Prupe.8G264100_v2.0.a1

pectinesterase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
prunus_persica
Pp08
OG0014882
Physical Location & Seq
Forward (+)
22169289 .. 22173300
4012 bp
Loading structure...
UTR
Exon/CDS
Intron
Prupe.8G264100.1

Sequence Viewer

Length: 1782 bp
ATGCCCTCCTCCCCACCTCTCTCGGTCTTTGTCTCACACAAACACATGAGTTTTCCTTCGAGCTTACACACCACAACCACCATGTATAATCTAAGAAGGAGAACAAAACTCTTGCTCTCTGTGCTCCCCACTTCAGCTCTCCTCCTAATCCTTCTCTTTTCAAATACCCATTTGAGAAATTCAAACTCAATCACCCCCAAAACACACGTCCACAACCGCATTCAATTAGCAGCCCACTCGGCATGCCAAGACACACTCTACAAAGACCTCTGCGTCTCCACCCTCTCCACCTTCCCAGATCTCTCCACCAAATCAGTCCCACAGATCATATCCTACACCGTCTCCCACACCGTCTCGGAGGTCCAAGCCTCCTCCTCCAACTGCACCGGCATTATCAAAAAACAGTTCAAGAAGCTCGACGCCCTTCAGCAAATAGCTCTCGACGACTGCCTCGAGCTCTTCGACGACACCATTGCCGAGCTCAAAGCAGTCATCTCTGACCTCTCCGTCGAGAAGCTCAGCTCCGACCGCTACTACGACTTGAAGACCTTGCTCAGCGGCGCAATGACCAACCAGTACACGTGTCTTGATGGGTTTAACGACACTGGAATTGTGAGGCATGAAATTCAAAGCAGATTGCAGACCATCTCTCAGCACGTGAGCAATGCCCTGGTCATGCTCAAGAAGATTCCCGGAGCTCTTAAGTACGAGAACTCTAAGTCGTCGACGTCGTCGGAGATGTTCCCTGAGTTTGGACCCATGAGAAATGGGTTCCCTACTTGGGTTTCATCGAAAGATCGAAAGCTCTTGCAGTCCGTGGTGAATGGGACAAAATACGATTTGGTGGTGGCCAAGGACGGCACTGGAAACTTCACTACTATCAGCGAGGCTGTAGCCGTGGCTCCAAATTCAAGTGCAACCAGGTTTGTGATATACATAAAAAGCGGGGCATATTTTGAGAATGTGGAGGTTGTTAGGAAGAAGACGAAGTTGATGTTCTTGGGTGATGGGATTGGAAAGACGGTTGTGAAAGCCAGTAGGAATGTGGTGGATGGCTGGACTACTTTCCGATCAGCCACAGTCGCTGTTGTGGGAGACGGGTTCATAGCCCAAGGCATTACGTTTGAGAACGCAGCAGGCCCAAGCAAACACCAAGCGGTAGCCTTTAGGAGCGGCTCTGATTTCTCTGCATTCTACAAGTGCAGCTTCGTGGGCTACCAAGACACCCTCTACGTTCATTCCCTCCGCCAATTCTACCGCGAATGCGACATCTACGGCACCGTCGATTTCATCTTCGGCAACGCCGCCGTAGTCCTCCAGAACTGCAACCTCTACGCGCGCAAACCAAACGAAAACCAGAAGAACATGTTCACGGCCCAAGGCCGTGAGGACCCCAACCAGAACACTGGCATTTCCATCTTGAACTCCAAAGTCGCAGCCGCTTCTGATCTGGTCCCGGTCAAGGCAATGTTCAAAACGTATCTGGGCCGTCCGTGGAAAGAGTATTCTAGGACGGTTTACATGAAATCATACATTGATGATTTGGTGGAGCCCGCTGGTTGGCTGGAGTGGAATGGCACGTTTGCATTGAGTACGTTGTATTATGGGGAGTATTTGAACCGAGGACCCGGTTCGAATACAAGCGCGAGGGTGACGTGGCCGGGTTACCGGGTCATCAACAGCTCAGCTGAGGCGAATCAGTTTACGGTTGGGCCGTTTATACAAGGAAATGAGTGGCTCAATGCTACCAATATTCCTTACTTCATTGGTTTGACTCCTTGA

Protein Analysis

594

Amino Acids

65.73

