MD05G1027100.v1.1

acyl-activating enzyme 1

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr05
OG0014332
Physical Location & Seq
Forward (+)
4300677 .. 4304302
3626 bp
Loading structure...
UTR
Exon/CDS
Intron
MD05G1027100.v1.1.491

Sequence Viewer

Length: 1665 bp
ATGGAGGGTTCGATCAGGTGCTCTGCCAACTACATCCCTCTTTCTCCGATCAGCTTCTTGGAGCGCTCCGCCATAGTCTACAGAGACAGGCCATCTGTTGTGTATGGAAACATCGTCTACACTTGGAGACAGACACTTGAACGATGCACCAGACTCGCTTCTGCTCTTGCCCAGCTCGGAGTTTCTTACGGAGATGTGGTTGCTGCATTGGCTCCAAATATTCCCGCAATGTATGAGCTCCATTTTGGTGTTCCAATGGCAGGGGCAGTTCTCTGTACACTTAATATACGCCATGATTCAGCAATGGTGTCAGTGTTACTAAAACATTCAGATGCCAAAATCATTTTTGTAGACTACCAATTGCTTGATGTTGCTAAAGGAGCAATTGATATTCTATCCAAGACTAGGACCAAGCTGCCCGTTTTAGTCTTAATCCCAGAGAGTGATCAATCATCCCCTGACTTCTGTAATCCCACTTCCGGGAACTTGGATTATGAGAGCCTGTTAGGACGTGGAAATTTTGATTTTGAGATCCGACGACCAAAAGATGAATGGGATGCAATTTCACTCAACTACACTTCAGGCACTACATCGAGCCCAAAAGGCGTCATTTATAGTCACAGAGGTGCCTATCTTAATTCTTTCGCAGCAGCTCTACTTAATGAGATGAGCTCATTGCCTGTATATTTATGGTGTGTTCCCATGTTTCATTGCAACGGGTGGTGCCTCACTTGGGCTGTGGCTGCTCAGGGTGGCACTAATATCTGCCAAAGGAATGTCACTGCAAAGGGCATTTTTAGCCATATTTCTGAGCACAAGGTAACCCACATGGGTGGTGCACCAACAATTTTAAACATGATTGTAAATGCTCCGGTACATGAGCGGAGGCCACTTCCGGGTAAGGTAATAGTCATGACTGGGGGTGCACCGCCACCAGCCCACGTACTCTTCAAGATGGAAGAGCTAGGGTTCAGTGTAACCCATTCATATGGTTTGACAGAAACTTATGGACCTGGTACAGTTTGTGCTTGGAAACCTGAATGGGACTCCCTGCCTCGAGATGAACAGGCAAAGATCAAGTCCCGGCAGGGATTGCAACATCTTGGCATGGAGGAGGTTGACGTTAAAGATCCCGTCACCATGAAAACTGTACCATCTGATGCAAAAACCATGGGGGAGGTTATGTTCAGAGGTAACACTGTAATGAATGGATATTTGAAAGATCATCAAGCAACGACAGATGCATTTAAAGGGGGATGGTTTCGTAGTGGTGACTTGGGTGTGAAACACCCGGATGGTTACGTAGAGCTAAAGGATCGTTTAAAGGACATTATCATTTCTGGGGGTGAAAATATTAGCACAATTGAGGTTGAATCAGTGCTCTTCAGCCACCCAGATATTCTCGAGGCAGCAATTGTGGGAAGGCCTGATGATTATTGGGGGGAGACGCCCTGCGCGTTTGTGAAGTTGAAGGATGGCTGTAATACTGACAAGGAGGAGATTATTAAGTTTTGTAGGAATCGGTTGCCCCACTATATGGCTCCTCGGACTGTTGTTTTCTCAGATCTGCCAAAGACTTCGACTGGGAAGGTGCAGAAGTATGTACTGAGAGAGAGGGCAAAGTCCATGGGAAGTCTCTCCAAGAACAGCATCAGCAAACTGTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005777 GO:0042579 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0044424 GO:0044444 GO:0044464
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
AMP-binding AMP-binding_C

Protein Analysis

555

Amino Acids

61.14

Weight (kDa)

7.58

Isoelectric Point (pI)

