Rw6G033900
ERF Family

Probably involved in the RNA silencing pathway and required for the generation of small interfering RNAs (siRNAs)

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_wichuraiana
Chr6
OG0017992
Physical Location & Seq
Reverse (-)
58468556 .. 58471873
3318 bp
Loading structure...
UTR
Exon/CDS
Intron
Rw6G033900.1

Sequence Viewer

Length: 3267 bp
ATGGAATCACATCGATTCTTGAACCATGGCTCTCCACCAATACTAGGGTCGTGTTCAATGGAGTCGATCAATCCACACTACACATACCTAAACAACACGAGTTGTGCTATGGAGGATTCTTATTCTCTAGGGCGTTGTGAAACTGAAACCCTATTCAGGCTTTACGATGTGCGTGTCGAGATCGGAACCCTAGTTCGCCAAGACCAAGATGAGTTCTTGGTTGCTTGGGAAGGACCCCCTAAAGGAGTTGCCTTTACTGTGGACCCTTCTACTAGGACTTGCAGGTTTTGCTTGACACAAAACACTGCCTTTTCGTATGGTGGTAGGAGAAACAAGCGTGTGAGGACGTACTGTGATTATAAAGTGGAGTTTTTGGTTAGGGACATCAGTGAGTTCAAGCATTACGAAACCTTGGAGATGGATTTTGTGATTCTGTTGAGGCTGGCTTGGTCGCCATGTGTTTCGTGTAGGTCTCCTCATGATGAGAGTTGGATCAGGACTACTGATTTCACTGCAAATGGCGCCATTGGAAGGAGTAATTTCTACAAGTTTTCGGTCCCTCGGTACTATGGGGCCGAGTGGAACAAGGCCATTGATTATCTTAGACAGCTGAGGGTGCTGGAGTTAGATATAAAATGGCCACTTGGGATTCAGAAGGAGCCCTTTTTCGGGATGCCTAGTTCCGATCCTTTCTACTCTATTGGTCACAAACATGCAATTTGCTTCGAGATTATCTTCTTGGTTAATGCGCTAATGCACAAGGGAATTTTCAATCAGCATCAGTTGTCAGACTGCTTCTTCGATTTACTGAGAAGCCAATCAAAGGATGTCAATGTGGCTGCATTGAACTACTTATATTCGTGCAGCCGGCGCCCGGTGTTAGATGCCTGTCAGAGGCTGAGAGCTGTGCAAGACTGGTTTGTGGCAAATCCCGAGCTTTTCGAGAGTCCTAAAAGGTTGGATGATATTGCTCATGTCAGGAGGTTGGTCATTACCCCAACTAAAGCCTATTGTATTCCACCAGAGGCTCAACTGTCAAATAGGGTTCTTAGAAAATACAAACAAATAGGTGATCGGTTTCTAAGGGTTACTTTTATGGATGAAGGGATGCAGACTATTTATTCCAATGCTATGAATTCTTATGGGGAAAAATCCTGCCCTCAGAGAACAAAAGTATTTGGAAGGGTTGAGGCCATTCTAAAAAATGGATTTGACTTATGCGGCCGAAGGTACTCTTTCTTGGCCTTCTCATCCAATCAATTGAGGGAGCGCTCAGCCTGGTTTTTTGCTGAATGCAAGAGCATTACGATCCACGATATCCAAACTTGGATGGGGAAGTTCACCAACAGAAATGTTGCAAAATATTCTGCTAGAATGGGTCAATGCTTTTCGTCTACATATGCAACTGTAGAAGTTCCATCAAGCAAGGTTAATCCTAAGCTTCGCGATATTGAGAGGAATGGATACAATTTTTCAGATGGGATTGGTAAGATTTCCCCTGCTCTTGCGATGGAAGTTGCAAAGAAACTGAAATTGGACTGCAGTGATCCTCCTTGTGCATACCAAATTAGATATGCTGGCTTTAAAGGGGTTATTGCTTGTTGGCCAAGCGAAGGGGATAGATTCCAGCTCTCTTTAAGGCCTAGCATGAACAAGTTTGAGTCGCACCATACTACATTGGAAATTTGTTCTTGGACTAGGCTGCAGCCGGGGTTTTTAAACAGGCAGATTATAACATTGCTTTCAACACTGCATGTTAAAGATATGGTATTCTGGAGAATGCAGCAGCAAATGGTTTCCAACTTAAAACAGATGCTCACAGACATGGATGCCGCATTTGATGTTGTCACTGCCTCATGCGGTGAACAAGGAAATGCGGCAGCTATAATGCTGAGTGCAGGTTTTAATCCGCAGACTGAACCTCATTTGCGCGGCATGTTAACATGTATACAAGCCGCGCAGCTTTGGGGCCTCCGAGAAAAGTCTAGGATTTTTGTTCCCTCGGGGAGATGGCTTATGGGATGTCTAGATGAGCTAGGGGTACTTGAACAAGGTCAGTGCTTTATCCAAGTGTCTAACCCATCACTTGAAAACTGTTTTGCAAAGCATGGCTCTAGGTTTTGCCCGATAGAGAAAAACCTACAAGTAATAAAAGGATTAGTTATGGTAGCAAAGAATCCATGTCTTCATCCAGGTGATATAAGGATTCTTGAAGCAGTAGATGCCCCAGGTTTACACCACTTACATGATTGCCTTGTTTTCCCTCAGAAGGGTTATAGGCCACACTCGAACGAAGCTTCTGGAAGTGACCTGGATGGGGACCTGTACTTTGTCACATGGGATGAAAATCTTATTCCCCCTAGTAAGAAGAGCGAGAAACCTATGGAGTATGATGCTGCAGAAGCCAAAACTTTGGACCGTCCTAACCTGGGGGCGATATGCAATGCACATGTAGTTCATGCTGACATCAGTGAACATGGGGCTTCGGATGACAACTGCATCAAACTAGCGAAGCTAGCAGCAATAGCAGTTGATCTACCAAAAACTGGGAAGATTGTTCCAATGCCATCGTACCTAAAACCAGAGTTGTACCCAGATTTCATGGGGAAAGAGGGCTTCAAGTCTTACATGTCAACTAAAATCTTGGGGCTACTCTATCGCCAGATCACAGACGCCTATGATGAAGATCTGGCAACCTCTTTTAACCTAGGTTATGGTCCAACTGACATGCCATATGATATTGATTTGGAGGTTCCAGGAGCCCACAATATCGTAGTAGAAGCATTGGAGAGAAAGCGCTCTTATGATTTTCAGCTGAAGGGTCTAATGGGACAATATAATGTTGTGAGGGAAGAAGAGATTGTCACTGGGTATGTTTGGTCAATGCCGAAGAGCAGGAAGCGAGGGGAGCTCCAGGAGCAGCTGAAACAGTCTTACAGTGCTCTGAAGAAAGAGTTCAGGCAATGTTTTGAGAGAATTGATGGAGATTTCAAGTCGCTGAGTAATGAGCAGAAGAATTTTTTGTATGAGCAGAAGGCATCAGCTTGGTACATGGTAACTTATCATCCTAAGTTGGTGAAAGAATCACTCTATTTGCAAGGGCCAGATGGTCCTGGAGATGTGCCGATGTTGAGCTTTGCCTGGATTGCAACTGACTACCTTGCTCGGATCAAGATCAAGCGTTGTGGAGTGGGAATGATCGACTCTAGAAAGCCAATCAACTCTTTGAGAAGGTACCTTGCGGATAGGATACGGACAGCCATTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0000003 GO:0001172 GO:0002252 GO:0002376 GO:0003006 GO:0003674 GO:0003824 GO:0003968 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0006139 GO:0006396 GO:0006725 GO:0006807 GO:0006950 GO:0006952 GO:0006955 GO:0007154 GO:0007275 GO:0008037 GO:0008150 GO:0008152 GO:0009058 GO:0009059 GO:0009605 GO:0009607 GO:0009615 GO:0009616 GO:0009791 GO:0009856 GO:0009875 GO:0009888 GO:0009892 GO:0009908 GO:0009987 GO:0010033 GO:0010073 GO:0010074 GO:0010267 GO:0010467 GO:0010468 GO:0010492 GO:0010605 GO:0010608 GO:0010629 GO:0014070 GO:0016070 GO:0016246 GO:0016441 GO:0016458 GO:0016740 GO:0016772 GO:0016779 GO:0018130 GO:0019222 GO:0019438 GO:0019827 GO:0022414 GO:0030422 GO:0031047 GO:0031050 GO:0032501 GO:0032502 GO:0032774 GO:0034062 GO:0034641 GO:0034654 GO:0035194 GO:0035821 GO:0040029 GO:0042221 GO:0043170 GO:0043207 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043331 GO:0044003 GO:0044237 GO:0044238 GO:0044249 GO:0044271 GO:0044403 GO:0044419 GO:0044424 GO:0044464 GO:0044706 GO:0045087 GO:0046483 GO:0048366 GO:0048367 GO:0048437 GO:0048438 GO:0048440 GO:0048467 GO:0048507 GO:0048519 GO:0048544 GO:0048608 GO:0048731 GO:0048827 GO:0048856 GO:0050789 GO:0050896 GO:0051607 GO:0051701 GO:0051704 GO:0051707 GO:0051716 GO:0051817 GO:0052018 GO:0052249 GO:0060255 GO:0061458 GO:0065007 GO:0070887 GO:0070918 GO:0071310 GO:0071359 GO:0071407 GO:0071704 GO:0090304 GO:0090567 GO:0097659 GO:0097747 GO:0098542 GO:0098586 GO:0098727 GO:0099402 GO:0140098 GO:1901360 GO:1901362 GO:1901576 GO:1901698 GO:1901699
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
RdRP

