FvH4_2g25770

Belongs to the Ca(2 ) cation antiporter (CaCA) (TC 2.A.19) family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb2
OG0007448
Physical Location & Seq
Forward (+)
20815608 .. 20820071
4464 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_2g25770.t3

Sequence Viewer

Length: 1581 bp
ATGCACGTTTCCCTTAGTTGTTCTTGGAATCCACACCCCGTTGGCGCTTTTCTGTCACTCGCCGCAGGTATTCCGGCGGTCGGTTGCGGCGCCGGCAGGTCTCTTCGGCGATTGTCAGCTATTACTGCTCGATTTTTTCGATCCATGGAGAGTGTTGTGAGCCACACGCGGGAGGTTGTGGACGTTGATCCTTGCACTGGTGGTAAAGTTATCAGGCATGAGAAAGTGTGGAACTTCACAATTGCAGACATTAGCTTGCTGGCCTTTGGGACCAGCTTTCCTCAGATATCATTGGCTACTATTGATGCTATACGAAACATTGGGAGCCTCTATGCTGGAGGTTTGGGTCCTGGAACACTAGTTGGTTCTGCTGCATTCGACCTTTTCCCCATCCATGCTGTTTGTGTTGTAGTTCCTAAAGCTGGAGAGCTGAAAAAGATTGCTGATATAGGAGTCTGGCTTGTTGAGCTCTTTTGGTCTTTTTGGGCGTATATTTGGTTATATATTATTTTAGAGGTGTGGACACCAAATGTGATTACACTATGGGAGGCCTTATTGACAGTACTACAATACGGATTACTGCTGACACATGCTTATGCTCAAGACAAGCAGTGGCCCTACTTCTCTCTTCCTATACCAAGAAGTGAGAGACCAGAGGACTGGGTGCCAGCTGAGCCAGTTGCTTGCAAGCTTGATAGTGGTCTCTGTGATGAGTACTCTGAAATACTTCATGTTGGCGAAGATGAAAACAGAGATGTTGTTGATATTTTCTCCATACATTCAGAAACAAGAAATGGTGTAGGTTCAGTGTATCAACAAGTCTCTGGAATTGATGAAAGTCCTGAATCCTCTAATAAGGAGTATCAAGAGAAAATGGTTTCACAAGATCATCCATTTTTTACACTTTGGAGGCAACAATTTGTAGACGCAGTCAAGCTCGAAAGCACAGAATCAAGGAAACTGAGCAACATCTATTTGCGACTGGCAAGAATTTTCTGGCAATTACTTCTTGCTCCTTGGAGACTATTATTTGCATTTGTTCCTCCTTATAACATTGCTCATGGCTGGGTTGCCTTCATAGGTTCCTTAATTTTCATCAGTGCGATAGCTTACATTGTAACACAACTCACTGATCTCATAAGTTGTGTCACAGGAATAAATCCTTATGTCATAGCATTTACTGCATTAGCAGCTGGAACTTCGTGGCCAGATTTAGTTGCAAGTAAGATTGCTGCTGAACGCCAGCTGACAGCTGACTCTGCCATTGCAAACATCACTTGCAGCAATTCGGTGAATATATATGTTGGCATCGGTGTTCCATGGCTGATCGACACTGCATACAATTATATTGTCTACAATGAACCACTGCGGATTGAGAATGCAGCGGGTCTTAGCTTCTCATTGCTTGTATTTTTCGCAACTTCAGTAGGCTGCATTGCTGTTCTGGTAATTAGGCGTTTAACATTGGGTGCAGAGCTTGGAGGGCCAAAGCTTTGGGCATGGATTACCTTTGTGTTTTTCATGTTGCTTTGGATAACTTTTGTTGTACTGTCTTCCCTAAAAGTTTCTGGCATCATATAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0003008 GO:0003674 GO:0005215 GO:0005432 GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005739 GO:0005856 GO:0005874 GO:0005886 GO:0005887 GO:0006810 GO:0006811 GO:0006812 GO:0006814 GO:0006816 GO:0006873 GO:0006874 GO:0006875 GO:0007204 GO:0007275 GO:0007399 GO:0007417 GO:0007420 