Rw6G031040

pectinesterase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_wichuraiana
Chr6
OG0014882
Physical Location & Seq
Forward (+)
55114185 .. 55121354
7170 bp
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UTR
Exon/CDS
Intron
Rw6G031040.1

Sequence Viewer

Length: 1689 bp
ATGTATAGTCTAAGAAGGAGAACAAAGCTCTTGCTATCATTGCTTCCAACTTCAGCCTTCATCCTCATCCTATTCTTTTCACAAAGCCACTTCACAAATAGCCCCAAACCCACAAAAGAATTTATCATCCATGTCCAGAAGCACAACCAAGTAGCCCACTCAACATGCCAAAACACACTCTACAAAGATCTCTGCGTCTCGACCCTGACCTCATTCCCGAACCTGAGCTCGAAATCAGTCCCGGAAATCATATCCTCCACCGTCAACAAGACCATGTACGAGGTCATAGCCTCGAACTCCAACTGCACCAGCATCAAAAAGCACACCAAGACCAAGCTCGCCGCCGGTCAGCAGCAAGCTCTCGACGACTGTCTCGAGCTCTTCGACAACACCATTGCCGAGCTCAAGGCTGTCATGTCAGAACTCAACTCCAAGAAGTCGCCGGCGGCCAAGCACTACAACCACTTGAGGACGCTGCTCAGCGCCGCAATGACGAACCAGTACACGTGTCTCGAAGGGTTTGATTCCAGCAGTAGTACGGCGAAAGCTGTGAAGGATGAATTGGAAAGCAGATTGAATACTACCTGCAAGCACGTTAGCAATGCCCTTGTGATGCTCAAGAAGATTCCGGGAGTGGTTGAACGTGAGACTGACGAGTACGGACCCATGAAAAACGGGTTCCCTACATGGGTCGGATCCAAAGCTCGAAAACTGTTGCAGACTAGTTCGGCAAATCAGACTCAGTATGATCTGGTGGTGGCGAAAGACGGTAGCGGAAACTTTACCACCATCACCGACGCAGTGGCTGCGGCTCCAAATTCGAGCGCAACCAGGTTTGTCATATACATAAAAGCTGGTGCGTACTTTGAGAATGTAGAGGTTGTGAGCAAGAAGAAGAATTTGATGTTTTTGGGAGATGGGATTGGAAAGACGGTTGTGAAAGCGAGTAGGAACGTTGTCGACGGCTGGACTACTTTCCGATCGGCCACCGTCGCCGTGGTCGGAGGAGGGTTCATAGCCAAAGGCATAACCTTCGAGAACTCAGCCGGACCAAGCAAGCACCAAGCCGTAGCCCTCCGCTCCGGCTCGGACCTCTCCGTCTTCTACCAATGCAGCTTCGTCGGCTACCAAGACACTCTCTACGTCCACTCCCTCCGCCAGTTCTACGGCCAATGCGACGTCTACGGCACCGTCGACTTCATCTTCGGCAACGCAGCCGTCGTCTTCCAGAACTGCAACCTCTACGCTCGAAAGCCCAACGAGAACCAGAAGAACATCTTCACCGCCCAGGGCCGCGAGGACCCCAACCAGAACACCGGAATCTCCATCCTCAACTGCAAGGTCACCGCCGCGTCCGACCTCATTCCGGTCAAGTCCACGTTCAAGACGTATCTGGGCCGTCCGTGGAAGGAGTACTCCCGGACCGTTTATCTCAGGTCGAGTATCGACGACGTGGTGGACCCTGCGGGCTGGTTGGAGTGGAATGGCACGTTTGCGCTGAGCACGTTGTATTATGGAGAGTATTTGAACCGGGGAAACGGGTCGGATACGAGCGCCAGGGTGACGTGGCCGGGTTATCGGGTTATTAATAGCTCCACGGAGGCGAGTCAGTTCACGGTTGGGGCGTTTATACAGGGAAATGAGTGGCTCAATTCTACCGGAGTTCCGTACTTTGCTGGTTTAACATAA

Protein Analysis

562

Amino Acids

61.85

Weight (kDa)

9.13

Isoelectric Point (pI)

28.08

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
PMEI PF04043 53 - 205 2.9e-30 Plant invertase/pectin methylesterase inhibitor
Pectinesterase PF01095 250 - 547 7.1e-146 Pectinesterase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Orthologous Genes (Group: OG0014882)

