FvH4_2g06540

Encoded by

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb2
OG0017980
Physical Location & Seq
Reverse (-)
5432354 .. 5433271
918 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_2g06540.t1

Sequence Viewer

Length: 918 bp
ATGGAACCCCCACCAATCACTGATGATGTAGAACATCAATTCTTCCCCTTCTTCAAAGTTTACAAAGATGGTCGTGTAGAGAGGTTCATGCCTTCTGAGAAACAACCTCCCTGTGATGACCCGAGCTCCACTGTCCGGTCCAACGATGTAATAATCTCCACCGAACCGGTTGTCTCAGCCCGGGTTTTCATGCCCCGATTACCCAACAACCCCGAAAAGAAGCTCCCTGTCGTGGTCTACATTCACGGTGGTGGCTTCTCAATAGCCTCCGCTTTCCACCCGGGTTATCACAAGTACGTGTCCTCCCTTGTCGCCCAAGCCAACGTCATCGCCGTCTCCGTCGACTATCGGCTAGCACCGGAGCACCCGATACCGGCATGCTACGAAGACTCGTGGGCGGTGCTCAATTGGGTGGCTTCCCATGCAAATGGGGATGGCCCTGATCCATGGCTGAACAGCCATGCAGACTTCGGTCGGGTCTTTTTGGCGGGTGACAGTGCCGGGGCGAATATTTCCCACAACATAGTATCTCGGGTCGGGTCAACTGGGTTACCCGGAGTGAAGATAGTAGGAGTGGCTTTGGTGCATCCTTATTTTGGTGGAACGGAAGATGATAAAATGTGGCTGTACATGTGTCCGGAGAACGGTGGATTGGATGACCCGAGGCTGAAACCGGCGGTGGAGGATCTGAGAAGACTAGGGTGTGAAAGGGTTTTGGTTTTTGTTGCCGGGAAAGACCATTTGAGGGAGAGAGGGACTAGTTATGTTGAGGCTTTGAAGGGAAGCGGGTGGAAAGGGACGGTGGAGATTGTAGAACATGAGGAGGAGAATCATGTCTTCCATCTGATGAAGCCGGAGTCTGAGAATGCTGTGGATTTGATGAGTAGGTTGGTCTCCTTCATCAATCAAGCAGGGTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Abhydrolase_3

Protein Analysis

306

Amino Acids

33.64

Weight (kDa)

5.51

Isoelectric Point (pI)

43.23

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
COesterase PF00135 62 - 120 2.6e-09 Carboxylesterase family
BD-FAE PF20434 67 - 172 3.5e-13 BD-FAE
Abhydrolase_3 PF07859 78 - 281 1.6e-46 alpha/beta hydrolase fold
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0017980)

Species Orthologous Gene IDs
fragaria_vesca FvH4_2g06540
rosa_chinensis RchiOBHm_Chr6g0258461
rosa_laevigata RLG00000014610 RLG00000014611
rosa_multiflora Rmu_sc0008229.1_g000008 Rmu_sc0008229.1_g000010
rosa_roxburghii Rroxscaffold_178G00437460 Rroxscaffold_7G00205710
rosa_rugosa Rorug05G0581800
rosa_samantha Rh6CG086700
rosa_wichuraiana Rw6G008510

