FvH4_2g10120

MatE

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb2
OG0016752
Physical Location & Seq
Reverse (-)
8957106 .. 8964215
7110 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_2g10120.t3

Sequence Viewer

Length: 1629 bp
ATGAATCTGAAAACCATGGCGCTCCATTCACACAGTGCTCACCTCCCAACCCATAAGCGCTTCTCTCGCCCTTCAGCTTTACACAACCCATTTCAGACCTCTCATGTCTTCCGCAGCTCTGTTCGTTACGACAGTAACCCGACAAGTTTCCCTGTTTTGGTGGCGAGAAATAAGGGCGGGAGGAGATTAGTTACGGCGTGCATGGGTAATGCTGACTCAGAAGAGGAGGAGGAGCCAGTGTTGGTGGCTGAGAAGAAGGAGGAGTTGGTGGAGCGAAGCCTATGGAAGCAGGTGAAGGACATTGTCATGTTTTCGGGTCCGGCTACCGGGTTGTGGATTTGTGGGCCGTTGATGAGTCTCATCTCCACCGCTGTTATCGGCCAGGCTAGCTCTACTGAGCTTGCTGCTTTGGGTCCGGGAACTGTCTTCTGTGACAACATGAATCTTTTGTTCATGTTCCTCTCCATTGCTACTTCTAACATGGTTGCCACTGCGCTCGCCAAGAGGGATAAAAAGGAATTGCAGCATCAGATATCTATCTTGCTCTTCGTTGGACTGACTTGTGGAGTTCTGATGCTCTTGTTTACTCAATTCCATGGTTCATGGGCGCTAACAGCTTTTGCTGGGCCCAAGAATGCACATATTATACCTGCAGCAAGCAAATATGTTCAGATTCGAGGGTTAGCGTGGCCTGCGATTCTTTTCGGATTGGTTGCTCAAAGCGCAAGTCTGGGCATGAAAGATTCATTGGGACCTTTAAAGGCTCTGGTGGTTGCCAGTGTTGTGAATGGCCTTGGTCATGTAGTCCTATGCAATTTCTTACACTATGGTATTACTGGTGCCGCATGGGCAACAATGGCATCTCAGATTGTTGCCGCTGGTATGATGATTGAAGCTCTGAATAAGAAAGGATTCAGTGCATTTTCCATCTCTGTTCCATCACCTCGTGAATGCCTGCAAATATTCGGAATTGCAGGTCCGGTGTTTGTAACAATGTTCTCAAAGGTGGCTTTCTACTCACTGATGACATACTTTGCTACTGCTATGGGTACAAATATAGTTGCTGCTCATCAGGTCATGATACAAATGTACGGGATGTGTGTGGTATGGGGTGAGCCTCTTTCTCAAACTGCACAATCATTTATGCCTGAGTTATTATATGGAGTTGACCGAAGTTTGGAGAAGGCCCGCATGCTGCTAAAGTCACTCGTAATCATTGGAGCTATTCTTGGACTGGCAATAGGAGCTATTGGGATGTCTGTTCCTTGGTTATTCCCCAATATCTTTACATTTGATCTAAAAGTCATACAAGAGATGCACAAAGTATTGTTTCTGTTCTTCCTGTCGTTATCTATTACACCCTGTACTCACAGCCTTGAGGGCACATTGCTGGCTGGACGTGATCTTACCTTCATCAGCTTCTCAATGAGCAGTTGCTTTTCCTTGGGTGCTCTTCTATTGCTGCTTTTGAACAGCAGAGGATTTGGTTTGCCAGGCTGCTGGTTTGCACTTGTTGGCTTTCAATGGGCTCGATTTTTCCTTGCTCTCCGGCGGCTTCTTTCTCCCAATGGCATGCTCCACACAGAAGAGCTTGGCTCCTATAACGTGGGAAAGTTGAAAGCTACTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
MatE

Protein Analysis

543

Amino Acids

58.91

Weight (kDa)

9.24

Isoelectric Point (pI)

45.53

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
MatE PF01554 114 - 263 3.7e-15 MatE
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0016752)

