FvH4_2g20610

Pre-mRNA-splicing factor ATP-dependent RNA

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb2
OG0015858
Physical Location & Seq
Forward (+)
17264208 .. 17264800
593 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_2g20610.t1

Sequence Viewer

Length: 312 bp
ATGATGAGTGAATTCCCCTTGGACCCTCAGATATCGAAGATGCTTGTTGTCAGTCCTCAATACAACTGCTCCAATGAGATCTTGACAATTGCTGCCATGCTTTCAGTGCCGCATCCTTTTGTCCGGCCTAGGGCGGATGAGGCTCAAAAAGCCGCTGCAGATGAAGCAAAAGCCAGGTTTGTGCACATTGATGGAGATCACCTCACTATGCTAAATGTGTACCATGCGTTCAAACAAAACAAGGAGGATCCATCCTGGTGCTATGACAATTTTCTAGATCACATGGGCACTAAAGGCTGCAGAAGATGTTAG

Protein Analysis

104

Amino Acids

11.74

Weight (kDa)

6.16

Isoelectric Point (pI)

50.84

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
HA2_C PF21010 8 - 70 9.7e-18 Helicase associated domain (HA2), ratchet-like
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0015858)

Species Orthologous Gene IDs
arabidopsis_thaliana AT5G14900
fragaria_vesca FvH4_2g20600 FvH4_2g20610
malus_domestica MD13G1065100.v1.1
pyrus_communis pycom13g05740
rosa_chinensis RchiOBHm_Chr6g0286781
rosa_laevigata RLG00000012542
rosa_multiflora Rmu_sc0007546.1_g000017
rosa_roxburghii Rroxscaffold_7G00182020
rosa_rugosa Rorug06G0182900
rosa_samantha Rh6AG295500 Rh6BG299600 Rh6CG299400 Rh6DG291300
rosa_wichuraiana Rw6G025440

