Rorug06G0090300

acyl-activating enzyme 1

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_rugosa
GWHBQTZ00000006
OG0014332
Physical Location & Seq
Reverse (-)
12002101 .. 12012656
10556 bp
Loading structure...
UTR
Exon/CDS
Intron
Rorug06G0090300.1

Sequence Viewer

Length: 3450 bp
ATGGATCTGCGCTGGTCCCTTCTTGGCCTGGTGTGGTCTCTTCTTGGCCTGGTGGCCTTTGCTTTATTTGTGAAAATTACTAGGTCCAAGGATCGACCGGCATCACCAAACAACAAGAACAACATGGATCTGAGCAAACCTCAAGTCGGAGCCTCTTCCTCTTCCTCTTCTTCTTCCACCCATTCGTTCACACAGGATGTCTTTCTGAGCTTCAGAGGTGAGGATACGCGCAACAATTTCACAGGCCATTTGCACAGCAATTTGGTTAACAGGAGAATTAACACCTTCATAGATAATGAGCTTACAAGAGGAGAAGATATAAGAAAGGAGCTCCTGGAAGTAATTGAAGGATCAAGGATTTCCATTGTTGTATTTTCTGCCAACTACGCATCTTCAAAGTGGTGCTTGGATGAACTTGTCAAAATCTTTCAATGTAAAGAATCCAAGCAACAAATCGTTTACCCAATATTCTACAAGGTGGATCCGTCGGATATACGACACCAGAAGGGCCAGGTTGGTGCTCACCTTAGCAAATACAAGGATAACAAGAAGGTGGAGAGTTGGAAGGAAGCTCTTACACAAGCAGCAACTTTGTCTGGGTGGCACATCTCGGATGGAAGGAATGAAGCGAATGTCATTGATGAAATTGTTAGAGAGATATCACCCAAAATAATGAAATGCATCCCTTTAGATGTGGCAACATATCCTGTTGGAATAGAATCTCGTGTAGAAGACATACTTAAGCTCTTAAATGTAGGGCAAGCCGATCCTCACATGGTAGGGATATGGGGAATTGGCGGAATAGGAAAGTCAACACTTGCGAAAGCTGTTTACAATTCAATTAGCAATAAGTTTGAACATAGCTGTTTCCTGGCAAATGTTAGAGAGGAATCATCTTCATCTGGAGGTCTTGTCAAGCTACAAAACAATTTTCTTAAGAATATAGATAGGGATCCACCTCATGTGGAAAATGTTGCTAAAGGAATCACTCTACTGAAGCAAAAACTAAGCCAGAAAAAGGTTCTCTTAGTTATTGATGATGTGGACTGCTTGGGTCAGTTAGAAGCACTTGCTGCAGAGTCTGGTTGGTTTGGTCCAGGAAGTAAAATTATCATAACAACAAGAGATACAAATTGTGTAAGTCCTGATTCAACATATGAGGTCAAGGAATTAAATCATGAAGATGCTTCAAAGCTCTTCAATTGCTATGCCTTCAAAGGAGATATATGTCCGGATGGCTTCTTTGAACTTGCAACTGAGGTAATACATTATGCTAAAGGGATTCCGTTAGTTTTAGAAGTTTTGGGGTCAGACCTACGTAGTAAAAAGGATAAGGATGTGTGGAAAGCTGCATTAGAGTATTACAGGAGATGTCCTAACAAAGATATTCAAAAAATTCTCCAAAGAAGTTACGATGGATTGCATGATCAGATACAAGAAGTTTTTCTTCATATTGCATGTTTCTTTAAAGGTCTTAAAAAATGTTATGTGATTGATGTACTGCAAAGCTGTGACCTGAATCCCAAGTATGCTCTCGAAGTCCTCGTAGAAAAGGCCTTAATAAAAATTGTGAAAGACAATTGGATTTGGATGCATGATCTTATAGAGGACATGGGAAAAGAAATTGTTCGGCAAGAGTCTCCTGAGATAGGAAAACGTAGTAGACTGTGGCTTCATGAGGATGTTAGCGAGGTCCTTGAAGAAAACACAGGAACGGATAAAATTAAAGCCATCGTGTTGAGAAGAGGAGCTTGGAATTTACAAAGCATACAGTTGGACAGCGGAAGCTTCACAAACTTGAAAAATCTTCAAATTTTTAAAGTCACTTATAAGATGCTTAGTGGAGAGAGCGTGGATTATCTCCCCAACCAGTTGAGGGTCCTTGACTGGAGTGGTTGTTCATTACGATCGCTGCCAACGAATTTTAATCCGAGGAAACTTGGCATGCTGCGAATGAGTGGGCCCTGCACGGCTCCTCTGGGATTGAAATTGAAGGAAATGCGACACTTGAAATCTATCAAATTGCGGAACTGTGATGGTCTAACAAGAATTCCCGACTTGTCTGGATTACCATGCTTAAAGGAGCTGGAGCTGTCTTACTGTGAAGATTTAGATGAAGTTGACCCTTCGGCTGGCCGTCTCGATAAGCTCGTCCATTTGGAACTCAGTAATTGTAAAAAGCTCCAGATGTTTCCGAAGGTGATCAACTTGAAATCTTTGGAAACTCTGAAGCTCTCGGATTGGCCGCTTGAGTCCTTCCCTGAAATTGAGGGAGACATGAAGTCTTTGAATCGCCTGGATCTAAGTGGGACTGCCATCAAAGAGTTACCTTGCTCGGTCGGAAATCTCACTGGCCTTGAATCCTTGTATCTAAAGGAGTGTTATAATCTGACAAATGTACCATCGAGCATTTTCTATGGATTGCAACGTCTGGAGGATCTTAATCTTTCGGGATGCGATAACCTTGTTACATTTCCAACAAAGTCAGAATCACTTCCTCCTCCACTAGTCTTTTCAACTAACCTCACTTCCAGATTACAAGTTAATCTGAATGATTGTTGCTGGGGGCTTGAAGACATTTCAGAATTTCCACGAGAAATAGATGGCTTATCTGTGAGTGGTTGTCGGTCATTGAAAACAATTTCAAAGTTGTCAAAAATTTTGGAGGGGAAAGATTCAAAAATGTTCAGCAAGTTGGACTTGACTTGGTGCGACACACTCTGCGGCAATCTGGCTCGTGGAGTGATATCAGGGGTTGACAAGCGACTATTTCATCTGGCCAACAAGAGAACCGGAGGATTGATGGAAGAGGCAGAGGAAGAATTGATGGAAGAGGAAGGGGCAGAGGCAGAGGAAGAGGCAGAGGAAGAATTGTTGGAAGAGGAAGGGGCAGAGGCAGAGGAAGGATTGATGGAAGAGGCAGAGGAAGAATTGATGGAAGAGGCAGTGAAATTGACGGCTCTGTTGACTCTATTTTTCTCTTGTGCGAAATCTGAAGAATTCCAAGTAACATTTTCTGCACATGCTCCGCTTCCAAACTGGTTCACCTGCTGTCAGGATGTGAATTGGGATGTGAATGGTAAAGTGATAAATAAAGAGCATGAGTTTTGCATTGAAATTCCTCAAAACTCCAATTGGGACAACAAAGGGTTGGCTCTCTGTATTCAATCGTATTCTGATGAGCCTGATTTTCCTCCTGAACTCACTCATTTTGTTTACATCAATGGAATAAAGTTTGATGAGAATCGCATGTATTATTCGAATGAAGTGTGCGTGCATTATATTCCATTCGTGACAGTAAAAAAGAGGTTGAGTGAAAGTGGGATATTGTCACCTGATATGTTTCGAGTTATGTTTCGTCTTGAAGAAAATTTTATTGAAACAGATATGCCTTTTGGGGCAAGCTGGGGAGTCCACCTGATCGAAGATTTGGAAGGAGAAGGCCGATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005777 GO:0042579 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0044424 GO:0044444 GO:0044464
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
AMP-binding AMP-binding_C

Protein Analysis

1149

Amino Acids

129.69

Weight (kDa)

5.22

Isoelectric Point (pI)

45.65

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
TIR PF01582 66 - 225 2.2e-53 TIR domain
TIR_2 PF13676 67 - 163 1.7e-13 TIR domain
NB-ARC PF00931 240 - 408 7.8e-17 NB-ARC domain
ATPase_2 PF01637 243 - 354 5.7e-06 ATPase domain predominantly from Archaea
