FvH4_2g29160
ERF Family

Probably involved in the RNA silencing pathway and required for the generation of small interfering RNAs (siRNAs)

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb2
OG0017992
Physical Location & Seq
Reverse (-)
22681587 .. 22685304
3718 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_2g29160.t1

Sequence Viewer

Length: 3312 bp
ATGGAATCACATCATCTCTTGAACTATGGCTCCCAACCAACACTAGGCCTAGGGTCTTGTTCAATGGAATCGATCCATCCACGTTACACCTACCTAAACAACTCTAGTTGTGCTATGGAGGATCCTTATTATTCTCTAGGGCTTTGTGAAACTGAAACCCTATTCAGGCTTTACGATGTGCGTGTCGAGATCGGAAGCCTTGTTCGGCAATACAAAGATGAGTTCTTGGTTGCCTGGGAAGGACCCCCTAAAGGAGTTGCCTTTACTGTGGACCCTTCTAGTAGGACTTGCAGGTTTTGCTTGACACAAAGCACCGCCCTATCCTATAGCGGTAGGAGAAACAAGCGTGTGAGGACCTACTGTGATTACAAAGTGGAGTTTTTGATTAGGGATATATGTGAGTACATTTGTTTTGAAAACAAGGAGATGGATTTTGTGATTCTGTTGAGGCTGTCTTCGTCGCCGTGTGTTTGGTGCAGGTCTCCTCATGAGAGTTGGAGTAGGACTACTGATTTCACTGCGAGTGGCGCCATTGGGAGGAGTAATGTCTACAAGTTTGTGATCCCTCGGCGCTATGTCGCTGAGTGGAACAAGGCCACTGATTATCTTAGACAACTGAGGGTGATGAAGTTTGATGTCAGATGGCCACTTGTGATTGAGAAGGAGCCGTATTTCGGGATGCCTAGTTCTAGTTTTTTCTACTCTATTGGTAACAAATTTGGAATTTGCTTCGAGATTATCTTCTTGGTTAATGCTGTAATGCACAAGGGAATTCTTAATCAGCATCAGTTGTCAGACTCAGACGGCTTCTTCGATTTACTGAGAAGCCAATCAAAGGATGTCAATGTGGCTGCATTGAACTACTTATACTCTTGTAGCCGTCATCCGGTGTTTGATGCCTGCCAGAGGCTGAGAGATCTGCAAGACTGGTTTGTGGCAAATCCTGAGCTTTTCAACCGTCCTAAAAGGTTGGATCATCTTGCTCATGTTAGGAGGTTGGTCATTACCCCAACCAGAGCCTATTGTATTCCACCAGAGGCTCAACTGTCCAATAGGGTTCTTAGAAAATACAAAGTCATAGGTGATCGGTTTTTAAGGGTTACTTTTATGGATGAAGGAATGCAGACTATTTACTCCAATTCTATGAATTCTTATGTGTATAATTCCTGCCCTCAGAGAACCAAAGTATCCAGAAGGGTTGAAGCCATTCTGAAAAATGGATTTGACTTTTGCGGCCGAAAGTACTCTTTCTTGGCCTTCTCATCCAATCAATTGAGGGAGCGTTCGGCCTGGTTTTTTTCTGAATGCAAGAGCACTACAATCCGCGACATCCAAACATGGATGGGGAAGTTCAAGAACAGAAATGTTGCAAAGTGTTCTGCTAGAATGGGTCTGTGCTTTTCATCTACATATGCAACGGTAGAAGTTCCATCAAGCAAGGTTAATCCAAGGCTCCAAGATATTGAGAGGAATGGATACAACTTTTCAGATGGGATTGGTAAGATTACCCCTGATCTTGCAATGAAAGTTGCAAAGAAACTGAACTTGGACGGTGATCCTCCATGTGCATACCAAATTAGATATGCTGGCTTTAAAGGGGTTATTGCTTGTTGGCCAAGCGAAGGGGATAGATTCCAGCTCTCTTTAAGGCGTAGCATGAACAAGTTCAATTCGCAACATACTACGCTGGAAATTTGTTCTTGGGCTAGGTTGCAGCCAGGTTTTTTAAACAGGCAGATTATAACATTGCTTTCGACACTGGATGTTCCAGATATGGTATTCCGGAGAATGCAGCAGCAAATGCTTTCCAACTTAAAACTGATGCTCATAGACATGGATGCTGCATATGATGTTGTCACTGCTTCATGTGGTGAACAAGGAAATGCAGCAGCCATAATGCTGAGTGCAGGTTTTACCCCGCACTCAGAACCTCATCTGCACGGTATGTTAACATGTATACGAGCCGCGCAGCTTTCTGGCCTCCGAGAAAAAGGCAGGATTTTTGTACCCTCGGGGAGATGGCTTATGGGCTGTCTAGATGAGCTAGGGGTACTTGAGCAAGGTCAGTGCTTTATCCAAGTGTCTAACCCGTCACTTGAAAACTGTTTTGCTAAGCATGGCTCTAGGTTTTCTCATGTAGAGAAAAACCTTCAAGTAATTAAAGGATTAGTTGTGGTAGCAAAGAATCCATGTCTTCATCCAGGCGATATAAGGATTCTCGAAGCAGTTGATGCCCCAGGTTTACACCATTTACATGATTGCCTTGTTTTTCCTCAGAAGGGTTGTAGGCCACACTCGAACGAAGCTTCTGGAAGTGATCTGGATGGGGACCTGTATTTTGTCACATGGGATGAAAATCTTATTCCCCCAAGTATGAAGAGCGAGGAAGCTATGGACTTTGATGCAGCAGAAGCCAAAAATTTGGACCGTCCTGTCACTAACCAGGACATTATAGAGTTTTTCGTGAGAAACATGGCGAATGAGAACCTGGGAGCAATATGCAATGCACATGTAGTTCATGCTGACAGGAGTGAAGATGGGGCTAAGGATGCCAACTGCATTAAACTAGCAAAGCTAGCGGCAATAGCAGTTGATATGCCTAAAACCGGGAAGATTGTTACAATGCCACCACACCTTAGACCAGAGTCGTACCCAGATTTCATGGGGAAAGAGGGCTTCAACTCTTACATGTCAACTAAAATCTTGGGAGAACTCTATCGCCAGATCACAGATGCCTATGATGAAGATATGGCAACCTCTGTCAAACTAGGTTGTACATATGATAGCCATTTGGTGGTTCCAGGAGCGCACAGTATCATCCCAGAAGCATTGGAGAGAAAGCGCTCTTACGATTTTCAGCTGAAGGCTCTAATGGGACAGTATAATGTCTTGAGGGAAGAAGAGATTGTTACTGGGTATATCTGGTCAATGCCGAAGAGCAGAAAGCGAGGGGAGCTCCAGGACCAGCTGAAGCAGGCTTACAGTGCTCTTAAGAAAGAGTTCAGGGAATGTTTTGAGAGGATTGATGGATATTTCATGTCAATGAGTGATGATCAGAAGAATATTATGTATGAGCAGAAGGCATCAGCTTGGTACATGGTAACTTATCACCCTAAGTTGGTGGAAAAATCACTCTATTTGCAAGGGCCAGATGGTCCTGGAGATGTGCCGATGTTAAGCTTTGCCTGGATTGCAACTGACTACCTTGCTCGGATCAAGATCAGGCGTAGTGGAGTGGAAAAGATCAACTCTAGAAAGCCAATCAACACTTTGAGAAGGTACCTCGCTGATAGGATAGGGACAGGAATCTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0000003 GO:0001172 GO:0002252 GO:0002376 GO:0003006 GO:0003674 GO:0003824 GO:0003968 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0006139 GO:0006396 GO:0006725 GO:0006807 GO:0006950 GO:0006952 GO:0006955 GO:0007154 GO:0007275 GO:0008037 GO:0008150 GO:0008152 GO:0009058 GO:0009059 GO:0009605 GO:0009607 GO:0009615 GO:0009616 GO:0009791 GO:0009856 GO:0009875 GO:0009888 GO:0009892 GO:0009908 GO:0009987 GO:0010033 GO:0010073 GO:0010074 GO:0010267 GO:0010467 GO:0010468 GO:0010492 GO:0010605 GO:0010608 GO:0010629 GO:0014070 GO:0016070 GO:0016246 GO:0016441 GO:0016458 GO:0016740 GO:0016772 GO:0016779 GO:0018130 GO:0019222 GO:0019438 GO:0019827 GO:0022414 GO:0030422 GO:0031047 GO:0031050 GO:0032501 GO:0032502 GO:0032774 GO:0034062 GO:0034641 GO:0034654 GO:0035194 GO:0035821 GO:0040029 GO:0042221 GO:0043170 GO:0043207 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043331 GO:0044003 GO:0044237 GO:0044238 GO:0044249 GO:0044271 GO:0044403 GO:0044419 GO:0044424 GO:0044464 GO:0044706 GO:0045087 GO:0046483 GO:0048366 GO:0048367 GO:0048437 GO:0048438 GO:0048440 GO:0048467 GO:0048507 GO:0048519 GO:0048544 GO:0048608 GO:0048731 GO:0048827 GO:0048856 GO:0050789 GO:0050896 GO:0051607 GO:0051701 GO:0051704 GO:0051707 GO:0051716 GO:0051817 GO:0052018 GO:0052249 GO:0060255 GO:0061458 GO:0065007 GO:0070887 GO:0070918 GO:0071310 GO:0071359 GO:0071407 GO:0071704 GO:0090304 GO:0090567 GO:0097659 GO:0097747 GO:0098542 GO:0098586 GO:0098727 GO:0099402 GO:0140098 GO:1901360 GO:1901362 GO:1901576 GO:1901698 GO:1901699
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
RdRP

