MD17G1262900.v1.1

Putative S-adenosyl-L-methionine-dependent methyltransferase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr17
OG0017593
Physical Location & Seq
Forward (+)
32331139 .. 32332560
1422 bp
Loading structure...
UTR
Exon/CDS
Intron
MD17G1262900.v1.1.491

Sequence Viewer

Length: 1329 bp
ATGGCCTCGGATTCCCCACTTAAATCCAAGCCTCCATACTCCTCAAATATCTTGTTCCTTTTCCTTCTCCTTTCAACAAATCTCATCACCTTGTTCCTCTCCTCATCATTTTCGTTTTGCGCCCTCAAACCTTCTACAACAACCACCGCAGCAGCCACCGCGGATCCCGCCACTCCAACGACCGGCTCCAACCTCCCTTATGCCTCTACGGAAGACACATCTAACCTTCCAGCTGAATTTCTTGCCTTTGCGTCCGGACAACTCCTCCCATTTGGATACAATGCAAACTTTGACTCCAACACCATATATCCTCCAGTTGGACAAGCATGCACACTCTTCCCTGATCTCCTCCGCCGCTTCATGTCCTACAAGGTAAATGGATCGTGCCCGGATGACGAGTTTTTAGCCCAAAGGCTCCTCCTCAAGGGCTGCGAACCCTTACCACGCCGACGCTGCCGCCCTGCCTCAAACCCTAACTACGTGGAACCCTACCCTCTTCCCAAAAGCTACTGGTCCACACCGTCCGACAACTCAGTCGTATGGACAGCCTACAAATGCAAAAACTACGACTGCCTCATCAACCGAAAGAAAAACCACAAGGGCTTCGACGACTGTATAGACTGCTTTGACCTTCAAGGAAATGAGAAGAATCGGTGGGCAAAAGGCAAAGGAGGAGGGCTTGATTTCGGCGTGGATGAGGTCTTGGGGTTCAAGAAACCCCGAACGATACGAATAGGGCTTGACATCGGCGGTGGAGTGGCTACATTCGCTGTGAGAATGAAGGAGAGGAACATAACAATTTTGACAACTTCAATGAACTTGAATGGTCCTTTTAACAGCTTCATTGCTTCAAGAGGGGTCGTGCCTTTGTACCTAAGCATTTCGCAGAGGCTGCCGTTTTTCGATAATACGCTCGACATTGTGCATTCGATGCACGTCTTGAGTAACTGGATTCCGACGACGTTGCTTCATTTCGCTATGTTTGATATCTACAGGGTGCTTAGGCCTGGCGGGTTGTTTTGGCTTGACCATTTTTTCTGCGTAGGTGAGCAGTTGGAGGAGGTCTATGGACCTCTCATTGAGAGCATTGGGTACAAGAAGTTGAAGTGGGTTGTTGGGAAGAAGCTTGATCGTGGACCGGAGCGTAGGGAGATGTACCTCTCGGCGTTGCTGGAGAAGCCATTGAAGAACTCTTGTTACGTAGAAGAATATTTGAAAGAGAAGGAGATATGGAAATGGGCCGCGTGTATGCTGTCCCCAAAATACATGTCTCCTTCATGTCTTCTTGTTAATTTTGTGACTCCTATCTTGAAATTTAAATACTTTTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Methyltransf_11 Methyltransf_29

Protein Analysis

443

Amino Acids

49.89

Weight (kDa)

8.88

Isoelectric Point (pI)

46.87

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Methyltransf_29 PF03141 164 - 343 3.6e-09 Putative S-adenosyl-L-methionine-dependent methyltransferase
Methyltransf_11 PF08241 247 - 341 2.1e-06 Methyltransferase domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0017593)

Species Orthologous Gene IDs
fragaria_vesca FvH4_1g29960
malus_domestica MD17G1262900.v1.1
prunus_persica Prupe.3G164600_v2.0.a1
rosa_chinensis RchiOBHm_Chr3g0492371
rosa_laevigata RLG00000022779
rosa_multiflora Rmu_sc0003156.1_g000020
rosa_roxburghii Rroxscaffold_6G00392380
rosa_samantha Rh3AG303400 Rh3BG339600 Rh3CG336600 Rh3DG338700
rosa_wichuraiana Rw3G026770

