Basic Information

Type: gene

Biological Identity

Species

fragaria_vesca

Chromosome

Fvb2

Orthogroup ID

OG0014882

Physical Location & Seq

Genomic Coordinates Forward (+)

20940015 .. 20944566

Gene Structure 4552 bp

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UTR

Exon/CDS

Intron

Transcript / Protein ID

FvH4_2g25980.t1

Sequence Viewer

CDS Protein Promoter (2k)

Length: 1704 bp

ATGTATAGTCTAAGAAGGAGAACAAAGCTCTTGCTCTCCTTGCTCCCAACTTCAGCCTTTATCCTCATCATATTCTTTTCTCTAAACCACTTCACAAATAGCCCCAAACCCACAAAAGAATTTACCATCCATGTCGAGAAGCACAACCAACTGAACCAAGTAGCTCACTCAACATGCCAAAACACACTCTACAAAGACCTCTGCGTCTCGACCCTGACCTCATTCCCGAATCTGAGCTCGAAAACTGTCCCGCAAATCATATCCTCCACCGTCAACAAGACCATGTACGAGGTCATAGCCTCCAACTCCAACTGCACCGGCATCAAAAAGCACAACAAGAACAAGCTCGCCGCCGTTCAGCAGCAAGCTCTCGACGACTGTCTTGAGCTCTTCGACGACACCGTCGCGCAGCTCAAGGTCACCATGTCGGAACTCAGCTCCAAGAAGTCGCCGGCGGCCAAGCACTACAACCACTTGAGGACGCTGCTCAGCGCCGCAATGACGAACCAGTACACGTGTCTCGAAGGGTTTGATTCCAGCACTAGTAGTACGGTTAAAGCTGTGAAGGCTGAACTGGAGAGCAGATTGAACACTACCTGCAAGCACTTCAGCAATGCGCTTGTGATGCTCAAGAAGATTCGGGGAGTAGTTGAGCGTGAGAGTACTGACGAGTTCGGAGCCATGAAAAACGGGTTCCCTACATGGGTCGGATCCAAAGCTCGGAAGCTGTTGCAGACTAGTTCGGCAAACCAGACCAAGTATGATCTGCTGGTGGCGAAAGACGGTAGCGGAAACTTCACCACCATCACCGACGCGGTGGCTGCGGCTCCAAACTCGAGCGCAACCAGGTTTGTCATATACATAAAAGCAGGTGCGTACTTTGAGAATGTAGAGGTTGTGAGCAAGAAGACGAATTTGATGTTTTTGGGTGATGGAATTGGGAAGACGGTTGTGAAAGCGAGTAGGAATGTTGTCGACGGCTGGACTACTTTCCGATCGGCCACTGTCGCCGTCGTCGGAGGAGGGTTCATCGCCAAAGGCATAACGTTCGAGAACTCAGCCGGACCAAGCAAGCACCAAGCAGTAGCCCTCCGCTCCGGCTCCGACCTCTCCGCCTTCTACCAATGCAGCTTCGTCGGCTACCAAGACACTCTCTACGTCCACTCCCTCCGCCAGTTCTACCGCGAATGCGACATCTACGGCACCGTCGACTTCATCTTCGGCAACGCCGCCGTCGTCTTCCAAAACTGCAACCTCTACGCCCGGAAACCCAACGACGGCCAGAAGAACATCTTCACCGCCCAGGGCCGCGAGGACCCCAACCAGAACACCGGAATCTCCATCCTTAACTGCAAGGTCACCGCCGCGTCGGACCTCATTCCGGTCAAGTCAACGTTCAAGACATATCTCGGCCGTCCGTGGAAGGAGTACTCCCGGACGGTTTACCTGAGGTCGAATATCGACGACACTGTGGACCCCGCCGGGTGGCTGGAGTGGAATGGGACGTTTGCATTGAGCACGTTGTATTATGGGGAATATTTGAACCGGGGAAATGGGTCGGGTACGACCGCCAGAGTGACGTGGCCGGGTTATCGGGTTATTAATAACTCCACTGAGGCCAGTCAGTTCACGGTTGGGGCGTTTATACAAGGAAATGAGTGGCTCAATTCTACCGGAATCCCGTACTTTGCCGGTTTATCTTAA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0003674 GO:0003824 GO:0004857 GO:0005575 GO:0005623 GO:0006950 GO:0006952 GO:0008150 GO:0009605 GO:0009607 GO:0009617 GO:0016787 GO:0016788 GO:0030234 GO:0030599 GO:0042742 GO:0043086 GO:0043207 GO:0044092 GO:0044464 GO:0046910 GO:0050790 GO:0050829 GO:0050896 GO:0051704 GO:0051707 GO:0052689 GO:0065007 GO:0065009 GO:0071944 GO:0098542 GO:0098772

