MD05G1115200.v1.1

Alkaline neutral invertase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr05
OG0008953
Physical Location & Seq
Reverse (-)
23631622 .. 23636799
5178 bp
Loading structure...
UTR
Exon/CDS
Intron
MD05G1115200.v1.1.491

Sequence Viewer

Length: 1719 bp
ATGTCAGCGCCCAATGCGGATTTGTCCCACAATGGAGATATGAGACATGTTGATTCACTATGTAGTGTGGCAGAAATTGACGAAATTGATTTTTCAAAGTTACTAGACAAACCAAGCCTTCTGAATATGGAGAGAAAAAGATCATTTGATGAGAGGTCACTCAGTGAACTATCTGTTGCGGTGTCCCCACATCCCTCATCGAGAAATGCTGAGAACACTTCCAGATTTTTTGAACCTCCTGAGTACGTATTTTCGCCCTGTAGTAGAATGTCACTCATGGGTACCCCTAGGTCCCAGATGGGCTTTGAGCCACATCCAATGATTGGTGAAGCTTGGGCTACTTTGAGGCGTTCATTGGTGTTTTTCCGTGGTCAGCCAGTTGGGACAATTGCTGCCTCAGATACCACAGAAGAAAATCTTAACTATGACCAGGTGTTTGTTCGAGACTTCGTCCCCAGCGGATTGGCTTTTCTCATGAATGGTGAACCAGAAATAGTTAAAAATTTTATCTTGAAGACTCTTCGTCTTCAGTCGTGGGAGAAAAAGATTGATAGGTTTCATCTAGGAGAAGGAGTAATGCCAGCTAGTTTCAAAGTACTCCATGATCCTGTCCGGAATAGTGAGACTTTAATAGCAGATTTTGGTGAGAGTGCGATAGGAAGAGTTGCTCCTGTTGATTCTGGATTTTGGTGGATCATATTGCTTCGAGCATACACAAAATCCACTGGAGACTCTTCATTGGCTGAACTGCCTGAATGCCAGAAGGGTATGCGGCTTATTCTAAGTTTATGCCTATCGGAGGGGTTTGATACATTTCCAACCCTTCTTTGTGCTGATGGATGCTGCATGATTGATCGTAGAATGGGTGTTTACGGGTATCCCATCGAAATTCAGGCTCTCTTCTTCATGGCTTTAAGATGTGCTTTGCTTCTTCTTAAGCACGATGATGAGGGGAAGGAGTTTATTGAGCGCATATCAAAACGCCTTCATGCCTTGAGCTATCACATGAGAAGTTACTTTTGGCTAGACTTTAAGCAGTTAAATGACATATATCGTTATAAAACTGAAGAATATTCGCATACAGCAGTCAACAAGTTCAATGTGATGCCAGATTCTCTTCCGGACTGGGTTTTTGACTTTATGCCAACTCGTGGTGGTTACTTTATAGGGAATGTCAGTCCTGCCAGAATGGATTTCCGTTGGTTTTGCTTGGGTAATTGTGTAGCGATTCTTTCGTCCTTGGCAACCCCTGAACAATCTATGGCAATAATGGATCTAATAGAATCACGATGGGAGGAATTGGCAGGAGAAATGCCACTAAAGGTCTGTTATCCAGCAATAGAAAATCATGAATGGAAGATTGTAACAGGCTGCGACCCGAAAAATACTAGATGGAGCTACCACAATGGGGGATCTTGGCCAGTGCTCTTATGGCTTCTCACTGCTGCATCCATCAAGACTGGGAGGCCCCAGATAGCTAGACGCGCCATTGAACTTGCCGAGAGCAGGTTGCTGAAAGACAACTGGCCTGAGTACTACGATGGGAAACTTGGTCGTTATGTTGGGAAGCAAGCCCGTAAATTCCAGACCTGGTCAGTTGCGGGGTACTTGGTAGCGAAGATGATGTTGGAAGACCCATCTCATTTGGGGATGATAGCGCTCGAGGATGACAAACAGATGAAGCCAGCAATGAGGAGATCAAATTCGTGGACTTGCTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005829 GO:0044424 GO:0044444 GO:0044464
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Glyco_hydro_100