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 533
AccII CGCG 2 cut(s) 161, 1244
AccIII TCCGGA 1 cut(s) 254
AclWI GGATC 3 cut(s) 158, 171, 388
AcsI RAATTY 2 cut(s) 236, 1313
AfaI GTAC 3 cut(s) 872, 1094, 1157
AfiI CCNNNNNNNGG 3 cut(s) 182, 317, 424
AflIII ACRYGT 1 cut(s) 1266
AjiI CACGTC 1 cut(s) 937
AjnI CCWGG 1 cut(s) 1006
AluBI AGCT 4 cut(s) 233, 507, 840, 1126
AluI AGCT 4 cut(s) 233, 507, 840, 1126
Alw26I GTCTC 1 cut(s) 1275
AlwI GGATC 3 cut(s) 158, 171, 388
AlwNI CAGNNNCTG 1 cut(s) 892
Aor13HI TCCGGA 1 cut(s) 254
AoxI GGCC 3 cut(s) 3, 1004, 1239
ApeKI GCWGC 5 cut(s) 149, 152, 429, 453, 892
ApoI RAATTY 2 cut(s) 236, 1313
ArsI GACNNNNNNTTYG 2 cut(s) 1253, 1285
AspLEI GCGC 1 cut(s) 122
AspS9I GGNCC 5 cut(s) 513, 827, 1070, 1136, 1239
AsuC2I CCSGG 1 cut(s) 389
AsuHPI GGTGA 2 cut(s) 79, 1058
AvaII GGWCC 4 cut(s) 513, 827, 1070, 1136
BaeGI GKGCMC 1 cut(s) 389
BamHI GGATCC 1 cut(s) 163
BbsI GAAGAC 2 cut(s) 219, 1274
BbvI GCAGC 5 cut(s) 161, 164, 416, 440, 879
BceAI ACGGC 1 cut(s) 880
BcgI CGANNNNNNTGC 2 cut(s) 946, 980
BciT130I CCWGG 1 cut(s) 1008
BciVI GTATCC 1 cut(s) 269
BcnI CCSGG 1 cut(s) 389
BcoDI GTCTC 1 cut(s) 1275
BfmI CTRYAG 1 cut(s) 991
BfuI GTATCC 1 cut(s) 269
BisI GCNGC 8 cut(s) 150, 153, 355, 430, 454, 457, 893, 1242
BlsI GCNGC 8 cut(s) 151, 154, 356, 431, 455, 458, 894, 1243
Bme1390I CCNGG 2 cut(s) 389, 1008
Bme18I GGWCC 4 cut(s) 513, 827, 1070, 1136
BmgBI CACGTC 1 cut(s) 937
BmgT120I GGNCC 5 cut(s) 513, 827, 1070, 1136, 1239
BmiI GGNNCC 4 cut(s) 165, 187, 416, 486
BmrFI CCNGG 2 cut(s) 389, 1008
BmsI GCATC 1 cut(s) 921
BpiI GAAGAC 2 cut(s) 219, 1274
BpmI CTGGAG 2 cut(s) 297, 1193
Bpu10I CCTNAGC 2 cut(s) 875, 1001
BpuEI CTTGAG 2 cut(s) 407, 961
BpuMI CCSGG 1 cut(s) 389
BsaAI YACGTR 2 cut(s) 481, 1201
BsaJI CCNNGG 2 cut(s) 6, 159
BsaWI WCCGGW 2 cut(s) 254, 1138
BsaXI ACNNNNNCTCC 4 cut(s) 249, 278, 279, 308
Bsc4I CCNNNNNNNGG 3 cut(s) 182, 317, 424
Bse118I RCCGGY 1 cut(s) 182
Bse1I ACTGG 3 cut(s) 314, 515, 953
Bse3DI GCAATG 1 cut(s) 843
BseAI TCCGGA 1 cut(s) 254
BseBI CCWGG 1 cut(s) 1008
BseDI CCNNGG 2 cut(s) 6, 159
BseGI GGATG 2 cut(s) 397, 700
BseLI CCNNNNNNNGG 3 cut(s) 182, 317, 424
BseMI GCAATG 1 cut(s) 843
BseMII CTCAG 1 cut(s) 546
BseNI ACTGG 3 cut(s) 314, 515, 953
BseRI GAGGAG 8 cut(s) 31, 91, 254, 338, 407, 410, 687, 1073
BseSI GKGCMC 1 cut(s) 389
BseXI GCAGC 5 cut(s) 161, 164, 416, 440, 879
Bsh1236I CGCG 2 cut(s) 161, 1244