Protein Analysis

1088

Amino Acids

124.17

Weight (kDa)

8.44

Isoelectric Point (pI)

43.15

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
RDR6_2nd PF24577 53 - 206 9.1e-50 RNA-dependent RNA polymerase 6 second domain
RdRP_helical PF26252 232 - 313 1.1e-15 RNA dependent RNA polymerase, helical domain
RdRP PF05183 331 - 889 1.4e-177 RNA dependent RNA polymerase
RdRP_head PF26253 911 - 1061 6.7e-37 RdRP, head domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
Loading...
Style Settings
Image
Tree File
Tip: Beautify your tree with professional tools

Download the Full Tree (.nwk) file, then upload it to any of the following tools to customize colors, fonts, annotations, clades, and branch support.

Publication-ready
iTOL Interactive Tree Of Life · Most popular EvolView Flexible annotations MEGA Desktop phylogenetics suite FigTree Desktop tree editor

Orthologous Genes (Group: OG0017992)

Species Orthologous Gene IDs
fragaria_vesca FvH4_2g29160
rosa_chinensis RchiOBHm_Chr6g0298021
rosa_laevigata RLG00000011542
rosa_multiflora Rmu_co8512357.1_g000001 Rmu_sc0002800.1_g000021
rosa_rugosa Rorug06G0278100
rosa_samantha Rh6AG388400 Rh6BG396600 Rh6CG402600 Rh6DG388800
rosa_wichuraiana Rw6G033900

