MD05G1199300.v1.1

Belongs to the iron ascorbate-dependent oxidoreductase family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr05
OG0016759
Physical Location & Seq
Forward (+)
32712511 .. 32714284
1774 bp
Loading structure...
UTR
Exon/CDS
Intron
MD05G1199300.v1.1.491

Sequence Viewer

Length: 1083 bp
ATGACTTCCTATAAAAAGCCAACTTGCAACGATTTTTGTTACTACTGTGTTTCGAAGGTTGATATGGAGACATTTGACCAGAAGCTTGCTTCAAGCTGGTTTGATGTTCAATCTGTTCCCCAAACCTATGTCCACCCACCAGAAAAGCGGCCTGGCAACACTGTCGGCGTTCCTCCTTGCAAGAACATTCCAGTAGTTGATCTTGGCAACGATGATCGCAGTCACACAATTCAACAAATTTCCAAAGCTAGCCAAGACTTCGGATTTTTCCAGGTCATCAATCATGGGGTTTCCAAGAAGTTGATTGATGACACAATGAGTGTTTCCAAGGAGTTTCATGCAATGCCCCGAAAAGACAAGATAATCGAAGGCTCAAAGGACCCTACCGGAAGCTACAAGTTCTACACAAGCAGTGAAAACTATGCGAATGAAGAGCTTCACTACTGGAGAGATGCATTGGCTCACCCCGCTCATCCTTCTGAAAACAACATTCAGTTTTGGCCTCAAAATCCTACTCGATACCGAGAAGTTTTAAAAACTTACGCGGAGGAGGTAAGAAAGTTGGGTTCTAGGATTTTGGAGATGCTTGCTGAAGGGTTGGGACTGAGCAAAGAGTTCTTCAATGGTGGACTTAGTGAATATCCACGGCTGCTGTCCAACCACTATCCGCCGTGCCCAGATCCTAGTTTAACTTTGGGATTAGCGAAACACCGTGATCCGAGCCTCATTACCATTTTACTGCAAGATTCAGAAGGACTCCAAGTCTTCAAAGATGGGAATTGGATTGGTGTTGAGCCTATTTCTAGTGGCTTTGTTGTTAACATAGGTTACGTCATGCAAATGATCAGCAACTCGAAGTTTAAAGGCGCTGACCATCGAGCCGTGACAAATTCAAGAGCTGCAAGGACAACGGTTGCGTACTTTATTTATCCGTCTAATGAGACTGTTATAGAACCTGCAAAAGCTTTGTGCAATCCACCACTGTACAAATCCATGACGTTTACAGAGTTCCTTCAATACTTCAAATCGAAAGCTGCCAACGACGAAGAACTGTCAAAGGTTTTAAGCCTCTCGGGGAGCTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
2OG-FeII_Oxy DIOX_N

Protein Analysis

361

Amino Acids

40.6

Weight (kDa)

6.56

Isoelectric Point (pI)

40.72

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DIOX_N PF14226 63 - 170 1.5e-20 non-haem dioxygenase in morphine synthesis N-terminal
2OG-FeII_Oxy PF03171 217 - 309 2.3e-27 2OG-Fe(II) oxygenase superfamily
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
iTOL Interactive Tree Of Life · Most popular EvolView Flexible annotations MEGA Desktop phylogenetics suite FigTree Desktop tree editor

Orthologous Genes (Group: OG0016759)

Species Orthologous Gene IDs
fragaria_vesca FvH4_2g21690 FvH4_2g21690 FvH4_2g21690
malus_domestica MD05G1199300.v1.1 MD10G1185400.v1.1
prunus_persica Prupe.8G225100_v2.0.a1
rosa_chinensis RchiOBHm_Chr6g0288371
rosa_laevigata RLG00000012410
rosa_multiflora Rmu_sc0001038.1_g000009
rosa_roxburghii Rroxscaffold_7G00180120
rosa_rugosa Rorug06G0196000.1
rosa_samantha Rh6AG306900 Rh6BG312900
rosa_wichuraiana Rw6G026550