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccI GTMKAC 2 cut(s) 237, 342
AccIII TCCGGA 1 cut(s) 637
AciI CCGC 5 cut(s) 270, 398, 488, 677, 786
AclWI GGATC 2 cut(s) 437, 693
AfaI GTAC 2 cut(s) 296, 629
AfiI CCNNNNNNNGG 6 cut(s) 135, 232, 373, 596, 644, 745
AflIII ACRYGT 2 cut(s) 297, 630
AgeI ACCGGT 1 cut(s) 166
AgsI TTSAA 2 cut(s) 55, 778
AhlI ACTAGT 1 cut(s) 758
AjuI GAANNNNNNNTTGG 2 cut(s) 635, 667
AleI CACNNNNGTG 1 cut(s) 249
AluBI AGCT 2 cut(s) 126, 223
AluI AGCT 2 cut(s) 126, 223
Alw21I GWGCWC 3 cut(s) 128, 366, 405
Alw26I GTCTC 3 cut(s) 178, 340, 898
AlwI GGATC 2 cut(s) 437, 693
Ama87I CYCGRG 5 cut(s) 121, 180, 280, 531, 661
Aor13HI TCCGGA 1 cut(s) 637
AoxI GGCC 1 cut(s) 436
ArsI GACNNNNNNTTYG 2 cut(s) 156, 188
AsiGI ACCGGT 1 cut(s) 166
AspS9I GGNCC 2 cut(s) 138, 437
AsuC2I CCSGG 7 cut(s) 181, 182, 281, 282, 502, 555, 730
AsuHPI GGTGA 1 cut(s) 503
AsuNHI GCTAGC 1 cut(s) 352
AvaI CYCGRG 5 cut(s) 121, 180, 280, 531, 661
AvaII GGWCC 1 cut(s) 138
BanII GRGCYC 1 cut(s) 128
BauI CACGAG 1 cut(s) 391
BbsI GAAGAC 3 cut(s) 393, 700, 829
Bbv12I GWGCWC 3 cut(s) 128, 366, 405
BccI CCATC 3 cut(s) 62, 428, 849
BceAI ACGGC 1 cut(s) 317
BcnI CCSGG 7 cut(s) 181, 182, 281, 282, 502, 555, 730
BcoDI GTCTC 3 cut(s) 178, 340, 898
BcuI ACTAGT 1 cut(s) 758
BfaI CTAG 3 cut(s) 353, 698, 759
Bme1390I CCNGG 7 cut(s) 181, 182, 281, 282, 502, 555, 730
Bme18I GGWCC 1 cut(s) 138
BmeT110I CYCGRG 5 cut(s) 121, 180, 280, 531, 661
BmgT120I GGNCC 2 cut(s) 138, 437
BmiI GGNNCC 1 cut(s) 6
BmrFI CCNGG 7 cut(s) 181, 182, 281, 282, 502, 555, 730
BmrI ACTGGG 1 cut(s) 555
BmsI GCATC 1 cut(s) 595
BmtI GCTAGC 1 cut(s) 356
BmuI ACTGGG 1 cut(s) 555
BoxI GACNNNNGTC 1 cut(s) 471
BpiI GAAGAC 3 cut(s) 393, 700, 829
BpuMI CCSGG 7 cut(s) 181, 182, 281, 282, 502, 555, 730
BsaAI YACGTR 1 cut(s) 298
BsaI GGTCTC 1 cut(s) 898
BsaJI CCNNGG 5 cut(s) 180, 280, 446, 501, 662
BsaWI WCCGGW 4 cut(s) 135, 166, 358, 637
BsaXI ACNNNNNCTCC 4 cut(s) 110, 140, 287, 317
Bsc4I CCNNNNNNNGG 6 cut(s) 135, 232, 373, 596, 644, 745
Bse118I RCCGGY 3 cut(s) 166, 373, 673
Bse1I ACTGG 1 cut(s) 550
BseAI TCCGGA 1 cut(s) 637
BseDI CCNNGG 5 cut(s) 180, 280, 446, 501, 662
BseGI GGATG 3 cut(s) 439, 586, 661
BseLI CCNNNNNNNGG 6 cut(s) 135, 232, 373, 596, 644, 745