33.02

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
AMP-binding PF00501 20 - 406 4.1e-74 AMP-binding enzyme
AMP-binding_C PF13193 456 - 530 2.1e-22 AMP-binding enzyme C-terminal domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0014332)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G20560 AT1G20560
fragaria_vesca FvH4_2g13610 FvH4_2g13610 FvH4_2g13610 FvH4_2g13610
malus_domestica MD05G1027100.v1.1
prunus_persica Prupe.8G032600_v2.0.a1 Prupe.8G032600_v2.0.a1
pyrus_communis pycom05g01910
rosa_chinensis RchiOBHm_Chr6g0275221
rosa_laevigata RLG00000013486
rosa_roxburghii Rroxscaffold_7G00193540
rosa_rugosa Rorug06G0090300
rosa_samantha Rh6AG203600 Rh6CG209000
rosa_wichuraiana Rw6G017670

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 2 cut(s) 626, 723
AccB7I CCANNNNNTGG 1 cut(s) 1537
AccBSI CCGCTC 1 cut(s) 883
AccI GTMKAC 3 cut(s) 78, 117, 351
AccII CGCG 1 cut(s) 1457
AciI CCGC 4 cut(s) 69, 225, 883, 929
AclWI GGATC 3 cut(s) 526, 1124, 1323
AcsI RAATTY 1 cut(s) 517
AcuI CTGAAG 2 cut(s) 564, 1369
AcyI GRCGYC 2 cut(s) 606, 1448
AfaI GTAC 6 cut(s) 277, 876, 945, 1018, 1152, 1605
AfeI AGCGCT 1 cut(s) 65
AfiI CCNNNNNNNGG 7 cut(s) 260, 405, 479, 480, 733, 896, 1537
AgsI TTSAA 5 cut(s) 140, 952, 1219, 1373, 1471
AjiI CACGTC 1 cut(s) 512
AjnI CCWGG 1 cut(s) 1012
AleI CACNNNNGTG 2 cut(s) 624, 831
AloI GAACNNNNNNTCC 2 cut(s) 1169, 1201
AluBI AGCT 8 cut(s) 54, 175, 238, 415, 653, 672, 964, 1309
AluI AGCT 8 cut(s) 54, 175, 238, 415, 653, 672, 964, 1309
Alw21I GWGCWC 7 cut(s) 23, 240, 674, 816, 841, 928, 1383
Alw26I GTCTC 4 cut(s) 78, 121, 1439, 1640
Alw44I GTGCAC 2 cut(s) 837, 924
AlwI GGATC 3 cut(s) 526, 1124, 1323
Ama87I CYCGRG 2 cut(s) 1056, 1403
Aor51HI AGCGCT 1 cut(s) 65
AoxI GGCC 3 cut(s) 89, 887, 1424
ApaLI GTGCAC 2 cut(s) 837, 924
ApeKI GCWGC 6 cut(s) 203, 415, 647, 650, 743, 1409
ApoI RAATTY 1 cut(s) 517
ArsI GACNNNNNNTTYG 2 cut(s) 1064, 1096
AspLEI GCGC 2 cut(s) 66, 1457
AspS9I GGNCC 2 cut(s) 408, 1010
AsuC2I CCSGG 4 cut(s) 481, 897, 1084, 1292
AsuHPI GGTGA 3 cut(s) 1129, 1283, 1358
AvaI CYCGRG 2 cut(s) 1056, 1403
AvaII GGWCC 2 cut(s) 408, 1010
BaeGI GKGCMC 2 cut(s) 841, 928
BanI GGYRCC 2 cut(s) 626, 723
BanII GRGCYC 3 cut(s) 240, 599, 674
BarI GAAGNNNNNNTAC 2 cut(s) 460, 492
Bbv12I GWGCWC 7 cut(s) 23, 240, 674, 816, 841, 928, 1383
