FvH4_2g13610

acyl-activating enzyme 1

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb2
OG0014332
Physical Location & Seq
Reverse (-)
11877359 .. 11881584
4226 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_2g13610.t1

Sequence Viewer

Length: 1665 bp
ATGGAGGGTGCAATTAGGTGCTCCGCCAACTATGTTCCTCTTTCGCCTATCAGCTTCTTGGAGCGCTCAGCCATAGTCTACAGAGACAGACCATCTGTTGTGTATGGAGACATCATCCACACTTGGAGAGAGACACTTGAACGATGCACCAGACTTGCTTCTGCTCTTGCCCAACTTGGAATTTCCCGAGGCGATGTGGTTGCTGCATTGGCTCCAAATATTCCAGCAATGTATGAGCTCCATTTTGGTGTTCCAATGGCTGGTGCAATTTTGTGCACACTTAACATACGTCATGATTCAGAAATGGTGTCAGTATTAATGAAGCATTCAGAAACCAAAATCATTTTTGTAGACCACCAATTTCTCCAAGTTGCAAAGGGAGCAATTGATATCTTATCCAAGACCGGAACCAAGGTGCCCCTCCTAGTCTTGATTCCAGAGAGTGATCAGTCATCCCCTGACTTCTCCAAATCCCCTTCAGCAAACTTAGAATATGAGAGCCTGTTAGGAAAGGGAAATCTCGATTTTCAGATCCTACGGCCGAAAGACGAATGGGATGCAATTTCGCTTAACTACACATCAGGCACTACTTCAAGCCCAAAAGGTGTCATTTATAGCCATAGAGGTGCATATATCAATTCCCTGTCTGCAGTTATTCTCAATGAGATGCTATCAATGCCTGTGTATCTATGGTGTGTTCCAATGTTTCATTGCAACGGATGGTGCCTCACTTGGGCTGTTGCGGCTCAGGGTGGCACTAATGTCTGCCAAAGAAATGTTACTGCAAAGGGAATTTTCGCCTGTATCTCTCAGCATCAGGTGACCCACATGGGTGGTGCACCTACTGTCTTGAACATGATTGTAAATGCACCGGAAAATGATCGGAGGCCACTTCCAGGAAAGGTAGTGGTCATGACTGGGGGTGCACCACCACCATCCCAGGTACTGTTCAAGATGGAAGAGCTAGGGTTCAGTGTTACACATTCATATGGTTTGACAGAGACTTATGGCCCTGGGACAGTTTGCTCTTGGAAACCTGAATGGAATTCTCTACCTCGAGATGAACAAGCAAAAATAAAGTCACGGCAAGGGTTGCAACATATCGGAATGGAGGAACTTGATGTTAAAGATCCGGTCACGATGAAGAGTGCCCCTTTTGATGCAAAAACCATGGGGGAGGTTATGTTTAGGGGAAACCCTGTTATGAATGGATATTTCAAAGATAATAAATCAACACAGGATGCATTTAAAGGTGGATGGTTTCGCAGTGGAGACTTGGCAGTCAGACACCCAGATGGTTATATAGAGCTAAAGGATCGTTCCAAGGATATTATAATTTCCGGGGGAGAAAATATTAGCACAATAGAGGTGGAATCAGTGCTTTTCAAGCATCCAGAGATTCTTGAGGCAGCTATCGTAGGAAGGCCTGATGAATATTGGGGGGAGACACCCTGTGCTTTTGTGAAATTGAAGGATGGTTGTAACGCTAGTGCAGAAGATATCATTAAGTTCTGTAGGGGTCGGTTACCCCGCTACATGGCTCCTCGAACTGTCATATTCGAAGATTTACCAAAGACTTCTACTGGGAAGGTGCAAAAGTTTGTACTGAGAGAGAAAGCTAAGGCCATGGGAAGTCTCTCCAAGAGTAGCATCAGCAAACTGTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005777 GO:0042579 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0044424 GO:0044444 GO:0044464
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
AMP-binding AMP-binding_C

Protein Analysis

555

Amino Acids

61.03

Weight (kDa)