Weight (kDa)

9.03

Isoelectric Point (pI)

30.38

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0014882)

Species Orthologous Gene IDs
arabidopsis_thaliana AT4G02300 AT4G02320
fragaria_vesca FvH4_2g25980
malus_domestica MD11G1307200.v1.1
prunus_persica Prupe.8G264100_v2.0.a1
rosa_chinensis RchiOBHm_Chr6g0294341
rosa_laevigata RLG00000011879
rosa_multiflora Rmu_co8392343.1_g000001 Rmu_sc0005369.1_g000009
rosa_roxburghii Rroxscaffold_7G00173460
rosa_rugosa Rorug06G0243600
rosa_samantha Rh6AG356200 Rh6BG362900 Rh6CG370200 Rh6DG356700
rosa_wichuraiana Rw6G031040

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 2 cut(s) 272, 506
AatII GACGTC 1 cut(s) 731
AccB1I GGYRCC 1 cut(s) 1277
AccBSI CCGCTC 1 cut(s) 1173
AccI GTMKAC 1 cut(s) 725
AccII CGCG 4 cut(s) 1260, 1337, 1339, 1646
AcoI YGGCCR 2 cut(s) 849, 1658
AcsI RAATTY 3 cut(s) 178, 624, 907
AcuI CTGAAG 2 cut(s) 117, 410
AcvI CACGTG 2 cut(s) 582, 658
AcyI GRCGYC 2 cut(s) 420, 728
AfaI GTAC 3 cut(s) 578, 707, 1594
AfiI CCNNNNNNNGG 4 cut(s) 752, 781, 1462, 1560
AflII CTTAAG 1 cut(s) 701
AflIII ACRYGT 4 cut(s) 205, 579, 581, 1365
AjiI CACGTC 2 cut(s) 208, 1656
AjnI CCWGG 2 cut(s) 669, 920
Alw21I GWGCWC 4 cut(s) 126, 459, 483, 700
Alw26I GTCTC 5 cut(s) 37, 280, 346, 358, 1089
AlwNI CAGNNNCTG 1 cut(s) 1085
Ama87I CYCGRG 1 cut(s) 452
AoxI GGCC 7 cut(s) 849, 1138, 1374, 1381, 1486, 1658, 1712
ApeKI GCWGC 4 cut(s) 230, 1133, 1203, 1436
ApoI RAATTY 3 cut(s) 178, 624, 907
ArsI GACNNNNNNTTYG 2 cut(s) 192, 224
Asp700I GAANNNNTTC 1 cut(s) 1367
AspLEI GCGC 4 cut(s) 563, 1339, 1341, 1646
AspS9I GGNCC 9 cut(s) 361, 755, 1139, 1375, 1390, 1453, 1486, 1625, 1712
AsuC2I CCSGG 5 cut(s) 693, 1457, 1629, 1662, 1670
AsuHPI GGTGA 4 cut(s) 184, 832, 1016, 1663
AsuII TTCGAA 1 cut(s) 1634
AvaI CYCGRG 1 cut(s) 452
AvaII GGWCC 5 cut(s) 361, 755, 1390, 1453, 1625
BalI TGGCCA 1 cut(s) 851
BanI GGYRCC 1 cut(s) 1277
BanII GRGCYC 4 cut(s) 459, 483, 700, 1554
BbrPI CACGTG 2 cut(s) 582, 658
BbsI GAAGAC 2 cut(s) 551, 989
Bbv12I GWGCWC 4 cut(s) 126, 459, 483, 700
BbvCI CCTCAGC 1 cut(s) 1689
BbvI GCAGC 4 cut(s) 242, 1145, 1215, 1448
