FvH4_2g02090

Alkaline neutral invertase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb2
OG0008953
Physical Location & Seq
Forward (+)
1757055 .. 1762073
5019 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_2g02090.t1

Sequence Viewer

Length: 1722 bp
ATGTCATCGTTCAACGGGGATGTGTCTCAGAATGGAAATACAAGACACATGGATTCCCTATTTACTGTGGCAGAAATTGAGGAAATTGATTTCTCAAAGCTTTTAGACAAACCTAAACCTCTGAATATGGAGAGACAACGATCATTTGATGAGAGGTCACTCAGTGAATTATCTGTGGGATTTTCTCCACGTCACTCAGCTCGACATCCGGAGAACTCTTCCAGAAACTATGAACCACCGGAATATTTATTTTCCCCTTGTAGTAGAAGGTCAGTCATTAGTTGTACTCCTAGATCACATGCTGGCTTTGAGCCACATCCGATGGTTGCTGAAGCATGGGAGAATTTGAGGCGTTCATTGGTGTTTTTCCGTGGTGAGCCAGTTGGGACAATTGCTGCAACAGATACATCTGATGAAAAACTGAATTATGATCAGGTGTTTGTGAGAGACTTTGTCCCCAGCGCATTGGCTTTTCTGATGAATGGTGAACCTGAAATTGTTAAAAACTTTATCTTGAAGACTCTTCGTTTGCAGTCATGGGAGAAAAAGATTGATAGGTTCCAGCTAGGAGAAGGAGTAATGCCAGCTAGTTTCAAAGTACTCCATGATCCAGTCAGGAACACTGAGACATTAATGGCAGATTTTGGTGAGAGTGCTATAGGAAGAGTTGCTCCGGTTGATTCTGGGTTTTGGTGGATCATATTGCTTCGCGCATACACAAAATCTACTGGAGACACATCATTGGCTGATCGACCTGAATGCCAGAAGGGTATGCGGCTTATTCTAAGTTTATGTCTTTCGGAGGGCTTCGACACATTTCCAACCCTTCTTTGTGCTGATGGATGCTGCATGATTGATCGAAGAATGGGTGTTTATGGATATCCCATTGAAATTCAGGCTCTTTTCTTCATGGCTTTAAGATGTGCTTTGCTTTTGCTTAAGCAAGATGATGAGGGGAAGGAATTCATTGAGCGCATAGTAAAACGCCTTCATGCCTTGAGCTATCACATGAGAAGTTACTTTTGGCTCGACTTTAAGCAGTTAAATGACATATATCGATATAAAACTGAAGAATACTCACATACAGCTGTCAACAAGTTCAATGTGATACCAGATTCTCTTCCAGACTGGGTCTTTGATTTTATGCCAACACATGGCGGTTACTTTATTGGGAATGTGAGTCCTGCAAGAATGGATTTCCGCTGGTTTTGCTTGGGTAATTGTGTAGCAATTCTTTCATCCTTGGCAACCCCTGAACAATCCATGGCAATCATGGATCTCATAGAGTCACGATGGGAGGAATTGGCAGGAGAAATGCCATTAAAGGTTTGTTATCCAGCCATAGACAGTCATGAATGGAGGATTGAAACAGGAAGTGACCCAAAAAATACTAGATGGAGCTACCACAATGGAGGTTCTTGGCCAGTTCTGCTATGGCTTCTCACTGCTGCATGCATCAAGACTGGACGGCCCCAGATTGCAAGACGAGCTATTGAACTTGCCGAGAGCAGATTGCTGAAAGACAACTGGCCCGAATACTATGATGGGAAATGCGGTCGTTACGTCGGGAAGCAGGCTCGCAAATTCCAGACTTGGTCCATTGCAGGTTACTTGGTGGCCAAGATGATGTTGGAAGACCCATCGCATTTGGGAATGATAGCACTCGAGGAAGACAAACAGATGAAGCCTGCAATGAGGAGATCAAATTCATGGACTTGCTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005829 GO:0044424 GO:0044444 GO:0044464
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Glyco_hydro_100

Protein Analysis

574

Amino Acids

65.78

Weight (kDa)

6.17

Isoelectric Point (pI)

