Rroxscaffold_7G00215240

Alkaline neutral invertase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000007
OG0008953
Physical Location & Seq
Reverse (-)
65857655 .. 65861519
3865 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_7G00215240.1

Sequence Viewer

Length: 1623 bp
ATGTCATCACTGAATGGGGATGTGTCTCAGAATGGAAATACAAGAAATATGGATTCCCTATTTACTGTGGCAGAAATTGAGGAAGTTGATTTCTCAAAGCTATTGGACAGACCTAAACCTCTGAATATTGAGAGACAACGATCATTTGATGAGAGGTCACTCAGTGAATTATCTGTGGGGTTTTCTCCACGCCTCTCAGCTCGACATCCGGAGAACTCTCTTAGGAACTATGACCCACATGAATATTTATTTTCCCCCTGTAGTAGAAGATTCAGTTCTGTGAAGTTGGCAAGCACTGATTTTCTACTTGTTTTTTCTTCTTATGAACCTCTGAAGGTGTTTGTGAGAGACTTTGTCCCCAGCGCATTGGCTTTTCTGATGAATGGTGAACCTGAAATTGTTAAAAACTTTATCTTGAAGACTCTCCGTCTGCAGTCATGGGAGAAAAAGATTGATAGATTCCAGCTAGGAGAAGGAGTAATGCCAGCTAGTTTCAAAGTACTCCATGATCCAGTCAGGAACACTGAGACTTTAATGGCAGATTTCGGTGAGAGTGCTATAGGAAGAGTTGCTCCGGTTGATTCTGGATTTTGGTGGATCATATTGCTTCGCGCATACACAAAATCTACTGGAGACACATCATTGGCTGAACGGCCTGAATGCCAGAAGGGTATGCGGCTTATTCTAAGTTTATGTCTTTCGGAAGGGTTCGACACATTTCCAACCCTTCTTTGTGCTGACGGATGCTGCATGATTGATCGTAGAATGGGTGTTTATGGATATCCCATTGAAATTCAGGCTCTTTTCTTCATGGCTTTAAGATGTGCTTTGCTTTTACTTAACAAAGATGATGAGGGGAAGGAGTTCATTGAGCGAATAGTAAAACGCCTTCATGCCTTGAGCTATCACATGAGAAGTTACTTTTGGCTTGACTTTAAGCAGTTAAATGATATATATCGATATAAAACTGAAGAATACTCGCATACAGCCGTCAACAAGTTCAACGTGATACCAGATTCTCTTCCGGACTGGGTCTTTGATTTTATGCCAACACACGGTGGTTACTTTATTGGGAATGTGAGTCCTGCAAGAATGGATTTCCGCTGGTTTTGCTTGGGTAATTGTATAGCGATTCTTTCATCCTTGGCAACCCCTGAACAATCCATGGCAATCATGGATCTCATAGAGTCACGATGGGAGGAATTGGCAGGAGAAATGCCATTAAAAGTTTGTTATCCAGCCATAGACAGTCATGAATGGAGGATTGAAACAGGAAGTGACCCAAAAAATACTAGATGGAGCTACCACAATGGAGGTTCTTGGCCAGTTCTGCTATGGCTTCTCACTGCTGCATGCATCAAGACTGGACGGCCCCAGATTGCAAGACGAGCTATTGAGCTTGCCGAGAGCAGGTTGCAGAAAGACAACTGGCCCGAATACTATGATGGAAAATGTGGTCGTTACGTTGGGAAGCAGGCTCGCAAATTCCAGACTTGGTCCATTGCAGGTTACTTGGTGGCCAAGATGATGTTGGAAGACCCATCCCATTTGGGAATGATAGCGCTCGAGGAAGACAAACAGATGAAGCCTGCAATGAGGAGATCGAATTCATGGACTTGCTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005829 GO:0044424 GO:0044444 GO:0044464
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Glyco_hydro_100

Protein Analysis

540

Amino Acids

62.12

Weight (kDa)

6.41

Isoelectric Point (pI)