BccI CCATC 5 cut(s) 584, 653, 1001, 1046, 1424
BceAI ACGGC 8 cut(s) 874, 881, 1291, 1292, 1368, 1389, 1473, 1699
BcgI CGANNNNNNTGC 4 cut(s) 219, 253, 455, 489
BciT130I CCWGG 2 cut(s) 671, 922
BcnI CCSGG 5 cut(s) 693, 1457, 1629, 1662, 1670
BcoDI GTCTC 5 cut(s) 37, 280, 346, 358, 1089
BfaI CTAG 1 cut(s) 1509
BfmI CTRYAG 1 cut(s) 891
BfrI CTTAAG 1 cut(s) 701
BglI GCCNNNNNGGC 1 cut(s) 239
BglII AGATCT 1 cut(s) 298
BisI GCNGC 8 cut(s) 231, 559, 1134, 1174, 1204, 1305, 1437, 1440
BlpI GCTNAGC 3 cut(s) 518, 554, 1684
BlsI GCNGC 8 cut(s) 232, 560, 1135, 1175, 1205, 1306, 1438, 1441
Bme1390I CCNGG 7 cut(s) 671, 693, 922, 1457, 1629, 1662, 1670
Bme18I GGWCC 5 cut(s) 361, 755, 1390, 1453, 1625
BmeT110I CYCGRG 1 cut(s) 452
BmgBI CACGTC 2 cut(s) 208, 1656
BmgT120I GGNCC 9 cut(s) 361, 755, 1139, 1375, 1390, 1453, 1486, 1625, 1712
BmiI GGNNCC 8 cut(s) 757, 773, 903, 1279, 1392, 1455, 1551, 1627
BmrFI CCNGG 7 cut(s) 671, 693, 922, 1457, 1629, 1662, 1670
BpiI GAAGAC 2 cut(s) 551, 989
BpmI CTGGAG 2 cut(s) 1301, 1586
Bpu10I CCTNAGC 1 cut(s) 1689
Bpu1102I GCTNAGC 3 cut(s) 518, 554, 1684
Bpu14I TTCGAA 1 cut(s) 1634
BpuEI CTTGAG 1 cut(s) 665
BpuMI CCSGG 5 cut(s) 693, 1457, 1629, 1662, 1670
BsaAI YACGTR 2 cut(s) 582, 658
BsaHI GRCGYC 2 cut(s) 420, 728
BsaJI CCNNGG 8 cut(s) 669, 816, 852, 897, 1111, 1378, 1493, 1621
BsaXI ACNNNNNCTCC 2 cut(s) 326, 356
Bsc4I CCNNNNNNNGG 4 cut(s) 752, 781, 1462, 1560
Bse118I RCCGGY 1 cut(s) 386
Bse1I ACTGG 5 cut(s) 574, 610, 868, 1035, 1411
Bse3DI GCAATG 4 cut(s) 471, 570, 670, 1473
BseBI CCWGG 2 cut(s) 671, 922
BseDI CCNNGG 8 cut(s) 669, 816, 852, 897, 1111, 1378, 1493, 1621
BseGI GGATG 1 cut(s) 1057
BseLI CCNNNNNNNGG 4 cut(s) 752, 781, 1462, 1560
BseMI GCAATG 4 cut(s) 471, 570, 670, 1473
BseMII CTCAG 6 cut(s) 532, 568, 665, 738, 1680, 1698
BseNI ACTGG 5 cut(s) 574, 610, 868, 1035, 1411
BsePI GCGCGC 1 cut(s) 1337
BseRI GAGGAG 3 cut(s) 131, 361, 364
BseXI GCAGC 4 cut(s) 242, 1145, 1215, 1448
BsgI GTGCAG 2 cut(s) 367, 1222
Bsh1236I CGCG 4 cut(s) 1260, 1337, 1339, 1646
Bsh1285I CGRYCG 1 cut(s) 529
BshFI GGCC 7 cut(s) 851, 1140, 1376, 1383, 1488, 1660, 1714
BshNI GGYRCC 1 cut(s) 1277
