Rh6DG025800

Alkaline neutral invertase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr6D
OG0008953
Physical Location & Seq
Forward (+)
2247698 .. 2251573
3876 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh6DG025800.1

Sequence Viewer

Length: 1722 bp
ATGTCATCGCTGAATGGGGATGTGTCTCAGAATGGAAATACAAGAAATATGGATTCCCTATTTACTGTGGCAGAAATTGAGGAAGTTGATTTCTCAAAGCTATTAGACAGACCTAAACCTCTGAATATTGAGAGACAACGATCATTTGATGAGAGGTCACTCAGTGAATTATCTGTGGGGTTTTCTCCACGCCTCTCAGCTCGACATCCGGAGAACTCTCTTAGGAACTATGACCCACATGAATATTTATTTTCCCCCTGTAGTAGAAGGTCAGTCATTAGTTGTACTCCTAGATCACAGGCTGGCTTTGAGCCACATCCGATGGTTGCTGAAGCTTGGGAGAATTTGAGGCGTTCATTGGTTTTTTTCCGCGGTCAGCCGGTTGGGACAATTGCTGCAACAGATACATCTGATGAAAAACTCAACTATGATCAGGTGTTTGTGAGAGACTTTGTCCCCAGTGCATTGGCTTTTCTGATGAATGGTGAACCTGAAATTGTTAAAAACTTTATCTTGAAGACTCTCCGTCTGCAGTCATGGGAGAAAAAGATTGATAGATTCCATCTAGGAGAAGGAGTAATGCCAGCTAGTTTCAAAGTACTCCATGATCCAGTCAGGAACACTGAGACTTTAATGGCAGATTTTGGTGAGAGTGCTATAGGAAGAGTTGCTCCGGTTGATTCTGGATTTTGGTGGATCATATTGCTTCGCGCATACACAAAATCTACTGGAGACACATCATTGGCTGAACGGCCTGAATGCCAGAAGGGTATGCGGCTTATTCTAAGTTTATGTCTTTCGGAAGGGTTCGACACATTTCCAACCCTTCTTTGTGCTGACGGATGCTGCATGATTGATCGTAGAATGGGTGTTTATGGATATCCCATTGAAATTCAGGCTCTTTTCTTCATGGCTTTAAGATGTGCTTTGCTTTTACTTAACAAAGATGATGAGGGGAAGGAGTTCATTGAGCGAATAGTAAAACGCCTTCATGCCTTGAGCTATCACATGAGAAGTTACTTTTGGCTTGACTTTAAGCAGTTAAATGATATATATCGATATAAAACTGAAGAATACTCACATACAGCCGTCAACAAGTTCAATGTGATACCAGATTCTCTTCCGGACTGGGTCTTTGATTTTATGCCAACACACGGTGGTTACTTTATTGGGAATGTGAGTCCTGCAAGAATGGATTTCCGCTGGTTTTGCTTGGGTAATTGTATAGCGATTCTTTCATCCTTGGCAACCCCTGAACAATCCATGGCAATCATGGATCTCATAGAGTCACGATGGGAGGAATTGGCAGGAGAAATGCCATTAAAGGTTTGTTATCCAGCCATAGACAGTCATGAATGGAGGATTGAAACAGGAAGTGACCCAAAAAATACTAGATGGAGCTACCACAATGGAGGTTCTTGGCCAGTTCTGCTATGGCTTCTCACTGCTGCATGCATCAAGACTGGACGGCCCCAGATTGCAAGACGAGCTATTGAACTTGCCGAGAGCAGGTTGCAGAAAGACAACTGGCCCGAATACTATGATGGAAAATGTGGTCGTTACGTAGGGAAGCAGGCTCGCAAATTCCAGACTTGGTCCATTGCAGGTTACTTGGTGGCCAAGATGATGTTGGAAGACCCATCCCATTTGGGAATGATAGCGCTCGAGGAAGACAAACAGATGAAGCCTGCAATGAGGAGATCGAATTCATGGACTTGCTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005829 GO:0044424 GO:0044444 GO:0044464
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Glyco_hydro_100

Protein Analysis

573

Amino Acids

65.77

Weight (kDa)

6.23

Isoelectric Point (pI)

