Basic Information

Type: gene

Biological Identity

Species

rosa_samantha

Chromosome

Chr6D

Orthogroup ID

OG0015858

Physical Location & Seq

Genomic Coordinates Forward (+)

48917080 .. 48919311

Gene Structure 2232 bp

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UTR

Exon/CDS

Intron

Transcript / Protein ID

Rh6DG291300.1

Sequence Viewer

CDS Protein Promoter (2k)

Length: 2094 bp

ATGGCTAGTTCTATTGTACGTACCAAAAGGAAGAGGAAGCTGTGCTTATTCGACCATTCGGTCTCATCCGACTCCAAGAGGAGCAAACCAAATGGTGGTGGAGGAGCAACCTCAACGAACCTCGATACTAGTATTGATCTCATCAACAACAGGTGGACTGGAAGGCCTTACTCAGAAAGGTACTATGAGATCCTTGAGAAGAGGAGAACTTTGCCTGTCTGGAAATATAAAGAGAAGTTCTTGCAGGAACTCAAGGGCACCCAATGTTTAGTTTTAGTTGGTGAAACCGGCAGTGGTAAAACTACTCAGATTCCTCAATTTGTTTTGGAAGCTGTTGCCGAGAATGTATATCCAGAACTAACACGCAGCAAGAGGAAGATGATGATCGCGTGTACTCAGCCCCGTCGAGTGGCTGCAATGTCAGTGTCCCGCCGTGTTGCTGAGGAGATGGATGTGGTCATCGGAGAAGAGGTTGGTTACAGCATCCGTTTCGAGGAGTGCACCAGCGGGAAGACAATGTTGAAGTACTTAACTGATGGTATGCTTTTAAGAGAAGCAATGGCAGATCCACTTTTTGAGAGATATAATGTCATAATTCTTGACGAAGCTCATGAAAGAACTTTGGCCACTGATCTTCTGTTTGGGATTCTCAAGGAAGTGTTGAAAGTTAGAGCTGACCTGAAGCTGGTTGTGATGAGTGCTACTCTTGAGGCCAAGAAGTTTCGAGATTATTTTAATGGTGCACCCCTCATAGAAGTTCCTGGCAGGCTTTATCCTGTGGAGATTATGTACACTCAGCAACCCGAAAGGGATTACGTAGAGGCAGCAATACGAACTGTTGCACAGATTCTTACTTGTGAGGCTCCTGGAGATGTGCTTGTTTTCCTAACTGGAGGGGAGGAAATAGAAGATGCATGCCTCAAAATTAATAGAGAAATTAATAAAATCAATTACCAGTGTGAAATTGGTCCTGTGAAAGTGATGCCATTGTATTCAACTCTCCCCTCTGATATGCAACAGAAAGTGTTTGATCAAATTCCTGAGAGGAAAATTGTGGTGTCAACAAACATTGCAGAAACTTCTCTTACCATAGATGGAGTTGTTTATGTTGTTGACCCCGGATTTTCCAAACAGAAAGTTTATAACCCACGAGCGCATGTTGAGTCCTTGTTGGTGTCTCCAATTTCTAAGGCAAGTGCACGTCAGAGGTCAGGGCGTGCCGGAAGAACTCAGCCGGGAAAATGCTTTAGACTATACACTCAGAAAAGTTTCCACAACGATCTTGAGCAAGAGACATATCCAGAGATATTGAGATCAAACCTTGCAAATACAGTACTTACCATGAAAAAACTAGGAGTAGAGGATTTGAGTCATTATGATTTCATGGATCCTCCTGCTCCTGAGACAATGATGGATGCACTAGAGGTTTTGTATTTTTTGGGAGCCCTTGATGAAGATGGTAACTTGACCATGGTGGGGGAGATTATGAGTGAATTCCCACTAGACCCTCAGATGTCAAAGATGCTCATTGTCAGCCCTCAATACAACTGCTCCAATGAGATTTTGTCAATTTCTGCGATGCTTTCCGTGCCCACCCCATTTATCCGGCCTAGGGATGAGGTTCAAGAAGCCGACGAAGCCAAAGCTGAGTTTGCCCACATTGACGGAGATCACCTCACTTTGCTCAATGTGTATCATGCTTACAAGCAACACAATGAGGACCCATCCTGGTGCTATGACAACTATCTCAGTCATAAGGCATTGAAGGCTGCAGACAATGTTAGGCAGCAGCTGATGCGCATTATGGCTCGTCTTAACCTCAAGCTATGTAGTACTGACTTAAGCAGCCCTGACTACTACATCAACATAAGAAAGGCTCTGCTGGCTGGATATTTCAGGCAGGTAGCTCATCTACAACGCAGCGGACAGTACCTGACTAGAAAAGAGAAACAAGTAGTACATTTGCATCCCTCAAATTCTCTGGATCACAAACCAGAGTTTGTGATCTACAACGAGTATGTCTTGACAAGCAGGAGTTTTATCCGAACGGTGACCCAGATAAGTGGTGAATGGCTAGTCGAATACAACATCTGA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0003674 GO:0003676 GO:0003723 GO:0003724 GO:0003824 GO:0004004 GO:0004386 GO:0005488 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0005730 GO:0005737 GO:0005829 GO:0006139 GO:0006396 GO:0006725 GO:0006807 GO:0008026 GO:0008150 GO:0008152 GO:0008186 GO:0009507 GO:0009526 GO:0009536 GO:0009941 GO:0009987 GO:0010467 GO:0016020 GO:0016070 GO:0016462 GO:0016787 GO:0016817 GO:0016818 GO:0016887 GO:0017111 GO:0031967 GO:0031974 GO:0031975 GO:0031981 GO:0034641 GO:0042623 GO:0043170 GO:0043226 GO:0043227 GO:0043228 GO:0043229 GO:0043231 GO:0043232 GO:0043233 GO:0044237 GO:0044238 GO:0044422 GO:0044424 GO:0044428 GO:0044434 GO:0044435 GO:0044444 GO:0044446 GO:0044464 GO:0046483 GO:0070013 GO:0070035 GO:0071704 GO:0090304 GO:0097159 GO:0140098 GO:1901360 GO:1901363

