Rh6AG203600

acyl-activating enzyme 1

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr6A
OG0014332
Physical Location & Seq
Reverse (-)
36389266 .. 36393408
4143 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh6AG203600.1

Sequence Viewer

Length: 1437 bp
ATGTATGAGCTCCATTTTGGTGTTCCAATGGCTGGGGCAGTTTTGTGTACACTTAACGTACGTCATGATTCAAAAATGGTTTCAGTATTAATGAAGCATTCAGAAGCCAAAATCATTTTTGTAGACTACCAATTTATCCATGTTGCGAAGGGAGCATTTGATATCTTATCCAAGACTGGAACCAAGGTCCCCCTTCTAGTTTTAATTCCAGAGAGTGATCAATCATCTCCTGACTTCTGCAATCCCCCTTCTGCATACATAGAATATGAGAGACTCTTAGGAAAGGGAAATCTTGGTTTTGAGATCCTACGACCGAAAGACGAATGGGATGCGATTTCGCTCAACTACACTTCAGGCACTACTTCTAGCCCAAAAGGTGTCATTTATAGCCATAGAGGTGCGTATCTCAATTCTCTGGCAGGAGTTATCCTCAATGAGATGGTATCAATGCCTGTATATTTGTGGTGTGTTCCCATGTTTCATTGCAATGGATGGTGCCTCACTTGGGCTGTAGCTGCTCAAGGTGGCACTAATGTCTGCCAAAGAAATGTGACTGCAAAAGGAATTTTCACCAGTATATCTCAGCATCGGGTGACCCACATGGGTGGTGCACCTACTGTCTTAAACATGATTGTAAATGCACCTGAAAATGATCGGAGGCCACTTCCGGGCAAGGTAGTGGTCATGACCGGGGCTGCACCACCACCATCCCAGGTACTGTTCAAAATGGAAGAGCTAGGGTTCAGTGTAACACATGCATATGGTTTGACAGAGACTTATGGTCCTGGGACAGTTTGCGCTTGGAAACCTGAATGGGATTCCCTACCTCGAGATGAACAAGCAAAAATCAAGTCACGACAAGGGTTGCAACATATTGGATTGGAGGAACTTGATGTTAAAGATCCGGTCACGATGAAGAGTGTTCCATCTGATGCAAAAACCATGGGTGAGGTTATGTTCAGGGGCAACACTGTTATGAATGGATATTTGAAAGATAATAAATCAACACAGGATGCATTTGAAGGTGGATGGTTTCGCAGTGGGGACTTGGCGGTCAGACACCCAGATGGTTACATAGAGCTAAAGGATCGTTCCAAGGATATTATAATTTCCGGTGGAGAAAACATTAGCACAATTGAGGTGGAATCAGTGCTTTTCAGGCATCCAGATGTTCTTGAGGCAGCTATTGTGGGAAGGCCTGATGAATATTGGGGGGAGACACCCTGTGCTTTTGTGAAATTGAAGGATGGTTGTAATGCTAGTGCAGAGGATATCATTAAGTTCTGTAGGGATCGGTTACCCCACTACATGGCTCCTCGAACTGTTATATTCGAAGATTTGCCAAAGACTTCAACTGGGAAGGTGCAGAAGTATGTACTGAGAGAGAAAGCTAAGGCCATGGGAAGTTCCTCCAAGACCAGCATCAGCAAACTGTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005777 GO:0042579 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0044424 GO:0044444 GO:0044464
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
AMP-binding AMP-binding_C

Protein Analysis

478

Amino Acids

52.77

Weight (kDa)

6.89

Isoelectric Point (pI)

36.64

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
AMP-binding PF00501 1 - 330 5.6e-55 AMP-binding enzyme
AMP-binding_C PF13193 380 - 454 1.3e-22 AMP-binding enzyme C-terminal domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0014332)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G20560 AT1G20560
fragaria_vesca FvH4_2g13610 FvH4_2g13610 FvH4_2g13610 FvH4_2g13610
malus_domestica MD05G1027100.v1.1
prunus_persica Prupe.8G032600_v2.0.a1 Prupe.8G032600_v2.0.a1
pyrus_communis pycom05g01910
rosa_chinensis RchiOBHm_Chr6g0275221
rosa_laevigata RLG00000013486
rosa_roxburghii Rroxscaffold_7G00193540
rosa_rugosa Rorug06G0090300
rosa_samantha Rh6AG203600 Rh6CG209000
rosa_wichuraiana Rw6G017670