BsiEI CGRYCG 1 cut(s) 529
BsiHKAI GWGCWC 4 cut(s) 126, 459, 483, 700
BsiHKCI CYCGRG 1 cut(s) 452
BsiSI CCGG 6 cut(s) 387, 693, 1457, 1629, 1661, 1669
BslFI GGGAC 3 cut(s) 302, 841, 1439
BslI CCNNNNNNNGG 4 cut(s) 752, 781, 1462, 1560
BsmAI GTCTC 5 cut(s) 37, 280, 346, 358, 1089
BsmBI CGTCTC 4 cut(s) 280, 346, 358, 1089
BsmFI GGGAC 3 cut(s) 302, 841, 1439
BsmI GAATGC 3 cut(s) 219, 1190, 1268
BsnI GGCC 7 cut(s) 851, 1140, 1376, 1383, 1488, 1660, 1714
BsoBI CYCGRG 1 cut(s) 452
Bsp119I TTCGAA 1 cut(s) 1634
Bsp1286I GDGCHC 5 cut(s) 126, 459, 483, 700, 1554
Bsp143I GATC 5 cut(s) 298, 324, 796, 1070, 1447
Bsp1720I GCTNAGC 3 cut(s) 518, 554, 1684
BspANI GGCC 7 cut(s) 851, 1140, 1376, 1383, 1488, 1660, 1714
BspCNI CTCAG 6 cut(s) 531, 567, 664, 739, 1681, 1697
BspFNI CGCG 4 cut(s) 1260, 1337, 1339, 1646
BspLI GGNNCC 8 cut(s) 757, 773, 903, 1279, 1392, 1455, 1551, 1627
BspQI GCTCTTC 1 cut(s) 464
BspT104I TTCGAA 1 cut(s) 1634
BspT107I GGYRCC 1 cut(s) 1277
BspTI CTTAAG 1 cut(s) 701
BsrBI CCGCTC 1 cut(s) 1173
BsrDI GCAATG 4 cut(s) 471, 570, 670, 1473
BsrFI RCCGGY 1 cut(s) 386
BsrI ACTGG 5 cut(s) 574, 610, 868, 1035, 1411
BssAI RCCGGY 1 cut(s) 386
BssECI CCNNGG 8 cut(s) 669, 816, 852, 897, 1111, 1378, 1493, 1621
BssHII GCGCGC 1 cut(s) 1337
BssMI GATC 5 cut(s) 298, 324, 796, 1070, 1447
BssNI GRCGYC 2 cut(s) 420, 728
BssT1I CCWWGG 3 cut(s) 852, 1111, 1378
Bst2UI CCWGG 2 cut(s) 671, 922
Bst4CI ACNGT 8 cut(s) 340, 352, 405, 1024, 1081, 1282, 1516, 1708
Bst6I CTCTTC 1 cut(s) 464
BstACI GRCGYC 2 cut(s) 420, 728
BstAFI CTTAAG 1 cut(s) 701
BstBAI YACGTR 2 cut(s) 582, 658
BstBI TTCGAA 1 cut(s) 1634
BstC8I GCNNGC 4 cut(s) 244, 1138, 1339, 1554
BstDEI CTNAG 8 cut(s) 92, 518, 554, 651, 717, 747, 1684, 1689
BstDSI CCRYGG 3 cut(s) 816, 897, 1493
BstEII GGTNACC 1 cut(s) 1664
BstF5I GGATG 1 cut(s) 1057
BstFNI CGCG 4 cut(s) 1260, 1337, 1339, 1646
BstHHI GCGC 4 cut(s) 563, 1339, 1341, 1646
BstKTI GATC 5 cut(s) 301, 327, 799, 1073, 1450
BstMAI GTCTC 5 cut(s) 37, 280, 346, 358, 1089
BstMBI GATC 5 cut(s) 298, 324, 796, 1070, 1447
BstMCI CGRYCG 1 cut(s) 529
BstMWI GCNNNNNNNGC 5 cut(s) 121, 239, 528, 1082, 1212
