgtgggtgagcagttggaggagatatatggaccaatgatcgagagtattggattcaacaagttgaagtgggttgttgggaggaagcttgatcgaggccctgagcgtagggagatgtacctttcggctttgttggagaagccattgaagaactcgtggtga
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Methyltransf_11 Methyltransf_29

Protein Analysis

386

Amino Acids

43.66

Weight (kDa)

8.44

Isoelectric Point (pI)

48.94

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0017593)

Species Orthologous Gene IDs
fragaria_vesca FvH4_1g29960
malus_domestica MD17G1262900.v1.1
prunus_persica Prupe.3G164600_v2.0.a1
rosa_chinensis RchiOBHm_Chr3g0492371
rosa_laevigata RLG00000022779
rosa_multiflora Rmu_sc0003156.1_g000020
rosa_roxburghii Rroxscaffold_6G00392380
rosa_samantha Rh3AG303400 Rh3BG339600 Rh3CG336600 Rh3DG338700
rosa_wichuraiana Rw3G026770

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 2 cut(s) 481, 494
AatII GACGTC 2 cut(s) 708, 773
AccB1I GGYRCC 2 cut(s) 408, 414
AccI GTMKAC 1 cut(s) 951
AccIII TCCGGA 1 cut(s) 215
AciI CCGC 6 cut(s) 168, 191, 418, 423, 711, 714
AcoI YGGCCR 2 cut(s) 666, 720
AcsI RAATTY 2 cut(s) 35, 197
AcuI CTGAAG 1 cut(s) 477
AcyI GRCGYC 3 cut(s) 409, 705, 770
AfaI GTAC 4 cut(s) 440, 512, 885, 1118
AfiI CCNNNNNNNGG 3 cut(s) 278, 385, 405
AflIII ACRYGT 1 cut(s) 321
AgsI TTSAA 7 cut(s) 40, 241, 784, 796, 1057, 1066, 1147
AjnI CCWGG 1 cut(s) 967
AluBI AGCT 4 cut(s) 224, 361, 376, 1087
AluI AGCT 4 cut(s) 224, 361, 376, 1087
Alw21I GWGCWC 1 cut(s) 363
Alw26I GTCTC 1 cut(s) 615
AlwNI CAGNNNCTG 2 cut(s) 853, 909
Aor13HI TCCGGA 1 cut(s) 215
AoxI GGCC 7 cut(s) 637, 666, 697, 720, 787, 965, 1096
ApeKI GCWGC 3 cut(s) 390, 684, 853
ApoI RAATTY 2 cut(s) 35, 197
Asp700I GAANNNNTTC 1 cut(s) 563
AspLEI GCGC 1 cut(s) 411
AspS9I GGNCC 5 cut(s) 474, 637, 788, 1031, 1097
AsuHPI GGTGA 2 cut(s) 91, 1019
AvaII GGWCC 2 cut(s) 474, 1031
BalI TGGCCA 2 cut(s) 668, 722
BanI GGYRCC 2 cut(s) 408, 414
BanII GRGCYC 2 cut(s) 363, 399
BauI CACGAG 1 cut(s) 1153
BbsI GAAGAC 1 cut(s) 36
Bbv12I GWGCWC 1 cut(s) 363
BbvI GCAGC 3 cut(s) 377, 696, 840
BccI CCATC 1 cut(s) 470
BceAI ACGGC 5 cut(s) 466, 697, 716, 802, 841
BcgI CGANNNNNNTGC 6 cut(s) 185, 219, 676, 710, 907, 941
BciT130I CCWGG 1 cut(s) 969
BcoDI GTCTC 1 cut(s) 615
BfaI CTAG 1 cut(s) 365
BfmI CTRYAG 1 cut(s) 952
BfoI RGCGCY 1 cut(s) 412
