Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr11

Orthogroup ID

OG0014882

Physical Location & Seq

Genomic Coordinates Reverse (-)

42133236 .. 42138138

Gene Structure 4903 bp

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UTR

Exon/CDS

Intron

Transcript / Protein ID

MD11G1307200.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 1740 bp

ATGTACAATCTAAGAAGAACAAAAATCTTGCTCTCAACATTGTTTCTAACTTCAATCCTTTTCCTAAGCCTACTCTTTTCAAAACCACATTTCAAAAACTCAAACTCAAAAAGCCCAAAAATCCATTTCCAAAACCACATTCAAAAACAGGTTCTAGAAACAGCCCATTGGGCATGCCAAGACACACTCTACAAATCCCTCTGCCTCTCCACCCTCGCCACCTTCCCCAATCTTGCCTCCAAAACCCTCCCGCAAATCATCTCCTCCACCGTCTCCCACACCGTGTTGGAAGTCAAAGCCTCCTCCTCCAACTGCACCAGCATCAAAAAGCAGTTCAGCAAAAAGCTCAGCTCGCTTCAGCAGTGGGCTCTCGACGACTGCCTTGAGCTCCTCGACAACACAATCGACGAGCTCAAGTCAGCCATCTCCGACCTCTCCACCAACAAAACACTCAACTCCAACCGCTACTACGACTTGAAAGCCCTACTCAGTGCTGCAATGACCAACCAGGACACGTGTCTCGATGGGTTTAGTGCTAACGGCACTGCTCCTCGGAGTAGCAATATGGGAATTGTGAGGAGCGAAATTGAAGGTAGAATAAATACAATCTCACAGCACGTTAGCAATGCGCTCGTTATGCTTAAGAAGATTCCGGGAGCTCAGAAGTACGAGTACTCTGAGACGATGAAGTCCGAGATGTTCCCCGACTTCGGACCTGCCAAAAAGAAGTTCCCGACTTGGGTTTCATCGAAAGATCGAAGGTTGTTGCAGTCCTCGACGAATGAGACAAAGTACGATTTGGTGGTGGCCAAGGACGGCACTGGAAACTTCACTACCATCAGCGAGGCCGTCGATGTGGCTCCTAATTCAAGCGCAACCAGGTTTGTGATATACATAAAGGCCGGGGCATATTTTGAGAATGTGGAGGTTGTGAAGAAGAAGACAAATCTGATGTTTTTGGGAGATGGGATTGGAAACACGATTGTCAAAGCCAGCCGGAATGTAGTGGATGGGTGGACTACTTTCCGATCAGCCACTGTCGCTGTGGTAGGAGACGGATTCATCGCCAAAGGAATCACATTCGAAAACGGAGCCGGCCCAAGTAAACACCAAGCAGTCGCCTTAAGAAGCGGCGCCGACTTTTCCGCATTCTACAAGTGCAGCTTCGTCGGCTACCAAGACACCCTCTACGTCCACTCCCTCCGCCAGTTCTACCGCGAATGCGACGTCTACGGCACCGTCGACTTTATCTTCGGCAACGCCGCCGTCGTCCTCCAGAACTGCAACCTCTACGCCCGCAAGCCGAACGAGAACCAGAAAAACATCTTCACCGCCCAGGGAAGAGAGGACCCGAACCAGAACACCGGGATTTCGATCCTGAATTGCAAAGTCACGGCCGGGTCGGATTTGATTCCGGTGAAATCGAATTTTACAACGTATCTGGGCCGTCCGTGGAAGGAGTATTCGAGGACCGTTTATCTGAAATCGTATATTGATGATTTGGTGGACCCCGCTGGATGGTTGGAGTGGAACGGGTCGTTTGCGTTGAGTACGTTGTATTATGGGGAGTATTTGAACCGTGGACCCGGTTCGAATACGAGCGCGAGGGTGACGTGGCCAGGTTATCGGGTCATTAATAGTTCGGTTGAGGCAAGTCAGTTCACTGTAGGAGAATTTATACAGGGAAGTGACTGGCTCAATTCTACCAATATTCCTTATTTTCTTGGTTTGACTAGTTGA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0003674 GO:0003824 GO:0004857 GO:0005575 GO:0005623 GO:0006950 GO:0006952 GO:0008150 GO:0009605 GO:0009607 GO:0009617 GO:0016787 GO:0016788 GO:0030234 GO:0030599 GO:0042742 GO:0043086 GO:0043207 GO:0044092 GO:0044464 GO:0046910 GO:0050790 GO:0050829 GO:0050896 GO:0051704 GO:0051707 GO:0052689 GO:0065007 GO:0065009 GO:0071944 GO:0098542 GO:0098772

