pycom15g11220

alpha/beta hydrolase fold

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr15
OG0017993
Physical Location & Seq
Reverse (-)
7576512 .. 7577444
933 bp
Loading structure...
UTR
Exon/CDS
Intron
pycom15g11220.1

Sequence Viewer

Length: 933 bp
ATGTCCATTGTTGAAGAAGCACCGGACTTTCTTCAAGTTTTTTCCGATGGATCTGTAAAACGCTTTTCACCCGGGGTGGCATCTGCCTCACCAGAATCAACCGACGGATTCAAGTCCAAGGATGTGATTATTGACTCATCGAAACCCATTACGGGAAGGATTTTCCTTCCTGGTAATCCAACATCTTCGAAGAAGCTTCCGGTTGTGGTTAATTTTCACGGTGGTGGCTTCTGCATTGGCTCAACCACATGGCTTGGCTACCATCATTTCTTAGGAGGCTTAGCTGTTGCATCCCAATCCATTGTTGTGTCCGTTGACTATCGTTTAGCTCCAGAGAACCGGCTCCCCGTAGCTTATGAAGACTGCTATTACACGTTCGATTGGCTTAGTCGCCAAGCAAGCTCTGAACCATGGCTAGACAAAGCTGATCTCTCTCGGGTTTTTCTCACCGGGGACAGCGCTGGAGGGAACATAACTCACAATGTTGCTGTGAAAGCCATTTGCAATCGAATCGGTCATGTCAAAATAAGGGGATTATTGTTGGTACATCCTTATTTTGGGAGTGAAAAGAGAACCGAGAAAGAGATGGCTGAAGAGGGAGCAAAGGATGTGACGAGCAATGACATGTTTTGGCGACTAAGCATACCTAAAGGCTCCAACCGAGACTATTTCGGATGTAACTTTGAAAAGACCGGACTGTCTGCAACCGAATGGAGCGATGAGTTCCCTGCTGTGGTTGTTTATGTGGCTGGCTTGGATTTTTTGAAAGAGAGGGGAGTGATGTATGCAGATTTTCTGCAGAAAAAAGGAGTTAAAGAAGTGAAGCTGGTGGAGGCGGAGAACGAGTCTCATGTGTTTCATGTCTTCCATCCAGATTCAGAGGGGGCCGGTTTGCTTCAGAGAAATATGGGTGAATTCATCAGGAGCCATTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Abhydrolase_3

Protein Analysis

311

Amino Acids

34.25

Weight (kDa)

5.94

Isoelectric Point (pI)

38.03

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
COesterase PF00135 54 - 110 7.5e-07 Carboxylesterase family
BD-FAE PF20434 60 - 165 3.6e-12 BD-FAE
Abhydrolase_3 PF07859 69 - 287 3.6e-51 alpha/beta hydrolase fold
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0017993)

Species Orthologous Gene IDs
fragaria_vesca FvH4_2g29190
malus_domestica MD15G1124600.v1.1
prunus_persica Prupe.1G480100_v2.0.a1
pyrus_communis pycom15g11220
rosa_chinensis RchiOBHm_Chr6g0298051
rosa_laevigata RLG00000011540
rosa_multiflora Rmu_sc0002800.1_g000016
rosa_rugosa Rorug06G0278200
rosa_samantha Rh6AG388700 Rh6CG402900
rosa_wichuraiana Rw6G033930