BisI GCNGC 5 cut(s) 391, 418, 685, 712, 854
BlsI GCNGC 5 cut(s) 392, 419, 686, 713, 855
Bme1390I CCNGG 1 cut(s) 969
Bme18I GGWCC 2 cut(s) 474, 1031
BmgT120I GGNCC 5 cut(s) 474, 637, 788, 1031, 1097
BmiI GGNNCC 4 cut(s) 410, 416, 638, 825
BmrFI CCNGG 1 cut(s) 969
BmsI GCATC 1 cut(s) 188
BpiI GAAGAC 1 cut(s) 36
BplI GAGNNNNNCTC 2 cut(s) 624, 656
BpmI CTGGAG 1 cut(s) 258
Bpu10I CCTNAGC 2 cut(s) 962, 1101
BpuEI CTTGAG 1 cut(s) 368
BsaHI GRCGYC 3 cut(s) 409, 705, 770
BsaJI CCNNGG 4 cut(s) 15, 404, 595, 968
BsaWI WCCGGW 2 cut(s) 215, 433
BsaXI ACNNNNNCTCC 2 cut(s) 210, 240
Bsc4I CCNNNNNNNGG 3 cut(s) 278, 385, 405
Bse118I RCCGGY 1 cut(s) 411
Bse1I ACTGG 1 cut(s) 914
Bse3DI GCAATG 1 cut(s) 923
BseAI TCCGGA 1 cut(s) 215
BseBI CCWGG 1 cut(s) 969
BseDI CCNNGG 4 cut(s) 15, 404, 595, 968
BseGI GGATG 3 cut(s) 116, 179, 212
BseLI CCNNNNNNNGG 3 cut(s) 278, 385, 405
BseMI GCAATG 1 cut(s) 923
BseMII CTCAG 2 cut(s) 916, 1092
BseNI ACTGG 1 cut(s) 914
BseRI GAGGAG 4 cut(s) 71, 368, 371, 1034
BseXI GCAGC 3 cut(s) 377, 696, 840
BseYI CCCAGC 1 cut(s) 615
Bsh1285I CGRYCG 1 cut(s) 762
BshFI GGCC 7 cut(s) 639, 668, 699, 722, 789, 967, 1098
BshNI GGYRCC 2 cut(s) 408, 414
BsiEI CGRYCG 1 cut(s) 762
BsiHKAI GWGCWC 1 cut(s) 363
BsiSI CCGG 3 cut(s) 216, 412, 434
BslI CCNNNNNNNGG 3 cut(s) 278, 385, 405
BsmAI GTCTC 1 cut(s) 615
BsnI GGCC 7 cut(s) 639, 668, 699, 722, 789, 967, 1098
Bsp1286I GDGCHC 2 cut(s) 363, 399
Bsp13I TCCGGA 1 cut(s) 215
Bsp143I GATC 3 cut(s) 759, 1038, 1090
BspACI CCGC 6 cut(s) 168, 191, 418, 423, 711, 714
BspANI GGCC 7 cut(s) 639, 668, 699, 722, 789, 967, 1098
BspCNI CTCAG 2 cut(s) 915, 1093
BspEI TCCGGA 1 cut(s) 215
BspHI TCATGA 1 cut(s) 28
BspLI GGNNCC 4 cut(s) 410, 416, 638, 825
BspT107I GGYRCC 2 cut(s) 408, 414
BsrDI GCAATG 1 cut(s) 923
BsrFI RCCGGY 1 cut(s) 411
BsrI ACTGG 1 cut(s) 914
BssAI RCCGGY 1 cut(s) 411
BssECI CCNNGG 4 cut(s) 15, 404, 595, 968
BssMI GATC 3 cut(s) 759, 1038, 1090
BssNI GRCGYC 3 cut(s) 409, 705, 770
BssSI CACGAG 1 cut(s) 1153
BssT1I CCWWGG 1 cut(s) 595
Bst2BI CACGAG 1 cut(s) 1153
Bst2UI CCWGG 1 cut(s) 969
Bst4CI ACNGT 2 cut(s) 157, 341
Bst6I CTCTTC 3 cut(s) 68, 302, 496
BstACI GRCGYC 3 cut(s) 409, 705, 770
BstAPI GCANNNNNTGC 3 cut(s) 288, 582, 853
BstC8I GCNNGC 3 cut(s) 280, 289, 395
