Rroxscaffold_7G00201000

MatE

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000007
OG0016752
Physical Location & Seq
Forward (+)
48482053 .. 48487321
5269 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_7G00201000.1

Sequence Viewer

Length: 1362 bp
ATGGCGCTCCATTCCCACACTACTCACCTCCAAACCCATAGGCGCTTATCTCACCCTTCAATCCTGTACAACCCATTTCAGAACTCTCACCGCAGCTCTGTTCGTTACGCCGTTAAGCCGCGAAGTTTTGGTGCTTTGGTGGCGAAAAGCGAGCGTGGCAATGGACGGCTGATAACGGCGTGCATTGCTGGCGGTAATGCTGACGCATTTGAAGAGGAAGAGGAAGAGGAGCAAGTGGTGGTGGAGGTCGTGGGTTCGAAGAAGGAGGAGTTGGTGGAGCAAAGCCTGTGGAAGCAGGTGAAGGACATTGTCATGTTTTCGGGTCCGGCCACCGGGTTGTGGATTTGTGGGCCGTTGATGAGTCTCATCTCCACCGCTGTTATCGGCCAGGCAAGCTCTACTGAGCTTGCTGCTTTGGGTCCGGGAACAGTCTTCTGTGATAACATGAATCTTTTGTTCATGTTCCTTTCGATTGCGACTTCTAATATGGTTGCCACTGCGCTTGCCAAAAGGGATAAAAAGGAACTGCAGCATCAGATATCTATCTTGCTCTTCATTGGATTGACTTGTGGAGTTCTGATGCTCTTGTTTACACAATTCCTTGGTTCATGGGCTCTAACAGCTTTTGCTGGGCCCAAGAATGTGCATATTATACCTGCAGCAAGCAAATATGTTCAGATTGTTGCTGCTTGTATGATGATTGAAGCTCTGAACAAGAAAGGATTTAGTGCATTTTCCATCTCTGTTCCATCGCCTCGTGAATGTCTGCTAATATTTGGGATTGCAGGTCCAGTGTTTGTAACAATGTTCTCAAAGGTGGCCTTCTACTCACTGATGACATACTTTGCTACTGCTATGGGTACAAATATAGTTGCTGCTCATCAGGTCATGATCCAAATGTACGGGATGTGCGTGGTATGGGGTGAGCCTCTTTCTCAAACTGCACAATCGTTTATGCCCGAGTTATTGTACGGAGTTAACCGAAGTTTGGAGAAGGCCCGAATGCTGCTAAAGTCACTCGTAATTATTGGAGCTATTCTTGGATTGGCAATAGGGGTTATTGGGATGTCTGTTCCTTGGTTGTTCCCCAATATCTTTACATTTGACCTGAAAGTCATACAAGAGGCTGGACGTGATCTTACCTTCATCAGCTTGTCAATGAGCAGTTGTTTTTCCTTGGGTGCTCTTCTACTGTTGCTTGTGAACAGCAGAGGATTTGGTTTGCCAGGCTGCTGGTTTGCGCTTGTTGGCTTTCAATGGGCTCGATTTTTCCTTGCTCTCCGACGGCTTCTTTCTCCTAATGGCATGCTCCATTCAGAAGATCTGGGCTCGTATAAAGTGGGCAAGTTGAAAGCTACTTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
MatE

Protein Analysis

453

Amino Acids

49.44

Weight (kDa)

9.0

Isoelectric Point (pI)

46.94

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
MatE PF01554 116 - 238 2.5e-12 MatE
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0016752)

Species Orthologous Gene IDs
fragaria_vesca FvH4_2g10120 FvH4_2g10120 FvH4_2g10120 FvH4_2g10120
rosa_chinensis RchiOBHm_Chr6g0267191
rosa_laevigata RLG00000014020
rosa_multiflora Rmu_sc0000908.1_g000024 Rmu_ssc0000388.1_g000047
rosa_roxburghii Rroxscaffold_7G00201000
rosa_rugosa Rorug06G0030400
rosa_samantha Rh6BG152600 Rh6CG148500 Rh6DG136800
rosa_wichuraiana Rw6G013140