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 697
AciI CCGC 1 cut(s) 836
AclWI GGATC 1 cut(s) 58
AcsI RAATTY 1 cut(s) 914
AcuI CTGAAG 2 cut(s) 612, 881
AfaI GTAC 1 cut(s) 546
AfeI AGCGCT 1 cut(s) 460
AfiI CCNNNNNNNGG 3 cut(s) 152, 557, 733
AflIII ACRYGT 2 cut(s) 372, 624
AgsI TTSAA 5 cut(s) 14, 35, 112, 686, 766
AjnI CCWGG 1 cut(s) 169
AleI CACNNNNGTG 1 cut(s) 222
AluBI AGCT 7 cut(s) 196, 284, 329, 353, 402, 425, 826
AluI AGCT 7 cut(s) 196, 284, 329, 353, 402, 425, 826
Alw26I GTCTC 2 cut(s) 657, 852
AlwI GGATC 1 cut(s) 58
Ama87I CYCGRG 2 cut(s) 71, 435
Aor51HI AGCGCT 1 cut(s) 460
AoxI GGCC 1 cut(s) 885
ApoI RAATTY 1 cut(s) 914
AspLEI GCGC 1 cut(s) 461
AspS9I GGNCC 1 cut(s) 885
AsuC2I CCSGG 3 cut(s) 72, 73, 451
AsuHPI GGTGA 4 cut(s) 60, 81, 439, 923
AsuII TTCGAA 1 cut(s) 188
AvaI CYCGRG 2 cut(s) 71, 435
BbsI GAAGAC 2 cut(s) 366, 856
BccI CCATC 4 cut(s) 41, 270, 580, 876
BcgI CGANNNNNNTGC 2 cut(s) 493, 527
BciT130I CCWGG 1 cut(s) 171
BcnI CCSGG 3 cut(s) 72, 73, 451
BcoDI GTCTC 2 cut(s) 657, 852
BfaI CTAG 1 cut(s) 416
BfmI CTRYAG 1 cut(s) 797
BfoI RGCGCY 1 cut(s) 462
BlpI GCTNAGC 1 cut(s) 280
Bme1390I CCNGG 4 cut(s) 72, 73, 171, 451
BmeT110I CYCGRG 2 cut(s) 71, 435
BmgT120I GGNCC 1 cut(s) 885
BmiI GGNNCC 4 cut(s) 344, 655, 886, 926
BmrFI CCNGG 4 cut(s) 72, 73, 171, 451
BmsI GCATC 2 cut(s) 89, 299
BpiI GAAGAC 2 cut(s) 366, 856
BpmI CTGGAG 2 cut(s) 315, 483
Bpu1102I GCTNAGC 1 cut(s) 280
Bpu14I TTCGAA 1 cut(s) 188
BpuMI CCSGG 3 cut(s) 72, 73, 451
BsaJI CCNNGG 5 cut(s) 71, 72, 117, 410, 450
BsaWI WCCGGW 3 cut(s) 22, 199, 692
BsaXI ACNNNNNCTCC 2 cut(s) 830, 860
Bsc4I CCNNNNNNNGG 3 cut(s) 152, 557, 733
Bse118I RCCGGY 2 cut(s) 339, 887
Bse3DI GCAATG 1 cut(s) 625
BseBI CCWGG 1 cut(s) 171
BseDI CCNNGG 5 cut(s) 71, 72, 117, 410, 450
BseGI GGATG 6 cut(s) 127, 290, 547, 613, 680, 868
BseLI CCNNNNNNNGG 3 cut(s) 152, 557, 733
BseMI GCAATG 1 cut(s) 625
BshFI GGCC 1 cut(s) 887
BsiHKCI CYCGRG 2 cut(s) 71, 435
BsiSI CCGG 7 cut(s) 23, 72, 200, 340, 450, 693, 888
BslFI GGGAC 1 cut(s) 467
BslI CCNNNNNNNGG 3 cut(s) 152, 557, 733
BsmAI GTCTC 2 cut(s) 657, 852
BsmFI GGGAC 1 cut(s) 467
BsnI GGCC 1 cut(s) 887
BsoBI CYCGRG 2 cut(s) 71, 435
Bsp119I TTCGAA 1 cut(s) 188
Bsp143I GATC 2 cut(s) 50, 427
Bsp1720I GCTNAGC 1 cut(s) 280
Bsp19I CCATGG 1 cut(s) 410
BspACI CCGC 1 cut(s) 836
BspANI GGCC 1 cut(s) 887
BspLI GGNNCC 4 cut(s) 344, 655, 886, 926
BspMAI CTGCAG 1 cut(s) 801
BspPI GGATC 1 cut(s) 58
BspT104I TTCGAA 1 cut(s) 188
BsrDI GCAATG 1 cut(s) 625
BsrFI RCCGGY 2 cut(s) 339, 887
BssAI RCCGGY 2 cut(s) 339, 887
BssECI CCNNGG 5 cut(s) 71, 72, 117, 410, 450