Species Orthologous Gene IDs
arabidopsis_thaliana AT4G02300 AT4G02320
fragaria_vesca FvH4_2g25980
malus_domestica MD11G1307200.v1.1
prunus_persica Prupe.8G264100_v2.0.a1
rosa_chinensis RchiOBHm_Chr6g0294341
rosa_laevigata RLG00000011879
rosa_multiflora Rmu_co8392343.1_g000001 Rmu_sc0005369.1_g000009
rosa_roxburghii Rroxscaffold_7G00173460
rosa_rugosa Rorug06G0243600
rosa_samantha Rh6AG356200 Rh6BG362900 Rh6CG370200 Rh6DG356700
rosa_wichuraiana Rw6G031040

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 1097
AatII GACGTC 1 cut(s) 1182
Acc36I ACCTGC 1 cut(s) 593
AccB1I GGYRCC 1 cut(s) 1187
AccBSI CCGCTC 1 cut(s) 1080
AccI GTMKAC 3 cut(s) 960, 1182, 1194
AccII CGCG 2 cut(s) 1296, 1352
AclI AACGTT 1 cut(s) 954
AclWI GGATC 2 cut(s) 690, 703
AcoI YGGCCR 4 cut(s) 447, 984, 1168, 1568
AcsI RAATTY 3 cut(s) 119, 817, 898
AcuI CTGAAG 1 cut(s) 36
AcvI CACGTG 1 cut(s) 507
AcyI GRCGYC 1 cut(s) 1179
AfaI GTAC 7 cut(s) 278, 503, 538, 659, 863, 1415, 1670
AfiI CCNNNNNNNGG 2 cut(s) 688, 1366
AflIII ACRYGT 2 cut(s) 504, 506
AgsI TTSAA 4 cut(s) 577, 641, 1384, 1528
AhlI ACTAGT 1 cut(s) 722
AjiI CACGTC 2 cut(s) 1453, 1566
AjnI CCWGG 3 cut(s) 830, 1287, 1556
AjuI GAANNNNNNNTTGG 2 cut(s) 545, 577
Alw21I GWGCWC 4 cut(s) 230, 381, 405, 1505
Alw26I GTCTC 4 cut(s) 202, 377, 515, 641
AlwI GGATC 2 cut(s) 690, 703
AlwNI CAGNNNCTG 1 cut(s) 806
Ama87I CYCGRG 1 cut(s) 374
AoxI GGCC 6 cut(s) 447, 984, 1168, 1291, 1396, 1568
ApeKI GCWGC 5 cut(s) 352, 475, 806, 1113, 1214
ApoI RAATTY 3 cut(s) 119, 817, 898
ArsI GACNNNNNNTTYG 2 cut(s) 1187, 1219
AseI ATTAAT 1 cut(s) 1587
Asp700I GAANNNNTTC 1 cut(s) 1277
AspLEI GCGC 4 cut(s) 485, 827, 1498, 1556
AspS9I GGNCC 8 cut(s) 662, 1049, 1090, 1291, 1300, 1396, 1422, 1459
AsuC2I CCSGG 5 cut(s) 242, 630, 1420, 1532, 1572
AsuHPI GGTGA 4 cut(s) 784, 1273, 1336, 1573
AvaI CYCGRG 1 cut(s) 374
AvaII GGWCC 6 cut(s) 662, 1049, 1090, 1300, 1422, 1459
BamHI GGATCC 1 cut(s) 695
BanI GGYRCC 1 cut(s) 1187
BanII GRGCYC 3 cut(s) 230, 381, 405
BbrPI CACGTG 1 cut(s) 507
BbsI GAAGAC 2 cut(s) 1093, 1216
Bbv12I GWGCWC 4 cut(s) 230, 381, 405, 1505
BbvI GCAGC 5 cut(s) 364, 462, 793, 1125, 1226
BccI CCATC 3 cut(s) 797, 911, 1334
BceAI ACGGC 8 cut(s) 555, 979, 980, 1052, 1183, 1201, 1202, 1383
BcgI CGANNNNNNTGC 4 cut(s) 1015, 1049, 1102, 1136