59.04

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Glyco_hydro_100 PF12899 112 - 547 3.8e-220 Alkaline and neutral invertase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 2 cut(s) 1500, 1595
AccII CGCG 2 cut(s) 372, 711
AccIII TCCGGA 2 cut(s) 208, 1123
AciI CCGC 4 cut(s) 370, 372, 775, 1201
AclWI GGATC 3 cut(s) 602, 704, 1284
AcoI YGGCCR 2 cut(s) 1421, 1617
AcsI RAATTY 4 cut(s) 343, 891, 1583, 1705
AcuI CTGAAG 2 cut(s) 351, 1089
AdeI CACNNNGTG 2 cut(s) 164, 1157
AfaI GTAC 2 cut(s) 286, 600
AfeI AGCGCT 1 cut(s) 1662
AfiI CCNNNNNNNGG 2 cut(s) 1154, 1509
AgsI TTSAA 6 cut(s) 517, 595, 890, 1102, 1367, 1496
AhdI GACNNNNNGTC 1 cut(s) 525
AjuI GAANNNNNNNTTGG 2 cut(s) 1006, 1038
AluBI AGCT 7 cut(s) 100, 200, 335, 587, 1002, 1401, 1490
AluI AGCT 7 cut(s) 100, 200, 335, 587, 1002, 1401, 1490
Alw26I GTCTC 5 cut(s) 30, 127, 441, 620, 726
AlwI GGATC 3 cut(s) 602, 704, 1284
Ama87I CYCGRG 1 cut(s) 1664
Aor13HI TCCGGA 2 cut(s) 208, 1123
Aor51HI AGCGCT 1 cut(s) 1662
AoxI GGCC 5 cut(s) 752, 1421, 1469, 1529, 1617
ApeKI GCWGC 3 cut(s) 395, 846, 1448
ApoI RAATTY 4 cut(s) 343, 891, 1583, 1705
ArsI GACNNNNNNTTYG 2 cut(s) 1118, 1150
Asp700I GAANNNNTTC 1 cut(s) 962
AspLEI GCGC 2 cut(s) 713, 1663
AspS9I GGNCC 3 cut(s) 1470, 1530, 1596
AsuHPI GGTGA 2 cut(s) 497, 659
AvaI CYCGRG 1 cut(s) 1664
AvaII GGWCC 1 cut(s) 1596
BalI TGGCCA 2 cut(s) 1423, 1619
BarI GAAGNNNNNNTAC 2 cut(s) 972, 1004
BbsI GAAGAC 3 cut(s) 524, 1641, 1677
BbvI GCAGC 3 cut(s) 382, 833, 1435
BccI CCATC 6 cut(s) 316, 570, 1287, 1389, 1538, 1648
BceAI ACGGC 3 cut(s) 767, 1073, 1484
BclI TGATCA 1 cut(s) 430
BcoDI GTCTC 5 cut(s) 30, 127, 441, 620, 726
BfaI CTAG 4 cut(s) 291, 566, 588, 1392
BfmI CTRYAG 3 cut(s) 259, 530, 657
BfoI RGCGCY 1 cut(s) 1664
BfuAI ACCTGC 2 cut(s) 1500, 1595
BisI GCNGC 4 cut(s) 396, 776, 847, 1449
BlsI GCNGC 4 cut(s) 397, 777, 848, 1450
BmcAI AGTACT 1 cut(s) 600
Bme18I GGWCC 1 cut(s) 1596
BmeRI GACNNNNNGTC 1 cut(s) 525
BmeT110I CYCGRG 1 cut(s) 1664
BmgT120I GGNCC 3 cut(s) 1470, 1530, 1596
BmiI GGNNCC 1 cut(s) 1472
BmrI ACTGGG 2 cut(s) 453, 1138
BmsI GCATC 2 cut(s) 833, 1464
BmuI ACTGGG 2 cut(s) 453, 1138
BpiI GAAGAC 3 cut(s) 524, 1641, 1677
BpmI CTGGAG 1 cut(s) 750
BpuEI CTTGAG 1 cut(s) 1018
Bsa29I ATCGAT 1 cut(s) 1057
BsaAI YACGTR 1 cut(s) 1564