56.75

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Glyco_hydro_100 PF12899 112 - 514 1.3e-205 Alkaline and neutral invertase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 2 cut(s) 1401, 1496
AccII CGCG 1 cut(s) 612
AccIII TCCGGA 2 cut(s) 208, 1024
AciI CCGC 2 cut(s) 676, 1102
AclWI GGATC 3 cut(s) 503, 605, 1185
AcoI YGGCCR 2 cut(s) 1322, 1518
AcsI RAATTY 3 cut(s) 792, 1484, 1606
AcuI CTGAAG 2 cut(s) 353, 990
AdeI CACNNNGTG 2 cut(s) 164, 1058
AfaI GTAC 1 cut(s) 501
AfeI AGCGCT 1 cut(s) 1563
AfiI CCNNNNNNNGG 2 cut(s) 1055, 1410
AgsI TTSAA 5 cut(s) 418, 496, 791, 1003, 1268
AhdI GACNNNNNGTC 1 cut(s) 426
AjuI GAANNNNNNNTTGG 2 cut(s) 907, 939
AluBI AGCT 8 cut(s) 100, 200, 466, 488, 903, 1302, 1391, 1399
AluI AGCT 8 cut(s) 100, 200, 466, 488, 903, 1302, 1391, 1399
Alw26I GTCTC 5 cut(s) 30, 127, 342, 521, 627
AlwI GGATC 3 cut(s) 503, 605, 1185
Ama87I CYCGRG 1 cut(s) 1565
Aor13HI TCCGGA 2 cut(s) 208, 1024
Aor51HI AGCGCT 1 cut(s) 1563
AoxI GGCC 5 cut(s) 653, 1322, 1370, 1430, 1518
ApeKI GCWGC 2 cut(s) 747, 1349
ApoI RAATTY 3 cut(s) 792, 1484, 1606
ArsI GACNNNNNNTTYG 2 cut(s) 1019, 1051
Asp700I GAANNNNTTC 1 cut(s) 863
AspLEI GCGC 3 cut(s) 365, 614, 1564
AspS9I GGNCC 3 cut(s) 1371, 1431, 1497
AsuHPI GGTGA 2 cut(s) 398, 560
AvaI CYCGRG 1 cut(s) 1565
AvaII GGWCC 1 cut(s) 1497
BalI TGGCCA 2 cut(s) 1324, 1520
BarI GAAGNNNNNNTAC 2 cut(s) 873, 905
BbsI GAAGAC 3 cut(s) 425, 1542, 1578
BbvI GCAGC 2 cut(s) 734, 1336
BccI CCATC 4 cut(s) 1188, 1290, 1439, 1549
BceAI ACGGC 3 cut(s) 668, 974, 1385
BcoDI GTCTC 5 cut(s) 30, 127, 342, 521, 627
BfaI CTAG 3 cut(s) 467, 489, 1293
BfmI CTRYAG 3 cut(s) 259, 431, 558
BfoI RGCGCY 1 cut(s) 1565
BfuAI ACCTGC 2 cut(s) 1401, 1496
BisI GCNGC 3 cut(s) 677, 748, 1350
BlsI GCNGC 3 cut(s) 678, 749, 1351
BmcAI AGTACT 1 cut(s) 501
Bme18I GGWCC 1 cut(s) 1497
BmeRI GACNNNNNGTC 1 cut(s) 426
BmeT110I CYCGRG 1 cut(s) 1565
BmgT120I GGNCC 3 cut(s) 1371, 1431, 1497
BmiI GGNNCC 1 cut(s) 1373
BmrI ACTGGG 1 cut(s) 1039
BmsI GCATC 2 cut(s) 734, 1365
BmuI ACTGGG 1 cut(s) 1039
BpiI GAAGAC 3 cut(s) 425, 1542, 1578
BpmI CTGGAG 1 cut(s) 651
BpuEI CTTGAG 1 cut(s) 919
Bsa29I ATCGAT 1 cut(s) 958
BsaBI GATNNNNATC 1 cut(s) 954
BsaJI CCNNGG 2 cut(s) 1143, 1164
BsaWI WCCGGW 3 cut(s) 208, 574, 1024
BsaXI ACNNNNNCTCC 2 cut(s) 462, 492
Bsc4I CCNNNNNNNGG 2 cut(s) 1055, 1410
Bse1I ACTGG 6 cut(s) 512, 634, 1034, 1325, 1369, 1433
Bse3DI GCAATG 2 cut(s) 1500, 1599
Bse8I GATNNNNATC 1 cut(s) 954
BseAI TCCGGA 2 cut(s) 208, 1024
BseCI ATCGAT 1 cut(s) 958
BseDI CCNNGG 2 cut(s) 1143, 1164
BseGI GGATG 5 cut(s) 25, 205, 749, 1139, 1541
BseJI GATNNNNATC 1 cut(s) 954
BseLI CCNNNNNNNGG 2 cut(s) 1055, 1410
BseMI GCAATG 2 cut(s) 1500, 1599
BseMII CTCAG 4 cut(s) 41, 175, 210, 516
BseNI ACTGG 6 cut(s) 512, 634, 1034, 1325, 1369, 1433
BseRI GAGGAG 1 cut(s) 1612
BseXI GCAGC 2 cut(s) 734, 1336
BseYI CCCAGC 1 cut(s) 359
Bsh1236I CGCG 1 cut(s) 612
BshFI GGCC 5 cut(s) 655, 1324, 1372, 1432, 1520
BshVI ATCGAT 1 cut(s) 958
BsiHKCI CYCGRG 1 cut(s) 1565
BsiSI CCGG 3 cut(s) 209, 575, 1025
BslFI GGGAC 1 cut(s) 341
BslI CCNNNNNNNGG 2 cut(s) 1055, 1410
BsmAI GTCTC 5 cut(s) 30, 127, 342, 521, 627
BsmFI GGGAC 1 cut(s) 341
BsmI GAATGC 1 cut(s) 665
BsnI GGCC 5 cut(s) 655, 1324, 1372, 1432, 1520
BsoBI CYCGRG 1 cut(s) 1565
Bsp13I TCCGGA 2 cut(s) 208, 1024
Bsp143I GATC 6 cut(s) 140, 508, 597, 757, 1177, 1601
Bsp19I CCATGG 1 cut(s) 1164
BspACI CCGC 2 cut(s) 676, 1102
BspANI GGCC 5 cut(s) 655, 1324, 1372, 1432, 1520
BspCNI CTCAG 4 cut(s) 40, 174, 209, 517
BspDI ATCGAT 1 cut(s) 958
BspEI TCCGGA 2 cut(s) 208, 1024
BspFNI CGCG 1 cut(s) 612
BspHI TCATGA 1 cut(s) 1252
BspLI GGNNCC 1 cut(s) 1373
BspMAI CTGCAG 1 cut(s) 435
BspMI ACCTGC 2 cut(s) 1401, 1496
BspPI GGATC 3 cut(s) 503, 605, 1185
BsrDI GCAATG 2 cut(s) 1500, 1599
BsrI ACTGG 6 cut(s) 512, 634, 1034, 1325, 1369, 1433
BssECI CCNNGG 2 cut(s) 1143, 1164
BssMI GATC 6 cut(s) 140, 508, 597, 757, 1177, 1601
BssT1I CCWWGG 2 cut(s) 1143, 1164
Bst4CI ACNGT 3 cut(s) 67, 1058, 1250
Bst6I CTCTTC 2 cut(s) 559, 1026
BstC8I GCNNGC 7 cut(s) 292, 486, 1354, 1401, 1476, 1480, 1590
BstDEI CTNAG 6 cut(s) 27, 161, 196, 221, 525, 686
BstDSI CCRYGG 1 cut(s) 1164
BstF5I GGATG 5 cut(s) 25, 205, 749, 1139, 1541
BstFNI CGCG 1 cut(s) 612
BstH2I RGCGCY 1 cut(s) 1565
BstHHI GCGC 3 cut(s) 365, 614, 1564
BstKTI GATC 6 cut(s) 143, 511, 600, 760, 1180, 1604