BciT130I CCWGG 3 cut(s) 832, 1289, 1558
BciVI GTATCC 1 cut(s) 1540
BcnI CCSGG 5 cut(s) 242, 630, 1420, 1532, 1572
BcoDI GTCTC 4 cut(s) 202, 377, 515, 641
BcuI ACTAGT 1 cut(s) 722
BfaI CTAG 1 cut(s) 723
BfoI RGCGCY 2 cut(s) 486, 1557
BfuAI ACCTGC 1 cut(s) 593
BfuI GTATCC 1 cut(s) 1540
BglII AGATCT 1 cut(s) 187
BlpI GCTNAGC 2 cut(s) 479, 1499
BmcAI AGTACT 1 cut(s) 1415
Bme1390I CCNGG 8 cut(s) 242, 630, 832, 1289, 1420, 1532, 1558, 1572
Bme18I GGWCC 6 cut(s) 662, 1049, 1090, 1300, 1422, 1459
BmeT110I CYCGRG 1 cut(s) 374
BmgBI CACGTC 2 cut(s) 1453, 1566
BmgT120I GGNCC 8 cut(s) 662, 1049, 1090, 1291, 1300, 1396, 1422, 1459
BmiI GGNNCC 7 cut(s) 664, 680, 697, 813, 1189, 1302, 1461
BmrFI CCNGG 8 cut(s) 242, 630, 832, 1289, 1420, 1532, 1558, 1572
BmsI GCATC 2 cut(s) 321, 603
BoxI GACNNNNGTC 1 cut(s) 369
BpiI GAAGAC 2 cut(s) 1093, 1216
Bpu10I CCTNAGC 1 cut(s) 224
Bpu1102I GCTNAGC 2 cut(s) 479, 1499
BpuEI CTTGAG 3 cut(s) 389, 487, 602
BpuMI CCSGG 5 cut(s) 242, 630, 1420, 1532, 1572
BsaAI YACGTR 1 cut(s) 507
BsaHI GRCGYC 1 cut(s) 1179
BsaJI CCNNGG 7 cut(s) 996, 1287, 1288, 1403, 1531, 1557, 1596
BsaWI WCCGGW 3 cut(s) 1316, 1366, 1658
BsaXI ACNNNNNCTCC 2 cut(s) 1133, 1163
Bsc4I CCNNNNNNNGG 2 cut(s) 688, 1366
Bse118I RCCGGY 2 cut(s) 344, 442
Bse1I ACTGG 2 cut(s) 499, 1159
Bse3DI GCAATG 4 cut(s) 38, 393, 495, 607
BseBI CCWGG 3 cut(s) 832, 1289, 1558
BseDI CCNNGG 7 cut(s) 996, 1287, 1288, 1403, 1531, 1557, 1596
BseGI GGATG 5 cut(s) 60, 66, 126, 562, 1326
BseLI CCNNNNNNNGG 2 cut(s) 688, 1366
BseMI GCAATG 4 cut(s) 38, 393, 495, 607
BseMII CTCAG 6 cut(s) 215, 493, 755, 1056, 1447, 1490
BseNI ACTGG 2 cut(s) 499, 1159
BseRI GAGGAG 1 cut(s) 1020
BseXI GCAGC 5 cut(s) 364, 462, 793, 1125, 1226
BsgI GTGCAG 1 cut(s) 289
Bsh1236I CGCG 2 cut(s) 1296, 1352
Bsh1285I CGRYCG 1 cut(s) 983
BshFI GGCC 6 cut(s) 449, 986, 1170, 1293, 1398, 1570
BshNI GGYRCC 1 cut(s) 1187
BsiEI CGRYCG 1 cut(s) 983
BsiHKAI GWGCWC 4 cut(s) 230, 381, 405, 1505
BsiHKCI CYCGRG 1 cut(s) 374
BslFI GGGAC 1 cut(s) 224
BslI CCNNNNNNNGG 2 cut(s) 688, 1366
BsmAI GTCTC 4 cut(s) 202, 377, 515, 641
BsmBI CGTCTC 1 cut(s) 202
BsmFI GGGAC 1 cut(s) 224
BsnI GGCC 6 cut(s) 449, 986, 1170, 1293, 1398, 1570
BsoBI CYCGRG 1 cut(s) 374
Bsp1286I GDGCHC 4 cut(s) 230, 381, 405, 1505
Bsp143I GATC 4 cut(s) 187, 695, 748, 980