Protein Analysis

1104

Amino Acids

125.87

Weight (kDa)

8.65

Isoelectric Point (pI)

47.12

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
RDR6_2nd PF24577 56 - 208 2.8e-43 RNA-dependent RNA polymerase 6 second domain
RdRP_helical PF26252 236 - 317 9e-15 RNA dependent RNA polymerase, helical domain
RdRP PF05183 335 - 909 2e-187 RNA dependent RNA polymerase
RdRP_head PF26253 926 - 1089 2.1e-37 RdRP, head domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
Loading...
Style Settings
Image
Tree File
Tip: Beautify your tree with professional tools

Download the Full Tree (.nwk) file, then upload it to any of the following tools to customize colors, fonts, annotations, clades, and branch support.

Publication-ready
iTOL Interactive Tree Of Life · Most popular EvolView Flexible annotations MEGA Desktop phylogenetics suite FigTree Desktop tree editor

Orthologous Genes (Group: OG0017992)

Species Orthologous Gene IDs
fragaria_vesca FvH4_2g29160
rosa_chinensis RchiOBHm_Chr6g0298021
rosa_laevigata RLG00000011542
rosa_multiflora Rmu_co8512357.1_g000001 Rmu_sc0002800.1_g000021
rosa_rugosa Rorug06G0278100
rosa_samantha Rh6AG388400 Rh6BG396600 Rh6CG402600 Rh6DG388800
rosa_wichuraiana Rw6G033900