Bsh1285I CGRYCG 1 cut(s) 183
BshFI GGCC 3 cut(s) 5, 1006, 1241
BsiEI CGRYCG 1 cut(s) 183
BsiSI CCGG 4 cut(s) 183, 255, 389, 1139
BslFI GGGAC 1 cut(s) 1240
BslI CCNNNNNNNGG 3 cut(s) 182, 317, 424
BsmAI GTCTC 1 cut(s) 1275
BsmFI GGGAC 1 cut(s) 1240
BsmI GAATGC 1 cut(s) 925
BsnI GGCC 3 cut(s) 5, 1006, 1241
Bsp1286I GDGCHC 1 cut(s) 389
Bsp13I TCCGGA 1 cut(s) 254
Bsp143I GATC 4 cut(s) 163, 343, 380, 1129
BspANI GGCC 3 cut(s) 5, 1006, 1241
BspCNI CTCAG 1 cut(s) 545
BspEI TCCGGA 1 cut(s) 254
BspFNI CGCG 2 cut(s) 161, 1244
BspLI GGNNCC 4 cut(s) 165, 187, 416, 486
BspPI GGATC 3 cut(s) 158, 171, 388
BsrDI GCAATG 1 cut(s) 843
BsrFI RCCGGY 1 cut(s) 182
BsrI ACTGG 3 cut(s) 314, 515, 953
BssAI RCCGGY 1 cut(s) 182
BssECI CCNNGG 2 cut(s) 6, 159
BssMI GATC 4 cut(s) 163, 343, 380, 1129
Bst2UI CCWGG 1 cut(s) 1008
Bst4CI ACNGT 2 cut(s) 522, 614
Bst6I CTCTTC 2 cut(s) 341, 501
BstAPI GCANNNNNTGC 2 cut(s) 892, 931
BstBAI YACGTR 2 cut(s) 481, 1201
BstC8I GCNNGC 1 cut(s) 328
BstDEI CTNAG 3 cut(s) 532, 875, 1001
BstDSI CCRYGG 1 cut(s) 159
BstENI CCTNNNNNAGG 1 cut(s) 422
BstF5I GGATG 2 cut(s) 397, 700
BstFNI CGCG 2 cut(s) 161, 1244
BstHHI GCGC 1 cut(s) 122
BstKTI GATC 4 cut(s) 166, 346, 383, 1132
BstMAI GTCTC 1 cut(s) 1275
BstMBI GATC 4 cut(s) 163, 343, 380, 1129
BstMCI CGRYCG 1 cut(s) 183
BstMWI GCNNNNNNNGC 7 cut(s) 158, 167, 453, 767, 892, 931, 1177
BstNI CCWGG 1 cut(s) 1008
BstNSI RCATGY 2 cut(s) 330, 1270
BstSCI CCNGG 2 cut(s) 387, 1006
BstSFI CTRYAG 1 cut(s) 991
BstSLI GKGCMC 1 cut(s) 389
BstSNI TACGTA 1 cut(s) 1201
BstUI CGCG 2 cut(s) 161, 1244
BstV1I GCAGC 5 cut(s) 161, 164, 416, 440, 879
BstV2I GAAGAC 2 cut(s) 219, 1274
BstX2I RGATCY 1 cut(s) 163
BstYI RGATCY 1 cut(s) 163
BsuI GTATCC 1 cut(s) 269
BsuRI GGCC 3 cut(s) 5, 1006, 1241
BtgI CCRYGG 1 cut(s) 159
BtrI CACGTC 1 cut(s) 937
BtsCI GGATG 2 cut(s) 397, 700
Cac8I GCNNGC 1 cut(s) 328
CaiI CAGNNNCTG 1 cut(s) 892
CfoI GCGC 1 cut(s) 122
Cfr10I RCCGGY 1 cut(s) 182
Cfr13I GGNCC 5 cut(s) 513, 827, 1070, 1136, 1239
Cfr42I CCGCGG 1 cut(s) 162
CseI GACGC 2 cut(s) 240, 459
Csp6I GTAC 3 cut(s) 871, 1093, 1156
CviAII CATG 4 cut(s) 327, 361, 1267, 1278
CviQI GTAC 3 cut(s) 871, 1093, 1156
DdeI CTNAG 3 cut(s) 532, 875, 1001
DpnI GATC 4 cut(s) 165, 345, 382, 1131
DpnII GATC 4 cut(s) 163, 343, 380, 1129
DraI TTTAAA 1 cut(s) 1318
DrdI GACNNNNNNGTC 1 cut(s) 533
DseDI GACNNNNNNGTC 1 cut(s) 533
Eam1104I CTCTTC 2 cut(s) 341, 501
EarI CTCTTC 2 cut(s) 341, 501