BstNI CCWGG 2 cut(s) 671, 922
BstNSI RCATGY 2 cut(s) 246, 1369
BstPI GGTNACC 1 cut(s) 1664
BstSCI CCNGG 7 cut(s) 669, 691, 920, 1455, 1627, 1660, 1668
BstSFI CTRYAG 1 cut(s) 891
BstUI CGCG 4 cut(s) 1260, 1337, 1339, 1646
BstV1I GCAGC 4 cut(s) 242, 1145, 1215, 1448
BstV2I GAAGAC 2 cut(s) 551, 989
BstX2I RGATCY 1 cut(s) 298
BstXI CCANNNNNNTGG 1 cut(s) 1406
BstYI RGATCY 1 cut(s) 298
BsuRI GGCC 7 cut(s) 851, 1140, 1376, 1383, 1488, 1660, 1714
BtgI CCRYGG 3 cut(s) 816, 897, 1493
BtrI CACGTC 2 cut(s) 208, 1656
BtsCI GGATG 1 cut(s) 1057
BtsIMutI CAGTG 3 cut(s) 603, 861, 1404
Cac8I GCNNGC 4 cut(s) 244, 1138, 1339, 1554
CaiI CAGNNNCTG 1 cut(s) 1085
CfoI GCGC 4 cut(s) 563, 1339, 1341, 1646
Cfr10I RCCGGY 1 cut(s) 386
Cfr13I GGNCC 9 cut(s) 361, 755, 1139, 1375, 1390, 1453, 1486, 1625, 1712
CseI GACGC 2 cut(s) 262, 428
CsiI ACCWGGT 1 cut(s) 920
Csp6I GTAC 3 cut(s) 577, 706, 1593
CviAII CATG 8 cut(s) 46, 82, 243, 620, 676, 760, 1366, 1522
CviQI GTAC 3 cut(s) 577, 706, 1593
DdeI CTNAG 8 cut(s) 92, 518, 554, 651, 717, 747, 1684, 1689
DpnI GATC 5 cut(s) 300, 326, 798, 1072, 1449
DpnII GATC 5 cut(s) 298, 324, 796, 1070, 1447
DrdI GACNNNNNNGTC 2 cut(s) 272, 506
DseDI GACNNNNNNGTC 2 cut(s) 272, 506
EaeI YGGCCR 2 cut(s) 849, 1658
Eam1104I CTCTTC 1 cut(s) 464
EarI CTCTTC 1 cut(s) 464
EciI GGCGGA 1 cut(s) 1235
Ecl136II GAGCTC 3 cut(s) 457, 481, 698
Eco130I CCWWGG 3 cut(s) 852, 1111, 1378
Eco24I GRGCYC 4 cut(s) 459, 483, 700, 1554
Eco47I GGWCC 5 cut(s) 361, 755, 1390, 1453, 1625
Eco53kI GAGCTC 3 cut(s) 457, 481, 698
Eco57I CTGAAG 2 cut(s) 117, 410
Eco72I CACGTG 2 cut(s) 582, 658
Eco88I CYCGRG 1 cut(s) 452
Eco91I GGTNACC 1 cut(s) 1664
EcoICRI GAGCTC 3 cut(s) 457, 481, 698
EcoO109I RGGNCCY 2 cut(s) 1390, 1625
EcoO65I GGTNACC 1 cut(s) 1664
EcoRII CCWGG 2 cut(s) 669, 920
EcoT14I CCWWGG 3 cut(s) 852, 1111, 1378
EcoT38I GRGCYC 4 cut(s) 459, 483, 700, 1554
ErhI CCWWGG 3 cut(s) 852, 1111, 1378
Esp3I CGTCTC 4 cut(s) 280, 346, 358, 1089
FaeI CATG 8 cut(s) 49, 85, 246, 623, 679, 763, 1369, 1525
FalI AAGNNNNNCTT 2 cut(s) 1190, 1222
FaqI GGGAC 3 cut(s) 302, 841, 1439