KEGG Pathways

Metabolic & Signaling

ko03040 map03040

Pfam Domains

Protein Families

AAA_22 DEAD HA2 Helicase_C OB_NTP_bind

Protein Analysis

697

Amino Acids

79.35

Weight (kDa)

6.44

Isoelectric Point (pI)

49.82

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
DEAD	PF00270	78 - 240	3.5e-08	DEAD/DEAH box helicase
AAA_22	PF13401	86 - 222	5.1e-07	AAA domain
Helicase_C	PF00271	277 - 410	1.3e-10	Helicase conserved C-terminal domain
WHD_HA2	PF04408	473 - 501	1.9e-10	Helicase associated domain (HA2), winged-helix
HA2_C	PF21010	502 - 561	6.9e-15	Helicase associated domain (HA2), ratchet-like
OB_NTP_bind	PF07717	623 - 695	7.7e-21	Oligonucleotide/oligosaccharide-binding (OB)-fold

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

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Align Labels (Cladogram)

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Orthologous Genes (Group: OG0015858)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT5G14900
fragaria_vesca	FvH4_2g20600 FvH4_2g20610
malus_domestica	MD13G1065100.v1.1
pyrus_communis	pycom13g05740
rosa_chinensis	RchiOBHm_Chr6g0286781
rosa_laevigata	RLG00000012542
rosa_multiflora	Rmu_sc0007546.1_g000017
rosa_roxburghii	Rroxscaffold_7G00182020
rosa_rugosa	Rorug06G0182900
rosa_samantha	Rh6AG295500 Rh6BG299600 Rh6CG299400 Rh6DG291300
rosa_wichuraiana	Rw6G025440