Bsp1720I GCTNAGC 2 cut(s) 479, 1499
BspANI GGCC 6 cut(s) 449, 986, 1170, 1293, 1398, 1570
BspCNI CTCAG 6 cut(s) 216, 492, 754, 1055, 1446, 1491
BspFNI CGCG 2 cut(s) 1296, 1352
BspLI GGNNCC 7 cut(s) 664, 680, 697, 813, 1189, 1302, 1461
BspMI ACCTGC 1 cut(s) 593
BspPI GGATC 2 cut(s) 690, 703
BspQI GCTCTTC 1 cut(s) 386
BspT107I GGYRCC 1 cut(s) 1187
BsrBI CCGCTC 1 cut(s) 1080
BsrDI GCAATG 4 cut(s) 38, 393, 495, 607
BsrFI RCCGGY 2 cut(s) 344, 442
BsrI ACTGG 2 cut(s) 499, 1159
BssAI RCCGGY 2 cut(s) 344, 442
BssECI CCNNGG 7 cut(s) 996, 1287, 1288, 1403, 1531, 1557, 1596
BssMI GATC 4 cut(s) 187, 695, 748, 980
BssNI GRCGYC 1 cut(s) 1179
Bst2UI CCWGG 3 cut(s) 832, 1289, 1558
Bst4CI ACNGT 9 cut(s) 262, 371, 714, 770, 934, 991, 1192, 1426, 1618
Bst6I CTCTTC 1 cut(s) 386
BstACI GRCGYC 1 cut(s) 1179
BstAPI GCANNNNNTGC 1 cut(s) 806
BstBAI YACGTR 1 cut(s) 507
BstC8I GCNNGC 6 cut(s) 339, 357, 444, 590, 1058, 1468
BstDEI CTNAG 7 cut(s) 11, 224, 479, 741, 1042, 1433, 1499
BstDSI CCRYGG 3 cut(s) 996, 1403, 1596
BstEII GGTNACC 1 cut(s) 1342
BstF5I GGATG 5 cut(s) 60, 66, 126, 562, 1326
BstFNI CGCG 2 cut(s) 1296, 1352
BstH2I RGCGCY 2 cut(s) 486, 1557
BstHHI GCGC 4 cut(s) 485, 827, 1498, 1556
BstKTI GATC 4 cut(s) 190, 698, 751, 983
BstMAI GTCTC 4 cut(s) 202, 377, 515, 641
BstMBI GATC 4 cut(s) 187, 695, 748, 980
BstMCI CGRYCG 1 cut(s) 983
BstMWI GCNNNNNNNGC 4 cut(s) 40, 806, 992, 1122
BstNI CCWGG 3 cut(s) 832, 1289, 1558
BstNSI RCATGY 1 cut(s) 168
BstPAI GACNNNNGTC 1 cut(s) 369
BstPI GGTNACC 1 cut(s) 1342
BstSCI CCNGG 8 cut(s) 240, 628, 830, 1287, 1418, 1530, 1556, 1570
BstUI CGCG 2 cut(s) 1296, 1352
BstV1I GCAGC 5 cut(s) 364, 462, 793, 1125, 1226
BstV2I GAAGAC 2 cut(s) 1093, 1216
BstX2I RGATCY 2 cut(s) 187, 695
BstYI RGATCY 2 cut(s) 187, 695
BsuI GTATCC 1 cut(s) 1540
BsuRI GGCC 6 cut(s) 449, 986, 1170, 1293, 1398, 1570
BtgI CCRYGG 3 cut(s) 996, 1403, 1596
BtrI CACGTC 2 cut(s) 1453, 1566
BtsCI GGATG 5 cut(s) 60, 66, 126, 562, 1326
BtsI GCAGTG 1 cut(s) 807
BtsIMutI CAGTG 1 cut(s) 807
BveI ACCTGC 1 cut(s) 593
Cac8I GCNNGC 6 cut(s) 339, 357, 444, 590, 1058, 1468
CaiI CAGNNNCTG 1 cut(s) 806
CfoI GCGC 4 cut(s) 485, 827, 1498, 1556
Cfr10I RCCGGY 2 cut(s) 344, 442
Cfr13I GGNCC 8 cut(s) 662, 1049, 1090, 1291, 1300, 1396, 1422, 1459
CpoI CGGWCCG 1 cut(s) 1422