BbvI GCAGC 6 cut(s) 190, 402, 659, 662, 730, 1421
BccI CCATC 6 cut(s) 100, 949, 1162, 1251, 1289, 1469
BcgI CGANNNNNNTGC 2 cut(s) 136, 170
BciT130I CCWGG 1 cut(s) 1014
BclI TGATCA 1 cut(s) 445
BcnI CCSGG 4 cut(s) 481, 897, 1084, 1292
BcoDI GTCTC 4 cut(s) 78, 121, 1439, 1640
BfaI CTAG 2 cut(s) 405, 965
BfmI CTRYAG 1 cut(s) 79
BfoI RGCGCY 1 cut(s) 67
BglI GCCNNNNNGGC 1 cut(s) 603
BglII AGATCT 1 cut(s) 1564
BisI GCNGC 6 cut(s) 204, 416, 648, 651, 744, 1410
BlsI GCNGC 6 cut(s) 205, 417, 649, 652, 745, 1411
Bme1390I CCNGG 5 cut(s) 481, 897, 1014, 1084, 1292
Bme18I GGWCC 2 cut(s) 408, 1010
BmeT110I CYCGRG 2 cut(s) 1056, 1403
BmgBI CACGTC 1 cut(s) 512
BmgT120I GGNCC 2 cut(s) 408, 1010
BmiI GGNNCC 4 cut(s) 213, 628, 725, 1542
BmrFI CCNGG 5 cut(s) 481, 897, 1014, 1084, 1292
BmrI ACTGGG 2 cut(s) 927, 1593
BmsI GCATC 6 cut(s) 134, 322, 547, 1150, 1231, 1659
BmuI ACTGGG 2 cut(s) 927, 1593
BplI GAGNNNNNCTC 2 cut(s) 656, 688
Bpu10I CCTNAGC 1 cut(s) 747
BpuMI CCSGG 4 cut(s) 481, 897, 1084, 1292
BsaAI YACGTR 2 cut(s) 943, 1303
BsaHI GRCGYC 2 cut(s) 606, 1448
BsaJI CCNNGG 3 cut(s) 1170, 1544, 1626
BsaWI WCCGGW 1 cut(s) 871
BsaXI ACNNNNNCTCC 4 cut(s) 183, 213, 1169, 1199
Bsc4I CCNNNNNNNGG 7 cut(s) 260, 405, 479, 480, 733, 896, 1537
Bse1I ACTGG 2 cut(s) 922, 1588
Bse3DI GCAATG 4 cut(s) 234, 309, 674, 709
BseBI CCWGG 1 cut(s) 1014
BseDI CCNNGG 3 cut(s) 1170, 1544, 1626
BseGI GGATG 6 cut(s) 33, 452, 562, 1262, 1300, 1480
BseLI CCNNNNNNNGG 7 cut(s) 260, 405, 479, 480, 733, 896, 1537
BseMI GCAATG 4 cut(s) 234, 309, 674, 709
BseMII CTCAG 4 cut(s) 761, 801, 1575, 1598
BseNI ACTGG 2 cut(s) 922, 1588
BseRI GAGGAG 3 cut(s) 1127, 1511, 1533
BseSI GKGCMC 2 cut(s) 841, 928
BseXI GCAGC 6 cut(s) 190, 402, 659, 662, 730, 1421
BseYI CCCAGC 1 cut(s) 171
BsgI GTGCAG 1 cut(s) 1613
Bsh1236I CGCG 1 cut(s) 1457
BshFI GGCC 3 cut(s) 91, 889, 1426
BshNI GGYRCC 2 cut(s) 626, 723
BsiHKAI GWGCWC 7 cut(s) 23, 240, 674, 816, 841, 928, 1383
BsiHKCI CYCGRG 2 cut(s) 1056, 1403
BsiSI CCGG 5 cut(s) 480, 872, 896, 1084, 1292
BslFI GGGAC 2 cut(s) 1058, 1066
BslI CCNNNNNNNGG 7 cut(s) 260, 405, 479, 480, 733, 896, 1537
BsmAI GTCTC 4 cut(s) 78, 121, 1439, 1640
BsmBI CGTCTC 1 cut(s) 1439
BsmFI GGGAC 2 cut(s) 1058, 1066
BsnI GGCC 3 cut(s) 91, 889, 1426
BsoBI CYCGRG 2 cut(s) 1056, 1403
Bsp1286I GDGCHC 8 cut(s) 23, 240, 599, 674, 816, 841, 928, 1383