Protein Analysis

573

Amino Acids

65.31

Weight (kDa)

6.06

Isoelectric Point (pI)

55.29

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Glyco_hydro_100 PF12899 111 - 546 8.7e-219 Alkaline and neutral invertase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 1059
Acc36I ACCTGC 1 cut(s) 1497
Acc65I GGTACC 1 cut(s) 281
AccB1I GGYRCC 1 cut(s) 281
AccB7I CCANNNNNTGG 2 cut(s) 323, 1151
AccII CGCG 1 cut(s) 1485
AccIII TCCGGA 2 cut(s) 612, 1120
AciI CCGC 5 cut(s) 17, 179, 459, 772, 1601
AclWI GGATC 4 cut(s) 599, 701, 1281, 1420
AcoI YGGCCR 1 cut(s) 1418
AcsI RAATTY 4 cut(s) 502, 888, 1580, 1702
AcuI CTGAAG 2 cut(s) 512, 1086
AdeI CACNNNGTG 1 cut(s) 164
AfaI GTAC 5 cut(s) 245, 283, 597, 1535, 1607
AfeI AGCGCT 1 cut(s) 1659
AfiI CCNNNNNNNGG 5 cut(s) 323, 799, 1151, 1408, 1506
AflII CTTAAG 1 cut(s) 935
AflIII ACRYGT 1 cut(s) 46
AgsI TTSAA 6 cut(s) 96, 233, 514, 592, 1099, 1493
AhdI GACNNNNNGTC 1 cut(s) 522
AjnI CCWGG 2 cut(s) 429, 1589
AjuI GAANNNNNNNTTGG 4 cut(s) 1003, 1035, 1610, 1642
AluBI AGCT 5 cut(s) 332, 584, 999, 1398, 1478
AluI AGCT 5 cut(s) 332, 584, 999, 1398, 1478
Alw21I GWGCWC 1 cut(s) 1428
Alw26I GTCTC 4 cut(s) 37, 438, 617, 723
AlwI GGATC 4 cut(s) 599, 701, 1281, 1420
Ama87I CYCGRG 1 cut(s) 1661
Aor13HI TCCGGA 2 cut(s) 612, 1120
Aor51HI AGCGCT 1 cut(s) 1659
AoxI GGCC 3 cut(s) 1418, 1466, 1526
ApeKI GCWGC 4 cut(s) 392, 843, 1371, 1445
ApoI RAATTY 4 cut(s) 502, 888, 1580, 1702
ArsI GACNNNNNNTTYG 4 cut(s) 419, 451, 1115, 1147
Asp718I GGTACC 1 cut(s) 281
AspA2I CCTAGG 1 cut(s) 287
AspLEI GCGC 4 cut(s) 10, 972, 1487, 1660
AspS9I GGNCC 2 cut(s) 291, 1467
AsuHPI GGTGA 3 cut(s) 338, 494, 656
AvaI CYCGRG 1 cut(s) 1661
AvaII GGWCC 1 cut(s) 291
AvrII CCTAGG 1 cut(s) 287
BalI TGGCCA 1 cut(s) 1420
BanI GGYRCC 1 cut(s) 281
BauI CACGAG 1 cut(s) 1149
BbsI GAAGAC 3 cut(s) 518, 521, 1638
Bbv12I GWGCWC 1 cut(s) 1428
BbvI GCAGC 4 cut(s) 379, 830, 1358, 1432
BccI CCATC 8 cut(s) 292, 830, 890, 1284, 1386, 1460, 1535, 1645
BciT130I CCWGG 2 cut(s) 431, 1591
BciVI GTATCC 1 cut(s) 888
BcoDI GTCTC 4 cut(s) 37, 438, 617, 723
BfaI CTAG 7 cut(s) 104, 288, 563, 585, 1025, 1389, 1479
BfmI CTRYAG 1 cut(s) 259