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 286
AasI GACNNNNNNGTC 1 cut(s) 1112
Acc36I ACCTGC 3 cut(s) 286, 664, 776
AccII CGCG 1 cut(s) 121
AciI CCGC 4 cut(s) 91, 119, 192, 375
AclWI GGATC 1 cut(s) 886
AcoI YGGCCR 2 cut(s) 327, 385
AfaI GTAC 4 cut(s) 68, 862, 902, 971
AfiI CCNNNNNNNGG 3 cut(s) 332, 339, 988
AgsI TTSAA 5 cut(s) 60, 212, 704, 1256, 1351
AjiI CACGTC 1 cut(s) 1133
AjnI CCWGG 2 cut(s) 387, 1225
AjuI GAANNNNNNNTTGG 2 cut(s) 254, 286
AluBI AGCT 8 cut(s) 96, 396, 406, 623, 707, 1034, 1152, 1355
AluI AGCT 8 cut(s) 96, 396, 406, 623, 707, 1034, 1152, 1355
Alw21I GWGCWC 1 cut(s) 1186
Alw26I GTCTC 1 cut(s) 368
AlwI GGATC 1 cut(s) 886
Ama87I CYCGRG 1 cut(s) 959
AoxI GGCC 6 cut(s) 327, 350, 385, 632, 819, 996
ApaI GGGCCC 1 cut(s) 636
ApeKI GCWGC 8 cut(s) 93, 410, 529, 659, 686, 875, 1006, 1230
AspLEI GCGC 4 cut(s) 7, 45, 502, 1243
AspS9I GGNCC 7 cut(s) 323, 350, 419, 632, 633, 788, 997
AsuC2I CCSGG 2 cut(s) 334, 423
AsuHPI GGTGA 5 cut(s) 17, 44, 80, 310, 935
AsuII TTCGAA 1 cut(s) 257
AvaI CYCGRG 1 cut(s) 959
AvaII GGWCC 3 cut(s) 323, 419, 788
BaeGI GKGCMC 1 cut(s) 636
BanII GRGCYC 4 cut(s) 616, 636, 1264, 1331
BauI CACGAG 1 cut(s) 756
BbsI GAAGAC 1 cut(s) 424
Bbv12I GWGCWC 1 cut(s) 1186
BbvI GCAGC 8 cut(s) 105, 397, 541, 671, 673, 862, 993, 1217
BccI CCATC 2 cut(s) 746, 757
BceAI ACGGC 5 cut(s) 95, 182, 192, 337, 1301
BciT130I CCWGG 2 cut(s) 389, 1227
BcnI CCSGG 2 cut(s) 334, 423
BcoDI GTCTC 1 cut(s) 368
BfmI CTRYAG 2 cut(s) 527, 657
BfoI RGCGCY 2 cut(s) 8, 46
BfuAI ACCTGC 3 cut(s) 286, 664, 776
BglII AGATCT 1 cut(s) 1321
BisI GCNGC 9 cut(s) 94, 119, 411, 530, 660, 687, 876, 1007, 1231
BlsI GCNGC 9 cut(s) 95, 120, 412, 531, 661, 688, 877, 1008, 1232
Bme1390I CCNGG 4 cut(s) 334, 389, 423, 1227
Bme18I GGWCC 3 cut(s) 323, 419, 788
BmeT110I CYCGRG 1 cut(s) 959
BmgBI CACGTC 1 cut(s) 1133
BmgT120I GGNCC 7 cut(s) 323, 350, 419, 632, 633, 788, 997
BmiI GGNNCC 3 cut(s) 324, 420, 634
BmrFI CCNGG 4 cut(s) 334, 389, 423, 1227
BmsI GCATC 2 cut(s) 541, 570
BpiI GAAGAC 1 cut(s) 424
Bpu14I TTCGAA 1 cut(s) 257
BpuMI CCSGG 2 cut(s) 334, 423
BsaBI GATNNNNATC 1 cut(s) 542
BsaJI CCNNGG 3 cut(s) 601, 1076, 1176