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 2 cut(s) 360, 1733
Acc36I ACCTGC 2 cut(s) 273, 1889
Acc65I GGTACC 1 cut(s) 3234
AccB1I GGYRCC 3 cut(s) 521, 870, 3234
AccB7I CCANNNNNTGG 1 cut(s) 2546
AccI GTMKAC 2 cut(s) 1394, 1948
AccII CGCG 3 cut(s) 1446, 1932, 1958
AciI CCGC 8 cut(s) 1221, 1833, 1860, 1877, 1910, 1932, 1956, 3242
AclWI GGATC 5 cut(s) 500, 680, 1303, 1541, 3176
AcoI YGGCCR 3 cut(s) 638, 1222, 1604
AcsI RAATTY 4 cut(s) 765, 1135, 1683, 3016
AcuI CTGAAG 2 cut(s) 2837, 2966
AcyI GRCGYC 3 cut(s) 522, 871, 2673
AfaI GTAC 9 cut(s) 350, 566, 1232, 2043, 2327, 2573, 2591, 3050, 3236
AfeI AGCGCT 2 cut(s) 1271, 2798
AflIII ACRYGT 3 cut(s) 1943, 2449, 2628
AjnI CCWGG 9 cut(s) 1277, 2191, 2227, 2310, 2427, 2755, 2913, 3112, 3140
AjuI GAANNNNNNNTTGG 2 cut(s) 1517, 1549
AloI GAACNNNNNNTCC 4 cut(s) 177, 209, 664, 696
Alw21I GWGCWC 2 cut(s) 2913, 2944
Alw26I GTCTC 1 cut(s) 477
AlwI GGATC 5 cut(s) 500, 680, 1303, 1541, 3176
AlwNI CAGNNNCTG 1 cut(s) 898
Ama87I CYCGRG 2 cut(s) 932, 2002
Aor51HI AGCGCT 2 cut(s) 1271, 2798
ApoI RAATTY 4 cut(s) 765, 1135, 1683, 3016
ArsI GACNNNNNNTTYG 4 cut(s) 782, 814, 2312, 2344
Asp700I GAANNNNTTC 1 cut(s) 2954
Asp718I GGTACC 1 cut(s) 3234
AspA2I CCTAGG 1 cut(s) 2707
AspLEI GCGC 7 cut(s) 524, 751, 873, 1272, 1932, 1960, 2799
AsuC2I CCSGG 2 cut(s) 875, 1710
AsuHPI GGTGA 5 cut(s) 1082, 1333, 1874, 2207, 3088
AsuNHI GCTAGC 1 cut(s) 2515
AvaI CYCGRG 2 cut(s) 932, 2002
AvaII GGWCC 7 cut(s) 233, 262, 556, 2320, 2416, 2717, 3110
AvrII CCTAGG 1 cut(s) 2707
BalI TGGCCA 2 cut(s) 640, 1606
BanI GGYRCC 3 cut(s) 521, 870, 3234
BanII GRGCYC 3 cut(s) 663, 2764, 2913
BauI CACGAG 1 cut(s) 97
BbsI GAAGAC 1 cut(s) 2177
Bbv12I GWGCWC 2 cut(s) 2913, 2944
BbvCI CCTCAGC 1 cut(s) 611
BciT130I CCWGG 9 cut(s) 1279, 2193, 2229, 2312, 2429, 2757, 2915, 3114, 3142
BciVI GTATCC 2 cut(s) 1457, 3243
BcnI CCSGG 2 cut(s) 875, 1710
BcoDI GTCTC 1 cut(s) 477
BfmI CTRYAG 4 cut(s) 1407, 1540, 1703, 2397
BfoI RGCGCY 4 cut(s) 525, 874, 1273, 2800
BfuAI ACCTGC 2 cut(s) 273, 1889
BfuI GTATCC 2 cut(s) 1457, 3243
BglII AGATCT 1 cut(s) 2686
BlnI CCTAGG 1 cut(s) 2707
BlpI GCTNAGC 1 cut(s) 1273
Bme18I GGWCC 7 cut(s) 233, 262, 556, 2320, 2416, 2717, 3110
BmeT110I CYCGRG 2 cut(s) 932, 2002
BmrI ACTGGG 2 cut(s) 2556, 2877
BmtI GCTAGC 1 cut(s) 2519
BmuI ACTGGG 2 cut(s) 2556, 2877
BpiI GAAGAC 1 cut(s) 2177
BplI GAGNNNNNCTC 2 cut(s) 2895, 2927
BpmI CTGGAG 4 cut(s) 641, 1795, 2897, 3135
Bpu10I CCTNAGC 2 cut(s) 611, 1437
Bpu1102I GCTNAGC 1 cut(s) 1273
BpuMI CCSGG 2 cut(s) 875, 1710
