MD10G1185400.v1.1

Belongs to the iron ascorbate-dependent oxidoreductase family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr10
OG0016759
Physical Location & Seq
Forward (+)
27971966 .. 27973864
1899 bp
Loading structure...
UTR
Exon/CDS
Intron
MD10G1185400.v1.1.491

Sequence Viewer

Length: 1020 bp
ATGGAGACATTAGACCAGAATCTTGTTTCAAGCTGGTTTGATGTTCAATCTGTTCCCCAAACCTTTGTCCACCCACCTGAAAAGCGGCCCGGTAACAGTATTGACGTTCCTCCGTGCAAGAACATTCCGGTAGTTGATCTTGGCAGCCGTGATCGCAGTCACACAATTCAACAAATTTCCAAGGCTAGCCAAGACTTCGGATTTTTCCAGGTCTTCAATCATGGGGTTTGCAAGAAGTTGATTGATGACATAATGAGTATTTCCAAGGAGTTTCATAAAATGCCCCGAAAAGATAAGATAATTGAAGGCTCAAAGGACCCTAGTGGAAGATGCAAGTTCTACACAAGCAGTGAAAACTATGCGAATGAAGAGGTTCACTATTGGCGAGATGCATTGACTCACCCTGCTCATTCTTCTGAAAACTACATGGAGTTTTTGCCTCAAAAACCTACTCAATACCGAGAAGTTTTCAAGGCATACGTGGATGAGGTAAGAAACATGGGTTCTATGATTTTGGAGATGCTTGCTGAAGGGTTGGGACTGAGCAAAGAGTTCTTCAATGGTGGACTTAGTGAAAATCCAACGCTGCTGTCCAACCATTATCCGCCATGCCCAGATCCTACTTTAACCTTGGGATTAACGAAACACCGCGATCCGAGCCTCATAACCATTTTACTTCAAGATTCAGAAGGACTTCAAGTCTTCAAAGATGGGAATTGGATTGGTGTTGAGCCTATTTCTAGTGGCTTTGTTGTTAACATAGGCTACGTCATGCAGATGATCAGCAACTCGAAGTTTAAAGGCGCTGACCATCGAGTGGTGACAAATTCAAGAGCTGCAAGGACAACGATTGCGTACTTCATTTATCCGTCTAATGAGACCGTTATAGAACCTGCAAATGTTTTGTGCAATCCACCACTGTACAGATCCATGAAGTTTACGGAGTTCCTTCAACACTTCAAATCGAAAGCTGCCAACGACGAAGAAATGTCGAAGGTTTTAAGCCTCTCGAGTAGCTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
2OG-FeII_Oxy DIOX_N

Protein Analysis

340

Amino Acids

38.34

Weight (kDa)

6.13

Isoelectric Point (pI)

48.43

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DIOX_N PF14226 42 - 140 2.9e-17 non-haem dioxygenase in morphine synthesis N-terminal
2OG-FeII_Oxy PF03171 194 - 288 2.6e-28 2OG-Fe(II) oxygenase superfamily
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
Loading...
Style Settings
Image
Tree File
Tip: Beautify your tree with professional tools

Download the Full Tree (.nwk) file, then upload it to any of the following tools to customize colors, fonts, annotations, clades, and branch support.

Publication-ready
iTOL Interactive Tree Of Life · Most popular EvolView Flexible annotations MEGA Desktop phylogenetics suite FigTree Desktop tree editor

Orthologous Genes (Group: OG0016759)

Species Orthologous Gene IDs
fragaria_vesca FvH4_2g21690 FvH4_2g21690 FvH4_2g21690
malus_domestica MD05G1199300.v1.1 MD10G1185400.v1.1
prunus_persica Prupe.8G225100_v2.0.a1
rosa_chinensis RchiOBHm_Chr6g0288371
rosa_laevigata RLG00000012410
rosa_multiflora Rmu_sc0001038.1_g000009
rosa_roxburghii Rroxscaffold_7G00180120
rosa_rugosa Rorug06G0196000.1
rosa_samantha Rh6AG306900 Rh6BG312900
rosa_wichuraiana Rw6G026550

