pycom13g05740

Pre-mRNA-splicing factor ATP-dependent RNA

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr13
OG0015858
Physical Location & Seq
Forward (+)
3791187 .. 3792008
822 bp
Loading structure...
UTR
Exon/CDS
Intron
pycom13g05740.1

Sequence Viewer

Length: 822 bp
ATGGGAGATCTTGAAGCTGCCGGAGATAAACTCGTTTTCCTCCCCAAAGAGGACGAGATAGAAAGCGTGTGCTGCAAAATTAACCAAGCAATTGCAATTGCTTCTGCTCGGTTTCAGGTATCTGCACCTGTCAAAATCGTGCCTCTGTATTCGGAAACTCTTGGTCTTGCGCCATCGGATCAAGAACAAGTGTTCGATCAAATTCCTGGGAGGAAGATTGTCGTCTCAACAGACATTGCAGAAACCTCTCTAACCGTACCTGGAATTGTTTATGTTATTGACACGGGATTGGTTGCACATAAAGTATATAGCCCACAGTGCCCCCCTGTCCCTGTTGAATCTTTGTTGGTGTCTCCAATTTCTAGGCCAAGTGCCTCTCTGAGGCATGAACGTGCCGGGGGAACCCGGCCCGGGAAATGTTTCAGACTTTACACTGAGAATAGTCTCCGAAACCATCTTCAAAGACGCACACAGACAGAAATTTGTAGGTCCAAATTTGCTTCGGCAGCTCTTACTTTGAGGAAAATAGTTCTCATGGATCTGATGCTAGTTGATTTTGTGGAGACTCCTCATCCCGAGCAAATGATGGAAGCGATAGAGATCTTGAAGCGTCTGGGAGCAATGGATGACGAATACCGCTGGACCGAGCTGGGGGAGATTATGAGCGAGTTCCCCTTAGATCCTCAGCTATCGAAGATGCTTATTGTTTGCCCTAAACTCAACTGCTCACATAAGATTTTGTCCATTTGTGCCATGCTTTCAGTACCGTTGAGTCCCCTTGTTCAGCCTCGGGGGAGGCTCAAAAATCGGCTGCGCTGCTGA

Protein Analysis

274

Amino Acids

30.35

Weight (kDa)

7.55

Isoelectric Point (pI)

50.75

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
HA2_C PF21010 227 - 266 1.3e-07 Helicase associated domain (HA2), ratchet-like
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
iTOL Interactive Tree Of Life · Most popular EvolView Flexible annotations MEGA Desktop phylogenetics suite FigTree Desktop tree editor

Orthologous Genes (Group: OG0015858)

Species Orthologous Gene IDs
arabidopsis_thaliana AT5G14900
fragaria_vesca FvH4_2g20600 FvH4_2g20610
malus_domestica MD13G1065100.v1.1
pyrus_communis pycom13g05740
rosa_chinensis RchiOBHm_Chr6g0286781
rosa_laevigata RLG00000012542
rosa_multiflora Rmu_sc0007546.1_g000017
rosa_roxburghii Rroxscaffold_7G00182020
rosa_rugosa Rorug06G0182900
rosa_samantha Rh6AG295500 Rh6BG299600 Rh6CG299400 Rh6DG291300
rosa_wichuraiana Rw6G025440