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 964
AccBSI CCGCTC 1 cut(s) 470
AccII CGCG 1 cut(s) 545
AciI CCGC 4 cut(s) 148, 468, 545, 668
AclWI GGATC 2 cut(s) 674, 710
AcsI RAATTY 2 cut(s) 237, 889
AcuI CTGAAG 1 cut(s) 612
AfaI GTAC 2 cut(s) 920, 986
AgsI TTSAA 8 cut(s) 93, 110, 233, 622, 769, 894, 1016, 1024
AhdI GACNNNNNGTC 1 cut(s) 761
AjnI CCWGG 2 cut(s) 151, 270
AluBI AGCT 9 cut(s) 85, 96, 248, 393, 436, 899, 965, 1034, 1080
AluI AGCT 9 cut(s) 85, 96, 248, 393, 436, 899, 965, 1034, 1080
Alw26I GTCTC 2 cut(s) 62, 935
AlwI GGATC 2 cut(s) 674, 710
Ama87I CYCGRG 1 cut(s) 1072
AoxI GGCC 2 cut(s) 149, 500
ApeKI GCWGC 3 cut(s) 649, 899, 1034
ApoI RAATTY 2 cut(s) 237, 889
Asp700I GAANNNNTTC 1 cut(s) 435
AspLEI GCGC 1 cut(s) 869
AspS9I GGNCC 1 cut(s) 379
AsuHPI GGTGA 1 cut(s) 455
AsuII TTCGAA 1 cut(s) 53
AsuNHI GCTAGC 1 cut(s) 248
AvaI CYCGRG 1 cut(s) 1072
AvaII GGWCC 1 cut(s) 379
BaeGI GKGCMC 1 cut(s) 677
BbsI GAAGAC 1 cut(s) 757
BbvI GCAGC 3 cut(s) 636, 886, 1021
BccI CCATC 2 cut(s) 767, 882
BceAI ACGGC 3 cut(s) 655, 662, 866
BcgI CGANNNNNNTGC 2 cut(s) 145, 179
BciT130I CCWGG 2 cut(s) 153, 272
BclI TGATCA 1 cut(s) 843
BcoDI GTCTC 2 cut(s) 62, 935
BfaI CTAG 4 cut(s) 249, 570, 684, 804
BfoI RGCGCY 1 cut(s) 870
BfuAI ACCTGC 1 cut(s) 964
BisI GCNGC 4 cut(s) 149, 650, 900, 1035
BlsI GCNGC 4 cut(s) 150, 651, 901, 1036
Bme1390I CCNGG 2 cut(s) 153, 272
Bme18I GGWCC 1 cut(s) 379
BmeRI GACNNNNNGTC 1 cut(s) 761
BmeT110I CYCGRG 1 cut(s) 1072
BmgT120I GGNCC 1 cut(s) 379
BmiI GGNNCC 1 cut(s) 381
BmrFI CCNGG 2 cut(s) 153, 272
BmsI GCATC 2 cut(s) 442, 573
BmtI GCTAGC 1 cut(s) 252
BpiI GAAGAC 1 cut(s) 757
BpmI CTGGAG 1 cut(s) 466
Bpu14I TTCGAA 1 cut(s) 53
BsaJI CCNNGG 2 cut(s) 327, 644
BsaWI WCCGGW 1 cut(s) 386
Bse1I ACTGG 2 cut(s) 191, 449
Bse3DI GCAATG 1 cut(s) 348
BseBI CCWGG 2 cut(s) 153, 272
BseDI CCNNGG 2 cut(s) 327, 644
BseGI GGATG 1 cut(s) 472
BseMI GCAATG 1 cut(s) 348
BseMII CTCAG 1 cut(s) 596
BseNI ACTGG 2 cut(s) 191, 449
BseRI GAGGAG 1 cut(s) 563
BseSI GKGCMC 1 cut(s) 677
BseXI GCAGC 3 cut(s) 636, 886, 1021
Bsh1236I CGCG 1 cut(s) 545
BshFI GGCC 2 cut(s) 151, 502
BsiHKCI CYCGRG 1 cut(s) 1072
BsiSI CCGG 1 cut(s) 387
BslFI GGGAC 1 cut(s) 615
BsmAI GTCTC 2 cut(s) 62, 935