7.9

Isoelectric Point (pI)

36.77

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
AMP-binding PF00501 20 - 406 7.9e-74 AMP-binding enzyme
AMP-binding_C PF13193 456 - 530 2.3e-21 AMP-binding enzyme C-terminal domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0014332)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G20560 AT1G20560
fragaria_vesca FvH4_2g13610 FvH4_2g13610 FvH4_2g13610 FvH4_2g13610
malus_domestica MD05G1027100.v1.1
prunus_persica Prupe.8G032600_v2.0.a1 Prupe.8G032600_v2.0.a1
pyrus_communis pycom05g01910
rosa_chinensis RchiOBHm_Chr6g0275221
rosa_laevigata RLG00000013486
rosa_roxburghii Rroxscaffold_7G00193540
rosa_rugosa Rorug06G0090300
rosa_samantha Rh6AG203600 Rh6CG209000
rosa_wichuraiana Rw6G017670

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 1334
AasI GACNNNNNNGTC 1 cut(s) 1280
AccB1I GGYRCC 2 cut(s) 415, 723
AccB7I CCANNNNNTGG 1 cut(s) 260
AccI GTMKAC 2 cut(s) 78, 351
AciI CCGC 3 cut(s) 24, 743, 1531
AclWI GGATC 3 cut(s) 526, 1124, 1323
AcoI YGGCCR 1 cut(s) 539
AcsI RAATTY 3 cut(s) 180, 792, 1045
AcuI CTGAAG 1 cut(s) 462
AdeI CACNNNGTG 1 cut(s) 1454
AfaI GTAC 2 cut(s) 945, 1605
AfeI AGCGCT 1 cut(s) 65
AfiI CCNNNNNNNGG 4 cut(s) 260, 733, 896, 1537
AgsI TTSAA 7 cut(s) 140, 594, 853, 952, 1219, 1387, 1471
AjnI CCWGG 3 cut(s) 895, 939, 1012
AleI CACNNNNGTG 1 cut(s) 831
AluBI AGCT 6 cut(s) 54, 238, 964, 1309, 1412, 1619
AluI AGCT 6 cut(s) 54, 238, 964, 1309, 1412, 1619
Alw21I GWGCWC 5 cut(s) 23, 240, 278, 841, 928
Alw26I GTCTC 7 cut(s) 78, 102, 125, 995, 1266, 1439, 1640
Alw44I GTGCAC 3 cut(s) 274, 837, 924
AlwI GGATC 3 cut(s) 526, 1124, 1323
AlwNI CAGNNNCTG 1 cut(s) 946
Ama87I CYCGRG 2 cut(s) 186, 1056
Aor51HI AGCGCT 1 cut(s) 65
AoxI GGCC 5 cut(s) 539, 887, 1009, 1424, 1623
ApaLI GTGCAC 3 cut(s) 274, 837, 924
ApeKI GCWGC 2 cut(s) 203, 1409
ApoI RAATTY 3 cut(s) 180, 792, 1045
ArsI GACNNNNNNTTYG 2 cut(s) 1064, 1096
AseI ATTAAT 1 cut(s) 317
AspLEI GCGC 1 cut(s) 66
AspS9I GGNCC 1 cut(s) 1010
AsuC2I CCSGG 1 cut(s) 1342
AsuHPI GGTGA 1 cut(s) 832
AsuII TTCGAA 1 cut(s) 1560
AvaI CYCGRG 2 cut(s) 186, 1056
BaeGI GKGCMC 5 cut(s) 278, 420, 841, 928, 1153
BanI GGYRCC 2 cut(s) 415, 723
