Basic Information

Type: gene

Biological Identity

Species

fragaria_vesca

Chromosome

Fvb2

Orthogroup ID

OG0008557

Physical Location & Seq

Genomic Coordinates Reverse (-)

23874639 .. 23878291

Gene Structure 3653 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

FvH4_2g30990.t4

Sequence Viewer

CDS Protein Promoter (2k)

Length: 1563 bp

ATGATAGCTTACCTGGAGTGCTTCGTCCAAGAAAATGAAAACGACGCCGAATCCCGGCCATACGACGTCGTCTCCGGCCTCCGCGGGGCCCTCGACCTCCAAGGCACCATCGACTGCGGCGACGCAGCCGAACAAGCCGCCAAAGACTTCGGCGGGATGAACTCCCTCGCTCCTCTCGCCTTGGTCCGTCCCGCCGGCGCCGCCGACGTTGCGAGCGTGGTGCGAGCGGCGGCGAATCTGAACCTGACGGCGGCGGCGCGCGGCAACGGCCACTCCATCAACGGCCAGGCCATGGCGGATCGAGGCCTGGTCCTGGACATGCCGGCCATGGAGGACCATTTCCAGGTGGTGAGGACGAGCAGCACCTCCGGCTACGCTGACGTGTCGGGAGGGGCATTATGGGAAGACGTGCTGAAACGGTGCGTTCGGGATTACGGCATGGCGCCGAGGTCGTGGACGGACTACCTCAGCTTAACGGTCGGCGGCACTTTATCCAACGCTGGCGTCAGCGGCCAGGCTTTCCGTTACGGACCCCAAACCTCGAACGTAACGGAGCTTGAAGTCGTTACGGGGAAGGGCGAGACTTTCGTGTGCTCGGAAGAGGAAAACTCGGAGCTCTTCTTTTCCGTTCTTGGCGGGCTAGGCCAGTTCGGCATCATCACCAGAGCTCGAGTACGCCTCCAGCAAGCCCCGGACATGGTGAGATGGATAAGGCTGGTGTACACCGAGTTCGACGCCTTCACTCGCGACGCCGAGTCGCTCGTCACTCGCCCCCACGACGACGGAGAGTCATTCGATTATGTGGAAGGCTTTGCCTTTATGAACAGCGATAACCCGGCGGATGGGCTCCCCTCGGTTCCTCTACACCCGGACCAGGTGTTCGACCCGACCCGGCTGCCTCAAGAAGCTGGATCAGTACTCTACTGTCTCGAGCTCGCTCGTCACTACCGGAGCTCTGACCACCTCTCAGCTGTTGACAAGGGCGTGAACAGGCTGCTGGAAGGCCTAGGATTCGTGGAGGGATTGGAGTTCCAAGTGGACCTCGGATACGAGGAGTTTCTATTGCGTGTGAAGCAGGCAGAGGAGCACGCAAAGGCCAGCGGCATTTGGGACGCTCCCCACCCTTGGCTGAACCTGTTCGTGTCAAAATCGGACATCGCCGATTTTGATCGGGTGGTGTTCAAGGAGATCCTTAAAGACGGCATTGGTGGCCCAATGCTCGTCTATCCTCTTCTGTGTAGCAAGTGGGATGCGCGGACGTCTGTTGTGCTACCGGAAGGCGAGATCTTCTACATTGTGGCGTTGTTGAGGTTCACTCCGGCATACCCGAAAGGCCCGTCGTGTGAGAGTCTAGTTGCTCAGAACCAGGAGATTGTGCAATATTGCAGGAAGAAAGGTTTGGACTTTAAGTTGTATCTGCCGCATTATCGATCGCAAGAGGAATGGAAGCGGCACTTCGGGAATCAATGGAGCAGGTTCGTCGAACGGAAGACTTGCTTCGATCCCATGGCAATTCTCGCTCCCGGGCAGAAAATTTTCCCGAGAAGTAATCCCAAACCCTAG

