FvH4_2g38250

No description available

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb2
OG0012485
Physical Location & Seq
Forward (+)
27700461 .. 27706406
5946 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_2g38250.t2

Sequence Viewer

Length: 3129 bp
ATGACACTCGAAGATTTTTTCACCTTAACTGAGATGAAAGATGGGCTAACTGCTCCATCTCGAGTTGAGGAGCTGGTCACGTTAATGCGGAGTGAGAAAGATTCTATTGTGAATAATGCTGGTGAGGCAACCCGGCAGTGGGTTGCAGTTGCAAGTACAATTGCTGCCACAGAGAATAAAGATTGTCTTGATCTTTTTATTCAATTGGATGGAGTCTTGTTTGTTGATAAATGGCTGAAAGATGCTCAAAGTTTAGTTAATGATACAAATGAAAGTTTTGTAGAAGATTCAATAACTGCTCTGTTACGGGCACTTGAAAAGTTGCAGATTGACAATAAAAGGTCAATATCTACGGGGATCTGGAGTACTGTTGAGAGACTTCTTGGCCACAAAAGCTTGAAGGTTCAAGATCTAGCAAGACTGCTGTTTGATAGCTGGAAGCAGGATGGTGATGCAGTTGACCATGATATTGAGAATACTGGGGTTCTTTGTGGTGATGGTAGTTCTGAGCTTTCTGTACAAGAGTCCAAGCCATCTGCTTTAATTATTTCTTCTTCAGAAGTAGTTTCTACCAGCGAGAATCACCCATCAGGTACTGCTCAAGTCGAAACATTGCCGTTAAGCAGTTCGGAGGGTGTTCAGCCAGAAAGTGCTGATGTACAGATTTCAACATGTAACAAACAGTCCCCAGCCCACAAACTCTCAGAGATTGAAGACAATAAAGATAGTTCTCCGGATCCTTTGGGGTCTGTTATTTTGGAGGCTATTCAACCAAGTCCTATAAAAGATGAATCCTCTGTATGTTCTTTAGGAGAAAATGCTTTAATTGGAAGTTCTAATCTTCCTGTTGCAAAGATGAGCAGTGCTGATTTGTCAGATGACCCAAAATTGAATGAGGTGCCCAGGAATGAAGAACAGAAGCACACAGTCGATGGTTCTCCAAAGAACTTAGGTGTGACTGACATCTCTTCTGTGTCCGGACCTCCATTGGAATCTGGGGTTGTTTGTTCAGAAACTGATGCTGCAACTGCCCAGGTTTTTGTGAATGATTTACAAAAAAATGCAGATGCCAAAGAGGATGGTTTCTGTCAAAAGCTTGATCCTCTGAGTGGTGATGGACAATACAAGATCTGTATATCTGATCCACAGGTTGTGAGGGACGATACAACAGTTGTGGTGGATGGAACTACGGTTGTGTCGGACGATACAACAGCTGTGATGGATGATACAAGAAGTGTTGATCATTGCAACACTGCAGTTCAAGATAGTGACTGTTCAAACTTACCTCAGGAGTCGTCAGGTAATGGTAGTTTGTCTGGAAAAGTTGAAGATATAGAGACTTCGTCCAGGATGGATGACCTGGGTGCTGTTGACGAGGATGAAGGGCAAGCAAGTGATGAAGGGCAAGAGAGTGATGAAGGCGATGAATTGACTGTTGCTAGTGTTTTTCCCAGCAAAGTTGTTTTTCCTAGCAATATATTTGAAAAGAGAAGGTCTGATATCGATGTTGAGTATGGGATGGTTGACGCTCTTGAAGTTGCTCGACAGGTTGCTCAAGAAGTAGAAAGAGAAGTGGTGGATTACAGAGAACCATACTGCAGTTCATCTTCTGGGAAATTGTCAGGGGGTGGACTCAGACAACCTGGTAGCCCAGACTCTATAAATGAAAAGCAAGACCCACTAACTGAAGTTGCACCAAAGGATGTGCCAGTTGAACAAGTTCATTCTGTGGAGGCAAATCCTGAGAAGGACGTAGTTGAGTCGGAACATCAGGAAATGGTACCTGAACATTCCATTCATGACATGGAGTCATCTCAAGTGACTGAAACGGCTCAAGAACCAGAAGTTAACTCAGAAAAGGGTCTCTGTGGTTTTGATCTAAATGAAGAAGTGAGTTCTGATGAGATGGATTGTTCAACAAATCCCGTCTCTGCTCCAATTCCTTTTTCCAGACCACCACCAGCTGCTGATCTACCTGTGGCCCCTTTGCAGTTTGAAGGGGCAATTGGATTGAAAGGCTCTCTTGGCAATAGTGCTTTTCGTCGAGCATCTCCTCGCAGGTTTTTAGAGTCTGAGAAAAATCTTTCTGCAGGGGCCACCACTGATAGCTCAAAGCAGAGGTCAGACTACCAGTGTTTAGATTTGAATGTTGCTCTTGGAGGAGATGATTTGGAAAAACAAATTCCATTGTCGTCTGGCCTCCCTTCTGGGGAATCTTCAGGGGAAGTGAGCCAAAGTAGACTGGGTAGGCCCAACTTGGATTTGAACCGTATTGATGATGATGGTGATGTTCTTCCATTGAACTTAAGAGTGGAAGGACAGTTCTTGTATAACCGAAATCCCCGTCGCAGCCCATCTCCTGCTTCATCGTCATCATCAATGCAGCCTTTGATGAGGAATTTTGATTTGAATGACAGGCCTTTTTTTCTTAATGATTCTATTGATCAAGGGCATGGTAAGTCTCCTCAAAGTGCAGCTGCATATAGAGGGCAGCTAGATGGTTCAGTTATTTCTATCATGGGTACCAGAGTGGAGATTAAAAGAAATGATGCTCCTCAAACCTTGTCCCTATCAAATGGCAAGGGTATTATTGAGACTGCAGGAGATCCCAACTTGGCAAGAGCGGGAAGTCTTTTAGAGTTGGGTTCAAGGGTTTCTTACACCAACTCTCCTATTTTTGGGTACAATGGACTGGCTGCAGGGCCCACAATGTCTTTCTCGTCAACCATGTATGGACCTGGTGGAGCAATCCCCTACATGGTGGATTCAAGAGGATCCCCTGTTCCGGTTGTGCCTCAAGTTATGGGCTCTGCATCAGCTGTTCCACCTCCGTTCTCTCAGTCACCATTTCTCATGAATATGAATGGCATGCAACCGGCTCTAAATGGTGCTGGGCCATCGCGTCCCAGTTTTGATCTAAACTCTGGCTTCATGGTTGAGAGTGGCAATAGAGATTCGGGCTTGAGGCACCTTTTCATTCATGGCCAAGGTGGATCTATGGATGAGCATCTCAGGAACAGCTTACAACCCCCTTCTAGTTCTAATGTTGGTGGAAAAAGGAAAGAACCAGAAGGTGGTTGGGAACCTTACCCGTTTAGTTACAGACATCAGCAACCCCCATGGAGATAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Med26

