Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr08

Orthogroup ID

OG0008557

Physical Location & Seq

Genomic Coordinates Forward (+)

5651835 .. 5656146

Gene Structure 4312 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

MD08G1071200.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 1542 bp

ATGATAGCTTACCTGGAATGCTTCGTCTCCGAATCGAGGCACGACGACGGCGTTTCGAGCCTCTCCAAGGCGCTGGGCCTCCAGGGCACCGTCGCCGACTTTGGCGGCGAAGCCGGCAGAGACTTCGGGGGAATGAAGTCGTTCCCGCCGCTGGCTTTCATCAGGCCGGCCGGCGCCGACGACGTGGCGAGGGTGGTGAAGGAGGCGGCGAGGTCCTCGAATCTGACGGTGGCGGCGCGGGGGAACGGCCACTCGATCAACGGCCAGGCGATGGCGGATCGAGGCCTCGTCCTGGACATGCGTTCGTTGGGGGAGCATCTCCGGGTTGTGAGGGCCAGCGACGGTTCCGTCTACGCTGACGTGTCGGGAGGGGCATTATGGGAAGACGTGCTGAAACGGTGCGTTTCCGAGCACGGGATGGCGCCGCGATCGTGGACTGATTACTTGAGCTTGACGGTGGGCGGGACGTTATCCAACGCGGGCGTCAGCGGTCAGGCTTTCCGTTACGGTCCACAAACGTCGAACGTTACGGAGTTAGAAGTCGTTACGGGGAAGGGAGAAATTTTTAACTGCTCCGAAAAGGAAAACTCGGAGCTGTTTTTCGGCGCACTTGGCGGACTCGGTCAGTTCGGTATCATCACCAAAGCTAAGGTTCTTCTGCAACCCGCCCCAGACATGGTAAGATGGATAAGGCTGGTGTACACCGAGTTTGACGACTTCACTCGGGACGCCGAGTTGCTGGTGACTAGGCAGGACGACGGCGGCGACTCGTTCGACTACGTGGAAGGCTTCGCATTCGTCAACAGCGACAACCCGGCGGACGGGCGTCCCTCGGTGCCGCTGGATCCGCAGGAGACATTCGACCCGGCCCACCTCCCCCGAACCTCCGGGCCAATCCTCTACTGCCTCGAACTCGCTCATCACTACCGCAACACCGACCGCCCCTCATCCGTCGATACGGGCGTGAACAGATTGCTTGGAGGCCTCGGATTCGTTGAGGAGTTGAAGTTCCAAGTGGACCTCAGCTACGTCGAGTTTTTATTGCGTGTGAAGCGCGCAGAGGAACACGCCAAGGCCAATGGAATTTGGGACGCTCCTCACCCTTGGTTGAACATGTTCGTGTCGAAATCAGATATCGCTGATTTCGATCGAACGGTGTTCAAGAAGATCCTCAAGGATGGGATCGGAGGGCCCATGCTTGTCTACCCTCTCCGGCGAAGCAAGTGGGATACTCGTACATCTGTGGTGCTACCGGAAAGCGAAATCTTCTACATAGTGGCATTGCTACGCTTCACTCCACCATACCCAAAAGGCCCCTCCGCCGAGAATCTGGTGGCCCAAAACCAAGAAATAATACAATACTGCACCAGAAAAGGGTTCGATTTCAAGCTTTATCTGCCTCACTATCGATCCCAGGAGGATTGGAAGCGACATTTCGGAAGTGATCAATGGTCGAGATTCGTGGAGCGGAAGAAGTGCTTCGATCCAATGGCCATCCTTGCTCCTGGACAGAAAATCTTCTCGAGGGTTCCTCAACCCTAG

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0003674 GO:0003824 GO:0006725 GO:0006807 GO:0008150 GO:0008152 GO:0009056 GO:0009308 GO:0009690 GO:0009823 GO:0009987 GO:0010817 GO:0016491 GO:0016645 GO:0019139 GO:0034754 GO:0042445 GO:0042447 GO:0044237 GO:0046483 GO:0055114 GO:0065007 GO:0065008 GO:0071704 GO:1901564