BstMAI GTCTC 5 cut(s) 30, 127, 342, 521, 627
BstMBI GATC 6 cut(s) 140, 508, 597, 757, 1177, 1601
BstMWI GCNNNNNNNGC 3 cut(s) 1110, 1330, 1388
BstNSI RCATGY 1 cut(s) 1356
BstSFI CTRYAG 3 cut(s) 259, 431, 558
BstUI CGCG 1 cut(s) 612
BstV1I GCAGC 2 cut(s) 734, 1336
BstV2I GAAGAC 3 cut(s) 425, 1542, 1578
BstX2I RGATCY 1 cut(s) 1177
BstXI CCANNNNNNTGG 1 cut(s) 367
BstYI RGATCY 1 cut(s) 1177
Bsu15I ATCGAT 1 cut(s) 958
BsuRI GGCC 5 cut(s) 655, 1324, 1372, 1432, 1520
BsuTUI ATCGAT 1 cut(s) 958
BtgI CCRYGG 1 cut(s) 1164
BtsCI GGATG 5 cut(s) 25, 205, 749, 1139, 1541
BtsI GCAGTG 1 cut(s) 1344
BtsIMutI CAGTG 5 cut(s) 8, 169, 294, 522, 1344
BveI ACCTGC 2 cut(s) 1401, 1496
Cac8I GCNNGC 7 cut(s) 292, 486, 1354, 1401, 1476, 1480, 1590
CciI TCATGA 1 cut(s) 1252
CfoI GCGC 3 cut(s) 365, 614, 1564
Cfr13I GGNCC 3 cut(s) 1371, 1431, 1497
ClaI ATCGAT 1 cut(s) 958
Csp6I GTAC 1 cut(s) 500
CviQI GTAC 1 cut(s) 500
DdeI CTNAG 6 cut(s) 27, 161, 196, 221, 525, 686
DpnI GATC 6 cut(s) 142, 510, 599, 759, 1179, 1603
DpnII GATC 6 cut(s) 140, 508, 597, 757, 1177, 1601
DraIII CACNNNGTG 2 cut(s) 164, 1058
DriI GACNNNNNGTC 1 cut(s) 426
EaeI YGGCCR 2 cut(s) 1322, 1518
Eam1104I CTCTTC 2 cut(s) 559, 1026
Eam1105I GACNNNNNGTC 1 cut(s) 426
EarI CTCTTC 2 cut(s) 559, 1026
Eco130I CCWWGG 2 cut(s) 1143, 1164
Eco32I GATATC 1 cut(s) 782
Eco47I GGWCC 1 cut(s) 1497
Eco47III AGCGCT 1 cut(s) 1563
Eco57I CTGAAG 2 cut(s) 353, 990
Eco88I CYCGRG 1 cut(s) 1565
EcoRI GAATTC 1 cut(s) 1606
EcoRV GATATC 1 cut(s) 782
EcoT14I CCWWGG 2 cut(s) 1143, 1164
EcoT22I ATGCAT 1 cut(s) 1358
ErhI CCWWGG 2 cut(s) 1143, 1164
FalI AAGNNNNNCTT 2 cut(s) 811, 843
FaqI GGGAC 1 cut(s) 341
Fnu4HI GCNGC 3 cut(s) 677, 748, 1350
FokI GGATG 5 cut(s) 32, 192, 756, 1126, 1528
Fsp4HI GCNGC 3 cut(s) 677, 748, 1350
FspBI CTAG 3 cut(s) 467, 489, 1293
GlaI GCGC 3 cut(s) 364, 613, 1563
GluI GCNGC 3 cut(s) 677, 748, 1350
GsaI CCCAGC 1 cut(s) 363
GsuI CTGGAG 1 cut(s) 651
HaeII RGCGCY 1 cut(s) 1565
HaeIII GGCC 5 cut(s) 655, 1324, 1372, 1432, 1520
HapII CCGG 3 cut(s) 209, 575, 1025
HhaI GCGC 3 cut(s) 365, 614, 1564
Hin6I GCGC 3 cut(s) 363, 612, 1562
HinP1I GCGC 3 cut(s) 363, 612, 1562
