FvH4_2g21690

Belongs to the iron ascorbate-dependent oxidoreductase family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb2
OG0016759
Physical Location & Seq
Forward (+)
17972393 .. 17974138
1746 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_2g21690.t2

Sequence Viewer

Length: 1029 bp
ATGGATACTGTTTTAGATCAAAAGCTTGTTTCAAGCTGGTTTGATGTTCATCAATCCGTGCCCCAAACATATGTTTTTCCACCCGAAAAACGACCCGGAAAGCTCAAGGTTTCCCTATGCAAGAGCATTCCAGTAGTTGATCTCGGCAGCCATGATCGCCGTGACACCATTCAGCAGATTTTCAAAGCTAGCCAAGACTTTGGTTTTTTCCAGGTTATCAACCATGGAGTTTCGAATAAGTTAATCGATGATACGATGAGTGTTTTCAAGGAGTTCCATGCAATGCCCTCCAAAGACAAGGCAAATGAATGCTCCAAGGACCCGAGTCGGGACTGCAAGCTCTACACAAGTAGTGAAAACTATGCGAATGAAGAAGTTCACTTCTGGAGAGATGCATTGACTCACCCTGGTCATACTTCAGAAAAATACCTGCAGTATTGGCCGCAAAAACCAACTCAGTACCGAGAGATTGTTAAGCGGTATCTGGAGGAGGTGAGGAACATGGGTGCTGTGATTCTGGAGCTGCTTGCTGAAGGGTTGGGACTTGGCGCTGACTTCTTCAATGGGGGACTTAGTGATAGTCCAATGCTGCTGTCAAATCACTATCCGCCATGTCCGGATCCTAGCTTGACATTGGGGTTAACCAAACACCGGGATCCTACCCTTATAACCATTTTACTGCAAGATACAGAAGGGCTTCAAGTTTTCAACGATGGGAATTGGATTGGGGTTGAGCCTGTTTCTACAGCTTTTGTGGTCAACATAGGCTATGTACTCCAGATAATCAGCAACTCGAAGTTTAAGGGTGCAGAACACAGAGTGGTGACTAATTCAAGAGTCGCGAGGACAACCATTGCTTACTTGATTTATCCTTCTAACGACAACCTTATAGAACCTGCAAAAGCTCTATGCAACCGAAATCCGCCACTCTACCGATCGATGACATTTAAGGAGTTCCTTAAAAACTTTAAGTCCAAAGTTGGCCATACTGAATCAGTGTTGCAGACTATCAGCGTCTCAAGTAGCTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
2OG-FeII_Oxy DIOX_N

Protein Analysis

343

Amino Acids

38.65

Weight (kDa)

6.83

Isoelectric Point (pI)

39.97

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DIOX_N PF14226 43 - 148 6e-20 non-haem dioxygenase in morphine synthesis N-terminal
2OG-FeII_Oxy PF03171 195 - 261 8.8e-20 2OG-Fe(II) oxygenase superfamily
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0016759)

Species Orthologous Gene IDs
fragaria_vesca FvH4_2g21690 FvH4_2g21690 FvH4_2g21690
malus_domestica MD05G1199300.v1.1 MD10G1185400.v1.1
prunus_persica Prupe.8G225100_v2.0.a1
rosa_chinensis RchiOBHm_Chr6g0288371
rosa_laevigata RLG00000012410
rosa_multiflora Rmu_sc0001038.1_g000009
rosa_roxburghii Rroxscaffold_7G00180120
rosa_rugosa Rorug06G0196000.1
rosa_samantha Rh6AG306900 Rh6BG312900
rosa_wichuraiana Rw6G026550