Species Orthologous Gene IDs
fragaria_vesca FvH4_2g10120 FvH4_2g10120 FvH4_2g10120 FvH4_2g10120
rosa_chinensis RchiOBHm_Chr6g0267191
rosa_laevigata RLG00000014020
rosa_multiflora Rmu_sc0000908.1_g000024 Rmu_ssc0000388.1_g000047
rosa_roxburghii Rroxscaffold_7G00201000
rosa_rugosa Rorug06G0030400
rosa_samantha Rh6BG152600 Rh6CG148500 Rh6DG136800
rosa_wichuraiana Rw6G013140

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 280
Acc36I ACCTGC 3 cut(s) 280, 658, 965
AccB1I GGYRCC 1 cut(s) 839
AciI CCGC 7 cut(s) 112, 177, 369, 843, 876, 1189, 1552
AcoI YGGCCR 1 cut(s) 379
AcuI CTGAAG 1 cut(s) 57
AdeI CACNNNGTG 2 cut(s) 35, 947
AfaI GTAC 3 cut(s) 1051, 1091, 1366
AfeI AGCGCT 1 cut(s) 59
AfiI CCNNNNNNNGG 4 cut(s) 157, 326, 333, 1177
AgsI TTSAA 4 cut(s) 893, 1471, 1523, 1618
AjiI CACGTC 1 cut(s) 1400
AjnI CCWGG 2 cut(s) 381, 1492
AjuI GAANNNNNNNTTGG 4 cut(s) 248, 280, 731, 763
Alw21I GWGCWC 2 cut(s) 40, 1453
Alw26I GTCTC 1 cut(s) 362
Aor51HI AGCGCT 1 cut(s) 59
AoxI GGCC 6 cut(s) 344, 379, 626, 689, 790, 1185
ApaI GGGCCC 1 cut(s) 630
ApeKI GCWGC 8 cut(s) 114, 404, 523, 653, 1064, 1195, 1462, 1497
ArsI GACNNNNNNTTYG 2 cut(s) 712, 744
Asp700I GAANNNNTTC 1 cut(s) 911
AspLEI GCGC 5 cut(s) 22, 60, 496, 610, 725
AspS9I GGNCC 8 cut(s) 317, 344, 413, 626, 627, 752, 977, 1186
AsuC2I CCSGG 2 cut(s) 328, 417
AsuHPI GGTGA 4 cut(s) 32, 304, 933, 1124
AsuNHI GCTAGC 1 cut(s) 386
AvaII GGWCC 4 cut(s) 317, 413, 752, 977
BaeGI GKGCMC 2 cut(s) 630, 1385
BaeI ACNNNNGTAYC 2 cut(s) 1073, 1106
BanI GGYRCC 1 cut(s) 839
BanII GRGCYC 2 cut(s) 630, 1531
BauI CACGAG 1 cut(s) 945
BbsI GAAGAC 2 cut(s) 100, 418
Bbv12I GWGCWC 2 cut(s) 40, 1453
BbvI GCAGC 8 cut(s) 126, 391, 535, 665, 1051, 1182, 1449, 1484
BccI CCATC 2 cut(s) 935, 946
BceAI ACGGC 2 cut(s) 210, 331
BciT130I CCWGG 2 cut(s) 383, 1494
BcnI CCSGG 2 cut(s) 328, 417
BcoDI GTCTC 1 cut(s) 362
BfaI CTAG 1 cut(s) 387
BfmI CTRYAG 1 cut(s) 651
BfoI RGCGCY 3 cut(s) 23, 61, 611
BfuAI ACCTGC 3 cut(s) 280, 658, 965
BglI GCCNNNNNGGC 2 cut(s) 848, 1380
Bme1390I CCNGG 4 cut(s) 328, 383, 417, 1494
Bme18I GGWCC 4 cut(s) 317, 413, 752, 977
BmgBI CACGTC 1 cut(s) 1400
BmgT120I GGNCC 8 cut(s) 317, 344, 413, 626, 627, 752, 977, 1186