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 1742
AasI GACNNNNNNGTC 1 cut(s) 2432
Acc36I ACCTGC 3 cut(s) 282, 468, 1898
Acc65I GGTACC 1 cut(s) 3279
AccB1I GGYRCC 2 cut(s) 527, 3279
AccB7I CCANNNNNTGG 1 cut(s) 2794
AccI GTMKAC 2 cut(s) 549, 1957
AccII CGCG 2 cut(s) 1326, 1967
AccIII TCCGGA 1 cut(s) 1782
AciI CCGC 7 cut(s) 315, 330, 1233, 1324, 1919, 1965, 2579
AclWI GGATC 7 cut(s) 67, 116, 129, 556, 981, 1550, 3221
AcoI YGGCCR 3 cut(s) 644, 1234, 1613
AcsI RAATTY 6 cut(s) 716, 723, 771, 1147, 1692, 2419
AcuI CTGAAG 2 cut(s) 2882, 2990
AcyI GRCGYC 1 cut(s) 528
AfaI GTAC 8 cut(s) 404, 1244, 2007, 2052, 2651, 2776, 3095, 3281
AfeI AGCGCT 1 cut(s) 2843
AflII CTTAAG 1 cut(s) 2990
AflIII ACRYGT 3 cut(s) 1952, 2509, 2688
AjuI GAANNNNNNNTTGG 2 cut(s) 1529, 1561
AloI GAACNNNNNNTCC 6 cut(s) 14, 46, 186, 218, 670, 702
Alw21I GWGCWC 3 cut(s) 1316, 2958, 2989
Alw26I GTCTC 1 cut(s) 486
AlwI GGATC 7 cut(s) 67, 116, 129, 556, 981, 1550, 3221
AlwNI CAGNNNCTG 1 cut(s) 910
Ama87I CYCGRG 1 cut(s) 2011
Aor13HI TCCGGA 1 cut(s) 1782
Aor51HI AGCGCT 1 cut(s) 2843
ApeKI GCWGC 9 cut(s) 851, 1714, 1792, 1795, 1841, 1886, 1889, 1969, 2405
ApoI RAATTY 6 cut(s) 716, 723, 771, 1147, 1692, 2419
ArsI GACNNNNNNTTYG 4 cut(s) 794, 826, 2321, 2353
Asp700I GAANNNNTTC 3 cut(s) 1206, 1664, 2999
Asp718I GGTACC 1 cut(s) 3279
AspA2I CCTAGG 1 cut(s) 49
AspLEI GCGC 5 cut(s) 530, 573, 1969, 2809, 2844
AspS9I GGNCC 8 cut(s) 242, 271, 354, 2329, 2425, 2962, 3146, 3155
AsuC2I CCSGG 1 cut(s) 2608
AsuHPI GGTGA 5 cut(s) 634, 1094, 1565, 1883, 3101
AsuNHI GCTAGC 1 cut(s) 2575
AvaI CYCGRG 1 cut(s) 2011
AvaII GGWCC 7 cut(s) 242, 271, 354, 2329, 2425, 2962, 3155
AvrII CCTAGG 1 cut(s) 49
BaeI ACNNNNGTAYC 2 cut(s) 1170, 1203
BalI TGGCCA 2 cut(s) 646, 1615
BamHI GGATCC 1 cut(s) 121
BanI GGYRCC 2 cut(s) 527, 3279
BanII GRGCYC 1 cut(s) 2958
BarI GAAGNNNNNNTAC 2 cut(s) 2963, 2995
BbsI GAAGAC 2 cut(s) 447, 2186
Bbv12I GWGCWC 3 cut(s) 1316, 2958, 2989
BbvI GCAGC 9 cut(s) 838, 1726, 1804, 1807, 1828, 1898, 1901, 1981, 2417
BceAI ACGGC 4 cut(s) 448, 652, 820, 864
BciVI GTATCC 2 cut(s) 1198, 1469
BclI TGATCA 1 cut(s) 3052
BcnI CCSGG 1 cut(s) 2608
BcoDI GTCTC 1 cut(s) 486
BfmI CTRYAG 1 cut(s) 325
BfoI RGCGCY 3 cut(s) 531, 574, 2845
BfrI CTTAAG 1 cut(s) 2990
BfuAI ACCTGC 3 cut(s) 282, 468, 1898
BfuI GTATCC 2 cut(s) 1198, 1469
BglII AGATCT 1 cut(s) 916
BlnI CCTAGG 1 cut(s) 49
BlpI GCTNAGC 1 cut(s) 2112
BmcAI AGTACT 1 cut(s) 1244