CseI GACGC 4 cut(s) 184, 481, 806, 1341
CsiI ACCWGGT 1 cut(s) 830
Csp6I GTAC 7 cut(s) 277, 502, 537, 658, 862, 1414, 1669
CspI CGGWCCG 1 cut(s) 1422
CviAII CATG 6 cut(s) 131, 165, 274, 415, 667, 687
CviQI GTAC 7 cut(s) 277, 502, 537, 658, 862, 1414, 1669
DdeI CTNAG 7 cut(s) 11, 224, 479, 741, 1042, 1433, 1499
DpnI GATC 4 cut(s) 189, 697, 750, 982
DpnII GATC 4 cut(s) 187, 695, 748, 980
DrdI GACNNNNNNGTC 1 cut(s) 1097
DseDI GACNNNNNNGTC 1 cut(s) 1097
EaeI YGGCCR 4 cut(s) 447, 984, 1168, 1568
Eam1104I CTCTTC 1 cut(s) 386
EarI CTCTTC 1 cut(s) 386
EciI GGCGGA 1 cut(s) 1145
Ecl136II GAGCTC 3 cut(s) 228, 379, 403
Eco24I GRGCYC 3 cut(s) 230, 381, 405
Eco47I GGWCC 6 cut(s) 662, 1049, 1090, 1300, 1422, 1459
Eco53kI GAGCTC 3 cut(s) 228, 379, 403
Eco57I CTGAAG 1 cut(s) 36
Eco72I CACGTG 1 cut(s) 507
Eco88I CYCGRG 1 cut(s) 374
Eco91I GGTNACC 1 cut(s) 1342
EcoICRI GAGCTC 3 cut(s) 228, 379, 403
EcoO109I RGGNCCY 1 cut(s) 1300
EcoO65I GGTNACC 1 cut(s) 1342
EcoRII CCWGG 3 cut(s) 830, 1287, 1556
EcoT38I GRGCYC 3 cut(s) 230, 381, 405
Esp3I CGTCTC 1 cut(s) 202
FaeI CATG 6 cut(s) 134, 168, 277, 418, 670, 690
FalI AAGNNNNNCTT 2 cut(s) 1262, 1294
FaqI GGGAC 1 cut(s) 224
FatI CATG 6 cut(s) 130, 164, 273, 414, 666, 686
FauI CCCGC 1 cut(s) 1459
FblI GTMKAC 3 cut(s) 960, 1182, 1194
FokI GGATG 5 cut(s) 47, 53, 113, 569, 1313
FriOI GRGCYC 3 cut(s) 230, 381, 405
FspBI CTAG 1 cut(s) 723
GlaI GCGC 4 cut(s) 484, 826, 1497, 1555
HaeII RGCGCY 2 cut(s) 486, 1557
HaeIII GGCC 6 cut(s) 449, 986, 1170, 1293, 1398, 1570
HgaI GACGC 4 cut(s) 184, 481, 806, 1341
HhaI GCGC 4 cut(s) 485, 827, 1498, 1556
Hin1I GRCGYC 1 cut(s) 1179
Hin1II CATG 6 cut(s) 134, 168, 277, 418, 670, 690
Hin6I GCGC 4 cut(s) 483, 825, 1496, 1554
HinP1I GCGC 4 cut(s) 483, 825, 1496, 1554
HincII GTYRAC 3 cut(s) 265, 961, 1195
HindII GTYRAC 3 cut(s) 265, 961, 1195
HinfI GANTC 5 cut(s) 524, 625, 739, 1320, 1606
HphI GGTGA 4 cut(s) 784, 1273, 1336, 1573
Hpy166II GTNNAC 9 cut(s) 265, 504, 961, 1147, 1183, 1195, 1377, 1459, 1614
Hpy188I TCNGA 8 cut(s) 421, 695, 738, 980, 1004, 1090, 1357, 1546
Hpy8I GTNNAC 9 cut(s) 265, 504, 961, 1147, 1183, 1195, 1377, 1459, 1614
HpyAV CCTTC 6 cut(s) 9, 67, 509, 547, 1042, 1402
HpyCH4III ACNGT 9 cut(s) 262, 371, 714, 770, 934, 991, 1192, 1426, 1618