57.43

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Glyco_hydro_100 PF12899 112 - 547 9.1e-220 Alkaline and neutral invertase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 1595
AccB7I CCANNNNNTGG 1 cut(s) 1154
AccII CGCG 1 cut(s) 711
AccIII TCCGGA 1 cut(s) 208
AciI CCGC 4 cut(s) 775, 1158, 1201, 1554
AclWI GGATC 3 cut(s) 602, 704, 1284
AcoI YGGCCR 2 cut(s) 1421, 1617
AcsI RAATTY 5 cut(s) 343, 891, 962, 1583, 1705
AcuI CTGAAG 2 cut(s) 351, 1089
AdeI CACNNNGTG 1 cut(s) 164
AfaI GTAC 2 cut(s) 286, 600
AfiI CCNNNNNNNGG 1 cut(s) 1154
AflII CTTAAG 1 cut(s) 938
AgsI TTSAA 7 cut(s) 13, 517, 595, 890, 1102, 1367, 1496
AjiI CACGTC 1 cut(s) 191
AjuI GAANNNNNNNTTGG 2 cut(s) 1006, 1038
AluBI AGCT 8 cut(s) 100, 200, 565, 587, 1002, 1088, 1401, 1490
AluI AGCT 8 cut(s) 100, 200, 565, 587, 1002, 1088, 1401, 1490
Alw26I GTCTC 5 cut(s) 30, 127, 441, 620, 726
AlwI GGATC 3 cut(s) 602, 704, 1284
Ama87I CYCGRG 1 cut(s) 1664
Aor13HI TCCGGA 1 cut(s) 208
AoxI GGCC 4 cut(s) 1421, 1469, 1529, 1617
ApeKI GCWGC 3 cut(s) 395, 846, 1448
ApoI RAATTY 5 cut(s) 343, 891, 962, 1583, 1705
ArsI GACNNNNNNTTYG 4 cut(s) 511, 543, 1118, 1150
AseI ATTAAT 1 cut(s) 632
Asp700I GAANNNNTTC 1 cut(s) 962
AspLEI GCGC 3 cut(s) 464, 713, 975
AspS9I GGNCC 3 cut(s) 1470, 1530, 1596
AsuHPI GGTGA 3 cut(s) 386, 497, 659
AvaI CYCGRG 1 cut(s) 1664
AvaII GGWCC 1 cut(s) 1596
BalI TGGCCA 2 cut(s) 1423, 1619
BarI GAAGNNNNNNTAC 2 cut(s) 972, 1004
BbsI GAAGAC 3 cut(s) 524, 1641, 1677
BbvI GCAGC 3 cut(s) 382, 833, 1435
BccI CCATC 6 cut(s) 316, 833, 1287, 1389, 1538, 1648
BceAI ACGGC 1 cut(s) 1484
BcgI CGANNNNNNTGC 2 cut(s) 741, 775
BclI TGATCA 1 cut(s) 430
BcoDI GTCTC 5 cut(s) 30, 127, 441, 620, 726
BfaI CTAG 4 cut(s) 291, 566, 588, 1392
BfmI CTRYAG 1 cut(s) 657
BfrI CTTAAG 1 cut(s) 938
BfuAI ACCTGC 1 cut(s) 1595
BisI GCNGC 4 cut(s) 396, 776, 847, 1449
BlsI GCNGC 4 cut(s) 397, 777, 848, 1450
BmcAI AGTACT 1 cut(s) 600
Bme18I GGWCC 1 cut(s) 1596
BmeT110I CYCGRG 1 cut(s) 1664
BmgBI CACGTC 1 cut(s) 191
BmgT120I GGNCC 3 cut(s) 1470, 1530, 1596
BmiI GGNNCC 2 cut(s) 560, 1472
BmrI ACTGGG 1 cut(s) 1138
BmsI GCATC 2 cut(s) 833, 1464
BmuI ACTGGG 1 cut(s) 1138
BpiI GAAGAC 3 cut(s) 524, 1641, 1677
BpmI CTGGAG 1 cut(s) 750