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 668
Acc36I ACCTGC 2 cut(s) 438, 904
AccII CGCG 1 cut(s) 842
AccIII TCCGGA 1 cut(s) 616
AciI CCGC 4 cut(s) 443, 478, 608, 923
AclWI GGATC 4 cut(s) 614, 627, 650, 663
AcoI YGGCCR 2 cut(s) 440, 982
AcuI CTGAAG 2 cut(s) 402, 552
AdeI CACNNNGTG 1 cut(s) 820
AfaI GTAC 2 cut(s) 461, 774
AfiI CCNNNNNNNGG 2 cut(s) 328, 651
AgsI TTSAA 7 cut(s) 33, 184, 268, 562, 701, 709, 834
AjnI CCWGG 2 cut(s) 210, 406
Alw26I GTCTC 1 cut(s) 1021
AlwI GGATC 4 cut(s) 614, 627, 650, 663
Ama87I CYCGRG 1 cut(s) 322
Aor13HI TCCGGA 1 cut(s) 616
AoxI GGCC 2 cut(s) 440, 982
ApeKI GCWGC 3 cut(s) 147, 523, 589
Asp700I GAANNNNTTC 2 cut(s) 375, 696
AspLEI GCGC 1 cut(s) 551
AspS9I GGNCC 1 cut(s) 319
AsuC2I CCSGG 2 cut(s) 96, 653
AsuHPI GGTGA 3 cut(s) 395, 505, 835
AsuII TTCGAA 1 cut(s) 233
AsuNHI GCTAGC 1 cut(s) 188
AvaI CYCGRG 1 cut(s) 322
AvaII GGWCC 1 cut(s) 319
BaeGI GKGCMC 1 cut(s) 63
BalI TGGCCA 1 cut(s) 984
BamHI GGATCC 2 cut(s) 619, 655
BbvI GCAGC 3 cut(s) 159, 510, 576
BccI CCATC 1 cut(s) 707
BceAI ACGGC 1 cut(s) 144
BciT130I CCWGG 2 cut(s) 212, 408
BcnI CCSGG 2 cut(s) 96, 653
BcoDI GTCTC 1 cut(s) 1021
BfaI CTAG 2 cut(s) 189, 624
BfmI CTRYAG 2 cut(s) 431, 744
BfoI RGCGCY 1 cut(s) 552
BfuAI ACCTGC 2 cut(s) 438, 904
BisI GCNGC 4 cut(s) 148, 443, 524, 590
BlsI GCNGC 4 cut(s) 149, 444, 525, 591
Bme1390I CCNGG 4 cut(s) 96, 212, 408, 653
Bme18I GGWCC 1 cut(s) 319
BmeT110I CYCGRG 1 cut(s) 322
BmgT120I GGNCC 1 cut(s) 319
BmiI GGNNCC 3 cut(s) 321, 621, 657
BmrFI CCNGG 4 cut(s) 96, 212, 408, 653
BmsI GCATC 1 cut(s) 382
BmtI GCTAGC 1 cut(s) 192
BoxI GACNNNNGTC 1 cut(s) 324
BpmI CTGGAG 4 cut(s) 406, 506, 539, 761
Bpu14I TTCGAA 1 cut(s) 233
BpuEI CTTGAG 2 cut(s) 89, 1003
BpuMI CCSGG 2 cut(s) 96, 653
Bsa29I ATCGAT 2 cut(s) 246, 938
BsaBI GATNNNNATC 1 cut(s) 48
BsaJI CCNNGG 3 cut(s) 223, 315, 406
BsaWI WCCGGW 1 cut(s) 616
Bsc4I CCNNNNNNNGG 2 cut(s) 328, 651
Bse1I ACTGG 1 cut(s) 131
Bse3DI GCAATG 2 cut(s) 288, 852
Bse8I GATNNNNATC 1 cut(s) 48
BseAI TCCGGA 1 cut(s) 616
BseBI CCWGG 2 cut(s) 212, 408
BseCI ATCGAT 2 cut(s) 246, 938
BseDI CCNNGG 3 cut(s) 223, 315, 406
BseJI GATNNNNATC 1 cut(s) 48
BseLI CCNNNNNNNGG 2 cut(s) 328, 651
BseMI GCAATG 2 cut(s) 288, 852
BseMII CTCAG 1 cut(s) 470
BseNI ACTGG 1 cut(s) 131
BseRI GAGGAG 1 cut(s) 503
BseSI GKGCMC 1 cut(s) 63
BseXI GCAGC 3 cut(s) 159, 510, 576
BsgI GTGCAG 1 cut(s) 828
Bsh1236I CGCG 1 cut(s) 842
Bsh1285I CGRYCG 1 cut(s) 938