BstDEI CTNAG 3 cut(s) 902, 962, 1101
BstDSI CCRYGG 1 cut(s) 15
BstENI CCTNNNNNAGG 2 cut(s) 276, 383
BstF5I GGATG 3 cut(s) 116, 179, 212
BstH2I RGCGCY 1 cut(s) 412
BstHHI GCGC 1 cut(s) 411
BstKTI GATC 3 cut(s) 762, 1041, 1093
BstMAI GTCTC 1 cut(s) 615
BstMBI GATC 3 cut(s) 759, 1038, 1090
BstMCI CGRYCG 1 cut(s) 762
BstMWI GCNNNNNNNGC 7 cut(s) 176, 284, 288, 417, 582, 728, 853
BstNI CCWGG 1 cut(s) 969
BstNSI RCATGY 3 cut(s) 291, 325, 519
BstSCI CCNGG 1 cut(s) 967
BstSFI CTRYAG 1 cut(s) 952
BstV1I GCAGC 3 cut(s) 377, 696, 840
BstV2I GAAGAC 1 cut(s) 36
BsuRI GGCC 7 cut(s) 639, 668, 699, 722, 789, 967, 1098
BtgI CCRYGG 1 cut(s) 15
BtsCI GGATG 3 cut(s) 116, 179, 212
BtsIMutI CAGTG 1 cut(s) 441
Cac8I GCNNGC 3 cut(s) 280, 289, 395
CaiI CAGNNNCTG 2 cut(s) 853, 909
CciI TCATGA 1 cut(s) 28
CfoI GCGC 1 cut(s) 411
Cfr10I RCCGGY 1 cut(s) 411
Cfr13I GGNCC 5 cut(s) 474, 637, 788, 1031, 1097
CseI GACGC 1 cut(s) 798
Csp6I GTAC 4 cut(s) 439, 511, 884, 1117
CviAII CATG 5 cut(s) 29, 288, 322, 516, 892
CviQI GTAC 4 cut(s) 439, 511, 884, 1117
DdeI CTNAG 3 cut(s) 902, 962, 1101
DinI GGCGCC 1 cut(s) 410
DpnI GATC 3 cut(s) 761, 1040, 1092
DpnII GATC 3 cut(s) 759, 1038, 1090
DrdI GACNNNNNNGTC 2 cut(s) 481, 494
DseDI GACNNNNNNGTC 2 cut(s) 481, 494
EaeI YGGCCR 2 cut(s) 666, 720
Eam1104I CTCTTC 3 cut(s) 68, 302, 496
EarI CTCTTC 3 cut(s) 68, 302, 496
Ecl136II GAGCTC 1 cut(s) 361
Eco130I CCWWGG 1 cut(s) 595
Eco147I AGGCCT 1 cut(s) 967
Eco24I GRGCYC 2 cut(s) 363, 399
Eco47I GGWCC 2 cut(s) 474, 1031
Eco53kI GAGCTC 1 cut(s) 361
Eco57I CTGAAG 1 cut(s) 477
EcoICRI GAGCTC 1 cut(s) 361
EcoNI CCTNNNNNAGG 2 cut(s) 276, 383
EcoO109I RGGNCCY 2 cut(s) 637, 1097
EcoRI GAATTC 1 cut(s) 197
EcoRII CCWGG 1 cut(s) 967
EcoT14I CCWWGG 1 cut(s) 595
EcoT38I GRGCYC 2 cut(s) 363, 399
EgeI GGCGCC 1 cut(s) 410
EheI GGCGCC 1 cut(s) 410
ErhI CCWWGG 1 cut(s) 595
FaeI CATG 5 cut(s) 32, 291, 325, 519, 895
FatI CATG 5 cut(s) 28, 287, 321, 515, 891
FauI CCCGC 3 cut(s) 198, 430, 707
FblI GTMKAC 1 cut(s) 951
Fnu4HI GCNGC 5 cut(s) 391, 418, 685, 712, 854
FokI GGATG 3 cut(s) 103, 166, 199
FriOI GRGCYC 2 cut(s) 363, 399
Fsp4HI GCNGC 5 cut(s) 391, 418, 685, 712, 854
FspBI CTAG 1 cut(s) 365
GlaI GCGC 1 cut(s) 410
GluI GCNGC 5 cut(s) 391, 418, 685, 712, 854
GsaI CCCAGC 1 cut(s) 619
GsuI CTGGAG 1 cut(s) 258