GO:0007584 GO:0007610 GO:0007611 GO:0007612 GO:0007613 GO:0008150 GO:0008324 GO:0009605 GO:0009987 GO:0009991 GO:0010033 GO:0010646 GO:0010647 GO:0014070 GO:0015075 GO:0015077 GO:0015081 GO:0015085 GO:0015291 GO:0015297 GO:0015298 GO:0015318 GO:0015368 GO:0015491 GO:0015630 GO:0015672 GO:0016020 GO:0016021 GO:0019725 GO:0021537 GO:0022804 GO:0022857 GO:0022890 GO:0023051 GO:0023056 GO:0030001 GO:0030003 GO:0030425 GO:0030900 GO:0031224 GO:0031226 GO:0031667 GO:0032501 GO:0032502 GO:0033273 GO:0033280 GO:0033993 GO:0034220 GO:0035725 GO:0036477 GO:0042221 GO:0042592 GO:0042995 GO:0043005 GO:0043025 GO:0043197 GO:0043226 GO:0043227 GO:0043228 GO:0043229 GO:0043231 GO:0043232 GO:0044297 GO:0044309 GO:0044422 GO:0044424 GO:0044425 GO:0044430 GO:0044444 GO:0044446 GO:0044456 GO:0044459 GO:0044463 GO:0044464 GO:0045202 GO:0046873 GO:0048167 GO:0048168 GO:0048172 GO:0048513 GO:0048518 GO:0048522 GO:0048731 GO:0048856 GO:0048878 GO:0050789 GO:0050794 GO:0050801 GO:0050804 GO:0050806 GO:0050877 GO:0050890 GO:0050896 GO:0051179 GO:0051234 GO:0051480 GO:0051641 GO:0051649 GO:0055065 GO:0055074 GO:0055080 GO:0055082 GO:0055085 GO:0060291 GO:0060322 GO:0060401 GO:0060402 GO:0065007 GO:0065008 GO:0070509 GO:0070588 GO:0070838 GO:0071944 GO:0072503 GO:0072507 GO:0072511 GO:0097447 GO:0097458 GO:0097553 GO:0098655 GO:0098657 GO:0098659 GO:0098660 GO:0098662 GO:0098703 GO:0098739 GO:0098771 GO:0098794 GO:0099080 GO:0099081 GO:0099177 GO:0099512 GO:0099513 GO:0099516 GO:0099587 GO:0120025 GO:0120038 GO:1901660 GO:1901700 GO:1902656 GO:1990034
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Na_Ca_ex

Protein Analysis

527

Amino Acids

58.24

Weight (kDa)

5.52

Isoelectric Point (pI)

39.2

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Na_Ca_ex PF01699 34 - 205 4.2e-16 Sodium/calcium exchanger protein
Na_Ca_ex PF01699 359 - 523 7.2e-23 Sodium/calcium exchanger protein
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 1050
Acc36I ACCTGC 2 cut(s) 56, 87
AccB1I GGYRCC 2 cut(s) 89, 664
AccI GTMKAC 2 cut(s) 924, 1353
AccII CGCG 1 cut(s) 169
AciI CCGC 6 cut(s) 63, 77, 87, 169, 1369, 1385
AclWI GGATC 2 cut(s) 135, 182
AcoI YGGCCR 1 cut(s) 1205
AcsI RAATTY 1 cut(s) 990
AcuI CTGAAG 1 cut(s) 1407