Bsp1407I TGTACA 1 cut(s) 275
Bsp143I GATC 9 cut(s) 12, 48, 445, 531, 1074, 1129, 1222, 1315, 1564
Bsp19I CCATGG 2 cut(s) 1170, 1626
BspACI CCGC 4 cut(s) 69, 225, 883, 929
BspANI GGCC 3 cut(s) 91, 889, 1426
BspCNI CTCAG 4 cut(s) 760, 802, 1574, 1599
BspFNI CGCG 1 cut(s) 1457
BspHI TCATGA 1 cut(s) 912
BspLI GGNNCC 4 cut(s) 213, 628, 725, 1542
BspPI GGATC 3 cut(s) 526, 1124, 1323
BspQI GCTCTTC 2 cut(s) 954, 1388
BspT107I GGYRCC 2 cut(s) 626, 723
BsrBI CCGCTC 1 cut(s) 883
BsrDI GCAATG 4 cut(s) 234, 309, 674, 709
BsrGI TGTACA 1 cut(s) 275
BsrI ACTGG 2 cut(s) 922, 1588
BssECI CCNNGG 3 cut(s) 1170, 1544, 1626
BssMI GATC 9 cut(s) 12, 48, 445, 531, 1074, 1129, 1222, 1315, 1564
BssNI GRCGYC 2 cut(s) 606, 1448
BssT1I CCWWGG 2 cut(s) 1170, 1626
Bst2UI CCWGG 1 cut(s) 1014
Bst4CI ACNGT 5 cut(s) 1021, 1150, 1201, 1552, 1662
Bst6I CTCTTC 3 cut(s) 953, 954, 1388
BstACI GRCGYC 2 cut(s) 606, 1448
BstAPI GCANNNNNTGC 1 cut(s) 1093
BstAUI TGTACA 1 cut(s) 275
BstBAI YACGTR 2 cut(s) 943, 1303
BstDEI CTNAG 4 cut(s) 747, 810, 1561, 1607
BstDSI CCRYGG 2 cut(s) 1170, 1626
BstEII GGTNACC 1 cut(s) 820
BstF5I GGATG 6 cut(s) 33, 452, 562, 1262, 1300, 1480
BstFNI CGCG 1 cut(s) 1457
BstH2I RGCGCY 1 cut(s) 67
BstHHI GCGC 2 cut(s) 66, 1457
BstKTI GATC 9 cut(s) 15, 51, 448, 534, 1077, 1132, 1225, 1318, 1567
BstMAI GTCTC 4 cut(s) 78, 121, 1439, 1640
BstMBI GATC 9 cut(s) 12, 48, 445, 531, 1074, 1129, 1222, 1315, 1564
BstMWI GCNNNNNNNGC 6 cut(s) 209, 380, 603, 743, 798, 1093
BstNI CCWGG 1 cut(s) 1014
BstPI GGTNACC 1 cut(s) 820
BstSCI CCNGG 5 cut(s) 479, 895, 1012, 1082, 1290
BstSFI CTRYAG 1 cut(s) 79
BstSLI GKGCMC 2 cut(s) 841, 928
BstSNI TACGTA 1 cut(s) 1303
BstUI CGCG 1 cut(s) 1457
BstV1I GCAGC 6 cut(s) 190, 402, 659, 662, 730, 1421
BstX2I RGATCY 3 cut(s) 531, 1129, 1564
BstXI CCANNNNNNTGG 2 cut(s) 833, 989
BstYI RGATCY 3 cut(s) 531, 1129, 1564
BsuRI GGCC 3 cut(s) 91, 889, 1426
BtgI CCRYGG 2 cut(s) 1170, 1626
BtrI CACGTC 1 cut(s) 512
BtsCI GGATG 6 cut(s) 33, 452, 562, 1262, 1300, 1480
BtsI GCAGTG 1 cut(s) 780
BtsIMutI CAGTG 5 cut(s) 318, 780, 979, 1197, 1383
CciI TCATGA 1 cut(s) 912
CfoI GCGC 2 cut(s) 66, 1457
Cfr13I GGNCC 2 cut(s) 408, 1010
CseI GACGC 2 cut(s) 595, 1456
CsiI ACCWGGT 1 cut(s) 1012
Csp6I GTAC 6 cut(s) 276, 875, 944, 1017, 1151, 1604
CviQI GTAC 6 cut(s) 276, 875, 944, 1017, 1151, 1604
