Rh6BG396600
ERF Family

Probably involved in the RNA silencing pathway and required for the generation of small interfering RNAs (siRNAs)

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr6B
OG0017992
Physical Location & Seq
Reverse (-)
62448910 .. 62452693
3784 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh6BG396600.1

Sequence Viewer

Length: 3318 bp
ATGGAATCACATCGATTCTTGAACCATGGCTCTCCACCAATACTAGGGTCGTGTTCAATGGAGTCGATCAATCCACACTACACATACCTAAACAACACGAGTTGTGCTATGGAGGATTCTTATTCTCTAGGGCGTTGTGAAACTGAAACCCTATTCAGGCTTTACGATGTGCGTGTCGAGATCGGAACCCTAGTTCGCCAAGACCAAGATGAGTTCTTGGTTGCTTGGGAAGGACCCCCTAAAGGAGTTTCCTTTACTGTGGACCCTTCTACTAGGACTTGCAGGTTTTGCTTGACACAAAACACTGCCTTTTCGTATGGTGGTAGGAGAAACAAGCGTGTGAGGACGTACTGTGATTATAAAGTGGAGTTTTTGGTTAGGGACATCAGTGAGTTCAAGCATTACGAAACCTTGGAGATGGATTTTGTGATTCTGTTGAGGCTGGCTTGGTCGCCATGTGTTTCGTGCAGGTCTCCTCATGATGAGAGTTGGATCAGGACTACTGATTTCACTGCAAATGGCGCCATTGGAAGGAGTAATTTCTACAAGTTTTCGGTCCCTCGGTACTATGGGGCCGAGTGGAACAAGGCCATTGATTATCTTAGACAGCTGAGGGTGCTGGAGTTAGATATAAAATGGCCACTTGGGATTCAGAAGGAGCCCTTTTTCGGGATGCCTAATTCCGATCCTTTCTACTCTATTGGTCACAAACATGCAATTTGCTTCGAGATTATCTTCTTGGTTAATGCGGTAATGCACAAGGGAATTCTCAATCAGCATCAGTTGTCAGACAGCTTCTTCAATTTACTGAGAAGCCAATCAAAGGATGTCAATGTGGCTGCATTGAACTACTTATATTCGTGCAGCCGGCGCCCGGTGTTAGATGCCTGTCAGAGGCTGAGAGCTGTGCAAGACTGGTTTGTGGCAAATCCCGAGCTTTTCGAGAGTCCTAAAAGGTTGGATGATATTGCTCATGTCAGGAGGTTGGTCATTACCCCAACTAAAGCCTATTGTATTCCACCAGAGGCTCAACTGTCAAATAGGGTTCTTAGAAAATACAAACAAATAGGTGATCGGTTTCTAAGGGTTACTTTTATGGATGAAGGAATGCAGACTATTTATTCCAATGCTATGAATTCTTATGGGGAAAAATCCTGCCCTCAGAGAACAAAAGTATTTGGAAGGGTTGAGGCCATTCTGAAAAATGGATTTGACTTATGCGGCCGAAAGTACTCTTTCTTGGCCTTCTCATCCAATCAATTGAGGGAGCGCTCGGCCTGGTTTTTTGCTGAATGCAAGAGCATTACGATCCACGATATCCAAACTTGGATGGGGAAGTTCACCAACAGAAATGTTGCAAAATATTCTGCTAGAATGGGTCAATGCTTTTCGTCTACATATGCAACGGTAGAAGTTCCATCAAGCAAGGTTAATCCTAAGCTTCGCGATATTGAGAGGAATGGATACAATTTTTCAGATGGGATTGGTAAGATTTCCCCTGCTCTTGTGATGGAAGTTGCAAAGAAACTGAAATTGGACTGCAGTGATCCTCCTTGTGCATACCAAATTAGATATGCTGGCTTTAAAGGGGTTATTGCTTGTTGGCCAAGCGAAGGGGATAGATTCCAGCTCTCTTTAAGGCCTAGCATGAACAAGTTCGAGTCGCACCATACTACATTGGAAATTTGTTCTTGGACTAGGCTGCAGCCGGGGTTTTTAAACAGGCAGATTATAACATTGCTTTCAACACTGCATGTTCCAGATATGGTATTCTGGAGAATGCAGCAGCAAATGGTTTCCAACTTAAAACTGATGCTCACAGACATGGATGCCGCATTTGATGTTGTCACTGCCTCATGCGGTGAACAAGGAAATGCGGCAGCTATAATGCTGAGTGCAGGTTTTAATCCACAGACTGAACCTCATTTGCGCGGCATGTTAACATGTATACAAGCCGCGCAGCTTTGGGGCCTCCGAGAAAAGTCTAGGATTTTTGTTCCCTCGGGGAGATGGCTTATGGGATGTCTAGATGAGCTAGGGGTACTTGAGCAAGGTCAGTGCTTTATCCAAGTGTCTAACCCATCACTTGAAAACTGTTTTGCAAAGCATGGCTCTAGGTTTTGTCCGATAGAGAAAAACCTACAAGTAATAAAAGGATTAGTTGTGGTAGCAAAGAATCCATGTCTTCATCCAGGTGATATAAGGATTCTTGAAGCAGTTGATGCCCCAGGTTTACACCACTTACATGATTGCCTTGTTTTCCCTCAGAAGGGTTATAGGCCACACTCGAACGAAGCTTCTGGAAGTGACCTGGATGGGGACCTGTACTTTGTCACATGGGATGAAAATCTTATTCCCCCTAGTAAGAAGAGCGAGAAACCTATGGAGTATGATGCTGCAGAAGCCAAAACTTTGGACCGTCCTGTCACTAACCAGGATATTATAGAGTTTTTTGTGAGAAACATGGCGAATGAGAACCTGGGGGCGATATGCAATGCACATGTAGTTCATGCTGACATCAGTGAACATGGGGCTTCGGATGACAACTGCATCAAACTAGCGAAGCTAGCAGCAATAGCAGTTGATCTACCAAAAACTGGGAAGATTGTTCCAATGCCATCGTACCTAAAACCAGAGTTGTACCCAGATTTCATGGGGAAAGAGGGCTTCAAGTCTTACATGTCAAGTAAAATCTTGGGGGTACTCTATCGCCAGATCACAGATGCCTATGATGAAGATCTGGCAACCTCTTTTAACCTACGTTATGGTCCAACTGACATCCCATATGATATTGATTTGGAGGTTCCAGGAGCGCACAATATCATAGTAGAAGCATTGGAGAGAAAGCGCTCTTATGATTTTCAGCTGAAGGGTCTAATGGGACAATATAATGTTGTGAGGGAAGAAGAGATTGTCACTGGGTATGTTTGGTCAATGCCGAAGAGCAGGAAGCGAGGGGAGCTCCAGGAGCAGCTGAAACAGTCTTACAGTGCTCTGAAGAAAGAGTTCAGGCAATGTTTTGAGAGAATTGATGGAGATTTCAAGTCGCTGAGTAATGATCAGAAGAATTTTTTGTATGAGCAGAAGGCATCAGCTTGGTACATGGTAACTTATCATCCTAAGTTGGTGAAAGAATCACTCTATTTGCAAGGGCCAGATGGTCCTGGAGATGTGCCGATGTTGAGCTTTGCCTGGATTGCAACTGACTACCTTGCTCGGATCAAGATCAAACGTTGTGGAGTGGGAATGATCGACTCTAGAAAGCCAATCAACTCTTTGAGAAGGTACCTTGCGGATAGGATACGGACCGCCATTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0000003 GO:0001172 GO:0002252 GO:0002376 GO:0003006 GO:0003674 GO:0003824 GO:0003968 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0006139 GO:0006396 GO:0006725 GO:0006807 GO:0006950 GO:0006952 GO:0006955 GO:0007154 GO:0007275 GO:0008037 GO:0008150 GO:0008152 GO:0009058 GO:0009059 GO:0009605 GO:0009607 GO:0009615 GO:0009616 GO:0009791 GO:0009856 GO:0009875 GO:0009888 GO:0009892 GO:0009908 GO:0009987 GO:0010033 GO:0010073 GO:0010074 GO:0010267 GO:0010467 GO:0010468 GO:0010492 GO:0010605 GO:0010608 GO:0010629 GO:0014070 GO:0016070 GO:0016246 GO:0016441 GO:0016458 GO:0016740 GO:0016772 GO:0016779 GO:0018130 GO:0019222 GO:0019438 GO:0019827 GO:0022414 GO:0030422 GO:0031047 GO:0031050 GO:0032501 GO:0032502 GO:0032774 GO:0034062 GO:0034641 GO:0034654 GO:0035194 GO:0035821 GO:0040029 GO:0042221 GO:0043170 GO:0043207 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043331 GO:0044003 GO:0044237 GO:0044238 GO:0044249 GO:0044271 GO:0044403 GO:0044419 GO:0044424 GO:0044464 GO:0044706 GO:0045087 GO:0046483 GO:0048366 GO:0048367 GO:0048437 GO:0048438 GO:0048440 GO:0048467 GO:0048507 GO:0048519 GO:0048544 GO:0048608 GO:0048731 GO:0048827 GO:0048856 GO:0050789 GO:0050896 GO:0051607 GO:0051701 GO:0051704 GO:0051707 GO:0051716 GO:0051817 GO:0052018 GO:0052249 GO:0060255 GO:0061458 GO:0065007 GO:0070887 GO:0070918 GO:0071310 GO:0071359 GO:0071407 GO:0071704 GO:0090304 GO:0090567 GO:0097659 GO:0097747 GO:0098542 GO:0098586 GO:0098727 GO:0099402 GO:0140098 GO:1901360 GO:1901362 GO:1901576 GO:1901698 GO:1901699
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
RdRP

