FvH4_2g29190

alpha/beta hydrolase fold

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb2
OG0017993
Physical Location & Seq
Forward (+)
22696220 .. 22697183
964 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_2g29190.t1

Sequence Viewer

Length: 936 bp
ATGTTTATAGTTGAAGAATCACCAGACTTTCTCCAAGTCTTTTCTGATGGAACTGTGAAACGCTTTTCCCCCGGGATAGTCACTGCCTCATCAGAACCCTCCAATGGATACAAGTTCAAGGATGTTGTCATCGACTCGTCGAAACCAATTACAGGGAGGATCTTCATTCCTTGTGATCCAACATCCTCTAGTAAGCTTCCAGTTATGGTTTATTTTCATGGTGGAGGCTTCTGCATTGGCTCAACCACTTGGATTGGTTACCATCATTTCCTAGGAGACTTATCTGTTGCATCGCAATCGATTGTTCTCTCAATTGACTACCGTTTGGCTCCAGAGAACCGGCTTCCAATACCTTATGAAGACTGTTATGAATCACTTGTTTGGCTTCGTCACCAGGCAAGCTGTGATCCATGGCTGAACAAGGCTGACCTCTCCCGGGTTTTCCTATCCGGTGACAGTGCTGGAGGGAACATAGCACACAATGTTGCTGTGAAAACTATGCACAATTCACTCATTCATGTCAAGATAAGAGGACTGTTGTTGATACATCCTTATTTTGGGAGTGAAAGGAGAACTGATAAAGAGATGTCAACAGATGAAGAGGCAGTTGGGAATGTGGCAAGCAATGACATGTTCTGGAGACTCAGTATACCTGAGGGCTCCAATCGCGACTATTTTGGATGTAACTTTGACAAGACAGAACTGTCTCTAACGCAATGGCGCAACGATTTTCCTGCTGTGACAGTTTATGTTGCTGAACTGGATTTTTTGAAAGAAAGAGGTGTAATGTATGCAGAGTTTTTGCAGAGAAAAGGAGTTGAGGTGAAGCTAGTTGAGGCTGAGAACGAGTCCCATGTATTTCATGTTTTCAAACCCGATTGTGAGGCAACCCGTTTGCTTCAGAAAAACATGAATGATTTCATTTGGAACTATTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Abhydrolase_3

Protein Analysis

312

Amino Acids

35.25

Weight (kDa)

5.48

Isoelectric Point (pI)

39.41

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
COesterase PF00135 54 - 110 6.7e-08 Carboxylesterase family
BD-FAE PF20434 60 - 169 5.6e-13 BD-FAE
Abhydrolase_3 PF07859 69 - 288 1e-54 alpha/beta hydrolase fold
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0017993)

Species Orthologous Gene IDs
fragaria_vesca FvH4_2g29190
malus_domestica MD15G1124600.v1.1
prunus_persica Prupe.1G480100_v2.0.a1
pyrus_communis pycom15g11220
rosa_chinensis RchiOBHm_Chr6g0298051
rosa_laevigata RLG00000011540
rosa_multiflora Rmu_sc0002800.1_g000016
rosa_rugosa Rorug06G0278200
rosa_samantha Rh6AG388700 Rh6CG402900
rosa_wichuraiana Rw6G033930