BanII GRGCYC 1 cut(s) 240
Bbv12I GWGCWC 5 cut(s) 23, 240, 278, 841, 928
BbvI GCAGC 2 cut(s) 190, 1421
BccI CCATC 7 cut(s) 100, 714, 943, 949, 1251, 1289, 1469
BceAI ACGGC 2 cut(s) 554, 1100
BcgI CGANNNNNNTGC 4 cut(s) 182, 216, 539, 573
BciT130I CCWGG 3 cut(s) 897, 941, 1014
BclI TGATCA 1 cut(s) 445
BcnI CCSGG 1 cut(s) 1342
BcoDI GTCTC 7 cut(s) 78, 102, 125, 995, 1266, 1439, 1640
BfaI CTAG 3 cut(s) 425, 965, 1488
BfmI CTRYAG 3 cut(s) 79, 648, 1513
BfoI RGCGCY 1 cut(s) 67
BisI GCNGC 3 cut(s) 204, 744, 1410
BlpI GCTNAGC 1 cut(s) 67
BlsI GCNGC 3 cut(s) 205, 745, 1411
Bme1390I CCNGG 4 cut(s) 897, 941, 1014, 1342
BmeT110I CYCGRG 2 cut(s) 186, 1056
BmgT120I GGNCC 1 cut(s) 1010
BmiI GGNNCC 5 cut(s) 213, 409, 417, 725, 1542
BmrFI CCNGG 4 cut(s) 897, 941, 1014, 1342
BmrI ACTGGG 2 cut(s) 927, 1593
BmsI GCATC 8 cut(s) 134, 547, 657, 823, 1150, 1231, 1399, 1659
BmuI ACTGGG 2 cut(s) 927, 1593
Bpu10I CCTNAGC 2 cut(s) 747, 1620
Bpu1102I GCTNAGC 1 cut(s) 67
Bpu14I TTCGAA 1 cut(s) 1560
BpuEI CTTGAG 1 cut(s) 1424
BpuMI CCSGG 1 cut(s) 1342
BsaJI CCNNGG 9 cut(s) 187, 411, 939, 1012, 1013, 1170, 1323, 1341, 1626
BsaWI WCCGGW 3 cut(s) 404, 871, 1132
BsaXI ACNNNNNCTCC 2 cut(s) 1169, 1199
Bsc4I CCNNNNNNNGG 4 cut(s) 260, 733, 896, 1537
Bse1I ACTGG 2 cut(s) 922, 1588
Bse3DI GCAATG 2 cut(s) 234, 709
BseBI CCWGG 3 cut(s) 897, 941, 1014
BseDI CCNNGG 9 cut(s) 187, 411, 939, 1012, 1013, 1170, 1323, 1341, 1626
BseGI GGATG 9 cut(s) 114, 452, 562, 725, 935, 1246, 1262, 1390, 1480
BseLI CCNNNNNNNGG 4 cut(s) 260, 733, 896, 1537
BseMI GCAATG 2 cut(s) 234, 709
BseMII CTCAG 4 cut(s) 81, 761, 824, 1598
BseNI ACTGG 2 cut(s) 922, 1588
BseRI GAGGAG 1 cut(s) 1533
BseSI GKGCMC 5 cut(s) 278, 420, 841, 928, 1153
BseX3I CGGCCG 1 cut(s) 539
BseXI GCAGC 2 cut(s) 190, 1421
BsgI GTGCAG 1 cut(s) 1512
Bsh1285I CGRYCG 1 cut(s) 542
BshFI GGCC 5 cut(s) 541, 889, 1011, 1426, 1625
BshNI GGYRCC 2 cut(s) 415, 723
BsiEI CGRYCG 1 cut(s) 542
BsiHKAI GWGCWC 5 cut(s) 23, 240, 278, 841, 928
BsiHKCI CYCGRG 2 cut(s) 186, 1056
BsiSI CCGG 4 cut(s) 405, 872, 1133, 1341
BslFI GGGAC 1 cut(s) 1030
BslI CCNNNNNNNGG 4 cut(s) 260, 733, 896, 1537
BsmAI GTCTC 7 cut(s) 78, 102, 125, 995, 1266, 1439, 1640