Protein Analysis

1043

Amino Acids

111.82

Weight (kDa)

4.43

Isoelectric Point (pI)

57.08

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Med26 PF08711 101 - 148 9.1e-06 TFIIS helical bundle-like domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0012485)

Species Orthologous Gene IDs
arabidopsis_thaliana AT4G24200 AT4G24200
fragaria_vesca FvH4_2g38250 FvH4_2g38250
malus_domestica MD08G1010900.v1.1 MD15G1010900.v1.1
prunus_persica Prupe.1G363500_v2.0.a1
pyrus_communis pycom08g00970 pycom15g00930
rosa_chinensis RchiOBHm_Chr6g0303121
rosa_laevigata RLG00000011106
rosa_multiflora Rmu_sc0008911.1_g000006
rosa_roxburghii Rroxscaffold_7G00165000
rosa_rugosa Rorug06G0319200
rosa_samantha Rh6AG433200 Rh6BG469500 Rh6CG445500 Rh6DG432300
rosa_wichuraiana Rw6G037550

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 2049
Acc65I GGTACC 2 cut(s) 1780, 2522
AccB1I GGYRCC 4 cut(s) 898, 1780, 2522, 2967
AccB7I CCANNNNNTGG 1 cut(s) 3074
AccBSI CCGCTC 1 cut(s) 2624
AccI GTMKAC 1 cut(s) 2239
AccII CGCG 1 cut(s) 2902
AccIII TCCGGA 2 cut(s) 733, 977
AciI CCGC 2 cut(s) 88, 2624
AclWI GGATC 9 cut(s) 365, 731, 744, 1094, 1136, 2600, 2769, 2782, 3001
AcoI YGGCCR 2 cut(s) 385, 2983
AcsI RAATTY 2 cut(s) 2181, 2398
AcuI CTGAAG 3 cut(s) 540, 1707, 2202
AfaI GTAC 8 cut(s) 157, 367, 519, 595, 660, 1782, 2524, 2684
AfiI CCNNNNNNNGG 8 cut(s) 138, 139, 1109, 2209, 2678, 2758, 2785, 3074
AflII CTTAAG 1 cut(s) 2305
AflIII ACRYGT 1 cut(s) 671
AhdI GACNNNNNGTC 1 cut(s) 1807
AjnI CCWGG 6 cut(s) 902, 1032, 1346, 1359, 1642, 2737
AjuI GAANNNNNNNTTGG 4 cut(s) 1930, 1962, 1989, 2021
AloI GAACNNNNNNTCC 4 cut(s) 2306, 2338, 2766, 2798
Alw26I GTCTC 6 cut(s) 370, 1331, 1868, 1933, 2466, 2588
AlwI GGATC 9 cut(s) 365, 731, 744, 1094, 1136, 2600, 2769, 2782, 3001
AlwNI CAGNNNCTG 3 cut(s) 596, 1016, 1967
Ama87I CYCGRG 1 cut(s) 60
Aor13HI TCCGGA 2 cut(s) 733, 977
AoxI GGCC 9 cut(s) 385, 1980, 2094, 2197, 2249, 2417, 2702, 2894, 2983
ApaI GGGCCC 1 cut(s) 2706
ApeKI GCWGC 9 cut(s) 164, 1022, 1964, 2349, 2383, 2474, 2477, 2491, 2696
ApoI RAATTY 2 cut(s) 2181, 2398