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AanI	TTATAA	1 cut(s)	1143
AasI	GACNNNNNNGTC	1 cut(s)	59
Acc16I	TGCGCA	1 cut(s)	1799
Acc36I	ACCTGC	1 cut(s)	1891
AccB1I	GGYRCC	1 cut(s)	257
AccB7I	CCANNNNNTGG	1 cut(s)	95
AccII	CGCG	1 cut(s)	389
AciI	CCGC	3 cut(s)	430, 507, 1923
AclWI	GGATC	5 cut(s)	184, 560, 1382, 1395, 1992
AcoI	YGGCCR	1 cut(s)	624
AcsI	RAATTY	3 cut(s)	1035, 1493, 1975
AcuI	CTGAAG	1 cut(s)	701
AfiI	CCNNNNNNNGG	6 cut(s)	95, 409, 493, 685, 1476, 1612
AflII	CTTAAG	1 cut(s)	1840
AgsI	TTSAA	5 cut(s)	523, 664, 996, 1625, 1765
AhlI	ACTAGT	1 cut(s)	128
AjiI	CACGTC	1 cut(s)	1202
AjnI	CCWGG	3 cut(s)	760, 865, 1727
AluBI	AGCT	9 cut(s)	40, 332, 608, 674, 685, 1646, 1792, 1825, 1907
AluI	AGCT	9 cut(s)	40, 332, 608, 674, 685, 1646, 1792, 1825, 1907
Alw21I	GWGCWC	3 cut(s)	503, 745, 1201
Alw26I	GTCTC	4 cut(s)	67, 1182, 1286, 1397
Alw44I	GTGCAC	3 cut(s)	499, 741, 1197
AlwI	GGATC	5 cut(s)	184, 560, 1382, 1395, 1992
AlwNI	CAGNNNCTG	2 cut(s)	1792, 1933
AoxI	GGCC	4 cut(s)	164, 624, 711, 1607
ApaLI	GTGCAC	3 cut(s)	499, 741, 1197
ApeKI	GCWGC	8 cut(s)	366, 413, 824, 1769, 1786, 1789, 1845, 1920
ApoI	RAATTY	3 cut(s)	1035, 1493, 1975
AseI	ATTAAT	2 cut(s)	927, 939
Asp700I	GAANNNNTTC	1 cut(s)	1268
AspA2I	CCTAGG	1 cut(s)	1610
AspLEI	GCGC	2 cut(s)	1156, 1800
AspS9I	GGNCC	2 cut(s)	968, 1720
AsuC2I	CCSGG	2 cut(s)	1119, 1236
AsuHPI	GGTGA	4 cut(s)	293, 1664, 2062, 2078
AvaII	GGWCC	2 cut(s)	968, 1720
AvrII	CCTAGG	1 cut(s)	1610
BaeGI	GKGCMC	5 cut(s)	260, 503, 745, 1201, 1593
BalI	TGGCCA	1 cut(s)	626
BamHI	GGATCC	1 cut(s)	1387
BanI	GGYRCC	1 cut(s)	257
BanII	GRGCYC	1 cut(s)	1447
BauI	CACGAG	1 cut(s)	1149
BbsI	GAAGAC	1 cut(s)	518
Bbv12I	GWGCWC	3 cut(s)	503, 745, 1201
BbvCI	CCTCAGC	1 cut(s)	441
BbvI	GCAGC	8 cut(s)	378, 400, 836, 1756, 1798, 1801, 1857, 1932
BccI	CCATC	6 cut(s)	442, 530, 1088, 1405, 1451, 1732
BceAI	ACGGC	1 cut(s)	417
BcgI	CGANNNNNNTGC	2 cut(s)	472, 506
BciT130I	CCWGG	3 cut(s)	762, 867, 1729
BclI	TGATCA	1 cut(s)	1030
BcnI	CCSGG	2 cut(s)	1119, 1236
BcoDI	GTCTC	4 cut(s)	67, 1182, 1286, 1397
BcuI	ACTAGT	1 cut(s)	128
BfaI	CTAG	8 cut(s)	6, 129, 1352, 1421, 1502, 1611, 1938, 2075
BfmI	CTRYAG	1 cut(s)	1770
BfrI	CTTAAG	1 cut(s)	1840