Bme18I GGWCC 7 cut(s) 242, 271, 354, 2329, 2425, 2962, 3155
BmeT110I CYCGRG 1 cut(s) 2011
BmgT120I GGNCC 8 cut(s) 242, 271, 354, 2329, 2425, 2962, 3146, 3155
BmrI ACTGGG 1 cut(s) 2922
BmtI GCTAGC 1 cut(s) 2579
BmuI ACTGGG 1 cut(s) 2922
BoxI GACNNNNGTC 1 cut(s) 2644
BpiI GAAGAC 2 cut(s) 447, 2186
BplI GAGNNNNNCTC 2 cut(s) 2940, 2972
BpmI CTGGAG 2 cut(s) 2942, 3180
Bpu10I CCTNAGC 2 cut(s) 945, 2544
Bpu1102I GCTNAGC 1 cut(s) 2112
BpuEI CTTGAG 2 cut(s) 2075, 2911
BpuMI CCSGG 1 cut(s) 2608
Bsa29I ATCGAT 1 cut(s) 71
BsaBI GATNNNNATC 2 cut(s) 2355, 3218
BsaHI GRCGYC 1 cut(s) 528
BsaI GGTCTC 1 cut(s) 486
BsaJI CCNNGG 7 cut(s) 49, 234, 566, 1448, 2010, 2236, 2488
BsaWI WCCGGW 2 cut(s) 886, 1782
BsaXI ACNNNNNCTCC 2 cut(s) 14, 44
Bse1I ACTGG 3 cut(s) 932, 1764, 2917
Bse3DI GCAATG 3 cut(s) 1527, 1745, 2509
Bse8I GATNNNNATC 2 cut(s) 2355, 3218
BseAI TCCGGA 1 cut(s) 1782
BseCI ATCGAT 1 cut(s) 71
BseDI CCNNGG 7 cut(s) 49, 234, 566, 1448, 2010, 2236, 2488
BseJI GATNNNNATC 2 cut(s) 2355, 3218
BseMI GCAATG 3 cut(s) 1527, 1745, 2509
BseNI ACTGG 3 cut(s) 932, 1764, 2917
BseRI GAGGAG 2 cut(s) 474, 553
BseX3I CGGCCG 1 cut(s) 1234
BseXI GCAGC 9 cut(s) 838, 1726, 1804, 1807, 1828, 1898, 1901, 1981, 2417
BsgI GTGCAG 3 cut(s) 496, 1922, 1926
Bsh1236I CGCG 2 cut(s) 1326, 1967
Bsh1285I CGRYCG 1 cut(s) 1237
BshNI GGYRCC 2 cut(s) 527, 3279
BshVI ATCGAT 1 cut(s) 71
BsiEI CGRYCG 1 cut(s) 1237
BsiHKAI GWGCWC 3 cut(s) 1316, 2958, 2989
BsiHKCI CYCGRG 1 cut(s) 2011
BsiSI CCGG 3 cut(s) 887, 1783, 2607
BslFI GGGAC 2 cut(s) 2342, 2889
BsmAI GTCTC 1 cut(s) 486
BsmFI GGGAC 2 cut(s) 2342, 2889
BsmI GAATGC 3 cut(s) 1125, 1310, 1794
Bso31I GGTCTC 1 cut(s) 486
BsoBI CYCGRG 1 cut(s) 2011
Bsp1286I GDGCHC 3 cut(s) 1316, 2958, 2989
Bsp13I TCCGGA 1 cut(s) 1782
Bsp1407I TGTACA 1 cut(s) 2774
Bsp1720I GCTNAGC 1 cut(s) 2112
BspACI CCGC 7 cut(s) 315, 330, 1233, 1324, 1919, 1965, 2579
BspDI ATCGAT 1 cut(s) 71
BspEI TCCGGA 1 cut(s) 1782
BspFNI CGCG 2 cut(s) 1326, 1967
BspHI TCATGA 1 cut(s) 487
BspMI ACCTGC 3 cut(s) 282, 468, 1898
BspOI GCTAGC 1 cut(s) 2579
BspPI GGATC 7 cut(s) 67, 116, 129, 556, 981, 1550, 3221
BspQI GCTCTTC 2 cut(s) 2372, 2930
BspT107I GGYRCC 2 cut(s) 527, 3279
BspTI CTTAAG 1 cut(s) 2990
BspTNI GGTCTC 1 cut(s) 486
BsrDI GCAATG 3 cut(s) 1527, 1745, 2509
BsrGI TGTACA 1 cut(s) 2774
BsrI ACTGG 3 cut(s) 932, 1764, 2917
BssECI CCNNGG 7 cut(s) 49, 234, 566, 1448, 2010, 2236, 2488
BssNAI GTATAC 1 cut(s) 1958
BssNI GRCGYC 1 cut(s) 528
BssT1I CCWWGG 2 cut(s) 49, 1448