BshFI GGCC 2 cut(s) 442, 984
BshVI ATCGAT 2 cut(s) 246, 938
BsiEI CGRYCG 1 cut(s) 938
BsiHKCI CYCGRG 1 cut(s) 322
BsiSI CCGG 3 cut(s) 96, 617, 652
BslFI GGGAC 3 cut(s) 344, 555, 582
BslI CCNNNNNNNGG 2 cut(s) 328, 651
BsmAI GTCTC 1 cut(s) 1021
BsmBI CGTCTC 1 cut(s) 1021
BsmFI GGGAC 3 cut(s) 344, 555, 582
BsmI GAATGC 2 cut(s) 126, 314
BsnI GGCC 2 cut(s) 442, 984
BsoBI CYCGRG 1 cut(s) 322
Bsp119I TTCGAA 1 cut(s) 233
Bsp1286I GDGCHC 1 cut(s) 63
Bsp13I TCCGGA 1 cut(s) 616
Bsp143I GATC 6 cut(s) 16, 139, 154, 619, 655, 935
Bsp19I CCATGG 1 cut(s) 223
Bsp68I TCGCGA 1 cut(s) 842
BspACI CCGC 4 cut(s) 443, 478, 608, 923
BspANI GGCC 2 cut(s) 442, 984
BspCNI CTCAG 1 cut(s) 469
BspDI ATCGAT 2 cut(s) 246, 938
BspEI TCCGGA 1 cut(s) 616
BspFNI CGCG 1 cut(s) 842
BspLI GGNNCC 3 cut(s) 321, 621, 657
BspMAI CTGCAG 1 cut(s) 435
BspMI ACCTGC 2 cut(s) 438, 904
BspOI GCTAGC 1 cut(s) 192
BspPI GGATC 4 cut(s) 614, 627, 650, 663
BspT104I TTCGAA 1 cut(s) 233
BsrDI GCAATG 2 cut(s) 288, 852
BsrI ACTGG 1 cut(s) 131
BssECI CCNNGG 3 cut(s) 223, 315, 406
BssMI GATC 6 cut(s) 16, 139, 154, 619, 655, 935
BssT1I CCWWGG 2 cut(s) 223, 315
Bst2UI CCWGG 2 cut(s) 212, 408
Bst4CI ACNGT 1 cut(s) 10
BstBI TTCGAA 1 cut(s) 233
BstC8I GCNNGC 3 cut(s) 190, 338, 528
BstDEI CTNAG 2 cut(s) 456, 572
BstDSI CCRYGG 1 cut(s) 223
BstFNI CGCG 1 cut(s) 842
BstH2I RGCGCY 1 cut(s) 552
BstHHI GCGC 1 cut(s) 551
BstKTI GATC 6 cut(s) 19, 142, 157, 622, 658, 938
BstMAI GTCTC 1 cut(s) 1021
BstMBI GATC 6 cut(s) 16, 139, 154, 619, 655, 935
BstMCI CGRYCG 1 cut(s) 938
BstMWI GCNNNNNNNGC 2 cut(s) 156, 439
BstNI CCWGG 2 cut(s) 212, 408
BstPAI GACNNNNGTC 1 cut(s) 324
BstSCI CCNGG 4 cut(s) 94, 210, 406, 651
BstSFI CTRYAG 2 cut(s) 431, 744
BstSLI GKGCMC 1 cut(s) 63
BstUI CGCG 1 cut(s) 842
BstV1I GCAGC 3 cut(s) 159, 510, 576
BstX2I RGATCY 2 cut(s) 619, 655
BstXI CCANNNNNNTGG 1 cut(s) 200
BstYI RGATCY 2 cut(s) 619, 655
Bsu15I ATCGAT 2 cut(s) 246, 938
BsuRI GGCC 2 cut(s) 442, 984
BsuTUI ATCGAT 2 cut(s) 246, 938
BtgI CCRYGG 1 cut(s) 223
BtsIMutI CAGTG 1 cut(s) 1002
BtuMI TCGCGA 1 cut(s) 842
BveI ACCTGC 2 cut(s) 438, 904
Cac8I GCNNGC 3 cut(s) 190, 338, 528
CfoI GCGC 1 cut(s) 551
Cfr13I GGNCC 1 cut(s) 319
ClaI ATCGAT 2 cut(s) 246, 938
CseI GACGC 1 cut(s) 1003
Csp6I GTAC 2 cut(s) 460, 773
CviAII CATG 5 cut(s) 152, 224, 278, 502, 612
CviQI GTAC 2 cut(s) 460, 773
DdeI CTNAG 2 cut(s) 456, 572
DpnI GATC 6 cut(s) 18, 141, 156, 621, 657, 937
DpnII GATC 6 cut(s) 16, 139, 154, 619, 655, 935
