Prupe.8G032600_v2.0.a1

acyl-activating enzyme 1

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
prunus_persica
Pp08
OG0014332
Physical Location & Seq
Forward (+)
3059174 .. 3062736
3563 bp
Loading structure...
UTR
Exon/CDS
Intron
Prupe.8G032600.1

Sequence Viewer

Length: 1665 bp
ATGGAGGGTTCGATCAGGTGTTCCGCTAACTACATCCCCCTCTCTCCAATCAGCTTCCTGGAGCGCTCGGCCATAGTCTACAGAGACAGGCCTTCTGTTGTATACGGAAGCATCATCTACACTTGGAGAGAGACACTTGAACGATGCACCAGACTCGCTTCTGCTCTTTCCCAGCTCGGAATTTCTCGTGGAGATGTGGTAGCTGCATTGGCTCCAAATATCCCAGCAATGTATGAGCTCCATTTCGGTGTTCCAATGGCAGGAGCAGTTCTCTGTACACTTAATGTACGCCATGATTCACAAATGGTGTCAGTATTATTAAAACATTCTGATGCCAAATTCATTTTTGTAGACTACCAACTTTTCCATGTTGCTAAAGGAGCATTTGACATCCTGTCCAAGACTAGAACCAAGCTACCTGTTCTAGTCTTAATCCCAGAGAGTGATCAATCGTCCCCTGAGTTATGCAACCCCATTTCTGGGAACTTGGAATATGAGAGCCTATTAGGAAGGGGAAAACTCGATTTTGAGATCCGACGGCCAAAAGACGAATGGGATCCAATTTCACTCAACTACACTTCAGGTACTACATCAAGCCCAAAAGGTGTCATTTATAGCCACAGAGGTGCCTATCTCAATTCATTTGCAGCAGTTCTTCTCAATGAGATGCCCTCAATGCCTGTGTATTTATGGTGTGTGCCCATGTTTCATTGCAATGGCTGGTGCCTCACTTGGGCCGTGGCTGCTCAGGGTGGCACCAATATCTGCCAAAGAAATGTCACTGCAAAAGGAATATTTAGCTGTATTTCTCAGCACAAGGTGACCCACATGGGTGGTGCACCCACCGTTTTAAACATGATTGTAAATGCACCAGAAAATGAGCGAAGGCCACTTCCGGGCAAGGTAATAGTCATGACTGGAGGTGCACCGCCACCTGCCCAGGTACTATTCAAGATGGAAGAACTAGGGTTCAATGTAACCCACTCATATGGTTTGACAGAAACTTATGGTCCTGGGACAGTCTGCAGTTGGAAACCTGAATGGGATTCCCTTCCTCGGGTTGAACAGGCAAAGATCAAGTCTAGACAGGGGTTGCAACATCTTGGCATGGAGGAACTCGATGTTAAAGATCCTGTGACCATGAAGAGTGTACCATCTGATGCAAAAACCATGGGTGAGGTTATGTTCAGGGGCAACACCGTGATGAATGGATACTTGAAAGATCATCAAGCAACAAGGGATGCATTTAAAGGTGGATGGTTTCATAGTGGGGACTTGGGGGTGAAACACCCGGATGGTTACATAGAACTAAAGGACCGTTCGAAGGATATTATCATTTCTGGGGGCGAAAATATTAGCACAATTGAGGTGGAATCAGTGCTTTTTAGTCATCCAGATATTCTTGAGGCAGCTATTGTGGGAAGGCCTGATGAATATTGGGGGGAGACACCTTGTGCATTTGTGAAGTTGAAGGATGGCTGTAAAACTGGTAGAGAGGAGATTATTAAGTTTTGTAAGGATCGGTTACCCCACTATATGGCTCCTCGAACAGTTGTTTTTGAAGATCTGCCAAAGACTTCGACCGGGAAGGTGCAGAAGTTTGTATTGAGAGAAAAGGCTAAGGCCATGGGAAGCCTCACCAAGAACACCATCAGCAAACTGTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005777 GO:0042579 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0044424 GO:0044444 GO:0044464
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
AMP-binding AMP-binding_C