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 703
AccI GTMKAC 1 cut(s) 649
AccII CGCG 1 cut(s) 669
AclWI GGATC 3 cut(s) 167, 170, 401
AcuI CTGAAG 1 cut(s) 884
AfiI CCNNNNNNNGG 4 cut(s) 104, 152, 436, 557
AflIII ACRYGT 1 cut(s) 630
AgsI TTSAA 4 cut(s) 14, 118, 772, 871
AjnI CCWGG 1 cut(s) 393
AjuI GAANNNNNNNTTGG 2 cut(s) 591, 623
AluBI AGCT 3 cut(s) 196, 402, 829
AluI AGCT 3 cut(s) 196, 402, 829
Alw26I GTCTC 3 cut(s) 270, 634, 711
AlwI GGATC 3 cut(s) 167, 170, 401
Ama87I CYCGRG 2 cut(s) 71, 435
Asp700I GAANNNNTTC 1 cut(s) 917
AspA2I CCTAGG 1 cut(s) 271
AspLEI GCGC 1 cut(s) 723
AsuC2I CCSGG 4 cut(s) 72, 73, 436, 437
AsuHPI GGTGA 4 cut(s) 12, 383, 464, 835
AvaI CYCGRG 2 cut(s) 71, 435
AvrII CCTAGG 1 cut(s) 271
AxyI CCTNAGG 1 cut(s) 654
BanII GRGCYC 1 cut(s) 662
BbsI GAAGAC 1 cut(s) 366
BccI CCATC 2 cut(s) 41, 270
BcgI CGANNNNNNTGC 4 cut(s) 279, 313, 716, 750
BciT130I CCWGG 1 cut(s) 395
BciVI GTATCC 1 cut(s) 101
BcnI CCSGG 4 cut(s) 72, 73, 436, 437
BcoDI GTCTC 3 cut(s) 270, 634, 711
BfaI CTAG 3 cut(s) 189, 272, 830
BfuI GTATCC 1 cut(s) 101
BlnI CCTAGG 1 cut(s) 271
Bme1390I CCNGG 5 cut(s) 72, 73, 395, 436, 437
BmeT110I CYCGRG 2 cut(s) 71, 435
BmiI GGNNCC 2 cut(s) 330, 661
BmrFI CCNGG 5 cut(s) 72, 73, 395, 436, 437
BmsI GCATC 1 cut(s) 299
BpiI GAAGAC 1 cut(s) 366
BpmI CTGGAG 3 cut(s) 315, 483, 658
BpuMI CCSGG 4 cut(s) 72, 73, 436, 437
Bsa29I ATCGAT 1 cut(s) 299
BsaJI CCNNGG 5 cut(s) 70, 71, 271, 410, 435
BsaWI WCCGGW 1 cut(s) 449
BsaXI ACNNNNNCTCC 4 cut(s) 631, 661, 807, 837
Bsc4I CCNNNNNNNGG 4 cut(s) 104, 152, 436, 557
Bse118I RCCGGY 1 cut(s) 339
Bse1I ACTGG 2 cut(s) 200, 765
Bse21I CCTNAGG 1 cut(s) 654
Bse3DI GCAATG 2 cut(s) 631, 722
BseBI CCWGG 1 cut(s) 395
BseCI ATCGAT 1 cut(s) 299
BseDI CCNNGG 5 cut(s) 70, 71, 271, 410, 435
BseGI GGATG 4 cut(s) 127, 182, 547, 686
BseLI CCNNNNNNNGG 4 cut(s) 104, 152, 436, 557
BseMI GCAATG 2 cut(s) 631, 722
BseMII CTCAG 3 cut(s) 645, 658, 831
BseNI ACTGG 2 cut(s) 200, 765
Bsh1236I CGCG 1 cut(s) 669
BshVI ATCGAT 1 cut(s) 299
BsiHKCI CYCGRG 2 cut(s) 71, 435
BsiSI CCGG 4 cut(s) 72, 340, 436, 450
BslFI GGGAC 1 cut(s) 835
BslI CCNNNNNNNGG 4 cut(s) 104, 152, 436, 557
BsmAI GTCTC 3 cut(s) 270, 634, 711
BsmFI GGGAC 1 cut(s) 835
BsoBI CYCGRG 2 cut(s) 71, 435
Bsp1286I GDGCHC 1 cut(s) 662
Bsp143I GATC 3 cut(s) 159, 175, 406
Bsp19I CCATGG 1 cut(s) 410
Bsp68I TCGCGA 1 cut(s) 669
BspCNI CTCAG 3 cut(s) 646, 657, 832
BspDI ATCGAT 1 cut(s) 299
BspFNI CGCG 1 cut(s) 669
BspLI GGNNCC 2 cut(s) 330, 661
BspPI GGATC 3 cut(s) 167, 170, 401
BsrDI GCAATG 2 cut(s) 631, 722
BsrFI RCCGGY 1 cut(s) 339
BsrI ACTGG 2 cut(s) 200, 765
BssAI RCCGGY 1 cut(s) 339