KEGG Pathways

Metabolic & Signaling

ko00908 map00908

Pfam Domains

Protein Families

Cytokin-bind FAD_binding_4

Protein Analysis

514

Amino Acids

56.91

Weight (kDa)

5.84

Isoelectric Point (pI)

40.15

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
FAD_binding_4	PF01565	50 - 192	6.4e-25	FAD binding domain
Cytokin-bind	PF09265	224 - 507	1.7e-110	Cytokinin dehydrogenase 1, FAD and cytokinin binding

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

Style Settings

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

iTOL Interactive Tree Of Life · Most popular EvolView Flexible annotations MEGA Desktop phylogenetics suite FigTree Desktop tree editor

Orthologous Genes (Group: OG0008557)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT5G21482
fragaria_vesca	FvH4_2g30990 FvH4_2g30990 FvH4_2g30990 FvH4_2g30990 FvH4_2g30990 FvH4_3g04610
malus_domestica	MD08G1071200.v1.1 MD15G1050100.v1.1
prunus_persica	Prupe.1G404300_v2.0.a1 Prupe.1G404300_v2.0.a1 Prupe.1G404300_v2.0.a1
pyrus_communis	pycom08g05900 pycom15g04860
rosa_chinensis	RchiOBHm_Chr6g0311781
rosa_laevigata	RLG00000010374
rosa_multiflora	Rmu_sc0002888.1_g000042
rosa_roxburghii	Rroxscaffold_7G00157310
rosa_samantha	Rh6AG507000 Rh6BG517900 Rh6CG523500 Rh6DG509300
rosa_wichuraiana	Rw6G044110