HincII GTYRAC 1 cut(s) 994
HindII GTYRAC 1 cut(s) 994
HinfI GANTC 9 cut(s) 53, 270, 421, 459, 581, 1016, 1081, 1132, 1187
HpaII CCGG 3 cut(s) 209, 575, 1025
HphI GGTGA 2 cut(s) 398, 560
Hpy166II GTNNAC 2 cut(s) 389, 994
Hpy188I TCNGA 5 cut(s) 30, 123, 333, 378, 703
Hpy188III TCNNGA 9 cut(s) 209, 415, 517, 585, 1025, 1191, 1253, 1360, 1489
Hpy8I GTNNAC 2 cut(s) 389, 994
HpyAV CCTTC 7 cut(s) 328, 467, 661, 698, 737, 853, 899
HpyCH4III ACNGT 3 cut(s) 67, 1058, 1250
HpyCH4IV ACGT 2 cut(s) 1005, 1464
HpyCH4V TGCA 9 cut(s) 433, 750, 1088, 1352, 1356, 1382, 1417, 1505, 1592
HpyF10VI GCNNNNNNNGC 3 cut(s) 1110, 1330, 1388
HpyF3I CTNAG 6 cut(s) 27, 161, 196, 221, 525, 686
HpySE526I ACGT 2 cut(s) 1005, 1464
HspAI GCGC 3 cut(s) 363, 612, 1562
Kpn2I TCCGGA 2 cut(s) 208, 1024
Kzo9I GATC 6 cut(s) 140, 508, 597, 757, 1177, 1601
LmnI GCTCC 2 cut(s) 577, 1299
Lsp1109I GCAGC 2 cut(s) 734, 1336
LweI GCATC 2 cut(s) 734, 1365
MaeI CTAG 3 cut(s) 467, 489, 1293
MaeII ACGT 2 cut(s) 1005, 1464
MaeIII GTNAC 7 cut(s) 156, 917, 1061, 1188, 1277, 1460, 1508
MalI GATC 6 cut(s) 142, 510, 599, 759, 1179, 1603
MboI GATC 6 cut(s) 140, 508, 597, 757, 1177, 1601
MboII GAAGA 9 cut(s) 279, 309, 430, 576, 799, 983, 1013, 1547, 1583
MflI RGATCY 1 cut(s) 1177
MlsI TGGCCA 2 cut(s) 1324, 1520
MluCI AATT 8 cut(s) 75, 167, 396, 792, 1120, 1202, 1484, 1606
MluNI TGGCCA 2 cut(s) 1324, 1520
MlyI GAGTC 3 cut(s) 415, 1090, 1196
MmeI TCCRAC 2 cut(s) 746, 1512
Mox20I TGGCCA 2 cut(s) 1324, 1520
Mph1103I ATGCAT 1 cut(s) 1358
MroI TCCGGA 2 cut(s) 208, 1024
MroXI GAANNNNTTC 1 cut(s) 863
MscI TGGCCA 2 cut(s) 1324, 1520
MseI TTAA 7 cut(s) 402, 533, 818, 840, 936, 944, 1223
Msp20I TGGCCA 2 cut(s) 1324, 1520
MspA1I CMGCKG 1 cut(s) 1104
MspI CCGG 3 cut(s) 209, 575, 1025
Mva1269I GAATGC 1 cut(s) 665
MvnI CGCG 1 cut(s) 612
MwoI GCNNNNNNNGC 3 cut(s) 1110, 1330, 1388
NcoI CCATGG 1 cut(s) 1164
NdeII GATC 6 cut(s) 140, 508, 597, 757, 1177, 1601
NlaIV GGNNCC 1 cut(s) 1373
NmeAIII GCCGAG 1 cut(s) 1429
NmuCI GTSAC 3 cut(s) 156, 1188, 1277
NsiI ATGCAT 1 cut(s) 1358
NspI RCATGY 1 cut(s) 1356
PaeI GCATGC 1 cut(s) 1356
PaeR7I CTCGAG 1 cut(s) 1565
PagI TCATGA 1 cut(s) 1252
PctI GAATGC 1 cut(s) 665