EciI GGCGGA 1 cut(s) 341
Eco105I TACGTA 1 cut(s) 1201
Eco147I AGGCCT 1 cut(s) 1006
Eco32I GATATC 1 cut(s) 988
Eco47I GGWCC 4 cut(s) 513, 827, 1070, 1136
EcoNI CCTNNNNNAGG 1 cut(s) 422
EcoRII CCWGG 1 cut(s) 1006
EcoRV GATATC 1 cut(s) 988
FaeI CATG 4 cut(s) 330, 364, 1270, 1281
FaqI GGGAC 1 cut(s) 1240
FatI CATG 4 cut(s) 326, 360, 1266, 1277
FauI CCCGC 2 cut(s) 175, 1004
Fnu4HI GCNGC 8 cut(s) 150, 153, 355, 430, 454, 457, 893, 1242
FokI GGATG 2 cut(s) 404, 707
Fsp4HI GCNGC 8 cut(s) 150, 153, 355, 430, 454, 457, 893, 1242
GlaI GCGC 1 cut(s) 121
GluI GCNGC 8 cut(s) 150, 153, 355, 430, 454, 457, 893, 1242
GsuI CTGGAG 2 cut(s) 297, 1193
HaeIII GGCC 3 cut(s) 5, 1006, 1241
HapII CCGG 4 cut(s) 183, 255, 389, 1139
HgaI GACGC 2 cut(s) 240, 459
HhaI GCGC 1 cut(s) 122
Hin1II CATG 4 cut(s) 330, 364, 1270, 1281
Hin6I GCGC 1 cut(s) 120
HinP1I GCGC 1 cut(s) 120
HindIII AAGCTT 1 cut(s) 1124
HinfI GANTC 5 cut(s) 11, 293, 649, 952, 1300
HpaII CCGG 4 cut(s) 183, 255, 389, 1139
HphI GGTGA 2 cut(s) 79, 1058
Hpy166II GTNNAC 2 cut(s) 516, 1136
Hpy188I TCNGA 3 cut(s) 10, 526, 957
Hpy188III TCNNGA 5 cut(s) 255, 712, 852, 940, 1309
Hpy8I GTNNAC 2 cut(s) 516, 1136
Hpy99I CGWCG 4 cut(s) 453, 611, 961, 964
HpyAV CCTTC 7 cut(s) 74, 141, 236, 641, 775, 1216, 1284
HpyCH4III ACNGT 2 cut(s) 522, 614
HpyCH4IV ACGT 4 cut(s) 480, 936, 962, 1200
HpyCH4V TGCA 5 cut(s) 284, 330, 558, 925, 934
HpyF10VI GCNNNNNNNGC 7 cut(s) 158, 167, 453, 767, 892, 931, 1177
HpyF3I CTNAG 3 cut(s) 532, 875, 1001
HpySE526I ACGT 4 cut(s) 480, 936, 962, 1200
Hsp92II CATG 4 cut(s) 330, 364, 1270, 1281
HspAI GCGC 1 cut(s) 120
Kpn2I TCCGGA 1 cut(s) 254
KspI CCGCGG 1 cut(s) 162
Kzo9I GATC 4 cut(s) 163, 343, 380, 1129
LmnI GCTCC 3 cut(s) 191, 420, 1141
Lsp1109I GCAGC 5 cut(s) 161, 164, 416, 440, 879
LweI GCATC 1 cut(s) 921
MaeII ACGT 4 cut(s) 480, 936, 962, 1200
MaeIII GTNAC 3 cut(s) 944, 1196, 1297
MalI GATC 4 cut(s) 165, 345, 382, 1131
MboI GATC 4 cut(s) 163, 343, 380, 1129
MboII GAAGA 8 cut(s) 224, 328, 488, 658, 1132, 1198, 1217, 1274
MflI RGATCY 1 cut(s) 163
MhlI GDGCHC 1 cut(s) 389
MluCI AATT 4 cut(s) 236, 798, 1291, 1313
MlyI GAGTC 2 cut(s) 287, 1294
MmeI TCCRAC 7 cut(s) 200, 213, 298, 321, 549, 980, 1035
MroI TCCGGA 1 cut(s) 254
MseI TTAA 4 cut(s) 21, 834, 1290, 1317
MspA1I CMGCKG 2 cut(s) 161, 233
MspI CCGG 4 cut(s) 183, 255, 389, 1139
MspR9I CCNGG 2 cut(s) 389, 1008
Mva1269I GAATGC 1 cut(s) 925
MvaI CCWGG 1 cut(s) 1008