KEGG Pathways

Metabolic & Signaling

ko00040 ko01100 map00040 map01100

Pfam Domains

Protein Families

PMEI Pectinesterase

Protein Analysis

568

Amino Acids

62.5

Weight (kDa)

9.27

Isoelectric Point (pI)

27.27

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
PMEI	PF04043	56 - 209	6.2e-30	Plant invertase/pectin methylesterase inhibitor
Pectinesterase	PF01095	255 - 552	1.4e-145	Pectinesterase

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0014882)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT4G02300 AT4G02320
fragaria_vesca	FvH4_2g25980
malus_domestica	MD11G1307200.v1.1
prunus_persica	Prupe.8G264100_v2.0.a1
rosa_chinensis	RchiOBHm_Chr6g0294341
rosa_laevigata	RLG00000011879
rosa_multiflora	Rmu_co8392343.1_g000001 Rmu_sc0005369.1_g000009
rosa_roxburghii	Rroxscaffold_7G00173460
rosa_rugosa	Rorug06G0243600
rosa_samantha	Rh6AG356200 Rh6BG362900 Rh6CG370200 Rh6DG356700
rosa_wichuraiana	Rw6G031040

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AarI	CACCTGC	1 cut(s)	860
AasI	GACNNNNNNGTC	2 cut(s)	203, 401
Acc36I	ACCTGC	2 cut(s)	605, 860
AccB1I	GGYRCC	1 cut(s)	1202
AccBSI	CCGCTC	1 cut(s)	1095
AccI	GTMKAC	2 cut(s)	975, 1209
AccII	CGCG	5 cut(s)	407, 815, 1185, 1311, 1367
AclI	AACGTT	2 cut(s)	1046, 1394
AclWI	GGATC	2 cut(s)	705, 718
AcoI	YGGCCR	5 cut(s)	456, 999, 1279, 1411, 1583
AcsI	RAATTY	2 cut(s)	119, 913
AcuI	CTGAAG	2 cut(s)	36, 592
AcvI	CACGTG	1 cut(s)	516
AfaI	GTAC	8 cut(s)	287, 512, 550, 664, 878, 1430, 1564, 1685
AfiI	CCNNNNNNNGG	5 cut(s)	703, 720, 1277, 1381, 1485
AflIII	ACRYGT	2 cut(s)	513, 515
AgsI	TTSAA	3 cut(s)	589, 1399, 1543
AhlI	ACTAGT	2 cut(s)	542, 737
AjiI	CACGTC	1 cut(s)	1581
AjnI	CCWGG	2 cut(s)	845, 1302
Alw21I	GWGCWC	3 cut(s)	239, 390, 1520
Alw26I	GTCTC	2 cut(s)	211, 524
AlwI	GGATC	2 cut(s)	705, 718
Ama87I	CYCGRG	1 cut(s)	835
AoxI	GGCC	7 cut(s)	456, 999, 1279, 1306, 1411, 1583, 1617
ApeKI	GCWGC	5 cut(s)	361, 409, 484, 821, 1128
ApoI	RAATTY	2 cut(s)	119, 913
ArsI	GACNNNNNNTTYG	2 cut(s)	1202, 1234
AseI	ATTAAT	1 cut(s)	1602