BseMII CTCAG 4 cut(s) 87, 189, 680, 852
BseNI ACTGG 1 cut(s) 550
BseRI GAGGAG 2 cut(s) 836, 839
Bsh1285I CGRYCG 1 cut(s) 475
BshFI GGCC 1 cut(s) 438
BshTI ACCGGT 1 cut(s) 166
BsiEI CGRYCG 1 cut(s) 475
BsiHKAI GWGCWC 3 cut(s) 128, 366, 405
BsiHKCI CYCGRG 5 cut(s) 121, 180, 280, 531, 661
BslFI GGGAC 2 cut(s) 769, 811
BslI CCNNNNNNNGG 6 cut(s) 135, 232, 373, 596, 644, 745
BsmAI GTCTC 3 cut(s) 178, 340, 898
BsmBI CGTCTC 1 cut(s) 340
BsmFI GGGAC 2 cut(s) 769, 811
BsmI GAATGC 1 cut(s) 871
BsnI GGCC 1 cut(s) 438
Bso31I GGTCTC 1 cut(s) 898
BsoBI CYCGRG 5 cut(s) 121, 180, 280, 531, 661
Bsp1286I GDGCHC 3 cut(s) 128, 366, 405
Bsp13I TCCGGA 1 cut(s) 637
Bsp1407I TGTACA 1 cut(s) 627
Bsp143I GATC 2 cut(s) 442, 685
Bsp19I CCATGG 1 cut(s) 446
BspACI CCGC 5 cut(s) 270, 398, 488, 677, 786
BspANI GGCC 1 cut(s) 438
BspCNI CTCAG 4 cut(s) 88, 188, 681, 853
BspEI TCCGGA 1 cut(s) 637
BspLI GGNNCC 1 cut(s) 6
BspOI GCTAGC 1 cut(s) 356
BspPI GGATC 2 cut(s) 437, 693
BspTNI GGTCTC 1 cut(s) 898
BsrFI RCCGGY 3 cut(s) 166, 373, 673
BsrGI TGTACA 1 cut(s) 627
BsrI ACTGG 1 cut(s) 550
BssAI RCCGGY 3 cut(s) 166, 373, 673
BssECI CCNNGG 5 cut(s) 180, 280, 446, 501, 662
BssMI GATC 2 cut(s) 442, 685
BssSI CACGAG 1 cut(s) 391
BssT1I CCWWGG 1 cut(s) 446
Bst2BI CACGAG 1 cut(s) 391
Bst4CI ACNGT 5 cut(s) 133, 248, 497, 647, 802
BstAUI TGTACA 1 cut(s) 627
BstBAI YACGTR 1 cut(s) 298
BstC8I GCNNGC 2 cut(s) 354, 379
BstDEI CTNAG 4 cut(s) 96, 175, 689, 861
BstDSI CCRYGG 1 cut(s) 446
BstEII GGTNACC 1 cut(s) 549
BstF5I GGATG 3 cut(s) 439, 586, 661
BstKTI GATC 2 cut(s) 445, 688
BstMAI GTCTC 3 cut(s) 178, 340, 898
BstMBI GATC 2 cut(s) 442, 685
BstMCI CGRYCG 1 cut(s) 475
BstMWI GCNNNNNNNGC 1 cut(s) 422
BstNSI RCATGY 2 cut(s) 381, 634
BstPAI GACNNNNGTC 1 cut(s) 471
BstPI GGTNACC 1 cut(s) 549
BstSCI CCNGG 7 cut(s) 179, 180, 279, 280, 500, 553, 728
BstV2I GAAGAC 3 cut(s) 393, 700, 829
BstX2I RGATCY 1 cut(s) 685
BstXI CCANNNNNNTGG 1 cut(s) 428
BstYI RGATCY 1 cut(s) 685
BsuRI GGCC 1 cut(s) 438
BtgI CCRYGG 1 cut(s) 446
BtgZI GCGATG 1 cut(s) 313
BtsCI GGATG 3 cut(s) 439, 586, 661
BtsIMutI CAGTG 3 cut(s) 18, 129, 502
Cac8I GCNNGC 2 cut(s) 354, 379
Cfr10I RCCGGY 3 cut(s) 166, 373, 673
Cfr13I GGNCC 2 cut(s) 138, 437
Cfr9I CCCGGG 2 cut(s) 180, 280
Csp6I GTAC 2 cut(s) 295, 628