WHD_ROQ1 PF23282 479 - 547 6e-21 Disease resistance protein Roq1-like, winged-helix domain
LRR_14 PF23598 720 - 819 1.6e-09 Leucine-rich repeat region
LRR_13 PF23286 738 - 822 2.7e-08 Disease resistance protein RPS4B-like, leucine-rich repeats
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0014332)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G20560 AT1G20560
fragaria_vesca FvH4_2g13610 FvH4_2g13610 FvH4_2g13610 FvH4_2g13610
malus_domestica MD05G1027100.v1.1
prunus_persica Prupe.8G032600_v2.0.a1 Prupe.8G032600_v2.0.a1
pyrus_communis pycom05g01910
rosa_chinensis RchiOBHm_Chr6g0275221
rosa_laevigata RLG00000013486
rosa_roxburghii Rroxscaffold_7G00193540
rosa_rugosa Rorug06G0090300
rosa_samantha Rh6AG203600 Rh6CG209000
rosa_wichuraiana Rw6G017670

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 2 cut(s) 1830, 2385
AarI CACCTGC 1 cut(s) 3056
Acc36I ACCTGC 1 cut(s) 3056
AccI GTMKAC 1 cut(s) 1663
AccII CGCG 1 cut(s) 229
AccIII TCCGGA 1 cut(s) 1231
AciI CCGC 6 cut(s) 798, 1782, 2026, 2246, 2724, 3029
AcoI YGGCCR 3 cut(s) 2134, 2243, 2778
AcuI CTGAAG 4 cut(s) 196, 1016, 2249, 3015
AfaI GTAC 2 cut(s) 1500, 2400
AfiI CCNNNNNNNGG 5 cut(s) 146, 1018, 1649, 1876, 2132
AflII CTTAAG 2 cut(s) 740, 935
AhdI GACNNNNNGTC 1 cut(s) 2281
AhlI ACTAGT 1 cut(s) 2506
AjnI CCWGG 7 cut(s) 27, 48, 333, 510, 870, 1096, 2295
AjuI GAANNNNNNNTTGG 2 cut(s) 1735, 1767
AloI GAACNNNNNNTCC 2 cut(s) 1882, 1914
Alw21I GWGCWC 2 cut(s) 333, 523
Alw26I GTCTC 4 cut(s) 42, 1644, 2144, 2268
AlwNI CAGNNNCTG 1 cut(s) 1082
Aor13HI TCCGGA 1 cut(s) 1231
ApaI GGGCCC 1 cut(s) 1965
ApeKI GCWGC 5 cut(s) 584, 1073, 1349, 1912, 1948
ArsI GACNNNNNNTTYG 2 cut(s) 129, 161
Asp700I GAANNNNTTC 3 cut(s) 1443, 1626, 2493
AspLEI GCGC 2 cut(s) 12, 231
AspS9I GGNCC 8 cut(s) 15, 84, 508, 1094, 1693, 1879, 1961, 1962
AsuHPI GGTGA 7 cut(s) 96, 230, 515, 654, 2212, 3037, 3324
AsuII TTCGAA 1 cut(s) 3260
AvaII GGWCC 5 cut(s) 15, 84, 1094, 1693, 1879
BaeGI GKGCMC 1 cut(s) 1965
BaeI ACNNNNGTAYC 2 cut(s) 2382, 2415
BalI TGGCCA 1 cut(s) 2780
BamHI GGATCC 2 cut(s) 481, 952
BanII GRGCYC 2 cut(s) 333, 1965
BauI CACGAG 3 cut(s) 723, 2592, 2736
BbsI GAAGAC 2 cut(s) 738, 2580