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AciI CCGC 3 cut(s) 110, 134, 153
AclWI GGATC 2 cut(s) 242, 255
AcsI RAATTY 1 cut(s) 11
AfaI GTAC 1 cut(s) 221
AfiI CCNNNNNNNGG 1 cut(s) 130
AgsI TTSAA 1 cut(s) 232
AjnI CCWGG 2 cut(s) 173, 254
Alw21I GWGCWC 1 cut(s) 186
Alw44I GTGCAC 1 cut(s) 182
AlwI GGATC 2 cut(s) 242, 255
AoxI GGCC 1 cut(s) 125
ApaLI GTGCAC 1 cut(s) 182
ApeKI GCWGC 3 cut(s) 92, 155, 297
ApoI RAATTY 1 cut(s) 11
AspA2I CCTAGG 1 cut(s) 128
AspS9I GGNCC 1 cut(s) 22
AsuHPI GGTGA 1 cut(s) 191
AvaII GGWCC 1 cut(s) 22
AvrII CCTAGG 1 cut(s) 128
BaeGI GKGCMC 2 cut(s) 186, 290
BamHI GGATCC 1 cut(s) 247
Bbv12I GWGCWC 1 cut(s) 186
BbvI GCAGC 3 cut(s) 79, 142, 284
BccI CCATC 2 cut(s) 185, 259
BciT130I CCWGG 2 cut(s) 175, 256
BfaI CTAG 2 cut(s) 129, 275
BfmI CTRYAG 2 cut(s) 156, 298
BglII AGATCT 1 cut(s) 78
BisI GCNGC 5 cut(s) 93, 110, 153, 156, 298
BlnI CCTAGG 1 cut(s) 128
BlsI GCNGC 5 cut(s) 94, 111, 154, 157, 299
Bme1390I CCNGG 2 cut(s) 175, 256
Bme18I GGWCC 1 cut(s) 22
BmgT120I GGNCC 1 cut(s) 22
BmiI GGNNCC 2 cut(s) 24, 249
BmrFI CCNGG 2 cut(s) 175, 256
BmsI GCATC 2 cut(s) 30, 121
BplI GAGNNNNNCTC 2 cut(s) 186, 218
BsaBI GATNNNNATC 1 cut(s) 195
BsaJI CCNNGG 2 cut(s) 18, 128
BsaXI ACNNNNNCTCC 2 cut(s) 53, 83
Bsc4I CCNNNNNNNGG 1 cut(s) 130
Bse8I GATNNNNATC 1 cut(s) 195
BseBI CCWGG 2 cut(s) 175, 256
BseDI CCNNGG 2 cut(s) 18, 128
BseGI GGATG 3 cut(s) 112, 142, 251
BseJI GATNNNNATC 1 cut(s) 195
BseLI CCNNNNNNNGG 1 cut(s) 130
BseMII CTCAG 1 cut(s) 41
BseSI GKGCMC 2 cut(s) 186, 290
BseXI GCAGC 3 cut(s) 79, 142, 284
BshFI GGCC 1 cut(s) 127
BsiHKAI GWGCWC 1 cut(s) 186
BsiSI CCGG 1 cut(s) 124
BslI CCNNNNNNNGG 1 cut(s) 130
BsnI GGCC 1 cut(s) 127
Bsp1286I GDGCHC 2 cut(s) 186, 290
Bsp143I GATC 4 cut(s) 78, 196, 247, 277
BspACI CCGC 3 cut(s) 110, 134, 153
BspANI GGCC 1 cut(s) 127
BspCNI CTCAG 1 cut(s) 40
BspLI GGNNCC 2 cut(s) 24, 249
BspMAI CTGCAG 2 cut(s) 160, 302
BspPI GGATC 2 cut(s) 242, 255
BssECI CCNNGG 2 cut(s) 18, 128
BssMI GATC 4 cut(s) 78, 196, 247, 277
BssT1I CCWWGG 2 cut(s) 18, 128
Bst2UI CCWGG 2 cut(s) 175, 256
BstDEI CTNAG 1 cut(s) 27
BstF5I GGATG 3 cut(s) 112, 142, 251
BstKTI GATC 4 cut(s) 81, 199, 250, 280
BstMBI GATC 4 cut(s) 78, 196, 247, 277
BstMWI GCNNNNNNNGC 5 cut(s) 106, 140, 149, 164, 294
BstNI CCWGG 2 cut(s) 175, 256
BstSCI CCNGG 2 cut(s) 173, 254
BstSFI CTRYAG 2 cut(s) 156, 298
BstSLI GKGCMC 2 cut(s) 186, 290
BstV1I GCAGC 3 cut(s) 79, 142, 284
BstX2I RGATCY 2 cut(s) 78, 247
BstYI RGATCY 2 cut(s) 78, 247
BsuRI GGCC 1 cut(s) 127
BtsCI GGATG 3 cut(s) 112, 142, 251
BtsIMutI CAGTG 1 cut(s) 111
Cfr13I GGNCC 1 cut(s) 22
Csp6I GTAC 1 cut(s) 220
CviAII CATG 3 cut(s) 97, 224, 283
CviJI RGCY 5 cut(s) 127, 143, 152, 173, 297
CviKI_1 RGCY 5 cut(s) 127, 143, 152, 173, 297
CviQI GTAC 1 cut(s) 220
DdeI CTNAG 1 cut(s) 27
DpnI GATC 4 cut(s) 80, 198, 249, 279
DpnII GATC 4 cut(s) 78, 196, 247, 277
EciI GGCGGA 1 cut(s) 149
Eco130I CCWWGG 2 cut(s) 18, 128