Protein Analysis

555

Amino Acids

61.37

Weight (kDa)

8.29

Isoelectric Point (pI)

34.5

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0014332)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G20560 AT1G20560
fragaria_vesca FvH4_2g13610 FvH4_2g13610 FvH4_2g13610 FvH4_2g13610
malus_domestica MD05G1027100.v1.1
prunus_persica Prupe.8G032600_v2.0.a1 Prupe.8G032600_v2.0.a1
pyrus_communis pycom05g01910
rosa_chinensis RchiOBHm_Chr6g0275221
rosa_laevigata RLG00000013486
rosa_roxburghii Rroxscaffold_7G00193540
rosa_rugosa Rorug06G0090300
rosa_samantha Rh6AG203600 Rh6CG209000
rosa_wichuraiana Rw6G017670

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 943
Acc36I ACCTGC 1 cut(s) 943
AccB1I GGYRCC 3 cut(s) 626, 723, 755
AccB7I CCANNNNNTGG 1 cut(s) 1537
AccI GTMKAC 3 cut(s) 78, 102, 351
AciI CCGC 2 cut(s) 24, 929
AclWI GGATC 5 cut(s) 526, 551, 564, 1124, 1527
AcoI YGGCCR 2 cut(s) 69, 539
AcsI RAATTY 2 cut(s) 180, 338
AcuI CTGAAG 1 cut(s) 564
AdeI CACNNNGTG 2 cut(s) 820, 1454
AfaI GTAC 5 cut(s) 277, 288, 586, 945, 1152
AfeI AGCGCT 1 cut(s) 65
AfiI CCNNNNNNNGG 9 cut(s) 260, 479, 480, 733, 896, 1056, 1057, 1324, 1537
AgsI TTSAA 7 cut(s) 140, 952, 973, 1064, 1219, 1471, 1562
AhdI GACNNNNNGTC 1 cut(s) 394
AjnI CCWGG 3 cut(s) 57, 939, 1012
AleI CACNNNNGTG 2 cut(s) 624, 831
AluBI AGCT 7 cut(s) 54, 175, 203, 238, 415, 801, 1412
AluI AGCT 7 cut(s) 54, 175, 203, 238, 415, 801, 1412
Alw21I GWGCWC 3 cut(s) 240, 841, 928
Alw26I GTCTC 3 cut(s) 78, 125, 1439
Alw44I GTGCAC 2 cut(s) 837, 924
AlwI GGATC 5 cut(s) 526, 551, 564, 1124, 1527
Ama87I CYCGRG 1 cut(s) 1056
Aor51HI AGCGCT 1 cut(s) 65
AoxI GGCC 7 cut(s) 69, 89, 539, 735, 887, 1424, 1623
ApaLI GTGCAC 2 cut(s) 837, 924
ApeKI GCWGC 4 cut(s) 203, 647, 743, 1409
ApoI RAATTY 2 cut(s) 180, 338
ArsI GACNNNNNNTTYG 2 cut(s) 1064, 1096
AspLEI GCGC 1 cut(s) 66
AspS9I GGNCC 3 cut(s) 735, 1010, 1315
AsuC2I CCSGG 3 cut(s) 897, 1292, 1585
AsuHPI GGTGA 4 cut(s) 832, 1187, 1294, 1630
AsuII TTCGAA 1 cut(s) 1322
AvaI CYCGRG 1 cut(s) 1056
AvaII GGWCC 2 cut(s) 1010, 1315
BaeGI GKGCMC 3 cut(s) 702, 841, 928
BamHI GGATCC 1 cut(s) 556
BanI GGYRCC 3 cut(s) 626, 723, 755
BanII GRGCYC 1 cut(s) 240
BauI CACGAG 1 cut(s) 186