Asp700I	GAANNNNTTC	1 cut(s)	1292
AspLEI	GCGC	4 cut(s)	409, 494, 619, 842
AspS9I	GGNCC	5 cut(s)	1064, 1306, 1315, 1372, 1474
AsuC2I	CCSGG	5 cut(s)	1264, 1435, 1483, 1547, 1587
AsuHPI	GGTGA	6 cut(s)	412, 790, 799, 941, 1288, 1351
AvaI	CYCGRG	1 cut(s)	835
AvaII	GGWCC	4 cut(s)	1064, 1315, 1372, 1474
AxyI	CCTNAGG	1 cut(s)	1448
BamHI	GGATCC	1 cut(s)	710
BanI	GGYRCC	1 cut(s)	1202
BanII	GRGCYC	2 cut(s)	239, 390
BbrPI	CACGTG	1 cut(s)	516
BbsI	GAAGAC	3 cut(s)	914, 950, 1231
Bbv12I	GWGCWC	3 cut(s)	239, 390, 1520
BbvI	GCAGC	5 cut(s)	373, 421, 471, 808, 1140
BccI	CCATC	4 cut(s)	134, 812, 926, 1349
BceAI	ACGGC	7 cut(s)	338, 994, 995, 1216, 1217, 1294, 1398
BcgI	CGANNNNNNTGC	4 cut(s)	1030, 1064, 1117, 1151
BciT130I	CCWGG	2 cut(s)	847, 1304
BcnI	CCSGG	5 cut(s)	1264, 1435, 1483, 1547, 1587
BcoDI	GTCTC	2 cut(s)	211, 524
BcuI	ACTAGT	2 cut(s)	542, 737
BfaI	CTAG	2 cut(s)	543, 738
BfoI	RGCGCY	1 cut(s)	495
BfuAI	ACCTGC	2 cut(s)	605, 860
BlpI	GCTNAGC	1 cut(s)	488
BmcAI	AGTACT	2 cut(s)	664, 1430
Bme1390I	CCNGG	7 cut(s)	847, 1264, 1304, 1435, 1483, 1547, 1587
Bme18I	GGWCC	4 cut(s)	1064, 1315, 1372, 1474
BmeT110I	CYCGRG	1 cut(s)	835
BmgBI	CACGTC	1 cut(s)	1581
BmgT120I	GGNCC	5 cut(s)	1064, 1306, 1315, 1372, 1474
BmiI	GGNNCC	8 cut(s)	679, 695, 712, 828, 1102, 1204, 1317, 1476
BmrFI	CCNGG	7 cut(s)	847, 1264, 1304, 1435, 1483, 1547, 1587
BmsI	GCATC	2 cut(s)	330, 615
BoxI	GACNNNNGTC	1 cut(s)	378
BpiI	GAAGAC	3 cut(s)	914, 950, 1231
BpmI	CTGGAG	2 cut(s)	596, 1511
Bpu1102I	GCTNAGC	1 cut(s)	488
BpuEI	CTTGAG	4 cut(s)	398, 404, 496, 614
BpuMI	CCSGG	5 cut(s)	1264, 1435, 1483, 1547, 1587
BsaAI	YACGTR	1 cut(s)	516
BsaJI	CCNNGG	4 cut(s)	1302, 1303, 1418, 1546
BsaWI	WCCGGW	3 cut(s)	1331, 1381, 1673
BsaXI	ACNNNNNCTCC	2 cut(s)	1148, 1178
Bsc4I	CCNNNNNNNGG	5 cut(s)	703, 720, 1277, 1381, 1485
Bse118I	RCCGGY	3 cut(s)	317, 451, 1691
Bse1I	ACTGG	4 cut(s)	508, 579, 1174, 1620
Bse21I	CCTNAGG	1 cut(s)	1448
Bse3DI	GCAATG	2 cut(s)	504, 619
BseBI	CCWGG	2 cut(s)	847, 1304
BseDI	CCNNGG	4 cut(s)	1302, 1303, 1418, 1546
BseGI	GGATG	2 cut(s)	126, 1341
