Rh6DG136800

MatE

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr6D
OG0016752
Physical Location & Seq
Reverse (-)
19553417 .. 19559180
5764 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh6DG136800.1

Sequence Viewer

Length: 1401 bp
ATGAATCTGAAAACCATGGCGCTCCATTCCCACACTACTCACCTCCAAACCCATAAGCGCTTATCTCACCCTTCAATCTTGTACAACCCATTTCACACCTCTCACCGCAGCTCTGTTCGTTACGCCGTTAAGCCGCGAAGTTTTGGTGCTTTGGTGGTGGCGAAAAGCGAGCGTGGCAATGGACGGCTGATAACGGCGTGCATTGCTGGCGGTAATGCTGACGCGTTTGAAGAGGAAGAGGAGGAAGTGGTGGTGGAGGTCGTGGGTTCGAAGAAGGAGGAGTTGGTGGAGCAAAGCCTATGGAAGCAGGTGAAGGACATTGTCATGTTTTCGGGTCCGGCCACCGGGTTGTGGATTTGTGGGCCGTTGATGAGTCTCATCTCCACCGCTGTTATCGGCCAGGCTAGCTCTACTGAGCTTGCTGCTTTGGGTCCGGGAACTGTCTTCTGTGATAACATGAATCTTTTGTTCATGTTCCTTTCGATTGCGACTTCTAATATGGTTGCCACTGCGCTTGCCAAAAGGGATAAAAAGGAACTGCAGCATCAGATATCTATCTTGCTCTTCGTTGGATTGACTTGTGGAGTTCTGATGCTCTTGTTTACACAATTCCTTGGTTCATGGGCGCTAACAGCTTTTGCAGGGCCCAAGAATGTGCATATTATACCTGCAGCAAGCAAATATGTTCAGATTCGGGGGTTAGCGTGGCCTGCGATTCTTTTTGGATTGGTTGCTCAAAGCGCAAGTCTTGGCATGAAAGATTCATTGGGACCTTTAAAAGCTTTGGTGGTTGCCAGTGTTGTGAATGGCCTTGGTCATGTAGTCCTATGCAACATGTTACACTATGGTATTACTGGTGCAGCATGGGCAACAATGGCATCGCAGATTGTTGCTGCTTGTATGATGATTGAAGCTCTGAACAAGAAAGGATTTAGTGCATTTTCCATCTCTGTTCCATCGCCTCGTGAATGTCTGCTAATATTTGGGATTGCAGGTCCAGTGTTTGTAACAATGTTCTCAAAGGTGGCCTTCTACTCATTGATGACATACTTTGCTACTGCTATGGGTACAAATATAGTTGCTGCTCATCAGGTCATGATCCAAATGTACGGGATGTGCGTGGTATGGGGTGAGCCTCTTTCTCAAACTGCACAATCGTTTATGCCCGAGTTATTGTACGGAGTTAACCGAAGTTTGGAGAAGGCCCGAATGCTGCTAAAGTCACTCGTAATTATTGGAGCTATTCTTGGATTGGCAATAGGGGTTATTGGGATGTCCGTTCCTTGGTTGTTCCCCAATATCTTTACATTTGACCTGAAAGTCATACAAGAGGTTGGTTTTTATTTCAGGAGTATAAACTTCATTTCTTTTCCTATGATTTTTAATCTGACAGGACTATAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
MatE

Protein Analysis

466

Amino Acids

50.52

Weight (kDa)

9.03

Isoelectric Point (pI)

42.72

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
MatE PF01554 120 - 269 2.3e-15 MatE
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0016752)

Species Orthologous Gene IDs
fragaria_vesca FvH4_2g10120 FvH4_2g10120 FvH4_2g10120 FvH4_2g10120
rosa_chinensis RchiOBHm_Chr6g0267191
rosa_laevigata RLG00000014020
rosa_multiflora Rmu_sc0000908.1_g000024 Rmu_ssc0000388.1_g000047
rosa_roxburghii Rroxscaffold_7G00201000
rosa_rugosa Rorug06G0030400
rosa_samantha Rh6BG152600 Rh6CG148500 Rh6DG136800
rosa_wichuraiana Rw6G013140