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0003674 GO:0003824 GO:0006725 GO:0006807 GO:0008150 GO:0008152 GO:0009056 GO:0009308 GO:0009690 GO:0009823 GO:0009987 GO:0010817 GO:0016491 GO:0016645 GO:0019139 GO:0034754 GO:0042445 GO:0042447 GO:0044237 GO:0046483 GO:0055114 GO:0065007 GO:0065008 GO:0071704 GO:1901564

KEGG Pathways

Metabolic & Signaling

ko00908 map00908

Pfam Domains

Protein Families

Cytokin-bind FAD_binding_4

Protein Analysis

521

Amino Acids

57.49

Weight (kDa)

5.01

Isoelectric Point (pI)

36.64

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
FAD_binding_4	PF01565	58 - 199	5.5e-25	FAD binding domain
Cytokin-bind	PF09265	231 - 414	1.1e-61	Cytokinin dehydrogenase 1, FAD and cytokinin binding

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0008557)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT5G21482
fragaria_vesca	FvH4_2g30990 FvH4_2g30990 FvH4_2g30990 FvH4_2g30990 FvH4_2g30990 FvH4_3g04610
malus_domestica	MD08G1071200.v1.1 MD15G1050100.v1.1
prunus_persica	Prupe.1G404300_v2.0.a1 Prupe.1G404300_v2.0.a1 Prupe.1G404300_v2.0.a1
pyrus_communis	pycom08g05900 pycom15g04860
rosa_chinensis	RchiOBHm_Chr6g0311781
rosa_laevigata	RLG00000010374
rosa_multiflora	Rmu_sc0002888.1_g000042
rosa_roxburghii	Rroxscaffold_7G00157310
rosa_samantha	Rh6AG507000 Rh6BG517900 Rh6CG523500 Rh6DG509300
rosa_wichuraiana	Rw6G044110