AcyI GRCGYC 1 cut(s) 90
AfaI GTAC 3 cut(s) 564, 716, 1548
AfiI CCNNNNNNNGG 4 cut(s) 80, 169, 197, 422
AhlI ACTAGT 1 cut(s) 358
AjnI CCWGG 1 cut(s) 349
Alw21I GWGCWC 1 cut(s) 471
Alw26I GTCTC 5 cut(s) 105, 643, 707, 826, 1015
AlwI GGATC 2 cut(s) 135, 182
AoxI GGCC 5 cut(s) 261, 549, 614, 1205, 1484
ApeKI GCWGC 6 cut(s) 371, 1190, 1232, 1281, 1382, 1431
ApoI RAATTY 1 cut(s) 990
Asp700I GAANNNNTTC 1 cut(s) 726
AspLEI GCGC 2 cut(s) 47, 92
AspS9I GGNCC 4 cut(s) 270, 347, 615, 1484
AsuHPI GGTGA 1 cut(s) 1303
AvaII GGWCC 2 cut(s) 270, 347
BalI TGGCCA 1 cut(s) 1207
BanI GGYRCC 2 cut(s) 89, 664
BanII GRGCYC 1 cut(s) 471
BbsI GAAGAC 1 cut(s) 1545
Bbv12I GWGCWC 1 cut(s) 471
BbvI GCAGC 6 cut(s) 358, 1202, 1219, 1293, 1394, 1418
BccI CCATC 1 cut(s) 398
BciT130I CCWGG 1 cut(s) 351
BcoDI GTCTC 5 cut(s) 105, 643, 707, 826, 1015
BcuI ACTAGT 1 cut(s) 358
BfaI CTAG 1 cut(s) 359
BfoI RGCGCY 2 cut(s) 48, 93
BfuAI ACCTGC 2 cut(s) 56, 87
BisI GCNGC 8 cut(s) 63, 88, 372, 1191, 1233, 1282, 1383, 1432
BlpI GCTNAGC 1 cut(s) 672
BlsI GCNGC 8 cut(s) 64, 89, 373, 1192, 1234, 1283, 1384, 1433
BmcAI AGTACT 2 cut(s) 564, 716
Bme1390I CCNGG 1 cut(s) 351
Bme18I GGWCC 2 cut(s) 270, 347
BmgT120I GGNCC 4 cut(s) 270, 347, 615, 1484
BmiI GGNNCC 6 cut(s) 91, 271, 326, 348, 666, 1084
BmrFI CCNGG 1 cut(s) 351
BmrI ACTGGG 1 cut(s) 670
BmsI GCATC 2 cut(s) 295, 1317
BmuI ACTGGG 1 cut(s) 670
BpiI GAAGAC 1 cut(s) 1545
BpmI CTGGAG 2 cut(s) 357, 444
Bpu1102I GCTNAGC 1 cut(s) 672
BpuEI CTTGAG 1 cut(s) 585
BsaHI GRCGYC 1 cut(s) 90
BsaI GGTCTC 3 cut(s) 105, 643, 707
BsaJI CCNNGG 3 cut(s) 144, 1016, 1319
Bsc4I CCNNNNNNNGG 4 cut(s) 80, 169, 197, 422
Bse118I RCCGGY 1 cut(s) 92
Bse1I ACTGG 4 cut(s) 202, 665, 677, 987
Bse3DI GCAATG 4 cut(s) 1053, 1263, 1400, 1434
BseBI CCWGG 1 cut(s) 351
BseDI CCNNGG 3 cut(s) 144, 1016, 1319
BseGI GGATG 2 cut(s) 390, 889
BseLI CCNNNNNNNGG 4 cut(s) 80, 169, 197, 422
BseMI GCAATG 4 cut(s) 1053, 1263, 1400, 1434
BseMII CTCAG 3 cut(s) 296, 663, 953
BseNI ACTGG 4 cut(s) 202, 665, 677, 987
BseXI GCAGC 6 cut(s) 358, 1202, 1219, 1293, 1394, 1418
BseYI CCCAGC 1 cut(s) 1065
BsgI GTGCAG 1 cut(s) 1491
Bsh1236I CGCG 1 cut(s) 169
Bsh1285I CGRYCG 1 cut(s) 81
BshFI GGCC 5 cut(s) 263, 551, 616, 1207, 1486
BshNI GGYRCC 2 cut(s) 89, 664