DraIII CACNNNGTG 1 cut(s) 820
EaeI YGGCCR 2 cut(s) 440, 982
EciI GGCGGA 2 cut(s) 597, 912
Eco130I CCWWGG 2 cut(s) 223, 315
Eco47I GGWCC 1 cut(s) 319
Eco57I CTGAAG 2 cut(s) 402, 552
Eco88I CYCGRG 1 cut(s) 322
EcoO109I RGGNCCY 1 cut(s) 319
EcoRII CCWGG 2 cut(s) 210, 406
EcoT14I CCWWGG 2 cut(s) 223, 315
EcoT22I ATGCAT 1 cut(s) 397
ErhI CCWWGG 2 cut(s) 223, 315
Esp3I CGTCTC 1 cut(s) 1021
FaeI CATG 5 cut(s) 155, 227, 281, 505, 615
FaqI GGGAC 3 cut(s) 344, 555, 582
FatI CATG 5 cut(s) 151, 223, 277, 501, 611
FauNDI CATATG 1 cut(s) 70
Fnu4HI GCNGC 4 cut(s) 148, 443, 524, 590
Fsp4HI GCNGC 4 cut(s) 148, 443, 524, 590
FspBI CTAG 2 cut(s) 189, 624
GlaI GCGC 1 cut(s) 550
GluI GCNGC 4 cut(s) 148, 443, 524, 590
GsuI CTGGAG 4 cut(s) 406, 506, 539, 761
HaeII RGCGCY 1 cut(s) 552
HaeIII GGCC 2 cut(s) 442, 984
HapII CCGG 3 cut(s) 96, 617, 652
HgaI GACGC 1 cut(s) 1003
HhaI GCGC 1 cut(s) 551
Hin1II CATG 5 cut(s) 155, 227, 281, 505, 615
Hin6I GCGC 1 cut(s) 549
HinP1I GCGC 1 cut(s) 549
HincII GTYRAC 2 cut(s) 642, 760
HindII GTYRAC 2 cut(s) 642, 760
HindIII AAGCTT 1 cut(s) 23
HinfI GANTC 5 cut(s) 325, 400, 514, 837, 992
HpaI GTTAAC 1 cut(s) 642
HpaII CCGG 3 cut(s) 96, 617, 652
HphI GGTGA 3 cut(s) 395, 505, 835
Hpy166II GTNNAC 3 cut(s) 379, 642, 760
Hpy188I TCNGA 1 cut(s) 421
Hpy188III TCNNGA 8 cut(s) 329, 385, 485, 518, 617, 778, 834, 841
Hpy8I GTNNAC 3 cut(s) 379, 642, 760
HpyAV CCTTC 3 cut(s) 527, 686, 882
HpyCH4III ACNGT 1 cut(s) 10
HpyF10VI GCNNNNNNNGC 2 cut(s) 156, 439
HpyF3I CTNAG 2 cut(s) 456, 572
Hsp92II CATG 5 cut(s) 155, 227, 281, 505, 615
HspAI GCGC 1 cut(s) 549
Kpn2I TCCGGA 1 cut(s) 616
KspAI GTTAAC 1 cut(s) 642
Kzo9I GATC 6 cut(s) 16, 139, 154, 619, 655, 935
LmnI GCTCC 2 cut(s) 317, 520
Lsp1109I GCAGC 3 cut(s) 159, 510, 576
LweI GCATC 1 cut(s) 382
MaeI CTAG 2 cut(s) 189, 624
MaeIII GTNAC 2 cut(s) 161, 823
MalI GATC 6 cut(s) 18, 141, 156, 621, 657, 937
MboI GATC 6 cut(s) 16, 139, 154, 619, 655, 935
MboII GAAGA 2 cut(s) 383, 550
MflI RGATCY 2 cut(s) 619, 655
MhlI GDGCHC 1 cut(s) 63
MlsI TGGCCA 1 cut(s) 984
MluCI AATT 2 cut(s) 718, 829
MluNI TGGCCA 1 cut(s) 984
MlyI GAGTC 3 cut(s) 334, 394, 846
MnlI CCTC 5 cut(s) 298, 481, 484, 489, 837
Mox20I TGGCCA 1 cut(s) 984
Mph1103I ATGCAT 1 cut(s) 397
MroI TCCGGA 1 cut(s) 616
MroXI GAANNNNTTC 2 cut(s) 375, 696
MscI TGGCCA 1 cut(s) 984
MseI TTAA 7 cut(s) 242, 474, 641, 801, 948, 960, 969
Msp20I TGGCCA 1 cut(s) 984
MspI CCGG 3 cut(s) 96, 617, 652