Eco32I GATATC 1 cut(s) 33
Eco47I GGWCC 1 cut(s) 22
EcoRI GAATTC 1 cut(s) 11
EcoRII CCWGG 2 cut(s) 173, 254
EcoRV GATATC 1 cut(s) 33
EcoT14I CCWWGG 2 cut(s) 18, 128
ErhI CCWWGG 2 cut(s) 18, 128
FaeI CATG 3 cut(s) 100, 227, 286
FaiI YATR 5 cut(s) 98, 209, 225, 264, 284
FatI CATG 3 cut(s) 96, 223, 282
Fnu4HI GCNGC 5 cut(s) 93, 110, 153, 156, 298
FokI GGATG 3 cut(s) 99, 149, 238
Fsp4HI GCNGC 5 cut(s) 93, 110, 153, 156, 298
FspBI CTAG 2 cut(s) 129, 275
GluI GCNGC 5 cut(s) 93, 110, 153, 156, 298
HaeIII GGCC 1 cut(s) 127
HapII CCGG 1 cut(s) 124
Hin1II CATG 3 cut(s) 100, 227, 286
HpaII CCGG 1 cut(s) 124
HphI GGTGA 1 cut(s) 191
Hpy166II GTNNAC 2 cut(s) 184, 220
Hpy188I TCNGA 1 cut(s) 30
Hpy188III TCNNGA 2 cut(s) 82, 275
Hpy8I GTNNAC 2 cut(s) 184, 220
HpyCH4V TGCA 3 cut(s) 158, 184, 300
HpyF10VI GCNNNNNNNGC 5 cut(s) 106, 140, 149, 164, 294
HpyF3I CTNAG 1 cut(s) 27
Hsp92II CATG 3 cut(s) 100, 227, 286
Kzo9I GATC 4 cut(s) 78, 196, 247, 277
LmnI GCTCC 1 cut(s) 74
LpnPI CCDG 5 cut(s) 137, 160, 187, 241, 268
Lsp1109I GCAGC 3 cut(s) 79, 142, 284
LweI GCATC 2 cut(s) 30, 121
MaeI CTAG 2 cut(s) 129, 275
MalI GATC 4 cut(s) 80, 198, 249, 279
MboI GATC 4 cut(s) 78, 196, 247, 277
MboII GAAGA 1 cut(s) 49
MfeI CAATTG 1 cut(s) 87
MflI RGATCY 2 cut(s) 78, 247
MhlI GDGCHC 2 cut(s) 186, 290
MluCI AATT 3 cut(s) 11, 87, 268
MnlI CCTC 5 cut(s) 36, 66, 133, 212, 238
MslI CAYNNNNRTG 2 cut(s) 189, 256
MspA1I CMGCKG 1 cut(s) 155
MspI CCGG 1 cut(s) 124
MspR9I CCNGG 2 cut(s) 175, 256
MunI CAATTG 1 cut(s) 87
MvaI CCWGG 2 cut(s) 175, 256
MwoI GCNNNNNNNGC 5 cut(s) 106, 140, 149, 164, 294
NdeII GATC 4 cut(s) 78, 196, 247, 277
NlaIII CATG 3 cut(s) 100, 227, 286
NlaIV GGNNCC 2 cut(s) 24, 249
PkrI GCNGC 5 cut(s) 94, 111, 154, 157, 299
Psp6I CCWGG 2 cut(s) 173, 254
PspGI CCWGG 2 cut(s) 173, 254
PspN4I GGNNCC 2 cut(s) 24, 249
PspPI GGNCC 1 cut(s) 22
PsrI GAACNNNNNNTAC 2 cut(s) 212, 244
PstI CTGCAG 2 cut(s) 160, 302
PsuI RGATCY 2 cut(s) 78, 247
RsaI GTAC 1 cut(s) 221
RsaNI GTAC 1 cut(s) 220
RseI CAYNNNNRTG 2 cut(s) 189, 256
SatI GCNGC 5 cut(s) 93, 110, 153, 156, 298
Sau3AI GATC 4 cut(s) 78, 196, 247, 277
Sau96I GGNCC 1 cut(s) 22
ScrFI CCNGG 2 cut(s) 175, 256
SduI GDGCHC 2 cut(s) 186, 290
SetI ASST 2 cut(s) 179, 204
SfaNI GCATC 2 cut(s) 30, 121
SfcI CTRYAG 2 cut(s) 156, 298
SinI GGWCC 1 cut(s) 22
SmiMI CAYNNNNRTG 2 cut(s) 189, 256
Sse9I AATT 3 cut(s) 11, 87, 268
SsiI CCGC 3 cut(s) 110, 134, 153
SspMI CTAG 2 cut(s) 129, 275
StyD4I CCNGG 2 cut(s) 173, 254
StyI CCWWGG 2 cut(s) 18, 128
TaqI TCGA 1 cut(s) 35
TasI AATT 3 cut(s) 11, 87, 268
TauI GCSGC 2 cut(s) 112, 155
TscAI CASTG 1 cut(s) 111
TseI GCWGC 3 cut(s) 92, 155, 297
TspDTI ATGAA 1 cut(s) 177
TspRI CASTG 1 cut(s) 111
VneI GTGCAC 1 cut(s) 182
VpaK11BI GGWCC 1 cut(s) 22
XapI RAATTY 1 cut(s) 11
XbaI TCTAGA 1 cut(s) 274
XmaJI CCTAGG 1 cut(s) 128
XspI CTAG 2 cut(s) 129, 275
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.