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 1106
AasI GACNNNNNNGTC 1 cut(s) 1052
AccB1I GGYRCC 1 cut(s) 495
AccB7I CCANNNNNTGG 2 cut(s) 32, 1309
AccI GTMKAC 1 cut(s) 123
AciI CCGC 1 cut(s) 1052
AclWI GGATC 4 cut(s) 298, 896, 1095, 1299
AcsI RAATTY 1 cut(s) 564
AcuI CTGAAG 1 cut(s) 336
AdeI CACNNNGTG 1 cut(s) 1226
AfaI GTAC 4 cut(s) 49, 60, 717, 1377
AfiI CCNNNNNNNGG 4 cut(s) 32, 505, 668, 1309
AgsI TTSAA 6 cut(s) 72, 724, 991, 1022, 1243, 1353
AhdI GACNNNNNGTC 1 cut(s) 780
AjnI CCWGG 2 cut(s) 711, 784
AleI CACNNNNGTG 1 cut(s) 603
AluBI AGCT 6 cut(s) 10, 515, 736, 1081, 1184, 1391
AluI AGCT 6 cut(s) 10, 515, 736, 1081, 1184, 1391
Alw21I GWGCWC 2 cut(s) 12, 613
Alw26I GTCTC 3 cut(s) 265, 767, 1211
Alw44I GTGCAC 1 cut(s) 609
AlwI GGATC 4 cut(s) 298, 896, 1095, 1299
AlwNI CAGNNNCTG 1 cut(s) 718
Ama87I CYCGRG 1 cut(s) 828
AoxI GGCC 3 cut(s) 659, 1196, 1395
ApaLI GTGCAC 1 cut(s) 609
ApeKI GCWGC 3 cut(s) 515, 695, 1181
ApoI RAATTY 1 cut(s) 564
ArsI GACNNNNNNTTYG 2 cut(s) 836, 868
AseI ATTAAT 1 cut(s) 89
AspLEI GCGC 1 cut(s) 800
AspS9I GGNCC 2 cut(s) 187, 782
AsuC2I CCSGG 2 cut(s) 669, 691
AsuHPI GGTGA 3 cut(s) 562, 604, 959
AsuII TTCGAA 1 cut(s) 1332
AvaI CYCGRG 1 cut(s) 828
AvaII GGWCC 2 cut(s) 187, 782
BaeGI GKGCMC 1 cut(s) 613
BanI GGYRCC 1 cut(s) 495
BanII GRGCYC 1 cut(s) 12
Bbv12I GWGCWC 2 cut(s) 12, 613
BbvI GCAGC 3 cut(s) 502, 682, 1193
BccI CCATC 7 cut(s) 433, 486, 715, 934, 1023, 1061, 1241
BcgI CGANNNNNNTGC 2 cut(s) 311, 345
BciT130I CCWGG 2 cut(s) 713, 786
BclI TGATCA 1 cut(s) 217
BcnI CCSGG 2 cut(s) 669, 691
BcoDI GTCTC 3 cut(s) 265, 767, 1211
BfaI CTAG 4 cut(s) 197, 366, 737, 1260
BfmI CTRYAG 2 cut(s) 510, 1285
BisI GCNGC 3 cut(s) 516, 696, 1182
BlsI GCNGC 3 cut(s) 517, 697, 1183
Bme1390I CCNGG 4 cut(s) 669, 691, 713, 786
Bme18I GGWCC 2 cut(s) 187, 782
BmeRI GACNNNNNGTC 1 cut(s) 780
BmeT110I CYCGRG 1 cut(s) 828
BmgT120I GGNCC 2 cut(s) 187, 782
BmiI GGNNCC 4 cut(s) 181, 189, 497, 1314
BmrFI CCNGG 4 cut(s) 669, 691, 713, 786
BmrI ACTGGG 1 cut(s) 1365
BmsI GCATC 6 cut(s) 319, 595, 922, 1003, 1171, 1431
BmuI ACTGGG 1 cut(s) 1365
BplI GAGNNNNNCTC 2 cut(s) 414, 446
Bpu10I CCTNAGC 1 cut(s) 1392
Bpu14I TTCGAA 1 cut(s) 1332
BpuEI CTTGAG 2 cut(s) 504, 1196
BpuMI CCSGG 2 cut(s) 669, 691
BsaJI CCNNGG 7 cut(s) 183, 690, 711, 785, 942, 1095, 1398
BsaWI WCCGGW 2 cut(s) 904, 1112