BssECI CCNNGG 5 cut(s) 70, 71, 271, 410, 435
BssMI GATC 3 cut(s) 159, 175, 406
BssNAI GTATAC 1 cut(s) 650
BssT1I CCWWGG 2 cut(s) 271, 410
Bst1107I GTATAC 1 cut(s) 650
Bst2UI CCWGG 1 cut(s) 395
Bst4CI ACNGT 7 cut(s) 55, 323, 365, 458, 537, 705, 745
Bst6I CTCTTC 1 cut(s) 594
BstC8I GCNNGC 2 cut(s) 400, 622
BstDEI CTNAG 3 cut(s) 644, 654, 840
BstDSI CCRYGG 1 cut(s) 410
BstEII GGTNACC 1 cut(s) 257
BstF5I GGATG 4 cut(s) 127, 182, 547, 686
BstFNI CGCG 1 cut(s) 669
BstHHI GCGC 1 cut(s) 723
BstKTI GATC 3 cut(s) 162, 178, 409
BstMAI GTCTC 3 cut(s) 270, 634, 711
BstMBI GATC 3 cut(s) 159, 175, 406
BstMWI GCNNNNNNNGC 1 cut(s) 666
BstNI CCWGG 1 cut(s) 395
BstNSI RCATGY 1 cut(s) 634
BstPI GGTNACC 1 cut(s) 257
BstSCI CCNGG 5 cut(s) 70, 71, 393, 434, 435
BstUI CGCG 1 cut(s) 669
BstV2I GAAGAC 1 cut(s) 366
BstX2I RGATCY 1 cut(s) 159
BstYI RGATCY 1 cut(s) 159
BstZ17I GTATAC 1 cut(s) 650
Bsu15I ATCGAT 1 cut(s) 299
Bsu36I CCTNAGG 1 cut(s) 654
BsuI GTATCC 1 cut(s) 101
BsuTUI ATCGAT 1 cut(s) 299
BtgI CCRYGG 1 cut(s) 410
BtgZI GCGATG 1 cut(s) 276
BtsCI GGATG 4 cut(s) 127, 182, 547, 686
BtsI GCAGTG 1 cut(s) 81
BtsIMutI CAGTG 2 cut(s) 81, 463
BtuMI TCGCGA 1 cut(s) 669
Cac8I GCNNGC 2 cut(s) 400, 622
CfoI GCGC 1 cut(s) 723
Cfr10I RCCGGY 1 cut(s) 339
Cfr9I CCCGGG 2 cut(s) 71, 435
ClaI ATCGAT 1 cut(s) 299
CspCI CAANNNNNGTGG 2 cut(s) 235, 270
CviAII CATG 7 cut(s) 218, 411, 518, 631, 854, 863, 910
DdeI CTNAG 3 cut(s) 644, 654, 840
DpnI GATC 3 cut(s) 161, 177, 408
DpnII GATC 3 cut(s) 159, 175, 406
DrdI GACNNNNNNGTC 1 cut(s) 703
DseDI GACNNNNNNGTC 1 cut(s) 703
Eam1104I CTCTTC 1 cut(s) 594
EarI CTCTTC 1 cut(s) 594
Eco130I CCWWGG 2 cut(s) 271, 410
Eco24I GRGCYC 1 cut(s) 662
Eco57I CTGAAG 1 cut(s) 884
Eco81I CCTNAGG 1 cut(s) 654
Eco88I CYCGRG 2 cut(s) 71, 435
Eco91I GGTNACC 1 cut(s) 257
EcoO65I GGTNACC 1 cut(s) 257
EcoRII CCWGG 1 cut(s) 393
EcoT14I CCWWGG 2 cut(s) 271, 410
EcoT38I GRGCYC 1 cut(s) 662
ErhI CCWWGG 2 cut(s) 271, 410
FaeI CATG 7 cut(s) 221, 414, 521, 634, 857, 866, 913
FaqI GGGAC 1 cut(s) 835
FatI CATG 7 cut(s) 217, 410, 517, 630, 853, 862, 909
FblI GTMKAC 1 cut(s) 649
FokI GGATG 4 cut(s) 134, 169, 534, 693
FriOI GRGCYC 1 cut(s) 662
FspBI CTAG 3 cut(s) 189, 272, 830
GlaI GCGC 1 cut(s) 722
GsuI CTGGAG 3 cut(s) 315, 483, 658
HapII CCGG 4 cut(s) 72, 340, 436, 450
HhaI GCGC 1 cut(s) 723
Hin1II CATG 7 cut(s) 221, 414, 521, 634, 857, 866, 913
Hin6I GCGC 1 cut(s) 721
HinP1I GCGC 1 cut(s) 721
HincII GTYRAC 1 cut(s) 591
HindII GTYRAC 1 cut(s) 591
HindIII AAGCTT 1 cut(s) 194
HinfI GANTC 5 cut(s) 17, 134, 371, 642, 848
HpaII CCGG 4 cut(s) 72, 340, 436, 450
HphI GGTGA 4 cut(s) 12, 383, 464, 835
Hpy166II GTNNAC 2 cut(s) 591, 650
