Rw6G033930

alpha/beta hydrolase fold

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_wichuraiana
Chr6
OG0017993
Physical Location & Seq
Forward (+)
58492192 .. 58493127
936 bp
Loading structure...
UTR
Exon/CDS
Intron
Rw6G033930.1

Sequence Viewer

Length: 936 bp
ATGTCTTTAGTTGAAGAATCACCAGACTTTCTCCAAGTCTTTTCTGATGGAACTGTGAAACGCTTTGCACCCGGAATAATCCCTGCCTCATCAGAACCATCTAATGGATACAAGTTCAAGGATGTAATCATTGACTCGTCGAAACCAATTACTGGGAGGGTTTTCATACCTAGTGATCCAACATCCTTGAGTAAGCTTCCAGTTGTGGTTTATTTTCATGGTGGAGGCTTCTGCATCGGCTCAACCACATGGATTGGTTACCATCATTTCCTAGGAGACTTATCCGTTTCATCCCAATCGATTGTTCTTTCAATTGACTACCGTTTGGCTCCAGAGAACCGGCTTCCCATAGCTTATGAAGACTGTTATGACTCACTTGTTTGGCTTGGTCACCAAGCAAGCTGTGATCCATGGCTGAACAAGGCTGACCTCTCCCGGGTTTTCTTATCCGGGGACAGTGCTGGTGGGAACATAGCACACAATGTTGCTGTGAAAACTATGCGCAATTCACTCATTCATGTCAAGATACGAGGACTGTTGTTGATACATCCTTATTTTGGGAGTGAAAAGAGAACTGAGAAAGAGATGTCGACGGATCAAGAGGCAGTTGGGAATGTGGCAAGCAATGACATGTTCTGGAGACTTGGTATACCTGAAGGTTCCAATCGCGATTATTTCGGATGCAACTTTGACAAGACAGAACTGTCTGTAATCCAATGGCGCAATGAGTTTCCTGCTGTGACAGTTTATGTTGCTGAACTGGATTTTTTGAAAGAGAGGGGTGTAATGTATGCAGAGTTTCTGCAAAGAAAAGGAGTTGAAGTGAAGCTGGTCGAGGCTGAGAATGAGTCCCATGTGTTTCATGTATTCAAACCTGAATGTGAGGCAACCCGTTTGCTTCAGAAAAACATGAAGGAGTTCATTTGGATCTATTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Abhydrolase_3

Protein Analysis

311

Amino Acids

35.06

Weight (kDa)

5.63

Isoelectric Point (pI)

40.35

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
COesterase PF00135 54 - 110 4.8e-07 Carboxylesterase family
BD-FAE PF20434 62 - 169 1.2e-12 BD-FAE
Abhydrolase_3 PF07859 69 - 288 1.5e-54 alpha/beta hydrolase fold
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0017993)

Species Orthologous Gene IDs
fragaria_vesca FvH4_2g29190
malus_domestica MD15G1124600.v1.1
prunus_persica Prupe.1G480100_v2.0.a1
pyrus_communis pycom15g11220
rosa_chinensis RchiOBHm_Chr6g0298051
rosa_laevigata RLG00000011540
rosa_multiflora Rmu_sc0002800.1_g000016
rosa_rugosa Rorug06G0278200
rosa_samantha Rh6AG388700 Rh6CG402900
rosa_wichuraiana Rw6G033930