BsmFI GGGAC 1 cut(s) 615
BsnI GGCC 2 cut(s) 151, 502
BsoBI CYCGRG 1 cut(s) 1072
Bsp119I TTCGAA 1 cut(s) 53
Bsp1286I GDGCHC 1 cut(s) 677
Bsp1407I TGTACA 1 cut(s) 984
Bsp143I GATC 5 cut(s) 199, 214, 679, 715, 843
BspACI CCGC 4 cut(s) 148, 468, 545, 668
BspANI GGCC 2 cut(s) 151, 502
BspCNI CTCAG 1 cut(s) 597
BspFNI CGCG 1 cut(s) 545
BspLI GGNNCC 1 cut(s) 381
BspMI ACCTGC 1 cut(s) 964
BspOI GCTAGC 1 cut(s) 252
BspPI GGATC 2 cut(s) 674, 710
BspQI GCTCTTC 1 cut(s) 426
BspT104I TTCGAA 1 cut(s) 53
BsrBI CCGCTC 1 cut(s) 470
BsrDI GCAATG 1 cut(s) 348
BsrGI TGTACA 1 cut(s) 984
BsrI ACTGG 2 cut(s) 191, 449
BssECI CCNNGG 2 cut(s) 327, 644
BssMI GATC 5 cut(s) 199, 214, 679, 715, 843
BssT1I CCWWGG 1 cut(s) 327
Bst2UI CCWGG 2 cut(s) 153, 272
Bst4CI ACNGT 7 cut(s) 47, 163, 713, 913, 946, 984, 1053
Bst6I CTCTTC 1 cut(s) 426
BstAUI TGTACA 1 cut(s) 984
BstBI TTCGAA 1 cut(s) 53
BstC8I GCNNGC 3 cut(s) 87, 250, 588
BstDEI CTNAG 2 cut(s) 605, 632
BstDSI CCRYGG 1 cut(s) 644
BstF5I GGATG 1 cut(s) 472
BstFNI CGCG 1 cut(s) 545
BstH2I RGCGCY 1 cut(s) 870
BstHHI GCGC 1 cut(s) 869
BstKTI GATC 5 cut(s) 202, 217, 682, 718, 846
BstMAI GTCTC 2 cut(s) 62, 935
BstMBI GATC 5 cut(s) 199, 214, 679, 715, 843
BstMWI GCNNNNNNNGC 1 cut(s) 467
BstNI CCWGG 2 cut(s) 153, 272
BstSCI CCNGG 2 cut(s) 151, 270
BstSLI GKGCMC 1 cut(s) 677
BstUI CGCG 1 cut(s) 545
BstV1I GCAGC 3 cut(s) 636, 886, 1021
BstV2I GAAGAC 1 cut(s) 757
BstX2I RGATCY 1 cut(s) 679
BstYI RGATCY 1 cut(s) 679
BsuRI GGCC 2 cut(s) 151, 502
BtgI CCRYGG 1 cut(s) 644
BtsCI GGATG 1 cut(s) 472
BtsI GCAGTG 1 cut(s) 418
BtsIMutI CAGTG 3 cut(s) 159, 418, 980
BveI ACCTGC 1 cut(s) 964
Cac8I GCNNGC 3 cut(s) 87, 250, 588
CfoI GCGC 1 cut(s) 869
Cfr13I GGNCC 1 cut(s) 379
Csp6I GTAC 2 cut(s) 919, 985
CviAII CATG 4 cut(s) 284, 338, 835, 994
CviQI GTAC 2 cut(s) 919, 985
DdeI CTNAG 2 cut(s) 605, 632
DpnI GATC 5 cut(s) 201, 216, 681, 717, 845
DpnII GATC 5 cut(s) 199, 214, 679, 715, 843
DraI TTTAAA 2 cut(s) 534, 862
DriI GACNNNNNGTC 1 cut(s) 761
Eam1104I CTCTTC 1 cut(s) 426
Eam1105I GACNNNNNGTC 1 cut(s) 761
EarI CTCTTC 1 cut(s) 426
EciI GGCGGA 1 cut(s) 657
Eco130I CCWWGG 1 cut(s) 327
Eco47I GGWCC 1 cut(s) 379
Eco57I CTGAAG 1 cut(s) 612
Eco88I CYCGRG 1 cut(s) 1072