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 298
AasI GACNNNNNNGTC 1 cut(s) 1319
Acc36I ACCTGC 3 cut(s) 298, 676, 983
AccII CGCG 2 cut(s) 136, 224
AciI CCGC 4 cut(s) 106, 134, 210, 387
AclWI GGATC 1 cut(s) 1093
AcoI YGGCCR 2 cut(s) 339, 397
AfaI GTAC 4 cut(s) 83, 1069, 1109, 1178
AfeI AGCGCT 1 cut(s) 59
AfiI CCNNNNNNNGG 4 cut(s) 344, 351, 1195, 1284
AflIII ACRYGT 2 cut(s) 222, 834
AgsI TTSAA 3 cut(s) 75, 230, 911
AjnI CCWGG 1 cut(s) 399
AjuI GAANNNNNNNTTGG 4 cut(s) 266, 298, 749, 781
AluBI AGCT 7 cut(s) 111, 408, 418, 635, 782, 914, 1241
AluI AGCT 7 cut(s) 111, 408, 418, 635, 782, 914, 1241
Alw26I GTCTC 1 cut(s) 380
AlwI GGATC 1 cut(s) 1093
Ama87I CYCGRG 1 cut(s) 1166
Aor51HI AGCGCT 1 cut(s) 59
AoxI GGCC 8 cut(s) 339, 362, 397, 644, 707, 808, 1026, 1203
ApaI GGGCCC 1 cut(s) 648
ApeKI GCWGC 8 cut(s) 108, 422, 541, 671, 860, 893, 1082, 1213
ArsI GACNNNNNNTTYG 2 cut(s) 730, 762
AspLEI GCGC 5 cut(s) 22, 60, 514, 628, 743
AspS9I GGNCC 8 cut(s) 335, 362, 431, 644, 645, 770, 995, 1204
AsuC2I CCSGG 2 cut(s) 346, 435
AsuHPI GGTGA 5 cut(s) 32, 59, 95, 322, 1142
AsuII TTCGAA 1 cut(s) 269
AsuNHI GCTAGC 1 cut(s) 404
AvaI CYCGRG 1 cut(s) 1166
AvaII GGWCC 4 cut(s) 335, 431, 770, 995
BaeGI GKGCMC 1 cut(s) 648
BanII GRGCYC 1 cut(s) 648
BauI CACGAG 1 cut(s) 963
BbsI GAAGAC 1 cut(s) 436
BbvI GCAGC 8 cut(s) 120, 409, 553, 683, 872, 880, 1069, 1200
BccI CCATC 2 cut(s) 953, 964
BceAI ACGGC 4 cut(s) 110, 200, 210, 349
BciT130I CCWGG 1 cut(s) 401
BcnI CCSGG 2 cut(s) 346, 435
BcoDI GTCTC 1 cut(s) 380
BfaI CTAG 1 cut(s) 405
BfmI CTRYAG 3 cut(s) 539, 669, 1397
BfoI RGCGCY 3 cut(s) 23, 61, 629
BfuAI ACCTGC 3 cut(s) 298, 676, 983
BisI GCNGC 9 cut(s) 109, 134, 423, 542, 672, 861, 894, 1083, 1214
BlsI GCNGC 9 cut(s) 110, 135, 424, 543, 673, 862, 895, 1084, 1215
Bme1390I CCNGG 3 cut(s) 346, 401, 435
Bme18I GGWCC 4 cut(s) 335, 431, 770, 995
BmeT110I CYCGRG 1 cut(s) 1166
BmgT120I GGNCC 8 cut(s) 335, 362, 431, 644, 645, 770, 995, 1204
BmiI GGNNCC 4 cut(s) 336, 432, 646, 771
BmrFI CCNGG 3 cut(s) 346, 401, 435
BmsI GCATC 3 cut(s) 553, 582, 887
BmtI GCTAGC 1 cut(s) 408
BpiI GAAGAC 1 cut(s) 436