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 901
AccB7I CCANNNNNTGG 1 cut(s) 817
AccII CGCG 1 cut(s) 651
AciI CCGC 3 cut(s) 85, 605, 649
AclWI GGATC 3 cut(s) 611, 647, 921
AcsI RAATTY 2 cut(s) 174, 826
AcuI CTGAAG 1 cut(s) 549
AfaI GTAC 2 cut(s) 857, 923
AfiI CCNNNNNNNGG 1 cut(s) 817
AhdI GACNNNNNGTC 1 cut(s) 698
AjnI CCWGG 1 cut(s) 207
AluBI AGCT 4 cut(s) 33, 836, 971, 1017
AluI AGCT 4 cut(s) 33, 836, 971, 1017
Alw26I GTCTC 1 cut(s) 872
AlwI GGATC 3 cut(s) 611, 647, 921
Ama87I CYCGRG 1 cut(s) 1009
AoxI GGCC 1 cut(s) 86
ApeKI GCWGC 4 cut(s) 144, 586, 836, 971
ApoI RAATTY 2 cut(s) 174, 826
Asp700I GAANNNNTTC 2 cut(s) 372, 693
AspLEI GCGC 1 cut(s) 806
AspS9I GGNCC 2 cut(s) 87, 316
AsuC2I CCSGG 1 cut(s) 90
AsuHPI GGTGA 2 cut(s) 392, 832
AsuNHI GCTAGC 1 cut(s) 185
AvaI CYCGRG 1 cut(s) 1009
AvaII GGWCC 1 cut(s) 316
BbsI GAAGAC 2 cut(s) 205, 694
BbvI GCAGC 4 cut(s) 156, 573, 823, 958
BccI CCATC 2 cut(s) 704, 819
BceAI ACGGC 1 cut(s) 132
BciT130I CCWGG 1 cut(s) 209
BclI TGATCA 1 cut(s) 780
BcnI CCSGG 1 cut(s) 90
BcoDI GTCTC 1 cut(s) 872
BfaI CTAG 3 cut(s) 186, 321, 741
BfoI RGCGCY 1 cut(s) 807
BfuAI ACCTGC 1 cut(s) 901
BisI GCNGC 5 cut(s) 86, 145, 587, 837, 972
BlsI GCNGC 5 cut(s) 87, 146, 588, 838, 973
Bme1390I CCNGG 2 cut(s) 90, 209
Bme18I GGWCC 1 cut(s) 316
BmeRI GACNNNNNGTC 1 cut(s) 698
BmeT110I CYCGRG 1 cut(s) 1009
BmgT120I GGNCC 2 cut(s) 87, 316
BmiI GGNNCC 1 cut(s) 318
BmrFI CCNGG 2 cut(s) 90, 209
BmsI GCATC 3 cut(s) 320, 379, 510
BmtI GCTAGC 1 cut(s) 189
BpiI GAAGAC 2 cut(s) 205, 694
BpuMI CCSGG 1 cut(s) 90
BsaAI YACGTR 1 cut(s) 481
BsaI GGTCTC 1 cut(s) 872
BsaJI CCNNGG 3 cut(s) 180, 264, 630
BsaWI WCCGGW 1 cut(s) 127
Bsc4I CCNNNNNNNGG 1 cut(s) 817
BseBI CCWGG 1 cut(s) 209
BseDI CCNNGG 3 cut(s) 180, 264, 630
BseGI GGATG 1 cut(s) 490
BseLI CCNNNNNNNGG 1 cut(s) 817
BseMII CTCAG 1 cut(s) 533
BseXI GCAGC 4 cut(s) 156, 573, 823, 958
Bsh1236I CGCG 1 cut(s) 651
BshFI GGCC 1 cut(s) 88
BsiHKCI CYCGRG 1 cut(s) 1009
BsiSI CCGG 2 cut(s) 90, 128
BslFI GGGAC 1 cut(s) 552
BslI CCNNNNNNNGG 1 cut(s) 817
BsmAI GTCTC 1 cut(s) 872
BsmFI GGGAC 1 cut(s) 552
BsnI GGCC 1 cut(s) 88
Bso31I GGTCTC 1 cut(s) 872
BsoBI CYCGRG 1 cut(s) 1009