KEGG Pathways

Metabolic & Signaling

ko00040 ko01100 map00040 map01100

Pfam Domains

Protein Families

PMEI Pectinesterase

Protein Analysis

580

Amino Acids

64.3

Weight (kDa)

9.04

Isoelectric Point (pI)

27.31

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
PMEI	PF04043	56 - 213	2.8e-29	Plant invertase/pectin methylesterase inhibitor
Pectinesterase	PF01095	266 - 562	1.1e-146	Pectinesterase

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0014882)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT4G02300 AT4G02320
fragaria_vesca	FvH4_2g25980
malus_domestica	MD11G1307200.v1.1
prunus_persica	Prupe.8G264100_v2.0.a1
rosa_chinensis	RchiOBHm_Chr6g0294341
rosa_laevigata	RLG00000011879
rosa_multiflora	Rmu_co8392343.1_g000001 Rmu_sc0005369.1_g000009
rosa_roxburghii	Rroxscaffold_7G00173460
rosa_rugosa	Rorug06G0243600
rosa_samantha	Rh6AG356200 Rh6BG362900 Rh6CG370200 Rh6DG356700
rosa_wichuraiana	Rw6G031040

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AasI	GACNNNNNNGTC	1 cut(s)	688
AatII	GACGTC	1 cut(s)	1230
Acc36I	ACCTGC	1 cut(s)	724
AccB1I	GGYRCC	2 cut(s)	1133, 1235
AccI	GTMKAC	2 cut(s)	1230, 1242
AccII	CGCG	2 cut(s)	1218, 1604
AclWI	GGATC	1 cut(s)	1369
AcoI	YGGCCR	3 cut(s)	807, 1395, 1616
AcsI	RAATTY	2 cut(s)	1426, 1673
AcuI	CTGAAG	1 cut(s)	341
AcvI	CACGTG	1 cut(s)	516
AcyI	GRCGYC	2 cut(s)	1134, 1227
AdeI	CACNNNGTG	1 cut(s)	283
AfaI	GTAC	5 cut(s)	5, 668, 674, 794, 1552
AfiI	CCNNNNNNNGG	3 cut(s)	710, 739, 1518
AflII	CTTAAG	2 cut(s)	641, 1123
AflIII	ACRYGT	2 cut(s)	513, 515
AgsI	TTSAA	8 cut(s)	54, 81, 94, 143, 478, 590, 870, 1576
AhlI	ACTAGT	1 cut(s)	1733
AjiI	CACGTC	1 cut(s)	1614
AjnI	CCWGG	4 cut(s)	507, 878, 1335, 1618
AjuI	GAANNNNNNNTTGG	4 cut(s)	123, 155, 713, 745
AluBI	AGCT	6 cut(s)	346, 351, 388, 412, 659, 1164
AluI	AGCT	6 cut(s)	346, 351, 388, 412, 659, 1164
Alw21I	GWGCWC	3 cut(s)	390, 414, 661
Alw26I	GTCTC	5 cut(s)	277, 524, 674, 779, 1047
AlwI	GGATC	1 cut(s)	1369
AlwNI	CAGNNNCTG	1 cut(s)	1037
AoxI	GGCC	7 cut(s)	807, 846, 900, 1096, 1395, 1444, 1616
ApeKI	GCWGC	2 cut(s)	494, 1161
ApoI	RAATTY	2 cut(s)	1426, 1673
ArsI	GACNNNNNNTTYG	4 cut(s)	1212, 1235, 1244, 1267
AseI	ATTAAT	1 cut(s)	1635