BmiI GGNNCC 7 cut(s) 234, 318, 414, 628, 753, 841, 1597
BmrFI CCNGG 4 cut(s) 328, 383, 417, 1494
BmsI GCATC 4 cut(s) 535, 564, 869, 1305
BmtI GCTAGC 1 cut(s) 390
BpiI GAAGAC 2 cut(s) 100, 418
BplI GAGNNNNNCTC 2 cut(s) 1580, 1612
BpuEI CTTGAG 1 cut(s) 1397
BpuMI CCSGG 2 cut(s) 328, 417
BsaBI GATNNNNATC 1 cut(s) 536
BsaJI CCNNGG 5 cut(s) 15, 595, 793, 1265, 1443
BsaWI WCCGGW 1 cut(s) 979
BsaXI ACNNNNNCTCC 2 cut(s) 224, 254
Bsc4I CCNNNNNNNGG 4 cut(s) 157, 326, 333, 1177
Bse1I ACTGG 4 cut(s) 236, 777, 841, 1239
Bse3DI GCAATG 2 cut(s) 465, 1385
Bse8I GATNNNNATC 1 cut(s) 536
BseBI CCWGG 2 cut(s) 383, 1494
BseDI CCNNGG 5 cut(s) 15, 595, 793, 1265, 1443
BseGI GGATG 2 cut(s) 1101, 1260
BseJI GATNNNNATC 1 cut(s) 536
BseLI CCNNNNNNNGG 4 cut(s) 157, 326, 333, 1177
BseMI GCAATG 2 cut(s) 465, 1385
BseMII CTCAG 5 cut(s) 231, 240, 387, 878, 1140
BseNI ACTGG 4 cut(s) 236, 777, 841, 1239
BseRI GAGGAG 5 cut(s) 196, 239, 242, 245, 275
BseSI GKGCMC 2 cut(s) 630, 1385
BseXI GCAGC 8 cut(s) 126, 391, 535, 665, 1051, 1182, 1449, 1484
BseYI CCCAGC 1 cut(s) 623
BsgI GTGCAG 1 cut(s) 1116
BshFI GGCC 6 cut(s) 346, 381, 628, 691, 792, 1187
BshNI GGYRCC 1 cut(s) 839
BsiHKAI GWGCWC 2 cut(s) 40, 1453
BsiSI CCGG 5 cut(s) 320, 327, 416, 980, 1549
BslFI GGGAC 1 cut(s) 765
BslI CCNNNNNNNGG 4 cut(s) 157, 326, 333, 1177
BsmAI GTCTC 1 cut(s) 362
BsmFI GGGAC 1 cut(s) 765
BsmI GAATGC 2 cut(s) 640, 956
BsnI GGCC 6 cut(s) 346, 381, 628, 691, 792, 1187
Bsp120I GGGCCC 1 cut(s) 626
Bsp1286I GDGCHC 5 cut(s) 40, 630, 1385, 1453, 1531
Bsp143I GATC 2 cut(s) 1294, 1402
Bsp19I CCATGG 2 cut(s) 15, 595
BspACI CCGC 7 cut(s) 112, 177, 369, 843, 876, 1189, 1552
BspANI GGCC 6 cut(s) 346, 381, 628, 691, 792, 1187
BspCNI CTCAG 5 cut(s) 230, 241, 388, 877, 1141
BspHI TCATGA 1 cut(s) 1077
BspLI GGNNCC 7 cut(s) 234, 318, 414, 628, 753, 841, 1597
BspMAI CTGCAG 1 cut(s) 655
BspMI ACCTGC 3 cut(s) 280, 658, 965
BspOI GCTAGC 1 cut(s) 390
BspQI GCTCTTC 3 cut(s) 551, 1458, 1581
BspT107I GGYRCC 1 cut(s) 839
BsrDI GCAATG 2 cut(s) 465, 1385
BsrI ACTGG 4 cut(s) 236, 777, 841, 1239
BssECI CCNNGG 5 cut(s) 15, 595, 793, 1265, 1443
BssMI GATC 2 cut(s) 1294, 1402
BssSI CACGAG 1 cut(s) 945
BssT1I CCWWGG 5 cut(s) 15, 595, 793, 1265, 1443