Bpu14I TTCGAA 1 cut(s) 269
BpuMI CCSGG 2 cut(s) 346, 435
BsaBI GATNNNNATC 1 cut(s) 554
BsaJI CCNNGG 4 cut(s) 15, 613, 811, 1283
Bsc4I CCNNNNNNNGG 4 cut(s) 344, 351, 1195, 1284
Bse1I ACTGG 3 cut(s) 795, 859, 998
Bse3DI GCAATG 2 cut(s) 184, 201
Bse8I GATNNNNATC 1 cut(s) 554
BseBI CCWGG 1 cut(s) 401
BseDI CCNNGG 4 cut(s) 15, 613, 811, 1283
BseGI GGATG 2 cut(s) 1119, 1278
BseJI GATNNNNATC 1 cut(s) 554
BseLI CCNNNNNNNGG 4 cut(s) 344, 351, 1195, 1284
BseMI GCAATG 2 cut(s) 184, 201
BseMII CTCAG 1 cut(s) 405
BseNI ACTGG 3 cut(s) 795, 859, 998
BseRI GAGGAG 2 cut(s) 254, 293
BseSI GKGCMC 1 cut(s) 648
BseXI GCAGC 8 cut(s) 120, 409, 553, 683, 872, 880, 1069, 1200
BsgI GTGCAG 2 cut(s) 879, 1134
Bsh1236I CGCG 2 cut(s) 136, 224
BshFI GGCC 8 cut(s) 341, 364, 399, 646, 709, 810, 1028, 1205
BsiHKCI CYCGRG 1 cut(s) 1166
BsiSI CCGG 3 cut(s) 338, 345, 434
BslFI GGGAC 1 cut(s) 783
BslI CCNNNNNNNGG 4 cut(s) 344, 351, 1195, 1284
BsmAI GTCTC 1 cut(s) 380
BsmFI GGGAC 1 cut(s) 783
BsmI GAATGC 1 cut(s) 1215
BsnI GGCC 8 cut(s) 341, 364, 399, 646, 709, 810, 1028, 1205
BsoBI CYCGRG 1 cut(s) 1166
Bsp119I TTCGAA 1 cut(s) 269
Bsp120I GGGCCC 1 cut(s) 644
Bsp1286I GDGCHC 1 cut(s) 648
Bsp1407I TGTACA 1 cut(s) 81
Bsp143I GATC 1 cut(s) 1098
Bsp19I CCATGG 1 cut(s) 15
BspACI CCGC 4 cut(s) 106, 134, 210, 387
BspANI GGCC 8 cut(s) 341, 364, 399, 646, 709, 810, 1028, 1205
BspCNI CTCAG 1 cut(s) 406
BspFNI CGCG 2 cut(s) 136, 224
BspHI TCATGA 1 cut(s) 1095
BspLI GGNNCC 4 cut(s) 336, 432, 646, 771
BspMAI CTGCAG 2 cut(s) 543, 673
BspMI ACCTGC 3 cut(s) 298, 676, 983
BspOI GCTAGC 1 cut(s) 408
BspPI GGATC 1 cut(s) 1093
BspQI GCTCTTC 1 cut(s) 569
BspT104I TTCGAA 1 cut(s) 269
BsrDI GCAATG 2 cut(s) 184, 201
BsrGI TGTACA 1 cut(s) 81
BsrI ACTGG 3 cut(s) 795, 859, 998
BssECI CCNNGG 4 cut(s) 15, 613, 811, 1283
BssMI GATC 1 cut(s) 1098
BssSI CACGAG 1 cut(s) 963
BssT1I CCWWGG 4 cut(s) 15, 613, 811, 1283
Bst2BI CACGAG 1 cut(s) 963
Bst2UI CCWGG 1 cut(s) 401
Bst4CI ACNGT 1 cut(s) 442
Bst6I CTCTTC 3 cut(s) 225, 231, 569
BstAUI TGTACA 1 cut(s) 81
BstBI TTCGAA 1 cut(s) 269
BstC8I GCNNGC 8 cut(s) 170, 199, 208, 406, 420, 516, 676, 711
BstDEI CTNAG 1 cut(s) 414