AspLEI	GCGC	4 cut(s)	631, 875, 1136, 1604
AspS9I	GGNCC	7 cut(s)	713, 1097, 1348, 1444, 1470, 1507, 1583
AsuC2I	CCSGG	5 cut(s)	654, 904, 1366, 1399, 1587
AsuHPI	GGTGA	3 cut(s)	1321, 1429, 1621
AsuII	TTCGAA	2 cut(s)	1083, 1592
AvaII	GGWCC	5 cut(s)	713, 1348, 1470, 1507, 1583
BalI	TGGCCA	2 cut(s)	809, 1618
BanI	GGYRCC	2 cut(s)	1133, 1235
BanII	GRGCYC	4 cut(s)	370, 390, 414, 661
BarI	GAAGNNNNNNTAC	2 cut(s)	656, 688
BbrPI	CACGTG	1 cut(s)	516
BbsI	GAAGAC	1 cut(s)	947
Bbv12I	GWGCWC	3 cut(s)	390, 414, 661
BbvI	GCAGC	2 cut(s)	481, 1173
BccI	CCATC	6 cut(s)	431, 518, 845, 959, 1004, 1512
BceAI	ACGGC	7 cut(s)	556, 832, 833, 1249, 1250, 1410, 1431
BcgI	CGANNNNNNTGC	4 cut(s)	613, 647, 1150, 1184
BciT130I	CCWGG	4 cut(s)	509, 880, 1337, 1620
BcnI	CCSGG	5 cut(s)	654, 904, 1366, 1399, 1587
BcoDI	GTCTC	5 cut(s)	277, 524, 674, 779, 1047
BcuI	ACTAGT	1 cut(s)	1733
BfaI	CTAG	2 cut(s)	155, 1734
BfmI	CTRYAG	1 cut(s)	1665
BfoI	RGCGCY	1 cut(s)	1137
BfrI	CTTAAG	2 cut(s)	641, 1123
BfuAI	ACCTGC	1 cut(s)	724
BglI	GCCNNNNNGGC	1 cut(s)	170
BisI	GCNGC	4 cut(s)	495, 1132, 1162, 1263
BlpI	GCTNAGC	1 cut(s)	347
BlsI	GCNGC	4 cut(s)	496, 1133, 1163, 1264
BmcAI	AGTACT	1 cut(s)	674
Bme1390I	CCNGG	9 cut(s)	509, 654, 880, 904, 1337, 1366, 1399, 1587, 1620
Bme18I	GGWCC	5 cut(s)	713, 1348, 1470, 1507, 1583
BmgBI	CACGTC	1 cut(s)	1614
BmgT120I	GGNCC	7 cut(s)	713, 1097, 1348, 1444, 1470, 1507, 1583
BmiI	GGNNCC	7 cut(s)	861, 1093, 1135, 1237, 1350, 1509, 1585
BmrFI	CCNGG	9 cut(s)	509, 654, 880, 904, 1337, 1366, 1399, 1587, 1620
BmsI	GCATC	1 cut(s)	330
BoxI	GACNNNNGTC	1 cut(s)	516
BpiI	GAAGAC	1 cut(s)	947
BpmI	CTGGAG	1 cut(s)	1259
Bpu10I	CCTNAGC	1 cut(s)	65
Bpu1102I	GCTNAGC	1 cut(s)	347
Bpu14I	TTCGAA	2 cut(s)	1083, 1592
BpuEI	CTTGAG	2 cut(s)	398, 404
BpuMI	CCSGG	5 cut(s)	654, 904, 1366, 1399, 1587
BsaAI	YACGTR	1 cut(s)	516
BsaBI	GATNNNNATC	1 cut(s)	1373
BsaHI	GRCGYC	2 cut(s)	1134, 1227
BsaJI	CCNNGG	7 cut(s)	551, 810, 903, 1335, 1336, 1451, 1579
BsaWI	WCCGGW	1 cut(s)	1414
BsaXI	ACNNNNNCTCC	2 cut(s)	1181, 1211
Bsc4I	CCNNNNNNNGG	3 cut(s)	710, 739, 1518
Bse118I	RCCGGY	1 cut(s)	1094
Bse1I	ACTGG	3 cut(s)	826, 1207, 1697
Bse3DI	GCAATG	2 cut(s)	504, 631
Bse8I	GATNNNNATC	1 cut(s)	1373