Bsp1407I TGTACA 1 cut(s) 921
Bsp143I GATC 6 cut(s) 136, 151, 616, 652, 780, 926
BspACI CCGC 3 cut(s) 85, 605, 649
BspANI GGCC 1 cut(s) 88
BspCNI CTCAG 1 cut(s) 534
BspFNI CGCG 1 cut(s) 651
BspLI GGNNCC 1 cut(s) 318
BspMI ACCTGC 1 cut(s) 901
BspOI GCTAGC 1 cut(s) 189
BspPI GGATC 3 cut(s) 611, 647, 921
BspTNI GGTCTC 1 cut(s) 872
BsrGI TGTACA 1 cut(s) 921
BssECI CCNNGG 3 cut(s) 180, 264, 630
BssMI GATC 6 cut(s) 136, 151, 616, 652, 780, 926
BssT1I CCWWGG 3 cut(s) 180, 264, 630
Bst2UI CCWGG 1 cut(s) 209
Bst4CI ACNGT 3 cut(s) 98, 883, 921
Bst6I CTCTTC 1 cut(s) 363
BstAUI TGTACA 1 cut(s) 921
BstBAI YACGTR 1 cut(s) 481
BstC8I GCNNGC 2 cut(s) 187, 525
BstDEI CTNAG 2 cut(s) 542, 569
BstF5I GGATG 1 cut(s) 490
BstFNI CGCG 1 cut(s) 651
BstH2I RGCGCY 1 cut(s) 807
BstHHI GCGC 1 cut(s) 806
BstKTI GATC 6 cut(s) 139, 154, 619, 655, 783, 929
BstMAI GTCTC 1 cut(s) 872
BstMBI GATC 6 cut(s) 136, 151, 616, 652, 780, 926
BstMWI GCNNNNNNNGC 2 cut(s) 153, 657
BstNI CCWGG 1 cut(s) 209
BstSCI CCNGG 2 cut(s) 88, 207
BstUI CGCG 1 cut(s) 651
BstV1I GCAGC 4 cut(s) 156, 573, 823, 958
BstV2I GAAGAC 2 cut(s) 205, 694
BstX2I RGATCY 2 cut(s) 616, 926
BstYI RGATCY 2 cut(s) 616, 926
BsuRI GGCC 1 cut(s) 88
BtsCI GGATG 1 cut(s) 490
BtsI GCAGTG 1 cut(s) 355
BtsIMutI CAGTG 2 cut(s) 355, 917
BveI ACCTGC 1 cut(s) 901
Cac8I GCNNGC 2 cut(s) 187, 525
CfoI GCGC 1 cut(s) 806
Cfr13I GGNCC 2 cut(s) 87, 316
Csp6I GTAC 2 cut(s) 856, 922
CviAII CATG 6 cut(s) 221, 427, 499, 609, 772, 931
CviQI GTAC 2 cut(s) 856, 922
DdeI CTNAG 2 cut(s) 542, 569
DpnI GATC 6 cut(s) 138, 153, 618, 654, 782, 928
DpnII GATC 6 cut(s) 136, 151, 616, 652, 780, 926
DraI TTTAAA 1 cut(s) 799
DriI GACNNNNNGTC 1 cut(s) 698
Eam1104I CTCTTC 1 cut(s) 363
Eam1105I GACNNNNNGTC 1 cut(s) 698
EarI CTCTTC 1 cut(s) 363
EciI GGCGGA 1 cut(s) 594
Eco130I CCWWGG 3 cut(s) 180, 264, 630
Eco31I GGTCTC 1 cut(s) 872
Eco47I GGWCC 1 cut(s) 316
Eco57I CTGAAG 1 cut(s) 549
Eco88I CYCGRG 1 cut(s) 1009
EcoO109I RGGNCCY 1 cut(s) 316
EcoRII CCWGG 1 cut(s) 207
EcoT14I CCWWGG 3 cut(s) 180, 264, 630
EcoT22I ATGCAT 1 cut(s) 394
ErhI CCWWGG 3 cut(s) 180, 264, 630
FaeI CATG 6 cut(s) 224, 430, 502, 612, 775, 934
FaqI GGGAC 1 cut(s) 552