BpuEI CTTGAG 1 cut(s) 1018
Bsa29I ATCGAT 1 cut(s) 1057
BsaJI CCNNGG 3 cut(s) 370, 1242, 1263
BsaWI WCCGGW 3 cut(s) 208, 238, 673
BsaXI ACNNNNNCTCC 2 cut(s) 561, 591
Bsc4I CCNNNNNNNGG 1 cut(s) 1154
Bse1I ACTGG 7 cut(s) 380, 611, 733, 1133, 1424, 1468, 1532
Bse3DI GCAATG 2 cut(s) 1599, 1698
BseAI TCCGGA 1 cut(s) 208
BseCI ATCGAT 1 cut(s) 1057
BseDI CCNNGG 3 cut(s) 370, 1242, 1263
BseGI GGATG 5 cut(s) 25, 205, 316, 848, 1238
BseLI CCNNNNNNNGG 1 cut(s) 1154
BseMI GCAATG 2 cut(s) 1599, 1698
BseMII CTCAG 4 cut(s) 41, 175, 210, 615
BseNI ACTGG 7 cut(s) 380, 611, 733, 1133, 1424, 1468, 1532
BseRI GAGGAG 1 cut(s) 1711
BseXI GCAGC 3 cut(s) 382, 833, 1435
BseYI CCCAGC 1 cut(s) 458
Bsh1236I CGCG 1 cut(s) 711
Bsh1285I CGRYCG 1 cut(s) 1558
BshFI GGCC 4 cut(s) 1423, 1471, 1531, 1619
BshVI ATCGAT 1 cut(s) 1057
BsiEI CGRYCG 1 cut(s) 1558
BsiHKCI CYCGRG 1 cut(s) 1664
BsiSI CCGG 3 cut(s) 209, 239, 674
BslFI GGGAC 2 cut(s) 400, 440
BslI CCNNNNNNNGG 1 cut(s) 1154
BsmAI GTCTC 5 cut(s) 30, 127, 441, 620, 726
BsmFI GGGAC 2 cut(s) 400, 440
BsmI GAATGC 1 cut(s) 764
BsnI GGCC 4 cut(s) 1423, 1471, 1531, 1619
BsoBI CYCGRG 1 cut(s) 1664
Bsp13I TCCGGA 1 cut(s) 208
Bsp143I GATC 9 cut(s) 140, 293, 430, 607, 696, 748, 856, 1276, 1700
Bsp19I CCATGG 1 cut(s) 1263
BspACI CCGC 4 cut(s) 775, 1158, 1201, 1554
BspANI GGCC 4 cut(s) 1423, 1471, 1531, 1619
BspCNI CTCAG 4 cut(s) 40, 174, 209, 616
BspDI ATCGAT 1 cut(s) 1057
BspEI TCCGGA 1 cut(s) 208
BspFNI CGCG 1 cut(s) 711
BspHI TCATGA 1 cut(s) 1351
BspLI GGNNCC 2 cut(s) 560, 1472
BspMI ACCTGC 1 cut(s) 1595
BspPI GGATC 3 cut(s) 602, 704, 1284
BspTI CTTAAG 1 cut(s) 938
BsrDI GCAATG 2 cut(s) 1599, 1698
BsrI ACTGG 7 cut(s) 380, 611, 733, 1133, 1424, 1468, 1532
BssECI CCNNGG 3 cut(s) 370, 1242, 1263
BssMI GATC 9 cut(s) 140, 293, 430, 607, 696, 748, 856, 1276, 1700
BssT1I CCWWGG 2 cut(s) 1242, 1263
Bst4CI ACNGT 2 cut(s) 67, 1349
Bst6I CTCTTC 4 cut(s) 223, 528, 658, 1125
BstAFI CTTAAG 1 cut(s) 938
BstC8I GCNNGC 6 cut(s) 304, 585, 1453, 1575, 1579, 1689
BstDEI CTNAG 5 cut(s) 27, 161, 196, 624, 785
BstDSI CCRYGG 2 cut(s) 370, 1263
BstF5I GGATG 5 cut(s) 25, 205, 316, 848, 1238
BstFNI CGCG 1 cut(s) 711
BstHHI GCGC 3 cut(s) 464, 713, 975