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AatII	GACGTC	2 cut(s)	69, 1262
Acc36I	ACCTGC	1 cut(s)	1464
AccB1I	GGYRCC	3 cut(s)	104, 197, 442
AccB7I	CCANNNNNTGG	1 cut(s)	292
AccBSI	CCGCTC	1 cut(s)	227
AccII	CGCG	5 cut(s)	84, 259, 261, 747, 1255
AclWI	GGATC	4 cut(s)	306, 919, 1183, 1496
AcoI	YGGCCR	5 cut(s)	56, 268, 283, 324, 511
AcsI	RAATTY	1 cut(s)	1533
AcyI	GRCGYC	8 cut(s)	45, 66, 198, 443, 504, 735, 750, 1259
AfaI	GTAC	3 cut(s)	675, 722, 918
AflIII	ACRYGT	1 cut(s)	381
AgsI	TTSAA	2 cut(s)	560, 1183
AhdI	GACNNNNNGTC	2 cut(s)	754, 787
AjiI	CACGTC	2 cut(s)	382, 409
AjnI	CCWGG	8 cut(s)	12, 285, 306, 312, 342, 513, 873, 1365
AjuI	GAANNNNNNNTTGG	2 cut(s)	1382, 1414
AloI	GAACNNNNNNTCC	2 cut(s)	863, 895
AluBI	AGCT	9 cut(s)	8, 471, 556, 616, 668, 908, 934, 954, 971
AluI	AGCT	9 cut(s)	8, 471, 556, 616, 668, 908, 934, 954, 971
Alw21I	GWGCWC	6 cut(s)	596, 618, 670, 936, 956, 1089
Alw26I	GTCTC	3 cut(s)	76, 575, 932
AlwI	GGATC	4 cut(s)	306, 919, 1183, 1496
Ama87I	CYCGRG	4 cut(s)	669, 929, 1523, 1540
ApaI	GGGCCC	1 cut(s)	91
ApeKI	GCWGC	4 cut(s)	125, 360, 895, 994
ApoI	RAATTY	1 cut(s)	1533
ArsI	GACNNNNNNTTYG	4 cut(s)	863, 895, 1322, 1354
Asp700I	GAANNNNTTC	2 cut(s)	1136, 1535
AspA2I	CCTAGG	1 cut(s)	1006
AspLEI	GCGC	5 cut(s)	200, 259, 261, 445, 1255
AsuC2I	CCSGG	7 cut(s)	55, 692, 836, 869, 892, 1524, 1525
AsuHPI	GGTGA	3 cut(s)	361, 652, 712
AvaI	CYCGRG	4 cut(s)	669, 929, 1523, 1540
AvaII	GGWCC	6 cut(s)	184, 310, 334, 530, 871, 1039
AvrII	CCTAGG	1 cut(s)	1006
BaeGI	GKGCMC	1 cut(s)	91
BanI	GGYRCC	3 cut(s)	104, 197, 442
BanII	GRGCYC	6 cut(s)	91, 618, 670, 849, 936, 956
BbsI	GAAGAC	2 cut(s)	411, 1496
Bbv12I	GWGCWC	6 cut(s)	596, 618, 670, 936, 956, 1089
BbvCI	CCTCAGC	1 cut(s)	467
BbvI	GCAGC	4 cut(s)	137, 372, 882, 981
BccI	CCATC	4 cut(s)	116, 284, 699, 836
BceAI	ACGGC	5 cut(s)	264, 283, 298, 451, 1216
BcgI	CGANNNNNNTGC	6 cut(s)	202, 236, 877, 911, 1462, 1496
BciT130I	CCWGG	8 cut(s)	14, 287, 308, 314, 344, 515, 875, 1367
BciVI	GTATCC	1 cut(s)	1040
BcnI	CCSGG	7 cut(s)	55, 692, 836, 869, 892, 1524, 1525
BcoDI	GTCTC	3 cut(s)	76, 575, 932
BfaI	CTAG	4 cut(s)	641, 1007, 1352, 1561
BfoI	RGCGCY	2 cut(s)	201, 446
BfuAI	ACCTGC	1 cut(s)	1464
BfuI	GTATCC	1 cut(s)	1040
BglI	GCCNNNNNGGC	1 cut(s)	651
BglII	AGATCT	1 cut(s)	1284
BlnI	CCTAGG	1 cut(s)	1006