Protein Analysis

1105

Amino Acids

126.15

Weight (kDa)

8.4

Isoelectric Point (pI)

42.37

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
RDR6_2nd PF24577 53 - 206 8.7e-50 RNA-dependent RNA polymerase 6 second domain
RdRP_helical PF26252 232 - 313 1.3e-15 RNA dependent RNA polymerase, helical domain
RdRP PF05183 331 - 906 1.4e-189 RNA dependent RNA polymerase
RdRP_head PF26253 928 - 1078 3e-37 RdRP, head domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0017992)

Species Orthologous Gene IDs
fragaria_vesca FvH4_2g29160
rosa_chinensis RchiOBHm_Chr6g0298021
rosa_laevigata RLG00000011542
rosa_multiflora Rmu_co8512357.1_g000001 Rmu_sc0002800.1_g000021
rosa_rugosa Rorug06G0278100
rosa_samantha Rh6AG388400 Rh6BG396600 Rh6CG402600 Rh6DG388800
rosa_wichuraiana Rw6G033900

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 2 cut(s) 360, 1733
AasI GACNNNNNNGTC 1 cut(s) 2423
Acc36I ACCTGC 3 cut(s) 273, 459, 1889
Acc65I GGTACC 1 cut(s) 3285
AccB1I GGYRCC 3 cut(s) 521, 870, 3285
AccB7I CCANNNNNTGG 1 cut(s) 2597
AccI GTMKAC 2 cut(s) 1394, 1948
AccII CGCG 3 cut(s) 1446, 1932, 1958
AciI CCGC 9 cut(s) 749, 1221, 1833, 1860, 1877, 1932, 1956, 3293, 3309
AclI AACGTT 1 cut(s) 3232
AclWI GGATC 5 cut(s) 500, 680, 1303, 1541, 3227
AcoI YGGCCR 3 cut(s) 638, 1222, 1604
AcsI RAATTY 4 cut(s) 765, 1135, 1683, 3067
AcuI CTGAAG 2 cut(s) 2888, 3017
AcyI GRCGYC 2 cut(s) 522, 871
AfeI AGCGCT 2 cut(s) 1271, 2849
AflIII ACRYGT 3 cut(s) 1943, 2500, 2679
AjuI GAANNNNNNNTTGG 2 cut(s) 1517, 1549
AloI GAACNNNNNNTCC 2 cut(s) 177, 209
Alw21I GWGCWC 2 cut(s) 2964, 2995
Alw26I GTCTC 1 cut(s) 477
AlwI GGATC 5 cut(s) 500, 680, 1303, 1541, 3227
AlwNI CAGNNNCTG 1 cut(s) 898
Ama87I CYCGRG 2 cut(s) 932, 2002
Aor51HI AGCGCT 2 cut(s) 1271, 2849
ApoI RAATTY 4 cut(s) 765, 1135, 1683, 3067
ArsI GACNNNNNNTTYG 2 cut(s) 2312, 2344
Asp700I GAANNNNTTC 2 cut(s) 1655, 3005
Asp718I GGTACC 1 cut(s) 3285
AspLEI GCGC 7 cut(s) 524, 873, 1272, 1932, 1960, 2815, 2850
AsuC2I CCSGG 2 cut(s) 875, 1710
AsuHPI GGTGA 5 cut(s) 1082, 1333, 1874, 2207, 3139
AsuNHI GCTAGC 1 cut(s) 2566
AvaI CYCGRG 2 cut(s) 932, 2002
AvaII GGWCC 8 cut(s) 233, 262, 556, 2320, 2416, 2768, 3161, 3306