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 703
Acc16I TGCGCA 1 cut(s) 503
AccB7I CCANNNNNTGG 2 cut(s) 104, 152
AccI GTMKAC 2 cut(s) 590, 649
AccII CGCG 1 cut(s) 669
AclWI GGATC 4 cut(s) 170, 401, 603, 935
AcuI CTGAAG 2 cut(s) 675, 884
AfiI CCNNNNNNNGG 4 cut(s) 104, 152, 436, 557
AflIII ACRYGT 1 cut(s) 630
AgsI TTSAA 6 cut(s) 14, 118, 312, 772, 821, 871
AjuI GAANNNNNNNTTGG 2 cut(s) 288, 320
AluBI AGCT 4 cut(s) 196, 353, 402, 829
AluI AGCT 4 cut(s) 196, 353, 402, 829
Alw26I GTCTC 2 cut(s) 270, 634
AlwI GGATC 4 cut(s) 170, 401, 603, 935
Ama87I CYCGRG 1 cut(s) 435
Asp700I GAANNNNTTC 1 cut(s) 917
AspA2I CCTAGG 1 cut(s) 271
AspLEI GCGC 2 cut(s) 504, 723
AsuC2I CCSGG 4 cut(s) 72, 436, 437, 451
AsuHPI GGTGA 2 cut(s) 12, 383
AvaI CYCGRG 1 cut(s) 435
AvrII CCTAGG 1 cut(s) 271
BbsI GAAGAC 1 cut(s) 366
BccI CCATC 3 cut(s) 41, 106, 270
BciVI GTATCC 1 cut(s) 101
BcnI CCSGG 4 cut(s) 72, 436, 437, 451
BcoDI GTCTC 2 cut(s) 270, 634
BfaI CTAG 2 cut(s) 171, 272
BfuI GTATCC 1 cut(s) 101
BlnI CCTAGG 1 cut(s) 271
Bme1390I CCNGG 4 cut(s) 72, 436, 437, 451
BmeT110I CYCGRG 1 cut(s) 435
BmiI GGNNCC 2 cut(s) 330, 661
BmrFI CCNGG 4 cut(s) 72, 436, 437, 451
BmrI ACTGGG 1 cut(s) 162
BmsI GCATC 2 cut(s) 243, 671
BmuI ACTGGG 1 cut(s) 162
BpiI GAAGAC 1 cut(s) 366
BpmI CTGGAG 2 cut(s) 315, 658
BpuEI CTTGAG 1 cut(s) 208
BpuMI CCSGG 4 cut(s) 72, 436, 437, 451
Bsa29I ATCGAT 1 cut(s) 299
BsaJI CCNNGG 4 cut(s) 271, 410, 435, 450
BsaXI ACNNNNNCTCC 4 cut(s) 631, 661, 807, 837
Bsc4I CCNNNNNNNGG 4 cut(s) 104, 152, 436, 557
Bse118I RCCGGY 1 cut(s) 339
Bse1I ACTGG 3 cut(s) 157, 200, 765
Bse3DI GCAATG 2 cut(s) 631, 730
BseCI ATCGAT 1 cut(s) 299
BseDI CCNNGG 4 cut(s) 271, 410, 435, 450
BseGI GGATG 5 cut(s) 127, 182, 290, 547, 686
BseLI CCNNNNNNNGG 4 cut(s) 104, 152, 436, 557
BseMI GCAATG 2 cut(s) 631, 730
BseMII CTCAG 2 cut(s) 567, 831
BseNI ACTGG 3 cut(s) 157, 200, 765
Bsh1236I CGCG 1 cut(s) 669
BshVI ATCGAT 1 cut(s) 299
BsiHKCI CYCGRG 1 cut(s) 435
BsiSI CCGG 4 cut(s) 72, 340, 436, 450
BslFI GGGAC 2 cut(s) 467, 835
BslI CCNNNNNNNGG 4 cut(s) 104, 152, 436, 557
BsmAI GTCTC 2 cut(s) 270, 634
BsmFI GGGAC 2 cut(s) 467, 835
BsoBI CYCGRG 1 cut(s) 435
Bsp143I GATC 4 cut(s) 175, 406, 595, 927
Bsp19I CCATGG 1 cut(s) 410
Bsp68I TCGCGA 1 cut(s) 669
BspCNI CTCAG 2 cut(s) 568, 832
BspDI ATCGAT 1 cut(s) 299
BspFNI CGCG 1 cut(s) 669
BspLI GGNNCC 2 cut(s) 330, 661
BspPI GGATC 4 cut(s) 170, 401, 603, 935
BsrDI GCAATG 2 cut(s) 631, 730
BsrFI RCCGGY 1 cut(s) 339
BsrI ACTGG 3 cut(s) 157, 200, 765
BssAI RCCGGY 1 cut(s) 339