BfuAI	ACCTGC	1 cut(s)	1891
BisI	GCNGC	8 cut(s)	367, 414, 825, 1770, 1787, 1790, 1846, 1921
BlnI	CCTAGG	1 cut(s)	1610
BlsI	GCNGC	8 cut(s)	368, 415, 826, 1771, 1788, 1791, 1847, 1922
BmcAI	AGTACT	3 cut(s)	527, 1335, 1834
Bme1390I	CCNGG	5 cut(s)	762, 867, 1119, 1236, 1729
Bme18I	GGWCC	2 cut(s)	968, 1720
BmgBI	CACGTC	1 cut(s)	1202
BmgT120I	GGNCC	2 cut(s)	968, 1720
BmiI	GGNNCC	5 cut(s)	259, 864, 1389, 1444, 1722
BmrFI	CCNGG	5 cut(s)	762, 867, 1119, 1236, 1729
BmsI	GCATC	8 cut(s)	492, 901, 972, 1405, 1512, 1569, 1785, 1975
BpiI	GAAGAC	1 cut(s)	518
BplI	GAGNNNNNCTC	4 cut(s)	688, 720, 1659, 1691
BpmI	CTGGAG	2 cut(s)	888, 912
Bpu10I	CCTNAGC	1 cut(s)	441
BpuEI	CTTGAG	6 cut(s)	215, 236, 635, 728, 1304, 1805
BpuMI	CCSGG	2 cut(s)	1119, 1236
BsaAI	YACGTR	2 cut(s)	20, 817
BsaBI	GATNNNNATC	1 cut(s)	383
BsaI	GGTCTC	1 cut(s)	67
BsaJI	CCNNGG	3 cut(s)	1117, 1470, 1610
BsaXI	ACNNNNNCTCC	6 cut(s)	456, 486, 773, 803, 1535, 1565
Bsc4I	CCNNNNNNNGG	6 cut(s)	95, 409, 493, 685, 1476, 1612
Bse118I	RCCGGY	1 cut(s)	287
Bse1I	ACTGG	3 cut(s)	163, 895, 955
Bse3DI	GCAATG	3 cut(s)	423, 564, 1068
Bse8I	GATNNNNATC	1 cut(s)	383
BseBI	CCWGG	3 cut(s)	762, 867, 1729
BseDI	CCNNGG	3 cut(s)	1117, 1470, 1610
BseGI	GGATG	7 cut(s)	65, 457, 483, 1420, 1621, 1724, 1966
BseJI	GATNNNNATC	1 cut(s)	383
BseLI	CCNNNNNNNGG	6 cut(s)	95, 409, 493, 685, 1476, 1612
BseMI	GCAATG	3 cut(s)	423, 564, 1068
BseNI	ACTGG	3 cut(s)	163, 895, 955
BseRI	GAGGAG	5 cut(s)	94, 117, 217, 458, 509
BseSI	GKGCMC	5 cut(s)	260, 503, 745, 1201, 1593
BseXI	GCAGC	8 cut(s)	378, 400, 836, 1756, 1798, 1801, 1857, 1932
Bsh1236I	CGCG	1 cut(s)	389
BshFI	GGCC	4 cut(s)	166, 626, 713, 1609
BshNI	GGYRCC	1 cut(s)	257
BsiHKAI	GWGCWC	3 cut(s)	503, 745, 1201
BsiSI	CCGG	5 cut(s)	288, 1119, 1221, 1235, 1606
BslFI	GGGAC	1 cut(s)	412
BslI	CCNNNNNNNGG	6 cut(s)	95, 409, 493, 685, 1476, 1612
BsmAI	GTCTC	4 cut(s)	67, 1182, 1286, 1397
BsmFI	GGGAC	1 cut(s)	412
BsnI	GGCC	4 cut(s)	166, 626, 713, 1609
Bso31I	GGTCTC	1 cut(s)	67
Bsp1286I	GDGCHC	6 cut(s)	260, 503, 745, 1201, 1447, 1593
Bsp1407I	TGTACA	1 cut(s)	789
Bsp19I	CCATGG	1 cut(s)	1470
BspACI	CCGC	3 cut(s)	430, 507, 1923
BspANI	GGCC	4 cut(s)	166, 626, 713, 1609
BspFNI	CGCG	1 cut(s)	389