EcoO109I RGGNCCY 1 cut(s) 379
EcoRII CCWGG 2 cut(s) 151, 270
EcoT14I CCWWGG 1 cut(s) 327
EcoT22I ATGCAT 1 cut(s) 457
ErhI CCWWGG 1 cut(s) 327
FaeI CATG 4 cut(s) 287, 341, 838, 997
FaqI GGGAC 1 cut(s) 615
FatI CATG 4 cut(s) 283, 337, 834, 993
FauI CCCGC 1 cut(s) 475
FbaI TGATCA 1 cut(s) 843
Fnu4HI GCNGC 4 cut(s) 149, 650, 900, 1035
FokI GGATG 1 cut(s) 459
Fsp4HI GCNGC 4 cut(s) 149, 650, 900, 1035
FspBI CTAG 4 cut(s) 249, 570, 684, 804
GlaI GCGC 1 cut(s) 868
GluI GCNGC 4 cut(s) 149, 650, 900, 1035
GsuI CTGGAG 1 cut(s) 466
HaeII RGCGCY 1 cut(s) 870
HaeIII GGCC 2 cut(s) 151, 502
HapII CCGG 1 cut(s) 387
HhaI GCGC 1 cut(s) 869
Hin1II CATG 4 cut(s) 287, 341, 838, 997
Hin6I GCGC 1 cut(s) 867
HinP1I GCGC 1 cut(s) 867
HincII GTYRAC 1 cut(s) 820
HindII GTYRAC 1 cut(s) 820
HindIII AAGCTT 2 cut(s) 83, 963
HinfI GANTC 2 cut(s) 746, 756
HpaI GTTAAC 1 cut(s) 820
HpaII CCGG 1 cut(s) 387
HphI GGTGA 1 cut(s) 455
Hpy166II GTNNAC 4 cut(s) 133, 629, 820, 1002
Hpy188I TCNGA 4 cut(s) 263, 481, 720, 751
Hpy188III TCNNGA 1 cut(s) 894
Hpy8I GTNNAC 4 cut(s) 133, 629, 820, 1002
Hpy99I CGWCG 1 cut(s) 1046
HpyAV CCTTC 6 cut(s) 49, 362, 486, 587, 746, 1022
HpyCH4III ACNGT 7 cut(s) 47, 163, 713, 913, 946, 984, 1053
HpyCH4IV ACGT 2 cut(s) 831, 998
HpyCH4V TGCA 9 cut(s) 27, 180, 341, 455, 742, 838, 902, 959, 972
HpyF10VI GCNNNNNNNGC 1 cut(s) 467
HpyF3I CTNAG 2 cut(s) 605, 632
HpySE526I ACGT 2 cut(s) 831, 998
Hsp92II CATG 4 cut(s) 287, 341, 838, 997
HspAI GCGC 1 cut(s) 867
Ksp22I TGATCA 1 cut(s) 843
KspAI GTTAAC 1 cut(s) 820
Kzo9I GATC 5 cut(s) 199, 214, 679, 715, 843
LguI GCTCTTC 1 cut(s) 426
LmnI GCTCC 1 cut(s) 1077
Lsp1109I GCAGC 3 cut(s) 636, 886, 1021
LweI GCATC 2 cut(s) 442, 573
MaeI CTAG 4 cut(s) 249, 570, 684, 804
MaeII ACGT 2 cut(s) 831, 998
MaeIII GTNAC 4 cut(s) 38, 221, 827, 883
MalI GATC 5 cut(s) 201, 216, 681, 717, 845
MbiI CCGCTC 1 cut(s) 470
MboI GATC 5 cut(s) 199, 214, 679, 715, 843
MboII GAAGA 4 cut(s) 443, 610, 757, 1058
MflI RGATCY 1 cut(s) 679
MhlI GDGCHC 1 cut(s) 677
MluCI AATT 4 cut(s) 228, 237, 778, 889
MlyI GAGTC 1 cut(s) 750
MmeI TCCRAC 1 cut(s) 681
MnlI CCTC 6 cut(s) 183, 513, 541, 544, 734, 1079
Mph1103I ATGCAT 1 cut(s) 457
MroXI GAANNNNTTC 1 cut(s) 435