Bbv12I GWGCWC 3 cut(s) 240, 841, 928
BbvI GCAGC 4 cut(s) 190, 659, 730, 1421
BccI CCATC 6 cut(s) 949, 1162, 1251, 1289, 1469, 1658
BceAI ACGGC 2 cut(s) 554, 722
BcgI CGANNNNNNTGC 2 cut(s) 136, 170
BciT130I CCWGG 3 cut(s) 59, 941, 1014
BciVI GTATCC 1 cut(s) 1205
BclI TGATCA 1 cut(s) 445
BcnI CCSGG 3 cut(s) 897, 1292, 1585
BcoDI GTCTC 3 cut(s) 78, 125, 1439
BfaI CTAG 4 cut(s) 405, 425, 965, 1083
BfmI CTRYAG 2 cut(s) 79, 1024
BfoI RGCGCY 1 cut(s) 67
BfuAI ACCTGC 1 cut(s) 943
BfuI GTATCC 1 cut(s) 1205
BglII AGATCT 1 cut(s) 1564
BisI GCNGC 4 cut(s) 204, 648, 744, 1410
BlsI GCNGC 4 cut(s) 205, 649, 745, 1411
Bme1390I CCNGG 6 cut(s) 59, 897, 941, 1014, 1292, 1585
Bme18I GGWCC 2 cut(s) 1010, 1315
BmeRI GACNNNNNGTC 1 cut(s) 394
BmeT110I CYCGRG 1 cut(s) 1056
BmgT120I GGNCC 3 cut(s) 735, 1010, 1315
BmiI GGNNCC 6 cut(s) 213, 558, 628, 725, 757, 1542
BmrFI CCNGG 6 cut(s) 59, 897, 941, 1014, 1292, 1585
BmsI GCATC 6 cut(s) 120, 134, 322, 657, 1150, 1231
BplI GAGNNNNNCTC 4 cut(s) 255, 287, 656, 688
BpmI CTGGAG 2 cut(s) 80, 939
Bpu10I CCTNAGC 2 cut(s) 747, 1620
Bpu14I TTCGAA 1 cut(s) 1322
BpuEI CTTGAG 1 cut(s) 1424
BpuMI CCSGG 3 cut(s) 897, 1292, 1585
BsaJI CCNNGG 6 cut(s) 738, 939, 1013, 1055, 1170, 1626
BsaXI ACNNNNNCTCC 2 cut(s) 183, 213
Bsc4I CCNNNNNNNGG 9 cut(s) 260, 479, 480, 733, 896, 1056, 1057, 1324, 1537
Bse1I ACTGG 2 cut(s) 922, 1492
Bse3DI GCAATG 3 cut(s) 234, 709, 721
BseBI CCWGG 3 cut(s) 59, 941, 1014
BseDI CCNNGG 6 cut(s) 738, 939, 1013, 1055, 1170, 1626
BseGI GGATG 7 cut(s) 33, 390, 1246, 1262, 1300, 1390, 1480
BseLI CCNNNNNNNGG 9 cut(s) 260, 479, 480, 733, 896, 1056, 1057, 1324, 1537
BseMI GCAATG 3 cut(s) 234, 709, 721
BseMII CTCAG 3 cut(s) 450, 761, 824
BseNI ACTGG 2 cut(s) 922, 1492
BseRI GAGGAG 2 cut(s) 1511, 1533
BseSI GKGCMC 3 cut(s) 702, 841, 928
BseXI GCAGC 4 cut(s) 190, 659, 730, 1421
BseYI CCCAGC 2 cut(s) 171, 223
BsgI GTGCAG 1 cut(s) 1613
Bsh1285I CGRYCG 1 cut(s) 1584
BshFI GGCC 7 cut(s) 71, 91, 541, 737, 889, 1426, 1625
BshNI GGYRCC 3 cut(s) 626, 723, 755
BsiEI CGRYCG 1 cut(s) 1584
BsiHKAI GWGCWC 3 cut(s) 240, 841, 928
BsiHKCI CYCGRG 1 cut(s) 1056
BsiSI CCGG 3 cut(s) 896, 1292, 1584
BslFI GGGAC 3 cut(s) 439, 1030, 1286
BslI CCNNNNNNNGG 9 cut(s) 260, 479, 480, 733, 896, 1056, 1057, 1324, 1537