BseLI	CCNNNNNNNGG	5 cut(s)	703, 720, 1277, 1381, 1485
BseMI	GCAATG	2 cut(s)	504, 619
BseMII	CTCAG	6 cut(s)	224, 448, 502, 1071, 1439, 1605
BseNI	ACTGG	4 cut(s)	508, 579, 1174, 1620
BseRI	GAGGAG	1 cut(s)	1035
BseX3I	CGGCCG	1 cut(s)	1411
BseXI	GCAGC	5 cut(s)	373, 421, 471, 808, 1140
BsgI	GTGCAG	1 cut(s)	298
Bsh1236I	CGCG	5 cut(s)	407, 815, 1185, 1311, 1367
Bsh1285I	CGRYCG	3 cut(s)	998, 1414, 1569
BshFI	GGCC	7 cut(s)	458, 1001, 1281, 1308, 1413, 1585, 1619
BshNI	GGYRCC	1 cut(s)	1202
BsiEI	CGRYCG	3 cut(s)	998, 1414, 1569
BsiHKAI	GWGCWC	3 cut(s)	239, 390, 1520
BsiHKCI	CYCGRG	1 cut(s)	835
BslFI	GGGAC	2 cut(s)	233, 1516
BslI	CCNNNNNNNGG	5 cut(s)	703, 720, 1277, 1381, 1485
BsmAI	GTCTC	2 cut(s)	211, 524
BsmBI	CGTCTC	1 cut(s)	211
BsmFI	GGGAC	2 cut(s)	233, 1516
BsmI	GAATGC	1 cut(s)	1193
BsnI	GGCC	7 cut(s)	458, 1001, 1281, 1308, 1413, 1585, 1619
BsoBI	CYCGRG	1 cut(s)	835
Bsp1286I	GDGCHC	3 cut(s)	239, 390, 1520
Bsp143I	GATC	3 cut(s)	710, 763, 995
Bsp1720I	GCTNAGC	1 cut(s)	488
BspANI	GGCC	7 cut(s)	458, 1001, 1281, 1308, 1413, 1585, 1619
BspCNI	CTCAG	6 cut(s)	225, 447, 501, 1070, 1440, 1606
BspFNI	CGCG	5 cut(s)	407, 815, 1185, 1311, 1367
BspLI	GGNNCC	8 cut(s)	679, 695, 712, 828, 1102, 1204, 1317, 1476
BspMI	ACCTGC	2 cut(s)	605, 860
BspPI	GGATC	2 cut(s)	705, 718
BspQI	GCTCTTC	1 cut(s)	395
BspT107I	GGYRCC	1 cut(s)	1202
BsrBI	CCGCTC	1 cut(s)	1095
BsrDI	GCAATG	2 cut(s)	504, 619
BsrFI	RCCGGY	3 cut(s)	317, 451, 1691
BsrI	ACTGG	4 cut(s)	508, 579, 1174, 1620
BssAI	RCCGGY	3 cut(s)	317, 451, 1691
BssECI	CCNNGG	4 cut(s)	1302, 1303, 1418, 1546
BssMI	GATC	3 cut(s)	710, 763, 995
Bst2UI	CCWGG	2 cut(s)	847, 1304
Bst6I	CTCTTC	1 cut(s)	395
BstBAI	YACGTR	1 cut(s)	516
BstC8I	GCNNGC	5 cut(s)	348, 366, 453, 602, 1073
BstDEI	CTNAG	7 cut(s)	11, 233, 434, 488, 1057, 1448, 1614
BstDSI	CCRYGG	1 cut(s)	1418
BstEII	GGTNACC	2 cut(s)	418, 1357
BstF5I	GGATG	2 cut(s)	126, 1341
BstFNI	CGCG	5 cut(s)	407, 815, 1185, 1311, 1367
BstH2I	RGCGCY	1 cut(s)	495
BstHHI	GCGC	4 cut(s)	409, 494, 619, 842
BstKTI	GATC	3 cut(s)	713, 766, 998