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AasI	GACNNNNNNGTC	1 cut(s)	347
AccB1I	GGYRCC	4 cut(s)	86, 173, 421, 835
AccB7I	CCANNNNNTGG	1 cut(s)	271
AccBSI	CCGCTC	1 cut(s)	1468
AccI	GTMKAC	2 cut(s)	351, 1203
AccII	CGCG	4 cut(s)	238, 427, 479, 1056
AclI	AACGTT	1 cut(s)	525
AclWI	GGATC	7 cut(s)	285, 839, 852, 1162, 1190, 1404, 1478
AcoI	YGGCCR	4 cut(s)	168, 247, 262, 1491
AcsI	RAATTY	2 cut(s)	561, 1083
AcyI	GRCGYC	5 cut(s)	174, 422, 483, 729, 826
AfaI	GTAC	2 cut(s)	701, 1237
AfiI	CCNNNNNNNGG	9 cut(s)	36, 67, 151, 271, 292, 414, 676, 821, 1374
AflIII	ACRYGT	2 cut(s)	360, 1113
AgsI	TTSAA	4 cut(s)	1006, 1111, 1162, 1387
AjiI	CACGTC	3 cut(s)	184, 361, 388
AjnI	CCWGG	6 cut(s)	12, 81, 264, 291, 1413, 1504
AloI	GAACNNNNNNTCC	2 cut(s)	992, 1024
AluBI	AGCT	6 cut(s)	8, 450, 595, 647, 1026, 1390
AluI	AGCT	6 cut(s)	8, 450, 595, 647, 1026, 1390
Alw21I	GWGCWC	1 cut(s)	414
Alw26I	GTCTC	3 cut(s)	31, 114, 848
AlwI	GGATC	7 cut(s)	285, 839, 852, 1162, 1190, 1404, 1478
Ama87I	CYCGRG	2 cut(s)	723, 1522
ApaI	GGGCCC	1 cut(s)	1194
ApoI	RAATTY	2 cut(s)	561, 1083
Asp700I	GAANNNNTTC	4 cut(s)	140, 1115, 1478, 1517
AspLEI	GCGC	7 cut(s)	73, 176, 238, 424, 608, 1056, 1058
AsuC2I	CCSGG	4 cut(s)	323, 815, 866, 889
AsuHPI	GGTGA	4 cut(s)	208, 631, 754, 1091
AvaI	CYCGRG	2 cut(s)	723, 1522
AvaII	GGWCC	3 cut(s)	213, 509, 1018
BaeGI	GKGCMC	2 cut(s)	89, 1194
BaeI	ACNNNNGTAYC	2 cut(s)	948, 981
BalI	TGGCCA	1 cut(s)	1493
BamHI	GGATCC	1 cut(s)	844
BanI	GGYRCC	4 cut(s)	86, 173, 421, 835
BanII	GRGCYC	1 cut(s)	1194
BbsI	GAAGAC	1 cut(s)	390
Bbv12I	GWGCWC	1 cut(s)	414
BbvCI	CCTCAGC	1 cut(s)	1022
BccI	CCATC	5 cut(s)	265, 412, 678, 1172, 1502
BceAI	ACGGC	4 cut(s)	64, 262, 277, 775
BcgI	CGANNNNNNTGC	2 cut(s)	106, 140
BciT130I	CCWGG	6 cut(s)	14, 83, 266, 293, 1415, 1506
BciVI	GTATCC	1 cut(s)	1222
BclI	TGATCA	1 cut(s)	1444
BcnI	CCSGG	4 cut(s)	323, 815, 866, 889
BcoDI	GTCTC	3 cut(s)	31, 114, 848
BfaI	CTAG	2 cut(s)	747, 1540
BfoI	RGCGCY	3 cut(s)	74, 177, 425
BfuI	GTATCC	1 cut(s)	1222
BglI	GCCNNNNNGGC	1 cut(s)	84
BisI	GCNGC	7 cut(s)	106, 149, 207, 234, 425, 763, 839
BlsI	GCNGC	7 cut(s)	107, 150, 208, 235, 426, 764, 840
Bme18I	GGWCC	3 cut(s)	213, 509, 1018
BmeT110I	CYCGRG	2 cut(s)	723, 1522
BmgBI	CACGTC	3 cut(s)	184, 361, 388