MvnI CGCG 2 cut(s) 161, 1244
MwoI GCNNNNNNNGC 7 cut(s) 158, 167, 453, 767, 892, 931, 1177
NciI CCSGG 1 cut(s) 389
NdeII GATC 4 cut(s) 163, 343, 380, 1129
NlaIII CATG 4 cut(s) 330, 364, 1270, 1281
NlaIV GGNNCC 4 cut(s) 165, 187, 416, 486
NmeAIII GCCGAG 1 cut(s) 1142
NmuCI GTSAC 1 cut(s) 1297
NspI RCATGY 2 cut(s) 330, 1270
PaeI GCATGC 1 cut(s) 330
PceI AGGCCT 1 cut(s) 1006
PciI ACATGT 1 cut(s) 1266
PctI GAATGC 1 cut(s) 925
PfeI GAWTC 3 cut(s) 11, 649, 952
PkrI GCNGC 8 cut(s) 151, 154, 356, 431, 455, 458, 894, 1243
PleI GAGTC 2 cut(s) 287, 1294
PpsI GAGTC 2 cut(s) 287, 1294
Ppu21I YACGTR 2 cut(s) 481, 1201
PscI ACATGT 1 cut(s) 1266
Psp6I CCWGG 1 cut(s) 1006
PspGI CCWGG 1 cut(s) 1006
PspN4I GGNNCC 4 cut(s) 165, 187, 416, 486
PspPI GGNCC 5 cut(s) 513, 827, 1070, 1136, 1239
PsrI GAACNNNNNNTAC 2 cut(s) 1181, 1213
PstNI CAGNNNCTG 1 cut(s) 892
PsuI RGATCY 1 cut(s) 163
PvuII CAGCTG 1 cut(s) 233
RsaI GTAC 3 cut(s) 872, 1094, 1157
RsaNI GTAC 3 cut(s) 871, 1093, 1156
SacII CCGCGG 1 cut(s) 162
SaqAI TTAA 4 cut(s) 21, 834, 1290, 1317
SatI GCNGC 8 cut(s) 150, 153, 355, 430, 454, 457, 893, 1242
Sau3AI GATC 4 cut(s) 163, 343, 380, 1129
Sau96I GGNCC 5 cut(s) 513, 827, 1070, 1136, 1239
SchI GAGTC 2 cut(s) 287, 1294
ScrFI CCNGG 2 cut(s) 389, 1008
SduI GDGCHC 1 cut(s) 389
SfaNI GCATC 1 cut(s) 921
SfcI CTRYAG 1 cut(s) 991
Sfr303I CCGCGG 1 cut(s) 162
SgrBI CCGCGG 1 cut(s) 162
SinI GGWCC 4 cut(s) 513, 827, 1070, 1136
SmiI ATTTAAAT 1 cut(s) 1318
SmlI CTYRAG 2 cut(s) 422, 940
SmoI CTYRAG 2 cut(s) 422, 940
SnaBI TACGTA 1 cut(s) 1201
SphI GCATGC 1 cut(s) 330
Sse9I AATT 4 cut(s) 236, 798, 1291, 1313
SseBI AGGCCT 1 cut(s) 1006
SspI AATATT 1 cut(s) 1211
StuI AGGCCT 1 cut(s) 1006
StyD4I CCNGG 2 cut(s) 387, 1006
SwaI ATTTAAAT 1 cut(s) 1318
TaaI ACNGT 2 cut(s) 522, 614
TaiI ACGT 4 cut(s) 483, 939, 965, 1203
TaqI TCGA 4 cut(s) 606, 903, 915, 929
TasI AATT 4 cut(s) 236, 798, 1291, 1313
TauI GCSGC 3 cut(s) 357, 459, 1244
TfiI GAWTC 3 cut(s) 11, 649, 952
Tru1I TTAA 4 cut(s) 21, 834, 1290, 1317
Tru9I TTAA 4 cut(s) 21, 834, 1290, 1317
TseFI GTSAC 1 cut(s) 1297
TseI GCWGC 5 cut(s) 149, 152, 429, 453, 892
Tsp45I GTSAC 1 cut(s) 1297
TspDTI ATGAA 6 cut(s) 349, 794, 830, 832, 959, 1266
TspGWI ACGGA 1 cut(s) 224
VpaK11BI GGWCC 4 cut(s) 513, 827, 1070, 1136
XagI CCTNNNNNAGG 1 cut(s) 422
XapI RAATTY 2 cut(s) 236, 1313
XceI RCATGY 2 cut(s) 330, 1270
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.