Bst2BI CACGAG 1 cut(s) 945
Bst2UI CCWGG 2 cut(s) 383, 1494
Bst4CI ACNGT 3 cut(s) 35, 134, 424
Bst6I CTCTTC 4 cut(s) 216, 551, 1458, 1581
BstDEI CTNAG 5 cut(s) 217, 249, 396, 864, 1149
BstDSI CCRYGG 2 cut(s) 15, 595
BstF5I GGATG 2 cut(s) 1101, 1260
BstH2I RGCGCY 3 cut(s) 23, 61, 611
BstHHI GCGC 5 cut(s) 22, 60, 496, 610, 725
BstKTI GATC 2 cut(s) 1297, 1405
BstMAI GTCTC 1 cut(s) 362
BstMBI GATC 2 cut(s) 1294, 1402
BstMWI GCNNNNNNNGC 9 cut(s) 66, 387, 614, 692, 722, 848, 857, 1244, 1380
BstNI CCWGG 2 cut(s) 383, 1494
BstNSI RCATGY 2 cut(s) 1195, 1576
BstSCI CCNGG 4 cut(s) 326, 381, 415, 1492
BstSFI CTRYAG 1 cut(s) 651
BstSLI GKGCMC 2 cut(s) 630, 1385
BstV1I GCAGC 8 cut(s) 126, 391, 535, 665, 1051, 1182, 1449, 1484
BstV2I GAAGAC 2 cut(s) 100, 418
BstXI CCANNNNNNTGG 1 cut(s) 1500
BsuRI GGCC 6 cut(s) 346, 381, 628, 691, 792, 1187
BtgI CCRYGG 2 cut(s) 15, 595
BtrI CACGTC 1 cut(s) 1400
BtsCI GGATG 2 cut(s) 1101, 1260
BtsI GCAGTG 1 cut(s) 489
BtsIMutI CAGTG 6 cut(s) 40, 243, 489, 784, 922, 1019
BveI ACCTGC 3 cut(s) 280, 658, 965
CciI TCATGA 1 cut(s) 1077
CfoI GCGC 5 cut(s) 22, 60, 496, 610, 725
Cfr13I GGNCC 8 cut(s) 317, 344, 413, 626, 627, 752, 977, 1186
Csp6I GTAC 3 cut(s) 1050, 1090, 1365
CviQI GTAC 3 cut(s) 1050, 1090, 1365
DdeI CTNAG 5 cut(s) 217, 249, 396, 864, 1149
DpnI GATC 2 cut(s) 1296, 1404
DpnII GATC 2 cut(s) 1294, 1402
DraI TTTAAA 1 cut(s) 759
DraIII CACNNNGTG 2 cut(s) 35, 947
EaeI YGGCCR 1 cut(s) 379
Eam1104I CTCTTC 4 cut(s) 216, 551, 1458, 1581
EarI CTCTTC 4 cut(s) 216, 551, 1458, 1581
Eco130I CCWWGG 5 cut(s) 15, 595, 793, 1265, 1443
Eco24I GRGCYC 2 cut(s) 630, 1531
Eco32I GATATC 1 cut(s) 534
Eco47I GGWCC 4 cut(s) 317, 413, 752, 977
Eco47III AGCGCT 1 cut(s) 59
Eco57I CTGAAG 1 cut(s) 57
EcoO109I RGGNCCY 1 cut(s) 752
EcoRII CCWGG 2 cut(s) 381, 1492
EcoRV GATATC 1 cut(s) 534
EcoT14I CCWWGG 5 cut(s) 15, 595, 793, 1265, 1443
EcoT38I GRGCYC 2 cut(s) 630, 1531
ErhI CCWWGG 5 cut(s) 15, 595, 793, 1265, 1443
FaqI GGGAC 1 cut(s) 765
FauI CCCGC 2 cut(s) 170, 1196
FokI GGATG 2 cut(s) 1108, 1267
FriOI GRGCYC 2 cut(s) 630, 1531
FspBI CTAG 1 cut(s) 387
GlaI GCGC 5 cut(s) 21, 59, 495, 609, 724
GsaI CCCAGC 1 cut(s) 627
HaeII RGCGCY 3 cut(s) 23, 61, 611