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AciI CCGC 1 cut(s) 637
AclWI GGATC 3 cut(s) 186, 546, 674
AcsI RAATTY 3 cut(s) 201, 480, 494
AfaI GTAC 2 cut(s) 258, 765
AfiI CCNNNNNNNGG 4 cut(s) 49, 381, 411, 651
AgsI TTSAA 4 cut(s) 14, 338, 461, 607
AjnI CCWGG 2 cut(s) 205, 259
AluBI AGCT 4 cut(s) 17, 509, 649, 688
AluI AGCT 4 cut(s) 17, 509, 649, 688
Alw26I GTCTC 4 cut(s) 229, 357, 449, 557
AlwI GGATC 3 cut(s) 186, 546, 674
Ama87I CYCGRG 3 cut(s) 410, 575, 789
AoxI GGCC 2 cut(s) 365, 407
ApeKI GCWGC 5 cut(s) 17, 72, 506, 811, 816
ApoI RAATTY 3 cut(s) 201, 480, 494
Asp700I GAANNNNTTC 1 cut(s) 419
AspLEI GCGC 2 cut(s) 172, 816
AspS9I GGNCC 3 cut(s) 408, 489, 642
AsuC2I CCSGG 4 cut(s) 397, 406, 411, 412
AvaI CYCGRG 3 cut(s) 410, 575, 789
AvaII GGWCC 2 cut(s) 489, 642
BaeGI GKGCMC 1 cut(s) 323
BbvCI CCTCAGC 1 cut(s) 684
BbvI GCAGC 5 cut(s) 4, 59, 518, 798, 803
BccI CCATC 3 cut(s) 181, 462, 580
BciT130I CCWGG 2 cut(s) 207, 261
BcnI CCSGG 4 cut(s) 397, 406, 411, 412
BcoDI GTCTC 4 cut(s) 229, 357, 449, 557
BfaI CTAG 2 cut(s) 363, 548
BglII AGATCT 2 cut(s) 7, 600
BisI GCNGC 5 cut(s) 18, 73, 507, 812, 817
BlsI GCNGC 5 cut(s) 19, 74, 508, 813, 818
Bme1390I CCNGG 6 cut(s) 207, 261, 397, 406, 411, 412
Bme18I GGWCC 2 cut(s) 489, 642
BmeT110I CYCGRG 3 cut(s) 410, 575, 789
BmgT120I GGNCC 3 cut(s) 408, 489, 642
BmiI GGNNCC 1 cut(s) 403
BmrFI CCNGG 6 cut(s) 207, 261, 397, 406, 411, 412
BmsI GCATC 2 cut(s) 534, 687
BplI GAGNNNNNCTC 2 cut(s) 15, 47
Bpu10I CCTNAGC 1 cut(s) 684
BpuMI CCSGG 4 cut(s) 397, 406, 411, 412
BsaBI GATNNNNATC 1 cut(s) 599
BsaJI CCNNGG 4 cut(s) 206, 396, 410, 788
Bsc4I CCNNNNNNNGG 4 cut(s) 49, 381, 411, 651
Bse3DI GCAATG 2 cut(s) 234, 627
Bse8I GATNNNNATC 1 cut(s) 599
BseBI CCWGG 2 cut(s) 207, 261
BseDI CCNNGG 4 cut(s) 206, 396, 410, 788
BseGI GGATG 2 cut(s) 571, 631
BseJI GATNNNNATC 1 cut(s) 599
BseLI CCNNNNNNNGG 4 cut(s) 49, 381, 411, 651
BseMI GCAATG 2 cut(s) 234, 627
BseMII CTCAG 3 cut(s) 371, 426, 698
BseRI GAGGAG 1 cut(s) 558
BseSI GKGCMC 1 cut(s) 323
BseXI GCAGC 5 cut(s) 4, 59, 518, 798, 803
BseYI CCCAGC 1 cut(s) 649
BsgI GTGCAG 1 cut(s) 108
BshFI GGCC 2 cut(s) 367, 409
BsiHKCI CYCGRG 3 cut(s) 410, 575, 789
BsiSI CCGG 4 cut(s) 21, 396, 406, 411
BslFI GGGAC 2 cut(s) 314, 759
BslI CCNNNNNNNGG 4 cut(s) 49, 381, 411, 651
BsmAI GTCTC 4 cut(s) 229, 357, 449, 557
BsmBI CGTCTC 1 cut(s) 229
BsmFI GGGAC 2 cut(s) 314, 759
BsnI GGCC 2 cut(s) 367, 409
BsoBI CYCGRG 3 cut(s) 410, 575, 789
Bsp1286I GDGCHC 1 cut(s) 323
Bsp143I GATC 6 cut(s) 7, 178, 196, 538, 600, 679
BspACI CCGC 1 cut(s) 637