Bsc4I CCNNNNNNNGG 3 cut(s) 332, 339, 988
Bse1I ACTGG 1 cut(s) 791
Bse3DI GCAATG 2 cut(s) 166, 183
Bse8I GATNNNNATC 1 cut(s) 542
BseBI CCWGG 2 cut(s) 389, 1227
BseDI CCNNGG 3 cut(s) 601, 1076, 1176
BseGI GGATG 2 cut(s) 912, 1071
BseJI GATNNNNATC 1 cut(s) 542
BseLI CCNNNNNNNGG 3 cut(s) 332, 339, 988
BseMI GCAATG 2 cut(s) 166, 183
BseMII CTCAG 1 cut(s) 393
BseNI ACTGG 1 cut(s) 791
BseRI GAGGAG 2 cut(s) 242, 281
BseSI GKGCMC 1 cut(s) 636
BseXI GCAGC 8 cut(s) 105, 397, 541, 671, 673, 862, 993, 1217
BseYI CCCAGC 1 cut(s) 629
BsgI GTGCAG 1 cut(s) 927
Bsh1236I CGCG 1 cut(s) 121
BshFI GGCC 6 cut(s) 329, 352, 387, 634, 821, 998
BsiHKAI GWGCWC 1 cut(s) 1186
BsiHKCI CYCGRG 1 cut(s) 959
BsiSI CCGG 3 cut(s) 326, 333, 422
BslI CCNNNNNNNGG 3 cut(s) 332, 339, 988
BsmAI GTCTC 1 cut(s) 368
BsmI GAATGC 1 cut(s) 1008
BsnI GGCC 6 cut(s) 329, 352, 387, 634, 821, 998
BsoBI CYCGRG 1 cut(s) 959
Bsp119I TTCGAA 1 cut(s) 257
Bsp120I GGGCCC 1 cut(s) 632
Bsp1286I GDGCHC 5 cut(s) 616, 636, 1186, 1264, 1331
Bsp1407I TGTACA 1 cut(s) 66
Bsp143I GATC 3 cut(s) 891, 1135, 1321
BspACI CCGC 4 cut(s) 91, 119, 192, 375
BspANI GGCC 6 cut(s) 329, 352, 387, 634, 821, 998
BspCNI CTCAG 1 cut(s) 394
BspFNI CGCG 1 cut(s) 121
BspHI TCATGA 1 cut(s) 888
BspLI GGNNCC 3 cut(s) 324, 420, 634
BspMAI CTGCAG 2 cut(s) 531, 661
BspMI ACCTGC 3 cut(s) 286, 664, 776
BspPI GGATC 1 cut(s) 886
BspQI GCTCTTC 2 cut(s) 557, 1191
BspT104I TTCGAA 1 cut(s) 257
BsrDI GCAATG 2 cut(s) 166, 183
BsrGI TGTACA 1 cut(s) 66
BsrI ACTGG 1 cut(s) 791
BssECI CCNNGG 3 cut(s) 601, 1076, 1176
BssMI GATC 3 cut(s) 891, 1135, 1321
BssSI CACGAG 1 cut(s) 756
BssT1I CCWWGG 3 cut(s) 601, 1076, 1176
Bst2BI CACGAG 1 cut(s) 756
Bst2UI CCWGG 2 cut(s) 389, 1227
Bst4CI ACNGT 2 cut(s) 430, 1194
Bst6I CTCTTC 5 cut(s) 207, 213, 219, 557, 1191
BstAUI TGTACA 1 cut(s) 66
BstBI TTCGAA 1 cut(s) 257
BstC8I GCNNGC 8 cut(s) 152, 181, 190, 394, 408, 504, 664, 1307
BstDEI CTNAG 1 cut(s) 402
BstF5I GGATG 2 cut(s) 912, 1071
BstFNI CGCG 1 cut(s) 121
BstH2I RGCGCY 2 cut(s) 8, 46
BstHHI GCGC 4 cut(s) 7, 45, 502, 1243
BstKTI GATC 3 cut(s) 894, 1138, 1324
BstMAI GTCTC 1 cut(s) 368
BstMBI GATC 3 cut(s) 891, 1135, 1321