BstKTI GATC 9 cut(s) 143, 296, 433, 610, 699, 751, 859, 1279, 1703
BstMAI GTCTC 5 cut(s) 30, 127, 441, 620, 726
BstMBI GATC 9 cut(s) 140, 293, 430, 607, 696, 748, 856, 1276, 1700
BstMCI CGRYCG 1 cut(s) 1558
BstMWI GCNNNNNNNGC 3 cut(s) 1209, 1429, 1487
BstNSI RCATGY 2 cut(s) 302, 1455
BstSFI CTRYAG 1 cut(s) 657
BstUI CGCG 1 cut(s) 711
BstV1I GCAGC 3 cut(s) 382, 833, 1435
BstV2I GAAGAC 3 cut(s) 524, 1641, 1677
BstX2I RGATCY 1 cut(s) 1276
BstXI CCANNNNNNTGG 1 cut(s) 466
BstYI RGATCY 1 cut(s) 1276
Bsu15I ATCGAT 1 cut(s) 1057
BsuRI GGCC 4 cut(s) 1423, 1471, 1531, 1619
BsuTUI ATCGAT 1 cut(s) 1057
BtgI CCRYGG 2 cut(s) 370, 1263
BtgZI GCGATG 1 cut(s) 1626
BtrI CACGTC 1 cut(s) 191
BtsCI GGATG 5 cut(s) 25, 205, 316, 848, 1238
BtsI GCAGTG 1 cut(s) 1443
BtsIMutI CAGTG 3 cut(s) 169, 621, 1443
BveI ACCTGC 1 cut(s) 1595
Cac8I GCNNGC 6 cut(s) 304, 585, 1453, 1575, 1579, 1689
CciI TCATGA 1 cut(s) 1351
CfoI GCGC 3 cut(s) 464, 713, 975
Cfr13I GGNCC 3 cut(s) 1470, 1530, 1596
ClaI ATCGAT 1 cut(s) 1057
Csp6I GTAC 2 cut(s) 285, 599
CviQI GTAC 2 cut(s) 285, 599
DdeI CTNAG 5 cut(s) 27, 161, 196, 624, 785
DpnI GATC 9 cut(s) 142, 295, 432, 609, 698, 750, 858, 1278, 1702
DpnII GATC 9 cut(s) 140, 293, 430, 607, 696, 748, 856, 1276, 1700
DraIII CACNNNGTG 1 cut(s) 164
EaeI YGGCCR 2 cut(s) 1421, 1617
Eam1104I CTCTTC 4 cut(s) 223, 528, 658, 1125
EarI CTCTTC 4 cut(s) 223, 528, 658, 1125
Eco130I CCWWGG 2 cut(s) 1242, 1263
Eco32I GATATC 1 cut(s) 881
Eco47I GGWCC 1 cut(s) 1596
Eco57I CTGAAG 2 cut(s) 351, 1089
Eco88I CYCGRG 1 cut(s) 1664
EcoRI GAATTC 1 cut(s) 962
EcoRV GATATC 1 cut(s) 881
EcoT14I CCWWGG 2 cut(s) 1242, 1263
EcoT22I ATGCAT 1 cut(s) 1457
ErhI CCWWGG 2 cut(s) 1242, 1263
FalI AAGNNNNNCTT 2 cut(s) 910, 942
FaqI GGGAC 2 cut(s) 400, 440
FbaI TGATCA 1 cut(s) 430
Fnu4HI GCNGC 4 cut(s) 396, 776, 847, 1449
FokI GGATG 5 cut(s) 32, 192, 303, 855, 1225
Fsp4HI GCNGC 4 cut(s) 396, 776, 847, 1449
FspBI CTAG 4 cut(s) 291, 566, 588, 1392
GlaI GCGC 3 cut(s) 463, 712, 974
GluI GCNGC 4 cut(s) 396, 776, 847, 1449
GsaI CCCAGC 1 cut(s) 462
GsuI CTGGAG 1 cut(s) 750
HaeIII GGCC 4 cut(s) 1423, 1471, 1531, 1619
HapII CCGG 3 cut(s) 209, 239, 674
HhaI GCGC 3 cut(s) 464, 713, 975
Hin6I GCGC 3 cut(s) 462, 711, 973
HinP1I GCGC 3 cut(s) 462, 711, 973