DdeI CTNAG 4 cut(s) 747, 810, 1561, 1607
DpnI GATC 9 cut(s) 14, 50, 447, 533, 1076, 1131, 1224, 1317, 1566
DpnII GATC 9 cut(s) 12, 48, 445, 531, 1074, 1129, 1222, 1315, 1564
DraI TTTAAA 3 cut(s) 852, 1249, 1323
Eam1104I CTCTTC 3 cut(s) 953, 954, 1388
EarI CTCTTC 3 cut(s) 953, 954, 1388
EciI GGCGGA 1 cut(s) 58
Ecl136II GAGCTC 2 cut(s) 238, 672
Eco105I TACGTA 1 cut(s) 1303
Eco130I CCWWGG 2 cut(s) 1170, 1626
Eco147I AGGCCT 1 cut(s) 1426
Eco24I GRGCYC 3 cut(s) 240, 599, 674
Eco47I GGWCC 2 cut(s) 408, 1010
Eco47III AGCGCT 1 cut(s) 65
Eco53kI GAGCTC 2 cut(s) 238, 672
Eco57I CTGAAG 2 cut(s) 564, 1369
Eco88I CYCGRG 2 cut(s) 1056, 1403
Eco91I GGTNACC 1 cut(s) 820
EcoICRI GAGCTC 2 cut(s) 238, 672
EcoO65I GGTNACC 1 cut(s) 820
EcoRII CCWGG 1 cut(s) 1012
EcoT14I CCWWGG 2 cut(s) 1170, 1626
EcoT22I ATGCAT 1 cut(s) 1246
EcoT38I GRGCYC 3 cut(s) 240, 599, 674
ErhI CCWWGG 2 cut(s) 1170, 1626
Esp3I CGTCTC 1 cut(s) 1439
FaqI GGGAC 2 cut(s) 1058, 1066
FauI CCCGC 1 cut(s) 232
FauNDI CATATG 1 cut(s) 988
FbaI TGATCA 1 cut(s) 445
FblI GTMKAC 3 cut(s) 78, 117, 351
Fnu4HI GCNGC 6 cut(s) 204, 416, 648, 651, 744, 1410
FokI GGATG 6 cut(s) 20, 439, 569, 1269, 1307, 1487
FriOI GRGCYC 3 cut(s) 240, 599, 674
Fsp4HI GCNGC 6 cut(s) 204, 416, 648, 651, 744, 1410
FspBI CTAG 2 cut(s) 405, 965
GlaI GCGC 2 cut(s) 65, 1456
GluI GCNGC 6 cut(s) 204, 416, 648, 651, 744, 1410
GsaI CCCAGC 1 cut(s) 175
HaeII RGCGCY 1 cut(s) 67
HaeIII GGCC 3 cut(s) 91, 889, 1426
HapII CCGG 5 cut(s) 480, 872, 896, 1084, 1292
HgaI GACGC 2 cut(s) 595, 1456
HhaI GCGC 2 cut(s) 66, 1457
Hin1I GRCGYC 2 cut(s) 606, 1448
Hin6I GCGC 2 cut(s) 64, 1455
HinP1I GCGC 2 cut(s) 64, 1455
HincII GTYRAC 1 cut(s) 1120
HindII GTYRAC 1 cut(s) 1120
HinfI GANTC 5 cut(s) 153, 296, 1046, 1373, 1519
HpaII CCGG 5 cut(s) 480, 872, 896, 1084, 1292
HphI GGTGA 3 cut(s) 1129, 1283, 1358
Hpy166II GTNNAC 7 cut(s) 79, 118, 278, 352, 839, 926, 1120
Hpy188I TCNGA 9 cut(s) 48, 179, 331, 536, 811, 1159, 1190, 1548, 1564
Hpy188III TCNNGA 4 cut(s) 913, 952, 1058, 1403
Hpy8I GTNNAC 7 cut(s) 79, 118, 278, 352, 839, 926, 1120
Hpy99I CGWCG 1 cut(s) 540
HpyAV CCTTC 3 cut(s) 1416, 1465, 1582
HpyCH4III ACNGT 5 cut(s) 1021, 1150, 1201, 1552, 1662
HpyCH4IV ACGT 4 cut(s) 511, 942, 1122, 1302
HpyF10VI GCNNNNNNNGC 6 cut(s) 209, 380, 603, 743, 798, 1093
HpyF3I CTNAG 4 cut(s) 747, 810, 1561, 1607