BsiEI CGRYCG 1 cut(s) 81
BsiHKAI GWGCWC 1 cut(s) 471
BsiSI CCGG 2 cut(s) 74, 93
BslFI GGGAC 1 cut(s) 283
BslI CCNNNNNNNGG 4 cut(s) 80, 169, 197, 422
BsmAI GTCTC 5 cut(s) 105, 643, 707, 826, 1015
BsmFI GGGAC 1 cut(s) 283
BsmI GAATGC 2 cut(s) 374, 1384
BsnI GGCC 5 cut(s) 263, 551, 616, 1207, 1486
Bso31I GGTCTC 3 cut(s) 105, 643, 707
Bsp1286I GDGCHC 1 cut(s) 471
Bsp143I GATC 5 cut(s) 140, 187, 886, 1132, 1326
Bsp1720I GCTNAGC 1 cut(s) 672
Bsp19I CCATGG 2 cut(s) 144, 1319
BspACI CCGC 6 cut(s) 63, 77, 87, 169, 1369, 1385
BspANI GGCC 5 cut(s) 263, 551, 616, 1207, 1486
BspCNI CTCAG 3 cut(s) 295, 664, 954
BspFNI CGCG 1 cut(s) 169
BspLI GGNNCC 6 cut(s) 91, 271, 326, 348, 666, 1084
BspMI ACCTGC 2 cut(s) 56, 87
BspPI GGATC 2 cut(s) 135, 182
BspT107I GGYRCC 2 cut(s) 89, 664
BspTNI GGTCTC 3 cut(s) 105, 643, 707
BsrDI GCAATG 4 cut(s) 1053, 1263, 1400, 1434
BsrFI RCCGGY 1 cut(s) 92
BsrI ACTGG 4 cut(s) 202, 665, 677, 987
BssAI RCCGGY 1 cut(s) 92
BssECI CCNNGG 3 cut(s) 144, 1016, 1319
BssMI GATC 5 cut(s) 140, 187, 886, 1132, 1326
BssNI GRCGYC 1 cut(s) 90
BssT1I CCWWGG 3 cut(s) 144, 1016, 1319
Bst2UI CCWGG 1 cut(s) 351
Bst4CI ACNGT 2 cut(s) 562, 1551
Bst6I CTCTTC 2 cut(s) 108, 633
BstACI GRCGYC 1 cut(s) 90
BstAPI GCANNNNNTGC 1 cut(s) 1181
BstC8I GCNNGC 7 cut(s) 94, 257, 261, 669, 685, 689, 1244
BstDEI CTNAG 5 cut(s) 14, 282, 672, 962, 1391
BstDSI CCRYGG 2 cut(s) 144, 1319
BstF5I GGATG 2 cut(s) 390, 889
BstFNI CGCG 1 cut(s) 169
BstH2I RGCGCY 2 cut(s) 48, 93
BstHHI GCGC 2 cut(s) 47, 92
BstKTI GATC 5 cut(s) 143, 190, 889, 1135, 1329
BstMAI GTCTC 5 cut(s) 105, 643, 707, 826, 1015
BstMBI GATC 5 cut(s) 140, 187, 886, 1132, 1326
BstMCI CGRYCG 1 cut(s) 81
BstMWI GCNNNNNNNGC 8 cut(s) 93, 125, 466, 673, 1181, 1190, 1259, 1483
BstNI CCWGG 1 cut(s) 351
BstNSI RCATGY 1 cut(s) 593
BstSCI CCNGG 1 cut(s) 349
BstUI CGCG 1 cut(s) 169
BstV1I GCAGC 6 cut(s) 358, 1202, 1219, 1293, 1394, 1418
BstV2I GAAGAC 1 cut(s) 1545
BstXI CCANNNNNNTGG 2 cut(s) 660, 1494
BsuRI GGCC 5 cut(s) 263, 551, 616, 1207, 1486
BtgI CCRYGG 2 cut(s) 144, 1319
BtsCI GGATG 2 cut(s) 390, 889
BtsI GCAGTG 3 cut(s) 617, 1332, 1364
BtsIMutI CAGTG 7 cut(s) 195, 617, 813, 1105, 1128, 1332, 1364
BveI ACCTGC 2 cut(s) 56, 87
Cac8I GCNNGC 7 cut(s) 94, 257, 261, 669, 685, 689, 1244