BspHI	TCATGA	1 cut(s)	610
BspLI	GGNNCC	5 cut(s)	259, 864, 1389, 1444, 1722
BspMAI	CTGCAG	1 cut(s)	1774
BspMI	ACCTGC	1 cut(s)	1891
BspPI	GGATC	5 cut(s)	184, 560, 1382, 1395, 1992
BspT107I	GGYRCC	1 cut(s)	257
BspTI	CTTAAG	1 cut(s)	1840
BspTNI	GGTCTC	1 cut(s)	67
BsrDI	GCAATG	3 cut(s)	423, 564, 1068
BsrFI	RCCGGY	1 cut(s)	287
BsrGI	TGTACA	1 cut(s)	789
BsrI	ACTGG	3 cut(s)	163, 895, 955
BssAI	RCCGGY	1 cut(s)	287
BssECI	CCNNGG	3 cut(s)	1117, 1470, 1610
BssSI	CACGAG	1 cut(s)	1149
BssT1I	CCWWGG	2 cut(s)	1470, 1610
Bst2BI	CACGAG	1 cut(s)	1149
Bst2UI	CCWGG	3 cut(s)	762, 867, 1729
Bst4CI	ACNGT	4 cut(s)	838, 1333, 1929, 2050
Bst6I	CTCTTC	3 cut(s)	26, 194, 462
BstAFI	CTTAAG	1 cut(s)	1840
BstAPI	GCANNNNNTGC	1 cut(s)	1795
BstAUI	TGTACA	1 cut(s)	789
BstBAI	YACGTR	2 cut(s)	20, 817
BstC8I	GCNNGC	4 cut(s)	767, 916, 1218, 1884
BstDSI	CCRYGG	1 cut(s)	1470
BstEII	GGTNACC	1 cut(s)	2050
BstF5I	GGATG	7 cut(s)	65, 457, 483, 1420, 1621, 1724, 1966
BstFNI	CGCG	1 cut(s)	389
BstHHI	GCGC	2 cut(s)	1156, 1800
BstMAI	GTCTC	4 cut(s)	67, 1182, 1286, 1397
BstMWI	GCNNNNNNNGC	6 cut(s)	1588, 1637, 1652, 1766, 1795, 1883
BstNI	CCWGG	3 cut(s)	762, 867, 1729
BstNSI	RCATGY	2 cut(s)	918, 1160
BstPI	GGTNACC	1 cut(s)	2050
BstSCI	CCNGG	5 cut(s)	760, 865, 1117, 1234, 1727
BstSFI	CTRYAG	1 cut(s)	1770
BstSLI	GKGCMC	5 cut(s)	260, 503, 745, 1201, 1593
BstSNI	TACGTA	2 cut(s)	20, 817
BstUI	CGCG	1 cut(s)	389
BstV1I	GCAGC	8 cut(s)	378, 400, 836, 1756, 1798, 1801, 1857, 1932
BstV2I	GAAGAC	1 cut(s)	518
BstX2I	RGATCY	3 cut(s)	189, 565, 1387
BstXI	CCANNNNNNTGG	1 cut(s)	2063
BstYI	RGATCY	3 cut(s)	189, 565, 1387
BsuRI	GGCC	4 cut(s)	166, 626, 713, 1609
BtgI	CCRYGG	1 cut(s)	1470
BtgZI	GCGATG	1 cut(s)	1592
BtrI	CACGTC	1 cut(s)	1202
BtsCI	GGATG	7 cut(s)	65, 457, 483, 1420, 1621, 1724, 1966
BtsI	GCAGTG	1 cut(s)	298
BtsIMutI	CAGTG	4 cut(s)	298, 429, 627, 962
BveI	ACCTGC	1 cut(s)	1891
Cac8I	GCNNGC	4 cut(s)	767, 916, 1218, 1884
CaiI	CAGNNNCTG	2 cut(s)	1792, 1933
CciI	TCATGA	1 cut(s)	610
CfoI	GCGC	2 cut(s)	1156, 1800
Cfr10I	RCCGGY	1 cut(s)	287
Cfr13I	GGNCC	2 cut(s)	968, 1720
CviAII	CATG	7 cut(s)	611, 915, 1157, 1342, 1384, 1471, 1697
DrdI	GACNNNNNNGTC	1 cut(s)	59
DseDI	GACNNNNNNGTC	1 cut(s)	59