Bsc4I CCNNNNNNNGG 4 cut(s) 32, 505, 668, 1309
Bse1I ACTGG 3 cut(s) 181, 573, 1360
Bse3DI GCAATG 2 cut(s) 481, 493
BseBI CCWGG 2 cut(s) 713, 786
BseDI CCNNGG 7 cut(s) 183, 690, 711, 785, 942, 1095, 1398
BseGI GGATG 7 cut(s) 334, 497, 707, 1018, 1034, 1162, 1252
BseLI CCNNNNNNNGG 4 cut(s) 32, 505, 668, 1309
BseMI GCAATG 2 cut(s) 481, 493
BseMII CTCAG 2 cut(s) 596, 1370
BseNI ACTGG 3 cut(s) 181, 573, 1360
BseRI GAGGAG 1 cut(s) 1305
BseSI GKGCMC 1 cut(s) 613
BseXI GCAGC 3 cut(s) 502, 682, 1193
BseYI CCCAGC 1 cut(s) 32
BsgI GTGCAG 3 cut(s) 681, 1284, 1385
Bsh1285I CGRYCG 1 cut(s) 314
BshFI GGCC 3 cut(s) 661, 1198, 1397
BshNI GGYRCC 1 cut(s) 495
BsiEI CGRYCG 1 cut(s) 314
BsiHKAI GWGCWC 2 cut(s) 12, 613
BsiHKCI CYCGRG 1 cut(s) 828
BsiSI CCGG 4 cut(s) 668, 690, 905, 1113
BsiWI CGTACG 1 cut(s) 58
BslFI GGGAC 3 cut(s) 173, 802, 1058
BslI CCNNNNNNNGG 4 cut(s) 32, 505, 668, 1309
BsmAI GTCTC 3 cut(s) 265, 767, 1211
BsmFI GGGAC 3 cut(s) 173, 802, 1058
BsmI GAATGC 1 cut(s) 97
BsnI GGCC 3 cut(s) 661, 1198, 1397
BsoBI CYCGRG 1 cut(s) 828
Bsp119I TTCGAA 1 cut(s) 1332
Bsp1286I GDGCHC 2 cut(s) 12, 613
Bsp1407I TGTACA 1 cut(s) 47
Bsp143I GATC 6 cut(s) 217, 303, 652, 901, 1087, 1291
Bsp19I CCATGG 2 cut(s) 942, 1398
BspACI CCGC 1 cut(s) 1052
BspANI GGCC 3 cut(s) 661, 1198, 1397
BspCNI CTCAG 2 cut(s) 595, 1371
BspHI TCATGA 2 cut(s) 64, 684
BspLI GGNNCC 4 cut(s) 181, 189, 497, 1314
BspPI GGATC 4 cut(s) 298, 896, 1095, 1299
BspQI GCTCTTC 1 cut(s) 726
BspT104I TTCGAA 1 cut(s) 1332
BspT107I GGYRCC 1 cut(s) 495
BsrDI GCAATG 2 cut(s) 481, 493
BsrGI TGTACA 1 cut(s) 47
BsrI ACTGG 3 cut(s) 181, 573, 1360
BssECI CCNNGG 7 cut(s) 183, 690, 711, 785, 942, 1095, 1398
BssMI GATC 6 cut(s) 217, 303, 652, 901, 1087, 1291
BssT1I CCWWGG 4 cut(s) 183, 942, 1095, 1398
Bst2UI CCWGG 2 cut(s) 713, 786
Bst4CI ACNGT 6 cut(s) 619, 720, 793, 973, 1324, 1434
Bst6I CTCTTC 2 cut(s) 726, 911
BstAUI TGTACA 1 cut(s) 47
BstBI TTCGAA 1 cut(s) 1332
BstDEI CTNAG 4 cut(s) 277, 582, 1379, 1392
BstDSI CCRYGG 2 cut(s) 942, 1398
BstEII GGTNACC 2 cut(s) 592, 1296
BstF5I GGATG 7 cut(s) 334, 497, 707, 1018, 1034, 1162, 1252
BstHHI GCGC 1 cut(s) 800
BstKTI GATC 6 cut(s) 220, 306, 655, 904, 1090, 1294
BstMAI GTCTC 3 cut(s) 265, 767, 1211
BstMBI GATC 6 cut(s) 217, 303, 652, 901, 1087, 1291
BstMCI CGRYCG 1 cut(s) 314
BstMWI GCNNNNNNNGC 3 cut(s) 152, 515, 1159