CfoI GCGC 2 cut(s) 47, 92
Cfr10I RCCGGY 1 cut(s) 92
Cfr13I GGNCC 4 cut(s) 270, 347, 615, 1484
CseI GACGC 1 cut(s) 935
Csp6I GTAC 3 cut(s) 563, 715, 1547
CviAII CATG 9 cut(s) 145, 218, 395, 590, 731, 1061, 1320, 1500, 1522
CviQI GTAC 3 cut(s) 563, 715, 1547
DdeI CTNAG 5 cut(s) 14, 282, 672, 962, 1391
DinI GGCGCC 1 cut(s) 91
DpnI GATC 5 cut(s) 142, 189, 888, 1134, 1328
DpnII GATC 5 cut(s) 140, 187, 886, 1132, 1326
EaeI YGGCCR 1 cut(s) 1205
Eam1104I CTCTTC 2 cut(s) 108, 633
EarI CTCTTC 2 cut(s) 108, 633
Ecl136II GAGCTC 1 cut(s) 469
Eco130I CCWWGG 3 cut(s) 144, 1016, 1319
Eco147I AGGCCT 1 cut(s) 551
Eco24I GRGCYC 1 cut(s) 471
Eco31I GGTCTC 3 cut(s) 105, 643, 707
Eco32I GATATC 1 cut(s) 288
Eco47I GGWCC 2 cut(s) 270, 347
Eco53kI GAGCTC 1 cut(s) 469
Eco57I CTGAAG 1 cut(s) 1407
EcoICRI GAGCTC 1 cut(s) 469
EcoO109I RGGNCCY 1 cut(s) 347
EcoRII CCWGG 1 cut(s) 349
EcoRV GATATC 1 cut(s) 288
EcoT14I CCWWGG 3 cut(s) 144, 1016, 1319
EcoT38I GRGCYC 1 cut(s) 471
EgeI GGCGCC 1 cut(s) 91
EheI GGCGCC 1 cut(s) 91
ErhI CCWWGG 3 cut(s) 144, 1016, 1319
FaeI CATG 9 cut(s) 148, 221, 398, 593, 734, 1064, 1323, 1503, 1525
FaqI GGGAC 1 cut(s) 283
FatI CATG 9 cut(s) 144, 217, 394, 589, 730, 1060, 1319, 1499, 1521
FauI CCCGC 2 cut(s) 162, 1378
FblI GTMKAC 2 cut(s) 924, 1353
Fnu4HI GCNGC 8 cut(s) 63, 88, 372, 1191, 1233, 1282, 1383, 1432
FokI GGATG 2 cut(s) 377, 876
FriOI GRGCYC 1 cut(s) 471
Fsp4HI GCNGC 8 cut(s) 63, 88, 372, 1191, 1233, 1282, 1383, 1432
FspBI CTAG 1 cut(s) 359
GlaI GCGC 2 cut(s) 46, 91
GluI GCNGC 8 cut(s) 63, 88, 372, 1191, 1233, 1282, 1383, 1432
GsaI CCCAGC 1 cut(s) 1069
GsuI CTGGAG 2 cut(s) 357, 444
HaeII RGCGCY 2 cut(s) 48, 93
HaeIII GGCC 5 cut(s) 263, 551, 616, 1207, 1486
HapII CCGG 2 cut(s) 74, 93
HgaI GACGC 1 cut(s) 935
HhaI GCGC 2 cut(s) 47, 92
Hin1I GRCGYC 1 cut(s) 90
Hin1II CATG 9 cut(s) 148, 221, 398, 593, 734, 1064, 1323, 1503, 1525
Hin6I GCGC 2 cut(s) 45, 90
HinP1I GCGC 2 cut(s) 45, 90
HindIII AAGCTT 2 cut(s) 689, 1490
HinfI GANTC 5 cut(s) 28, 453, 845, 950, 1256
HpaII CCGG 2 cut(s) 74, 93
HphI GGTGA 1 cut(s) 1303
Hpy166II GTNNAC 4 cut(s) 181, 522, 925, 1354
Hpy188I TCNGA 3 cut(s) 285, 721, 784
Hpy188III TCNNGA 4 cut(s) 602, 825, 842, 866
Hpy8I GTNNAC 4 cut(s) 181, 522, 925, 1354