BssMI GATC 2 cut(s) 50, 427
BssT1I CCWWGG 2 cut(s) 117, 410
Bst2UI CCWGG 1 cut(s) 171
Bst4CI ACNGT 2 cut(s) 221, 699
Bst6I CTCTTC 1 cut(s) 588
BstBI TTCGAA 1 cut(s) 188
BstC8I GCNNGC 2 cut(s) 400, 751
BstDEI CTNAG 4 cut(s) 271, 280, 386, 638
BstDSI CCRYGG 1 cut(s) 410
BstF5I GGATG 6 cut(s) 127, 290, 547, 613, 680, 868
BstH2I RGCGCY 1 cut(s) 462
BstHHI GCGC 1 cut(s) 461
BstKTI GATC 2 cut(s) 53, 430
BstMAI GTCTC 2 cut(s) 657, 852
BstMBI GATC 2 cut(s) 50, 427
BstMWI GCNNNNNNNGC 2 cut(s) 399, 494
BstNI CCWGG 1 cut(s) 171
BstNSI RCATGY 1 cut(s) 628
BstSCI CCNGG 4 cut(s) 70, 71, 169, 449
BstSFI CTRYAG 1 cut(s) 797
BstV2I GAAGAC 2 cut(s) 366, 856
BstX2I RGATCY 1 cut(s) 50
BstYI RGATCY 1 cut(s) 50
BsuRI GGCC 1 cut(s) 887
BtgI CCRYGG 1 cut(s) 410
BtgZI GCGATG 1 cut(s) 732
BtsCI GGATG 6 cut(s) 127, 290, 547, 613, 680, 868
Cac8I GCNNGC 2 cut(s) 400, 751
CfoI GCGC 1 cut(s) 461
Cfr10I RCCGGY 2 cut(s) 339, 887
Cfr13I GGNCC 1 cut(s) 885
Cfr9I CCCGGG 1 cut(s) 71
Csp6I GTAC 1 cut(s) 545
CspCI CAANNNNNGTGG 2 cut(s) 235, 270
CviAII CATG 6 cut(s) 249, 411, 518, 625, 851, 860
CviQI GTAC 1 cut(s) 545
DdeI CTNAG 4 cut(s) 271, 280, 386, 638
DpnI GATC 2 cut(s) 52, 429
DpnII GATC 2 cut(s) 50, 427
DrdI GACNNNNNNGTC 1 cut(s) 697
DseDI GACNNNNNNGTC 1 cut(s) 697
Eam1104I CTCTTC 1 cut(s) 588
EarI CTCTTC 1 cut(s) 588
EciI GGCGGA 1 cut(s) 851
Eco130I CCWWGG 2 cut(s) 117, 410
Eco47III AGCGCT 1 cut(s) 460
Eco57I CTGAAG 2 cut(s) 612, 881
Eco88I CYCGRG 2 cut(s) 71, 435
EcoRI GAATTC 1 cut(s) 914
EcoRII CCWGG 1 cut(s) 169
EcoT14I CCWWGG 2 cut(s) 117, 410
ErhI CCWWGG 2 cut(s) 117, 410
FaeI CATG 6 cut(s) 252, 414, 521, 628, 854, 863
FaqI GGGAC 1 cut(s) 467
FatI CATG 6 cut(s) 248, 410, 517, 624, 850, 859
FokI GGATG 6 cut(s) 134, 277, 534, 620, 687, 855
FspBI CTAG 1 cut(s) 416
GlaI GCGC 1 cut(s) 460
GsuI CTGGAG 2 cut(s) 315, 483
HaeII RGCGCY 1 cut(s) 462
HaeIII GGCC 1 cut(s) 887
HapII CCGG 7 cut(s) 23, 72, 200, 340, 450, 693, 888
HhaI GCGC 1 cut(s) 461
Hin1II CATG 6 cut(s) 252, 414, 521, 628, 854, 863
Hin6I GCGC 1 cut(s) 459
HinP1I GCGC 1 cut(s) 459
HincII GTYRAC 1 cut(s) 316
HindII GTYRAC 1 cut(s) 316
HindIII AAGCTT 1 cut(s) 194
HinfI GANTC 6 cut(s) 95, 108, 134, 510, 845, 875
HpaII CCGG 7 cut(s) 23, 72, 200, 340, 450, 693, 888
HphI GGTGA 4 cut(s) 60, 81, 439, 923
Hpy166II GTNNAC 1 cut(s) 316
Hpy188I TCNGA 5 cut(s) 46, 406, 674, 880, 900
Hpy188III TCNNGA 3 cut(s) 332, 872, 922
Hpy8I GTNNAC 1 cut(s) 316
Hpy99I CGWCG 1 cut(s) 107
HpyAV CCTTC 2 cut(s) 150, 176
HpyCH4III ACNGT 2 cut(s) 221, 699
HpyCH4IV ACGT 1 cut(s) 374
HpyCH4V TGCA 6 cut(s) 234, 290, 504, 704, 788, 799