BstMWI GCNNNNNNNGC 6 cut(s) 140, 156, 185, 189, 393, 620
BstNI CCWGG 2 cut(s) 389, 1227
BstNSI RCATGY 1 cut(s) 1309
BstSCI CCNGG 4 cut(s) 332, 387, 421, 1225
BstSFI CTRYAG 2 cut(s) 527, 657
BstSLI GKGCMC 1 cut(s) 636
BstUI CGCG 1 cut(s) 121
BstV1I GCAGC 8 cut(s) 105, 397, 541, 671, 673, 862, 993, 1217
BstV2I GAAGAC 1 cut(s) 424
BstX2I RGATCY 1 cut(s) 1321
BstXI CCANNNNNNTGG 1 cut(s) 1233
BstYI RGATCY 1 cut(s) 1321
BsuRI GGCC 6 cut(s) 329, 352, 387, 634, 821, 998
BtgZI GCGATG 1 cut(s) 735
BtrI CACGTC 1 cut(s) 1133
BtsCI GGATG 2 cut(s) 912, 1071
BtsI GCAGTG 1 cut(s) 495
BtsIMutI CAGTG 3 cut(s) 495, 798, 830
BveI ACCTGC 3 cut(s) 286, 664, 776
Cac8I GCNNGC 8 cut(s) 152, 181, 190, 394, 408, 504, 664, 1307
CciI TCATGA 1 cut(s) 888
CfoI GCGC 4 cut(s) 7, 45, 502, 1243
Cfr13I GGNCC 7 cut(s) 323, 350, 419, 632, 633, 788, 997
CseI GACGC 1 cut(s) 212
Csp6I GTAC 4 cut(s) 67, 861, 901, 970
CspCI CAANNNNNGTGG 4 cut(s) 269, 304, 484, 519
CviAII CATG 6 cut(s) 313, 445, 460, 609, 889, 1306
CviQI GTAC 4 cut(s) 67, 861, 901, 970
DdeI CTNAG 1 cut(s) 402
DpnI GATC 3 cut(s) 893, 1137, 1323
DpnII GATC 3 cut(s) 891, 1135, 1321
DrdI GACNNNNNNGTC 1 cut(s) 1112
DseDI GACNNNNNNGTC 1 cut(s) 1112
EaeI YGGCCR 2 cut(s) 327, 385
Eam1104I CTCTTC 5 cut(s) 207, 213, 219, 557, 1191
EarI CTCTTC 5 cut(s) 207, 213, 219, 557, 1191
Eco130I CCWWGG 3 cut(s) 601, 1076, 1176
Eco24I GRGCYC 4 cut(s) 616, 636, 1264, 1331
Eco32I GATATC 1 cut(s) 540
Eco47I GGWCC 3 cut(s) 323, 419, 788
Eco88I CYCGRG 1 cut(s) 959
EcoRII CCWGG 2 cut(s) 387, 1225
EcoRV GATATC 1 cut(s) 540
EcoT14I CCWWGG 3 cut(s) 601, 1076, 1176
EcoT38I GRGCYC 4 cut(s) 616, 636, 1264, 1331
ErhI CCWWGG 3 cut(s) 601, 1076, 1176
FaeI CATG 6 cut(s) 316, 448, 463, 612, 892, 1309
FalI AAGNNNNNCTT 2 cut(s) 806, 838
FatI CATG 6 cut(s) 312, 444, 459, 608, 888, 1305
Fnu4HI GCNGC 9 cut(s) 94, 119, 411, 530, 660, 687, 876, 1007, 1231
FokI GGATG 2 cut(s) 919, 1078
FriOI GRGCYC 4 cut(s) 616, 636, 1264, 1331
Fsp4HI GCNGC 9 cut(s) 94, 119, 411, 530, 660, 687, 876, 1007, 1231
GlaI GCGC 4 cut(s) 6, 44, 501, 1242
GluI GCNGC 9 cut(s) 94, 119, 411, 530, 660, 687, 876, 1007, 1231
GsaI CCCAGC 1 cut(s) 633