BsaBI GATNNNNATC 1 cut(s) 1053
BsaJI CCNNGG 3 cut(s) 370, 1242, 1263
BsaWI WCCGGW 3 cut(s) 208, 673, 1123
BsaXI ACNNNNNCTCC 2 cut(s) 561, 591
Bsc4I CCNNNNNNNGG 2 cut(s) 1154, 1509
Bse118I RCCGGY 1 cut(s) 379
Bse1I ACTGG 7 cut(s) 459, 611, 733, 1133, 1424, 1468, 1532
Bse3DI GCAATG 2 cut(s) 1599, 1698
Bse8I GATNNNNATC 1 cut(s) 1053
BseAI TCCGGA 2 cut(s) 208, 1123
BseCI ATCGAT 1 cut(s) 1057
BseDI CCNNGG 3 cut(s) 370, 1242, 1263
BseGI GGATG 6 cut(s) 25, 205, 316, 848, 1238, 1640
BseJI GATNNNNATC 1 cut(s) 1053
BseLI CCNNNNNNNGG 2 cut(s) 1154, 1509
BseMI GCAATG 2 cut(s) 1599, 1698
BseMII CTCAG 4 cut(s) 41, 175, 210, 615
BseNI ACTGG 7 cut(s) 459, 611, 733, 1133, 1424, 1468, 1532
BseRI GAGGAG 1 cut(s) 1711
BseXI GCAGC 3 cut(s) 382, 833, 1435
Bsh1236I CGCG 2 cut(s) 372, 711
BshFI GGCC 5 cut(s) 754, 1423, 1471, 1531, 1619
BshVI ATCGAT 1 cut(s) 1057
BsiHKCI CYCGRG 1 cut(s) 1664
BsiSI CCGG 4 cut(s) 209, 380, 674, 1124
BslFI GGGAC 2 cut(s) 400, 440
BslI CCNNNNNNNGG 2 cut(s) 1154, 1509
BsmAI GTCTC 5 cut(s) 30, 127, 441, 620, 726
BsmFI GGGAC 2 cut(s) 400, 440
BsmI GAATGC 1 cut(s) 764
BsnI GGCC 5 cut(s) 754, 1423, 1471, 1531, 1619
BsoBI CYCGRG 1 cut(s) 1664
Bsp13I TCCGGA 2 cut(s) 208, 1123
Bsp143I GATC 8 cut(s) 140, 293, 430, 607, 696, 856, 1276, 1700
Bsp19I CCATGG 1 cut(s) 1263
BspACI CCGC 4 cut(s) 370, 372, 775, 1201
BspANI GGCC 5 cut(s) 754, 1423, 1471, 1531, 1619
BspCNI CTCAG 4 cut(s) 40, 174, 209, 616
BspDI ATCGAT 1 cut(s) 1057
BspEI TCCGGA 2 cut(s) 208, 1123
BspFNI CGCG 2 cut(s) 372, 711
BspHI TCATGA 1 cut(s) 1351
BspLI GGNNCC 1 cut(s) 1472
BspMAI CTGCAG 1 cut(s) 534
BspMI ACCTGC 2 cut(s) 1500, 1595
BspPI GGATC 3 cut(s) 602, 704, 1284
BsrDI GCAATG 2 cut(s) 1599, 1698
BsrFI RCCGGY 1 cut(s) 379
BsrI ACTGG 7 cut(s) 459, 611, 733, 1133, 1424, 1468, 1532
BssAI RCCGGY 1 cut(s) 379
BssECI CCNNGG 3 cut(s) 370, 1242, 1263
BssMI GATC 8 cut(s) 140, 293, 430, 607, 696, 856, 1276, 1700
BssT1I CCWWGG 2 cut(s) 1242, 1263
Bst4CI ACNGT 3 cut(s) 67, 1157, 1349
Bst6I CTCTTC 2 cut(s) 658, 1125
BstBAI YACGTR 1 cut(s) 1564
BstC8I GCNNGC 6 cut(s) 304, 585, 1453, 1575, 1579, 1689
BstDEI CTNAG 6 cut(s) 27, 161, 196, 221, 624, 785
BstDSI CCRYGG 2 cut(s) 370, 1263
BstF5I GGATG 6 cut(s) 25, 205, 316, 848, 1238, 1640
BstFNI CGCG 2 cut(s) 372, 711