BseBI	CCWGG	4 cut(s)	509, 880, 1337, 1620
BseDI	CCNNGG	7 cut(s)	551, 810, 903, 1335, 1336, 1451, 1579
BseGI	GGATG	2 cut(s)	1015, 1523
BseJI	GATNNNNATC	1 cut(s)	1373
BseLI	CCNNNNNNNGG	3 cut(s)	710, 739, 1518
BseMI	GCAATG	2 cut(s)	504, 631
BseMII	CTCAG	4 cut(s)	361, 502, 669, 674
BseNI	ACTGG	3 cut(s)	826, 1207, 1697
BseRI	GAGGAG	6 cut(s)	253, 292, 295, 380, 540, 592
BseX3I	CGGCCG	1 cut(s)	1395
BseXI	GCAGC	2 cut(s)	481, 1173
BsgI	GTGCAG	2 cut(s)	298, 1180
Bsh1236I	CGCG	2 cut(s)	1218, 1604
Bsh1285I	CGRYCG	1 cut(s)	1398
BshFI	GGCC	7 cut(s)	809, 848, 902, 1098, 1397, 1446, 1618
BshNI	GGYRCC	2 cut(s)	1133, 1235
BsiEI	CGRYCG	1 cut(s)	1398
BsiHKAI	GWGCWC	3 cut(s)	390, 414, 661
BsiSI	CCGG	8 cut(s)	653, 903, 997, 1095, 1365, 1398, 1415, 1587
BslI	CCNNNNNNNGG	3 cut(s)	710, 739, 1518
BsmAI	GTCTC	5 cut(s)	277, 524, 674, 779, 1047
BsmBI	CGTCTC	3 cut(s)	277, 674, 1047
BsmI	GAATGC	2 cut(s)	1148, 1226
BsnI	GGCC	7 cut(s)	809, 848, 902, 1098, 1397, 1446, 1618
Bsp119I	TTCGAA	2 cut(s)	1083, 1592
Bsp1286I	GDGCHC	4 cut(s)	370, 390, 414, 661
Bsp1407I	TGTACA	1 cut(s)	3
Bsp143I	GATC	3 cut(s)	754, 1028, 1374
Bsp1720I	GCTNAGC	1 cut(s)	347
BspANI	GGCC	7 cut(s)	809, 848, 902, 1098, 1397, 1446, 1618
BspCNI	CTCAG	4 cut(s)	360, 501, 670, 673
BspFNI	CGCG	2 cut(s)	1218, 1604
BspLI	GGNNCC	7 cut(s)	861, 1093, 1135, 1237, 1350, 1509, 1585
BspMI	ACCTGC	1 cut(s)	724
BspPI	GGATC	1 cut(s)	1369
BspT104I	TTCGAA	2 cut(s)	1083, 1592
BspT107I	GGYRCC	2 cut(s)	1133, 1235
BspTI	CTTAAG	2 cut(s)	641, 1123
BsrDI	GCAATG	2 cut(s)	504, 631
BsrFI	RCCGGY	1 cut(s)	1094
BsrGI	TGTACA	1 cut(s)	3
BsrI	ACTGG	3 cut(s)	826, 1207, 1697
BssAI	RCCGGY	1 cut(s)	1094
BssECI	CCNNGG	7 cut(s)	551, 810, 903, 1335, 1336, 1451, 1579
BssMI	GATC	3 cut(s)	754, 1028, 1374
BssNI	GRCGYC	2 cut(s)	1134, 1227
BssT1I	CCWWGG	1 cut(s)	810
Bst2UI	CCWGG	4 cut(s)	509, 880, 1337, 1620
Bst4CI	ACNGT	7 cut(s)	271, 283, 1039, 1240, 1474, 1580, 1666
Bst6I	CTCTTC	1 cut(s)	1336
BstACI	GRCGYC	2 cut(s)	1134, 1227
BstAFI	CTTAAG	2 cut(s)	641, 1123
BstAUI	TGTACA	1 cut(s)	3
BstBAI	YACGTR	1 cut(s)	516
BstBI	TTCGAA	2 cut(s)	1083, 1592
BstC8I	GCNNGC	6 cut(s)	175, 353, 994, 1096, 1297, 1301
BstDEI	CTNAG	6 cut(s)	11, 65, 347, 488, 660, 678