BfoI RGCGCY 2 cut(s) 11, 1661
BfrI CTTAAG 1 cut(s) 935
BfuAI ACCTGC 1 cut(s) 1497
BfuI GTATCC 1 cut(s) 888
BisI GCNGC 5 cut(s) 393, 773, 844, 1372, 1446
BlnI CCTAGG 1 cut(s) 287
BlsI GCNGC 5 cut(s) 394, 774, 845, 1373, 1447
BmcAI AGTACT 2 cut(s) 597, 1535
Bme1390I CCNGG 2 cut(s) 431, 1591
Bme18I GGWCC 1 cut(s) 291
BmeRI GACNNNNNGTC 1 cut(s) 522
BmeT110I CYCGRG 1 cut(s) 1661
BmgT120I GGNCC 2 cut(s) 291, 1467
BmiI GGNNCC 3 cut(s) 283, 293, 1469
BmrFI CCNGG 2 cut(s) 431, 1591
BmrI ACTGGG 2 cut(s) 1135, 1470
BmsI GCATC 3 cut(s) 830, 1095, 1457
BmuI ACTGGG 2 cut(s) 1135, 1470
BpiI GAAGAC 3 cut(s) 518, 521, 1638
BpmI CTGGAG 1 cut(s) 747
BpuEI CTTGAG 1 cut(s) 1015
BsaAI YACGTR 1 cut(s) 247
BsaJI CCNNGG 3 cut(s) 287, 367, 1239
BsaWI WCCGGW 2 cut(s) 612, 1120
BsaXI ACNNNNNCTCC 2 cut(s) 558, 588
Bsc4I CCNNNNNNNGG 5 cut(s) 323, 799, 1151, 1408, 1506
Bse1I ACTGG 6 cut(s) 377, 730, 1130, 1421, 1465, 1529
Bse3DI GCAATG 1 cut(s) 1695
BseAI TCCGGA 2 cut(s) 612, 1120
BseBI CCWGG 2 cut(s) 431, 1591
BseDI CCNNGG 3 cut(s) 287, 367, 1239
BseGI GGATG 6 cut(s) 190, 313, 845, 1448, 1656, 1672
BseLI CCNNNNNNNGG 5 cut(s) 323, 799, 1151, 1408, 1506
BseMI GCAATG 1 cut(s) 1695
BseMII CTCAG 5 cut(s) 175, 201, 231, 411, 1521
BseNI ACTGG 6 cut(s) 377, 730, 1130, 1421, 1465, 1529
BseRI GAGGAG 1 cut(s) 1708
BseXI GCAGC 4 cut(s) 379, 830, 1358, 1432
BseYI CCCAGC 1 cut(s) 455
Bsh1236I CGCG 1 cut(s) 1485
BshFI GGCC 3 cut(s) 1420, 1468, 1528
BshNI GGYRCC 1 cut(s) 281
BsiHKAI GWGCWC 1 cut(s) 1428
BsiHKCI CYCGRG 1 cut(s) 1661
BsiSI CCGG 2 cut(s) 613, 1121
BslFI GGGAC 5 cut(s) 10, 169, 277, 397, 437
BslI CCNNNNNNNGG 5 cut(s) 323, 799, 1151, 1408, 1506
BsmAI GTCTC 4 cut(s) 37, 438, 617, 723
BsmFI GGGAC 5 cut(s) 10, 169, 277, 397, 437
BsmI GAATGC 1 cut(s) 761
BsnI GGCC 3 cut(s) 1420, 1468, 1528
BsoBI CYCGRG 1 cut(s) 1661
Bsp1286I GDGCHC 1 cut(s) 1428
Bsp13I TCCGGA 2 cut(s) 612, 1120
Bsp143I GATC 7 cut(s) 140, 604, 693, 853, 1273, 1412, 1697
BspACI CCGC 5 cut(s) 17, 179, 459, 772, 1601
BspANI GGCC 3 cut(s) 1420, 1468, 1528
BspCNI CTCAG 5 cut(s) 174, 202, 232, 410, 1522
BspEI TCCGGA 2 cut(s) 612, 1120
BspFNI CGCG 1 cut(s) 1485
BspHI TCATGA 2 cut(s) 474, 1348