PdmI GAANNNNTTC 1 cut(s) 863
PfeI GAWTC 6 cut(s) 53, 270, 459, 581, 1016, 1132
PflFI GACNNNGTC 3 cut(s) 353, 1031, 1495
PkrI GCNGC 3 cut(s) 678, 749, 1351
PleI GAGTC 3 cut(s) 415, 1089, 1195
PpsI GAGTC 3 cut(s) 415, 1089, 1195
PspFI CCCAGC 1 cut(s) 359
PspN4I GGNNCC 1 cut(s) 1373
PspPI GGNCC 3 cut(s) 1371, 1431, 1497
PspXI VCTCGAGB 1 cut(s) 1565
PstI CTGCAG 1 cut(s) 435
PsuI RGATCY 1 cut(s) 1177
PsyI GACNNNGTC 3 cut(s) 353, 1031, 1495
RsaI GTAC 1 cut(s) 501
RsaNI GTAC 1 cut(s) 500
SaqAI TTAA 7 cut(s) 402, 533, 818, 840, 936, 944, 1223
SatI GCNGC 3 cut(s) 677, 748, 1350
Sau3AI GATC 6 cut(s) 140, 508, 597, 757, 1177, 1601
Sau96I GGNCC 3 cut(s) 1371, 1431, 1497
ScaI AGTACT 1 cut(s) 501
SchI GAGTC 3 cut(s) 415, 1090, 1196
SfaNI GCATC 2 cut(s) 734, 1365
SfcI CTRYAG 3 cut(s) 259, 431, 558
Sfr274I CTCGAG 1 cut(s) 1565
SinI GGWCC 1 cut(s) 1497
SlaI CTCGAG 1 cut(s) 1565
SmlI CTYRAG 2 cut(s) 898, 1565
SmoI CTYRAG 2 cut(s) 898, 1565
SphI GCATGC 1 cut(s) 1356
Sse9I AATT 8 cut(s) 75, 167, 396, 792, 1120, 1202, 1484, 1606
SsiI CCGC 2 cut(s) 676, 1102
SspI AATATT 2 cut(s) 127, 245
SspMI CTAG 3 cut(s) 467, 489, 1293
StyI CCWWGG 2 cut(s) 1143, 1164
TaaI ACNGT 3 cut(s) 67, 1058, 1250
TaiI ACGT 2 cut(s) 1008, 1467
TaqI TCGA 5 cut(s) 202, 711, 958, 1566, 1604
TasI AATT 8 cut(s) 75, 167, 396, 792, 1120, 1202, 1484, 1606
TatI WGTACW 1 cut(s) 499
TauI GCSGC 1 cut(s) 679
TfiI GAWTC 6 cut(s) 53, 270, 459, 581, 1016, 1132
Tru1I TTAA 7 cut(s) 402, 533, 818, 840, 936, 944, 1223
Tru9I TTAA 7 cut(s) 402, 533, 818, 840, 936, 944, 1223
TscAI CASTG 5 cut(s) 15, 169, 301, 529, 1351
TseFI GTSAC 3 cut(s) 156, 1188, 1277
TseI GCWGC 2 cut(s) 747, 1349
Tsp45I GTSAC 3 cut(s) 156, 1188, 1277
TspGWI ACGGA 2 cut(s) 416, 756
TspRI CASTG 5 cut(s) 15, 169, 301, 529, 1351
Tth111I GACNNNGTC 3 cut(s) 353, 1031, 1495
VpaK11BI GGWCC 1 cut(s) 1497
XapI RAATTY 3 cut(s) 792, 1484, 1606
XceI RCATGY 1 cut(s) 1356
XcmI CCANNNNNNNNNTGG 3 cut(s) 1171, 1332, 1528
XhoI CTCGAG 1 cut(s) 1565
XmnI GAANNNNTTC 1 cut(s) 863
XspI CTAG 3 cut(s) 467, 489, 1293
ZrmI AGTACT 1 cut(s) 501
Zsp2I ATGCAT 1 cut(s) 1358
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.