Hpy188I TCNGA 3 cut(s) 46, 94, 903
Hpy188III TCNNGA 4 cut(s) 332, 523, 637, 668
Hpy8I GTNNAC 2 cut(s) 591, 650
Hpy99I CGWCG 1 cut(s) 142
HpyCH4III ACNGT 7 cut(s) 55, 323, 365, 458, 537, 705, 745
HpyCH4V TGCA 5 cut(s) 234, 290, 502, 794, 805
HpyF10VI GCNNNNNNNGC 1 cut(s) 666
HpyF3I CTNAG 3 cut(s) 644, 654, 840
Hsp92II CATG 7 cut(s) 221, 414, 521, 634, 857, 866, 913
HspAI GCGC 1 cut(s) 721
Kzo9I GATC 3 cut(s) 159, 175, 406
LmnI GCTCC 2 cut(s) 334, 665
LweI GCATC 1 cut(s) 299
MaeI CTAG 3 cut(s) 189, 272, 830
MaeIII GTNAC 6 cut(s) 79, 257, 389, 452, 683, 739
MalI GATC 3 cut(s) 161, 177, 408
MboI GATC 3 cut(s) 159, 175, 406
MboII GAAGA 4 cut(s) 26, 154, 371, 611
MfeI CAATTG 1 cut(s) 312
MflI RGATCY 1 cut(s) 159
MhlI GDGCHC 1 cut(s) 662
MluCI AATT 3 cut(s) 147, 312, 505
MlyI GAGTC 3 cut(s) 128, 636, 857
MmeI TCCRAC 1 cut(s) 203
MroXI GAANNNNTTC 1 cut(s) 917
MspI CCGG 4 cut(s) 72, 340, 436, 450
MspR9I CCNGG 5 cut(s) 72, 73, 395, 436, 437
MunI CAATTG 1 cut(s) 312
MvaI CCWGG 1 cut(s) 395
MvnI CGCG 1 cut(s) 669
MwoI GCNNNNNNNGC 1 cut(s) 666
NciI CCSGG 4 cut(s) 72, 73, 436, 437
NcoI CCATGG 1 cut(s) 410
NdeII GATC 3 cut(s) 159, 175, 406
NlaIII CATG 7 cut(s) 221, 414, 521, 634, 857, 866, 913
NlaIV GGNNCC 2 cut(s) 330, 661
NmuCI GTSAC 4 cut(s) 79, 389, 452, 739
NruI TCGCGA 1 cut(s) 669
NspI RCATGY 1 cut(s) 634
PciI ACATGT 1 cut(s) 630
PdmI GAANNNNTTC 1 cut(s) 917
PfeI GAWTC 2 cut(s) 17, 371
PleI GAGTC 3 cut(s) 128, 636, 856
PpsI GAGTC 3 cut(s) 128, 636, 856
PscI ACATGT 1 cut(s) 630
Psp6I CCWGG 1 cut(s) 393
PspEI GGTNACC 1 cut(s) 257
PspGI CCWGG 1 cut(s) 393
PspN4I GGNNCC 2 cut(s) 330, 661
PsuI RGATCY 1 cut(s) 159
RruI TCGCGA 1 cut(s) 669
Sau3AI GATC 3 cut(s) 159, 175, 406
SchI GAGTC 3 cut(s) 128, 636, 857
ScrFI CCNGG 5 cut(s) 72, 73, 395, 436, 437
SduI GDGCHC 1 cut(s) 662
SetI ASST 8 cut(s) 198, 355, 404, 432, 655, 784, 825, 831
SfaNI GCATC 1 cut(s) 299
SmaI CCCGGG 2 cut(s) 73, 437
Sse9I AATT 3 cut(s) 147, 312, 505
SspMI CTAG 3 cut(s) 189, 272, 830
StyD4I CCNGG 5 cut(s) 70, 71, 393, 434, 435
StyI CCWWGG 2 cut(s) 271, 410
TaaI ACNGT 7 cut(s) 55, 323, 365, 458, 537, 705, 745
TaqI TCGA 3 cut(s) 132, 140, 299
TasI AATT 3 cut(s) 147, 312, 505
TfiI GAWTC 2 cut(s) 17, 371
TscAI CASTG 2 cut(s) 88, 463
TseFI GTSAC 4 cut(s) 79, 389, 452, 739
Tsp45I GTSAC 4 cut(s) 79, 389, 452, 739
TspDTI ATGAA 9 cut(s) 154, 206, 372, 384, 506, 612, 851, 910, 926
TspMI CCCGGG 2 cut(s) 71, 435
TspRI CASTG 2 cut(s) 88, 463
XceI RCATGY 1 cut(s) 634
XmaI CCCGGG 2 cut(s) 71, 435
XmaJI CCTAGG 1 cut(s) 271
XmiI GTMKAC 1 cut(s) 649
XmnI GAANNNNTTC 1 cut(s) 917
XspI CTAG 3 cut(s) 189, 272, 830
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.