BstH2I RGCGCY 1 cut(s) 1664
BstHHI GCGC 2 cut(s) 713, 1663
BstKTI GATC 8 cut(s) 143, 296, 433, 610, 699, 859, 1279, 1703
BstMAI GTCTC 5 cut(s) 30, 127, 441, 620, 726
BstMBI GATC 8 cut(s) 140, 293, 430, 607, 696, 856, 1276, 1700
BstMWI GCNNNNNNNGC 3 cut(s) 1209, 1429, 1487
BstNSI RCATGY 1 cut(s) 1455
BstSFI CTRYAG 3 cut(s) 259, 530, 657
BstSNI TACGTA 1 cut(s) 1564
BstUI CGCG 2 cut(s) 372, 711
BstV1I GCAGC 3 cut(s) 382, 833, 1435
BstV2I GAAGAC 3 cut(s) 524, 1641, 1677
BstX2I RGATCY 1 cut(s) 1276
BstXI CCANNNNNNTGG 1 cut(s) 466
BstYI RGATCY 1 cut(s) 1276
Bsu15I ATCGAT 1 cut(s) 1057
BsuRI GGCC 5 cut(s) 754, 1423, 1471, 1531, 1619
BsuTUI ATCGAT 1 cut(s) 1057
BtgI CCRYGG 2 cut(s) 370, 1263
BtsCI GGATG 6 cut(s) 25, 205, 316, 848, 1238, 1640
BtsI GCAGTG 1 cut(s) 1443
BtsIMutI CAGTG 4 cut(s) 169, 466, 621, 1443
BveI ACCTGC 2 cut(s) 1500, 1595
Cac8I GCNNGC 6 cut(s) 304, 585, 1453, 1575, 1579, 1689
CciI TCATGA 1 cut(s) 1351
CfoI GCGC 2 cut(s) 713, 1663
Cfr10I RCCGGY 1 cut(s) 379
Cfr13I GGNCC 3 cut(s) 1470, 1530, 1596
Cfr42I CCGCGG 1 cut(s) 373
ClaI ATCGAT 1 cut(s) 1057
Csp6I GTAC 2 cut(s) 285, 599
CviQI GTAC 2 cut(s) 285, 599
DdeI CTNAG 6 cut(s) 27, 161, 196, 221, 624, 785
DpnI GATC 8 cut(s) 142, 295, 432, 609, 698, 858, 1278, 1702
DpnII GATC 8 cut(s) 140, 293, 430, 607, 696, 856, 1276, 1700
DraIII CACNNNGTG 2 cut(s) 164, 1157
DriI GACNNNNNGTC 1 cut(s) 525
EaeI YGGCCR 2 cut(s) 1421, 1617
Eam1104I CTCTTC 2 cut(s) 658, 1125
Eam1105I GACNNNNNGTC 1 cut(s) 525
EarI CTCTTC 2 cut(s) 658, 1125
Eco105I TACGTA 1 cut(s) 1564
Eco130I CCWWGG 2 cut(s) 1242, 1263
Eco32I GATATC 1 cut(s) 881
Eco47I GGWCC 1 cut(s) 1596
Eco47III AGCGCT 1 cut(s) 1662
Eco57I CTGAAG 2 cut(s) 351, 1089
Eco88I CYCGRG 1 cut(s) 1664
EcoRI GAATTC 1 cut(s) 1705
EcoRV GATATC 1 cut(s) 881
EcoT14I CCWWGG 2 cut(s) 1242, 1263
EcoT22I ATGCAT 1 cut(s) 1457
ErhI CCWWGG 2 cut(s) 1242, 1263
FalI AAGNNNNNCTT 2 cut(s) 910, 942
FaqI GGGAC 2 cut(s) 400, 440
FbaI TGATCA 1 cut(s) 430
Fnu4HI GCNGC 4 cut(s) 396, 776, 847, 1449
FokI GGATG 6 cut(s) 32, 192, 303, 855, 1225, 1627
Fsp4HI GCNGC 4 cut(s) 396, 776, 847, 1449
FspBI CTAG 4 cut(s) 291, 566, 588, 1392
GlaI GCGC 2 cut(s) 712, 1662
GluI GCNGC 4 cut(s) 396, 776, 847, 1449
GsuI CTGGAG 1 cut(s) 750
HaeII RGCGCY 1 cut(s) 1664
HaeIII GGCC 5 cut(s) 754, 1423, 1471, 1531, 1619