BsmFI GGGAC 1 cut(s) 1030
BsmI GAATGC 1 cut(s) 325
BsnI GGCC 5 cut(s) 541, 889, 1011, 1426, 1625
BsoBI CYCGRG 2 cut(s) 186, 1056
Bsp119I TTCGAA 1 cut(s) 1560
Bsp1286I GDGCHC 7 cut(s) 23, 240, 278, 420, 841, 928, 1153
Bsp143I GATC 5 cut(s) 445, 531, 880, 1129, 1315
Bsp1720I GCTNAGC 1 cut(s) 67
Bsp19I CCATGG 2 cut(s) 1170, 1626
BspACI CCGC 3 cut(s) 24, 743, 1531
BspANI GGCC 5 cut(s) 541, 889, 1011, 1426, 1625
BspCNI CTCAG 4 cut(s) 80, 760, 823, 1599
BspHI TCATGA 2 cut(s) 292, 912
BspLI GGNNCC 5 cut(s) 213, 409, 417, 725, 1542
BspMAI CTGCAG 1 cut(s) 652
BspPI GGATC 3 cut(s) 526, 1124, 1323
BspQI GCTCTTC 1 cut(s) 954
BspT104I TTCGAA 1 cut(s) 1560
BspT107I GGYRCC 2 cut(s) 415, 723
BsrDI GCAATG 2 cut(s) 234, 709
BsrI ACTGG 2 cut(s) 922, 1588
BssECI CCNNGG 9 cut(s) 187, 411, 939, 1012, 1013, 1170, 1323, 1341, 1626
BssMI GATC 5 cut(s) 445, 531, 880, 1129, 1315
BssT1I CCWWGG 4 cut(s) 411, 1170, 1323, 1626
Bst2UI CCWGG 3 cut(s) 897, 941, 1014
Bst4CI ACNGT 5 cut(s) 847, 948, 1021, 1552, 1662
Bst6I CTCTTC 2 cut(s) 954, 1139
BstAPI GCANNNNNTGC 1 cut(s) 1093
BstBI TTCGAA 1 cut(s) 1560
BstDEI CTNAG 6 cut(s) 67, 487, 747, 810, 1607, 1620
BstDSI CCRYGG 2 cut(s) 1170, 1626
BstEII GGTNACC 2 cut(s) 820, 1524
BstF5I GGATG 9 cut(s) 114, 452, 562, 725, 935, 1246, 1262, 1390, 1480
BstH2I RGCGCY 1 cut(s) 67
BstHHI GCGC 1 cut(s) 66
BstKTI GATC 5 cut(s) 448, 534, 883, 1132, 1318
BstMAI GTCTC 7 cut(s) 78, 102, 125, 995, 1266, 1439, 1640
BstMBI GATC 5 cut(s) 445, 531, 880, 1129, 1315
BstMCI CGRYCG 1 cut(s) 542
BstMWI GCNNNNNNNGC 6 cut(s) 209, 380, 676, 743, 1093, 1387
BstNI CCWGG 3 cut(s) 897, 941, 1014
BstPI GGTNACC 2 cut(s) 820, 1524
BstSCI CCNGG 4 cut(s) 895, 939, 1012, 1340
BstSFI CTRYAG 3 cut(s) 79, 648, 1513
BstSLI GKGCMC 5 cut(s) 278, 420, 841, 928, 1153
BstV1I GCAGC 2 cut(s) 190, 1421
BstX2I RGATCY 2 cut(s) 531, 1129
BstXI CCANNNNNNTGG 1 cut(s) 833
BstYI RGATCY 2 cut(s) 531, 1129
BstZI CGGCCG 1 cut(s) 539
BsuRI GGCC 5 cut(s) 541, 889, 1011, 1426, 1625
BtgI CCRYGG 2 cut(s) 1170, 1626
BtgZI GCGATG 1 cut(s) 207
BtsCI GGATG 9 cut(s) 114, 452, 562, 725, 935, 1246, 1262, 1390, 1480
BtsI GCAGTG 1 cut(s) 1273
BtsIMutI CAGTG 3 cut(s) 979, 1273, 1383