FatI CATG 6 cut(s) 220, 426, 498, 608, 771, 930
FbaI TGATCA 1 cut(s) 780
Fnu4HI GCNGC 5 cut(s) 86, 145, 587, 837, 972
FokI GGATG 1 cut(s) 497
Fsp4HI GCNGC 5 cut(s) 86, 145, 587, 837, 972
FspBI CTAG 3 cut(s) 186, 321, 741
GlaI GCGC 1 cut(s) 805
GluI GCNGC 5 cut(s) 86, 145, 587, 837, 972
HaeII RGCGCY 1 cut(s) 807
HaeIII GGCC 1 cut(s) 88
HapII CCGG 2 cut(s) 90, 128
HhaI GCGC 1 cut(s) 806
Hin1II CATG 6 cut(s) 224, 430, 502, 612, 775, 934
Hin6I GCGC 1 cut(s) 804
HinP1I GCGC 1 cut(s) 804
HincII GTYRAC 1 cut(s) 757
HindII GTYRAC 1 cut(s) 757
HinfI GANTC 3 cut(s) 19, 397, 683
HpaI GTTAAC 1 cut(s) 757
HpaII CCGG 2 cut(s) 90, 128
HphI GGTGA 2 cut(s) 392, 832
Hpy166II GTNNAC 5 cut(s) 70, 376, 566, 757, 939
Hpy188I TCNGA 4 cut(s) 200, 418, 657, 688
Hpy188III TCNNGA 3 cut(s) 680, 831, 1009
Hpy8I GTNNAC 5 cut(s) 70, 376, 566, 757, 939
Hpy99I CGWCG 1 cut(s) 983
HpyAV CCTTC 5 cut(s) 299, 524, 683, 959, 988
HpyCH4III ACNGT 3 cut(s) 98, 883, 921
HpyCH4IV ACGT 3 cut(s) 105, 480, 768
HpyCH4V TGCA 8 cut(s) 117, 231, 333, 392, 775, 839, 896, 909
HpyF10VI GCNNNNNNNGC 2 cut(s) 153, 657
HpyF3I CTNAG 2 cut(s) 542, 569
HpySE526I ACGT 3 cut(s) 105, 480, 768
Hsp92II CATG 6 cut(s) 224, 430, 502, 612, 775, 934
HspAI GCGC 1 cut(s) 804
Ksp22I TGATCA 1 cut(s) 780
KspAI GTTAAC 1 cut(s) 757
Kzo9I GATC 6 cut(s) 136, 151, 616, 652, 780, 926
Lsp1109I GCAGC 4 cut(s) 156, 573, 823, 958
LweI GCATC 3 cut(s) 320, 379, 510
MaeI CTAG 3 cut(s) 186, 321, 741
MaeII ACGT 3 cut(s) 105, 480, 768
MaeIII GTNAC 3 cut(s) 92, 158, 820
MalI GATC 6 cut(s) 138, 153, 618, 654, 782, 928
MboI GATC 6 cut(s) 136, 151, 616, 652, 780, 926
MboII GAAGA 7 cut(s) 205, 339, 380, 405, 547, 694, 995
MflI RGATCY 2 cut(s) 616, 926
MluCI AATT 5 cut(s) 165, 174, 300, 715, 826
MlyI GAGTC 1 cut(s) 391
MmeI TCCRAC 2 cut(s) 605, 618
MnlI CCTC 6 cut(s) 120, 364, 450, 481, 671, 1016
Mph1103I ATGCAT 1 cut(s) 394
MroXI GAANNNNTTC 2 cut(s) 372, 693
MseI TTAA 5 cut(s) 626, 638, 756, 798, 1001
MslI CAYNNNNRTG 1 cut(s) 776
MspI CCGG 2 cut(s) 90, 128
MspR9I CCNGG 2 cut(s) 90, 209
MvaI CCWGG 1 cut(s) 209
MvnI CGCG 1 cut(s) 651
MwoI GCNNNNNNNGC 2 cut(s) 153, 657
NciI CCSGG 1 cut(s) 90