BalI TGGCCA 2 cut(s) 640, 1606
BanI GGYRCC 3 cut(s) 521, 870, 3285
BanII GRGCYC 2 cut(s) 663, 2964
BauI CACGAG 1 cut(s) 97
BbsI GAAGAC 1 cut(s) 2177
Bbv12I GWGCWC 2 cut(s) 2964, 2995
BbvCI CCTCAGC 1 cut(s) 611
BciVI GTATCC 2 cut(s) 1457, 3294
BclI TGATCA 1 cut(s) 3058
BcnI CCSGG 2 cut(s) 875, 1710
BcoDI GTCTC 1 cut(s) 477
BfmI CTRYAG 3 cut(s) 1540, 1703, 2397
BfoI RGCGCY 4 cut(s) 525, 874, 1273, 2851
BfuAI ACCTGC 3 cut(s) 273, 459, 1889
BfuI GTATCC 2 cut(s) 1457, 3294
BglII AGATCT 1 cut(s) 2737
BmcAI AGTACT 1 cut(s) 1232
Bme18I GGWCC 8 cut(s) 233, 262, 556, 2320, 2416, 2768, 3161, 3306
BmeT110I CYCGRG 2 cut(s) 932, 2002
BmrI ACTGGG 2 cut(s) 2607, 2928
BmtI GCTAGC 1 cut(s) 2570
BmuI ACTGGG 2 cut(s) 2607, 2928
BpiI GAAGAC 1 cut(s) 2177
BplI GAGNNNNNCTC 2 cut(s) 2946, 2978
BpmI CTGGAG 4 cut(s) 641, 1795, 2948, 3186
Bpu10I CCTNAGC 2 cut(s) 611, 1437
BpuEI CTTGAG 1 cut(s) 2066
BpuMI CCSGG 2 cut(s) 875, 1710
Bsa29I ATCGAT 1 cut(s) 13
BsaBI GATNNNNATC 3 cut(s) 2346, 2736, 3224
BsaHI GRCGYC 2 cut(s) 522, 871
BsaI GGTCTC 1 cut(s) 477
BsaJI CCNNGG 7 cut(s) 25, 411, 560, 1709, 2001, 2227, 2479
Bse118I RCCGGY 1 cut(s) 867
Bse1I ACTGG 3 cut(s) 920, 2602, 2923
Bse3DI GCAATG 3 cut(s) 1736, 2500, 3020
Bse8I GATNNNNATC 3 cut(s) 2346, 2736, 3224
BseCI ATCGAT 1 cut(s) 13
BseDI CCNNGG 7 cut(s) 25, 411, 560, 1709, 2001, 2227, 2479
BseJI GATNNNNATC 3 cut(s) 2346, 2736, 3224
BseMI GCAATG 3 cut(s) 1736, 2500, 3020
BseMII CTCAG 7 cut(s) 602, 800, 890, 1175, 1883, 2279, 3041
BseNI ACTGG 3 cut(s) 920, 2602, 2923
BseRI GAGGAG 1 cut(s) 465
BseX3I CGGCCG 1 cut(s) 1222
BsgI GTGCAG 3 cut(s) 487, 883, 1917
Bsh1236I CGCG 3 cut(s) 1446, 1932, 1958
Bsh1285I CGRYCG 1 cut(s) 1225
BshNI GGYRCC 3 cut(s) 521, 870, 3285
BshVI ATCGAT 1 cut(s) 13
BsiEI CGRYCG 1 cut(s) 1225
BsiHKAI GWGCWC 2 cut(s) 2964, 2995
BsiHKCI CYCGRG 2 cut(s) 932, 2002
BsiSI CCGG 3 cut(s) 868, 875, 1709
BslFI GGGAC 4 cut(s) 395, 542, 2333, 2895
BsmAI GTCTC 1 cut(s) 477
BsmFI GGGAC 4 cut(s) 395, 542, 2333, 2895
BsmI GAATGC 3 cut(s) 1113, 1298, 1785
Bso31I GGTCTC 1 cut(s) 477
BsoBI CYCGRG 2 cut(s) 932, 2002
Bsp1286I GDGCHC 3 cut(s) 663, 2964, 2995