BmcAI	AGTACT	1 cut(s)	918
Bme18I	GGWCC	6 cut(s)	184, 310, 334, 530, 871, 1039
BmeRI	GACNNNNNGTC	2 cut(s)	754, 787
BmeT110I	CYCGRG	4 cut(s)	669, 929, 1523, 1540
BmgBI	CACGTC	2 cut(s)	382, 409
BmiI	GGNNCC	8 cut(s)	88, 89, 106, 199, 444, 532, 848, 858
BmsI	GCATC	2 cut(s)	663, 1240
BpiI	GAAGAC	2 cut(s)	411, 1496
BplI	GAGNNNNNCTC	6 cut(s)	593, 625, 663, 695, 1300, 1332
BpmI	CTGGAG	2 cut(s)	35, 665
Bpu10I	CCTNAGC	1 cut(s)	467
BpuEI	CTTGAG	1 cut(s)	885
BpuMI	CCSGG	7 cut(s)	55, 692, 836, 869, 892, 1524, 1525
Bsa29I	ATCGAT	1 cut(s)	1429
BsaBI	GATNNNNATC	1 cut(s)	1167
BsaHI	GRCGYC	8 cut(s)	45, 66, 198, 443, 504, 735, 750, 1259
BsaWI	WCCGGW	2 cut(s)	948, 1273
BsaXI	ACNNNNNCTCC	4 cut(s)	56, 86, 257, 287
Bse118I	RCCGGY	2 cut(s)	194, 322
Bse1I	ACTGG	1 cut(s)	646
Bse8I	GATNNNNATC	1 cut(s)	1167
BseBI	CCWGG	8 cut(s)	14, 287, 308, 314, 344, 515, 875, 1367
BseCI	ATCGAT	1 cut(s)	1429
BseGI	GGATG	3 cut(s)	162, 847, 1255
BseJI	GATNNNNATC	1 cut(s)	1167
BseMII	CTCAG	3 cut(s)	481, 981, 1373
BseNI	ACTGG	1 cut(s)	646
BsePI	GCGCGC	1 cut(s)	257
BseRI	GAGGAG	3 cut(s)	162, 1067, 1097
BseSI	GKGCMC	1 cut(s)	91
BseXI	GCAGC	4 cut(s)	137, 372, 882, 981
Bsh1236I	CGCG	5 cut(s)	84, 259, 261, 747, 1255
Bsh1285I	CGRYCG	2 cut(s)	480, 1433
BshNI	GGYRCC	3 cut(s)	104, 197, 442
BshVI	ATCGAT	1 cut(s)	1429
BsiEI	CGRYCG	2 cut(s)	480, 1433
BsiHKAI	GWGCWC	6 cut(s)	596, 618, 670, 936, 956, 1089
BsiHKCI	CYCGRG	4 cut(s)	669, 929, 1523, 1540
BslFI	GGGAC	2 cut(s)	174, 1124
BsmAI	GTCTC	3 cut(s)	76, 575, 932
BsmBI	CGTCTC	1 cut(s)	76
BsmFI	GGGAC	2 cut(s)	174, 1124
BsoBI	CYCGRG	4 cut(s)	669, 929, 1523, 1540
Bsp120I	GGGCCC	1 cut(s)	87
Bsp1286I	GDGCHC	8 cut(s)	91, 596, 618, 670, 849, 936, 956, 1089
Bsp1407I	TGTACA	1 cut(s)	720
Bsp143I	GATC	7 cut(s)	298, 911, 1168, 1188, 1284, 1430, 1501
Bsp19I	CCATGG	3 cut(s)	291, 327, 1506
Bsp68I	TCGCGA	1 cut(s)	747
BspCNI	CTCAG	3 cut(s)	480, 980, 1372
BspDI	ATCGAT	1 cut(s)	1429
BspFNI	CGCG	5 cut(s)	84, 259, 261, 747, 1255
BspLI	GGNNCC	8 cut(s)	88, 89, 106, 199, 444, 532, 848, 858
BspMI	ACCTGC	1 cut(s)	1464
BspPI	GGATC	4 cut(s)	306, 919, 1183, 1496
BspQI	GCTCTTC	1 cut(s)	623
BspT107I	GGYRCC	3 cut(s)	104, 197, 442
BsrBI	CCGCTC	1 cut(s)	227
BsrFI	RCCGGY	2 cut(s)	194, 322
BsrGI	TGTACA	1 cut(s)	720
BsrI	ACTGG	1 cut(s)	646