MspR9I CCNGG 4 cut(s) 96, 212, 408, 653
Mva1269I GAATGC 2 cut(s) 126, 314
MvaI CCWGG 2 cut(s) 212, 408
MvnI CGCG 1 cut(s) 842
MwoI GCNNNNNNNGC 2 cut(s) 156, 439
NciI CCSGG 2 cut(s) 96, 653
NcoI CCATGG 1 cut(s) 223
NdeI CATATG 1 cut(s) 70
NdeII GATC 6 cut(s) 16, 139, 154, 619, 655, 935
NheI GCTAGC 1 cut(s) 188
NlaIII CATG 5 cut(s) 155, 227, 281, 505, 615
NlaIV GGNNCC 3 cut(s) 321, 621, 657
NmeAIII GCCGAG 1 cut(s) 123
NmuCI GTSAC 2 cut(s) 161, 823
NruI TCGCGA 1 cut(s) 842
NsiI ATGCAT 1 cut(s) 397
NspV TTCGAA 1 cut(s) 233
PctI GAATGC 2 cut(s) 126, 314
PdmI GAANNNNTTC 2 cut(s) 375, 696
PfeI GAWTC 2 cut(s) 514, 992
PkrI GCNGC 4 cut(s) 149, 444, 525, 591
Ple19I CGATCG 1 cut(s) 938
PleI GAGTC 3 cut(s) 333, 394, 845
PpsI GAGTC 3 cut(s) 333, 394, 845
PpuMI RGGWCCY 1 cut(s) 319
PshAI GACNNNNGTC 1 cut(s) 324
PsiI TTATAA 1 cut(s) 668
Psp5II RGGWCCY 1 cut(s) 319
Psp6I CCWGG 2 cut(s) 210, 406
PspGI CCWGG 2 cut(s) 210, 406
PspN4I GGNNCC 3 cut(s) 321, 621, 657
PspPI GGNCC 1 cut(s) 319
PspPPI RGGWCCY 1 cut(s) 319
PstI CTGCAG 1 cut(s) 435
PsuI RGATCY 2 cut(s) 619, 655
PvuI CGATCG 1 cut(s) 938
RruI TCGCGA 1 cut(s) 842
RsaI GTAC 2 cut(s) 461, 774
RsaNI GTAC 2 cut(s) 460, 773
SaqAI TTAA 7 cut(s) 242, 474, 641, 801, 948, 960, 969
SatI GCNGC 4 cut(s) 148, 443, 524, 590
Sau3AI GATC 6 cut(s) 16, 139, 154, 619, 655, 935
Sau96I GGNCC 1 cut(s) 319
SchI GAGTC 3 cut(s) 334, 394, 846
ScrFI CCNGG 4 cut(s) 96, 212, 408, 653
SduI GDGCHC 1 cut(s) 63
SfaNI GCATC 1 cut(s) 382
SfcI CTRYAG 2 cut(s) 431, 744
SfuI TTCGAA 1 cut(s) 233
SinI GGWCC 1 cut(s) 319
SmlI CTYRAG 2 cut(s) 104, 1018
SmoI CTYRAG 2 cut(s) 104, 1018
Sse9I AATT 2 cut(s) 718, 829
SsiI CCGC 4 cut(s) 443, 478, 608, 923
SspMI CTAG 2 cut(s) 189, 624
StyD4I CCNGG 4 cut(s) 94, 210, 406, 651
StyI CCWWGG 2 cut(s) 223, 315
TaaI ACNGT 1 cut(s) 10
TaqI TCGA 4 cut(s) 233, 246, 794, 938
TasI AATT 2 cut(s) 718, 829
TatI WGTACW 1 cut(s) 772
TauI GCSGC 1 cut(s) 445
TfiI GAWTC 2 cut(s) 514, 992
Tru1I TTAA 7 cut(s) 242, 474, 641, 801, 948, 960, 969
Tru9I TTAA 7 cut(s) 242, 474, 641, 801, 948, 960, 969
TscAI CASTG 1 cut(s) 1002
TseFI GTSAC 2 cut(s) 161, 823
TseI GCWGC 3 cut(s) 147, 523, 589
Tsp45I GTSAC 2 cut(s) 161, 823
TspDTI ATGAA 3 cut(s) 38, 321, 384
TspGWI ACGGA 1 cut(s) 46
TspRI CASTG 1 cut(s) 1002
VpaK11BI GGWCC 1 cut(s) 319
XmnI GAANNNNTTC 2 cut(s) 375, 696
XspI CTAG 2 cut(s) 189, 624
Zsp2I ATGCAT 1 cut(s) 397
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.