Bst1107I GTATAC 1 cut(s) 1958
Bst6I CTCTTC 3 cut(s) 2372, 2895, 2930
BstACI GRCGYC 1 cut(s) 528
BstAFI CTTAAG 1 cut(s) 2990
BstAPI GCANNNNNTGC 3 cut(s) 297, 1801, 2231
BstAUI TGTACA 1 cut(s) 2774
BstC8I GCNNGC 4 cut(s) 901, 1588, 2577, 2976
BstENI CCTNNNNNAGG 1 cut(s) 904
BstFNI CGCG 2 cut(s) 1326, 1967
BstH2I RGCGCY 3 cut(s) 531, 574, 2845
BstHHI GCGC 5 cut(s) 530, 573, 1969, 2809, 2844
BstMAI GTCTC 1 cut(s) 486
BstMCI CGRYCG 1 cut(s) 1237
BstNSI RCATGY 3 cut(s) 1956, 2513, 2692
BstPAI GACNNNNGTC 1 cut(s) 2644
BstSFI CTRYAG 1 cut(s) 325
BstUI CGCG 2 cut(s) 1326, 1967
BstV1I GCAGC 9 cut(s) 838, 1726, 1804, 1807, 1828, 1898, 1901, 1981, 2417
BstV2I GAAGAC 2 cut(s) 447, 2186
BstX2I RGATCY 2 cut(s) 121, 916
BstXI CCANNNNNNTGG 1 cut(s) 2422
BstYI RGATCY 2 cut(s) 121, 916
BstZ17I GTATAC 1 cut(s) 1958
BstZI CGGCCG 1 cut(s) 1234
Bsu15I ATCGAT 1 cut(s) 71
BsuI GTATCC 2 cut(s) 1198, 1469
BsuTUI ATCGAT 1 cut(s) 71
BtsI GCAGTG 2 cut(s) 516, 1857
BtsIMutI CAGTG 6 cut(s) 516, 597, 1757, 1857, 2072, 2989
BveI ACCTGC 3 cut(s) 282, 468, 1898
Cac8I GCNNGC 4 cut(s) 901, 1588, 2577, 2976
CaiI CAGNNNCTG 1 cut(s) 910
CciI TCATGA 1 cut(s) 487
CfoI GCGC 5 cut(s) 530, 573, 1969, 2809, 2844
Cfr13I GGNCC 8 cut(s) 242, 271, 354, 2329, 2425, 2962, 3146, 3155
ClaI ATCGAT 1 cut(s) 71
Csp6I GTAC 8 cut(s) 403, 1243, 2006, 2051, 2650, 2775, 3094, 3280
CviQI GTAC 8 cut(s) 403, 1243, 2006, 2051, 2650, 2775, 3094, 3280
DinI GGCGCC 1 cut(s) 529
DraI TTTAAA 2 cut(s) 1594, 1728
DrdI GACNNNNNNGTC 1 cut(s) 2432
DseDI GACNNNNNNGTC 1 cut(s) 2432
EaeI YGGCCR 3 cut(s) 644, 1234, 1613
EagI CGGCCG 1 cut(s) 1234
Eam1104I CTCTTC 3 cut(s) 2372, 2895, 2930
EarI CTCTTC 3 cut(s) 2372, 2895, 2930
Ecl136II GAGCTC 1 cut(s) 2956
EclXI CGGCCG 1 cut(s) 1234
Eco130I CCWWGG 2 cut(s) 49, 1448
Eco147I AGGCCT 1 cut(s) 48
Eco24I GRGCYC 1 cut(s) 2958
Eco31I GGTCTC 1 cut(s) 486
Eco47I GGWCC 7 cut(s) 242, 271, 354, 2329, 2425, 2962, 3155
Eco47III AGCGCT 1 cut(s) 2843
Eco52I CGGCCG 1 cut(s) 1234
Eco53kI GAGCTC 1 cut(s) 2956
Eco57I CTGAAG 2 cut(s) 2882, 2990
Eco88I CYCGRG 1 cut(s) 2011
EcoICRI GAGCTC 1 cut(s) 2956
EcoNI CCTNNNNNAGG 1 cut(s) 904
EcoO109I RGGNCCY 3 cut(s) 242, 354, 2329
EcoRI GAATTC 2 cut(s) 771, 1147
EcoT14I CCWWGG 2 cut(s) 49, 1448
EcoT38I GRGCYC 1 cut(s) 2958
EgeI GGCGCC 1 cut(s) 529
EheI GGCGCC 1 cut(s) 529
ErhI CCWWGG 2 cut(s) 49, 1448
FalI AAGNNNNNCTT 2 cut(s) 1087, 1119
FaqI GGGAC 2 cut(s) 2342, 2889
FauI CCCGC 1 cut(s) 1926