BsmAI GTCTC 3 cut(s) 78, 125, 1439
BsmFI GGGAC 3 cut(s) 439, 1030, 1286
BsnI GGCC 7 cut(s) 71, 91, 541, 737, 889, 1426, 1625
BsoBI CYCGRG 1 cut(s) 1056
Bsp119I TTCGAA 1 cut(s) 1322
Bsp1286I GDGCHC 4 cut(s) 240, 702, 841, 928
Bsp1407I TGTACA 1 cut(s) 275
Bsp143I GATC 9 cut(s) 12, 445, 531, 556, 1074, 1129, 1222, 1519, 1564
Bsp19I CCATGG 2 cut(s) 1170, 1626
BspACI CCGC 2 cut(s) 24, 929
BspANI GGCC 7 cut(s) 71, 91, 541, 737, 889, 1426, 1625
BspCNI CTCAG 3 cut(s) 451, 760, 823
BspHI TCATGA 1 cut(s) 912
BspLI GGNNCC 6 cut(s) 213, 558, 628, 725, 757, 1542
BspMAI CTGCAG 1 cut(s) 1028
BspMI ACCTGC 1 cut(s) 943
BspPI GGATC 5 cut(s) 526, 551, 564, 1124, 1527
BspT104I TTCGAA 1 cut(s) 1322
BspT107I GGYRCC 3 cut(s) 626, 723, 755
BsrDI GCAATG 3 cut(s) 234, 709, 721
BsrGI TGTACA 1 cut(s) 275
BsrI ACTGG 2 cut(s) 922, 1492
BssECI CCNNGG 6 cut(s) 738, 939, 1013, 1055, 1170, 1626
BssMI GATC 9 cut(s) 12, 445, 531, 556, 1074, 1129, 1222, 1519, 1564
BssNAI GTATAC 1 cut(s) 103
BssSI CACGAG 1 cut(s) 186
BssT1I CCWWGG 2 cut(s) 1170, 1626
Bst1107I GTATAC 1 cut(s) 103
Bst2BI CACGAG 1 cut(s) 186
Bst2UI CCWGG 3 cut(s) 59, 941, 1014
Bst4CI ACNGT 6 cut(s) 847, 1021, 1201, 1319, 1552, 1662
Bst6I CTCTTC 1 cut(s) 1139
BstAUI TGTACA 1 cut(s) 275
BstBI TTCGAA 1 cut(s) 1322
BstDEI CTNAG 4 cut(s) 459, 747, 810, 1620
BstDSI CCRYGG 3 cut(s) 738, 1170, 1626
BstEII GGTNACC 2 cut(s) 820, 1524
BstF5I GGATG 7 cut(s) 33, 390, 1246, 1262, 1300, 1390, 1480
BstH2I RGCGCY 1 cut(s) 67
BstHHI GCGC 1 cut(s) 66
BstKTI GATC 9 cut(s) 15, 448, 534, 559, 1077, 1132, 1225, 1522, 1567
BstMAI GTCTC 3 cut(s) 78, 125, 1439
BstMBI GATC 9 cut(s) 12, 445, 531, 556, 1074, 1129, 1222, 1519, 1564
BstMCI CGRYCG 1 cut(s) 1584
BstMWI GCNNNNNNNGC 4 cut(s) 209, 380, 676, 743
BstNI CCWGG 3 cut(s) 59, 941, 1014
BstPI GGTNACC 2 cut(s) 820, 1524
BstSCI CCNGG 6 cut(s) 57, 895, 939, 1012, 1290, 1583
BstSFI CTRYAG 2 cut(s) 79, 1024
BstSLI GKGCMC 3 cut(s) 702, 841, 928
BstV1I GCAGC 4 cut(s) 190, 659, 730, 1421
BstX2I RGATCY 4 cut(s) 531, 556, 1129, 1564
BstXI CCANNNNNNTGG 2 cut(s) 833, 989
BstYI RGATCY 4 cut(s) 531, 556, 1129, 1564
BstZ17I GTATAC 1 cut(s) 103
BsuI GTATCC 1 cut(s) 1205