Bbv12I GWGCWC 2 cut(s) 333, 523
BbvI GCAGC 5 cut(s) 596, 1060, 1336, 1899, 1935
BceAI ACGGC 3 cut(s) 1986, 2121, 2973
BciT130I CCWGG 7 cut(s) 29, 50, 335, 512, 872, 1098, 2297
BciVI GTATCC 1 cut(s) 217
BclI TGATCA 2 cut(s) 1426, 2202
BcoDI GTCTC 4 cut(s) 42, 1644, 2144, 2268
BcuI ACTAGT 1 cut(s) 2506
BfaI CTAG 2 cut(s) 81, 2507
BfmI CTRYAG 1 cut(s) 1074
BfrI CTTAAG 2 cut(s) 740, 935
BfuAI ACCTGC 1 cut(s) 3056
BfuI GTATCC 1 cut(s) 217
BisI GCNGC 7 cut(s) 585, 1074, 1350, 1913, 1949, 2246, 2725
BlsI GCNGC 7 cut(s) 586, 1075, 1351, 1914, 1950, 2247, 2726
Bme1390I CCNGG 7 cut(s) 29, 50, 335, 512, 872, 1098, 2297
Bme18I GGWCC 5 cut(s) 15, 84, 1094, 1693, 1879
BmeRI GACNNNNNGTC 1 cut(s) 2281
BmgT120I GGNCC 8 cut(s) 15, 84, 508, 1094, 1693, 1879, 1961, 1962
BmiI GGNNCC 7 cut(s) 17, 151, 483, 954, 1880, 1963, 1974
BmrFI CCNGG 7 cut(s) 29, 50, 335, 512, 872, 1098, 2297
BmsI GCATC 7 cut(s) 110, 398, 690, 1174, 1581, 1824, 2444
BpiI GAAGAC 2 cut(s) 738, 2580
BplI GAGNNNNNCTC 2 cut(s) 124, 156
BpmI CTGGAG 5 cut(s) 924, 1909, 2108, 2168, 2453
Bpu10I CCTNAGC 1 cut(s) 527
Bpu14I TTCGAA 1 cut(s) 3260
BpuEI CTTGAG 2 cut(s) 126, 2270
BsaAI YACGTR 1 cut(s) 1319
BsaBI GATNNNNATC 3 cut(s) 951, 2442, 3243
BsaI GGTCTC 1 cut(s) 42
BsaJI CCNNGG 2 cut(s) 87, 1931
BsaWI WCCGGW 2 cut(s) 1231, 2792
BsaXI ACNNNNNCTCC 6 cut(s) 897, 927, 1836, 1866, 1882, 1912
Bsc4I CCNNNNNNNGG 5 cut(s) 146, 1018, 1649, 1876, 2132
Bse118I RCCGGY 1 cut(s) 97
Bse1I ACTGG 4 cut(s) 1870, 1892, 2356, 3044
Bse8I GATNNNNATC 3 cut(s) 951, 2442, 3243
BseAI TCCGGA 1 cut(s) 1231
BseBI CCWGG 7 cut(s) 29, 50, 335, 512, 872, 1098, 2297
BseDI CCNNGG 2 cut(s) 87, 1931
BseJI GATNNNNATC 3 cut(s) 951, 2442, 3243
BseLI CCNNNNNNNGG 5 cut(s) 146, 1018, 1649, 1876, 2132
BseMII CTCAG 5 cut(s) 122, 197, 1248, 1635, 2179
BseNI ACTGG 4 cut(s) 1870, 1892, 2356, 3044
BseRI GAGGAG 4 cut(s) 324, 1761, 1965, 2490
BseSI GKGCMC 1 cut(s) 1965
BseXI GCAGC 5 cut(s) 596, 1060, 1336, 1899, 1935
BseYI CCCAGC 2 cut(s) 2562, 3405
BsgI GTGCAG 2 cut(s) 1951, 3003
Bsh1236I CGCG 1 cut(s) 229
Bsh1285I CGRYCG 3 cut(s) 98, 1910, 2340
BsiEI CGRYCG 3 cut(s) 98, 1910, 2340
BsiHKAI GWGCWC 2 cut(s) 333, 523
BsiSI CCGG 3 cut(s) 98, 1232, 2793