BmiI	GGNNCC	9 cut(s)	88, 175, 346, 423, 837, 846, 1192, 1315, 1530
BmsI	GCATC	1 cut(s)	325
BoxI	GACNNNNGTC	1 cut(s)	825
BpiI	GAAGAC	1 cut(s)	390
BplI	GAGNNNNNCTC	1 cut(s)	1516
BpmI	CTGGAG	1 cut(s)	65
Bpu10I	CCTNAGC	2 cut(s)	648, 1022
BpuEI	CTTGAG	2 cut(s)	466, 1157
BpuMI	CCSGG	4 cut(s)	323, 815, 866, 889
Bsa29I	ATCGAT	1 cut(s)	1408
BsaAI	YACGTR	1 cut(s)	781
BsaBI	GATNNNNATC	1 cut(s)	1146
BsaHI	GRCGYC	5 cut(s)	174, 422, 483, 729, 826
BsaJI	CCNNGG	7 cut(s)	66, 82, 831, 985, 1071, 1103, 1413
BsaWI	WCCGGW	1 cut(s)	1252
BsaXI	ACNNNNNCTCC	2 cut(s)	992, 1022
Bsc4I	CCNNNNNNNGG	9 cut(s)	36, 67, 151, 271, 292, 414, 676, 821, 1374
Bse118I	RCCGGY	3 cut(s)	113, 166, 170
Bse3DI	GCAATG	1 cut(s)	1280
Bse8I	GATNNNNATC	1 cut(s)	1146
BseBI	CCWGG	6 cut(s)	14, 83, 266, 293, 1415, 1506
BseCI	ATCGAT	1 cut(s)	1408
BseDI	CCNNGG	7 cut(s)	66, 82, 831, 985, 1071, 1103, 1413
BseGI	GGATG	4 cut(s)	423, 947, 1183, 1494
BseJI	GATNNNNATC	1 cut(s)	1146
BseLI	CCNNNNNNNGG	9 cut(s)	36, 67, 151, 271, 292, 414, 676, 821, 1374
BseMI	GCAATG	1 cut(s)	1280
BseMII	CTCAG	1 cut(s)	1036
BsePI	GCGCGC	1 cut(s)	1054
BseRI	GAGGAG	2 cut(s)	1013, 1086
BseSI	GKGCMC	2 cut(s)	89, 1194
BseX3I	CGGCCG	1 cut(s)	168
BseYI	CCCAGC	1 cut(s)	73
BsgI	GTGCAG	1 cut(s)	1348
Bsh1236I	CGCG	4 cut(s)	238, 427, 479, 1056
Bsh1285I	CGRYCG	4 cut(s)	171, 431, 940, 1150
BshNI	GGYRCC	4 cut(s)	86, 173, 421, 835
BshVI	ATCGAT	1 cut(s)	1408
BsiEI	CGRYCG	4 cut(s)	171, 431, 940, 1150
BsiHKAI	GWGCWC	1 cut(s)	414
BsiHKCI	CYCGRG	2 cut(s)	723, 1522
BsiSI	CCGG	9 cut(s)	114, 167, 171, 322, 815, 866, 888, 1213, 1253
BslFI	GGGAC	4 cut(s)	478, 740, 813, 1103
BslI	CCNNNNNNNGG	9 cut(s)	36, 67, 151, 271, 292, 414, 676, 821, 1374
BsmAI	GTCTC	3 cut(s)	31, 114, 848
BsmBI	CGTCTC	1 cut(s)	31
BsmFI	GGGAC	4 cut(s)	478, 740, 813, 1103
BsmI	GAATGC	2 cut(s)	23, 794
BsoBI	CYCGRG	2 cut(s)	723, 1522
Bsp120I	GGGCCC	1 cut(s)	1190
Bsp1286I	GDGCHC	3 cut(s)	89, 414, 1194
Bsp1407I	TGTACA	1 cut(s)	699
BspCNI	CTCAG	1 cut(s)	1035
BspDI	ATCGAT	1 cut(s)	1408
BspFNI	CGCG	4 cut(s)	238, 427, 479, 1056
BspLI	GGNNCC	9 cut(s)	88, 175, 346, 423, 837, 846, 1192, 1315, 1530
BspPI	GGATC	7 cut(s)	285, 839, 852, 1162, 1190, 1404, 1478
BspT107I	GGYRCC	4 cut(s)	86, 173, 421, 835
BsrBI	CCGCTC	1 cut(s)	1468