HaeIII GGCC 6 cut(s) 346, 381, 628, 691, 792, 1187
HapII CCGG 5 cut(s) 320, 327, 416, 980, 1549
HhaI GCGC 5 cut(s) 22, 60, 496, 610, 725
Hin6I GCGC 5 cut(s) 20, 58, 494, 608, 723
HinP1I GCGC 5 cut(s) 20, 58, 494, 608, 723
HincII GTYRAC 1 cut(s) 1168
HindII GTYRAC 1 cut(s) 1168
HinfI GANTC 8 cut(s) 4, 215, 355, 442, 673, 697, 743, 912
HpaII CCGG 5 cut(s) 320, 327, 416, 980, 1549
HphI GGTGA 4 cut(s) 32, 304, 933, 1124
Hpy166II GTNNAC 2 cut(s) 585, 1168
Hpy188III TCNNGA 2 cut(s) 947, 1078
Hpy8I GTNNAC 2 cut(s) 585, 1168
HpyAV CCTTC 5 cut(s) 81, 250, 289, 1177, 1420
HpyCH4III ACNGT 3 cut(s) 35, 134, 424
HpyCH4IV ACGT 2 cut(s) 1399, 1605
HpyF10VI GCNNNNNNNGC 9 cut(s) 66, 387, 614, 692, 722, 848, 857, 1244, 1380
HpyF3I CTNAG 5 cut(s) 217, 249, 396, 864, 1149
HpySE526I ACGT 2 cut(s) 1399, 1605
HspAI GCGC 5 cut(s) 20, 58, 494, 608, 723
Kzo9I GATC 2 cut(s) 1294, 1402
LguI GCTCTTC 3 cut(s) 551, 1458, 1581
LmnI GCTCC 7 cut(s) 27, 232, 271, 1220, 1244, 1581, 1601
Lsp1109I GCAGC 8 cut(s) 126, 391, 535, 665, 1051, 1182, 1449, 1484
LweI GCATC 4 cut(s) 535, 564, 869, 1305
MaeI CTAG 1 cut(s) 387
MaeII ACGT 2 cut(s) 1399, 1605
MaeIII GTNAC 6 cut(s) 125, 134, 190, 431, 988, 1203
MalI GATC 2 cut(s) 1296, 1404
MboI GATC 2 cut(s) 1294, 1402
MboII GAAGA 8 cut(s) 100, 233, 265, 418, 538, 1330, 1445, 1598
MhlI GDGCHC 5 cut(s) 40, 630, 1385, 1453, 1531
MluCI AATT 4 cut(s) 518, 590, 814, 969
MlyI GAGTC 2 cut(s) 209, 364
MmeI TCCRAC 1 cut(s) 532
MroXI GAANNNNTTC 1 cut(s) 911
MseI TTAA 1 cut(s) 758
MslI CAYNNNNRTG 1 cut(s) 305
MspA1I CMGCKG 2 cut(s) 371, 878
MspI CCGG 5 cut(s) 320, 327, 416, 980, 1549
MspR9I CCNGG 4 cut(s) 328, 383, 417, 1494
Mva1269I GAATGC 2 cut(s) 640, 956
MvaI CCWGG 2 cut(s) 383, 1494
MwoI GCNNNNNNNGC 9 cut(s) 66, 387, 614, 692, 722, 848, 857, 1244, 1380
NciI CCSGG 2 cut(s) 328, 417
NcoI CCATGG 2 cut(s) 15, 595
NdeII GATC 2 cut(s) 1294, 1402
NheI GCTAGC 1 cut(s) 386
NlaIV GGNNCC 7 cut(s) 234, 318, 414, 628, 753, 841, 1597
NmuCI GTSAC 2 cut(s) 431, 1203
NspI RCATGY 2 cut(s) 1195, 1576
PaeI GCATGC 2 cut(s) 1195, 1576
PagI TCATGA 1 cut(s) 1077
PaqCI CACCTGC 1 cut(s) 280
PciSI GCTCTTC 3 cut(s) 551, 1458, 1581
PctI GAATGC 2 cut(s) 640, 956