CaiI CAGNNNCTG 1 cut(s) 946
CciI TCATGA 2 cut(s) 292, 912
CfoI GCGC 1 cut(s) 66
Cfr13I GGNCC 1 cut(s) 1010
Csp6I GTAC 2 cut(s) 944, 1604
CspCI CAANNNNNGTGG 2 cut(s) 1350, 1385
CviAII CATG 7 cut(s) 293, 829, 856, 913, 1171, 1537, 1627
CviQI GTAC 2 cut(s) 944, 1604
DdeI CTNAG 6 cut(s) 67, 487, 747, 810, 1607, 1620
DpnI GATC 5 cut(s) 447, 533, 882, 1131, 1317
DpnII GATC 5 cut(s) 445, 531, 880, 1129, 1315
DraI TTTAAA 1 cut(s) 1249
DraIII CACNNNGTG 1 cut(s) 1454
DrdI GACNNNNNNGTC 1 cut(s) 1280
DseDI GACNNNNNNGTC 1 cut(s) 1280
EaeI YGGCCR 1 cut(s) 539
EagI CGGCCG 1 cut(s) 539
Eam1104I CTCTTC 2 cut(s) 954, 1139
EarI CTCTTC 2 cut(s) 954, 1139
EciI GGCGGA 1 cut(s) 13
Ecl136II GAGCTC 1 cut(s) 238
EclXI CGGCCG 1 cut(s) 539
Eco130I CCWWGG 4 cut(s) 411, 1170, 1323, 1626
Eco147I AGGCCT 1 cut(s) 1426
Eco24I GRGCYC 1 cut(s) 240
Eco32I GATATC 2 cut(s) 391, 1501
Eco47III AGCGCT 1 cut(s) 65
Eco52I CGGCCG 1 cut(s) 539
Eco53kI GAGCTC 1 cut(s) 238
Eco57I CTGAAG 1 cut(s) 462
Eco88I CYCGRG 2 cut(s) 186, 1056
Eco91I GGTNACC 2 cut(s) 820, 1524
EcoICRI GAGCTC 1 cut(s) 238
EcoO65I GGTNACC 2 cut(s) 820, 1524
EcoRI GAATTC 1 cut(s) 1045
EcoRII CCWGG 3 cut(s) 895, 939, 1012
EcoRV GATATC 2 cut(s) 391, 1501
EcoT14I CCWWGG 4 cut(s) 411, 1170, 1323, 1626
EcoT22I ATGCAT 1 cut(s) 1246
EcoT38I GRGCYC 1 cut(s) 240
ErhI CCWWGG 4 cut(s) 411, 1170, 1323, 1626
FaeI CATG 7 cut(s) 296, 832, 859, 916, 1174, 1540, 1630
FaqI GGGAC 1 cut(s) 1030
FatI CATG 7 cut(s) 292, 828, 855, 912, 1170, 1536, 1626
FauI CCCGC 1 cut(s) 1538
FauNDI CATATG 1 cut(s) 988
FbaI TGATCA 1 cut(s) 445
FblI GTMKAC 2 cut(s) 78, 351
Fnu4HI GCNGC 3 cut(s) 204, 744, 1410
FokI GGATG 9 cut(s) 101, 439, 569, 732, 922, 1253, 1269, 1377, 1487
FriOI GRGCYC 1 cut(s) 240
Fsp4HI GCNGC 3 cut(s) 204, 744, 1410
FspBI CTAG 3 cut(s) 425, 965, 1488
GlaI GCGC 1 cut(s) 65
GluI GCNGC 3 cut(s) 204, 744, 1410
HaeII RGCGCY 1 cut(s) 67
HaeIII GGCC 5 cut(s) 541, 889, 1011, 1426, 1625
HapII CCGG 4 cut(s) 405, 872, 1133, 1341
HhaI GCGC 1 cut(s) 66
Hin1II CATG 7 cut(s) 296, 832, 859, 916, 1174, 1540, 1630
Hin6I GCGC 1 cut(s) 64
HinP1I GCGC 1 cut(s) 64
HinfI GANTC 4 cut(s) 296, 433, 1373, 1399
HpaII CCGG 4 cut(s) 405, 872, 1133, 1341