HpyAV CCTTC 1 cut(s) 1084
HpyCH4III ACNGT 2 cut(s) 562, 1551
HpyCH4IV ACGT 2 cut(s) 6, 183
HpyF10VI GCNNNNNNNGC 8 cut(s) 93, 125, 466, 673, 1181, 1190, 1259, 1483
HpyF3I CTNAG 5 cut(s) 14, 282, 672, 962, 1391
HpySE526I ACGT 2 cut(s) 6, 183
Hsp92I GRCGYC 1 cut(s) 90
Hsp92II CATG 9 cut(s) 148, 221, 398, 593, 734, 1064, 1323, 1503, 1525
HspAI GCGC 2 cut(s) 45, 90
KasI GGCGCC 1 cut(s) 89
KroI GCCGGC 1 cut(s) 92
KroNI GCCGGC 1 cut(s) 94
Kzo9I GATC 5 cut(s) 140, 187, 886, 1132, 1326
LmnI GCTCC 2 cut(s) 324, 1018
Lsp1109I GCAGC 6 cut(s) 358, 1202, 1219, 1293, 1394, 1418
LweI GCATC 2 cut(s) 295, 1317
MaeI CTAG 1 cut(s) 359
MaeII ACGT 2 cut(s) 6, 183
MaeIII GTNAC 3 cut(s) 54, 1117, 1147
MalI GATC 5 cut(s) 142, 189, 888, 1134, 1328
MboI GATC 5 cut(s) 140, 187, 886, 1132, 1326
MboII GAAGA 4 cut(s) 95, 620, 752, 1545
MfeI CAATTG 1 cut(s) 240
MhlI GDGCHC 1 cut(s) 471
MlsI TGGCCA 1 cut(s) 1207
MluCI AATT 9 cut(s) 240, 828, 917, 990, 1001, 1089, 1285, 1342, 1449
MluNI TGGCCA 1 cut(s) 1207
Mly113I GGCGCC 1 cut(s) 90
MlyI GAGTC 2 cut(s) 462, 1250
Mox20I TGGCCA 1 cut(s) 1207
MroNI GCCGGC 1 cut(s) 92
MroXI GAANNNNTTC 1 cut(s) 726
MscI TGGCCA 1 cut(s) 1207
MseI TTAA 2 cut(s) 1088, 1460
MslI CAYNNNNRTG 1 cut(s) 594
Msp20I TGGCCA 1 cut(s) 1207
MspA1I CMGCKG 5 cut(s) 671, 1193, 1246, 1253, 1385
MspI CCGG 2 cut(s) 74, 93
MspR9I CCNGG 1 cut(s) 351
MunI CAATTG 1 cut(s) 240
Mva1269I GAATGC 2 cut(s) 374, 1384
MvaI CCWGG 1 cut(s) 351
MvnI CGCG 1 cut(s) 169
MwoI GCNNNNNNNGC 8 cut(s) 93, 125, 466, 673, 1181, 1190, 1259, 1483
NaeI GCCGGC 1 cut(s) 94
NarI GGCGCC 1 cut(s) 90
NcoI CCATGG 2 cut(s) 144, 1319
NdeII GATC 5 cut(s) 140, 187, 886, 1132, 1326
NgoMIV GCCGGC 1 cut(s) 92
NlaIII CATG 9 cut(s) 148, 221, 398, 593, 734, 1064, 1323, 1503, 1525
NlaIV GGNNCC 6 cut(s) 91, 271, 326, 348, 666, 1084
NmuCI GTSAC 2 cut(s) 54, 1147
NspI RCATGY 1 cut(s) 593
PceI AGGCCT 1 cut(s) 551
PcsI WCGNNNNNNNCGW 1 cut(s) 136
PctI GAATGC 2 cut(s) 374, 1384
PdiI GCCGGC 1 cut(s) 94
PdmI GAANNNNTTC 1 cut(s) 726
PfeI GAWTC 3 cut(s) 28, 845, 950
PflFI GACNNNGTC 1 cut(s) 929
PfoI TCCNGGA 1 cut(s) 349
PkrI GCNGC 8 cut(s) 64, 89, 373, 1192, 1234, 1283, 1384, 1433
PleI GAGTC 2 cut(s) 461, 1250
PluTI GGCGCC 1 cut(s) 93
PpsI GAGTC 2 cut(s) 461, 1250