HpySE526I ACGT 4 cut(s) 511, 942, 1122, 1302
Hsp92I GRCGYC 2 cut(s) 606, 1448
HspAI GCGC 2 cut(s) 64, 1455
Ksp22I TGATCA 1 cut(s) 445
Kzo9I GATC 9 cut(s) 12, 48, 445, 531, 1074, 1129, 1222, 1315, 1564
LguI GCTCTTC 2 cut(s) 954, 1388
LmnI GCTCC 7 cut(s) 61, 71, 217, 243, 380, 874, 1546
Lsp1109I GCAGC 6 cut(s) 190, 402, 659, 662, 730, 1421
LweI GCATC 6 cut(s) 134, 322, 547, 1150, 1231, 1659
MabI ACCWGGT 1 cut(s) 1012
MaeI CTAG 2 cut(s) 405, 965
MaeII ACGT 4 cut(s) 511, 942, 1122, 1302
MaeIII GTNAC 9 cut(s) 315, 617, 778, 820, 976, 1135, 1193, 1271, 1298
MalI GATC 9 cut(s) 14, 50, 447, 533, 1076, 1131, 1224, 1317, 1566
MbiI CCGCTC 1 cut(s) 883
MboI GATC 9 cut(s) 12, 48, 445, 531, 1074, 1129, 1222, 1315, 1564
MboII GAAGA 3 cut(s) 940, 971, 1375
MfeI CAATTG 4 cut(s) 359, 384, 1362, 1413
MflI RGATCY 3 cut(s) 531, 1129, 1564
MhlI GDGCHC 8 cut(s) 23, 240, 599, 674, 816, 841, 928, 1383
MluCI AATT 8 cut(s) 359, 384, 517, 561, 637, 846, 1362, 1413
MlyI GAGTC 2 cut(s) 147, 1040
MmeI TCCRAC 1 cut(s) 559
Mph1103I ATGCAT 1 cut(s) 1246
MseI TTAA 9 cut(s) 282, 431, 636, 660, 851, 1125, 1248, 1322, 1506
MslI CAYNNNNRTG 7 cut(s) 246, 330, 624, 831, 987, 1202, 1293
MspI CCGG 5 cut(s) 480, 872, 896, 1084, 1292
MspR9I CCNGG 5 cut(s) 481, 897, 1014, 1084, 1292
MunI CAATTG 4 cut(s) 359, 384, 1362, 1413
MvaI CCWGG 1 cut(s) 1014
MvnI CGCG 1 cut(s) 1457
MwoI GCNNNNNNNGC 6 cut(s) 209, 380, 603, 743, 798, 1093
NciI CCSGG 4 cut(s) 481, 897, 1084, 1292
NcoI CCATGG 2 cut(s) 1170, 1626
NdeI CATATG 1 cut(s) 988
NdeII GATC 9 cut(s) 12, 48, 445, 531, 1074, 1129, 1222, 1315, 1564
NlaIV GGNNCC 4 cut(s) 213, 628, 725, 1542
NmuCI GTSAC 4 cut(s) 617, 778, 1135, 1271
NsiI ATGCAT 1 cut(s) 1246
OliI CACNNNNGTG 2 cut(s) 624, 831
PaeR7I CTCGAG 2 cut(s) 1056, 1403
PagI TCATGA 1 cut(s) 912
PceI AGGCCT 1 cut(s) 1426
PciSI GCTCTTC 2 cut(s) 954, 1388
PcsI WCGNNNNNNNCGW 1 cut(s) 1454
PfeI GAWTC 3 cut(s) 296, 1373, 1519
PflMI CCANNNNNTGG 1 cut(s) 1537
PfoI TCCNGGA 1 cut(s) 479
PkrI GCNGC 6 cut(s) 205, 417, 649, 652, 745, 1411
PleI GAGTC 2 cut(s) 147, 1040
PpsI GAGTC 2 cut(s) 147, 1040
Ppu21I YACGTR 2 cut(s) 943, 1303
Psp124BI GAGCTC 2 cut(s) 240, 674
Psp6I CCWGG 1 cut(s) 1012
PspEI GGTNACC 1 cut(s) 820
PspFI CCCAGC 1 cut(s) 171
PspGI CCWGG 1 cut(s) 1012
PspN4I GGNNCC 4 cut(s) 213, 628, 725, 1542
PspPI GGNCC 2 cut(s) 408, 1010