Bsp19I CCATGG 1 cut(s) 25
Bsp68I TCGCGA 1 cut(s) 1446
BspACI CCGC 9 cut(s) 749, 1221, 1833, 1860, 1877, 1932, 1956, 3293, 3309
BspCNI CTCAG 7 cut(s) 603, 801, 891, 1174, 1884, 2278, 3042
BspDI ATCGAT 1 cut(s) 13
BspFNI CGCG 3 cut(s) 1446, 1932, 1958
BspHI TCATGA 1 cut(s) 478
BspMAI CTGCAG 3 cut(s) 1544, 1707, 2401
BspMI ACCTGC 3 cut(s) 273, 459, 1889
BspOI GCTAGC 1 cut(s) 2570
BspPI GGATC 5 cut(s) 500, 680, 1303, 1541, 3227
BspQI GCTCTTC 2 cut(s) 2363, 2936
BspT107I GGYRCC 3 cut(s) 521, 870, 3285
BspTNI GGTCTC 1 cut(s) 477
BsrDI GCAATG 3 cut(s) 1736, 2500, 3020
BsrFI RCCGGY 1 cut(s) 867
BsrI ACTGG 3 cut(s) 920, 2602, 2923
BssAI RCCGGY 1 cut(s) 867
BssECI CCNNGG 7 cut(s) 25, 411, 560, 1709, 2001, 2227, 2479
BssNAI GTATAC 1 cut(s) 1949
BssNI GRCGYC 2 cut(s) 522, 871
BssSI CACGAG 1 cut(s) 97
BssT1I CCWWGG 2 cut(s) 25, 411
Bst1107I GTATAC 1 cut(s) 1949
Bst2BI CACGAG 1 cut(s) 97
Bst4CI ACNGT 8 cut(s) 259, 353, 1035, 1408, 2096, 2420, 2982, 2990
Bst6I CTCTTC 3 cut(s) 2363, 2901, 2936
BstACI GRCGYC 2 cut(s) 522, 871
BstAPI GCANNNNNTGC 2 cut(s) 288, 2222
BstC8I GCNNGC 4 cut(s) 444, 869, 1579, 2568
BstDSI CCRYGG 1 cut(s) 25
BstENI CCTNNNNNAGG 1 cut(s) 892
BstFNI CGCG 3 cut(s) 1446, 1932, 1958
BstH2I RGCGCY 4 cut(s) 525, 874, 1273, 2851
BstHHI GCGC 7 cut(s) 524, 873, 1272, 1932, 1960, 2815, 2850
BstMAI GTCTC 1 cut(s) 477
BstMCI CGRYCG 1 cut(s) 1225
BstNSI RCATGY 6 cut(s) 716, 1757, 1939, 1947, 2504, 2683
BstSFI CTRYAG 3 cut(s) 1540, 1703, 2397
BstUI CGCG 3 cut(s) 1446, 1932, 1958
BstV2I GAAGAC 1 cut(s) 2177
BstX2I RGATCY 1 cut(s) 2737
BstXI CCANNNNNNTGG 1 cut(s) 2413
BstYI RGATCY 1 cut(s) 2737
BstZ17I GTATAC 1 cut(s) 1949
BstZI CGGCCG 1 cut(s) 1222
Bsu15I ATCGAT 1 cut(s) 13
BsuI GTATCC 2 cut(s) 1457, 3294
BsuTUI ATCGAT 1 cut(s) 13
BtgI CCRYGG 1 cut(s) 25
BtsI GCAGTG 5 cut(s) 303, 510, 1549, 1748, 1848
BtuMI TCGCGA 1 cut(s) 1446
BveI ACCTGC 3 cut(s) 273, 459, 1889
Cac8I GCNNGC 4 cut(s) 444, 869, 1579, 2568
CaiI CAGNNNCTG 1 cut(s) 898
CciI TCATGA 1 cut(s) 478
CfoI GCGC 7 cut(s) 524, 873, 1272, 1932, 1960, 2815, 2850
Cfr10I RCCGGY 1 cut(s) 867
ClaI ATCGAT 1 cut(s) 13
CpoI CGGWCCG 1 cut(s) 3306