BssAI	RCCGGY	2 cut(s)	194, 322
BssHII	GCGCGC	1 cut(s)	257
BssMI	GATC	7 cut(s)	298, 911, 1168, 1188, 1284, 1430, 1501
BssNI	GRCGYC	8 cut(s)	45, 66, 198, 443, 504, 735, 750, 1259
BssT1I	CCWWGG	7 cut(s)	100, 180, 291, 327, 1006, 1124, 1506
Bst2UI	CCWGG	8 cut(s)	14, 287, 308, 314, 344, 515, 875, 1367
Bst4CI	ACNGT	3 cut(s)	420, 478, 926
Bst6I	CTCTTC	3 cut(s)	594, 623, 1236
BstACI	GRCGYC	8 cut(s)	45, 66, 198, 443, 504, 735, 750, 1259
BstAUI	TGTACA	1 cut(s)	720
BstDEI	CTNAG	3 cut(s)	467, 967, 1359
BstDSI	CCRYGG	4 cut(s)	82, 291, 327, 1506
BstF5I	GGATG	3 cut(s)	162, 847, 1255
BstFNI	CGCG	5 cut(s)	84, 259, 261, 747, 1255
BstH2I	RGCGCY	2 cut(s)	201, 446
BstHHI	GCGC	5 cut(s)	200, 259, 261, 445, 1255
BstKTI	GATC	7 cut(s)	301, 914, 1171, 1191, 1287, 1433, 1504
BstMAI	GTCTC	3 cut(s)	76, 575, 932
BstMBI	GATC	7 cut(s)	298, 911, 1168, 1188, 1284, 1430, 1501
BstMCI	CGRYCG	2 cut(s)	480, 1433
BstNI	CCWGG	8 cut(s)	14, 287, 308, 314, 344, 515, 875, 1367
BstNSI	RCATGY	1 cut(s)	322
BstSLI	GKGCMC	1 cut(s)	91
BstUI	CGCG	5 cut(s)	84, 259, 261, 747, 1255
BstV1I	GCAGC	4 cut(s)	137, 372, 882, 981
BstV2I	GAAGAC	2 cut(s)	411, 1496
BstX2I	RGATCY	2 cut(s)	1188, 1284
BstYI	RGATCY	2 cut(s)	1188, 1284
Bsu15I	ATCGAT	1 cut(s)	1429
BsuI	GTATCC	1 cut(s)	1040
BsuTUI	ATCGAT	1 cut(s)	1429
BtgI	CCRYGG	4 cut(s)	82, 291, 327, 1506
BtgZI	GCGATG	1 cut(s)	1141
BtrI	CACGTC	2 cut(s)	382, 409
BtsCI	GGATG	3 cut(s)	162, 847, 1255
BtuMI	TCGCGA	1 cut(s)	747
BveI	ACCTGC	1 cut(s)	1464
CfoI	GCGC	5 cut(s)	200, 259, 261, 445, 1255
Cfr10I	RCCGGY	2 cut(s)	194, 322
Cfr42I	CCGCGG	1 cut(s)	85
Cfr9I	CCCGGG	1 cut(s)	1523
ClaI	ATCGAT	1 cut(s)	1429
CseI	GACGC	6 cut(s)	53, 131, 493, 743, 758, 1121
CsiI	ACCWGGT	1 cut(s)	873
Csp6I	GTAC	3 cut(s)	674, 721, 917
CviAII	CATG	6 cut(s)	292, 319, 328, 439, 697, 1507
CviQI	GTAC	3 cut(s)	674, 721, 917
DdeI	CTNAG	3 cut(s)	467, 967, 1359
DinI	GGCGCC	2 cut(s)	199, 444
DpnI	GATC	7 cut(s)	300, 913, 1170, 1190, 1286, 1432, 1503
DpnII	GATC	7 cut(s)	298, 911, 1168, 1188, 1284, 1430, 1501
DriI	GACNNNNNGTC	2 cut(s)	754, 787
EaeI	YGGCCR	5 cut(s)	56, 268, 283, 324, 511
Eam1104I	CTCTTC	3 cut(s)	594, 623, 1236
Eam1105I	GACNNNNNGTC	2 cut(s)	754, 787
EarI	CTCTTC	3 cut(s)	594, 623, 1236
EciI	GGCGGA	2 cut(s)	311, 854
Ecl136II	GAGCTC	4 cut(s)	616, 668, 934, 954