BstDSI CCRYGG 1 cut(s) 15
BstF5I GGATG 2 cut(s) 1119, 1278
BstFNI CGCG 2 cut(s) 136, 224
BstH2I RGCGCY 3 cut(s) 23, 61, 629
BstHHI GCGC 5 cut(s) 22, 60, 514, 628, 743
BstKTI GATC 1 cut(s) 1101
BstMAI GTCTC 1 cut(s) 380
BstMBI GATC 1 cut(s) 1098
BstMWI GCNNNNNNNGC 9 cut(s) 174, 203, 207, 405, 632, 710, 740, 866, 875
BstNI CCWGG 1 cut(s) 401
BstNSI RCATGY 1 cut(s) 838
BstSCI CCNGG 3 cut(s) 344, 399, 433
BstSFI CTRYAG 3 cut(s) 539, 669, 1397
BstSLI GKGCMC 1 cut(s) 648
BstUI CGCG 2 cut(s) 136, 224
BstV1I GCAGC 8 cut(s) 120, 409, 553, 683, 872, 880, 1069, 1200
BstV2I GAAGAC 1 cut(s) 436
BsuRI GGCC 8 cut(s) 341, 364, 399, 646, 709, 810, 1028, 1205
BtgI CCRYGG 1 cut(s) 15
BtgZI GCGATG 2 cut(s) 864, 942
BtsCI GGATG 2 cut(s) 1119, 1278
BtsI GCAGTG 1 cut(s) 507
BtsIMutI CAGTG 3 cut(s) 507, 802, 1005
BveI ACCTGC 3 cut(s) 298, 676, 983
Cac8I GCNNGC 8 cut(s) 170, 199, 208, 406, 420, 516, 676, 711
CciI TCATGA 1 cut(s) 1095
CfoI GCGC 5 cut(s) 22, 60, 514, 628, 743
Cfr13I GGNCC 8 cut(s) 335, 362, 431, 644, 645, 770, 995, 1204
CseI GACGC 1 cut(s) 230
Csp6I GTAC 4 cut(s) 82, 1068, 1108, 1177
CspCI CAANNNNNGTGG 2 cut(s) 496, 531
CviQI GTAC 4 cut(s) 82, 1068, 1108, 1177
DdeI CTNAG 1 cut(s) 414
DpnI GATC 1 cut(s) 1100
DpnII GATC 1 cut(s) 1098
DraI TTTAAA 1 cut(s) 777
DrdI GACNNNNNNGTC 1 cut(s) 1319
DseDI GACNNNNNNGTC 1 cut(s) 1319
EaeI YGGCCR 2 cut(s) 339, 397
Eam1104I CTCTTC 3 cut(s) 225, 231, 569
EarI CTCTTC 3 cut(s) 225, 231, 569
Eco130I CCWWGG 4 cut(s) 15, 613, 811, 1283
Eco24I GRGCYC 1 cut(s) 648
Eco32I GATATC 1 cut(s) 552
Eco47I GGWCC 4 cut(s) 335, 431, 770, 995
Eco47III AGCGCT 1 cut(s) 59
Eco88I CYCGRG 1 cut(s) 1166
EcoO109I RGGNCCY 2 cut(s) 644, 770
EcoRII CCWGG 1 cut(s) 399
EcoRV GATATC 1 cut(s) 552
EcoT14I CCWWGG 4 cut(s) 15, 613, 811, 1283
EcoT38I GRGCYC 1 cut(s) 648
ErhI CCWWGG 4 cut(s) 15, 613, 811, 1283
FalI AAGNNNNNCTT 2 cut(s) 1013, 1045
FaqI GGGAC 1 cut(s) 783
Fnu4HI GCNGC 9 cut(s) 109, 134, 423, 542, 672, 861, 894, 1083, 1214
FokI GGATG 2 cut(s) 1126, 1285
FriOI GRGCYC 1 cut(s) 648
Fsp4HI GCNGC 9 cut(s) 109, 134, 423, 542, 672, 861, 894, 1083, 1214
FspBI CTAG 1 cut(s) 405