BslFI GGGAC 2 cut(s) 2323, 3152
BslI CCNNNNNNNGG 5 cut(s) 146, 1018, 1649, 1876, 2132
BsmAI GTCTC 4 cut(s) 42, 1644, 2144, 2268
BsmBI CGTCTC 1 cut(s) 2144
BsmFI GGGAC 2 cut(s) 2323, 3152
Bso31I GGTCTC 1 cut(s) 42
Bsp119I TTCGAA 1 cut(s) 3260
Bsp120I GGGCCC 1 cut(s) 1961
Bsp1286I GDGCHC 3 cut(s) 333, 523, 1965
Bsp13I TCCGGA 1 cut(s) 1231
BspACI CCGC 6 cut(s) 798, 1782, 2026, 2246, 2724, 3029
BspCNI CTCAG 5 cut(s) 123, 198, 1249, 1636, 2178
BspEI TCCGGA 1 cut(s) 1231
BspFNI CGCG 1 cut(s) 229
BspHI TCATGA 2 cut(s) 1177, 1675
BspLI GGNNCC 7 cut(s) 17, 151, 483, 954, 1880, 1963, 1974
BspMAI CTGCAG 1 cut(s) 1078
BspMI ACCTGC 1 cut(s) 3056
BspQI GCTCTTC 1 cut(s) 1202
BspT104I TTCGAA 1 cut(s) 3260
BspTI CTTAAG 2 cut(s) 740, 935
BspTNI GGTCTC 1 cut(s) 42
BsrFI RCCGGY 1 cut(s) 97
BsrI ACTGG 4 cut(s) 1870, 1892, 2356, 3044
BssAI RCCGGY 1 cut(s) 97
BssECI CCNNGG 2 cut(s) 87, 1931
BssSI CACGAG 3 cut(s) 723, 2592, 2736
BssT1I CCWWGG 1 cut(s) 87
Bst2BI CACGAG 3 cut(s) 723, 2592, 2736
Bst2UI CCWGG 7 cut(s) 29, 50, 335, 512, 872, 1098, 2297
Bst4CI ACNGT 5 cut(s) 1668, 1773, 2033, 2102, 3298
BstAFI CTTAAG 2 cut(s) 740, 935
BstAPI GCANNNNNTGC 1 cut(s) 1073
BstBAI YACGTR 1 cut(s) 1319
BstBI TTCGAA 1 cut(s) 3260
BstC8I GCNNGC 5 cut(s) 762, 1946, 2134, 3275, 3403
BstENI CCTNNNNNAGG 1 cut(s) 1647
BstFNI CGCG 1 cut(s) 229
BstHHI GCGC 2 cut(s) 12, 231
BstMAI GTCTC 4 cut(s) 42, 1644, 2144, 2268
BstMCI CGRYCG 3 cut(s) 98, 1910, 2340
BstMWI GCNNNNNNNGC 2 cut(s) 386, 1073
BstNI CCWGG 7 cut(s) 29, 50, 335, 512, 872, 1098, 2297
BstNSI RCATGY 4 cut(s) 1461, 1948, 3026, 3253
BstSCI CCNGG 7 cut(s) 27, 48, 333, 510, 870, 1096, 2295
BstSFI CTRYAG 1 cut(s) 1074
BstSLI GKGCMC 1 cut(s) 1965
BstSNI TACGTA 1 cut(s) 1319
BstUI CGCG 1 cut(s) 229
BstV1I GCAGC 5 cut(s) 596, 1060, 1336, 1899, 1935
BstV2I GAAGAC 2 cut(s) 738, 2580
BstX2I RGATCY 6 cut(s) 4, 127, 481, 952, 2299, 2437
BstYI RGATCY 6 cut(s) 4, 127, 481, 952, 2299, 2437
BsuI GTATCC 1 cut(s) 217
BtsI GCAGTG 1 cut(s) 2952
BtsIMutI CAGTG 2 cut(s) 2349, 2952
BveI ACCTGC 1 cut(s) 3056
Cac8I GCNNGC 5 cut(s) 762, 1946, 2134, 3275, 3403
CaiI CAGNNNCTG 1 cut(s) 1082
CciI TCATGA 2 cut(s) 1177, 1675