Bsa29I ATCGAT 1 cut(s) 13
BsaBI GATNNNNATC 3 cut(s) 2346, 2685, 3173
BsaHI GRCGYC 3 cut(s) 522, 871, 2673
BsaI GGTCTC 1 cut(s) 477
BsaJI CCNNGG 8 cut(s) 25, 411, 560, 1709, 2001, 2227, 2428, 2707
Bse118I RCCGGY 1 cut(s) 867
Bse1I ACTGG 3 cut(s) 920, 2551, 2872
Bse3DI GCAATG 3 cut(s) 1736, 2449, 2969
Bse8I GATNNNNATC 3 cut(s) 2346, 2685, 3173
BseBI CCWGG 9 cut(s) 1279, 2193, 2229, 2312, 2429, 2757, 2915, 3114, 3142
BseCI ATCGAT 1 cut(s) 13
BseDI CCNNGG 8 cut(s) 25, 411, 560, 1709, 2001, 2227, 2428, 2707
BseJI GATNNNNATC 3 cut(s) 2346, 2685, 3173
BseMI GCAATG 3 cut(s) 1736, 2449, 2969
BseMII CTCAG 8 cut(s) 602, 800, 890, 1175, 1287, 1883, 2279, 2990
BseNI ACTGG 3 cut(s) 920, 2551, 2872
BseRI GAGGAG 1 cut(s) 465
BseX3I CGGCCG 1 cut(s) 1222
BsgI GTGCAG 2 cut(s) 883, 1917
Bsh1236I CGCG 3 cut(s) 1446, 1932, 1958
Bsh1285I CGRYCG 1 cut(s) 1225
BshNI GGYRCC 3 cut(s) 521, 870, 3234
BshVI ATCGAT 1 cut(s) 13
BsiEI CGRYCG 1 cut(s) 1225
BsiHKAI GWGCWC 2 cut(s) 2913, 2944
BsiHKCI CYCGRG 2 cut(s) 932, 2002
BsiSI CCGG 3 cut(s) 868, 875, 1709
BslFI GGGAC 4 cut(s) 395, 542, 2333, 2844
BsmAI GTCTC 1 cut(s) 477
BsmFI GGGAC 4 cut(s) 395, 542, 2333, 2844
BsmI GAATGC 2 cut(s) 1298, 1785
Bso31I GGTCTC 1 cut(s) 477
BsoBI CYCGRG 2 cut(s) 932, 2002
Bsp1286I GDGCHC 4 cut(s) 663, 2764, 2913, 2944
Bsp1720I GCTNAGC 1 cut(s) 1273
Bsp19I CCATGG 1 cut(s) 25
Bsp68I TCGCGA 1 cut(s) 1446
BspACI CCGC 8 cut(s) 1221, 1833, 1860, 1877, 1910, 1932, 1956, 3242
BspCNI CTCAG 8 cut(s) 603, 801, 891, 1174, 1286, 1884, 2278, 2991
BspDI ATCGAT 1 cut(s) 13
BspFNI CGCG 3 cut(s) 1446, 1932, 1958
BspHI TCATGA 1 cut(s) 478
BspMAI CTGCAG 3 cut(s) 1544, 1707, 2401
BspMI ACCTGC 2 cut(s) 273, 1889
BspOI GCTAGC 1 cut(s) 2519
BspPI GGATC 5 cut(s) 500, 680, 1303, 1541, 3176
BspQI GCTCTTC 2 cut(s) 2363, 2885
BspT107I GGYRCC 3 cut(s) 521, 870, 3234
BspTNI GGTCTC 1 cut(s) 477
BsrDI GCAATG 3 cut(s) 1736, 2449, 2969
BsrFI RCCGGY 1 cut(s) 867
BsrI ACTGG 3 cut(s) 920, 2551, 2872
BssAI RCCGGY 1 cut(s) 867
BssECI CCNNGG 8 cut(s) 25, 411, 560, 1709, 2001, 2227, 2428, 2707
BssNAI GTATAC 1 cut(s) 1949
BssNI GRCGYC 3 cut(s) 522, 871, 2673
BssSI CACGAG 1 cut(s) 97
BssT1I CCWWGG 3 cut(s) 25, 411, 2707
Bst1107I GTATAC 1 cut(s) 1949
Bst2BI CACGAG 1 cut(s) 97
Bst2UI CCWGG 9 cut(s) 1279, 2193, 2229, 2312, 2429, 2757, 2915, 3114, 3142
Bst4CI ACNGT 8 cut(s) 259, 353, 1035, 1408, 2096, 2420, 2931, 2939
Bst6I CTCTTC 3 cut(s) 2363, 2850, 2885
BstACI GRCGYC 3 cut(s) 522, 871, 2673
BstAPI GCANNNNNTGC 2 cut(s) 288, 2222
BstC8I GCNNGC 4 cut(s) 444, 869, 1579, 2517