BstMAI	GTCTC	2 cut(s)	211, 524
BstMBI	GATC	3 cut(s)	710, 763, 995
BstMCI	CGRYCG	3 cut(s)	998, 1414, 1569
BstMWI	GCNNNNNNNGC	6 cut(s)	40, 566, 625, 821, 1007, 1137
BstNI	CCWGG	2 cut(s)	847, 1304
BstNSI	RCATGY	1 cut(s)	177
BstPAI	GACNNNNGTC	1 cut(s)	378
BstPI	GGTNACC	2 cut(s)	418, 1357
BstSCI	CCNGG	7 cut(s)	845, 1262, 1302, 1433, 1481, 1545, 1585
BstUI	CGCG	5 cut(s)	407, 815, 1185, 1311, 1367
BstV1I	GCAGC	5 cut(s)	373, 421, 471, 808, 1140
BstV2I	GAAGAC	3 cut(s)	914, 950, 1231
BstX2I	RGATCY	1 cut(s)	710
BstYI	RGATCY	1 cut(s)	710
BstZI	CGGCCG	1 cut(s)	1411
Bsu36I	CCTNAGG	1 cut(s)	1448
BsuRI	GGCC	7 cut(s)	458, 1001, 1281, 1308, 1413, 1585, 1619
BtgI	CCRYGG	1 cut(s)	1418
BtgZI	GCGATG	1 cut(s)	1015
BtrI	CACGTC	1 cut(s)	1581
BtsCI	GGATG	2 cut(s)	126, 1341
BtsIMutI	CAGTG	3 cut(s)	1002, 1467, 1611
BveI	ACCTGC	2 cut(s)	605, 860
Cac8I	GCNNGC	5 cut(s)	348, 366, 453, 602, 1073
CfoI	GCGC	4 cut(s)	409, 494, 619, 842
Cfr10I	RCCGGY	3 cut(s)	317, 451, 1691
Cfr13I	GGNCC	5 cut(s)	1064, 1306, 1315, 1372, 1474
CseI	GACGC	4 cut(s)	193, 490, 821, 1356
CsiI	ACCWGGT	1 cut(s)	845
Csp6I	GTAC	8 cut(s)	286, 511, 549, 663, 877, 1429, 1563, 1684
CviAII	CATG	6 cut(s)	131, 174, 283, 424, 682, 702
CviQI	GTAC	8 cut(s)	286, 511, 549, 663, 877, 1429, 1563, 1684
DdeI	CTNAG	7 cut(s)	11, 233, 434, 488, 1057, 1448, 1614
DpnI	GATC	3 cut(s)	712, 765, 997
DpnII	GATC	3 cut(s)	710, 763, 995
DrdI	GACNNNNNNGTC	2 cut(s)	203, 401
DseDI	GACNNNNNNGTC	2 cut(s)	203, 401
EaeI	YGGCCR	5 cut(s)	456, 999, 1279, 1411, 1583
EagI	CGGCCG	1 cut(s)	1411
Eam1104I	CTCTTC	1 cut(s)	395
EarI	CTCTTC	1 cut(s)	395
EciI	GGCGGA	2 cut(s)	1102, 1160
Ecl136II	GAGCTC	2 cut(s)	237, 388
EclXI	CGGCCG	1 cut(s)	1411
Eco24I	GRGCYC	2 cut(s)	239, 390
Eco47I	GGWCC	4 cut(s)	1064, 1315, 1372, 1474
Eco52I	CGGCCG	1 cut(s)	1411
Eco53kI	GAGCTC	2 cut(s)	237, 388
Eco57I	CTGAAG	2 cut(s)	36, 592
Eco72I	CACGTG	1 cut(s)	516
Eco81I	CCTNAGG	1 cut(s)	1448
Eco88I	CYCGRG	1 cut(s)	835
Eco91I	GGTNACC	2 cut(s)	418, 1357
EcoICRI	GAGCTC	2 cut(s)	237, 388
EcoO109I	RGGNCCY	1 cut(s)	1315