CfoI GCGC 2 cut(s) 12, 231
Cfr10I RCCGGY 1 cut(s) 97
Cfr13I GGNCC 8 cut(s) 15, 84, 508, 1094, 1693, 1879, 1961, 1962
Csp6I GTAC 2 cut(s) 1499, 2399
CviQI GTAC 2 cut(s) 1499, 2399
DraI TTTAAA 2 cut(s) 1468, 1819
DriI GACNNNNNGTC 1 cut(s) 2281
EaeI YGGCCR 3 cut(s) 2134, 2243, 2778
Eam1105I GACNNNNNGTC 1 cut(s) 2281
EciI GGCGGA 1 cut(s) 813
Ecl136II GAGCTC 1 cut(s) 331
Eco105I TACGTA 1 cut(s) 1319
Eco130I CCWWGG 1 cut(s) 87
Eco147I AGGCCT 1 cut(s) 1556
Eco24I GRGCYC 2 cut(s) 333, 1965
Eco31I GGTCTC 1 cut(s) 42
Eco32I GATATC 2 cut(s) 660, 2748
Eco47I GGWCC 5 cut(s) 15, 84, 1094, 1693, 1879
Eco53kI GAGCTC 1 cut(s) 331
Eco57I CTGAAG 4 cut(s) 196, 1016, 2249, 3015
EcoICRI GAGCTC 1 cut(s) 331
EcoNI CCTNNNNNAGG 1 cut(s) 1647
EcoO109I RGGNCCY 3 cut(s) 1693, 1879, 1962
EcoRI GAATTC 2 cut(s) 2049, 2999
EcoRII CCWGG 7 cut(s) 27, 48, 333, 510, 870, 1096, 2295
EcoRV GATATC 2 cut(s) 660, 2748
EcoT14I CCWWGG 1 cut(s) 87
EcoT22I ATGCAT 2 cut(s) 683, 1596
EcoT38I GRGCYC 2 cut(s) 333, 1965
ErhI CCWWGG 1 cut(s) 87
Esp3I CGTCTC 1 cut(s) 2144
FaqI GGGAC 2 cut(s) 2323, 3152
FauNDI CATATG 1 cut(s) 1156
FbaI TGATCA 2 cut(s) 1426, 2202
FblI GTMKAC 1 cut(s) 1663
Fnu4HI GCNGC 7 cut(s) 585, 1074, 1350, 1913, 1949, 2246, 2725
FriOI GRGCYC 2 cut(s) 333, 1965
Fsp4HI GCNGC 7 cut(s) 585, 1074, 1350, 1913, 1949, 2246, 2725
FspBI CTAG 2 cut(s) 81, 2507
GlaI GCGC 2 cut(s) 11, 230
GluI GCNGC 7 cut(s) 585, 1074, 1350, 1913, 1949, 2246, 2725
GsaI CCCAGC 2 cut(s) 2566, 3409
GsuI CTGGAG 5 cut(s) 924, 1909, 2108, 2168, 2453
HapII CCGG 3 cut(s) 98, 1232, 2793
HhaI GCGC 2 cut(s) 12, 231
Hin6I GCGC 2 cut(s) 10, 229
HinP1I GCGC 2 cut(s) 10, 229
HincII GTYRAC 5 cut(s) 268, 813, 2122, 2758, 2967
HindII GTYRAC 5 cut(s) 268, 813, 2122, 2758, 2967
HindIII AAGCTT 1 cut(s) 1786
HpaI GTTAAC 1 cut(s) 268
HpaII CCGG 3 cut(s) 98, 1232, 2793
HphI GGTGA 7 cut(s) 96, 230, 515, 654, 2212, 3037, 3324
Hpy99I CGWCG 1 cut(s) 490
HpyCH4III ACNGT 5 cut(s) 1668, 1773, 2033, 2102, 3298
HpyCH4IV ACGT 3 cut(s) 1318, 1657, 2428
HpyF10VI GCNNNNNNNGC 2 cut(s) 386, 1073
HpySE526I ACGT 3 cut(s) 1318, 1657, 2428
HspAI GCGC 2 cut(s) 10, 229
Kpn2I TCCGGA 1 cut(s) 1231
Ksp22I TGATCA 2 cut(s) 1426, 2202