HpyF10VI GCNNNNNNNGC 2 cut(s) 399, 494
HpyF3I CTNAG 4 cut(s) 271, 280, 386, 638
HpySE526I ACGT 1 cut(s) 374
Hsp92II CATG 6 cut(s) 252, 414, 521, 628, 854, 863
HspAI GCGC 1 cut(s) 459
Kzo9I GATC 2 cut(s) 50, 427
LmnI GCTCC 6 cut(s) 334, 348, 599, 659, 714, 924
LweI GCATC 2 cut(s) 89, 299
MaeI CTAG 1 cut(s) 416
MaeII ACGT 1 cut(s) 374
MaeIII GTNAC 2 cut(s) 610, 677
MalI GATC 2 cut(s) 52, 429
MboI GATC 2 cut(s) 50, 427
MboII GAAGA 7 cut(s) 23, 26, 177, 202, 371, 605, 856
MflI RGATCY 1 cut(s) 50
MluCI AATT 2 cut(s) 211, 914
MlyI GAGTC 2 cut(s) 128, 854
MmeI TCCRAC 2 cut(s) 203, 681
MnlI CCTC 7 cut(s) 97, 269, 458, 589, 765, 826, 874
MseI TTAA 2 cut(s) 210, 813
MslI CAYNNNNRTG 2 cut(s) 222, 305
MspI CCGG 7 cut(s) 23, 72, 200, 340, 450, 693, 888
MspR9I CCNGG 4 cut(s) 72, 73, 171, 451
MvaI CCWGG 1 cut(s) 171
MwoI GCNNNNNNNGC 2 cut(s) 399, 494
NciI CCSGG 3 cut(s) 72, 73, 451
NcoI CCATGG 1 cut(s) 410
NdeII GATC 2 cut(s) 50, 427
NlaIII CATG 6 cut(s) 252, 414, 521, 628, 854, 863
NlaIV GGNNCC 4 cut(s) 344, 655, 886, 926
NmuCI GTSAC 1 cut(s) 610
NspI RCATGY 1 cut(s) 628
NspV TTCGAA 1 cut(s) 188
OliI CACNNNNGTG 1 cut(s) 222
PciI ACATGT 1 cut(s) 624
PfeI GAWTC 4 cut(s) 95, 108, 510, 875
PleI GAGTC 2 cut(s) 128, 853
PpsI GAGTC 2 cut(s) 128, 853
PscI ACATGT 1 cut(s) 624
Psp6I CCWGG 1 cut(s) 169
PspGI CCWGG 1 cut(s) 169
PspN4I GGNNCC 4 cut(s) 344, 655, 886, 926
PspPI GGNCC 1 cut(s) 885
PstI CTGCAG 1 cut(s) 801
PsuI RGATCY 1 cut(s) 50
RsaI GTAC 1 cut(s) 546
RsaNI GTAC 1 cut(s) 545
RseI CAYNNNNRTG 2 cut(s) 222, 305
SaqAI TTAA 2 cut(s) 210, 813
Sau3AI GATC 2 cut(s) 50, 427
Sau96I GGNCC 1 cut(s) 885
SchI GAGTC 2 cut(s) 128, 854
ScrFI CCNGG 4 cut(s) 72, 73, 171, 451
SetI ASST 9 cut(s) 198, 286, 331, 355, 377, 404, 427, 649, 828
SfaNI GCATC 2 cut(s) 89, 299
SfcI CTRYAG 1 cut(s) 797
SfuI TTCGAA 1 cut(s) 188
SmaI CCCGGG 1 cut(s) 73
SmiMI CAYNNNNRTG 2 cut(s) 222, 305
Sse9I AATT 2 cut(s) 211, 914
SsiI CCGC 1 cut(s) 836
SspMI CTAG 1 cut(s) 416
StyD4I CCNGG 4 cut(s) 70, 71, 169, 449
StyI CCWWGG 2 cut(s) 117, 410
TaaI ACNGT 2 cut(s) 221, 699
TaiI ACGT 1 cut(s) 377
TaqI TCGA 4 cut(s) 140, 188, 378, 508
TaqII GACCGA 1 cut(s) 503
TasI AATT 2 cut(s) 211, 914
TfiI GAWTC 4 cut(s) 95, 108, 510, 875
Tru1I TTAA 2 cut(s) 210, 813
Tru9I TTAA 2 cut(s) 210, 813
TseFI GTSAC 1 cut(s) 610
Tsp45I GTSAC 1 cut(s) 610
TspDTI ATGAA 3 cut(s) 372, 848, 907
TspGWI ACGGA 2 cut(s) 120, 301
TspMI CCCGGG 1 cut(s) 71
XapI RAATTY 1 cut(s) 914
XceI RCATGY 1 cut(s) 628
XmaI CCCGGG 1 cut(s) 71
XspI CTAG 1 cut(s) 416
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.