BsrDI	GCAATG	1 cut(s)	1280
BsrFI	RCCGGY	3 cut(s)	113, 166, 170
BsrGI	TGTACA	1 cut(s)	699
BssAI	RCCGGY	3 cut(s)	113, 166, 170
BssECI	CCNNGG	7 cut(s)	66, 82, 831, 985, 1071, 1103, 1413
BssHII	GCGCGC	1 cut(s)	1054
BssNI	GRCGYC	5 cut(s)	174, 422, 483, 729, 826
BssT1I	CCWWGG	3 cut(s)	66, 1071, 1103
Bst2UI	CCWGG	6 cut(s)	14, 83, 266, 293, 1415, 1506
Bst4CI	ACNGT	7 cut(s)	91, 229, 344, 399, 457, 509, 1156
BstACI	GRCGYC	5 cut(s)	174, 422, 483, 729, 826
BstAUI	TGTACA	1 cut(s)	699
BstBAI	YACGTR	1 cut(s)	781
BstC8I	GCNNGC	7 cut(s)	115, 153, 168, 172, 337, 481, 1056
BstDEI	CTNAG	2 cut(s)	648, 1022
BstENI	CCTNNNNNAGG	1 cut(s)	65
BstF5I	GGATG	4 cut(s)	423, 947, 1183, 1494
BstFNI	CGCG	4 cut(s)	238, 427, 479, 1056
BstH2I	RGCGCY	3 cut(s)	74, 177, 425
BstHHI	GCGC	7 cut(s)	73, 176, 238, 424, 608, 1056, 1058
BstMAI	GTCTC	3 cut(s)	31, 114, 848
BstMCI	CGRYCG	4 cut(s)	171, 431, 940, 1150
BstMWI	GCNNNNNNNGC	8 cut(s)	57, 84, 114, 612, 847, 1051, 1396, 1499
BstNI	CCWGG	6 cut(s)	14, 83, 266, 293, 1415, 1506
BstNSI	RCATGY	2 cut(s)	301, 1117
BstPAI	GACNNNNGTC	1 cut(s)	825
BstSLI	GKGCMC	2 cut(s)	89, 1194
BstUI	CGCG	4 cut(s)	238, 427, 479, 1056
BstV2I	GAAGAC	1 cut(s)	390
BstX2I	RGATCY	2 cut(s)	844, 1167
BstXI	CCANNNNNNTGG	1 cut(s)	73
BstYI	RGATCY	2 cut(s)	844, 1167
BstZI	CGGCCG	1 cut(s)	168
Bsu15I	ATCGAT	1 cut(s)	1408
BsuI	GTATCC	1 cut(s)	1222
BsuTUI	ATCGAT	1 cut(s)	1408
BtgZI	GCGATG	1 cut(s)	284
BtrI	CACGTC	3 cut(s)	184, 361, 388
BtsCI	GGATG	4 cut(s)	423, 947, 1183, 1494
Cac8I	GCNNGC	7 cut(s)	115, 153, 168, 172, 337, 481, 1056
CfoI	GCGC	7 cut(s)	73, 176, 238, 424, 608, 1056, 1058
Cfr10I	RCCGGY	3 cut(s)	113, 166, 170
ClaI	ATCGAT	1 cut(s)	1408
CseI	GACGC	4 cut(s)	472, 737, 815, 1100
Csp6I	GTAC	2 cut(s)	700, 1236
CviAII	CATG	4 cut(s)	298, 676, 1114, 1195
CviQI	GTAC	2 cut(s)	700, 1236
DdeI	CTNAG	2 cut(s)	648, 1022
DinI	GGCGCC	2 cut(s)	175, 423
DrdI	GACNNNNNNGTC	1 cut(s)	347
DseDI	GACNNNNNNGTC	1 cut(s)	347
EaeI	YGGCCR	4 cut(s)	168, 247, 262, 1491
EagI	CGGCCG	1 cut(s)	168
EciI	GGCGGA	4 cut(s)	290, 630, 833, 1309
EclXI	CGGCCG	1 cut(s)	168
Eco130I	CCWWGG	3 cut(s)	66, 1071, 1103
Eco147I	AGGCCT	2 cut(s)	285, 984
Eco24I	GRGCYC	1 cut(s)	1194
Eco32I	GATATC	1 cut(s)	1135
Eco47I	GGWCC	3 cut(s)	213, 509, 1018
Eco52I	CGGCCG	1 cut(s)	168