BstDSI	CCRYGG	2 cut(s)	1451, 1579
BstF5I	GGATG	2 cut(s)	1015, 1523
BstFNI	CGCG	2 cut(s)	1218, 1604
BstH2I	RGCGCY	1 cut(s)	1137
BstHHI	GCGC	4 cut(s)	631, 875, 1136, 1604
BstKTI	GATC	3 cut(s)	757, 1031, 1377
BstMAI	GTCTC	5 cut(s)	277, 524, 674, 779, 1047
BstMBI	GATC	3 cut(s)	754, 1028, 1374
BstMCI	CGRYCG	1 cut(s)	1398
BstMWI	GCNNNNNNNGC	5 cut(s)	170, 352, 637, 1040, 1170
BstNI	CCWGG	4 cut(s)	509, 880, 1337, 1620
BstNSI	RCATGY	1 cut(s)	177
BstPAI	GACNNNNGTC	1 cut(s)	516
BstSCI	CCNGG	9 cut(s)	507, 652, 878, 902, 1335, 1364, 1397, 1585, 1618
BstSFI	CTRYAG	1 cut(s)	1665
BstUI	CGCG	2 cut(s)	1218, 1604
BstV1I	GCAGC	2 cut(s)	481, 1173
BstV2I	GAAGAC	1 cut(s)	947
BstZI	CGGCCG	1 cut(s)	1395
BsuRI	GGCC	7 cut(s)	809, 848, 902, 1098, 1397, 1446, 1618
BtgI	CCRYGG	2 cut(s)	1451, 1579
BtgZI	GCGATG	1 cut(s)	1048
BtrI	CACGTC	1 cut(s)	1614
BtsCI	GGATG	2 cut(s)	1015, 1523
BtsI	GCAGTG	2 cut(s)	368, 543
BtsIMutI	CAGTG	6 cut(s)	368, 496, 543, 819, 1035, 1662
BveI	ACCTGC	1 cut(s)	724
Cac8I	GCNNGC	6 cut(s)	175, 353, 994, 1096, 1297, 1301
CaiI	CAGNNNCTG	1 cut(s)	1037
CfoI	GCGC	4 cut(s)	631, 875, 1136, 1604
Cfr10I	RCCGGY	1 cut(s)	1094
Cfr13I	GGNCC	7 cut(s)	713, 1097, 1348, 1444, 1470, 1507, 1583
CsiI	ACCWGGT	1 cut(s)	878
Csp6I	GTAC	5 cut(s)	4, 667, 673, 793, 1551
CviAII	CATG	1 cut(s)	174
CviQI	GTAC	5 cut(s)	4, 667, 673, 793, 1551
DdeI	CTNAG	6 cut(s)	11, 65, 347, 488, 660, 678
DinI	GGCGCC	1 cut(s)	1135
DpnI	GATC	3 cut(s)	756, 1030, 1376
DpnII	GATC	3 cut(s)	754, 1028, 1374
DraIII	CACNNNGTG	1 cut(s)	283
DrdI	GACNNNNNNGTC	1 cut(s)	688
DseDI	GACNNNNNNGTC	1 cut(s)	688
EaeI	YGGCCR	3 cut(s)	807, 1395, 1616
EagI	CGGCCG	1 cut(s)	1395
Eam1104I	CTCTTC	1 cut(s)	1336
EarI	CTCTTC	1 cut(s)	1336
EciI	GGCGGA	1 cut(s)	1193
Ecl136II	GAGCTC	3 cut(s)	388, 412, 659
EclXI	CGGCCG	1 cut(s)	1395
Eco130I	CCWWGG	1 cut(s)	810
Eco24I	GRGCYC	4 cut(s)	370, 390, 414, 661
Eco47I	GGWCC	5 cut(s)	713, 1348, 1470, 1507, 1583
Eco52I	CGGCCG	1 cut(s)	1395
Eco53kI	GAGCTC	3 cut(s)	388, 412, 659
Eco57I	CTGAAG	1 cut(s)	341
Eco72I	CACGTG	1 cut(s)	516
EcoICRI	GAGCTC	3 cut(s)	388, 412, 659
EcoO109I	RGGNCCY	1 cut(s)	1348
EcoRII	CCWGG	4 cut(s)	507, 878, 1335, 1618