BstDSI CCRYGG 1 cut(s) 25
BstENI CCTNNNNNAGG 1 cut(s) 892
BstFNI CGCG 3 cut(s) 1446, 1932, 1958
BstH2I RGCGCY 4 cut(s) 525, 874, 1273, 2800
BstHHI GCGC 7 cut(s) 524, 751, 873, 1272, 1932, 1960, 2799
BstMAI GTCTC 1 cut(s) 477
BstMCI CGRYCG 1 cut(s) 1225
BstNI CCWGG 9 cut(s) 1279, 2193, 2229, 2312, 2429, 2757, 2915, 3114, 3142
BstNSI RCATGY 7 cut(s) 716, 1757, 1939, 1947, 2453, 2632, 2731
BstSFI CTRYAG 4 cut(s) 1407, 1540, 1703, 2397
BstUI CGCG 3 cut(s) 1446, 1932, 1958
BstV2I GAAGAC 1 cut(s) 2177
BstX2I RGATCY 1 cut(s) 2686
BstXI CCANNNNNNTGG 1 cut(s) 2413
BstYI RGATCY 1 cut(s) 2686
BstZ17I GTATAC 1 cut(s) 1949
BstZI CGGCCG 1 cut(s) 1222
Bsu15I ATCGAT 1 cut(s) 13
BsuI GTATCC 2 cut(s) 1457, 3243
BsuTUI ATCGAT 1 cut(s) 13
BtgI CCRYGG 1 cut(s) 25
BtgZI GCGATG 1 cut(s) 1523
BtsI GCAGTG 5 cut(s) 303, 510, 1549, 1748, 1848
BtuMI TCGCGA 1 cut(s) 1446
BveI ACCTGC 2 cut(s) 273, 1889
Cac8I GCNNGC 4 cut(s) 444, 869, 1579, 2517
CaiI CAGNNNCTG 1 cut(s) 898
CciI TCATGA 1 cut(s) 478
CfoI GCGC 7 cut(s) 524, 751, 873, 1272, 1932, 1960, 2799
Cfr10I RCCGGY 1 cut(s) 867
ClaI ATCGAT 1 cut(s) 13
CseI GACGC 1 cut(s) 2681
Csp6I GTAC 9 cut(s) 349, 565, 1231, 2042, 2326, 2572, 2590, 3049, 3235
CspCI CAANNNNNGTGG 2 cut(s) 3166, 3201
CviQI GTAC 9 cut(s) 349, 565, 1231, 2042, 2326, 2572, 2590, 3049, 3235
DinI GGCGCC 2 cut(s) 523, 872
DraI TTTAAA 2 cut(s) 1585, 1719
EaeI YGGCCR 3 cut(s) 638, 1222, 1604
EagI CGGCCG 1 cut(s) 1222
Eam1104I CTCTTC 3 cut(s) 2363, 2850, 2885
EarI CTCTTC 3 cut(s) 2363, 2850, 2885
Ecl136II GAGCTC 1 cut(s) 2911
EclXI CGGCCG 1 cut(s) 1222
Eco130I CCWWGG 3 cut(s) 25, 411, 2707
Eco147I AGGCCT 1 cut(s) 1642
Eco24I GRGCYC 3 cut(s) 663, 2764, 2913
Eco31I GGTCTC 1 cut(s) 477
Eco32I GATATC 1 cut(s) 1318
Eco47I GGWCC 7 cut(s) 233, 262, 556, 2320, 2416, 2717, 3110
Eco47III AGCGCT 2 cut(s) 1271, 2798
Eco52I CGGCCG 1 cut(s) 1222
Eco53kI GAGCTC 1 cut(s) 2911
Eco57I CTGAAG 2 cut(s) 2837, 2966
Eco88I CYCGRG 2 cut(s) 932, 2002
EcoICRI GAGCTC 1 cut(s) 2911
EcoNI CCTNNNNNAGG 1 cut(s) 892
EcoO109I RGGNCCY 3 cut(s) 233, 1969, 2320
EcoRI GAATTC 1 cut(s) 1135
EcoRII CCWGG 9 cut(s) 1277, 2191, 2227, 2310, 2427, 2755, 2913, 3112, 3140
EcoRV GATATC 1 cut(s) 1318
EcoT14I CCWWGG 3 cut(s) 25, 411, 2707
EcoT38I GRGCYC 3 cut(s) 663, 2764, 2913
EgeI GGCGCC 2 cut(s) 523, 872
EheI GGCGCC 2 cut(s) 523, 872
ErhI CCWWGG 3 cut(s) 25, 411, 2707
FalI AAGNNNNNCTT 6 cut(s) 647, 679, 1075, 1107, 1219, 1251
FaqI GGGAC 4 cut(s) 395, 542, 2333, 2844
FauNDI CATATG 2 cut(s) 1399, 2734
FblI GTMKAC 2 cut(s) 1394, 1948