CspAI ACCGGT 1 cut(s) 166
CviAII CATG 9 cut(s) 88, 190, 378, 422, 447, 461, 631, 818, 833
CviQI GTAC 2 cut(s) 295, 628
DdeI CTNAG 4 cut(s) 96, 175, 689, 861
DpnI GATC 2 cut(s) 444, 687
DpnII GATC 2 cut(s) 442, 685
Ecl136II GAGCTC 1 cut(s) 126
Eco130I CCWWGG 1 cut(s) 446
Eco24I GRGCYC 1 cut(s) 128
Eco31I GGTCTC 1 cut(s) 898
Eco47I GGWCC 1 cut(s) 138
Eco53kI GAGCTC 1 cut(s) 126
Eco88I CYCGRG 5 cut(s) 121, 180, 280, 531, 661
Eco91I GGTNACC 1 cut(s) 549
EcoICRI GAGCTC 1 cut(s) 126
EcoO65I GGTNACC 1 cut(s) 549
EcoT14I CCWWGG 1 cut(s) 446
EcoT38I GRGCYC 1 cut(s) 128
ErhI CCWWGG 1 cut(s) 446
Esp3I CGTCTC 1 cut(s) 340
FaeI CATG 9 cut(s) 91, 193, 381, 425, 450, 464, 634, 821, 836
FaqI GGGAC 2 cut(s) 769, 811
FatI CATG 9 cut(s) 87, 189, 377, 421, 446, 460, 630, 817, 832
FauI CCCGC 2 cut(s) 481, 779
FblI GTMKAC 2 cut(s) 237, 342
FokI GGATG 3 cut(s) 446, 573, 668
FriOI GRGCYC 1 cut(s) 128
FspBI CTAG 3 cut(s) 353, 698, 759
HaeIII GGCC 1 cut(s) 438
Hin1II CATG 9 cut(s) 91, 193, 381, 425, 450, 464, 634, 821, 836
HincII GTYRAC 2 cut(s) 343, 543
HindII GTYRAC 2 cut(s) 343, 543
HinfI GANTC 3 cut(s) 389, 829, 857
HphI GGTGA 1 cut(s) 503
Hpy166II GTNNAC 4 cut(s) 61, 238, 343, 543
Hpy188I TCNGA 4 cut(s) 97, 690, 846, 862
Hpy188III TCNNGA 1 cut(s) 638
Hpy8I GTNNAC 4 cut(s) 61, 238, 343, 543
Hpy99I CGWCG 1 cut(s) 344
HpyAV CCTTC 4 cut(s) 58, 102, 772, 907
HpyCH4III ACNGT 5 cut(s) 133, 248, 497, 647, 802
HpyCH4IV ACGT 2 cut(s) 297, 324
HpyCH4V TGCA 3 cut(s) 425, 464, 586
HpyF10VI GCNNNNNNNGC 1 cut(s) 422
HpyF3I CTNAG 4 cut(s) 96, 175, 689, 861
HpySE526I ACGT 2 cut(s) 297, 324
Hsp92II CATG 9 cut(s) 91, 193, 381, 425, 450, 464, 634, 821, 836
Kpn2I TCCGGA 1 cut(s) 637
Kzo9I GATC 2 cut(s) 442, 685
LmnI GCTCC 3 cut(s) 131, 228, 361
LweI GCATC 1 cut(s) 595
MaeI CTAG 3 cut(s) 353, 698, 759
MaeII ACGT 2 cut(s) 297, 324
MaeIII GTNAC 2 cut(s) 491, 549
MalI GATC 2 cut(s) 444, 687
MboI GATC 2 cut(s) 442, 685
MboII GAAGA 7 cut(s) 34, 43, 398, 574, 620, 705, 829
MfeI CAATTG 1 cut(s) 406
MflI RGATCY 1 cut(s) 685
MhlI GDGCHC 3 cut(s) 128, 366, 405
MluCI AATT 2 cut(s) 38, 406
MlyI GAGTC 2 cut(s) 383, 866
MmeI TCCRAC 1 cut(s) 165
MroI TCCGGA 1 cut(s) 637
MslI CAYNNNNRTG 2 cut(s) 249, 426
MspR9I CCNGG 7 cut(s) 181, 182, 281, 282, 502, 555, 730