GlaI GCGC 5 cut(s) 21, 59, 513, 627, 742
GluI GCNGC 9 cut(s) 109, 134, 423, 542, 672, 861, 894, 1083, 1214
HaeII RGCGCY 3 cut(s) 23, 61, 629
HaeIII GGCC 8 cut(s) 341, 364, 399, 646, 709, 810, 1028, 1205
HapII CCGG 3 cut(s) 338, 345, 434
HgaI GACGC 1 cut(s) 230
HhaI GCGC 5 cut(s) 22, 60, 514, 628, 743
Hin6I GCGC 5 cut(s) 20, 58, 512, 626, 741
HinP1I GCGC 5 cut(s) 20, 58, 512, 626, 741
HincII GTYRAC 1 cut(s) 1186
HindII GTYRAC 1 cut(s) 1186
HindIII AAGCTT 1 cut(s) 780
HinfI GANTC 6 cut(s) 4, 373, 460, 691, 715, 761
HpaI GTTAAC 1 cut(s) 1186
HpaII CCGG 3 cut(s) 338, 345, 434
HphI GGTGA 5 cut(s) 32, 59, 95, 322, 1142
Hpy166II GTNNAC 2 cut(s) 603, 1186
Hpy188I TCNGA 6 cut(s) 9, 549, 591, 690, 918, 1389
Hpy188III TCNNGA 3 cut(s) 965, 1096, 1348
Hpy8I GTNNAC 2 cut(s) 603, 1186
HpyAV CCTTC 5 cut(s) 81, 268, 307, 1039, 1195
HpyCH4III ACNGT 1 cut(s) 442
HpyF10VI GCNNNNNNNGC 9 cut(s) 174, 203, 207, 405, 632, 710, 740, 866, 875
HpyF3I CTNAG 1 cut(s) 414
HspAI GCGC 5 cut(s) 20, 58, 512, 626, 741
KspAI GTTAAC 1 cut(s) 1186
Kzo9I GATC 1 cut(s) 1098
LguI GCTCTTC 1 cut(s) 569
LmnI GCTCC 3 cut(s) 27, 289, 1238
Lsp1109I GCAGC 8 cut(s) 120, 409, 553, 683, 872, 880, 1069, 1200
LweI GCATC 3 cut(s) 553, 582, 887
MaeI CTAG 1 cut(s) 405
MaeIII GTNAC 4 cut(s) 119, 837, 1006, 1221
MalI GATC 1 cut(s) 1100
MboI GATC 1 cut(s) 1098
MboII GAAGA 5 cut(s) 242, 248, 283, 436, 556
MhlI GDGCHC 1 cut(s) 648
MluCI AATT 2 cut(s) 608, 1230
MluI ACGCGT 1 cut(s) 222
MlyI GAGTC 1 cut(s) 382
MmeI TCCRAC 1 cut(s) 550
MseI TTAA 4 cut(s) 129, 776, 1185, 1383
MslI CAYNNNNRTG 1 cut(s) 323
MspA1I CMGCKG 1 cut(s) 389
MspI CCGG 3 cut(s) 338, 345, 434
MspR9I CCNGG 3 cut(s) 346, 401, 435
Mva1269I GAATGC 1 cut(s) 1215
MvaI CCWGG 1 cut(s) 401
MvnI CGCG 2 cut(s) 136, 224
MwoI GCNNNNNNNGC 9 cut(s) 174, 203, 207, 405, 632, 710, 740, 866, 875
NciI CCSGG 2 cut(s) 346, 435
NcoI CCATGG 1 cut(s) 15
NdeII GATC 1 cut(s) 1098
NheI GCTAGC 1 cut(s) 404
NlaIV GGNNCC 4 cut(s) 336, 432, 646, 771
NmuCI GTSAC 1 cut(s) 1221
NspI RCATGY 1 cut(s) 838
NspV TTCGAA 1 cut(s) 269
PagI TCATGA 1 cut(s) 1095
PaqCI CACCTGC 1 cut(s) 298
PciI ACATGT 1 cut(s) 834