BspLI GGNNCC 3 cut(s) 283, 293, 1469
BspMI ACCTGC 1 cut(s) 1497
BspPI GGATC 4 cut(s) 599, 701, 1281, 1420
BspT107I GGYRCC 1 cut(s) 281
BspTI CTTAAG 1 cut(s) 935
BsrDI GCAATG 1 cut(s) 1695
BsrI ACTGG 6 cut(s) 377, 730, 1130, 1421, 1465, 1529
BssECI CCNNGG 3 cut(s) 287, 367, 1239
BssMI GATC 7 cut(s) 140, 604, 693, 853, 1273, 1412, 1697
BssSI CACGAG 1 cut(s) 1149
BssT1I CCWWGG 2 cut(s) 287, 1239
Bst2BI CACGAG 1 cut(s) 1149
Bst2UI CCWGG 2 cut(s) 431, 1591
Bst6I CTCTTC 5 cut(s) 525, 655, 739, 905, 1122
BstAFI CTTAAG 1 cut(s) 935
BstBAI YACGTR 1 cut(s) 247
BstC8I GCNNGC 3 cut(s) 582, 1572, 1686
BstDEI CTNAG 6 cut(s) 161, 210, 240, 397, 782, 1530
BstDSI CCRYGG 1 cut(s) 367
BstENI CCTNNNNNAGG 1 cut(s) 797
BstF5I GGATG 6 cut(s) 190, 313, 845, 1448, 1656, 1672
BstFNI CGCG 1 cut(s) 1485
BstH2I RGCGCY 2 cut(s) 11, 1661
BstHHI GCGC 4 cut(s) 10, 972, 1487, 1660
BstKTI GATC 7 cut(s) 143, 607, 696, 856, 1276, 1415, 1700
BstMAI GTCTC 4 cut(s) 37, 438, 617, 723
BstMBI GATC 7 cut(s) 140, 604, 693, 853, 1273, 1412, 1697
BstMWI GCNNNNNNNGC 3 cut(s) 14, 1432, 1484
BstNI CCWGG 2 cut(s) 431, 1591
BstNSI RCATGY 1 cut(s) 50
BstSCI CCNGG 2 cut(s) 429, 1589
BstSFI CTRYAG 1 cut(s) 259
BstSNI TACGTA 1 cut(s) 247
BstUI CGCG 1 cut(s) 1485
BstV1I GCAGC 4 cut(s) 379, 830, 1358, 1432
BstV2I GAAGAC 3 cut(s) 518, 521, 1638
BstX2I RGATCY 2 cut(s) 1273, 1412
BstXI CCANNNNNNTGG 1 cut(s) 463
BstYI RGATCY 2 cut(s) 1273, 1412
BsuI GTATCC 1 cut(s) 888
BsuRI GGCC 3 cut(s) 1420, 1468, 1528
BtgI CCRYGG 1 cut(s) 367
BtsCI GGATG 6 cut(s) 190, 313, 845, 1448, 1656, 1672
BtsI GCAGTG 1 cut(s) 1440
BtsIMutI CAGTG 4 cut(s) 169, 723, 1428, 1440
BveI ACCTGC 1 cut(s) 1497
Cac8I GCNNGC 3 cut(s) 582, 1572, 1686
CciI TCATGA 2 cut(s) 474, 1348
CfoI GCGC 4 cut(s) 10, 972, 1487, 1660
Cfr13I GGNCC 2 cut(s) 291, 1467
CseI GACGC 1 cut(s) 1491
CsiI ACCWGGT 2 cut(s) 429, 1589
Csp6I GTAC 5 cut(s) 244, 282, 596, 1534, 1606
CviAII CATG 9 cut(s) 47, 277, 475, 602, 847, 907, 989, 1006, 1349
CviQI GTAC 5 cut(s) 244, 282, 596, 1534, 1606
DdeI CTNAG 6 cut(s) 161, 210, 240, 397, 782, 1530
DpnI GATC 7 cut(s) 142, 606, 695, 855, 1275, 1414, 1699
DpnII GATC 7 cut(s) 140, 604, 693, 853, 1273, 1412, 1697