HapII CCGG 4 cut(s) 209, 380, 674, 1124
HhaI GCGC 2 cut(s) 713, 1663
Hin6I GCGC 2 cut(s) 711, 1661
HinP1I GCGC 2 cut(s) 711, 1661
HincII GTYRAC 1 cut(s) 1093
HindII GTYRAC 1 cut(s) 1093
HindIII AAGCTT 1 cut(s) 333
HinfI GANTC 8 cut(s) 53, 520, 558, 680, 1115, 1180, 1231, 1286
HpaII CCGG 4 cut(s) 209, 380, 674, 1124
HphI GGTGA 2 cut(s) 497, 659
Hpy166II GTNNAC 2 cut(s) 488, 1093
Hpy188I TCNGA 6 cut(s) 30, 123, 321, 412, 477, 802
Hpy188III TCNNGA 9 cut(s) 209, 514, 616, 684, 1124, 1290, 1352, 1459, 1588
Hpy8I GTNNAC 2 cut(s) 488, 1093
HpyAV CCTTC 7 cut(s) 261, 566, 760, 797, 836, 952, 998
HpyCH4III ACNGT 3 cut(s) 67, 1157, 1349
HpyCH4IV ACGT 1 cut(s) 1563
HpyF10VI GCNNNNNNNGC 3 cut(s) 1209, 1429, 1487
HpyF3I CTNAG 6 cut(s) 27, 161, 196, 221, 624, 785
HpySE526I ACGT 1 cut(s) 1563
HspAI GCGC 2 cut(s) 711, 1661
Kpn2I TCCGGA 2 cut(s) 208, 1123
Ksp22I TGATCA 1 cut(s) 430
KspI CCGCGG 1 cut(s) 373
Kzo9I GATC 8 cut(s) 140, 293, 430, 607, 696, 856, 1276, 1700
LmnI GCTCC 2 cut(s) 676, 1398
Lsp1109I GCAGC 3 cut(s) 382, 833, 1435
LweI GCATC 2 cut(s) 833, 1464
MaeI CTAG 4 cut(s) 291, 566, 588, 1392
MaeII ACGT 1 cut(s) 1563
MaeIII GTNAC 7 cut(s) 156, 1016, 1160, 1287, 1376, 1559, 1607
MalI GATC 8 cut(s) 142, 295, 432, 609, 698, 858, 1278, 1702
MboI GATC 8 cut(s) 140, 293, 430, 607, 696, 856, 1276, 1700
MboII GAAGA 7 cut(s) 529, 675, 898, 1082, 1112, 1646, 1682
MfeI CAATTG 1 cut(s) 390
MflI RGATCY 1 cut(s) 1276
MlsI TGGCCA 2 cut(s) 1423, 1619
MluNI TGGCCA 2 cut(s) 1423, 1619
MlyI GAGTC 3 cut(s) 514, 1189, 1295
MmeI TCCRAC 2 cut(s) 845, 1611
Mox20I TGGCCA 2 cut(s) 1423, 1619
Mph1103I ATGCAT 1 cut(s) 1457
MroI TCCGGA 2 cut(s) 208, 1123
MroXI GAANNNNTTC 1 cut(s) 962
MscI TGGCCA 2 cut(s) 1423, 1619
MseI TTAA 7 cut(s) 501, 632, 917, 939, 1035, 1043, 1322
Msp20I TGGCCA 2 cut(s) 1423, 1619
MspA1I CMGCKG 2 cut(s) 372, 1203
MspI CCGG 4 cut(s) 209, 380, 674, 1124
MunI CAATTG 1 cut(s) 390
Mva1269I GAATGC 1 cut(s) 764
MvnI CGCG 2 cut(s) 372, 711
MwoI GCNNNNNNNGC 3 cut(s) 1209, 1429, 1487
NcoI CCATGG 1 cut(s) 1263
NdeII GATC 8 cut(s) 140, 293, 430, 607, 696, 856, 1276, 1700
NlaIV GGNNCC 1 cut(s) 1472
NmeAIII GCCGAG 1 cut(s) 1528
NmuCI GTSAC 3 cut(s) 156, 1287, 1376
NsiI ATGCAT 1 cut(s) 1457
NspI RCATGY 1 cut(s) 1455