HpyCH4V TGCA 6 cut(s) 306, 588, 718, 1113, 1236, 1338
HpyF10VI GCNNNNNNNGC 4 cut(s) 40, 806, 992, 1122
HpyF3I CTNAG 7 cut(s) 11, 224, 479, 741, 1042, 1433, 1499
Hsp92I GRCGYC 1 cut(s) 1179
Hsp92II CATG 6 cut(s) 134, 168, 277, 418, 670, 690
HspAI GCGC 4 cut(s) 483, 825, 1496, 1554
KroI GCCGGC 1 cut(s) 442
KroNI GCCGGC 1 cut(s) 444
Kzo9I GATC 4 cut(s) 187, 695, 748, 980
LguI GCTCTTC 1 cut(s) 386
LmnI GCTCC 3 cut(s) 817, 1085, 1598
Lsp1109I GCAGC 5 cut(s) 364, 462, 793, 1125, 1226
LweI GCATC 2 cut(s) 321, 603
MabI ACCWGGT 1 cut(s) 830
MaeI CTAG 1 cut(s) 723
MaeIII GTNAC 2 cut(s) 1342, 1561
MalI GATC 4 cut(s) 189, 697, 750, 982
MbiI CCGCTC 1 cut(s) 1080
MboI GATC 4 cut(s) 187, 695, 748, 980
MboII GAAGA 9 cut(s) 373, 634, 904, 907, 1093, 1195, 1216, 1270, 1282
MflI RGATCY 2 cut(s) 187, 695
MhlI GDGCHC 4 cut(s) 230, 381, 405, 1505
MluCI AATT 5 cut(s) 119, 560, 817, 898, 1651
MlyI GAGTC 2 cut(s) 733, 1615
MmeI TCCRAC 7 cut(s) 71, 324, 673, 982, 1380, 1455, 1524
MreI CGCCGGCG 1 cut(s) 442
MroNI GCCGGC 1 cut(s) 442
MroXI GAANNNNTTC 1 cut(s) 1277
MseI TTAA 2 cut(s) 1587, 1682
MspR9I CCNGG 8 cut(s) 242, 630, 832, 1289, 1420, 1532, 1558, 1572
MvaI CCWGG 3 cut(s) 832, 1289, 1558
MvnI CGCG 2 cut(s) 1296, 1352
MwoI GCNNNNNNNGC 4 cut(s) 40, 806, 992, 1122
NaeI GCCGGC 1 cut(s) 444
NciI CCSGG 5 cut(s) 242, 630, 1420, 1532, 1572
NdeII GATC 4 cut(s) 187, 695, 748, 980
NgoMIV GCCGGC 1 cut(s) 442
NlaIII CATG 6 cut(s) 134, 168, 277, 418, 670, 690
NlaIV GGNNCC 7 cut(s) 664, 680, 697, 813, 1189, 1302, 1461
NmeAIII GCCGAG 1 cut(s) 424
NmuCI GTSAC 2 cut(s) 1342, 1561
NspI RCATGY 1 cut(s) 168
PaeR7I CTCGAG 1 cut(s) 374
PasI CCCWGGG 1 cut(s) 1288
PciSI GCTCTTC 1 cut(s) 386
PcsI WCGNNNNNNNCGW 7 cut(s) 372, 381, 1173, 1191, 1218, 1385, 1622
PdiI GCCGGC 1 cut(s) 444
PdmI GAANNNNTTC 1 cut(s) 1277
PfeI GAWTC 3 cut(s) 524, 625, 1320
PfoI TCCNGGA 3 cut(s) 240, 628, 1418
Ple19I CGATCG 1 cut(s) 983
PleI GAGTC 2 cut(s) 733, 1614
PmaCI CACGTG 1 cut(s) 507
PmlI CACGTG 1 cut(s) 507
PpsI GAGTC 2 cut(s) 733, 1614
Ppu21I YACGTR 1 cut(s) 507
PpuMI RGGWCCY 1 cut(s) 1300
PshAI GACNNNNGTC 1 cut(s) 369
PshBI ATTAAT 1 cut(s) 1587
Psp124BI GAGCTC 3 cut(s) 230, 381, 405
Psp1406I AACGTT 1 cut(s) 954
Psp5II RGGWCCY 1 cut(s) 1300
Psp6I CCWGG 3 cut(s) 830, 1287, 1556
PspCI CACGTG 1 cut(s) 507