CspCI CAANNNNNGTGG 2 cut(s) 3217, 3252
CspI CGGWCCG 1 cut(s) 3306
DinI GGCGCC 2 cut(s) 523, 872
DraI TTTAAA 2 cut(s) 1585, 1719
DrdI GACNNNNNNGTC 1 cut(s) 2423
DseDI GACNNNNNNGTC 1 cut(s) 2423
EaeI YGGCCR 3 cut(s) 638, 1222, 1604
EagI CGGCCG 1 cut(s) 1222
Eam1104I CTCTTC 3 cut(s) 2363, 2901, 2936
EarI CTCTTC 3 cut(s) 2363, 2901, 2936
Ecl136II GAGCTC 1 cut(s) 2962
EclXI CGGCCG 1 cut(s) 1222
Eco130I CCWWGG 2 cut(s) 25, 411
Eco147I AGGCCT 1 cut(s) 1642
Eco24I GRGCYC 2 cut(s) 663, 2964
Eco31I GGTCTC 1 cut(s) 477
Eco32I GATATC 1 cut(s) 1318
Eco47I GGWCC 8 cut(s) 233, 262, 556, 2320, 2416, 2768, 3161, 3306
Eco47III AGCGCT 2 cut(s) 1271, 2849
Eco52I CGGCCG 1 cut(s) 1222
Eco53kI GAGCTC 1 cut(s) 2962
Eco57I CTGAAG 2 cut(s) 2888, 3017
Eco88I CYCGRG 2 cut(s) 932, 2002
EcoICRI GAGCTC 1 cut(s) 2962
EcoNI CCTNNNNNAGG 1 cut(s) 892
EcoO109I RGGNCCY 3 cut(s) 233, 1969, 2320
EcoRI GAATTC 2 cut(s) 765, 1135
EcoRV GATATC 1 cut(s) 1318
EcoT14I CCWWGG 2 cut(s) 25, 411
EcoT38I GRGCYC 2 cut(s) 663, 2964
EgeI GGCGCC 2 cut(s) 523, 872
EheI GGCGCC 2 cut(s) 523, 872
ErhI CCWWGG 2 cut(s) 25, 411
FalI AAGNNNNNCTT 4 cut(s) 647, 679, 1075, 1107
FaqI GGGAC 4 cut(s) 395, 542, 2333, 2895
FauNDI CATATG 2 cut(s) 1399, 2785
FbaI TGATCA 1 cut(s) 3058
FblI GTMKAC 2 cut(s) 1394, 1948
FriOI GRGCYC 2 cut(s) 663, 2964
GlaI GCGC 7 cut(s) 523, 872, 1271, 1931, 1959, 2814, 2849
GsuI CTGGAG 4 cut(s) 641, 1795, 2948, 3186
HaeII RGCGCY 4 cut(s) 525, 874, 1273, 2851
HapII CCGG 3 cut(s) 868, 875, 1709
HhaI GCGC 7 cut(s) 524, 873, 1272, 1932, 1960, 2815, 2850
Hin1I GRCGYC 2 cut(s) 522, 871
Hin6I GCGC 7 cut(s) 522, 871, 1270, 1930, 1958, 2813, 2848
HinP1I GCGC 7 cut(s) 522, 871, 1270, 1930, 1958, 2813, 2848
HincII GTYRAC 1 cut(s) 1941
HindII GTYRAC 1 cut(s) 1941
HindIII AAGCTT 2 cut(s) 1439, 2295
HpaI GTTAAC 1 cut(s) 1941
HpaII CCGG 3 cut(s) 868, 875, 1709
HphI GGTGA 5 cut(s) 1082, 1333, 1874, 2207, 3139
Hpy166II GTNNAC 8 cut(s) 262, 1341, 1395, 1865, 1941, 1949, 2234, 2525
Hpy8I GTNNAC 8 cut(s) 262, 1341, 1395, 1865, 1941, 1949, 2234, 2525
HpyCH4III ACNGT 8 cut(s) 259, 353, 1035, 1408, 2096, 2420, 2982, 2990
HpyCH4IV ACGT 3 cut(s) 347, 2761, 3232
HpySE526I ACGT 3 cut(s) 347, 2761, 3232