Eco130I	CCWWGG	7 cut(s)	100, 180, 291, 327, 1006, 1124, 1506
Eco147I	AGGCCT	2 cut(s)	306, 1005
Eco24I	GRGCYC	6 cut(s)	91, 618, 670, 849, 936, 956
Eco47I	GGWCC	6 cut(s)	184, 310, 334, 530, 871, 1039
Eco53kI	GAGCTC	4 cut(s)	616, 668, 934, 954
Eco88I	CYCGRG	4 cut(s)	669, 929, 1523, 1540
EcoICRI	GAGCTC	4 cut(s)	616, 668, 934, 954
EcoO109I	RGGNCCY	2 cut(s)	87, 88
EcoRII	CCWGG	8 cut(s)	12, 285, 306, 312, 342, 513, 873, 1365
EcoT14I	CCWWGG	7 cut(s)	100, 180, 291, 327, 1006, 1124, 1506
EcoT38I	GRGCYC	6 cut(s)	91, 618, 670, 849, 936, 956
EgeI	GGCGCC	2 cut(s)	199, 444
EheI	GGCGCC	2 cut(s)	199, 444
ErhI	CCWWGG	7 cut(s)	100, 180, 291, 327, 1006, 1124, 1506
Esp3I	CGTCTC	1 cut(s)	76
FaeI	CATG	6 cut(s)	295, 322, 331, 442, 700, 1510
FalI	AAGNNNNNCTT	4 cut(s)	1439, 1471, 1481, 1513
FaqI	GGGAC	2 cut(s)	174, 1124
FatI	CATG	6 cut(s)	291, 318, 327, 438, 696, 1506
FauI	CCCGC	4 cut(s)	77, 146, 199, 629
FokI	GGATG	3 cut(s)	169, 854, 1262
FriOI	GRGCYC	6 cut(s)	91, 618, 670, 849, 936, 956
FspBI	CTAG	4 cut(s)	641, 1007, 1352, 1561
GlaI	GCGC	5 cut(s)	199, 258, 260, 444, 1254
GsuI	CTGGAG	2 cut(s)	35, 665
HaeII	RGCGCY	2 cut(s)	201, 446
HgaI	GACGC	6 cut(s)	53, 131, 493, 743, 758, 1121
HhaI	GCGC	5 cut(s)	200, 259, 261, 445, 1255
Hin1I	GRCGYC	8 cut(s)	45, 66, 198, 443, 504, 735, 750, 1259
Hin1II	CATG	6 cut(s)	295, 322, 331, 442, 700, 1510
Hin6I	GCGC	5 cut(s)	198, 257, 259, 443, 1253
HinP1I	GCGC	5 cut(s)	198, 257, 259, 443, 1253
HincII	GTYRAC	1 cut(s)	976
HindII	GTYRAC	1 cut(s)	976
HinfI	GANTC	7 cut(s)	50, 235, 755, 788, 1011, 1348, 1462
HphI	GGTGA	3 cut(s)	361, 652, 712
Hpy166II	GTNNAC	7 cut(s)	456, 721, 723, 976, 988, 1039, 1314
Hpy188I	TCNGA	7 cut(s)	240, 598, 613, 958, 1046, 1153, 1362
Hpy188III	TCNNGA	7 cut(s)	387, 428, 746, 902, 929, 1459, 1540
Hpy8I	GTNNAC	7 cut(s)	456, 721, 723, 976, 988, 1039, 1314
HpyAV	CCTTC	5 cut(s)	568, 748, 800, 995, 1271
HpyCH4III	ACNGT	3 cut(s)	420, 478, 926
HpyCH4IV	ACGT	6 cut(s)	66, 207, 381, 408, 546, 1259
HpyCH4V	TGCA	2 cut(s)	1378, 1386
HpyF3I	CTNAG	3 cut(s)	467, 967, 1359
HpySE526I	ACGT	6 cut(s)	66, 207, 381, 408, 546, 1259
Hsp92I	GRCGYC	8 cut(s)	45, 66, 198, 443, 504, 735, 750, 1259
Hsp92II	CATG	6 cut(s)	295, 322, 331, 442, 700, 1510
HspAI	GCGC	5 cut(s)	198, 257, 259, 443, 1253
KasI	GGCGCC	2 cut(s)	197, 442
KroI	GCCGGC	2 cut(s)	194, 322