HaeII RGCGCY 2 cut(s) 8, 46
HaeIII GGCC 6 cut(s) 329, 352, 387, 634, 821, 998
HapII CCGG 3 cut(s) 326, 333, 422
HgaI GACGC 1 cut(s) 212
HhaI GCGC 4 cut(s) 7, 45, 502, 1243
Hin1II CATG 6 cut(s) 316, 448, 463, 612, 892, 1309
Hin6I GCGC 4 cut(s) 5, 43, 500, 1241
HinP1I GCGC 4 cut(s) 5, 43, 500, 1241
HincII GTYRAC 1 cut(s) 979
HindII GTYRAC 1 cut(s) 979
HinfI GANTC 2 cut(s) 361, 448
HpaI GTTAAC 1 cut(s) 979
HpaII CCGG 3 cut(s) 326, 333, 422
HphI GGTGA 5 cut(s) 17, 44, 80, 310, 935
Hpy166II GTNNAC 3 cut(s) 591, 979, 1204
Hpy188I TCNGA 7 cut(s) 81, 537, 579, 678, 711, 1283, 1318
Hpy188III TCNNGA 2 cut(s) 758, 889
Hpy8I GTNNAC 3 cut(s) 591, 979, 1204
Hpy99I CGWCG 1 cut(s) 1287
HpyAV CCTTC 6 cut(s) 66, 256, 295, 832, 988, 1153
HpyCH4III ACNGT 2 cut(s) 430, 1194
HpyCH4IV ACGT 1 cut(s) 1132
HpyCH4V TGCA 7 cut(s) 183, 529, 646, 659, 731, 785, 944
HpyF10VI GCNNNNNNNGC 6 cut(s) 140, 156, 185, 189, 393, 620
HpyF3I CTNAG 1 cut(s) 402
HpySE526I ACGT 1 cut(s) 1132
Hsp92II CATG 6 cut(s) 316, 448, 463, 612, 892, 1309
HspAI GCGC 4 cut(s) 5, 43, 500, 1241
KspAI GTTAAC 1 cut(s) 979
Kzo9I GATC 3 cut(s) 891, 1135, 1321
LguI GCTCTTC 2 cut(s) 557, 1191
LmnI GCTCC 5 cut(s) 12, 229, 277, 1031, 1314
Lsp1109I GCAGC 8 cut(s) 105, 397, 541, 671, 673, 862, 993, 1217
LweI GCATC 2 cut(s) 541, 570
MaeII ACGT 1 cut(s) 1132
MaeIII GTNAC 3 cut(s) 104, 799, 1014
MalI GATC 3 cut(s) 893, 1137, 1323
MboI GATC 3 cut(s) 891, 1135, 1321
MboII GAAGA 8 cut(s) 224, 230, 236, 271, 424, 544, 1178, 1331
MflI RGATCY 1 cut(s) 1321
MhlI GDGCHC 5 cut(s) 616, 636, 1186, 1264, 1331
MluCI AATT 2 cut(s) 596, 1023
MlyI GAGTC 1 cut(s) 370
MmeI TCCRAC 1 cut(s) 1306
MseI TTAA 3 cut(s) 114, 978, 1360
MslI CAYNNNNRTG 1 cut(s) 311
MspA1I CMGCKG 1 cut(s) 377
MspI CCGG 3 cut(s) 326, 333, 422
MspR9I CCNGG 4 cut(s) 334, 389, 423, 1227
Mva1269I GAATGC 1 cut(s) 1008
MvaI CCWGG 2 cut(s) 389, 1227
MvnI CGCG 1 cut(s) 121
MwoI GCNNNNNNNGC 6 cut(s) 140, 156, 185, 189, 393, 620
NciI CCSGG 2 cut(s) 334, 423
NdeII GATC 3 cut(s) 891, 1135, 1321
NlaIII CATG 6 cut(s) 316, 448, 463, 612, 892, 1309
NlaIV GGNNCC 3 cut(s) 324, 420, 634
NmuCI GTSAC 1 cut(s) 1014
NspI RCATGY 1 cut(s) 1309