EaeI	YGGCCR	1 cut(s)	624
Eam1104I	CTCTTC	3 cut(s)	26, 194, 462
EarI	CTCTTC	3 cut(s)	26, 194, 462
Eco105I	TACGTA	2 cut(s)	20, 817
Eco130I	CCWWGG	2 cut(s)	1470, 1610
Eco147I	AGGCCT	1 cut(s)	166
Eco24I	GRGCYC	1 cut(s)	1447
Eco31I	GGTCTC	1 cut(s)	67
Eco47I	GGWCC	2 cut(s)	968, 1720
Eco57I	CTGAAG	1 cut(s)	701
Eco91I	GGTNACC	1 cut(s)	2050
EcoO109I	RGGNCCY	1 cut(s)	1720
EcoO65I	GGTNACC	1 cut(s)	2050
EcoRI	GAATTC	1 cut(s)	1493
EcoRII	CCWGG	3 cut(s)	760, 865, 1727
EcoT14I	CCWWGG	2 cut(s)	1470, 1610
EcoT22I	ATGCAT	1 cut(s)	916
EcoT38I	GRGCYC	1 cut(s)	1447
ErhI	CCWWGG	2 cut(s)	1470, 1610
FaeI	CATG	7 cut(s)	614, 918, 1160, 1345, 1387, 1474, 1700
FalI	AAGNNNNNCTT	2 cut(s)	29, 61
FaqI	GGGAC	1 cut(s)	412
FatI	CATG	7 cut(s)	610, 914, 1156, 1341, 1383, 1470, 1696
FauI	CCCGC	2 cut(s)	437, 500
FbaI	TGATCA	1 cut(s)	1030
Fnu4HI	GCNGC	8 cut(s)	367, 414, 825, 1770, 1787, 1790, 1846, 1921
FokI	GGATG	7 cut(s)	52, 464, 470, 1427, 1628, 1711, 1953
FriOI	GRGCYC	1 cut(s)	1447
Fsp4HI	GCNGC	8 cut(s)	367, 414, 825, 1770, 1787, 1790, 1846, 1921
FspAI	RTGCGCAY	1 cut(s)	1799
FspBI	CTAG	8 cut(s)	6, 129, 1352, 1421, 1502, 1611, 1938, 2075
FspI	TGCGCA	1 cut(s)	1799
GlaI	GCGC	2 cut(s)	1155, 1799
GluI	GCNGC	8 cut(s)	367, 414, 825, 1770, 1787, 1790, 1846, 1921
GsuI	CTGGAG	2 cut(s)	888, 912
HaeIII	GGCC	4 cut(s)	166, 626, 713, 1609
HapII	CCGG	5 cut(s)	288, 1119, 1221, 1235, 1606
HhaI	GCGC	2 cut(s)	1156, 1800
Hin1II	CATG	7 cut(s)	614, 918, 1160, 1345, 1387, 1474, 1700
Hin6I	GCGC	2 cut(s)	1154, 1798
HinP1I	GCGC	2 cut(s)	1154, 1798
HincII	GTYRAC	2 cut(s)	1062, 1114
HindII	GTYRAC	2 cut(s)	1062, 1114
HinfI	GANTC	6 cut(s)	71, 310, 646, 847, 1163, 1369
HpaII	CCGG	5 cut(s)	288, 1119, 1221, 1235, 1606
HphI	GGTGA	4 cut(s)	293, 1664, 2062, 2078
Hpy166II	GTNNAC	8 cut(s)	156, 393, 501, 743, 792, 1062, 1114, 1199
Hpy8I	GTNNAC	8 cut(s)	156, 393, 501, 743, 792, 1062, 1114, 1199
Hpy99I	CGWCG	2 cut(s)	408, 1637
HpyAV	CCTTC	2 cut(s)	156, 1759
HpyCH4III	ACNGT	4 cut(s)	838, 1333, 1929, 2050
HpyCH4IV	ACGT	3 cut(s)	19, 816, 1201
HpyF10VI	GCNNNNNNNGC	6 cut(s)	1588, 1637, 1652, 1766, 1795, 1883
HpySE526I	ACGT	3 cut(s)	19, 816, 1201
Hsp92II	CATG	7 cut(s)	614, 918, 1160, 1345, 1387, 1474, 1700