Hsp92I GRCGYC 2 cut(s) 522, 871
HspAI GCGC 7 cut(s) 522, 871, 1270, 1930, 1958, 2813, 2848
KasI GGCGCC 2 cut(s) 521, 870
KpnI GGTACC 1 cut(s) 3289
KroI GCCGGC 1 cut(s) 867
KroNI GCCGGC 1 cut(s) 869
Ksp22I TGATCA 1 cut(s) 3058
KspAI GTTAAC 1 cut(s) 1941
LguI GCTCTTC 2 cut(s) 2363, 2936
LmnI GCTCC 6 cut(s) 658, 1267, 2810, 2959, 2967, 2968
MaeII ACGT 3 cut(s) 347, 2761, 3232
MaeIII GTNAC 8 cut(s) 704, 1087, 1846, 2306, 2332, 2425, 2914, 3106
MfeI CAATTG 1 cut(s) 1259
MflI RGATCY 1 cut(s) 2737
MhlI GDGCHC 3 cut(s) 663, 2964, 2995
MlsI TGGCCA 2 cut(s) 640, 1606
MluNI TGGCCA 2 cut(s) 640, 1606
Mly113I GGCGCC 2 cut(s) 522, 871
MlyI GAGTC 4 cut(s) 71, 955, 1670, 3248
MmeI TCCRAC 4 cut(s) 470, 939, 1824, 2795
Mox20I TGGCCA 2 cut(s) 640, 1606
MroNI GCCGGC 1 cut(s) 867
MroXI GAANNNNTTC 2 cut(s) 1655, 3005
MscI TGGCCA 2 cut(s) 640, 1606
MseI TTAA 9 cut(s) 744, 1431, 1584, 1637, 1718, 1805, 1905, 1940, 2754
MslI CAYNNNNRTG 4 cut(s) 711, 1823, 2193, 2244
Msp20I TGGCCA 2 cut(s) 640, 1606
MspA1I CMGCKG 3 cut(s) 610, 2866, 2974
MspI CCGG 3 cut(s) 868, 875, 1709
MunI CAATTG 1 cut(s) 1259
Mva1269I GAATGC 3 cut(s) 1113, 1298, 1785
MvnI CGCG 3 cut(s) 1446, 1932, 1958
NaeI GCCGGC 1 cut(s) 869
NarI GGCGCC 2 cut(s) 522, 871
NciI CCSGG 2 cut(s) 875, 1710
NcoI CCATGG 1 cut(s) 25
NdeI CATATG 2 cut(s) 1399, 2785
NgoMIV GCCGGC 1 cut(s) 867
NheI GCTAGC 1 cut(s) 2566
NmeAIII GCCGAG 2 cut(s) 601, 1253
NmuCI GTSAC 6 cut(s) 704, 1846, 2306, 2332, 2425, 2914
NruI TCGCGA 1 cut(s) 1446
NspI RCATGY 6 cut(s) 716, 1757, 1939, 1947, 2504, 2683
PagI TCATGA 1 cut(s) 478
PceI AGGCCT 1 cut(s) 1642
PciI ACATGT 3 cut(s) 1943, 2500, 2679
PciSI GCTCTTC 2 cut(s) 2363, 2936
PctI GAATGC 3 cut(s) 1113, 1298, 1785
PdiI GCCGGC 1 cut(s) 869
PdmI GAANNNNTTC 2 cut(s) 1655, 3005
PfeI GAWTC 9 cut(s) 5, 15, 116, 430, 649, 1623, 2176, 2206, 3134
PflMI CCANNNNNTGG 1 cut(s) 2597
PfoI TCCNGGA 3 cut(s) 2806, 2964, 3163
PleI GAGTC 4 cut(s) 70, 954, 1669, 3248
PluTI GGCGCC 2 cut(s) 525, 874
PpsI GAGTC 4 cut(s) 70, 954, 1669, 3248
PpuMI RGGWCCY 2 cut(s) 233, 2320
PscI ACATGT 3 cut(s) 1943, 2500, 2679
PsiI TTATAA 2 cut(s) 360, 1733
Psp124BI GAGCTC 1 cut(s) 2964