PpuMI RGGWCCY 1 cut(s) 347
PsiI TTATAA 1 cut(s) 1050
Psp124BI GAGCTC 1 cut(s) 471
Psp5II RGGWCCY 1 cut(s) 347
Psp6I CCWGG 1 cut(s) 349
PspFI CCCAGC 1 cut(s) 1065
PspGI CCWGG 1 cut(s) 349
PspN4I GGNNCC 6 cut(s) 91, 271, 326, 348, 666, 1084
PspPI GGNCC 4 cut(s) 270, 347, 615, 1484
PspPPI RGGWCCY 1 cut(s) 347
PsyI GACNNNGTC 1 cut(s) 929
PvuII CAGCTG 4 cut(s) 671, 1193, 1246, 1253
RsaI GTAC 3 cut(s) 564, 716, 1548
RsaNI GTAC 3 cut(s) 563, 715, 1547
RseI CAYNNNNRTG 1 cut(s) 594
SacI GAGCTC 1 cut(s) 471
SaqAI TTAA 2 cut(s) 1088, 1460
SatI GCNGC 8 cut(s) 63, 88, 372, 1191, 1233, 1282, 1383, 1432
Sau3AI GATC 5 cut(s) 140, 187, 886, 1132, 1326
Sau96I GGNCC 4 cut(s) 270, 347, 615, 1484
ScaI AGTACT 2 cut(s) 564, 716
SchI GAGTC 2 cut(s) 462, 1250
ScrFI CCNGG 1 cut(s) 351
SduI GDGCHC 1 cut(s) 471
SfaNI GCATC 2 cut(s) 295, 1317
SfoI GGCGCC 1 cut(s) 91
SinI GGWCC 2 cut(s) 270, 347
SmiMI CAYNNNNRTG 1 cut(s) 594
SmlI CTYRAG 1 cut(s) 600
SmoI CTYRAG 1 cut(s) 600
SpeI ACTAGT 1 cut(s) 358
Sse9I AATT 9 cut(s) 240, 828, 917, 990, 1001, 1089, 1285, 1342, 1449
SseBI AGGCCT 1 cut(s) 551
SsiI CCGC 6 cut(s) 63, 77, 87, 169, 1369, 1385
SspDI GGCGCC 1 cut(s) 89
SspMI CTAG 1 cut(s) 359
SstI GAGCTC 1 cut(s) 471
StuI AGGCCT 1 cut(s) 551
StyD4I CCNGG 1 cut(s) 349
StyI CCWWGG 3 cut(s) 144, 1016, 1319
TaaI ACNGT 2 cut(s) 562, 1551
TaiI ACGT 2 cut(s) 9, 186
TaqI TCGA 5 cut(s) 130, 139, 378, 939, 1329
TasI AATT 9 cut(s) 240, 828, 917, 990, 1001, 1089, 1285, 1342, 1449
TatI WGTACW 3 cut(s) 562, 714, 1546
TauI GCSGC 2 cut(s) 65, 90
TfiI GAWTC 3 cut(s) 28, 845, 950
Tru1I TTAA 2 cut(s) 1088, 1460
Tru9I TTAA 2 cut(s) 1088, 1460
TscAI CASTG 7 cut(s) 202, 617, 813, 1105, 1135, 1339, 1371
TseFI GTSAC 2 cut(s) 54, 1147
TseI GCWGC 6 cut(s) 371, 1190, 1232, 1281, 1382, 1431
Tsp45I GTSAC 2 cut(s) 54, 1147
TspDTI ATGAA 7 cut(s) 719, 759, 849, 1066, 1084, 1374, 1510
TspGWI ACGGA 1 cut(s) 588
TspRI CASTG 7 cut(s) 202, 617, 813, 1105, 1135, 1339, 1371
Tth111I GACNNNGTC 1 cut(s) 929
VpaK11BI GGWCC 2 cut(s) 270, 347
XapI RAATTY 1 cut(s) 990
XceI RCATGY 1 cut(s) 593
XmiI GTMKAC 2 cut(s) 924, 1353
XmnI GAANNNNTTC 1 cut(s) 726
XspI CTAG 1 cut(s) 359
ZrmI AGTACT 2 cut(s) 564, 716
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.