HaeII RGCGCY 1 cut(s) 412
HaeIII GGCC 7 cut(s) 639, 668, 699, 722, 789, 967, 1098
HapII CCGG 3 cut(s) 216, 412, 434
HgaI GACGC 1 cut(s) 798
HhaI GCGC 1 cut(s) 411
Hin1I GRCGYC 3 cut(s) 409, 705, 770
Hin1II CATG 5 cut(s) 32, 291, 325, 519, 895
Hin6I GCGC 1 cut(s) 409
HinP1I GCGC 1 cut(s) 409
HincII GTYRAC 2 cut(s) 337, 541
HindII GTYRAC 2 cut(s) 337, 541
HindIII AAGCTT 1 cut(s) 1085
HinfI GANTC 7 cut(s) 20, 237, 254, 488, 693, 913, 1053
HpaII CCGG 3 cut(s) 216, 412, 434
HphI GGTGA 2 cut(s) 91, 1019
Hpy166II GTNNAC 7 cut(s) 337, 441, 504, 513, 541, 886, 952
Hpy188I TCNGA 6 cut(s) 184, 236, 259, 487, 571, 918
Hpy188III TCNNGA 5 cut(s) 29, 216, 350, 813, 1042
Hpy8I GTNNAC 7 cut(s) 337, 441, 504, 513, 541, 886, 952
HpyAV CCTTC 4 cut(s) 80, 86, 217, 949
HpyCH4III ACNGT 2 cut(s) 157, 341
HpyCH4IV ACGT 4 cut(s) 705, 725, 770, 897
HpyCH4V TGCA 4 cut(s) 291, 519, 576, 895
HpyF10VI GCNNNNNNNGC 7 cut(s) 176, 284, 288, 417, 582, 728, 853
HpyF3I CTNAG 3 cut(s) 902, 962, 1101
HpySE526I ACGT 4 cut(s) 705, 725, 770, 897
Hsp92I GRCGYC 3 cut(s) 409, 705, 770
Hsp92II CATG 5 cut(s) 32, 291, 325, 519, 895
HspAI GCGC 1 cut(s) 409
KasI GGCGCC 1 cut(s) 408
Kpn2I TCCGGA 1 cut(s) 215
Kzo9I GATC 3 cut(s) 759, 1038, 1090
LmnI GCTCC 2 cut(s) 366, 381
Lsp1109I GCAGC 3 cut(s) 377, 696, 840
LweI GCATC 1 cut(s) 188
MaeI CTAG 1 cut(s) 365
MaeII ACGT 4 cut(s) 705, 725, 770, 897
MaeIII GTNAC 2 cut(s) 763, 905
MalI GATC 3 cut(s) 761, 1040, 1092
MboI GATC 3 cut(s) 759, 1038, 1090
MboII GAAGA 7 cut(s) 36, 39, 55, 58, 289, 483, 1159
MhlI GDGCHC 2 cut(s) 363, 399
MlsI TGGCCA 2 cut(s) 668, 722
MluCI AATT 2 cut(s) 35, 197
MluNI TGGCCA 2 cut(s) 668, 722
Mly113I GGCGCC 1 cut(s) 409
MlyI GAGTC 1 cut(s) 482
MmeI TCCRAC 7 cut(s) 207, 282, 510, 594, 941, 996, 1113
Mox20I TGGCCA 2 cut(s) 668, 722
MroI TCCGGA 1 cut(s) 215
MroXI GAANNNNTTC 1 cut(s) 563
MscI TGGCCA 2 cut(s) 668, 722
MseI TTAA 1 cut(s) 86
Msp20I TGGCCA 2 cut(s) 668, 722
MspI CCGG 3 cut(s) 216, 412, 434
MspR9I CCNGG 1 cut(s) 969
MvaI CCWGG 1 cut(s) 969
MwoI GCNNNNNNNGC 7 cut(s) 176, 284, 288, 417, 582, 728, 853
NarI GGCGCC 1 cut(s) 409
NdeII GATC 3 cut(s) 759, 1038, 1090
NlaIII CATG 5 cut(s) 32, 291, 325, 519, 895
NlaIV GGNNCC 4 cut(s) 410, 416, 638, 825
NmeAIII GCCGAG 1 cut(s) 385