BsuRI GGCC 7 cut(s) 71, 91, 541, 737, 889, 1426, 1625
BtgI CCRYGG 3 cut(s) 738, 1170, 1626
BtsCI GGATG 7 cut(s) 33, 390, 1246, 1262, 1300, 1390, 1480
BtsI GCAGTG 1 cut(s) 780
BtsIMutI CAGTG 2 cut(s) 780, 1383
BveI ACCTGC 1 cut(s) 943
CciI TCATGA 1 cut(s) 912
CfoI GCGC 1 cut(s) 66
Cfr13I GGNCC 3 cut(s) 735, 1010, 1315
Csp6I GTAC 5 cut(s) 276, 287, 585, 944, 1151
CspCI CAANNNNNGTGG 2 cut(s) 1350, 1385
CviQI GTAC 5 cut(s) 276, 287, 585, 944, 1151
DdeI CTNAG 4 cut(s) 459, 747, 810, 1620
DpnI GATC 9 cut(s) 14, 447, 533, 558, 1076, 1131, 1224, 1521, 1566
DpnII GATC 9 cut(s) 12, 445, 531, 556, 1074, 1129, 1222, 1519, 1564
DraI TTTAAA 2 cut(s) 852, 1249
DraIII CACNNNGTG 2 cut(s) 820, 1454
DriI GACNNNNNGTC 1 cut(s) 394
EaeI YGGCCR 2 cut(s) 69, 539
Eam1104I CTCTTC 1 cut(s) 1139
Eam1105I GACNNNNNGTC 1 cut(s) 394
EarI CTCTTC 1 cut(s) 1139
Ecl136II GAGCTC 1 cut(s) 238
Eco130I CCWWGG 2 cut(s) 1170, 1626
Eco147I AGGCCT 2 cut(s) 91, 1426
Eco24I GRGCYC 1 cut(s) 240
Eco47I GGWCC 2 cut(s) 1010, 1315
Eco47III AGCGCT 1 cut(s) 65
Eco53kI GAGCTC 1 cut(s) 238
Eco57I CTGAAG 1 cut(s) 564
Eco88I CYCGRG 1 cut(s) 1056
Eco91I GGTNACC 2 cut(s) 820, 1524
EcoICRI GAGCTC 1 cut(s) 238
EcoO65I GGTNACC 2 cut(s) 820, 1524
EcoRII CCWGG 3 cut(s) 57, 939, 1012
EcoT14I CCWWGG 2 cut(s) 1170, 1626
EcoT22I ATGCAT 1 cut(s) 1246
EcoT38I GRGCYC 1 cut(s) 240
ErhI CCWWGG 2 cut(s) 1170, 1626
FaqI GGGAC 3 cut(s) 439, 1030, 1286
FauNDI CATATG 1 cut(s) 988
FbaI TGATCA 1 cut(s) 445
FblI GTMKAC 3 cut(s) 78, 102, 351
Fnu4HI GCNGC 4 cut(s) 204, 648, 744, 1410
FokI GGATG 7 cut(s) 20, 377, 1253, 1269, 1307, 1377, 1487
FriOI GRGCYC 1 cut(s) 240
Fsp4HI GCNGC 4 cut(s) 204, 648, 744, 1410
FspBI CTAG 4 cut(s) 405, 425, 965, 1083
GlaI GCGC 1 cut(s) 65
GluI GCNGC 4 cut(s) 204, 648, 744, 1410
GsaI CCCAGC 2 cut(s) 175, 227
GsuI CTGGAG 2 cut(s) 80, 939
HaeII RGCGCY 1 cut(s) 67
HaeIII GGCC 7 cut(s) 71, 91, 541, 737, 889, 1426, 1625
HapII CCGG 3 cut(s) 896, 1292, 1584
HhaI GCGC 1 cut(s) 66
Hin6I GCGC 1 cut(s) 64
HinP1I GCGC 1 cut(s) 64
HinfI GANTC 4 cut(s) 153, 296, 1046, 1373
HpaII CCGG 3 cut(s) 896, 1292, 1584
HphI GGTGA 4 cut(s) 832, 1187, 1294, 1630
Hpy166II GTNNAC 7 cut(s) 79, 103, 278, 352, 839, 926, 1151
Hpy188I TCNGA 4 cut(s) 179, 331, 536, 1159