KspAI GTTAAC 1 cut(s) 268
LguI GCTCTTC 1 cut(s) 1202
LmnI GCTCC 9 cut(s) 149, 328, 336, 1748, 1978, 2083, 2089, 2187, 3031
Lsp1109I GCAGC 5 cut(s) 596, 1060, 1336, 1899, 1935
LweI GCATC 7 cut(s) 110, 398, 690, 1174, 1581, 1824, 2444
MaeI CTAG 2 cut(s) 81, 2507
MaeII ACGT 3 cut(s) 1318, 1657, 2428
MaeIII GTNAC 8 cut(s) 1409, 1511, 1822, 2325, 2467, 3007, 3292, 3330
MfeI CAATTG 3 cut(s) 1201, 1579, 3133
MflI RGATCY 6 cut(s) 4, 127, 481, 952, 2299, 2437
MhlI GDGCHC 3 cut(s) 333, 523, 1965
MlsI TGGCCA 1 cut(s) 2780
MluNI TGGCCA 1 cut(s) 2780
MlyI GAGTC 5 cut(s) 1088, 1646, 2261, 2962, 3420
MmeI TCCRAC 9 cut(s) 127, 468, 542, 691, 1755, 2320, 2501, 2676, 2856
Mox20I TGGCCA 1 cut(s) 2780
Mph1103I ATGCAT 2 cut(s) 683, 1596
MroI TCCGGA 1 cut(s) 1231
MroXI GAANNNNTTC 3 cut(s) 1443, 1626, 2493
MscI TGGCCA 1 cut(s) 2780
MslI CAYNNNNRTG 2 cut(s) 1182, 1680
Msp20I TGGCCA 1 cut(s) 2780
MspA1I CMGCKG 1 cut(s) 1782
MspCI CTTAAG 2 cut(s) 740, 935
MspI CCGG 3 cut(s) 98, 1232, 2793
MspR9I CCNGG 7 cut(s) 29, 50, 335, 512, 872, 1098, 2297
MunI CAATTG 3 cut(s) 1201, 1579, 3133
MvaI CCWGG 7 cut(s) 29, 50, 335, 512, 872, 1098, 2297
MvnI CGCG 1 cut(s) 229
MwoI GCNNNNNNNGC 2 cut(s) 386, 1073
NdeI CATATG 1 cut(s) 1156
NlaIV GGNNCC 7 cut(s) 17, 151, 483, 954, 1880, 1963, 1974
NmuCI GTSAC 4 cut(s) 1511, 1822, 3292, 3330
NsiI ATGCAT 2 cut(s) 683, 1596
NspI RCATGY 4 cut(s) 1461, 1948, 3026, 3253
NspV TTCGAA 1 cut(s) 3260
PaeI GCATGC 1 cut(s) 1948
PagI TCATGA 2 cut(s) 1177, 1675
PaqCI CACCTGC 1 cut(s) 3056
PceI AGGCCT 1 cut(s) 1556
PciSI GCTCTTC 1 cut(s) 1202
PcsI WCGNNNNNNNCGW 3 cut(s) 1542, 1916, 2148
PdmI GAANNNNTTC 3 cut(s) 1443, 1626, 2493
PfoI TCCNGGA 2 cut(s) 333, 1096
PkrI GCNGC 7 cut(s) 586, 1075, 1351, 1914, 1950, 2247, 2726
Ple19I CGATCG 1 cut(s) 1910
PleI GAGTC 5 cut(s) 1087, 1645, 2260, 2962, 3419
PpsI GAGTC 5 cut(s) 1087, 1645, 2260, 2962, 3419
Ppu21I YACGTR 1 cut(s) 1319
PpuMI RGGWCCY 2 cut(s) 1693, 1879
PsiI TTATAA 2 cut(s) 1830, 2385
Psp124BI GAGCTC 1 cut(s) 333
Psp5II RGGWCCY 2 cut(s) 1693, 1879
Psp6I CCWGG 7 cut(s) 27, 48, 333, 510, 870, 1096, 2295
PspFI CCCAGC 2 cut(s) 2562, 3405
PspGI CCWGG 7 cut(s) 27, 48, 333, 510, 870, 1096, 2295