BspANI GGCC 2 cut(s) 367, 409
BspCNI CTCAG 3 cut(s) 372, 427, 697
BspLI GGNNCC 1 cut(s) 403
BspPI GGATC 3 cut(s) 186, 546, 674
BsrDI GCAATG 2 cut(s) 234, 627
BssECI CCNNGG 4 cut(s) 206, 396, 410, 788
BssMI GATC 6 cut(s) 7, 178, 196, 538, 600, 679
Bst2UI CCWGG 2 cut(s) 207, 261
Bst4CI ACNGT 3 cut(s) 256, 318, 768
BstDEI CTNAG 4 cut(s) 380, 435, 676, 684
BstENI CCTNNNNNAGG 1 cut(s) 379
BstF5I GGATG 2 cut(s) 571, 631
BstHHI GCGC 2 cut(s) 172, 816
BstKTI GATC 6 cut(s) 10, 181, 199, 541, 603, 682
BstMAI GTCTC 4 cut(s) 229, 357, 449, 557
BstMBI GATC 6 cut(s) 7, 178, 196, 538, 600, 679
BstMWI GCNNNNNNNGC 3 cut(s) 72, 318, 506
BstNI CCWGG 2 cut(s) 207, 261
BstSCI CCNGG 6 cut(s) 205, 259, 395, 404, 409, 410
BstSLI GKGCMC 1 cut(s) 323
BstV1I GCAGC 5 cut(s) 4, 59, 518, 798, 803
BstX2I RGATCY 4 cut(s) 7, 538, 600, 679
BstYI RGATCY 4 cut(s) 7, 538, 600, 679
BsuRI GGCC 2 cut(s) 367, 409
BtsCI GGATG 2 cut(s) 571, 631
BtsIMutI CAGTG 2 cut(s) 323, 432
CfoI GCGC 2 cut(s) 172, 816
Cfr13I GGNCC 3 cut(s) 408, 489, 642
Cfr9I CCCGGG 1 cut(s) 410
CseI GACGC 2 cut(s) 474, 599
Csp6I GTAC 2 cut(s) 257, 764
CviAII CATG 3 cut(s) 386, 535, 754
CviQI GTAC 2 cut(s) 257, 764
DdeI CTNAG 4 cut(s) 380, 435, 676, 684
DpnI GATC 6 cut(s) 9, 180, 198, 540, 602, 681
DpnII GATC 6 cut(s) 7, 178, 196, 538, 600, 679
Eco47I GGWCC 2 cut(s) 489, 642
Eco88I CYCGRG 3 cut(s) 410, 575, 789
EcoNI CCTNNNNNAGG 1 cut(s) 379
EcoRII CCWGG 2 cut(s) 205, 259
Esp3I CGTCTC 1 cut(s) 229
FaeI CATG 3 cut(s) 389, 538, 757
FaiI YATR 9 cut(s) 273, 300, 307, 309, 387, 536, 662, 732, 755
FaqI GGGAC 2 cut(s) 314, 759
FatI CATG 3 cut(s) 385, 534, 753
Fnu4HI GCNGC 5 cut(s) 18, 73, 507, 812, 817
FokI GGATG 2 cut(s) 558, 638
Fsp4HI GCNGC 5 cut(s) 18, 73, 507, 812, 817
FspBI CTAG 2 cut(s) 363, 548
GlaI GCGC 2 cut(s) 171, 815
GluI GCNGC 5 cut(s) 18, 73, 507, 812, 817
GsaI CCCAGC 1 cut(s) 653
HaeIII GGCC 2 cut(s) 367, 409
HapII CCGG 4 cut(s) 21, 396, 406, 411
HgaI GACGC 2 cut(s) 474, 599
HhaI GCGC 2 cut(s) 172, 816
Hin1II CATG 3 cut(s) 389, 538, 757
Hin6I GCGC 2 cut(s) 170, 814
HinP1I GCGC 2 cut(s) 170, 814
HinfI GANTC 3 cut(s) 338, 565, 772
HpaII CCGG 4 cut(s) 21, 396, 406, 411
Hpy188I TCNGA 6 cut(s) 154, 178, 381, 425, 449, 543
Hpy188III TCNNGA 4 cut(s) 11, 182, 575, 604
HpyCH4III ACNGT 3 cut(s) 256, 318, 768
HpyCH4IV ACGT 1 cut(s) 391
HpyCH4V TGCA 5 cut(s) 75, 95, 125, 239, 296
HpyF10VI GCNNNNNNNGC 3 cut(s) 72, 318, 506
HpyF3I CTNAG 4 cut(s) 380, 435, 676, 684
HpySE526I ACGT 1 cut(s) 391
Hsp92II CATG 3 cut(s) 389, 538, 757
HspAI GCGC 2 cut(s) 170, 814