PdmI GAANNNNTTC 1 cut(s) 911
PfeI GAWTC 6 cut(s) 4, 442, 673, 697, 743, 912
PflFI GACNNNGTC 1 cut(s) 302
PfoI TCCNGGA 1 cut(s) 415
PleI GAGTC 2 cut(s) 209, 363
PpsI GAGTC 2 cut(s) 209, 363
PpuMI RGGWCCY 1 cut(s) 752
Psp5II RGGWCCY 1 cut(s) 752
Psp6I CCWGG 2 cut(s) 381, 1492
PspFI CCCAGC 1 cut(s) 623
PspGI CCWGG 2 cut(s) 381, 1492
PspN4I GGNNCC 7 cut(s) 234, 318, 414, 628, 753, 841, 1597
PspOMI GGGCCC 1 cut(s) 626
PspPI GGNCC 8 cut(s) 317, 344, 413, 626, 627, 752, 977, 1186
PspPPI RGGWCCY 1 cut(s) 752
PstI CTGCAG 1 cut(s) 655
PsyI GACNNNGTC 1 cut(s) 302
RsaI GTAC 3 cut(s) 1051, 1091, 1366
RsaNI GTAC 3 cut(s) 1050, 1090, 1365
RseI CAYNNNNRTG 1 cut(s) 305
SapI GCTCTTC 3 cut(s) 551, 1458, 1581
SaqAI TTAA 1 cut(s) 758
Sau3AI GATC 2 cut(s) 1294, 1402
Sau96I GGNCC 8 cut(s) 317, 344, 413, 626, 627, 752, 977, 1186
SchI GAGTC 2 cut(s) 209, 364
ScrFI CCNGG 4 cut(s) 328, 383, 417, 1494
SduI GDGCHC 5 cut(s) 40, 630, 1385, 1453, 1531
SfaNI GCATC 4 cut(s) 535, 564, 869, 1305
SfcI CTRYAG 1 cut(s) 651
SinI GGWCC 4 cut(s) 317, 413, 752, 977
SmiMI CAYNNNNRTG 1 cut(s) 305
SmlI CTYRAG 1 cut(s) 1376
SmoI CTYRAG 1 cut(s) 1376
SphI GCATGC 2 cut(s) 1195, 1576
Sse9I AATT 4 cut(s) 518, 590, 814, 969
SsiI CCGC 7 cut(s) 112, 177, 369, 843, 876, 1189, 1552
SspI AATATT 1 cut(s) 963
SspMI CTAG 1 cut(s) 387
StyD4I CCNGG 4 cut(s) 326, 381, 415, 1492
StyI CCWWGG 5 cut(s) 15, 595, 793, 1265, 1443
TaaI ACNGT 3 cut(s) 35, 134, 424
TaiI ACGT 2 cut(s) 1402, 1608
TaqI TCGA 2 cut(s) 676, 1531
TaqII GACCGA 1 cut(s) 1185
TasI AATT 4 cut(s) 518, 590, 814, 969
TatI WGTACW 1 cut(s) 1364
TauI GCSGC 3 cut(s) 845, 878, 1555
TfiI GAWTC 6 cut(s) 4, 442, 673, 697, 743, 912
Tru1I TTAA 1 cut(s) 758
Tru9I TTAA 1 cut(s) 758
TscAI CASTG 6 cut(s) 40, 243, 496, 784, 922, 1026
TseFI GTSAC 2 cut(s) 431, 1203
TseI GCWGC 8 cut(s) 114, 404, 523, 653, 1064, 1195, 1462, 1497
Tsp45I GTSAC 2 cut(s) 431, 1203
TspDTI ATGAA 7 cut(s) 17, 442, 455, 591, 735, 752, 1402
TspRI CASTG 6 cut(s) 40, 243, 496, 784, 922, 1026
Tth111I GACNNNGTC 1 cut(s) 302
VpaK11BI GGWCC 4 cut(s) 317, 413, 752, 977
XceI RCATGY 2 cut(s) 1195, 1576
XmnI GAANNNNTTC 1 cut(s) 911
XspI CTAG 1 cut(s) 387
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.