BssECI CCNNGG 4 cut(s) 271, 410, 435, 450
BssMI GATC 4 cut(s) 175, 406, 595, 927
BssNAI GTATAC 1 cut(s) 650
BssT1I CCWWGG 2 cut(s) 271, 410
Bst1107I GTATAC 1 cut(s) 650
Bst4CI ACNGT 7 cut(s) 55, 323, 365, 458, 537, 705, 745
BstC8I GCNNGC 2 cut(s) 400, 622
BstDEI CTNAG 2 cut(s) 576, 840
BstDSI CCRYGG 1 cut(s) 410
BstEII GGTNACC 2 cut(s) 257, 389
BstF5I GGATG 5 cut(s) 127, 182, 290, 547, 686
BstFNI CGCG 1 cut(s) 669
BstHHI GCGC 2 cut(s) 504, 723
BstKTI GATC 4 cut(s) 178, 409, 598, 930
BstMAI GTCTC 2 cut(s) 270, 634
BstMBI GATC 4 cut(s) 175, 406, 595, 927
BstNSI RCATGY 1 cut(s) 634
BstPI GGTNACC 2 cut(s) 257, 389
BstSCI CCNGG 4 cut(s) 70, 434, 435, 449
BstUI CGCG 1 cut(s) 669
BstV2I GAAGAC 1 cut(s) 366
BstX2I RGATCY 1 cut(s) 927
BstYI RGATCY 1 cut(s) 927
BstZ17I GTATAC 1 cut(s) 650
Bsu15I ATCGAT 1 cut(s) 299
BsuI GTATCC 1 cut(s) 101
BsuTUI ATCGAT 1 cut(s) 299
BtgI CCRYGG 1 cut(s) 410
BtsCI GGATG 5 cut(s) 127, 182, 290, 547, 686
BtsIMutI CAGTG 1 cut(s) 463
BtuMI TCGCGA 1 cut(s) 669
Cac8I GCNNGC 2 cut(s) 400, 622
CfoI GCGC 2 cut(s) 504, 723
Cfr10I RCCGGY 1 cut(s) 339
Cfr9I CCCGGG 1 cut(s) 435
ClaI ATCGAT 1 cut(s) 299
CspCI CAANNNNNGTGG 2 cut(s) 235, 270
CviAII CATG 8 cut(s) 218, 249, 411, 518, 631, 854, 863, 910
DdeI CTNAG 2 cut(s) 576, 840
DpnI GATC 4 cut(s) 177, 408, 597, 929
DpnII GATC 4 cut(s) 175, 406, 595, 927
DrdI GACNNNNNNGTC 1 cut(s) 703
DseDI GACNNNNNNGTC 1 cut(s) 703
Eco130I CCWWGG 2 cut(s) 271, 410
Eco57I CTGAAG 2 cut(s) 675, 884
Eco88I CYCGRG 1 cut(s) 435
Eco91I GGTNACC 2 cut(s) 257, 389
EcoO65I GGTNACC 2 cut(s) 257, 389
EcoT14I CCWWGG 2 cut(s) 271, 410
ErhI CCWWGG 2 cut(s) 271, 410
FaeI CATG 8 cut(s) 221, 252, 414, 521, 634, 857, 866, 913
FaqI GGGAC 2 cut(s) 467, 835
FatI CATG 8 cut(s) 217, 248, 410, 517, 630, 853, 862, 909
FblI GTMKAC 2 cut(s) 590, 649
FokI GGATG 5 cut(s) 134, 169, 277, 534, 693
FspBI CTAG 2 cut(s) 171, 272
FspI TGCGCA 1 cut(s) 503
GlaI GCGC 2 cut(s) 503, 722
GsuI CTGGAG 2 cut(s) 315, 658
HapII CCGG 4 cut(s) 72, 340, 436, 450
HhaI GCGC 2 cut(s) 504, 723
Hin1II CATG 8 cut(s) 221, 252, 414, 521, 634, 857, 866, 913
Hin6I GCGC 2 cut(s) 502, 721
HinP1I GCGC 2 cut(s) 502, 721
HincII GTYRAC 1 cut(s) 591
HindII GTYRAC 1 cut(s) 591
HindIII AAGCTT 1 cut(s) 194
HinfI GANTC 4 cut(s) 17, 134, 371, 848
HpaII CCGG 4 cut(s) 72, 340, 436, 450
HphI GGTGA 2 cut(s) 12, 383
Hpy166II GTNNAC 2 cut(s) 591, 650
Hpy188I TCNGA 4 cut(s) 46, 94, 680, 903
Hpy188III TCNNGA 5 cut(s) 332, 523, 599, 637, 668
Hpy8I GTNNAC 2 cut(s) 591, 650
Hpy99I CGWCG 2 cut(s) 142, 595
HpyAV CCTTC 2 cut(s) 650, 907