EcoT14I	CCWWGG	1 cut(s)	810
EcoT38I	GRGCYC	4 cut(s)	370, 390, 414, 661
EgeI	GGCGCC	1 cut(s)	1135
EheI	GGCGCC	1 cut(s)	1135
ErhI	CCWWGG	1 cut(s)	810
Esp3I	CGTCTC	3 cut(s)	277, 674, 1047
FaeI	CATG	1 cut(s)	177
FaiI	YATR	9 cut(s)	175, 566, 638, 892, 896, 910, 1491, 1563, 1679
FalI	AAGNNNNNCTT	2 cut(s)	1148, 1180
FatI	CATG	1 cut(s)	173
FauI	CCCGC	3 cut(s)	258, 1304, 1519
FblI	GTMKAC	2 cut(s)	1230, 1242
Fnu4HI	GCNGC	4 cut(s)	495, 1132, 1162, 1263
FokI	GGATG	2 cut(s)	1022, 1530
FriOI	GRGCYC	4 cut(s)	370, 390, 414, 661
Fsp4HI	GCNGC	4 cut(s)	495, 1132, 1162, 1263
FspBI	CTAG	2 cut(s)	155, 1734
GlaI	GCGC	4 cut(s)	630, 874, 1135, 1603
GluI	GCNGC	4 cut(s)	495, 1132, 1162, 1263
GsuI	CTGGAG	1 cut(s)	1259
HaeII	RGCGCY	1 cut(s)	1137
HaeIII	GGCC	7 cut(s)	809, 848, 902, 1098, 1397, 1446, 1618
HapII	CCGG	8 cut(s)	653, 903, 997, 1095, 1365, 1398, 1415, 1587
HhaI	GCGC	4 cut(s)	631, 875, 1136, 1604
Hin1I	GRCGYC	2 cut(s)	1134, 1227
Hin1II	CATG	1 cut(s)	177
Hin6I	GCGC	4 cut(s)	629, 873, 1134, 1602
HinP1I	GCGC	4 cut(s)	629, 873, 1134, 1602
HincII	GTYRAC	1 cut(s)	1243
HindII	GTYRAC	1 cut(s)	1243
HinfI	GANTC	4 cut(s)	649, 1059, 1074, 1411
HpaII	CCGG	8 cut(s)	653, 903, 997, 1095, 1365, 1398, 1415, 1587
HphI	GGTGA	3 cut(s)	1321, 1429, 1621
Hpy166II	GTNNAC	8 cut(s)	1017, 1106, 1195, 1231, 1243, 1507, 1583, 1662
Hpy188III	TCNNGA	6 cut(s)	155, 371, 521, 733, 1276, 1378
Hpy8I	GTNNAC	8 cut(s)	1017, 1106, 1195, 1231, 1243, 1507, 1583, 1662
Hpy99I	CGWCG	8 cut(s)	377, 410, 781, 854, 1172, 1229, 1244, 1271
HpyAV	CCTTC	4 cut(s)	232, 584, 753, 1450
HpyCH4III	ACNGT	7 cut(s)	271, 283, 1039, 1240, 1474, 1580, 1666
HpyCH4IV	ACGT	7 cut(s)	515, 618, 1191, 1227, 1436, 1553, 1613
HpyCH4V	TGCA	6 cut(s)	315, 497, 769, 1161, 1284, 1386
HpyF10VI	GCNNNNNNNGC	5 cut(s)	170, 352, 637, 1040, 1170
HpyF3I	CTNAG	6 cut(s)	11, 65, 347, 488, 660, 678
HpySE526I	ACGT	7 cut(s)	515, 618, 1191, 1227, 1436, 1553, 1613
Hsp92I	GRCGYC	2 cut(s)	1134, 1227
Hsp92II	CATG	1 cut(s)	177
HspAI	GCGC	4 cut(s)	629, 873, 1134, 1602
KasI	GGCGCC	1 cut(s)	1133
KroI	GCCGGC	1 cut(s)	1094
KroNI	GCCGGC	1 cut(s)	1096
Kzo9I	GATC	3 cut(s)	754, 1028, 1374
LmnI	GCTCC	6 cut(s)	393, 553, 579, 656, 865, 1091
Lsp1109I	GCAGC	2 cut(s)	481, 1173