HincII GTYRAC 1 cut(s) 1093
HindII GTYRAC 1 cut(s) 1093
HindIII AAGCTT 1 cut(s) 98
HinfI GANTC 6 cut(s) 53, 520, 680, 1115, 1180, 1286
HpaII CCGG 3 cut(s) 209, 239, 674
HphI GGTGA 3 cut(s) 386, 497, 659
Hpy166II GTNNAC 2 cut(s) 488, 1093
Hpy188I TCNGA 6 cut(s) 30, 123, 321, 412, 477, 802
Hpy8I GTNNAC 2 cut(s) 488, 1093
Hpy99I CGWCG 1 cut(s) 1568
HpyAV CCTTC 6 cut(s) 261, 566, 760, 836, 952, 998
HpyCH4III ACNGT 2 cut(s) 67, 1349
HpyCH4IV ACGT 2 cut(s) 190, 1563
HpyCH4V TGCA 9 cut(s) 398, 532, 849, 1187, 1451, 1455, 1481, 1604, 1691
HpyF10VI GCNNNNNNNGC 3 cut(s) 1209, 1429, 1487
HpyF3I CTNAG 5 cut(s) 27, 161, 196, 624, 785
HpySE526I ACGT 2 cut(s) 190, 1563
HspAI GCGC 3 cut(s) 462, 711, 973
Kpn2I TCCGGA 1 cut(s) 208
Ksp22I TGATCA 1 cut(s) 430
Kzo9I GATC 9 cut(s) 140, 293, 430, 607, 696, 748, 856, 1276, 1700
LmnI GCTCC 2 cut(s) 676, 1398
Lsp1109I GCAGC 3 cut(s) 382, 833, 1435
LweI GCATC 2 cut(s) 833, 1464
MaeI CTAG 4 cut(s) 291, 566, 588, 1392
MaeII ACGT 2 cut(s) 190, 1563
MaeIII GTNAC 8 cut(s) 156, 191, 1016, 1160, 1287, 1376, 1559, 1607
MalI GATC 9 cut(s) 142, 295, 432, 609, 698, 750, 858, 1278, 1702
MboI GATC 9 cut(s) 140, 293, 430, 607, 696, 748, 856, 1276, 1700
MfeI CAATTG 1 cut(s) 390
MflI RGATCY 1 cut(s) 1276
MlsI TGGCCA 2 cut(s) 1423, 1619
MluNI TGGCCA 2 cut(s) 1423, 1619
MlyI GAGTC 3 cut(s) 514, 1189, 1295
MmeI TCCRAC 2 cut(s) 845, 1611
Mox20I TGGCCA 2 cut(s) 1423, 1619
Mph1103I ATGCAT 1 cut(s) 1457
MroI TCCGGA 1 cut(s) 208
MroXI GAANNNNTTC 1 cut(s) 962
MscI TGGCCA 2 cut(s) 1423, 1619
MseI TTAA 7 cut(s) 501, 632, 917, 939, 1035, 1043, 1322
Msp20I TGGCCA 2 cut(s) 1423, 1619
MspA1I CMGCKG 2 cut(s) 1088, 1203
MspCI CTTAAG 1 cut(s) 938
MspI CCGG 3 cut(s) 209, 239, 674
MunI CAATTG 1 cut(s) 390
Mva1269I GAATGC 1 cut(s) 764
MvnI CGCG 1 cut(s) 711
MwoI GCNNNNNNNGC 3 cut(s) 1209, 1429, 1487
NcoI CCATGG 1 cut(s) 1263
NdeII GATC 9 cut(s) 140, 293, 430, 607, 696, 748, 856, 1276, 1700
NlaIV GGNNCC 2 cut(s) 560, 1472
NmeAIII GCCGAG 1 cut(s) 1528
NmuCI GTSAC 4 cut(s) 156, 191, 1287, 1376
NsiI ATGCAT 1 cut(s) 1457
NspI RCATGY 2 cut(s) 302, 1455
PaeI GCATGC 1 cut(s) 1455
PaeR7I CTCGAG 1 cut(s) 1664
PagI TCATGA 1 cut(s) 1351
PctI GAATGC 1 cut(s) 764