EcoO65I	GGTNACC	2 cut(s)	418, 1357
EcoRII	CCWGG	2 cut(s)	845, 1302
EcoT38I	GRGCYC	2 cut(s)	239, 390
Esp3I	CGTCTC	1 cut(s)	211
FaeI	CATG	6 cut(s)	134, 177, 286, 427, 685, 705
FalI	AAGNNNNNCTT	2 cut(s)	1277, 1309
FaqI	GGGAC	2 cut(s)	233, 1516
FatI	CATG	6 cut(s)	130, 173, 282, 423, 681, 701
FauI	CCCGC	2 cut(s)	258, 1486
FblI	GTMKAC	2 cut(s)	975, 1209
FokI	GGATG	2 cut(s)	113, 1328
FriOI	GRGCYC	2 cut(s)	239, 390
FspBI	CTAG	2 cut(s)	543, 738
GlaI	GCGC	4 cut(s)	408, 493, 618, 841
GsuI	CTGGAG	2 cut(s)	596, 1511
HaeII	RGCGCY	1 cut(s)	495
HaeIII	GGCC	7 cut(s)	458, 1001, 1281, 1308, 1413, 1585, 1619
HgaI	GACGC	4 cut(s)	193, 490, 821, 1356
HhaI	GCGC	4 cut(s)	409, 494, 619, 842
Hin1II	CATG	6 cut(s)	134, 177, 286, 427, 685, 705
Hin6I	GCGC	4 cut(s)	407, 492, 617, 840
HinP1I	GCGC	4 cut(s)	407, 492, 617, 840
HincII	GTYRAC	4 cut(s)	274, 976, 1210, 1392
HindII	GTYRAC	4 cut(s)	274, 976, 1210, 1392
HinfI	GANTC	5 cut(s)	229, 533, 637, 1335, 1677
HphI	GGTGA	6 cut(s)	412, 790, 799, 941, 1288, 1351
Hpy166II	GTNNAC	9 cut(s)	274, 513, 976, 1162, 1210, 1392, 1444, 1474, 1629
Hpy188I	TCNGA	9 cut(s)	234, 430, 677, 710, 723, 995, 1019, 1105, 1372
Hpy188III	TCNNGA	9 cut(s)	136, 208, 226, 371, 383, 521, 631, 1051, 1399
Hpy8I	GTNNAC	9 cut(s)	274, 513, 976, 1162, 1210, 1392, 1444, 1474, 1629
HpyAV	CCTTC	5 cut(s)	9, 518, 559, 1126, 1417
HpyCH4IV	ACGT	7 cut(s)	515, 1046, 1158, 1394, 1505, 1520, 1580
HpyCH4V	TGCA	7 cut(s)	315, 600, 733, 1128, 1251, 1353, 1511
HpyF10VI	GCNNNNNNNGC	6 cut(s)	40, 566, 625, 821, 1007, 1137
HpyF3I	CTNAG	7 cut(s)	11, 233, 434, 488, 1057, 1448, 1614
HpySE526I	ACGT	7 cut(s)	515, 1046, 1158, 1394, 1505, 1520, 1580
Hsp92II	CATG	6 cut(s)	134, 177, 286, 427, 685, 705
HspAI	GCGC	4 cut(s)	407, 492, 617, 840
KroI	GCCGGC	1 cut(s)	451
KroNI	GCCGGC	1 cut(s)	453
Kzo9I	GATC	3 cut(s)	710, 763, 995
LguI	GCTCTTC	1 cut(s)	395
LmnI	GCTCC	6 cut(s)	48, 443, 677, 832, 1100, 1106
Lsp1109I	GCAGC	5 cut(s)	373, 421, 471, 808, 1140
LweI	GCATC	2 cut(s)	330, 615
MabI	ACCWGGT	1 cut(s)	845