PspEI GGTNACC 1 cut(s) 1342
PspGI CCWGG 3 cut(s) 830, 1287, 1556
PspN4I GGNNCC 7 cut(s) 664, 680, 697, 813, 1189, 1302, 1461
PspPI GGNCC 8 cut(s) 662, 1049, 1090, 1291, 1300, 1396, 1422, 1459
PspPPI RGGWCCY 1 cut(s) 1300
PstNI CAGNNNCTG 1 cut(s) 806
PsuI RGATCY 2 cut(s) 187, 695
PvuI CGATCG 1 cut(s) 983
RsaI GTAC 7 cut(s) 278, 503, 538, 659, 863, 1415, 1670
RsaNI GTAC 7 cut(s) 277, 502, 537, 658, 862, 1414, 1669
Rsr2I CGGWCCG 1 cut(s) 1422
RsrII CGGWCCG 1 cut(s) 1422
SacI GAGCTC 3 cut(s) 230, 381, 405
SalI GTCGAC 2 cut(s) 959, 1193
SapI GCTCTTC 1 cut(s) 386
SaqAI TTAA 2 cut(s) 1587, 1682
Sau3AI GATC 4 cut(s) 187, 695, 748, 980
Sau96I GGNCC 8 cut(s) 662, 1049, 1090, 1291, 1300, 1396, 1422, 1459
ScaI AGTACT 1 cut(s) 1415
SchI GAGTC 2 cut(s) 733, 1615
ScrFI CCNGG 8 cut(s) 242, 630, 832, 1289, 1420, 1532, 1558, 1572
SduI GDGCHC 4 cut(s) 230, 381, 405, 1505
SexAI ACCWGGT 1 cut(s) 830
SfaNI GCATC 2 cut(s) 321, 603
Sfr274I CTCGAG 1 cut(s) 374
SgrAI CRCCGGYG 1 cut(s) 442
SinI GGWCC 6 cut(s) 662, 1049, 1090, 1300, 1422, 1459
SlaI CTCGAG 1 cut(s) 374
SmlI CTYRAG 4 cut(s) 374, 404, 466, 617
SmoI CTYRAG 4 cut(s) 374, 404, 466, 617
SpeI ACTAGT 1 cut(s) 722
Sse9I AATT 5 cut(s) 119, 560, 817, 898, 1651
SspMI CTAG 1 cut(s) 723
SstI GAGCTC 3 cut(s) 230, 381, 405
StyD4I CCNGG 8 cut(s) 240, 628, 830, 1287, 1418, 1530, 1556, 1570
TaaI ACNGT 9 cut(s) 262, 371, 714, 770, 934, 991, 1192, 1426, 1618
TasI AATT 5 cut(s) 119, 560, 817, 898, 1651
TatI WGTACW 2 cut(s) 501, 1413
TauI GCSGC 6 cut(s) 344, 449, 488, 812, 1296, 1352
TfiI GAWTC 3 cut(s) 524, 625, 1320
Tru1I TTAA 2 cut(s) 1587, 1682
Tru9I TTAA 2 cut(s) 1587, 1682
TscAI CASTG 1 cut(s) 807
TseFI GTSAC 2 cut(s) 1342, 1561
TseI GCWGC 5 cut(s) 352, 475, 806, 1113, 1214
Tsp45I GTSAC 2 cut(s) 1342, 1561
TspDTI ATGAA 5 cut(s) 49, 573, 683, 1003, 1189
TspGWI ACGGA 5 cut(s) 675, 1087, 1392, 1613, 1656
TspRI CASTG 1 cut(s) 807
VpaK11BI GGWCC 6 cut(s) 662, 1049, 1090, 1300, 1422, 1459
VspI ATTAAT 1 cut(s) 1587
XapI RAATTY 3 cut(s) 119, 817, 898
XceI RCATGY 1 cut(s) 168
XcmI CCANNNNNNNNNTGG 1 cut(s) 994
XhoI CTCGAG 1 cut(s) 374
XmiI GTMKAC 3 cut(s) 960, 1182, 1194
XmnI GAANNNNTTC 1 cut(s) 1277
XspI CTAG 1 cut(s) 723
ZraI GACGTC 1 cut(s) 1180
ZrmI AGTACT 1 cut(s) 1415
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.