KroNI	GCCGGC	2 cut(s)	196, 324
KspI	CCGCGG	1 cut(s)	85
Kzo9I	GATC	7 cut(s)	298, 911, 1168, 1188, 1284, 1430, 1501
LguI	GCTCTTC	1 cut(s)	623
LmnI	GCTCC	9 cut(s)	175, 553, 613, 852, 951, 1084, 1120, 1470, 1525
Lsp1109I	GCAGC	4 cut(s)	137, 372, 882, 981
LweI	GCATC	2 cut(s)	663, 1240
MabI	ACCWGGT	1 cut(s)	873
MaeI	CTAG	4 cut(s)	641, 1007, 1352, 1561
MaeII	ACGT	6 cut(s)	66, 207, 381, 408, 546, 1259
MaeIII	GTNAC	5 cut(s)	524, 547, 565, 763, 941
MalI	GATC	7 cut(s)	300, 913, 1170, 1190, 1286, 1432, 1503
MbiI	CCGCTC	1 cut(s)	227
MboI	GATC	7 cut(s)	298, 911, 1168, 1188, 1284, 1430, 1501
MboII	GAAGA	7 cut(s)	416, 610, 611, 1223, 1279, 1402, 1501
MflI	RGATCY	2 cut(s)	1188, 1284
MhlI	GDGCHC	8 cut(s)	91, 596, 618, 670, 849, 936, 956, 1089
MluCI	AATT	2 cut(s)	1512, 1533
Mly113I	GGCGCC	2 cut(s)	198, 443
MlyI	GAGTC	3 cut(s)	764, 797, 1357
MmeI	TCCRAC	1 cut(s)	519
MreI	CGCCGGCG	1 cut(s)	194
MroNI	GCCGGC	2 cut(s)	194, 322
MroXI	GAANNNNTTC	2 cut(s)	1136, 1535
MseI	TTAA	3 cut(s)	473, 1194, 1407
MspA1I	CMGCKG	4 cut(s)	84, 510, 971, 1101
MvaI	CCWGG	8 cut(s)	14, 287, 308, 314, 344, 515, 875, 1367
MvnI	CGCG	5 cut(s)	84, 259, 261, 747, 1255
NaeI	GCCGGC	2 cut(s)	196, 324
NarI	GGCGCC	2 cut(s)	198, 443
NciI	CCSGG	7 cut(s)	55, 692, 836, 869, 892, 1524, 1525
NcoI	CCATGG	3 cut(s)	291, 327, 1506
NdeII	GATC	7 cut(s)	298, 911, 1168, 1188, 1284, 1430, 1501
NgoMIV	GCCGGC	2 cut(s)	194, 322
NlaIII	CATG	6 cut(s)	295, 322, 331, 442, 700, 1510
NlaIV	GGNNCC	8 cut(s)	88, 89, 106, 199, 444, 532, 848, 858
NmeAIII	GCCGAG	2 cut(s)	471, 778
NmuCI	GTSAC	2 cut(s)	763, 941
NruI	TCGCGA	1 cut(s)	747
NspI	RCATGY	1 cut(s)	322
PaeR7I	CTCGAG	2 cut(s)	669, 929
PauI	GCGCGC	1 cut(s)	257
PceI	AGGCCT	2 cut(s)	306, 1005
PciSI	GCTCTTC	1 cut(s)	623
PcsI	WCGNNNNNNNCGW	2 cut(s)	117, 213
PdiI	GCCGGC	2 cut(s)	196, 324
PdmI	GAANNNNTTC	2 cut(s)	1136, 1535
PfeI	GAWTC	4 cut(s)	50, 235, 1011, 1462
PflFI	GACNNNGTC	1 cut(s)	68
PflMI	CCANNNNNTGG	1 cut(s)	292
PfoI	TCCNGGA	1 cut(s)	312
Ple19I	CGATCG	1 cut(s)	1433
PleI	GAGTC	3 cut(s)	763, 796, 1356
PluTI	GGCGCC	2 cut(s)	201, 446
PpsI	GAGTC	3 cut(s)	763, 796, 1356
Psp124BI	GAGCTC	4 cut(s)	618, 670, 936, 956
Psp6I	CCWGG	8 cut(s)	12, 285, 306, 312, 342, 513, 873, 1365
PspGI	CCWGG	8 cut(s)	12, 285, 306, 312, 342, 513, 873, 1365