Hpy188III TCNNGA 5 cut(s) 913, 952, 1083, 1394, 1403
Hpy8I GTNNAC 7 cut(s) 79, 103, 278, 352, 839, 926, 1151
Hpy99I CGWCG 1 cut(s) 540
HpyAV CCTTC 8 cut(s) 102, 504, 879, 1061, 1318, 1416, 1465, 1582
HpyCH4III ACNGT 6 cut(s) 847, 1021, 1201, 1319, 1552, 1662
HpyF10VI GCNNNNNNNGC 4 cut(s) 209, 380, 676, 743
HpyF3I CTNAG 4 cut(s) 459, 747, 810, 1620
HspAI GCGC 1 cut(s) 64
Ksp22I TGATCA 1 cut(s) 445
Kzo9I GATC 9 cut(s) 12, 445, 531, 556, 1074, 1129, 1222, 1519, 1564
LmnI GCTCC 6 cut(s) 61, 217, 243, 263, 380, 1546
Lsp1109I GCAGC 4 cut(s) 190, 659, 730, 1421
LweI GCATC 6 cut(s) 120, 134, 322, 657, 1150, 1231
MaeI CTAG 4 cut(s) 405, 425, 965, 1083
MaeIII GTNAC 6 cut(s) 778, 820, 976, 1135, 1298, 1524
MalI GATC 9 cut(s) 14, 447, 533, 558, 1076, 1131, 1224, 1521, 1566
MboI GATC 9 cut(s) 12, 445, 531, 556, 1074, 1129, 1222, 1519, 1564
MboII GAAGA 4 cut(s) 647, 971, 1156, 1574
MfeI CAATTG 1 cut(s) 1362
MflI RGATCY 4 cut(s) 531, 556, 1129, 1564
MhlI GDGCHC 4 cut(s) 240, 702, 841, 928
MluCI AATT 5 cut(s) 180, 338, 561, 637, 1362
MlyI GAGTC 1 cut(s) 147
MmeI TCCRAC 2 cut(s) 559, 1010
Mph1103I ATGCAT 1 cut(s) 1246
MseI TTAA 7 cut(s) 282, 320, 431, 851, 1125, 1248, 1506
MslI CAYNNNNRTG 8 cut(s) 246, 330, 624, 714, 831, 987, 1202, 1293
MspI CCGG 3 cut(s) 896, 1292, 1584
MspR9I CCNGG 6 cut(s) 59, 897, 941, 1014, 1292, 1585
MunI CAATTG 1 cut(s) 1362
MvaI CCWGG 3 cut(s) 59, 941, 1014
MwoI GCNNNNNNNGC 4 cut(s) 209, 380, 676, 743
NciI CCSGG 3 cut(s) 897, 1292, 1585
NcoI CCATGG 2 cut(s) 1170, 1626
NdeI CATATG 1 cut(s) 988
NdeII GATC 9 cut(s) 12, 445, 531, 556, 1074, 1129, 1222, 1519, 1564
NlaIV GGNNCC 6 cut(s) 213, 558, 628, 725, 757, 1542
NmeAIII GCCGAG 1 cut(s) 47
NmuCI GTSAC 3 cut(s) 778, 820, 1135
NsiI ATGCAT 1 cut(s) 1246
NspV TTCGAA 1 cut(s) 1322
OliI CACNNNNGTG 2 cut(s) 624, 831
PagI TCATGA 1 cut(s) 912
PaqCI CACCTGC 1 cut(s) 943
PceI AGGCCT 2 cut(s) 91, 1426
PfeI GAWTC 3 cut(s) 296, 1046, 1373
PflMI CCANNNNNTGG 1 cut(s) 1537
PfoI TCCNGGA 1 cut(s) 57
PkrI GCNGC 4 cut(s) 205, 649, 745, 1411
PleI GAGTC 1 cut(s) 147
PpsI GAGTC 1 cut(s) 147
Psp124BI GAGCTC 1 cut(s) 240
Psp6I CCWGG 3 cut(s) 57, 939, 1012
PspEI GGTNACC 2 cut(s) 820, 1524
PspFI CCCAGC 2 cut(s) 171, 223