PspN4I GGNNCC 7 cut(s) 17, 151, 483, 954, 1880, 1963, 1974
PspOMI GGGCCC 1 cut(s) 1961
PspPI GGNCC 8 cut(s) 15, 84, 508, 1094, 1693, 1879, 1961, 1962
PspPPI RGGWCCY 2 cut(s) 1693, 1879
PstI CTGCAG 1 cut(s) 1078
PstNI CAGNNNCTG 1 cut(s) 1082
PsuI RGATCY 6 cut(s) 4, 127, 481, 952, 2299, 2437
PvuI CGATCG 1 cut(s) 1910
RsaI GTAC 2 cut(s) 1500, 2400
RsaNI GTAC 2 cut(s) 1499, 2399
RseI CAYNNNNRTG 2 cut(s) 1182, 1680
SacI GAGCTC 1 cut(s) 333
SapI GCTCTTC 1 cut(s) 1202
SatI GCNGC 7 cut(s) 585, 1074, 1350, 1913, 1949, 2246, 2725
Sau96I GGNCC 8 cut(s) 15, 84, 508, 1094, 1693, 1879, 1961, 1962
SchI GAGTC 5 cut(s) 1088, 1646, 2261, 2962, 3420
ScrFI CCNGG 7 cut(s) 29, 50, 335, 512, 872, 1098, 2297
SduI GDGCHC 3 cut(s) 333, 523, 1965
SfaNI GCATC 7 cut(s) 110, 398, 690, 1174, 1581, 1824, 2444
SfcI CTRYAG 1 cut(s) 1074
SfuI TTCGAA 1 cut(s) 3260
SinI GGWCC 5 cut(s) 15, 84, 1094, 1693, 1879
SmiMI CAYNNNNRTG 2 cut(s) 1182, 1680
SmlI CTYRAG 4 cut(s) 141, 740, 935, 2249
SmoI CTYRAG 4 cut(s) 141, 740, 935, 2249
SnaBI TACGTA 1 cut(s) 1319
SpeI ACTAGT 1 cut(s) 2506
SphI GCATGC 1 cut(s) 1948
SseBI AGGCCT 1 cut(s) 1556
SsiI CCGC 6 cut(s) 798, 1782, 2026, 2246, 2724, 3029
SspI AATATT 1 cut(s) 468
SspMI CTAG 2 cut(s) 81, 2507
SstI GAGCTC 1 cut(s) 333
StuI AGGCCT 1 cut(s) 1556
StyD4I CCNGG 7 cut(s) 27, 48, 333, 510, 870, 1096, 2295
StyI CCWWGG 1 cut(s) 87
TaaI ACNGT 5 cut(s) 1668, 1773, 2033, 2102, 3298
TaiI ACGT 3 cut(s) 1321, 1660, 2431
TaqI TCGA 7 cut(s) 94, 1536, 2142, 2405, 3260, 3346, 3423
TaqII GACCGA 2 cut(s) 2326, 2616
TatI WGTACW 1 cut(s) 1498
TauI GCSGC 2 cut(s) 2248, 2727
TscAI CASTG 2 cut(s) 2356, 2952
TseFI GTSAC 4 cut(s) 1511, 1822, 3292, 3330
TseI GCWGC 5 cut(s) 584, 1073, 1349, 1912, 1948
Tsp45I GTSAC 4 cut(s) 1511, 1822, 3292, 3330
TspGWI ACGGA 3 cut(s) 474, 1275, 1730
TspRI CASTG 2 cut(s) 2356, 2952
Vha464I CTTAAG 2 cut(s) 740, 935
VpaK11BI GGWCC 5 cut(s) 15, 84, 1094, 1693, 1879
XagI CCTNNNNNAGG 1 cut(s) 1647
XceI RCATGY 4 cut(s) 1461, 1948, 3026, 3253
XmiI GTMKAC 1 cut(s) 1663
XmnI GAANNNNTTC 3 cut(s) 1443, 1626, 2493
XspI CTAG 2 cut(s) 81, 2507
Zsp2I ATGCAT 2 cut(s) 683, 1596
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.