BstNI CCWGG 2 cut(s) 713, 786
BstNSI RCATGY 1 cut(s) 758
BstPI GGTNACC 2 cut(s) 592, 1296
BstSCI CCNGG 4 cut(s) 667, 689, 711, 784
BstSFI CTRYAG 2 cut(s) 510, 1285
BstSLI GKGCMC 1 cut(s) 613
BstV1I GCAGC 3 cut(s) 502, 682, 1193
BstX2I RGATCY 2 cut(s) 303, 901
BstXI CCANNNNNNTGG 1 cut(s) 605
BstYI RGATCY 2 cut(s) 303, 901
BsuRI GGCC 3 cut(s) 661, 1198, 1397
BtgI CCRYGG 2 cut(s) 942, 1398
BtsCI GGATG 7 cut(s) 334, 497, 707, 1018, 1034, 1162, 1252
BtsI GCAGTG 1 cut(s) 1045
BtsIMutI CAGTG 4 cut(s) 751, 969, 1045, 1155
CaiI CAGNNNCTG 1 cut(s) 718
CciI TCATGA 2 cut(s) 64, 684
CfoI GCGC 1 cut(s) 800
Cfr13I GGNCC 2 cut(s) 187, 782
Csp6I GTAC 4 cut(s) 48, 59, 716, 1376
CspCI CAANNNNNGTGG 2 cut(s) 1122, 1157
CviQI GTAC 4 cut(s) 48, 59, 716, 1376
DdeI CTNAG 4 cut(s) 277, 582, 1379, 1392
DpnI GATC 6 cut(s) 219, 305, 654, 903, 1089, 1293
DpnII GATC 6 cut(s) 217, 303, 652, 901, 1087, 1291
DraIII CACNNNGTG 1 cut(s) 1226
DrdI GACNNNNNNGTC 1 cut(s) 1052
DriI GACNNNNNGTC 1 cut(s) 780
DseDI GACNNNNNNGTC 1 cut(s) 1052
Eam1104I CTCTTC 2 cut(s) 726, 911
Eam1105I GACNNNNNGTC 1 cut(s) 780
EarI CTCTTC 2 cut(s) 726, 911
Ecl136II GAGCTC 1 cut(s) 10
Eco130I CCWWGG 4 cut(s) 183, 942, 1095, 1398
Eco147I AGGCCT 1 cut(s) 1198
Eco24I GRGCYC 1 cut(s) 12
Eco32I GATATC 2 cut(s) 163, 1273
Eco47I GGWCC 2 cut(s) 187, 782
Eco53kI GAGCTC 1 cut(s) 10
Eco57I CTGAAG 1 cut(s) 336
Eco88I CYCGRG 1 cut(s) 828
Eco91I GGTNACC 2 cut(s) 592, 1296
EcoICRI GAGCTC 1 cut(s) 10
EcoO109I RGGNCCY 1 cut(s) 187
EcoO65I GGTNACC 2 cut(s) 592, 1296
EcoRII CCWGG 2 cut(s) 711, 784
EcoRV GATATC 2 cut(s) 163, 1273
EcoT14I CCWWGG 4 cut(s) 183, 942, 1095, 1398
EcoT22I ATGCAT 2 cut(s) 760, 1018
EcoT38I GRGCYC 1 cut(s) 12
ErhI CCWWGG 4 cut(s) 183, 942, 1095, 1398
FaqI GGGAC 3 cut(s) 173, 802, 1058
FauNDI CATATG 1 cut(s) 760
FbaI TGATCA 1 cut(s) 217
FblI GTMKAC 1 cut(s) 123
Fnu4HI GCNGC 3 cut(s) 516, 696, 1182
FokI GGATG 7 cut(s) 341, 504, 694, 1025, 1041, 1149, 1259
FriOI GRGCYC 1 cut(s) 12
Fsp4HI GCNGC 3 cut(s) 516, 696, 1182
FspBI CTAG 4 cut(s) 197, 366, 737, 1260
GlaI GCGC 1 cut(s) 799
GluI GCNGC 3 cut(s) 516, 696, 1182
GsaI CCCAGC 1 cut(s) 36
HaeIII GGCC 3 cut(s) 661, 1198, 1397
HapII CCGG 4 cut(s) 668, 690, 905, 1113
HhaI GCGC 1 cut(s) 800
Hin6I GCGC 1 cut(s) 798
HinP1I GCGC 1 cut(s) 798
HinfI GANTC 4 cut(s) 68, 273, 818, 1145
HpaII CCGG 4 cut(s) 668, 690, 905, 1113