FauNDI CATATG 3 cut(s) 1411, 1846, 2779
FbaI TGATCA 1 cut(s) 3052
FblI GTMKAC 2 cut(s) 549, 1957
FriOI GRGCYC 1 cut(s) 2958
GlaI GCGC 5 cut(s) 529, 572, 1968, 2808, 2843
GsuI CTGGAG 2 cut(s) 2942, 3180
HaeII RGCGCY 3 cut(s) 531, 574, 2845
HapII CCGG 3 cut(s) 887, 1783, 2607
HhaI GCGC 5 cut(s) 530, 573, 1969, 2809, 2844
Hin1I GRCGYC 1 cut(s) 528
Hin6I GCGC 5 cut(s) 528, 571, 1967, 2807, 2842
HinP1I GCGC 5 cut(s) 528, 571, 1967, 2807, 2842
HincII GTYRAC 2 cut(s) 1950, 2694
HindII GTYRAC 2 cut(s) 1950, 2694
HindIII AAGCTT 2 cut(s) 2304, 3178
HinfI GANTC 9 cut(s) 5, 68, 439, 797, 1632, 2185, 2215, 2645, 3306
HpaI GTTAAC 1 cut(s) 1950
HpaII CCGG 3 cut(s) 887, 1783, 2607
HphI GGTGA 5 cut(s) 634, 1094, 1565, 1883, 3101
Hpy166II GTNNAC 7 cut(s) 271, 550, 1874, 1950, 1958, 2243, 2694
Hpy8I GTNNAC 7 cut(s) 271, 550, 1874, 1950, 1958, 2243, 2694
Hpy99I CGWCG 1 cut(s) 463
HpyCH4IV ACGT 1 cut(s) 82
HpySE526I ACGT 1 cut(s) 82
Hsp92I GRCGYC 1 cut(s) 528
HspAI GCGC 5 cut(s) 528, 571, 1967, 2807, 2842
KasI GGCGCC 1 cut(s) 527
Kpn2I TCCGGA 1 cut(s) 1782
KpnI GGTACC 1 cut(s) 3283
Ksp22I TGATCA 1 cut(s) 3052
KspAI GTTAAC 1 cut(s) 1950
LguI GCTCTTC 2 cut(s) 2372, 2930
LmnI GCTCC 8 cut(s) 35, 664, 1279, 1458, 2492, 2804, 2953, 2961
Lsp1109I GCAGC 9 cut(s) 838, 1726, 1804, 1807, 1828, 1898, 1901, 1981, 2417
MaeII ACGT 1 cut(s) 82
MfeI CAATTG 1 cut(s) 1271
MflI RGATCY 2 cut(s) 121, 916
MhlI GDGCHC 3 cut(s) 1316, 2958, 2989
MlsI TGGCCA 2 cut(s) 646, 1615
MluNI TGGCCA 2 cut(s) 646, 1615
Mly113I GGCGCC 1 cut(s) 528
MlyI GAGTC 2 cut(s) 791, 2654
MmeI TCCRAC 3 cut(s) 476, 951, 1833
Mox20I TGGCCA 2 cut(s) 646, 1615
MroI TCCGGA 1 cut(s) 1782
MroXI GAANNNNTTC 3 cut(s) 1206, 1664, 2999
MscI TGGCCA 2 cut(s) 646, 1615
MslI CAYNNNNRTG 3 cut(s) 1832, 2253, 3041
Msp20I TGGCCA 2 cut(s) 646, 1615
MspA1I CMGCKG 2 cut(s) 2860, 2968
MspCI CTTAAG 1 cut(s) 2990
MspI CCGG 3 cut(s) 887, 1783, 2607
MunI CAATTG 1 cut(s) 1271
Mva1269I GAATGC 3 cut(s) 1125, 1310, 1794
MvnI CGCG 2 cut(s) 1326, 1967
NarI GGCGCC 1 cut(s) 528
NciI CCSGG 1 cut(s) 2608
NdeI CATATG 3 cut(s) 1411, 1846, 2779
NheI GCTAGC 1 cut(s) 2575
NmeAIII GCCGAG 1 cut(s) 547
NmuCI GTSAC 4 cut(s) 1855, 2091, 2341, 2434
NspI RCATGY 3 cut(s) 1956, 2513, 2692
PagI TCATGA 1 cut(s) 487
PceI AGGCCT 1 cut(s) 48
PciI ACATGT 3 cut(s) 1952, 2509, 2688
PciSI GCTCTTC 2 cut(s) 2372, 2930
PcsI WCGNNNNNNNCGW 1 cut(s) 810
PctI GAATGC 3 cut(s) 1125, 1310, 1794
PdmI GAANNNNTTC 3 cut(s) 1206, 1664, 2999