MaeI	CTAG	2 cut(s)	543, 738
MaeII	ACGT	7 cut(s)	515, 1046, 1158, 1394, 1505, 1520, 1580
MaeIII	GTNAC	3 cut(s)	418, 1357, 1576
MalI	GATC	3 cut(s)	712, 765, 997
MbiI	CCGCTC	1 cut(s)	1095
MboI	GATC	3 cut(s)	710, 763, 995
MboII	GAAGA	8 cut(s)	382, 646, 919, 955, 1210, 1231, 1285, 1297
MflI	RGATCY	1 cut(s)	710
MhlI	GDGCHC	3 cut(s)	239, 390, 1520
MluCI	AATT	4 cut(s)	119, 913, 936, 1666
MmeI	TCCRAC	7 cut(s)	327, 333, 408, 688, 997, 1128, 1350
MreI	CGCCGGCG	1 cut(s)	451
MroNI	GCCGGC	1 cut(s)	451
MroXI	GAANNNNTTC	1 cut(s)	1292
MseI	TTAA	4 cut(s)	555, 1347, 1602, 1702
MspR9I	CCNGG	7 cut(s)	847, 1264, 1304, 1435, 1483, 1547, 1587
Mva1269I	GAATGC	1 cut(s)	1193
MvaI	CCWGG	2 cut(s)	847, 1304
MvnI	CGCG	5 cut(s)	407, 815, 1185, 1311, 1367
MwoI	GCNNNNNNNGC	6 cut(s)	40, 566, 625, 821, 1007, 1137
NaeI	GCCGGC	1 cut(s)	453
NciI	CCSGG	5 cut(s)	1264, 1435, 1483, 1547, 1587
NdeII	GATC	3 cut(s)	710, 763, 995
NgoMIV	GCCGGC	1 cut(s)	451
NlaIII	CATG	6 cut(s)	134, 177, 286, 427, 685, 705
NlaIV	GGNNCC	8 cut(s)	679, 695, 712, 828, 1102, 1204, 1317, 1476
NmeAIII	GCCGAG	1 cut(s)	1389
NmuCI	GTSAC	3 cut(s)	418, 1357, 1576
NspI	RCATGY	1 cut(s)	177
PaeR7I	CTCGAG	1 cut(s)	835
PaqCI	CACCTGC	1 cut(s)	860
PasI	CCCWGGG	1 cut(s)	1303
PciSI	GCTCTTC	1 cut(s)	395
PcsI	WCGNNNNNNNCGW	4 cut(s)	399, 1206, 1233, 1637
PctI	GAATGC	1 cut(s)	1193
PdiI	GCCGGC	1 cut(s)	453
PdmI	GAANNNNTTC	1 cut(s)	1292
PfeI	GAWTC	5 cut(s)	229, 533, 637, 1335, 1677
PflFI	GACNNNGTC	1 cut(s)	401
PfoI	TCCNGGA	1 cut(s)	1433
Ple19I	CGATCG	1 cut(s)	998
PmaCI	CACGTG	1 cut(s)	516
PmlI	CACGTG	1 cut(s)	516
Ppu21I	YACGTR	1 cut(s)	516
PpuMI	RGGWCCY	1 cut(s)	1315
PshAI	GACNNNNGTC	1 cut(s)	378
PshBI	ATTAAT	1 cut(s)	1602
Psp124BI	GAGCTC	2 cut(s)	239, 390
Psp1406I	AACGTT	2 cut(s)	1046, 1394
Psp5II	RGGWCCY	1 cut(s)	1315
Psp6I	CCWGG	2 cut(s)	845, 1302
PspCI	CACGTG	1 cut(s)	516
PspEI	GGTNACC	2 cut(s)	418, 1357
PspGI	CCWGG	2 cut(s)	845, 1302
PspN4I	GGNNCC	8 cut(s)	679, 695, 712, 828, 1102, 1204, 1317, 1476
PspPI	GGNCC	5 cut(s)	1064, 1306, 1315, 1372, 1474
PspPPI	RGGWCCY	1 cut(s)	1315