HphI GGTGA 3 cut(s) 562, 604, 959
Hpy166II GTNNAC 4 cut(s) 48, 50, 124, 611
Hpy188I TCNGA 4 cut(s) 103, 657, 931, 1058
Hpy188III TCNNGA 9 cut(s) 65, 209, 230, 685, 830, 855, 910, 1166, 1175
Hpy8I GTNNAC 4 cut(s) 48, 50, 124, 611
HpyAV CCTTC 7 cut(s) 142, 203, 258, 1016, 1188, 1237, 1354
HpyCH4III ACNGT 6 cut(s) 619, 720, 793, 973, 1324, 1434
HpyCH4IV ACGT 2 cut(s) 57, 61
HpyF10VI GCNNNNNNNGC 3 cut(s) 152, 515, 1159
HpyF3I CTNAG 4 cut(s) 277, 582, 1379, 1392
HpySE526I ACGT 2 cut(s) 57, 61
HspAI GCGC 1 cut(s) 798
Ksp22I TGATCA 1 cut(s) 217
Kzo9I GATC 6 cut(s) 217, 303, 652, 901, 1087, 1291
LguI GCTCTTC 1 cut(s) 726
LmnI GCTCC 3 cut(s) 15, 152, 1318
Lsp1109I GCAGC 3 cut(s) 502, 682, 1193
LweI GCATC 6 cut(s) 319, 595, 922, 1003, 1171, 1431
MaeI CTAG 4 cut(s) 197, 366, 737, 1260
MaeII ACGT 2 cut(s) 57, 61
MaeIII GTNAC 7 cut(s) 550, 592, 748, 852, 907, 1070, 1296
MalI GATC 6 cut(s) 219, 305, 654, 903, 1089, 1293
MboI GATC 6 cut(s) 217, 303, 652, 901, 1087, 1291
MboII GAAGA 3 cut(s) 743, 928, 1346
MfeI CAATTG 1 cut(s) 1134
MflI RGATCY 2 cut(s) 303, 901
MhlI GDGCHC 2 cut(s) 12, 613
MluCI AATT 7 cut(s) 131, 204, 409, 564, 1107, 1134, 1238
MlyI GAGTC 1 cut(s) 267
Mph1103I ATGCAT 2 cut(s) 760, 1018
MseI TTAA 6 cut(s) 54, 89, 203, 623, 897, 1278
MslI CAYNNNNRTG 8 cut(s) 18, 396, 486, 603, 759, 974, 1065, 1167
MspI CCGG 4 cut(s) 668, 690, 905, 1113
MspR9I CCNGG 4 cut(s) 669, 691, 713, 786
MunI CAATTG 1 cut(s) 1134
Mva1269I GAATGC 1 cut(s) 97
MvaI CCWGG 2 cut(s) 713, 786
MwoI GCNNNNNNNGC 3 cut(s) 152, 515, 1159
NciI CCSGG 2 cut(s) 669, 691
NcoI CCATGG 2 cut(s) 942, 1398
NdeI CATATG 1 cut(s) 760
NdeII GATC 6 cut(s) 217, 303, 652, 901, 1087, 1291
NlaIV GGNNCC 4 cut(s) 181, 189, 497, 1314
NmuCI GTSAC 4 cut(s) 550, 592, 852, 907
NsiI ATGCAT 2 cut(s) 760, 1018
NspI RCATGY 1 cut(s) 758
NspV TTCGAA 1 cut(s) 1332
OliI CACNNNNGTG 1 cut(s) 603
PaeR7I CTCGAG 1 cut(s) 828
PagI TCATGA 2 cut(s) 64, 684
PceI AGGCCT 1 cut(s) 1198
PciSI GCTCTTC 1 cut(s) 726
PctI GAATGC 1 cut(s) 97
PfeI GAWTC 3 cut(s) 68, 818, 1145
Pfl23II CGTACG 1 cut(s) 58
PflMI CCANNNNNTGG 2 cut(s) 32, 1309
PkrI GCNGC 3 cut(s) 517, 697, 1183
PleI GAGTC 1 cut(s) 267
PpsI GAGTC 1 cut(s) 267
PpuMI RGGWCCY 1 cut(s) 187
PshBI ATTAAT 1 cut(s) 89
PsiI TTATAA 1 cut(s) 1106
Psp124BI GAGCTC 1 cut(s) 12
Psp5II RGGWCCY 1 cut(s) 187