Eco88I	CYCGRG	2 cut(s)	723, 1522
EcoNI	CCTNNNNNAGG	1 cut(s)	65
EcoO109I	RGGNCCY	3 cut(s)	213, 1190, 1313
EcoRII	CCWGG	6 cut(s)	12, 81, 264, 291, 1413, 1504
EcoRV	GATATC	1 cut(s)	1135
EcoT14I	CCWWGG	3 cut(s)	66, 1071, 1103
EcoT38I	GRGCYC	1 cut(s)	1194
EgeI	GGCGCC	2 cut(s)	175, 423
EheI	GGCGCC	2 cut(s)	175, 423
ErhI	CCWWGG	3 cut(s)	66, 1071, 1103
Esp3I	CGTCTC	1 cut(s)	31
FaeI	CATG	4 cut(s)	301, 679, 1117, 1198
FaiI	YATR	7 cut(s)	299, 379, 677, 1115, 1196, 1274, 1303
FalI	AAGNNNNNCTT	2 cut(s)	1463, 1495
FaqI	GGGAC	4 cut(s)	478, 740, 813, 1103
FatI	CATG	4 cut(s)	297, 675, 1113, 1194
FauI	CCCGC	5 cut(s)	153, 231, 455, 472, 673
FbaI	TGATCA	1 cut(s)	1444
FblI	GTMKAC	2 cut(s)	351, 1203
Fnu4HI	GCNGC	7 cut(s)	106, 149, 207, 234, 425, 763, 839
FokI	GGATG	4 cut(s)	430, 934, 1190, 1481
FriOI	GRGCYC	1 cut(s)	1194
FseI	GGCCGGCC	1 cut(s)	170
Fsp4HI	GCNGC	7 cut(s)	106, 149, 207, 234, 425, 763, 839
FspBI	CTAG	2 cut(s)	747, 1540
GlaI	GCGC	7 cut(s)	72, 175, 237, 423, 607, 1055, 1057
GluI	GCNGC	7 cut(s)	106, 149, 207, 234, 425, 763, 839
GsaI	CCCAGC	1 cut(s)	77
GsuI	CTGGAG	1 cut(s)	65
HaeII	RGCGCY	3 cut(s)	74, 177, 425
HapII	CCGG	9 cut(s)	114, 167, 171, 322, 815, 866, 888, 1213, 1253
HgaI	GACGC	4 cut(s)	472, 737, 815, 1100
HhaI	GCGC	7 cut(s)	73, 176, 238, 424, 608, 1056, 1058
Hin1I	GRCGYC	5 cut(s)	174, 422, 483, 729, 826
Hin1II	CATG	4 cut(s)	301, 679, 1117, 1198
Hin6I	GCGC	7 cut(s)	71, 174, 236, 422, 606, 1054, 1056
HinP1I	GCGC	7 cut(s)	71, 174, 236, 422, 606, 1054, 1056
HincII	GTYRAC	1 cut(s)	802
HindII	GTYRAC	1 cut(s)	802
HindIII	AAGCTT	1 cut(s)	1388
HinfI	GANTC	7 cut(s)	32, 220, 618, 767, 990, 1327, 1458
HpaII	CCGG	9 cut(s)	114, 167, 171, 322, 815, 866, 888, 1213, 1253
HphI	GGTGA	4 cut(s)	208, 631, 754, 1091
Hpy166II	GTNNAC	9 cut(s)	352, 435, 512, 700, 702, 802, 967, 1018, 1204
Hpy188I	TCNGA	9 cut(s)	31, 225, 409, 577, 592, 989, 1132, 1187, 1439
Hpy188III	TCNNGA	5 cut(s)	366, 725, 1162, 1455, 1522
Hpy8I	GTNNAC	9 cut(s)	352, 435, 512, 700, 702, 802, 967, 1018, 1204
HpyAV	CCTTC	3 cut(s)	193, 547, 779
HpyCH4III	ACNGT	7 cut(s)	91, 229, 344, 399, 457, 509, 1156
HpyCH4IV	ACGT	8 cut(s)	183, 360, 387, 467, 518, 525, 780, 1029
HpyCH4V	TGCA	2 cut(s)	661, 1365
HpyF10VI	GCNNNNNNNGC	8 cut(s)	57, 84, 114, 612, 847, 1051, 1396, 1499