NspI RCATGY 3 cut(s) 291, 325, 519
PaeI GCATGC 1 cut(s) 291
PagI TCATGA 1 cut(s) 28
PceI AGGCCT 1 cut(s) 967
PciI ACATGT 1 cut(s) 321
PcsI WCGNNNNNNNCGW 2 cut(s) 702, 764
PdmI GAANNNNTTC 1 cut(s) 563
PfeI GAWTC 6 cut(s) 20, 237, 254, 693, 913, 1053
PflFI GACNNNGTC 1 cut(s) 659
PkrI GCNGC 5 cut(s) 392, 419, 686, 713, 855
Ple19I CGATCG 1 cut(s) 762
PleI GAGTC 1 cut(s) 482
PluTI GGCGCC 1 cut(s) 412
PpsI GAGTC 1 cut(s) 482
PscI ACATGT 1 cut(s) 321
Psp124BI GAGCTC 1 cut(s) 363
Psp6I CCWGG 1 cut(s) 967
PspFI CCCAGC 1 cut(s) 615
PspGI CCWGG 1 cut(s) 967
PspN4I GGNNCC 4 cut(s) 410, 416, 638, 825
PspPI GGNCC 5 cut(s) 474, 637, 788, 1031, 1097
PstNI CAGNNNCTG 2 cut(s) 853, 909
PsyI GACNNNGTC 1 cut(s) 659
PvuI CGATCG 1 cut(s) 762
RsaI GTAC 4 cut(s) 440, 512, 885, 1118
RsaNI GTAC 4 cut(s) 439, 511, 884, 1117
SacI GAGCTC 1 cut(s) 363
SaqAI TTAA 1 cut(s) 86
SatI GCNGC 5 cut(s) 391, 418, 685, 712, 854
Sau3AI GATC 3 cut(s) 759, 1038, 1090
Sau96I GGNCC 5 cut(s) 474, 637, 788, 1031, 1097
SchI GAGTC 1 cut(s) 482
ScrFI CCNGG 1 cut(s) 969
SduI GDGCHC 2 cut(s) 363, 399
SfaNI GCATC 1 cut(s) 188
SfcI CTRYAG 1 cut(s) 952
SfoI GGCGCC 1 cut(s) 410
SgrAI CRCCGGYG 1 cut(s) 411
SinI GGWCC 2 cut(s) 474, 1031
SmlI CTYRAG 1 cut(s) 383
SmoI CTYRAG 1 cut(s) 383
SphI GCATGC 1 cut(s) 291
Sse9I AATT 2 cut(s) 35, 197
SseBI AGGCCT 1 cut(s) 967
SsiI CCGC 6 cut(s) 168, 191, 418, 423, 711, 714
SspDI GGCGCC 1 cut(s) 408
SspMI CTAG 1 cut(s) 365
SstI GAGCTC 1 cut(s) 363
StuI AGGCCT 1 cut(s) 967
StyD4I CCNGG 1 cut(s) 967
StyI CCWWGG 1 cut(s) 595
TaaI ACNGT 2 cut(s) 157, 341
TaiI ACGT 4 cut(s) 708, 728, 773, 900
TasI AATT 2 cut(s) 35, 197
TatI WGTACW 1 cut(s) 438
TauI GCSGC 2 cut(s) 420, 714
TfiI GAWTC 6 cut(s) 20, 237, 254, 693, 913, 1053
Tru1I TTAA 1 cut(s) 86
Tru9I TTAA 1 cut(s) 86
TscAI CASTG 1 cut(s) 448
TseI GCWGC 3 cut(s) 390, 684, 853
TspDTI ATGAA 5 cut(s) 17, 97, 791, 793, 920
TspGWI ACGGA 2 cut(s) 32, 302
TspRI CASTG 1 cut(s) 448
Tth111I GACNNNGTC 1 cut(s) 659
VpaK11BI GGWCC 2 cut(s) 474, 1031
XagI CCTNNNNNAGG 2 cut(s) 276, 383
XapI RAATTY 2 cut(s) 35, 197
XceI RCATGY 3 cut(s) 291, 325, 519
XmiI GTMKAC 1 cut(s) 951
XmnI GAANNNNTTC 1 cut(s) 563
XspI CTAG 1 cut(s) 365
ZraI GACGTC 2 cut(s) 706, 771
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.