Psp1406I AACGTT 1 cut(s) 3232
Psp5II RGGWCCY 2 cut(s) 233, 2320
PspPPI RGGWCCY 2 cut(s) 233, 2320
PstI CTGCAG 3 cut(s) 1544, 1707, 2401
PstNI CAGNNNCTG 1 cut(s) 898
PsuI RGATCY 1 cut(s) 2737
PvuII CAGCTG 3 cut(s) 610, 2866, 2974
RruI TCGCGA 1 cut(s) 1446
RseI CAYNNNNRTG 4 cut(s) 711, 1823, 2193, 2244
Rsr2I CGGWCCG 1 cut(s) 3306
RsrII CGGWCCG 1 cut(s) 3306
SacI GAGCTC 1 cut(s) 2964
SapI GCTCTTC 2 cut(s) 2363, 2936
SaqAI TTAA 9 cut(s) 744, 1431, 1584, 1637, 1718, 1805, 1905, 1940, 2754
ScaI AGTACT 1 cut(s) 1232
SchI GAGTC 4 cut(s) 71, 955, 1670, 3248
SduI GDGCHC 3 cut(s) 663, 2964, 2995
SfcI CTRYAG 3 cut(s) 1540, 1703, 2397
SfoI GGCGCC 2 cut(s) 523, 872
SinI GGWCC 8 cut(s) 233, 262, 556, 2320, 2416, 2768, 3161, 3306
SmiMI CAYNNNNRTG 4 cut(s) 711, 1823, 2193, 2244
SmlI CTYRAG 1 cut(s) 2045
SmoI CTYRAG 1 cut(s) 2045
SseBI AGGCCT 1 cut(s) 1642
SsiI CCGC 9 cut(s) 749, 1221, 1833, 1860, 1877, 1932, 1956, 3293, 3309
SspDI GGCGCC 2 cut(s) 521, 870
SspI AATATT 1 cut(s) 1364
SstI GAGCTC 1 cut(s) 2964
StuI AGGCCT 1 cut(s) 1642
StyI CCWWGG 2 cut(s) 25, 411
TaaI ACNGT 8 cut(s) 259, 353, 1035, 1408, 2096, 2420, 2982, 2990
TaiI ACGT 3 cut(s) 350, 2764, 3235
TaqI TCGA 8 cut(s) 13, 65, 177, 726, 942, 1659, 2288, 3252
TaqII GACCGA 1 cut(s) 544
TatI WGTACW 2 cut(s) 1230, 2325
TauI GCSGC 5 cut(s) 1224, 1835, 1880, 1935, 1958
TfiI GAWTC 9 cut(s) 5, 15, 116, 430, 649, 1623, 2176, 2206, 3134
Tru1I TTAA 9 cut(s) 744, 1431, 1584, 1637, 1718, 1805, 1905, 1940, 2754
Tru9I TTAA 9 cut(s) 744, 1431, 1584, 1637, 1718, 1805, 1905, 1940, 2754
TseFI GTSAC 6 cut(s) 704, 1846, 2306, 2332, 2425, 2914
Tsp45I GTSAC 6 cut(s) 704, 1846, 2306, 2332, 2425, 2914
TspDTI ATGAA 8 cut(s) 1116, 1148, 1664, 2177, 2358, 2498, 2641, 2748
Van91I CCANNNNNTGG 1 cut(s) 2597
VpaK11BI GGWCC 8 cut(s) 233, 262, 556, 2320, 2416, 2768, 3161, 3306
XagI CCTNNNNNAGG 1 cut(s) 892
XapI RAATTY 4 cut(s) 765, 1135, 1683, 3067
XbaI TCTAGA 2 cut(s) 2026, 3257
XceI RCATGY 6 cut(s) 716, 1757, 1939, 1947, 2504, 2683
XmiI GTMKAC 2 cut(s) 1394, 1948
XmnI GAANNNNTTC 2 cut(s) 1655, 3005
ZrmI AGTACT 1 cut(s) 1232
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.