FatI CATG 8 cut(s) 45, 81, 242, 619, 675, 759, 1365, 1521
FauI CCCGC 2 cut(s) 938, 1561
FblI GTMKAC 1 cut(s) 725
Fnu4HI GCNGC 8 cut(s) 231, 559, 1134, 1174, 1204, 1305, 1437, 1440
FokI GGATG 1 cut(s) 1064
FriOI GRGCYC 4 cut(s) 459, 483, 700, 1554
Fsp4HI GCNGC 8 cut(s) 231, 559, 1134, 1174, 1204, 1305, 1437, 1440
FspBI CTAG 1 cut(s) 1509
GlaI GCGC 4 cut(s) 562, 1338, 1340, 1645
GluI GCNGC 8 cut(s) 231, 559, 1134, 1174, 1204, 1305, 1437, 1440
GsuI CTGGAG 2 cut(s) 1301, 1586
HaeIII GGCC 7 cut(s) 851, 1140, 1376, 1383, 1488, 1660, 1714
HapII CCGG 6 cut(s) 387, 693, 1457, 1629, 1661, 1669
HgaI GACGC 2 cut(s) 262, 428
HhaI GCGC 4 cut(s) 563, 1339, 1341, 1646
Hin1I GRCGYC 2 cut(s) 420, 728
Hin1II CATG 8 cut(s) 49, 85, 246, 623, 679, 763, 1369, 1525
Hin6I GCGC 4 cut(s) 561, 1337, 1339, 1644
HinP1I GCGC 4 cut(s) 561, 1337, 1339, 1644
HincII GTYRAC 1 cut(s) 726
HindII GTYRAC 1 cut(s) 726
HinfI GANTC 3 cut(s) 688, 1696, 1774
HpaII CCGG 6 cut(s) 387, 693, 1457, 1629, 1661, 1669
HphI GGTGA 4 cut(s) 184, 832, 1016, 1663
Hpy166II GTNNAC 6 cut(s) 211, 579, 726, 1371, 1519, 1704
Hpy188I TCNGA 7 cut(s) 358, 499, 526, 736, 1070, 1180, 1447
Hpy188III TCNNGA 7 cut(s) 409, 440, 511, 587, 682, 1318, 1420
Hpy8I GTNNAC 6 cut(s) 211, 579, 726, 1371, 1519, 1704
Hpy99I CGWCG 9 cut(s) 422, 446, 467, 512, 727, 730, 733, 736, 1286
HpyAV CCTTC 5 cut(s) 66, 90, 161, 301, 434
HpyCH4III ACNGT 8 cut(s) 340, 352, 405, 1024, 1081, 1282, 1516, 1708
HpyCH4V TGCA 8 cut(s) 384, 640, 811, 917, 1190, 1203, 1326, 1586
HpyF10VI GCNNNNNNNGC 5 cut(s) 121, 239, 528, 1082, 1212
HpyF3I CTNAG 8 cut(s) 92, 518, 554, 651, 717, 747, 1684, 1689
Hsp92I GRCGYC 2 cut(s) 420, 728
Hsp92II CATG 8 cut(s) 49, 85, 246, 623, 679, 763, 1369, 1525
HspAI GCGC 4 cut(s) 561, 1337, 1339, 1644
Kzo9I GATC 5 cut(s) 298, 324, 796, 1070, 1447
LguI GCTCTTC 1 cut(s) 464
LmnI GCTCC 6 cut(s) 129, 527, 695, 907, 1170, 1549
Lsp1109I GCAGC 4 cut(s) 242, 1145, 1215, 1448
MabI ACCWGGT 1 cut(s) 920
MaeI CTAG 1 cut(s) 1509
MaeIII GTNAC 2 cut(s) 1651, 1664
MalI GATC 5 cut(s) 300, 326, 798, 1072, 1449
MbiI CCGCTC 1 cut(s) 1173
MboI GATC 5 cut(s) 298, 324, 796, 1070, 1447