HpyF3I	CTNAG	2 cut(s)	648, 1022
HpySE526I	ACGT	8 cut(s)	183, 360, 387, 467, 518, 525, 780, 1029
Hsp92I	GRCGYC	5 cut(s)	174, 422, 483, 729, 826
Hsp92II	CATG	4 cut(s)	301, 679, 1117, 1198
HspAI	GCGC	7 cut(s)	71, 174, 236, 422, 606, 1054, 1056
KasI	GGCGCC	2 cut(s)	173, 421
KroI	GCCGGC	3 cut(s)	113, 166, 170
KroNI	GCCGGC	3 cut(s)	115, 168, 172
Ksp22I	TGATCA	1 cut(s)	1444
LmnI	GCTCC	6 cut(s)	313, 578, 592, 1099, 1465, 1507
LweI	GCATC	1 cut(s)	325
MaeI	CTAG	2 cut(s)	747, 1540
MaeII	ACGT	8 cut(s)	183, 360, 387, 467, 518, 525, 780, 1029
MaeIII	GTNAC	4 cut(s)	503, 526, 544, 742
MbiI	CCGCTC	1 cut(s)	1468
MboII	GAAGA	6 cut(s)	395, 647, 1177, 1258, 1483, 1510
MflI	RGATCY	2 cut(s)	844, 1167
MhlI	GDGCHC	3 cut(s)	89, 414, 1194
MlsI	TGGCCA	1 cut(s)	1493
MluCI	AATT	2 cut(s)	561, 1083
MluNI	TGGCCA	1 cut(s)	1493
Mly113I	GGCGCC	2 cut(s)	174, 422
MlyI	GAGTC	2 cut(s)	612, 761
MmeI	TCCRAC	1 cut(s)	498
Mox20I	TGGCCA	1 cut(s)	1493
MroNI	GCCGGC	3 cut(s)	113, 166, 170
MroXI	GAANNNNTTC	4 cut(s)	140, 1115, 1478, 1517
MscI	TGGCCA	1 cut(s)	1493
MseI	TTAA	1 cut(s)	567
MslI	CAYNNNNRTG	1 cut(s)	1118
Msp20I	TGGCCA	1 cut(s)	1493
MspA1I	CMGCKG	3 cut(s)	151, 489, 841
MspI	CCGG	9 cut(s)	114, 167, 171, 322, 815, 866, 888, 1213, 1253
Mva1269I	GAATGC	2 cut(s)	23, 794
MvaI	CCWGG	6 cut(s)	14, 83, 266, 293, 1415, 1506
MvnI	CGCG	4 cut(s)	238, 427, 479, 1056
MwoI	GCNNNNNNNGC	8 cut(s)	57, 84, 114, 612, 847, 1051, 1396, 1499
NaeI	GCCGGC	3 cut(s)	115, 168, 172
NarI	GGCGCC	2 cut(s)	174, 422
NciI	CCSGG	4 cut(s)	323, 815, 866, 889
NgoMIV	GCCGGC	3 cut(s)	113, 166, 170
NlaIII	CATG	4 cut(s)	301, 679, 1117, 1198
NlaIV	GGNNCC	9 cut(s)	88, 175, 346, 423, 837, 846, 1192, 1315, 1530
NmeAIII	GCCGAG	2 cut(s)	757, 1348
NmuCI	GTSAC	1 cut(s)	742
NspI	RCATGY	2 cut(s)	301, 1117
PaeR7I	CTCGAG	1 cut(s)	1522
PauI	GCGCGC	1 cut(s)	1054
PceI	AGGCCT	2 cut(s)	285, 984
PciI	ACATGT	1 cut(s)	1113
PcsI	WCGNNNNNNNCGW	5 cut(s)	48, 762, 804, 960, 1143
PctI	GAATGC	2 cut(s)	23, 794
PdiI	GCCGGC	3 cut(s)	115, 168, 172
PdmI	GAANNNNTTC	4 cut(s)	140, 1115, 1478, 1517
PfeI	GAWTC	5 cut(s)	32, 220, 990, 1327, 1458
PflFI	GACNNNGTC	1 cut(s)	621
PflMI	CCANNNNNTGG	1 cut(s)	271
PfoI	TCCNGGA	2 cut(s)	291, 1504
PkrI	GCNGC	7 cut(s)	107, 150, 208, 235, 426, 764, 840