PspGI CCWGG 3 cut(s) 57, 939, 1012
PspN4I GGNNCC 6 cut(s) 213, 558, 628, 725, 757, 1542
PspPI GGNCC 3 cut(s) 735, 1010, 1315
PstI CTGCAG 1 cut(s) 1028
PsuI RGATCY 4 cut(s) 531, 556, 1129, 1564
RsaI GTAC 5 cut(s) 277, 288, 586, 945, 1152
RsaNI GTAC 5 cut(s) 276, 287, 585, 944, 1151
RseI CAYNNNNRTG 8 cut(s) 246, 330, 624, 714, 831, 987, 1202, 1293
SacI GAGCTC 1 cut(s) 240
SaqAI TTAA 7 cut(s) 282, 320, 431, 851, 1125, 1248, 1506
SatI GCNGC 4 cut(s) 204, 648, 744, 1410
Sau3AI GATC 9 cut(s) 12, 445, 531, 556, 1074, 1129, 1222, 1519, 1564
Sau96I GGNCC 3 cut(s) 735, 1010, 1315
SchI GAGTC 1 cut(s) 147
ScrFI CCNGG 6 cut(s) 59, 897, 941, 1014, 1292, 1585
SduI GDGCHC 4 cut(s) 240, 702, 841, 928
SfaNI GCATC 6 cut(s) 120, 134, 322, 657, 1150, 1231
SfcI CTRYAG 2 cut(s) 79, 1024
SfuI TTCGAA 1 cut(s) 1322
SinI GGWCC 2 cut(s) 1010, 1315
SmiMI CAYNNNNRTG 8 cut(s) 246, 330, 624, 714, 831, 987, 1202, 1293
SmlI CTYRAG 1 cut(s) 1403
SmoI CTYRAG 1 cut(s) 1403
Sse9I AATT 5 cut(s) 180, 338, 561, 637, 1362
SseBI AGGCCT 2 cut(s) 91, 1426
SsiI CCGC 2 cut(s) 24, 929
SspI AATATT 3 cut(s) 795, 1354, 1436
SspMI CTAG 4 cut(s) 405, 425, 965, 1083
SstI GAGCTC 1 cut(s) 240
StuI AGGCCT 2 cut(s) 91, 1426
StyD4I CCNGG 6 cut(s) 57, 895, 939, 1012, 1290, 1583
StyI CCWWGG 2 cut(s) 1170, 1626
TaaI ACNGT 6 cut(s) 847, 1021, 1201, 1319, 1552, 1662
TaqI TCGA 6 cut(s) 11, 522, 1119, 1322, 1546, 1580
TasI AATT 5 cut(s) 180, 338, 561, 637, 1362
TatI WGTACW 1 cut(s) 275
TfiI GAWTC 3 cut(s) 296, 1046, 1373
Tru1I TTAA 7 cut(s) 282, 320, 431, 851, 1125, 1248, 1506
Tru9I TTAA 7 cut(s) 282, 320, 431, 851, 1125, 1248, 1506
TscAI CASTG 2 cut(s) 787, 1383
TseFI GTSAC 3 cut(s) 778, 820, 1135
TseI GCWGC 4 cut(s) 203, 647, 743, 1409
Tsp45I GTSAC 3 cut(s) 778, 820, 1135
TspDTI ATGAA 7 cut(s) 331, 630, 698, 1157, 1220, 1253, 1446
TspGWI ACGGA 1 cut(s) 120
TspRI CASTG 2 cut(s) 787, 1383
Van91I CCANNNNNTGG 1 cut(s) 1537
VneI GTGCAC 2 cut(s) 837, 924
VpaK11BI GGWCC 2 cut(s) 1010, 1315
XapI RAATTY 2 cut(s) 180, 338
XbaI TCTAGA 1 cut(s) 1082
XcmI CCANNNNNNNNNTGG 1 cut(s) 549
XmiI GTMKAC 3 cut(s) 78, 102, 351
XspI CTAG 4 cut(s) 405, 425, 965, 1083
Zsp2I ATGCAT 1 cut(s) 1246
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.