NspV TTCGAA 1 cut(s) 257
PaeI GCATGC 1 cut(s) 1309
PagI TCATGA 1 cut(s) 888
PaqCI CACCTGC 1 cut(s) 286
PciSI GCTCTTC 2 cut(s) 557, 1191
PcsI WCGNNNNNNNCGW 1 cut(s) 909
PctI GAATGC 1 cut(s) 1008
PfeI GAWTC 1 cut(s) 448
PflFI GACNNNGTC 1 cut(s) 308
PfoI TCCNGGA 1 cut(s) 421
PkrI GCNGC 9 cut(s) 95, 120, 412, 531, 661, 688, 877, 1008, 1232
PleI GAGTC 1 cut(s) 369
PpsI GAGTC 1 cut(s) 369
Psp6I CCWGG 2 cut(s) 387, 1225
PspFI CCCAGC 1 cut(s) 629
PspGI CCWGG 2 cut(s) 387, 1225
PspN4I GGNNCC 3 cut(s) 324, 420, 634
PspOMI GGGCCC 1 cut(s) 632
PspPI GGNCC 7 cut(s) 323, 350, 419, 632, 633, 788, 997
PstI CTGCAG 2 cut(s) 531, 661
PsuI RGATCY 1 cut(s) 1321
PsyI GACNNNGTC 1 cut(s) 308
RsaI GTAC 4 cut(s) 68, 862, 902, 971
RsaNI GTAC 4 cut(s) 67, 861, 901, 970
RseI CAYNNNNRTG 1 cut(s) 311
SapI GCTCTTC 2 cut(s) 557, 1191
SaqAI TTAA 3 cut(s) 114, 978, 1360
SatI GCNGC 9 cut(s) 94, 119, 411, 530, 660, 687, 876, 1007, 1231
Sau3AI GATC 3 cut(s) 891, 1135, 1321
Sau96I GGNCC 7 cut(s) 323, 350, 419, 632, 633, 788, 997
SchI GAGTC 1 cut(s) 370
ScrFI CCNGG 4 cut(s) 334, 389, 423, 1227
SduI GDGCHC 5 cut(s) 616, 636, 1186, 1264, 1331
SfaNI GCATC 2 cut(s) 541, 570
SfcI CTRYAG 2 cut(s) 527, 657
SfuI TTCGAA 1 cut(s) 257
SinI GGWCC 3 cut(s) 323, 419, 788
SmiMI CAYNNNNRTG 1 cut(s) 311
SphI GCATGC 1 cut(s) 1309
Sse9I AATT 2 cut(s) 596, 1023
SsiI CCGC 4 cut(s) 91, 119, 192, 375
SspI AATATT 1 cut(s) 774
StyD4I CCNGG 4 cut(s) 332, 387, 421, 1225
StyI CCWWGG 3 cut(s) 601, 1076, 1176
TaaI ACNGT 2 cut(s) 430, 1194
TaiI ACGT 1 cut(s) 1135
TaqI TCGA 3 cut(s) 257, 470, 1264
TasI AATT 2 cut(s) 596, 1023
TatI WGTACW 1 cut(s) 66
TauI GCSGC 1 cut(s) 121
TfiI GAWTC 1 cut(s) 448
Tru1I TTAA 3 cut(s) 114, 978, 1360
Tru9I TTAA 3 cut(s) 114, 978, 1360
TscAI CASTG 3 cut(s) 502, 798, 837
TseFI GTSAC 1 cut(s) 1014
TseI GCWGC 8 cut(s) 93, 410, 529, 659, 686, 875, 1006, 1230
Tsp45I GTSAC 1 cut(s) 1014
TspDTI ATGAA 5 cut(s) 448, 461, 544, 597, 1135
TspGWI ACGGA 1 cut(s) 987
TspRI CASTG 3 cut(s) 502, 798, 837
Tth111I GACNNNGTC 1 cut(s) 308
VpaK11BI GGWCC 3 cut(s) 323, 419, 788
XceI RCATGY 1 cut(s) 1309
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.