DraIII CACNNNGTG 1 cut(s) 164
DriI GACNNNNNGTC 1 cut(s) 522
EaeI YGGCCR 1 cut(s) 1418
Eam1104I CTCTTC 5 cut(s) 525, 655, 739, 905, 1122
Eam1105I GACNNNNNGTC 1 cut(s) 522
EarI CTCTTC 5 cut(s) 525, 655, 739, 905, 1122
Eco105I TACGTA 1 cut(s) 247
Eco130I CCWWGG 2 cut(s) 287, 1239
Eco47I GGWCC 1 cut(s) 291
Eco47III AGCGCT 1 cut(s) 1659
Eco57I CTGAAG 2 cut(s) 512, 1086
Eco88I CYCGRG 1 cut(s) 1661
EcoNI CCTNNNNNAGG 1 cut(s) 797
EcoO109I RGGNCCY 2 cut(s) 291, 1467
EcoRII CCWGG 2 cut(s) 429, 1589
EcoT14I CCWWGG 2 cut(s) 287, 1239
ErhI CCWWGG 2 cut(s) 287, 1239
FaeI CATG 9 cut(s) 50, 280, 478, 605, 850, 910, 992, 1009, 1352
FalI AAGNNNNNCTT 4 cut(s) 402, 434, 907, 939
FaqI GGGAC 5 cut(s) 10, 169, 277, 397, 437
FatI CATG 9 cut(s) 46, 276, 474, 601, 846, 906, 988, 1005, 1348
FauI CCCGC 1 cut(s) 1594
Fnu4HI GCNGC 5 cut(s) 393, 773, 844, 1372, 1446
FokI GGATG 6 cut(s) 177, 300, 852, 1435, 1663, 1679
Fsp4HI GCNGC 5 cut(s) 393, 773, 844, 1372, 1446
FspBI CTAG 7 cut(s) 104, 288, 563, 585, 1025, 1389, 1479
GlaI GCGC 4 cut(s) 9, 971, 1486, 1659
GluI GCNGC 5 cut(s) 393, 773, 844, 1372, 1446
GsaI CCCAGC 1 cut(s) 459
GsuI CTGGAG 1 cut(s) 747
HaeII RGCGCY 2 cut(s) 11, 1661
HaeIII GGCC 3 cut(s) 1420, 1468, 1528
HapII CCGG 2 cut(s) 613, 1121
HgaI GACGC 1 cut(s) 1491
HhaI GCGC 4 cut(s) 10, 972, 1487, 1660
Hin1II CATG 9 cut(s) 50, 280, 478, 605, 850, 910, 992, 1009, 1352
Hin6I GCGC 4 cut(s) 8, 970, 1485, 1658
HinP1I GCGC 4 cut(s) 8, 970, 1485, 1658
HincII GTYRAC 1 cut(s) 1090
HindII GTYRAC 1 cut(s) 1090
HindIII AAGCTT 1 cut(s) 330
HinfI GANTC 7 cut(s) 53, 517, 677, 731, 1112, 1228, 1283
HpaII CCGG 2 cut(s) 613, 1121
HphI GGTGA 3 cut(s) 338, 494, 656
Hpy166II GTNNAC 5 cut(s) 167, 485, 871, 1090, 1710
Hpy188I TCNGA 3 cut(s) 123, 400, 799
Hpy8I GTNNAC 5 cut(s) 167, 485, 871, 1090, 1710
HpyAV CCTTC 6 cut(s) 128, 563, 757, 833, 949, 995
HpyCH4IV ACGT 1 cut(s) 246
HpyCH4V TGCA 2 cut(s) 846, 1448
HpyF10VI GCNNNNNNNGC 3 cut(s) 14, 1432, 1484
HpyF3I CTNAG 6 cut(s) 161, 210, 240, 397, 782, 1530
HpySE526I ACGT 1 cut(s) 246
Hsp92II CATG 9 cut(s) 50, 280, 478, 605, 850, 910, 992, 1009, 1352
HspAI GCGC 4 cut(s) 8, 970, 1485, 1658