FriOI GRGCYC 3 cut(s) 663, 2764, 2913
GlaI GCGC 7 cut(s) 523, 750, 872, 1271, 1931, 1959, 2798
GsuI CTGGAG 4 cut(s) 641, 1795, 2897, 3135
HaeII RGCGCY 4 cut(s) 525, 874, 1273, 2800
HapII CCGG 3 cut(s) 868, 875, 1709
HgaI GACGC 1 cut(s) 2681
HhaI GCGC 7 cut(s) 524, 751, 873, 1272, 1932, 1960, 2799
Hin1I GRCGYC 3 cut(s) 522, 871, 2673
Hin6I GCGC 7 cut(s) 522, 749, 871, 1270, 1930, 1958, 2797
HinP1I GCGC 7 cut(s) 522, 749, 871, 1270, 1930, 1958, 2797
HincII GTYRAC 2 cut(s) 1941, 2634
HindII GTYRAC 2 cut(s) 1941, 2634
HindIII AAGCTT 2 cut(s) 1439, 2295
HpaI GTTAAC 1 cut(s) 1941
HpaII CCGG 3 cut(s) 868, 875, 1709
HphI GGTGA 5 cut(s) 1082, 1333, 1874, 2207, 3088
Hpy166II GTNNAC 9 cut(s) 262, 1341, 1395, 1865, 1941, 1949, 2234, 2474, 2634
Hpy8I GTNNAC 9 cut(s) 262, 1341, 1395, 1865, 1941, 1949, 2234, 2474, 2634
HpyCH4III ACNGT 8 cut(s) 259, 353, 1035, 1408, 2096, 2420, 2931, 2939
HpyCH4IV ACGT 1 cut(s) 347
HpySE526I ACGT 1 cut(s) 347
Hsp92I GRCGYC 3 cut(s) 522, 871, 2673
HspAI GCGC 7 cut(s) 522, 749, 871, 1270, 1930, 1958, 2797
KasI GGCGCC 2 cut(s) 521, 870
KpnI GGTACC 1 cut(s) 3238
KroI GCCGGC 1 cut(s) 867
KroNI GCCGGC 1 cut(s) 869
KspAI GTTAAC 1 cut(s) 1941
LguI GCTCTTC 2 cut(s) 2363, 2885
LmnI GCTCC 6 cut(s) 658, 1267, 2759, 2908, 2916, 2917
MaeII ACGT 1 cut(s) 347
MaeIII GTNAC 7 cut(s) 704, 1087, 1846, 2306, 2332, 2863, 3055
MfeI CAATTG 1 cut(s) 1259
MflI RGATCY 1 cut(s) 2686
MhlI GDGCHC 4 cut(s) 663, 2764, 2913, 2944
MlsI TGGCCA 2 cut(s) 640, 1606
MluNI TGGCCA 2 cut(s) 640, 1606
Mly113I GGCGCC 2 cut(s) 522, 871
MlyI GAGTC 4 cut(s) 71, 955, 1670, 3197
MmeI TCCRAC 4 cut(s) 470, 939, 1824, 2744
Mox20I TGGCCA 2 cut(s) 640, 1606
MroNI GCCGGC 1 cut(s) 867
MroXI GAANNNNTTC 1 cut(s) 2954
MscI TGGCCA 2 cut(s) 640, 1606
MslI CAYNNNNRTG 4 cut(s) 711, 1823, 2193, 2244
Msp20I TGGCCA 2 cut(s) 640, 1606
MspA1I CMGCKG 3 cut(s) 610, 2815, 2923
MspI CCGG 3 cut(s) 868, 875, 1709
MunI CAATTG 1 cut(s) 1259
Mva1269I GAATGC 2 cut(s) 1298, 1785
MvaI CCWGG 9 cut(s) 1279, 2193, 2229, 2312, 2429, 2757, 2915, 3114, 3142
MvnI CGCG 3 cut(s) 1446, 1932, 1958
NaeI GCCGGC 1 cut(s) 869
NarI GGCGCC 2 cut(s) 522, 871
NciI CCSGG 2 cut(s) 875, 1710
NcoI CCATGG 1 cut(s) 25
NdeI CATATG 2 cut(s) 1399, 2734
NgoMIV GCCGGC 1 cut(s) 867
NheI GCTAGC 1 cut(s) 2515
NmeAIII GCCGAG 1 cut(s) 601
NmuCI GTSAC 5 cut(s) 704, 1846, 2306, 2332, 2863
NruI TCGCGA 1 cut(s) 1446
NspI RCATGY 7 cut(s) 716, 1757, 1939, 1947, 2453, 2632, 2731
PagI TCATGA 1 cut(s) 478
PceI AGGCCT 1 cut(s) 1642
PciI ACATGT 3 cut(s) 1943, 2449, 2628