PciSI GCTCTTC 1 cut(s) 569
PcsI WCGNNNNNNNCGW 1 cut(s) 1116
PctI GAATGC 1 cut(s) 1215
PfeI GAWTC 5 cut(s) 4, 460, 691, 715, 761
PflFI GACNNNGTC 1 cut(s) 320
PfoI TCCNGGA 1 cut(s) 433
PkrI GCNGC 9 cut(s) 110, 135, 424, 543, 673, 862, 895, 1084, 1215
PleI GAGTC 1 cut(s) 381
PpsI GAGTC 1 cut(s) 381
PpuMI RGGWCCY 1 cut(s) 770
PscI ACATGT 1 cut(s) 834
Psp5II RGGWCCY 1 cut(s) 770
Psp6I CCWGG 1 cut(s) 399
PspGI CCWGG 1 cut(s) 399
PspN4I GGNNCC 4 cut(s) 336, 432, 646, 771
PspOMI GGGCCC 1 cut(s) 644
PspPI GGNCC 8 cut(s) 335, 362, 431, 644, 645, 770, 995, 1204
PspPPI RGGWCCY 1 cut(s) 770
PstI CTGCAG 2 cut(s) 543, 673
PsyI GACNNNGTC 1 cut(s) 320
RsaI GTAC 4 cut(s) 83, 1069, 1109, 1178
RsaNI GTAC 4 cut(s) 82, 1068, 1108, 1177
RseI CAYNNNNRTG 1 cut(s) 323
SapI GCTCTTC 1 cut(s) 569
SaqAI TTAA 4 cut(s) 129, 776, 1185, 1383
SatI GCNGC 9 cut(s) 109, 134, 423, 542, 672, 861, 894, 1083, 1214
Sau3AI GATC 1 cut(s) 1098
Sau96I GGNCC 8 cut(s) 335, 362, 431, 644, 645, 770, 995, 1204
SchI GAGTC 1 cut(s) 382
ScrFI CCNGG 3 cut(s) 346, 401, 435
SduI GDGCHC 1 cut(s) 648
SfaNI GCATC 3 cut(s) 553, 582, 887
SfcI CTRYAG 3 cut(s) 539, 669, 1397
SfuI TTCGAA 1 cut(s) 269
SinI GGWCC 4 cut(s) 335, 431, 770, 995
SmiMI CAYNNNNRTG 1 cut(s) 323
Sse9I AATT 2 cut(s) 608, 1230
SsiI CCGC 4 cut(s) 106, 134, 210, 387
SspI AATATT 1 cut(s) 981
SspMI CTAG 1 cut(s) 405
StyD4I CCNGG 3 cut(s) 344, 399, 433
StyI CCWWGG 4 cut(s) 15, 613, 811, 1283
TaaI ACNGT 1 cut(s) 442
TaqI TCGA 2 cut(s) 269, 482
TasI AATT 2 cut(s) 608, 1230
TatI WGTACW 1 cut(s) 81
TauI GCSGC 1 cut(s) 136
TfiI GAWTC 5 cut(s) 4, 460, 691, 715, 761
Tru1I TTAA 4 cut(s) 129, 776, 1185, 1383
Tru9I TTAA 4 cut(s) 129, 776, 1185, 1383
TscAI CASTG 3 cut(s) 514, 802, 1005
TseFI GTSAC 1 cut(s) 1221
TseI GCWGC 8 cut(s) 108, 422, 541, 671, 860, 893, 1082, 1213
Tsp45I GTSAC 1 cut(s) 1221
TspDTI ATGAA 7 cut(s) 17, 460, 473, 609, 753, 770, 1351
TspGWI ACGGA 2 cut(s) 1194, 1267
TspRI CASTG 3 cut(s) 514, 802, 1005
Tth111I GACNNNGTC 1 cut(s) 320
VpaK11BI GGWCC 4 cut(s) 335, 431, 770, 995
XceI RCATGY 1 cut(s) 838
XspI CTAG 1 cut(s) 405
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.