PsuI RGATCY 3 cut(s) 531, 1129, 1564
RsaI GTAC 6 cut(s) 277, 876, 945, 1018, 1152, 1605
RsaNI GTAC 6 cut(s) 276, 875, 944, 1017, 1151, 1604
RseI CAYNNNNRTG 7 cut(s) 246, 330, 624, 831, 987, 1202, 1293
SacI GAGCTC 2 cut(s) 240, 674
SapI GCTCTTC 2 cut(s) 954, 1388
SaqAI TTAA 9 cut(s) 282, 431, 636, 660, 851, 1125, 1248, 1322, 1506
SatI GCNGC 6 cut(s) 204, 416, 648, 651, 744, 1410
Sau3AI GATC 9 cut(s) 12, 48, 445, 531, 1074, 1129, 1222, 1315, 1564
Sau96I GGNCC 2 cut(s) 408, 1010
SchI GAGTC 2 cut(s) 147, 1040
ScrFI CCNGG 5 cut(s) 481, 897, 1014, 1084, 1292
SduI GDGCHC 8 cut(s) 23, 240, 599, 674, 816, 841, 928, 1383
SexAI ACCWGGT 1 cut(s) 1012
SfaNI GCATC 6 cut(s) 134, 322, 547, 1150, 1231, 1659
SfcI CTRYAG 1 cut(s) 79
Sfr274I CTCGAG 2 cut(s) 1056, 1403
SinI GGWCC 2 cut(s) 408, 1010
SlaI CTCGAG 2 cut(s) 1056, 1403
SmiMI CAYNNNNRTG 7 cut(s) 246, 330, 624, 831, 987, 1202, 1293
SmlI CTYRAG 2 cut(s) 1056, 1403
SmoI CTYRAG 2 cut(s) 1056, 1403
SnaBI TACGTA 1 cut(s) 1303
Sse9I AATT 8 cut(s) 359, 384, 517, 561, 637, 846, 1362, 1413
SseBI AGGCCT 1 cut(s) 1426
SsiI CCGC 4 cut(s) 69, 225, 883, 929
SspI AATATT 2 cut(s) 220, 1354
SspMI CTAG 2 cut(s) 405, 965
SstI GAGCTC 2 cut(s) 240, 674
StuI AGGCCT 1 cut(s) 1426
StyD4I CCNGG 5 cut(s) 479, 895, 1012, 1082, 1290
StyI CCWWGG 2 cut(s) 1170, 1626
TaaI ACNGT 5 cut(s) 1021, 1150, 1201, 1552, 1662
TaiI ACGT 4 cut(s) 514, 945, 1125, 1305
TaqI TCGA 5 cut(s) 11, 593, 1057, 1404, 1580
TasI AATT 8 cut(s) 359, 384, 517, 561, 637, 846, 1362, 1413
TatI WGTACW 2 cut(s) 275, 1603
TfiI GAWTC 3 cut(s) 296, 1373, 1519
Tru1I TTAA 9 cut(s) 282, 431, 636, 660, 851, 1125, 1248, 1322, 1506
Tru9I TTAA 9 cut(s) 282, 431, 636, 660, 851, 1125, 1248, 1322, 1506
TscAI CASTG 5 cut(s) 318, 787, 979, 1204, 1383
TseFI GTSAC 4 cut(s) 617, 778, 1135, 1271
TseI GCWGC 6 cut(s) 203, 415, 647, 650, 743, 1409
Tsp45I GTSAC 4 cut(s) 617, 778, 1135, 1271
TspDTI ATGAA 6 cut(s) 564, 698, 975, 1077, 1157, 1220
TspGWI ACGGA 1 cut(s) 204
TspRI CASTG 5 cut(s) 318, 787, 979, 1204, 1383
Van91I CCANNNNNTGG 1 cut(s) 1537
VneI GTGCAC 2 cut(s) 837, 924
VpaK11BI GGWCC 2 cut(s) 408, 1010
XapI RAATTY 1 cut(s) 517
XcmI CCANNNNNNNNNTGG 1 cut(s) 549
XhoI CTCGAG 2 cut(s) 1056, 1403
XmiI GTMKAC 3 cut(s) 78, 117, 351
XspI CTAG 2 cut(s) 405, 965
Zsp2I ATGCAT 1 cut(s) 1246
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.