HphI GGTGA 1 cut(s) 832
Hpy166II GTNNAC 5 cut(s) 79, 276, 352, 839, 926
Hpy188I TCNGA 6 cut(s) 301, 331, 531, 885, 1106, 1286
Hpy8I GTNNAC 5 cut(s) 79, 276, 352, 839, 926
HpyAV CCTTC 4 cut(s) 486, 1416, 1465, 1582
HpyCH4III ACNGT 5 cut(s) 847, 948, 1021, 1552, 1662
HpyCH4IV ACGT 1 cut(s) 289
HpyF10VI GCNNNNNNNGC 6 cut(s) 209, 380, 676, 743, 1093, 1387
HpyF3I CTNAG 6 cut(s) 67, 487, 747, 810, 1607, 1620
HpySE526I ACGT 1 cut(s) 289
Hsp92II CATG 7 cut(s) 296, 832, 859, 916, 1174, 1540, 1630
HspAI GCGC 1 cut(s) 64
Ksp22I TGATCA 1 cut(s) 445
Kzo9I GATC 5 cut(s) 445, 531, 880, 1129, 1315
LguI GCTCTTC 1 cut(s) 954
LmnI GCTCC 6 cut(s) 26, 61, 217, 243, 380, 1546
Lsp1109I GCAGC 2 cut(s) 190, 1421
LweI GCATC 8 cut(s) 134, 547, 657, 823, 1150, 1231, 1399, 1659
MaeI CTAG 3 cut(s) 425, 965, 1488
MaeII ACGT 1 cut(s) 289
MaeIII GTNAC 7 cut(s) 778, 820, 976, 1080, 1135, 1481, 1524
MalI GATC 5 cut(s) 447, 533, 882, 1131, 1317
MboI GATC 5 cut(s) 445, 531, 880, 1129, 1315
MboII GAAGA 4 cut(s) 971, 1156, 1508, 1574
MfeI CAATTG 1 cut(s) 384
MflI RGATCY 2 cut(s) 531, 1129
MhlI GDGCHC 7 cut(s) 23, 240, 278, 420, 841, 928, 1153
Mph1103I ATGCAT 1 cut(s) 1246
MseI TTAA 6 cut(s) 282, 317, 570, 1125, 1248, 1506
MslI CAYNNNNRTG 5 cut(s) 246, 624, 831, 987, 1293
MspI CCGG 4 cut(s) 405, 872, 1133, 1341
MspR9I CCNGG 4 cut(s) 897, 941, 1014, 1342
MunI CAATTG 1 cut(s) 384
Mva1269I GAATGC 1 cut(s) 325
MvaI CCWGG 3 cut(s) 897, 941, 1014
MwoI GCNNNNNNNGC 6 cut(s) 209, 380, 676, 743, 1093, 1387
NciI CCSGG 1 cut(s) 1342
NcoI CCATGG 2 cut(s) 1170, 1626
NdeI CATATG 1 cut(s) 988
NdeII GATC 5 cut(s) 445, 531, 880, 1129, 1315
NlaIII CATG 7 cut(s) 296, 832, 859, 916, 1174, 1540, 1630
NlaIV GGNNCC 5 cut(s) 213, 409, 417, 725, 1542
NmuCI GTSAC 3 cut(s) 820, 1080, 1135
NsiI ATGCAT 1 cut(s) 1246
NspV TTCGAA 1 cut(s) 1560
OliI CACNNNNGTG 1 cut(s) 831
PaeR7I CTCGAG 1 cut(s) 1056
PagI TCATGA 2 cut(s) 292, 912
PasI CCCWGGG 1 cut(s) 1013
PceI AGGCCT 1 cut(s) 1426
PciSI GCTCTTC 1 cut(s) 954
PctI GAATGC 1 cut(s) 325
PfeI GAWTC 4 cut(s) 296, 433, 1373, 1399
PflMI CCANNNNNTGG 1 cut(s) 260
PfoI TCCNGGA 1 cut(s) 895
PkrI GCNGC 3 cut(s) 205, 745, 1411
PshBI ATTAAT 1 cut(s) 317