HpyCH4III ACNGT 7 cut(s) 55, 323, 365, 458, 537, 705, 745
HpyCH4V TGCA 5 cut(s) 68, 234, 684, 794, 805
HpyF3I CTNAG 2 cut(s) 576, 840
Hsp92II CATG 8 cut(s) 221, 252, 414, 521, 634, 857, 866, 913
HspAI GCGC 2 cut(s) 502, 721
Kzo9I GATC 4 cut(s) 175, 406, 595, 927
LmnI GCTCC 1 cut(s) 334
LweI GCATC 2 cut(s) 243, 671
MaeI CTAG 2 cut(s) 171, 272
MaeIII GTNAC 3 cut(s) 257, 389, 739
MalI GATC 4 cut(s) 177, 408, 597, 929
MboI GATC 4 cut(s) 175, 406, 595, 927
MboII GAAGA 2 cut(s) 26, 371
MfeI CAATTG 1 cut(s) 312
MflI RGATCY 1 cut(s) 927
MluCI AATT 3 cut(s) 147, 312, 505
MlyI GAGTC 3 cut(s) 128, 365, 857
MmeI TCCRAC 1 cut(s) 203
MnlI CCTC 9 cut(s) 97, 150, 218, 440, 524, 595, 771, 829, 877
MroXI GAANNNNTTC 1 cut(s) 917
MspI CCGG 4 cut(s) 72, 340, 436, 450
MspR9I CCNGG 4 cut(s) 72, 436, 437, 451
MunI CAATTG 1 cut(s) 312
MvnI CGCG 1 cut(s) 669
NciI CCSGG 4 cut(s) 72, 436, 437, 451
NcoI CCATGG 1 cut(s) 410
NdeII GATC 4 cut(s) 175, 406, 595, 927
NlaIII CATG 8 cut(s) 221, 252, 414, 521, 634, 857, 866, 913
NlaIV GGNNCC 2 cut(s) 330, 661
NmuCI GTSAC 2 cut(s) 389, 739
NruI TCGCGA 1 cut(s) 669
NsbI TGCGCA 1 cut(s) 503
NspI RCATGY 1 cut(s) 634
PciI ACATGT 1 cut(s) 630
PdmI GAANNNNTTC 1 cut(s) 917
PfeI GAWTC 1 cut(s) 17
PflMI CCANNNNNTGG 2 cut(s) 104, 152
PleI GAGTC 3 cut(s) 128, 365, 856
PpsI GAGTC 3 cut(s) 128, 365, 856
PscI ACATGT 1 cut(s) 630
PspEI GGTNACC 2 cut(s) 257, 389
PspN4I GGNNCC 2 cut(s) 330, 661
PsuI RGATCY 1 cut(s) 927
RruI TCGCGA 1 cut(s) 669
SalI GTCGAC 1 cut(s) 589
Sau3AI GATC 4 cut(s) 175, 406, 595, 927
SchI GAGTC 3 cut(s) 128, 365, 857
ScrFI CCNGG 4 cut(s) 72, 436, 437, 451
SetI ASST 9 cut(s) 172, 198, 355, 404, 432, 655, 661, 831, 877
SfaNI GCATC 2 cut(s) 243, 671
SmaI CCCGGG 1 cut(s) 437
SmlI CTYRAG 1 cut(s) 187
SmoI CTYRAG 1 cut(s) 187
Sse9I AATT 3 cut(s) 147, 312, 505
SspMI CTAG 2 cut(s) 171, 272
StyD4I CCNGG 4 cut(s) 70, 434, 435, 449
StyI CCWWGG 2 cut(s) 271, 410
TaaI ACNGT 7 cut(s) 55, 323, 365, 458, 537, 705, 745
TaqI TCGA 4 cut(s) 140, 299, 590, 834
TasI AATT 3 cut(s) 147, 312, 505
TfiI GAWTC 1 cut(s) 17
TscAI CASTG 1 cut(s) 463
TseFI GTSAC 2 cut(s) 389, 739
Tsp45I GTSAC 2 cut(s) 389, 739
TspDTI ATGAA 8 cut(s) 154, 206, 279, 372, 506, 851, 910, 926
TspGWI ACGGA 2 cut(s) 274, 608
TspMI CCCGGG 1 cut(s) 435
TspRI CASTG 1 cut(s) 463
Van91I CCANNNNNTGG 2 cut(s) 104, 152
XceI RCATGY 1 cut(s) 634
XmaI CCCGGG 1 cut(s) 435
XmaJI CCTAGG 1 cut(s) 271
XmiI GTMKAC 2 cut(s) 590, 649
XmnI GAANNNNTTC 1 cut(s) 917
XspI CTAG 2 cut(s) 171, 272
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.