HspAI	GCGC	2 cut(s)	1154, 1798
Ksp22I	TGATCA	1 cut(s)	1030
LmnI	GCTCC	6 cut(s)	81, 104, 868, 1402, 1442, 1556
Lsp1109I	GCAGC	8 cut(s)	378, 400, 836, 1756, 1798, 1801, 1857, 1932
LweI	GCATC	8 cut(s)	492, 901, 972, 1405, 1512, 1569, 1785, 1975
MaeI	CTAG	8 cut(s)	6, 129, 1352, 1421, 1502, 1611, 1938, 2075
MaeII	ACGT	3 cut(s)	19, 816, 1201
MaeIII	GTNAC	3 cut(s)	476, 1460, 2050
MboII	GAAGA	9 cut(s)	43, 211, 388, 479, 523, 626, 920, 1236, 1466
MflI	RGATCY	3 cut(s)	189, 565, 1387
MhlI	GDGCHC	6 cut(s)	260, 503, 745, 1201, 1447, 1593
MlsI	TGGCCA	1 cut(s)	626
MluNI	TGGCCA	1 cut(s)	626
MlyI	GAGTC	3 cut(s)	65, 1172, 1378
MmeI	TCCRAC	1 cut(s)	93
Mox20I	TGGCCA	1 cut(s)	626
Mph1103I	ATGCAT	1 cut(s)	916
MroXI	GAANNNNTTC	1 cut(s)	1268
MscI	TGGCCA	1 cut(s)	626
MseI	TTAA	7 cut(s)	530, 548, 735, 927, 939, 1815, 1841
MslI	CAYNNNNRTG	1 cut(s)	1729
Msp20I	TGGCCA	1 cut(s)	626
MspA1I	CMGCKG	3 cut(s)	507, 1792, 1923
MspCI	CTTAAG	1 cut(s)	1840
MspI	CCGG	5 cut(s)	288, 1119, 1221, 1235, 1606
MspR9I	CCNGG	5 cut(s)	762, 867, 1119, 1236, 1729
MvaI	CCWGG	3 cut(s)	762, 867, 1729
MvnI	CGCG	1 cut(s)	389
MwoI	GCNNNNNNNGC	6 cut(s)	1588, 1637, 1652, 1766, 1795, 1883
NciI	CCSGG	2 cut(s)	1119, 1236
NcoI	CCATGG	1 cut(s)	1470
NlaIII	CATG	7 cut(s)	614, 918, 1160, 1345, 1387, 1474, 1700
NlaIV	GGNNCC	5 cut(s)	259, 864, 1389, 1444, 1722
NmeAIII	GCCGAG	1 cut(s)	364
NmuCI	GTSAC	1 cut(s)	2050
NsbI	TGCGCA	1 cut(s)	1799
NsiI	ATGCAT	1 cut(s)	916
NspI	RCATGY	2 cut(s)	918, 1160
PaeI	GCATGC	1 cut(s)	918
PagI	TCATGA	1 cut(s)	610
PceI	AGGCCT	1 cut(s)	166
PdmI	GAANNNNTTC	1 cut(s)	1268
PfeI	GAWTC	3 cut(s)	310, 646, 847
PflMI	CCANNNNNTGG	1 cut(s)	95
PfoI	TCCNGGA	1 cut(s)	865
PkrI	GCNGC	8 cut(s)	368, 415, 826, 1771, 1788, 1791, 1847, 1922
PleI	GAGTC	3 cut(s)	65, 1171, 1377
PpsI	GAGTC	3 cut(s)	65, 1171, 1377
Ppu21I	YACGTR	2 cut(s)	20, 817
PpuMI	RGGWCCY	1 cut(s)	1720
PshBI	ATTAAT	2 cut(s)	927, 939
PsiI	TTATAA	1 cut(s)	1143
Psp5II	RGGWCCY	1 cut(s)	1720
Psp6I	CCWGG	3 cut(s)	760, 865, 1727
PspEI	GGTNACC	1 cut(s)	2050
PspGI	CCWGG	3 cut(s)	760, 865, 1727
PspN4I	GGNNCC	5 cut(s)	259, 864, 1389, 1444, 1722
PspPI	GGNCC	2 cut(s)	968, 1720
PspPPI	RGGWCCY	1 cut(s)	1720
PstI	CTGCAG	1 cut(s)	1774