Kpn2I TCCGGA 2 cut(s) 612, 1120
KpnI GGTACC 1 cut(s) 285
Kzo9I GATC 7 cut(s) 140, 604, 693, 853, 1273, 1412, 1697
LmnI GCTCC 2 cut(s) 673, 1395
Lsp1109I GCAGC 4 cut(s) 379, 830, 1358, 1432
LweI GCATC 3 cut(s) 830, 1095, 1457
MabI ACCWGGT 2 cut(s) 429, 1589
MaeI CTAG 7 cut(s) 104, 288, 563, 585, 1025, 1389, 1479
MaeII ACGT 1 cut(s) 246
MaeIII GTNAC 6 cut(s) 99, 156, 270, 1013, 1157, 1363
MalI GATC 7 cut(s) 142, 606, 695, 855, 1275, 1414, 1699
MboI GATC 7 cut(s) 140, 604, 693, 853, 1273, 1412, 1697
MfeI CAATTG 1 cut(s) 387
MflI RGATCY 2 cut(s) 1273, 1412
MhlI GDGCHC 1 cut(s) 1428
MlsI TGGCCA 1 cut(s) 1420
MluCI AATT 9 cut(s) 75, 84, 387, 502, 888, 1216, 1298, 1580, 1702
MluNI TGGCCA 1 cut(s) 1420
MlyI GAGTC 2 cut(s) 511, 725
MmeI TCCRAC 2 cut(s) 842, 1608
Mox20I TGGCCA 1 cut(s) 1420
MroI TCCGGA 2 cut(s) 612, 1120
MscI TGGCCA 1 cut(s) 1420
MseI TTAA 7 cut(s) 420, 498, 629, 914, 936, 1032, 1040
MslI CAYNNNNRTG 1 cut(s) 945
Msp20I TGGCCA 1 cut(s) 1420
MspA1I CMGCKG 1 cut(s) 459
MspCI CTTAAG 1 cut(s) 935
MspI CCGG 2 cut(s) 613, 1121
MspR9I CCNGG 2 cut(s) 431, 1591
MunI CAATTG 1 cut(s) 387
Mva1269I GAATGC 1 cut(s) 761
MvaI CCWGG 2 cut(s) 431, 1591
MvnI CGCG 1 cut(s) 1485
MwoI GCNNNNNNNGC 3 cut(s) 14, 1432, 1484
NdeII GATC 7 cut(s) 140, 604, 693, 853, 1273, 1412, 1697
NlaIII CATG 9 cut(s) 50, 280, 478, 605, 850, 910, 992, 1009, 1352
NlaIV GGNNCC 3 cut(s) 283, 293, 1469
NmeAIII GCCGAG 1 cut(s) 1525
NmuCI GTSAC 2 cut(s) 156, 270
NspI RCATGY 1 cut(s) 50
PaeR7I CTCGAG 1 cut(s) 1661
PagI TCATGA 2 cut(s) 474, 1348
PciI ACATGT 1 cut(s) 46
PctI GAATGC 1 cut(s) 761
PfeI GAWTC 5 cut(s) 53, 677, 1112, 1228, 1283
PflFI GACNNNGTC 2 cut(s) 449, 1591
PflMI CCANNNNNTGG 2 cut(s) 323, 1151
PkrI GCNGC 5 cut(s) 394, 774, 845, 1373, 1447
PleI GAGTC 2 cut(s) 511, 725
PpsI GAGTC 2 cut(s) 511, 725
Ppu21I YACGTR 1 cut(s) 247
PpuMI RGGWCCY 1 cut(s) 291
PscI ACATGT 1 cut(s) 46
PsiI TTATAA 1 cut(s) 1059
Psp5II RGGWCCY 1 cut(s) 291
Psp6I CCWGG 2 cut(s) 429, 1589
PspFI CCCAGC 1 cut(s) 455
PspGI CCWGG 2 cut(s) 429, 1589
PspN4I GGNNCC 3 cut(s) 283, 293, 1469
PspPI GGNCC 2 cut(s) 291, 1467
PspPPI RGGWCCY 1 cut(s) 291
PspXI VCTCGAGB 1 cut(s) 1661