Psp6I CCWGG 2 cut(s) 711, 784
PspEI GGTNACC 2 cut(s) 592, 1296
PspFI CCCAGC 1 cut(s) 32
PspGI CCWGG 2 cut(s) 711, 784
PspLI CGTACG 1 cut(s) 58
PspN4I GGNNCC 4 cut(s) 181, 189, 497, 1314
PspPI GGNCC 2 cut(s) 187, 782
PspPPI RGGWCCY 1 cut(s) 187
PstNI CAGNNNCTG 1 cut(s) 718
PsuI RGATCY 2 cut(s) 303, 901
RsaI GTAC 4 cut(s) 49, 60, 717, 1377
RsaNI GTAC 4 cut(s) 48, 59, 716, 1376
RseI CAYNNNNRTG 8 cut(s) 18, 396, 486, 603, 759, 974, 1065, 1167
SacI GAGCTC 1 cut(s) 12
SapI GCTCTTC 1 cut(s) 726
SaqAI TTAA 6 cut(s) 54, 89, 203, 623, 897, 1278
SatI GCNGC 3 cut(s) 516, 696, 1182
Sau3AI GATC 6 cut(s) 217, 303, 652, 901, 1087, 1291
Sau96I GGNCC 2 cut(s) 187, 782
SchI GAGTC 1 cut(s) 267
ScrFI CCNGG 4 cut(s) 669, 691, 713, 786
SduI GDGCHC 2 cut(s) 12, 613
SfaNI GCATC 6 cut(s) 319, 595, 922, 1003, 1171, 1431
SfcI CTRYAG 2 cut(s) 510, 1285
Sfr274I CTCGAG 1 cut(s) 828
SfuI TTCGAA 1 cut(s) 1332
SinI GGWCC 2 cut(s) 187, 782
SlaI CTCGAG 1 cut(s) 828
SmiMI CAYNNNNRTG 8 cut(s) 18, 396, 486, 603, 759, 974, 1065, 1167
SmlI CTYRAG 3 cut(s) 519, 828, 1175
SmoI CTYRAG 3 cut(s) 519, 828, 1175
Sse9I AATT 7 cut(s) 131, 204, 409, 564, 1107, 1134, 1238
SseBI AGGCCT 1 cut(s) 1198
SsiI CCGC 1 cut(s) 1052
SspI AATATT 1 cut(s) 1208
SspMI CTAG 4 cut(s) 197, 366, 737, 1260
SstI GAGCTC 1 cut(s) 12
StuI AGGCCT 1 cut(s) 1198
StyD4I CCNGG 4 cut(s) 667, 689, 711, 784
StyI CCWWGG 4 cut(s) 183, 942, 1095, 1398
TaaI ACNGT 6 cut(s) 619, 720, 793, 973, 1324, 1434
TaiI ACGT 2 cut(s) 60, 64
TaqI TCGA 3 cut(s) 829, 1318, 1332
TaqII GACCGA 1 cut(s) 328
TasI AATT 7 cut(s) 131, 204, 409, 564, 1107, 1134, 1238
TatI WGTACW 2 cut(s) 47, 1375
TfiI GAWTC 3 cut(s) 68, 818, 1145
Tru1I TTAA 6 cut(s) 54, 89, 203, 623, 897, 1278
Tru9I TTAA 6 cut(s) 54, 89, 203, 623, 897, 1278
TscAI CASTG 4 cut(s) 751, 976, 1045, 1155
TseFI GTSAC 4 cut(s) 550, 592, 852, 907
TseI GCWGC 3 cut(s) 515, 695, 1181
Tsp45I GTSAC 4 cut(s) 550, 592, 852, 907
TspDTI ATGAA 6 cut(s) 107, 470, 849, 929, 992, 1218
TspRI CASTG 4 cut(s) 751, 976, 1045, 1155
Van91I CCANNNNNTGG 2 cut(s) 32, 1309
VneI GTGCAC 1 cut(s) 609
VpaK11BI GGWCC 2 cut(s) 187, 782
VspI ATTAAT 1 cut(s) 89
XapI RAATTY 1 cut(s) 564
XceI RCATGY 1 cut(s) 758
XhoI CTCGAG 1 cut(s) 828
XmiI GTMKAC 1 cut(s) 123
XspI CTAG 4 cut(s) 197, 366, 737, 1260
Zsp2I ATGCAT 2 cut(s) 760, 1018
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.