MboII GAAGA 7 cut(s) 451, 556, 697, 991, 994, 1285, 1372
MflI RGATCY 1 cut(s) 298
MhlI GDGCHC 5 cut(s) 126, 459, 483, 700, 1554
MlsI TGGCCA 1 cut(s) 851
MluCI AATT 6 cut(s) 178, 224, 609, 624, 907, 1250
MluNI TGGCCA 1 cut(s) 851
MlyI GAGTC 1 cut(s) 1768
MmeI TCCRAC 3 cut(s) 402, 549, 714
Mox20I TGGCCA 1 cut(s) 851
MroXI GAANNNNTTC 1 cut(s) 1367
MscI TGGCCA 1 cut(s) 851
MseI TTAA 2 cut(s) 597, 702
Msp20I TGGCCA 1 cut(s) 851
MspA1I CMGCKG 3 cut(s) 558, 1556, 1688
MspCI CTTAAG 1 cut(s) 701
MspI CCGG 6 cut(s) 387, 693, 1457, 1629, 1661, 1669
MspR9I CCNGG 7 cut(s) 671, 693, 922, 1457, 1629, 1662, 1670
Mva1269I GAATGC 3 cut(s) 219, 1190, 1268
MvaI CCWGG 2 cut(s) 671, 922
MvnI CGCG 4 cut(s) 1260, 1337, 1339, 1646
MwoI GCNNNNNNNGC 5 cut(s) 121, 239, 528, 1082, 1212
NciI CCSGG 5 cut(s) 693, 1457, 1629, 1662, 1670
NdeII GATC 5 cut(s) 298, 324, 796, 1070, 1447
NlaIII CATG 8 cut(s) 49, 85, 246, 623, 679, 763, 1369, 1525
NlaIV GGNNCC 8 cut(s) 757, 773, 903, 1279, 1392, 1455, 1551, 1627
NmeAIII GCCGAG 2 cut(s) 218, 502
NmuCI GTSAC 1 cut(s) 1651
NspI RCATGY 2 cut(s) 246, 1369
NspV TTCGAA 1 cut(s) 1634
PaeI GCATGC 1 cut(s) 246
PaeR7I CTCGAG 1 cut(s) 452
PauI GCGCGC 1 cut(s) 1337
PciI ACATGT 1 cut(s) 1365
PciSI GCTCTTC 1 cut(s) 464
PcsI WCGNNNNNNNCGW 5 cut(s) 450, 459, 728, 1281, 1712
PctI GAATGC 3 cut(s) 219, 1190, 1268
PdmI GAANNNNTTC 1 cut(s) 1367
PfeI GAWTC 2 cut(s) 688, 1696
PflFI GACNNNGTC 1 cut(s) 730
PfoI TCCNGGA 1 cut(s) 691
PkrI GCNGC 8 cut(s) 232, 560, 1135, 1175, 1205, 1306, 1438, 1441
PleI GAGTC 1 cut(s) 1768
PmaCI CACGTG 2 cut(s) 582, 658
PmlI CACGTG 2 cut(s) 582, 658
PpsI GAGTC 1 cut(s) 1768
Ppu21I YACGTR 2 cut(s) 582, 658
PpuMI RGGWCCY 2 cut(s) 1390, 1625
PscI ACATGT 1 cut(s) 1365
Psp124BI GAGCTC 3 cut(s) 459, 483, 700
Psp5II RGGWCCY 2 cut(s) 1390, 1625
Psp6I CCWGG 2 cut(s) 669, 920
PspCI CACGTG 2 cut(s) 582, 658
PspEI GGTNACC 1 cut(s) 1664
PspGI CCWGG 2 cut(s) 669, 920
PspN4I GGNNCC 8 cut(s) 757, 773, 903, 1279, 1392, 1455, 1551, 1627
PspPI GGNCC 9 cut(s) 361, 755, 1139, 1375, 1390, 1453, 1486, 1625, 1712
PspPPI RGGWCCY 2 cut(s) 1390, 1625
PspXI VCTCGAGB 1 cut(s) 452
PstNI CAGNNNCTG 1 cut(s) 1085