MunI CAATTG 1 cut(s) 406
Mva1269I GAATGC 1 cut(s) 871
MwoI GCNNNNNNNGC 1 cut(s) 422
NciI CCSGG 7 cut(s) 181, 182, 281, 282, 502, 555, 730
NcoI CCATGG 1 cut(s) 446
NdeII GATC 2 cut(s) 442, 685
NheI GCTAGC 1 cut(s) 352
NlaIII CATG 9 cut(s) 91, 193, 381, 425, 450, 464, 634, 821, 836
NlaIV GGNNCC 1 cut(s) 6
NmuCI GTSAC 1 cut(s) 491
NspI RCATGY 2 cut(s) 381, 634
OliI CACNNNNGTG 1 cut(s) 249
PaeI GCATGC 1 cut(s) 381
PciI ACATGT 1 cut(s) 630
PcsI WCGNNNNNNNCGW 2 cut(s) 330, 336
PctI GAATGC 1 cut(s) 871
PfeI GAWTC 1 cut(s) 829
PinAI ACCGGT 1 cut(s) 166
PleI GAGTC 2 cut(s) 383, 865
PpsI GAGTC 2 cut(s) 383, 865
Ppu21I YACGTR 1 cut(s) 298
PscI ACATGT 1 cut(s) 630
PshAI GACNNNNGTC 1 cut(s) 471
Psp124BI GAGCTC 1 cut(s) 128
PspEI GGTNACC 1 cut(s) 549
PspN4I GGNNCC 1 cut(s) 6
PspPI GGNCC 2 cut(s) 138, 437
PsuI RGATCY 1 cut(s) 685
RsaI GTAC 2 cut(s) 296, 629
RsaNI GTAC 2 cut(s) 295, 628
RseI CAYNNNNRTG 2 cut(s) 249, 426
SacI GAGCTC 1 cut(s) 128
SalI GTCGAC 1 cut(s) 341
Sau3AI GATC 2 cut(s) 442, 685
Sau96I GGNCC 2 cut(s) 138, 437
SchI GAGTC 2 cut(s) 383, 866
ScrFI CCNGG 7 cut(s) 181, 182, 281, 282, 502, 555, 730
SduI GDGCHC 3 cut(s) 128, 366, 405
SetI ASST 7 cut(s) 86, 109, 128, 225, 300, 327, 890
SfaNI GCATC 1 cut(s) 595
SinI GGWCC 1 cut(s) 138
SmaI CCCGGG 2 cut(s) 182, 282
SmiMI CAYNNNNRTG 2 cut(s) 249, 426
SpeI ACTAGT 1 cut(s) 758
SphI GCATGC 1 cut(s) 381
Sse9I AATT 2 cut(s) 38, 406
SsiI CCGC 5 cut(s) 270, 398, 488, 677, 786
SspI AATATT 1 cut(s) 511
SspMI CTAG 3 cut(s) 353, 698, 759
SstI GAGCTC 1 cut(s) 128
StyD4I CCNGG 7 cut(s) 179, 180, 279, 280, 500, 553, 728
StyI CCWWGG 1 cut(s) 446
TaaI ACNGT 5 cut(s) 133, 248, 497, 647, 802
TaiI ACGT 2 cut(s) 300, 327
TaqI TCGA 1 cut(s) 342
TaqII GACCGA 1 cut(s) 461
TasI AATT 2 cut(s) 38, 406
TatI WGTACW 1 cut(s) 627
TfiI GAWTC 1 cut(s) 829
TscAI CASTG 3 cut(s) 25, 136, 502
TseFI GTSAC 1 cut(s) 491
Tsp45I GTSAC 1 cut(s) 491
TspDTI ATGAA 4 cut(s) 76, 178, 863, 889
TspGWI ACGGA 2 cut(s) 328, 620
TspMI CCCGGG 2 cut(s) 180, 280
TspRI CASTG 3 cut(s) 25, 136, 502
VpaK11BI GGWCC 1 cut(s) 138
XceI RCATGY 2 cut(s) 381, 634
XmaI CCCGGG 2 cut(s) 180, 280
XmiI GTMKAC 2 cut(s) 237, 342
XspI CTAG 3 cut(s) 353, 698, 759
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.