Ple19I	CGATCG	2 cut(s)	431, 1150
PleI	GAGTC	2 cut(s)	612, 761
PluTI	GGCGCC	2 cut(s)	177, 425
PpsI	GAGTC	2 cut(s)	612, 761
Ppu21I	YACGTR	1 cut(s)	781
PpuMI	RGGWCCY	1 cut(s)	213
PscI	ACATGT	1 cut(s)	1113
PshAI	GACNNNNGTC	1 cut(s)	825
Psp1406I	AACGTT	1 cut(s)	525
Psp5II	RGGWCCY	1 cut(s)	213
Psp6I	CCWGG	6 cut(s)	12, 81, 264, 291, 1413, 1504
PspFI	CCCAGC	1 cut(s)	73
PspGI	CCWGG	6 cut(s)	12, 81, 264, 291, 1413, 1504
PspN4I	GGNNCC	9 cut(s)	88, 175, 346, 423, 837, 846, 1192, 1315, 1530
PspOMI	GGGCCC	1 cut(s)	1190
PspPPI	RGGWCCY	1 cut(s)	213
PsuI	RGATCY	2 cut(s)	844, 1167
PsyI	GACNNNGTC	1 cut(s)	621
PteI	GCGCGC	1 cut(s)	1054
PvuI	CGATCG	2 cut(s)	431, 1150
RigI	GGCCGGCC	1 cut(s)	170
RsaI	GTAC	2 cut(s)	701, 1237
RsaNI	GTAC	2 cut(s)	700, 1236
RseI	CAYNNNNRTG	1 cut(s)	1118
SaqAI	TTAA	1 cut(s)	567
SatI	GCNGC	7 cut(s)	106, 149, 207, 234, 425, 763, 839
SchI	GAGTC	2 cut(s)	612, 761
SduI	GDGCHC	3 cut(s)	89, 414, 1194
SfaNI	GCATC	1 cut(s)	325
SfoI	GGCGCC	2 cut(s)	175, 423
Sfr274I	CTCGAG	1 cut(s)	1522
SinI	GGWCC	3 cut(s)	213, 509, 1018
SlaI	CTCGAG	1 cut(s)	1522
SmiMI	CAYNNNNRTG	1 cut(s)	1118
SmlI	CTYRAG	3 cut(s)	445, 1172, 1522
SmoI	CTYRAG	3 cut(s)	445, 1172, 1522
Sse9I	AATT	2 cut(s)	561, 1083
SseBI	AGGCCT	2 cut(s)	285, 984
SspDI	GGCGCC	2 cut(s)	173, 421
SspMI	CTAG	2 cut(s)	747, 1540
StuI	AGGCCT	2 cut(s)	285, 984
StyI	CCWWGG	3 cut(s)	66, 1071, 1103
TaaI	ACNGT	7 cut(s)	91, 229, 344, 399, 457, 509, 1156
TaiI	ACGT	8 cut(s)	186, 363, 390, 470, 521, 528, 783, 1032
TaqII	GACCGA	1 cut(s)	611
TasI	AATT	2 cut(s)	561, 1083
TatI	WGTACW	1 cut(s)	699
TauI	GCSGC	7 cut(s)	108, 151, 209, 236, 427, 765, 841
TfiI	GAWTC	5 cut(s)	32, 220, 990, 1327, 1458
Tru1I	TTAA	1 cut(s)	567
Tru9I	TTAA	1 cut(s)	567
TseFI	GTSAC	1 cut(s)	742
Tsp45I	GTSAC	1 cut(s)	742
TspDTI	ATGAA	2 cut(s)	148, 149
TspGWI	ACGGA	4 cut(s)	337, 491, 545, 940
Tth111I	GACNNNGTC	1 cut(s)	621
Van91I	CCANNNNNTGG	1 cut(s)	271
VpaK11BI	GGWCC	3 cut(s)	213, 509, 1018
XagI	CCTNNNNNAGG	1 cut(s)	65
XapI	RAATTY	2 cut(s)	561, 1083
XceI	RCATGY	2 cut(s)	301, 1117
XhoI	CTCGAG	1 cut(s)	1522
XmiI	GTMKAC	2 cut(s)	351, 1203
XmnI	GAANNNNTTC	4 cut(s)	140, 1115, 1478, 1517
XspI	CTAG	2 cut(s)	747, 1540

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.