LweI	GCATC	1 cut(s)	330
MabI	ACCWGGT	1 cut(s)	878
MaeI	CTAG	2 cut(s)	155, 1734
MaeII	ACGT	7 cut(s)	515, 618, 1191, 1227, 1436, 1553, 1613
MaeIII	GTNAC	3 cut(s)	1390, 1609, 1688
MalI	GATC	3 cut(s)	756, 1030, 1376
MboI	GATC	3 cut(s)	754, 1028, 1374
MboII	GAAGA	8 cut(s)	27, 658, 946, 949, 952, 1243, 1318, 1353
MhlI	GDGCHC	4 cut(s)	370, 390, 414, 661
MlsI	TGGCCA	2 cut(s)	809, 1618
MluCI	AATT	7 cut(s)	570, 585, 865, 1381, 1426, 1673, 1699
MluNI	TGGCCA	2 cut(s)	809, 1618
Mly113I	GGCGCC	1 cut(s)	1134
MmeI	TCCRAC	6 cut(s)	267, 333, 453, 483, 1383, 1503
Mox20I	TGGCCA	2 cut(s)	809, 1618
MroNI	GCCGGC	1 cut(s)	1094
MscI	TGGCCA	2 cut(s)	809, 1618
MseI	TTAA	3 cut(s)	642, 1124, 1635
Msp20I	TGGCCA	2 cut(s)	809, 1618
MspA1I	CMGCKG	1 cut(s)	1514
MspCI	CTTAAG	2 cut(s)	641, 1123
MspI	CCGG	8 cut(s)	653, 903, 997, 1095, 1365, 1398, 1415, 1587
MspR9I	CCNGG	9 cut(s)	509, 654, 880, 904, 1337, 1366, 1399, 1587, 1620
Mva1269I	GAATGC	2 cut(s)	1148, 1226
MvaI	CCWGG	4 cut(s)	509, 880, 1337, 1620
MvnI	CGCG	2 cut(s)	1218, 1604
MwoI	GCNNNNNNNGC	5 cut(s)	170, 352, 637, 1040, 1170
NaeI	GCCGGC	1 cut(s)	1096
NarI	GGCGCC	1 cut(s)	1134
NciI	CCSGG	5 cut(s)	654, 904, 1366, 1399, 1587
NdeII	GATC	3 cut(s)	754, 1028, 1374
NgoMIV	GCCGGC	1 cut(s)	1094
NlaIII	CATG	1 cut(s)	177
NlaIV	GGNNCC	7 cut(s)	861, 1093, 1135, 1237, 1350, 1509, 1585
NmuCI	GTSAC	3 cut(s)	1390, 1609, 1688
NspI	RCATGY	1 cut(s)	177
NspV	TTCGAA	2 cut(s)	1083, 1592
PaeI	GCATGC	1 cut(s)	177
PasI	CCCWGGG	1 cut(s)	1336
PcsI	WCGNNNNNNNCGW	2 cut(s)	1239, 1266
PctI	GAATGC	2 cut(s)	1148, 1226
PdiI	GCCGGC	1 cut(s)	1096
PfeI	GAWTC	4 cut(s)	649, 1059, 1074, 1411
PfoI	TCCNGGA	1 cut(s)	652
PkrI	GCNGC	4 cut(s)	496, 1133, 1163, 1264
PluTI	GGCGCC	1 cut(s)	1137
PmaCI	CACGTG	1 cut(s)	516
PmlI	CACGTG	1 cut(s)	516
Ppu21I	YACGTR	1 cut(s)	516
PpuMI	RGGWCCY	1 cut(s)	1348
PshAI	GACNNNNGTC	1 cut(s)	516
PshBI	ATTAAT	1 cut(s)	1635
Psp124BI	GAGCTC	3 cut(s)	390, 414, 661
Psp5II	RGGWCCY	1 cut(s)	1348
Psp6I	CCWGG	4 cut(s)	507, 878, 1335, 1618
PspCI	CACGTG	1 cut(s)	516
PspGI	CCWGG	4 cut(s)	507, 878, 1335, 1618
PspN4I	GGNNCC	7 cut(s)	861, 1093, 1135, 1237, 1350, 1509, 1585
PspPI	GGNCC	7 cut(s)	713, 1097, 1348, 1444, 1470, 1507, 1583