MseI TTAA 5 cut(s) 533, 689, 819, 861, 1064
MslI CAYNNNNRTG 1 cut(s) 839
MspI CCGG 1 cut(s) 387
MspR9I CCNGG 2 cut(s) 153, 272
MvaI CCWGG 2 cut(s) 153, 272
MvnI CGCG 1 cut(s) 545
MwoI GCNNNNNNNGC 1 cut(s) 467
NdeII GATC 5 cut(s) 199, 214, 679, 715, 843
NheI GCTAGC 1 cut(s) 248
NlaIII CATG 4 cut(s) 287, 341, 838, 997
NlaIV GGNNCC 1 cut(s) 381
NmuCI GTSAC 2 cut(s) 221, 883
NsiI ATGCAT 1 cut(s) 457
NspV TTCGAA 1 cut(s) 53
PciSI GCTCTTC 1 cut(s) 426
PdmI GAANNNNTTC 1 cut(s) 435
PfeI GAWTC 1 cut(s) 746
PkrI GCNGC 4 cut(s) 150, 651, 901, 1036
PleI GAGTC 1 cut(s) 750
PpsI GAGTC 1 cut(s) 750
PpuMI RGGWCCY 1 cut(s) 379
Psp5II RGGWCCY 1 cut(s) 379
Psp6I CCWGG 2 cut(s) 151, 270
PspGI CCWGG 2 cut(s) 151, 270
PspN4I GGNNCC 1 cut(s) 381
PspPI GGNCC 1 cut(s) 379
PspPPI RGGWCCY 1 cut(s) 379
PsuI RGATCY 1 cut(s) 679
RsaI GTAC 2 cut(s) 920, 986
RsaNI GTAC 2 cut(s) 919, 985
RseI CAYNNNNRTG 1 cut(s) 839
SapI GCTCTTC 1 cut(s) 426
SaqAI TTAA 5 cut(s) 533, 689, 819, 861, 1064
SatI GCNGC 4 cut(s) 149, 650, 900, 1035
Sau3AI GATC 5 cut(s) 199, 214, 679, 715, 843
Sau96I GGNCC 1 cut(s) 379
SchI GAGTC 1 cut(s) 750
ScrFI CCNGG 2 cut(s) 153, 272
SduI GDGCHC 1 cut(s) 677
SfaNI GCATC 2 cut(s) 442, 573
SfuI TTCGAA 1 cut(s) 53
SinI GGWCC 1 cut(s) 379
SmiMI CAYNNNNRTG 1 cut(s) 839
Sse9I AATT 4 cut(s) 228, 237, 778, 889
SsiI CCGC 4 cut(s) 148, 468, 545, 668
SspMI CTAG 4 cut(s) 249, 570, 684, 804
StyD4I CCNGG 2 cut(s) 151, 270
StyI CCWWGG 1 cut(s) 327
TaaI ACNGT 7 cut(s) 47, 163, 713, 913, 946, 984, 1053
TaiI ACGT 2 cut(s) 834, 1001
TaqI TCGA 6 cut(s) 53, 366, 517, 854, 877, 1028
TasI AATT 4 cut(s) 228, 237, 778, 889
TatI WGTACW 1 cut(s) 984
TauI GCSGC 1 cut(s) 151
TfiI GAWTC 1 cut(s) 746
Tru1I TTAA 5 cut(s) 533, 689, 819, 861, 1064
Tru9I TTAA 5 cut(s) 533, 689, 819, 861, 1064
TscAI CASTG 3 cut(s) 166, 418, 987
TseFI GTSAC 2 cut(s) 221, 883
TseI GCWGC 3 cut(s) 649, 899, 1034
Tsp45I GTSAC 2 cut(s) 221, 883
TspDTI ATGAA 2 cut(s) 326, 444
TspGWI ACGGA 1 cut(s) 921
TspRI CASTG 3 cut(s) 166, 418, 987
VpaK11BI GGWCC 1 cut(s) 379
XapI RAATTY 2 cut(s) 237, 889
XmnI GAANNNNTTC 1 cut(s) 435
XspI CTAG 4 cut(s) 249, 570, 684, 804
Zsp2I ATGCAT 1 cut(s) 457
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.