PsuI RGATCY 1 cut(s) 298
PsyI GACNNNGTC 1 cut(s) 730
PteI GCGCGC 1 cut(s) 1337
PvuII CAGCTG 1 cut(s) 1688
RsaI GTAC 3 cut(s) 578, 707, 1594
RsaNI GTAC 3 cut(s) 577, 706, 1593
SacI GAGCTC 3 cut(s) 459, 483, 700
SalI GTCGAC 1 cut(s) 724
SapI GCTCTTC 1 cut(s) 464
SaqAI TTAA 2 cut(s) 597, 702
SatI GCNGC 8 cut(s) 231, 559, 1134, 1174, 1204, 1305, 1437, 1440
Sau3AI GATC 5 cut(s) 298, 324, 796, 1070, 1447
Sau96I GGNCC 9 cut(s) 361, 755, 1139, 1375, 1390, 1453, 1486, 1625, 1712
SchI GAGTC 1 cut(s) 1768
ScrFI CCNGG 7 cut(s) 671, 693, 922, 1457, 1629, 1662, 1670
SduI GDGCHC 5 cut(s) 126, 459, 483, 700, 1554
SexAI ACCWGGT 1 cut(s) 920
SfcI CTRYAG 1 cut(s) 891
Sfr274I CTCGAG 1 cut(s) 452
SfuI TTCGAA 1 cut(s) 1634
SgrDI CGTCGACG 1 cut(s) 724
SinI GGWCC 5 cut(s) 361, 755, 1390, 1453, 1625
SlaI CTCGAG 1 cut(s) 452
SmlI CTYRAG 3 cut(s) 452, 680, 701
SmoI CTYRAG 3 cut(s) 452, 680, 701
SphI GCATGC 1 cut(s) 246
Sse9I AATT 6 cut(s) 178, 224, 609, 624, 907, 1250
SspI AATATT 1 cut(s) 1753
SspMI CTAG 1 cut(s) 1509
SstI GAGCTC 3 cut(s) 459, 483, 700
StyD4I CCNGG 7 cut(s) 669, 691, 920, 1455, 1627, 1660, 1668
StyI CCWWGG 3 cut(s) 852, 1111, 1378
TaaI ACNGT 8 cut(s) 340, 352, 405, 1024, 1081, 1282, 1516, 1708
TaqII GACCGA 1 cut(s) 13
TasI AATT 6 cut(s) 178, 224, 609, 624, 907, 1250
TatI WGTACW 1 cut(s) 576
TauI GCSGC 4 cut(s) 561, 1176, 1307, 1442
TfiI GAWTC 2 cut(s) 688, 1696
Tru1I TTAA 2 cut(s) 597, 702
Tru9I TTAA 2 cut(s) 597, 702
TscAI CASTG 3 cut(s) 610, 868, 1411
TseFI GTSAC 1 cut(s) 1651
TseI GCWGC 4 cut(s) 230, 1133, 1203, 1436
Tsp45I GTSAC 1 cut(s) 1651
TspDTI ATGAA 7 cut(s) 636, 777, 1093, 1226, 1279, 1538, 1753
TspGWI ACGGA 3 cut(s) 496, 805, 1482
TspRI CASTG 3 cut(s) 610, 868, 1411
Tth111I GACNNNGTC 1 cut(s) 730
Vha464I CTTAAG 1 cut(s) 701
VpaK11BI GGWCC 5 cut(s) 361, 755, 1390, 1453, 1625
XapI RAATTY 3 cut(s) 178, 624, 907
XceI RCATGY 2 cut(s) 246, 1369
XcmI CCANNNNNNNNNTGG 1 cut(s) 1042
XhoI CTCGAG 1 cut(s) 452
XmiI GTMKAC 1 cut(s) 725
XmnI GAANNNNTTC 1 cut(s) 1367
XspI CTAG 1 cut(s) 1509
ZraI GACGTC 1 cut(s) 729
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.