PdmI GAANNNNTTC 1 cut(s) 962
PfeI GAWTC 3 cut(s) 53, 680, 1115
PflFI GACNNNGTC 3 cut(s) 452, 1130, 1594
PflMI CCANNNNNTGG 1 cut(s) 1154
PkrI GCNGC 4 cut(s) 397, 777, 848, 1450
PleI GAGTC 3 cut(s) 514, 1188, 1294
PpsI GAGTC 3 cut(s) 514, 1188, 1294
PshBI ATTAAT 1 cut(s) 632
PspFI CCCAGC 1 cut(s) 458
PspN4I GGNNCC 2 cut(s) 560, 1472
PspPI GGNCC 3 cut(s) 1470, 1530, 1596
PspXI VCTCGAGB 1 cut(s) 1664
PsuI RGATCY 1 cut(s) 1276
PsyI GACNNNGTC 3 cut(s) 452, 1130, 1594
PvuII CAGCTG 1 cut(s) 1088
RsaI GTAC 2 cut(s) 286, 600
RsaNI GTAC 2 cut(s) 285, 599
SaqAI TTAA 7 cut(s) 501, 632, 917, 939, 1035, 1043, 1322
SatI GCNGC 4 cut(s) 396, 776, 847, 1449
Sau3AI GATC 9 cut(s) 140, 293, 430, 607, 696, 748, 856, 1276, 1700
Sau96I GGNCC 3 cut(s) 1470, 1530, 1596
ScaI AGTACT 1 cut(s) 600
SchI GAGTC 3 cut(s) 514, 1189, 1295
SfaNI GCATC 2 cut(s) 833, 1464
SfcI CTRYAG 1 cut(s) 657
Sfr274I CTCGAG 1 cut(s) 1664
SinI GGWCC 1 cut(s) 1596
SlaI CTCGAG 1 cut(s) 1664
SmlI CTYRAG 3 cut(s) 938, 997, 1664
SmoI CTYRAG 3 cut(s) 938, 997, 1664
SphI GCATGC 1 cut(s) 1455
SsiI CCGC 4 cut(s) 775, 1158, 1201, 1554
SspI AATATT 1 cut(s) 245
SspMI CTAG 4 cut(s) 291, 566, 588, 1392
StyI CCWWGG 2 cut(s) 1242, 1263
TaaI ACNGT 2 cut(s) 67, 1349
TaiI ACGT 2 cut(s) 193, 1566
TaqI TCGA 7 cut(s) 202, 751, 810, 859, 1029, 1057, 1665
TatI WGTACW 2 cut(s) 284, 598
TauI GCSGC 1 cut(s) 778
TfiI GAWTC 3 cut(s) 53, 680, 1115
Tru1I TTAA 7 cut(s) 501, 632, 917, 939, 1035, 1043, 1322
Tru9I TTAA 7 cut(s) 501, 632, 917, 939, 1035, 1043, 1322
TscAI CASTG 3 cut(s) 169, 628, 1450
TseFI GTSAC 4 cut(s) 156, 191, 1287, 1376
TseI GCWGC 3 cut(s) 395, 846, 1448
Tsp45I GTSAC 4 cut(s) 156, 191, 1287, 1376
TspGWI ACGGA 1 cut(s) 359
TspRI CASTG 3 cut(s) 169, 628, 1450
Tth111I GACNNNGTC 3 cut(s) 452, 1130, 1594
Van91I CCANNNNNTGG 1 cut(s) 1154
Vha464I CTTAAG 1 cut(s) 938
VpaK11BI GGWCC 1 cut(s) 1596
VspI ATTAAT 1 cut(s) 632
XapI RAATTY 5 cut(s) 343, 891, 962, 1583, 1705
XceI RCATGY 2 cut(s) 302, 1455
XcmI CCANNNNNNNNNTGG 3 cut(s) 1270, 1431, 1627
XhoI CTCGAG 1 cut(s) 1664
XmnI GAANNNNTTC 1 cut(s) 962
XspI CTAG 4 cut(s) 291, 566, 588, 1392
ZrmI AGTACT 1 cut(s) 600
Zsp2I ATGCAT 1 cut(s) 1457
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.