PspPPI	RGGWCCY	1 cut(s)	1348
PstNI	CAGNNNCTG	1 cut(s)	1037
RsaI	GTAC	5 cut(s)	5, 668, 674, 794, 1552
RsaNI	GTAC	5 cut(s)	4, 667, 673, 793, 1551
SacI	GAGCTC	3 cut(s)	390, 414, 661
SalI	GTCGAC	1 cut(s)	1241
SaqAI	TTAA	3 cut(s)	642, 1124, 1635
SatI	GCNGC	4 cut(s)	495, 1132, 1162, 1263
Sau3AI	GATC	3 cut(s)	754, 1028, 1374
Sau96I	GGNCC	7 cut(s)	713, 1097, 1348, 1444, 1470, 1507, 1583
ScaI	AGTACT	1 cut(s)	674
ScrFI	CCNGG	9 cut(s)	509, 654, 880, 904, 1337, 1366, 1399, 1587, 1620
SduI	GDGCHC	4 cut(s)	370, 390, 414, 661
SexAI	ACCWGGT	1 cut(s)	878
SfaNI	GCATC	1 cut(s)	330
SfcI	CTRYAG	1 cut(s)	1665
SfoI	GGCGCC	1 cut(s)	1135
SfuI	TTCGAA	2 cut(s)	1083, 1592
SinI	GGWCC	5 cut(s)	713, 1348, 1470, 1507, 1583
SmlI	CTYRAG	4 cut(s)	383, 413, 641, 1123
SmoI	CTYRAG	4 cut(s)	383, 413, 641, 1123
SpeI	ACTAGT	1 cut(s)	1733
SphI	GCATGC	1 cut(s)	177
Sse9I	AATT	7 cut(s)	570, 585, 865, 1381, 1426, 1673, 1699
SspDI	GGCGCC	1 cut(s)	1133
SspI	AATATT	1 cut(s)	1711
SspMI	CTAG	2 cut(s)	155, 1734
SstI	GAGCTC	3 cut(s)	390, 414, 661
StyD4I	CCNGG	9 cut(s)	507, 652, 878, 902, 1335, 1364, 1397, 1585, 1618
StyI	CCWWGG	1 cut(s)	810
TaaI	ACNGT	7 cut(s)	271, 283, 1039, 1240, 1474, 1580, 1666
TaiI	ACGT	7 cut(s)	518, 621, 1194, 1230, 1439, 1556, 1616
TasI	AATT	7 cut(s)	570, 585, 865, 1381, 1426, 1673, 1699
TatI	WGTACW	2 cut(s)	3, 672
TauI	GCSGC	2 cut(s)	1134, 1265
TfiI	GAWTC	4 cut(s)	649, 1059, 1074, 1411
Tru1I	TTAA	3 cut(s)	642, 1124, 1635
Tru9I	TTAA	3 cut(s)	642, 1124, 1635
TscAI	CASTG	6 cut(s)	368, 496, 550, 826, 1042, 1669
TseFI	GTSAC	3 cut(s)	1390, 1609, 1688
TseI	GCWGC	2 cut(s)	494, 1161
Tsp45I	GTSAC	3 cut(s)	1390, 1609, 1688
TspDTI	ATGAA	3 cut(s)	701, 735, 1051
TspGWI	ACGGA	3 cut(s)	1071, 1104, 1440
TspRI	CASTG	6 cut(s)	368, 496, 550, 826, 1042, 1669
Vha464I	CTTAAG	2 cut(s)	641, 1123
VpaK11BI	GGWCC	5 cut(s)	713, 1348, 1470, 1507, 1583
VspI	ATTAAT	1 cut(s)	1635
XapI	RAATTY	2 cut(s)	1426, 1673
XbaI	TCTAGA	1 cut(s)	154
XceI	RCATGY	1 cut(s)	177
XcmI	CCANNNNNNNNNTGG	1 cut(s)	1042
XmiI	GTMKAC	2 cut(s)	1230, 1242
XspI	CTAG	2 cut(s)	155, 1734
ZraI	GACGTC	1 cut(s)	1228
ZrmI	AGTACT	1 cut(s)	674

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.