PstNI	CAGNNNCTG	2 cut(s)	1792, 1933
PsuI	RGATCY	3 cut(s)	189, 565, 1387
PvuII	CAGCTG	1 cut(s)	1792
RseI	CAYNNNNRTG	1 cut(s)	1729
SaqAI	TTAA	7 cut(s)	530, 548, 735, 927, 939, 1815, 1841
SatI	GCNGC	8 cut(s)	367, 414, 825, 1770, 1787, 1790, 1846, 1921
Sau96I	GGNCC	2 cut(s)	968, 1720
ScaI	AGTACT	3 cut(s)	527, 1335, 1834
SchI	GAGTC	3 cut(s)	65, 1172, 1378
ScrFI	CCNGG	5 cut(s)	762, 867, 1119, 1236, 1729
SduI	GDGCHC	6 cut(s)	260, 503, 745, 1201, 1447, 1593
SfaNI	GCATC	8 cut(s)	492, 901, 972, 1405, 1512, 1569, 1785, 1975
SfcI	CTRYAG	1 cut(s)	1770
SinI	GGWCC	2 cut(s)	968, 1720
SmiMI	CAYNNNNRTG	1 cut(s)	1729
SmlI	CTYRAG	7 cut(s)	194, 251, 650, 707, 1283, 1820, 1840
SmoI	CTYRAG	7 cut(s)	194, 251, 650, 707, 1283, 1820, 1840
SnaBI	TACGTA	2 cut(s)	20, 817
SpeI	ACTAGT	1 cut(s)	128
SphI	GCATGC	1 cut(s)	918
SseBI	AGGCCT	1 cut(s)	166
SsiI	CCGC	3 cut(s)	430, 507, 1923
SspMI	CTAG	8 cut(s)	6, 129, 1352, 1421, 1502, 1611, 1938, 2075
StuI	AGGCCT	1 cut(s)	166
StyD4I	CCNGG	5 cut(s)	760, 865, 1117, 1234, 1727
StyI	CCWWGG	2 cut(s)	1470, 1610
TaaI	ACNGT	4 cut(s)	838, 1333, 1929, 2050
TaiI	ACGT	3 cut(s)	22, 819, 1204
TaqI	TCGA	6 cut(s)	51, 123, 406, 492, 724, 2079
TaqII	GACCGA	1 cut(s)	49
TatI	WGTACW	6 cut(s)	392, 525, 789, 1333, 1832, 1957
TfiI	GAWTC	3 cut(s)	310, 646, 847
Tru1I	TTAA	7 cut(s)	530, 548, 735, 927, 939, 1815, 1841
Tru9I	TTAA	7 cut(s)	530, 548, 735, 927, 939, 1815, 1841
TscAI	CASTG	4 cut(s)	298, 429, 634, 962
TseFI	GTSAC	1 cut(s)	2050
TseI	GCWGC	8 cut(s)	366, 413, 824, 1769, 1786, 1789, 1845, 1920
Tsp45I	GTSAC	1 cut(s)	2050
TspDTI	ATGAA	4 cut(s)	627, 1358, 1372, 1467
TspGWI	ACGGA	3 cut(s)	476, 1576, 1680
TspRI	CASTG	4 cut(s)	298, 429, 634, 962
Van91I	CCANNNNNTGG	1 cut(s)	95
Vha464I	CTTAAG	1 cut(s)	1840
VneI	GTGCAC	3 cut(s)	499, 741, 1197
VpaK11BI	GGWCC	2 cut(s)	968, 1720
VspI	ATTAAT	2 cut(s)	927, 939
XapI	RAATTY	3 cut(s)	1035, 1493, 1975
XceI	RCATGY	2 cut(s)	918, 1160
XcmI	CCANNNNNNNNNTGG	1 cut(s)	962
XmaJI	CCTAGG	1 cut(s)	1610
XmnI	GAANNNNTTC	1 cut(s)	1268
XspI	CTAG	8 cut(s)	6, 129, 1352, 1421, 1502, 1611, 1938, 2075
ZrmI	AGTACT	3 cut(s)	527, 1335, 1834
Zsp2I	ATGCAT	1 cut(s)	916

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.