PspXI	VCTCGAGB	1 cut(s)	835
PsuI	RGATCY	1 cut(s)	710
PsyI	GACNNNGTC	1 cut(s)	401
PvuI	CGATCG	1 cut(s)	998
RsaI	GTAC	8 cut(s)	287, 512, 550, 664, 878, 1430, 1564, 1685
RsaNI	GTAC	8 cut(s)	286, 511, 549, 663, 877, 1429, 1563, 1684
SacI	GAGCTC	2 cut(s)	239, 390
SalI	GTCGAC	2 cut(s)	974, 1208
SapI	GCTCTTC	1 cut(s)	395
SaqAI	TTAA	4 cut(s)	555, 1347, 1602, 1702
Sau3AI	GATC	3 cut(s)	710, 763, 995
Sau96I	GGNCC	5 cut(s)	1064, 1306, 1315, 1372, 1474
ScaI	AGTACT	2 cut(s)	664, 1430
ScrFI	CCNGG	7 cut(s)	847, 1264, 1304, 1435, 1483, 1547, 1587
SduI	GDGCHC	3 cut(s)	239, 390, 1520
SexAI	ACCWGGT	1 cut(s)	845
SfaNI	GCATC	2 cut(s)	330, 615
Sfr274I	CTCGAG	1 cut(s)	835
SgrAI	CRCCGGYG	1 cut(s)	451
SinI	GGWCC	4 cut(s)	1064, 1315, 1372, 1474
SlaI	CTCGAG	1 cut(s)	835
SmlI	CTYRAG	5 cut(s)	383, 413, 475, 629, 835
SmoI	CTYRAG	5 cut(s)	383, 413, 475, 629, 835
SpeI	ACTAGT	2 cut(s)	542, 737
Sse9I	AATT	4 cut(s)	119, 913, 936, 1666
SspI	AATATT	1 cut(s)	1538
SspMI	CTAG	2 cut(s)	543, 738
SstI	GAGCTC	2 cut(s)	239, 390
StyD4I	CCNGG	7 cut(s)	845, 1262, 1302, 1433, 1481, 1545, 1585
TaiI	ACGT	7 cut(s)	518, 1049, 1161, 1397, 1508, 1523, 1583
TasI	AATT	4 cut(s)	119, 913, 936, 1666
TatI	WGTACW	3 cut(s)	510, 662, 1428
TauI	GCSGC	7 cut(s)	353, 458, 497, 827, 1232, 1311, 1367
TfiI	GAWTC	5 cut(s)	229, 533, 637, 1335, 1677
Tru1I	TTAA	4 cut(s)	555, 1347, 1602, 1702
Tru9I	TTAA	4 cut(s)	555, 1347, 1602, 1702
TscAI	CASTG	3 cut(s)	1009, 1474, 1618
TseFI	GTSAC	3 cut(s)	418, 1357, 1576
TseI	GCWGC	5 cut(s)	361, 409, 484, 821, 1128
Tsp45I	GTSAC	3 cut(s)	418, 1357, 1576
TspDTI	ATGAA	3 cut(s)	698, 1018, 1204
TspGWI	ACGGA	1 cut(s)	1407
TspRI	CASTG	3 cut(s)	1009, 1474, 1618
Tth111I	GACNNNGTC	1 cut(s)	401
VpaK11BI	GGWCC	4 cut(s)	1064, 1315, 1372, 1474
VspI	ATTAAT	1 cut(s)	1602
XapI	RAATTY	2 cut(s)	119, 913
XceI	RCATGY	1 cut(s)	177
XhoI	CTCGAG	1 cut(s)	835
XmiI	GTMKAC	2 cut(s)	975, 1209
XmnI	GAANNNNTTC	1 cut(s)	1292
XspI	CTAG	2 cut(s)	543, 738
ZrmI	AGTACT	2 cut(s)	664, 1430

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.