PspN4I	GGNNCC	8 cut(s)	88, 89, 106, 199, 444, 532, 848, 858
PspOMI	GGGCCC	1 cut(s)	87
PspXI	VCTCGAGB	1 cut(s)	669
PsrI	GAACNNNNNNTAC	2 cut(s)	713, 745
PsuI	RGATCY	2 cut(s)	1188, 1284
PsyI	GACNNNGTC	1 cut(s)	68
PteI	GCGCGC	1 cut(s)	257
PvuI	CGATCG	1 cut(s)	1433
PvuII	CAGCTG	1 cut(s)	971
RruI	TCGCGA	1 cut(s)	747
RsaI	GTAC	3 cut(s)	675, 722, 918
RsaNI	GTAC	3 cut(s)	674, 721, 917
SacI	GAGCTC	4 cut(s)	618, 670, 936, 956
SacII	CCGCGG	1 cut(s)	85
SapI	GCTCTTC	1 cut(s)	623
SaqAI	TTAA	3 cut(s)	473, 1194, 1407
Sau3AI	GATC	7 cut(s)	298, 911, 1168, 1188, 1284, 1430, 1501
ScaI	AGTACT	1 cut(s)	918
SchI	GAGTC	3 cut(s)	764, 797, 1357
SduI	GDGCHC	8 cut(s)	91, 596, 618, 670, 849, 936, 956, 1089
SexAI	ACCWGGT	1 cut(s)	873
SfaNI	GCATC	2 cut(s)	663, 1240
SfoI	GGCGCC	2 cut(s)	199, 444
Sfr274I	CTCGAG	2 cut(s)	669, 929
Sfr303I	CCGCGG	1 cut(s)	85
SgrAI	CRCCGGYG	1 cut(s)	194
SgrBI	CCGCGG	1 cut(s)	85
SinI	GGWCC	6 cut(s)	184, 310, 334, 530, 871, 1039
SlaI	CTCGAG	2 cut(s)	669, 929
SmaI	CCCGGG	1 cut(s)	1525
SmlI	CTYRAG	3 cut(s)	669, 900, 929
SmoI	CTYRAG	3 cut(s)	669, 900, 929
Sse9I	AATT	2 cut(s)	1512, 1533
SseBI	AGGCCT	2 cut(s)	306, 1005
SspDI	GGCGCC	2 cut(s)	197, 442
SspI	AATATT	1 cut(s)	1382
SspMI	CTAG	4 cut(s)	641, 1007, 1352, 1561
SstI	GAGCTC	4 cut(s)	618, 670, 936, 956
StuI	AGGCCT	2 cut(s)	306, 1005
StyI	CCWWGG	7 cut(s)	100, 180, 291, 327, 1006, 1124, 1506
TaaI	ACNGT	3 cut(s)	420, 478, 926
TaiI	ACGT	6 cut(s)	69, 210, 384, 411, 549, 1262
TasI	AATT	2 cut(s)	1512, 1533
TatI	WGTACW	2 cut(s)	720, 916
TfiI	GAWTC	4 cut(s)	50, 235, 1011, 1462
Tru1I	TTAA	3 cut(s)	473, 1194, 1407
Tru9I	TTAA	3 cut(s)	473, 1194, 1407
TseFI	GTSAC	2 cut(s)	763, 941
TseI	GCWGC	4 cut(s)	125, 360, 895, 994
Tsp45I	GTSAC	2 cut(s)	763, 941
TspDTI	ATGAA	3 cut(s)	51, 173, 836
TspGWI	ACGGA	8 cut(s)	176, 473, 512, 543, 566, 616, 798, 1501
TspMI	CCCGGG	1 cut(s)	1523
Tth111I	GACNNNGTC	1 cut(s)	68
Van91I	CCANNNNNTGG	1 cut(s)	292
VpaK11BI	GGWCC	6 cut(s)	184, 310, 334, 530, 871, 1039
XapI	RAATTY	1 cut(s)	1533
XceI	RCATGY	1 cut(s)	322
XhoI	CTCGAG	2 cut(s)	669, 929
XmaI	CCCGGG	1 cut(s)	1523
XmaJI	CCTAGG	1 cut(s)	1006
XmnI	GAANNNNTTC	2 cut(s)	1136, 1535
XspI	CTAG	4 cut(s)	641, 1007, 1352, 1561
ZraI	GACGTC	2 cut(s)	67, 1260
ZrmI	AGTACT	1 cut(s)	918

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.