NdeII GATC 6 cut(s) 136, 151, 616, 652, 780, 926
NheI GCTAGC 1 cut(s) 185
NlaIII CATG 6 cut(s) 224, 430, 502, 612, 775, 934
NlaIV GGNNCC 1 cut(s) 318
NmuCI GTSAC 2 cut(s) 158, 820
NsiI ATGCAT 1 cut(s) 394
PaeR7I CTCGAG 1 cut(s) 1009
PdmI GAANNNNTTC 2 cut(s) 372, 693
PfeI GAWTC 2 cut(s) 19, 683
PflMI CCANNNNNTGG 1 cut(s) 817
PkrI GCNGC 5 cut(s) 87, 146, 588, 838, 973
PleI GAGTC 1 cut(s) 391
PpsI GAGTC 1 cut(s) 391
Ppu21I YACGTR 1 cut(s) 481
PpuMI RGGWCCY 1 cut(s) 316
Psp5II RGGWCCY 1 cut(s) 316
Psp6I CCWGG 1 cut(s) 207
PspGI CCWGG 1 cut(s) 207
PspN4I GGNNCC 1 cut(s) 318
PspPI GGNCC 2 cut(s) 87, 316
PspPPI RGGWCCY 1 cut(s) 316
PsuI RGATCY 2 cut(s) 616, 926
RsaI GTAC 2 cut(s) 857, 923
RsaNI GTAC 2 cut(s) 856, 922
RseI CAYNNNNRTG 1 cut(s) 776
SaqAI TTAA 5 cut(s) 626, 638, 756, 798, 1001
SatI GCNGC 5 cut(s) 86, 145, 587, 837, 972
Sau3AI GATC 6 cut(s) 136, 151, 616, 652, 780, 926
Sau96I GGNCC 2 cut(s) 87, 316
SchI GAGTC 1 cut(s) 391
ScrFI CCNGG 2 cut(s) 90, 209
SfaNI GCATC 3 cut(s) 320, 379, 510
Sfr274I CTCGAG 1 cut(s) 1009
SinI GGWCC 1 cut(s) 316
SlaI CTCGAG 1 cut(s) 1009
SmiMI CAYNNNNRTG 1 cut(s) 776
SmlI CTYRAG 1 cut(s) 1009
SmoI CTYRAG 1 cut(s) 1009
Sse9I AATT 5 cut(s) 165, 174, 300, 715, 826
SsiI CCGC 3 cut(s) 85, 605, 649
SspMI CTAG 3 cut(s) 186, 321, 741
StyD4I CCNGG 2 cut(s) 88, 207
StyI CCWWGG 3 cut(s) 180, 264, 630
TaaI ACNGT 3 cut(s) 98, 883, 921
TaiI ACGT 3 cut(s) 108, 483, 771
TaqI TCGA 5 cut(s) 791, 814, 965, 992, 1010
TasI AATT 5 cut(s) 165, 174, 300, 715, 826
TatI WGTACW 1 cut(s) 921
TauI GCSGC 1 cut(s) 88
TfiI GAWTC 2 cut(s) 19, 683
Tru1I TTAA 5 cut(s) 626, 638, 756, 798, 1001
Tru9I TTAA 5 cut(s) 626, 638, 756, 798, 1001
TscAI CASTG 2 cut(s) 355, 924
TseFI GTSAC 2 cut(s) 158, 820
TseI GCWGC 4 cut(s) 144, 586, 836, 971
Tsp45I GTSAC 2 cut(s) 158, 820
TspDTI ATGAA 4 cut(s) 263, 381, 850, 947
TspGWI ACGGA 3 cut(s) 102, 858, 956
TspRI CASTG 2 cut(s) 355, 924
Van91I CCANNNNNTGG 1 cut(s) 817
VpaK11BI GGWCC 1 cut(s) 316
XapI RAATTY 2 cut(s) 174, 826
XhoI CTCGAG 1 cut(s) 1009
XmnI GAANNNNTTC 2 cut(s) 372, 693
XspI CTAG 3 cut(s) 186, 321, 741
Zsp2I ATGCAT 1 cut(s) 394
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.