PsiI TTATAA 1 cut(s) 1334
Psp124BI GAGCTC 1 cut(s) 240
Psp6I CCWGG 3 cut(s) 895, 939, 1012
PspEI GGTNACC 2 cut(s) 820, 1524
PspGI CCWGG 3 cut(s) 895, 939, 1012
PspN4I GGNNCC 5 cut(s) 213, 409, 417, 725, 1542
PspPI GGNCC 1 cut(s) 1010
PstI CTGCAG 1 cut(s) 652
PstNI CAGNNNCTG 1 cut(s) 946
PsuI RGATCY 2 cut(s) 531, 1129
RsaI GTAC 2 cut(s) 945, 1605
RsaNI GTAC 2 cut(s) 944, 1604
RseI CAYNNNNRTG 5 cut(s) 246, 624, 831, 987, 1293
SacI GAGCTC 1 cut(s) 240
SapI GCTCTTC 1 cut(s) 954
SaqAI TTAA 6 cut(s) 282, 317, 570, 1125, 1248, 1506
SatI GCNGC 3 cut(s) 204, 744, 1410
Sau3AI GATC 5 cut(s) 445, 531, 880, 1129, 1315
Sau96I GGNCC 1 cut(s) 1010
ScrFI CCNGG 4 cut(s) 897, 941, 1014, 1342
SduI GDGCHC 7 cut(s) 23, 240, 278, 420, 841, 928, 1153
SfaNI GCATC 8 cut(s) 134, 547, 657, 823, 1150, 1231, 1399, 1659
SfcI CTRYAG 3 cut(s) 79, 648, 1513
Sfr274I CTCGAG 1 cut(s) 1056
SfuI TTCGAA 1 cut(s) 1560
SlaI CTCGAG 1 cut(s) 1056
SmiMI CAYNNNNRTG 5 cut(s) 246, 624, 831, 987, 1293
SmlI CTYRAG 2 cut(s) 1056, 1403
SmoI CTYRAG 2 cut(s) 1056, 1403
SseBI AGGCCT 1 cut(s) 1426
SsiI CCGC 3 cut(s) 24, 743, 1531
SspI AATATT 3 cut(s) 220, 1354, 1436
SspMI CTAG 3 cut(s) 425, 965, 1488
SstI GAGCTC 1 cut(s) 240
StuI AGGCCT 1 cut(s) 1426
StyD4I CCNGG 4 cut(s) 895, 939, 1012, 1340
StyI CCWWGG 4 cut(s) 411, 1170, 1323, 1626
TaaI ACNGT 5 cut(s) 847, 948, 1021, 1552, 1662
TaiI ACGT 1 cut(s) 292
TaqI TCGA 4 cut(s) 522, 1057, 1546, 1560
TatI WGTACW 1 cut(s) 1603
TauI GCSGC 1 cut(s) 746
TfiI GAWTC 4 cut(s) 296, 433, 1373, 1399
Tru1I TTAA 6 cut(s) 282, 317, 570, 1125, 1248, 1506
Tru9I TTAA 6 cut(s) 282, 317, 570, 1125, 1248, 1506
TscAI CASTG 3 cut(s) 979, 1273, 1383
TseFI GTSAC 3 cut(s) 820, 1080, 1135
TseI GCWGC 2 cut(s) 203, 1409
Tsp45I GTSAC 3 cut(s) 820, 1080, 1135
TspDTI ATGAA 7 cut(s) 335, 698, 975, 1077, 1157, 1220, 1446
TspGWI ACGGA 1 cut(s) 732
TspRI CASTG 3 cut(s) 979, 1273, 1383
Van91I CCANNNNNTGG 1 cut(s) 260
VneI GTGCAC 3 cut(s) 274, 837, 924
VspI ATTAAT 1 cut(s) 317
XapI RAATTY 3 cut(s) 180, 792, 1045
XhoI CTCGAG 1 cut(s) 1056
XmiI GTMKAC 2 cut(s) 78, 351
XspI CTAG 3 cut(s) 425, 965, 1488
Zsp2I ATGCAT 1 cut(s) 1246
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.