PsuI RGATCY 2 cut(s) 1273, 1412
PsyI GACNNNGTC 2 cut(s) 449, 1591
RsaI GTAC 5 cut(s) 245, 283, 597, 1535, 1607
RsaNI GTAC 5 cut(s) 244, 282, 596, 1534, 1606
RseI CAYNNNNRTG 1 cut(s) 945
SaqAI TTAA 7 cut(s) 420, 498, 629, 914, 936, 1032, 1040
SatI GCNGC 5 cut(s) 393, 773, 844, 1372, 1446
Sau3AI GATC 7 cut(s) 140, 604, 693, 853, 1273, 1412, 1697
Sau96I GGNCC 2 cut(s) 291, 1467
ScaI AGTACT 2 cut(s) 597, 1535
SchI GAGTC 2 cut(s) 511, 725
ScrFI CCNGG 2 cut(s) 431, 1591
SduI GDGCHC 1 cut(s) 1428
SexAI ACCWGGT 2 cut(s) 429, 1589
SfaNI GCATC 3 cut(s) 830, 1095, 1457
SfcI CTRYAG 1 cut(s) 259
Sfr274I CTCGAG 1 cut(s) 1661
SinI GGWCC 1 cut(s) 291
SlaI CTCGAG 1 cut(s) 1661
SmiMI CAYNNNNRTG 1 cut(s) 945
SmlI CTYRAG 3 cut(s) 935, 994, 1661
SmoI CTYRAG 3 cut(s) 935, 994, 1661
SnaBI TACGTA 1 cut(s) 247
Sse9I AATT 9 cut(s) 75, 84, 387, 502, 888, 1216, 1298, 1580, 1702
SsiI CCGC 5 cut(s) 17, 179, 459, 772, 1601
SspI AATATT 1 cut(s) 1073
SspMI CTAG 7 cut(s) 104, 288, 563, 585, 1025, 1389, 1479
StyD4I CCNGG 2 cut(s) 429, 1589
StyI CCWWGG 2 cut(s) 287, 1239
TaiI ACGT 1 cut(s) 249
TaqI TCGA 5 cut(s) 200, 442, 706, 885, 1662
TasI AATT 9 cut(s) 75, 84, 387, 502, 888, 1216, 1298, 1580, 1702
TatI WGTACW 2 cut(s) 595, 1533
TauI GCSGC 1 cut(s) 775
TfiI GAWTC 5 cut(s) 53, 677, 1112, 1228, 1283
Tru1I TTAA 7 cut(s) 420, 498, 629, 914, 936, 1032, 1040
Tru9I TTAA 7 cut(s) 420, 498, 629, 914, 936, 1032, 1040
TscAI CASTG 4 cut(s) 169, 730, 1428, 1447
TseFI GTSAC 2 cut(s) 156, 270
TseI GCWGC 4 cut(s) 392, 843, 1371, 1445
Tsp45I GTSAC 2 cut(s) 156, 270
TspDTI ATGAA 8 cut(s) 342, 491, 548, 726, 895, 977, 1365, 1694
TspGWI ACGGA 2 cut(s) 356, 1187
TspRI CASTG 4 cut(s) 169, 730, 1428, 1447
Tth111I GACNNNGTC 2 cut(s) 449, 1591
Van91I CCANNNNNTGG 2 cut(s) 323, 1151
Vha464I CTTAAG 1 cut(s) 935
VpaK11BI GGWCC 1 cut(s) 291
XagI CCTNNNNNAGG 1 cut(s) 797
XapI RAATTY 4 cut(s) 502, 888, 1580, 1702
XceI RCATGY 1 cut(s) 50
XcmI CCANNNNNNNNNTGG 1 cut(s) 1428
XhoI CTCGAG 1 cut(s) 1661
XmaJI CCTAGG 1 cut(s) 287
XspI CTAG 7 cut(s) 104, 288, 563, 585, 1025, 1389, 1479
ZrmI AGTACT 2 cut(s) 597, 1535
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.