PfeI GAWTC 7 cut(s) 5, 68, 439, 1632, 2185, 2215, 3306
PflMI CCANNNNNTGG 1 cut(s) 2794
PfoI TCCNGGA 3 cut(s) 2800, 2958, 3157
PleI GAGTC 2 cut(s) 791, 2653
PluTI GGCGCC 1 cut(s) 531
PpsI GAGTC 2 cut(s) 791, 2653
PpuMI RGGWCCY 3 cut(s) 242, 354, 2329
PscI ACATGT 3 cut(s) 1952, 2509, 2688
PshAI GACNNNNGTC 1 cut(s) 2644
PsiI TTATAA 1 cut(s) 1742
Psp124BI GAGCTC 1 cut(s) 2958
Psp5II RGGWCCY 3 cut(s) 242, 354, 2329
PspPI GGNCC 8 cut(s) 242, 271, 354, 2329, 2425, 2962, 3146, 3155
PspPPI RGGWCCY 3 cut(s) 242, 354, 2329
PsrI GAACNNNNNNTAC 2 cut(s) 851, 883
PstNI CAGNNNCTG 1 cut(s) 910
PsuI RGATCY 2 cut(s) 121, 916
PvuII CAGCTG 2 cut(s) 2860, 2968
RsaI GTAC 8 cut(s) 404, 1244, 2007, 2052, 2651, 2776, 3095, 3281
RsaNI GTAC 8 cut(s) 403, 1243, 2006, 2051, 2650, 2775, 3094, 3280
RseI CAYNNNNRTG 3 cut(s) 1832, 2253, 3041
SacI GAGCTC 1 cut(s) 2958
SapI GCTCTTC 2 cut(s) 2372, 2930
Sau96I GGNCC 8 cut(s) 242, 271, 354, 2329, 2425, 2962, 3146, 3155
ScaI AGTACT 1 cut(s) 1244
SchI GAGTC 2 cut(s) 791, 2654
SduI GDGCHC 3 cut(s) 1316, 2958, 2989
SfcI CTRYAG 1 cut(s) 325
SfoI GGCGCC 1 cut(s) 529
SinI GGWCC 7 cut(s) 242, 271, 354, 2329, 2425, 2962, 3155
SmiMI CAYNNNNRTG 3 cut(s) 1832, 2253, 3041
SmlI CTYRAG 3 cut(s) 2054, 2890, 2990
SmoI CTYRAG 3 cut(s) 2054, 2890, 2990
SseBI AGGCCT 1 cut(s) 48
SsiI CCGC 7 cut(s) 315, 330, 1233, 1324, 1919, 1965, 2579
SspDI GGCGCC 1 cut(s) 527
SspI AATATT 1 cut(s) 3064
SstI GAGCTC 1 cut(s) 2958
StuI AGGCCT 1 cut(s) 48
StyI CCWWGG 2 cut(s) 49, 1448
TaiI ACGT 1 cut(s) 85
TaqI TCGA 7 cut(s) 71, 186, 732, 813, 1754, 2220, 2297
TatI WGTACW 3 cut(s) 402, 1242, 2774
TauI GCSGC 3 cut(s) 1236, 1967, 2582
TfiI GAWTC 7 cut(s) 5, 68, 439, 1632, 2185, 2215, 3306
TscAI CASTG 6 cut(s) 523, 604, 1764, 1864, 2072, 2989
TseFI GTSAC 4 cut(s) 1855, 2091, 2341, 2434
TseI GCWGC 9 cut(s) 851, 1714, 1792, 1795, 1841, 1886, 1889, 1969, 2405
Tsp45I GTSAC 4 cut(s) 1855, 2091, 2341, 2434
TspRI CASTG 6 cut(s) 523, 604, 1764, 1864, 2072, 2989
Van91I CCANNNNNTGG 1 cut(s) 2794
Vha464I CTTAAG 1 cut(s) 2990
VpaK11BI GGWCC 7 cut(s) 242, 271, 354, 2329, 2425, 2962, 3155
XagI CCTNNNNNAGG 1 cut(s) 904
XapI RAATTY 6 cut(s) 716, 723, 771, 1147, 1692, 2419
XbaI TCTAGA 2 cut(s) 2035, 3251
XceI RCATGY 3 cut(s) 1956, 2513, 2692
XmaJI CCTAGG 1 cut(s) 49
XmiI GTMKAC 2 cut(s) 549, 1957
XmnI GAANNNNTTC 3 cut(s) 1206, 1664, 2999
ZrmI AGTACT 1 cut(s) 1244
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.