PaeI GCATGC 1 cut(s) 1455
PaeR7I CTCGAG 1 cut(s) 1664
PagI TCATGA 1 cut(s) 1351
PctI GAATGC 1 cut(s) 764
PdmI GAANNNNTTC 1 cut(s) 962
PfeI GAWTC 5 cut(s) 53, 558, 680, 1115, 1231
PflFI GACNNNGTC 3 cut(s) 452, 1130, 1594
PkrI GCNGC 4 cut(s) 397, 777, 848, 1450
PleI GAGTC 3 cut(s) 514, 1188, 1294
PpsI GAGTC 3 cut(s) 514, 1188, 1294
Ppu21I YACGTR 1 cut(s) 1564
PspN4I GGNNCC 1 cut(s) 1472
PspPI GGNCC 3 cut(s) 1470, 1530, 1596
PspXI VCTCGAGB 1 cut(s) 1664
PstI CTGCAG 1 cut(s) 534
PsuI RGATCY 1 cut(s) 1276
PsyI GACNNNGTC 3 cut(s) 452, 1130, 1594
RsaI GTAC 2 cut(s) 286, 600
RsaNI GTAC 2 cut(s) 285, 599
SacII CCGCGG 1 cut(s) 373
SaqAI TTAA 7 cut(s) 501, 632, 917, 939, 1035, 1043, 1322
SatI GCNGC 4 cut(s) 396, 776, 847, 1449
Sau3AI GATC 8 cut(s) 140, 293, 430, 607, 696, 856, 1276, 1700
Sau96I GGNCC 3 cut(s) 1470, 1530, 1596
ScaI AGTACT 1 cut(s) 600
SchI GAGTC 3 cut(s) 514, 1189, 1295
SfaNI GCATC 2 cut(s) 833, 1464
SfcI CTRYAG 3 cut(s) 259, 530, 657
Sfr274I CTCGAG 1 cut(s) 1664
Sfr303I CCGCGG 1 cut(s) 373
SgrBI CCGCGG 1 cut(s) 373
SinI GGWCC 1 cut(s) 1596
SlaI CTCGAG 1 cut(s) 1664
SmlI CTYRAG 2 cut(s) 997, 1664
SmoI CTYRAG 2 cut(s) 997, 1664
SnaBI TACGTA 1 cut(s) 1564
SphI GCATGC 1 cut(s) 1455
SsiI CCGC 4 cut(s) 370, 372, 775, 1201
SspI AATATT 2 cut(s) 127, 245
SspMI CTAG 4 cut(s) 291, 566, 588, 1392
StyI CCWWGG 2 cut(s) 1242, 1263
TaaI ACNGT 3 cut(s) 67, 1157, 1349
TaiI ACGT 1 cut(s) 1566
TaqI TCGA 5 cut(s) 202, 810, 1057, 1665, 1703
TatI WGTACW 2 cut(s) 284, 598
TauI GCSGC 1 cut(s) 778
TfiI GAWTC 5 cut(s) 53, 558, 680, 1115, 1231
Tru1I TTAA 7 cut(s) 501, 632, 917, 939, 1035, 1043, 1322
Tru9I TTAA 7 cut(s) 501, 632, 917, 939, 1035, 1043, 1322
TscAI CASTG 4 cut(s) 169, 466, 628, 1450
TseFI GTSAC 3 cut(s) 156, 1287, 1376
TseI GCWGC 3 cut(s) 395, 846, 1448
Tsp45I GTSAC 3 cut(s) 156, 1287, 1376
TspGWI ACGGA 2 cut(s) 515, 855
TspRI CASTG 4 cut(s) 169, 466, 628, 1450
Tth111I GACNNNGTC 3 cut(s) 452, 1130, 1594
VpaK11BI GGWCC 1 cut(s) 1596
XapI RAATTY 4 cut(s) 343, 891, 1583, 1705
XceI RCATGY 1 cut(s) 1455
XcmI CCANNNNNNNNNTGG 3 cut(s) 1270, 1431, 1627
XhoI CTCGAG 1 cut(s) 1664
XmnI GAANNNNTTC 1 cut(s) 962
XspI CTAG 4 cut(s) 291, 566, 588, 1392
ZrmI AGTACT 1 cut(s) 600
Zsp2I ATGCAT 1 cut(s) 1457
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.