Kzo9I GATC 6 cut(s) 7, 178, 196, 538, 600, 679
LmnI GCTCC 1 cut(s) 617
Lsp1109I GCAGC 5 cut(s) 4, 59, 518, 798, 803
LweI GCATC 2 cut(s) 534, 687
MaeI CTAG 2 cut(s) 363, 548
MaeII ACGT 1 cut(s) 391
MalI GATC 6 cut(s) 9, 180, 198, 540, 602, 681
MboI GATC 6 cut(s) 7, 178, 196, 538, 600, 679
MboII GAAGA 3 cut(s) 226, 449, 706
MfeI CAATTG 2 cut(s) 90, 96
MflI RGATCY 4 cut(s) 7, 538, 600, 679
MhlI GDGCHC 1 cut(s) 323
MluCI AATT 8 cut(s) 78, 90, 96, 201, 264, 357, 480, 494
MlyI GAGTC 2 cut(s) 559, 781
MroXI GAANNNNTTC 1 cut(s) 419
MseI TTAA 1 cut(s) 81
MslI CAYNNNNRTG 1 cut(s) 390
MspA1I CMGCKG 1 cut(s) 639
MspI CCGG 4 cut(s) 21, 396, 406, 411
MspR9I CCNGG 6 cut(s) 207, 261, 397, 406, 411, 412
MunI CAATTG 2 cut(s) 90, 96
MvaI CCWGG 2 cut(s) 207, 261
MwoI GCNNNNNNNGC 3 cut(s) 72, 318, 506
NciI CCSGG 4 cut(s) 397, 406, 411, 412
NdeII GATC 6 cut(s) 7, 178, 196, 538, 600, 679
NlaIII CATG 3 cut(s) 389, 538, 757
NlaIV GGNNCC 1 cut(s) 403
PdmI GAANNNNTTC 1 cut(s) 419
PfeI GAWTC 1 cut(s) 338
PkrI GCNGC 5 cut(s) 19, 74, 508, 813, 818
PleI GAGTC 2 cut(s) 559, 780
PpsI GAGTC 2 cut(s) 559, 780
Psp6I CCWGG 2 cut(s) 205, 259
PspFI CCCAGC 1 cut(s) 649
PspGI CCWGG 2 cut(s) 205, 259
PspN4I GGNNCC 1 cut(s) 403
PspPI GGNCC 3 cut(s) 408, 489, 642
PsuI RGATCY 4 cut(s) 7, 538, 600, 679
RsaI GTAC 2 cut(s) 258, 765
RsaNI GTAC 2 cut(s) 257, 764
RseI CAYNNNNRTG 1 cut(s) 390
SaqAI TTAA 1 cut(s) 81
SatI GCNGC 5 cut(s) 18, 73, 507, 812, 817
Sau3AI GATC 6 cut(s) 7, 178, 196, 538, 600, 679
Sau96I GGNCC 3 cut(s) 408, 489, 642
SchI GAGTC 2 cut(s) 559, 781
ScrFI CCNGG 6 cut(s) 207, 261, 397, 406, 411, 412
SduI GDGCHC 1 cut(s) 323
SfaNI GCATC 2 cut(s) 534, 687
SinI GGWCC 2 cut(s) 489, 642
SmaI CCCGGG 1 cut(s) 412
SmiMI CAYNNNNRTG 1 cut(s) 390
Sse9I AATT 8 cut(s) 78, 90, 96, 201, 264, 357, 480, 494
SsiI CCGC 1 cut(s) 637
SspMI CTAG 2 cut(s) 363, 548
StyD4I CCNGG 6 cut(s) 205, 259, 395, 404, 409, 410
TaaI ACNGT 3 cut(s) 256, 318, 768
TaiI ACGT 1 cut(s) 394
TaqI TCGA 2 cut(s) 195, 692
TaqII GACCGA 1 cut(s) 659
TasI AATT 8 cut(s) 78, 90, 96, 201, 264, 357, 480, 494
TfiI GAWTC 1 cut(s) 338
Tru1I TTAA 1 cut(s) 81
Tru9I TTAA 1 cut(s) 81
TscAI CASTG 2 cut(s) 323, 439
TseI GCWGC 5 cut(s) 17, 72, 506, 811, 816
TspDTI ATGAA 1 cut(s) 402
TspMI CCCGGG 1 cut(s) 410
TspRI CASTG 2 cut(s) 323, 439
VpaK11BI GGWCC 2 cut(s) 489, 642
XagI CCTNNNNNAGG 1 cut(s) 379
XapI RAATTY 3 cut(s) 201, 480, 494
XmaI CCCGGG 1 cut(s) 410
XmnI GAANNNNTTC 1 cut(s) 419
XspI CTAG 2 cut(s) 363, 548
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.