PciSI GCTCTTC 2 cut(s) 2363, 2885
PctI GAATGC 2 cut(s) 1298, 1785
PdiI GCCGGC 1 cut(s) 869
PdmI GAANNNNTTC 1 cut(s) 2954
PfeI GAWTC 9 cut(s) 5, 15, 116, 430, 649, 1623, 2176, 2206, 3083
PflMI CCANNNNNTGG 1 cut(s) 2546
PfoI TCCNGGA 3 cut(s) 2755, 2913, 3112
PleI GAGTC 4 cut(s) 70, 954, 1669, 3197
PluTI GGCGCC 2 cut(s) 525, 874
PpsI GAGTC 4 cut(s) 70, 954, 1669, 3197
PpuMI RGGWCCY 2 cut(s) 233, 2320
PscI ACATGT 3 cut(s) 1943, 2449, 2628
PsiI TTATAA 2 cut(s) 360, 1733
Psp124BI GAGCTC 1 cut(s) 2913
Psp5II RGGWCCY 2 cut(s) 233, 2320
Psp6I CCWGG 9 cut(s) 1277, 2191, 2227, 2310, 2427, 2755, 2913, 3112, 3140
PspGI CCWGG 9 cut(s) 1277, 2191, 2227, 2310, 2427, 2755, 2913, 3112, 3140
PspPPI RGGWCCY 2 cut(s) 233, 2320
PstI CTGCAG 3 cut(s) 1544, 1707, 2401
PstNI CAGNNNCTG 1 cut(s) 898
PsuI RGATCY 1 cut(s) 2686
PvuII CAGCTG 3 cut(s) 610, 2815, 2923
RruI TCGCGA 1 cut(s) 1446
RsaI GTAC 9 cut(s) 350, 566, 1232, 2043, 2327, 2573, 2591, 3050, 3236
RsaNI GTAC 9 cut(s) 349, 565, 1231, 2042, 2326, 2572, 2590, 3049, 3235
RseI CAYNNNNRTG 4 cut(s) 711, 1823, 2193, 2244
SacI GAGCTC 1 cut(s) 2913
SapI GCTCTTC 2 cut(s) 2363, 2885
SchI GAGTC 4 cut(s) 71, 955, 1670, 3197
SduI GDGCHC 4 cut(s) 663, 2764, 2913, 2944
SfcI CTRYAG 4 cut(s) 1407, 1540, 1703, 2397
SfoI GGCGCC 2 cut(s) 523, 872
SinI GGWCC 7 cut(s) 233, 262, 556, 2320, 2416, 2717, 3110
SmiMI CAYNNNNRTG 4 cut(s) 711, 1823, 2193, 2244
SseBI AGGCCT 1 cut(s) 1642
SsiI CCGC 8 cut(s) 1221, 1833, 1860, 1877, 1910, 1932, 1956, 3242
SspDI GGCGCC 2 cut(s) 521, 870
SspI AATATT 1 cut(s) 1364
SstI GAGCTC 1 cut(s) 2913
StuI AGGCCT 1 cut(s) 1642
StyI CCWWGG 3 cut(s) 25, 411, 2707
TaaI ACNGT 8 cut(s) 259, 353, 1035, 1408, 2096, 2420, 2931, 2939
TaiI ACGT 1 cut(s) 350
TaqI TCGA 8 cut(s) 13, 65, 177, 726, 801, 942, 2288, 3201
TaqII GACCGA 1 cut(s) 544
TatI WGTACW 1 cut(s) 2325
TauI GCSGC 5 cut(s) 1224, 1835, 1880, 1935, 1958
TfiI GAWTC 9 cut(s) 5, 15, 116, 430, 649, 1623, 2176, 2206, 3083
TseFI GTSAC 5 cut(s) 704, 1846, 2306, 2332, 2863
Tsp45I GTSAC 5 cut(s) 704, 1846, 2306, 2332, 2863
TspDTI ATGAA 8 cut(s) 1116, 1148, 1664, 2177, 2358, 2447, 2590, 2697
Van91I CCANNNNNTGG 1 cut(s) 2546
VpaK11BI GGWCC 7 cut(s) 233, 262, 556, 2320, 2416, 2717, 3110
XagI CCTNNNNNAGG 1 cut(s) 892
XapI RAATTY 4 cut(s) 765, 1135, 1683, 3016
XbaI TCTAGA 2 cut(s) 2026, 3206
XceI RCATGY 7 cut(s) 716, 1757, 1939, 1947, 2453, 2632, 2731
XmaJI CCTAGG 1 cut(s) 2707
XmiI GTMKAC 2 cut(s) 1394, 1948
XmnI GAANNNNTTC 1 cut(s) 2954
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.