ArsI GACNNNNNNTTYG 4 cut(s) 2105, 2137, 2329, 2361
Asp700I GAANNNNTTC 1 cut(s) 1719
Asp718I GGTACC 2 cut(s) 1780, 2522
AspS9I GGNCC 8 cut(s) 980, 1981, 2094, 2250, 2702, 2703, 2735, 2894
AsuC2I CCSGG 1 cut(s) 133
AsuHPI GGTGA 8 cut(s) 13, 134, 461, 506, 575, 1124, 2297, 2835
AvaI CYCGRG 1 cut(s) 60
AvaII GGWCC 2 cut(s) 980, 2735
AxyI CCTNAGG 1 cut(s) 1287
BaeGI GKGCMC 3 cut(s) 313, 903, 2706
BaeI ACNNNNGTAYC 4 cut(s) 1155, 1188, 2514, 2547
BalI TGGCCA 2 cut(s) 387, 2985
BamHI GGATCC 2 cut(s) 736, 2774
BanI GGYRCC 4 cut(s) 898, 1780, 2522, 2967
BanII GRGCYC 2 cut(s) 2706, 2810
BbsI GAAGAC 1 cut(s) 720
BbvI GCAGC 9 cut(s) 151, 1009, 1951, 2361, 2395, 2464, 2486, 2503, 2683
BceAI ACGGC 2 cut(s) 601, 1845
BciT130I CCWGG 6 cut(s) 904, 1034, 1348, 1361, 1644, 2739
BclI TGATCA 2 cut(s) 1240, 2443
BcnI CCSGG 1 cut(s) 133
BcoDI GTCTC 6 cut(s) 370, 1331, 1868, 1933, 2466, 2588
BfaI CTAG 5 cut(s) 413, 1440, 1470, 2495, 3036
BfmI CTRYAG 5 cut(s) 1254, 1597, 2088, 2598, 2697
BfrI CTTAAG 1 cut(s) 2305
BfuAI ACCTGC 1 cut(s) 2049
BglII AGATCT 2 cut(s) 409, 1128
BisI GCNGC 9 cut(s) 165, 1023, 1965, 2350, 2384, 2475, 2478, 2492, 2697
BlsI GCNGC 9 cut(s) 166, 1024, 1966, 2351, 2385, 2476, 2479, 2493, 2698
BmcAI AGTACT 1 cut(s) 367
Bme1390I CCNGG 7 cut(s) 133, 904, 1034, 1348, 1361, 1644, 2739
Bme18I GGWCC 2 cut(s) 980, 2735
BmeRI GACNNNNNGTC 1 cut(s) 1807
BmeT110I CYCGRG 1 cut(s) 60
BmgT120I GGNCC 8 cut(s) 980, 1981, 2094, 2250, 2702, 2703, 2735, 2894
BmrFI CCNGG 7 cut(s) 133, 904, 1034, 1348, 1361, 1644, 2739
BmrI ACTGGG 3 cut(s) 489, 2252, 2901
BmsI GCATC 8 cut(s) 232, 442, 1009, 1057, 2057, 2539, 2822, 3016
BmuI ACTGGG 3 cut(s) 489, 2252, 2901
BpiI GAAGAC 1 cut(s) 720
BpmI CTGGAG 1 cut(s) 382
BpuEI CTTGAG 6 cut(s) 585, 1539, 1800, 1818, 2781, 2983
BpuMI CCSGG 1 cut(s) 133
Bsa29I ATCGAT 1 cut(s) 1503
BsaBI GATNNNNATC 1 cut(s) 3006
BsaI GGTCTC 1 cut(s) 1868
BsaJI CCNNGG 5 cut(s) 902, 1032, 1360, 2986, 3119
BsaWI WCCGGW 3 cut(s) 733, 977, 2785
BsaXI ACNNNNNCTCC 2 cut(s) 2653, 2683
Bsc4I CCNNNNNNNGG 8 cut(s) 138, 139, 1109, 2209, 2678, 2758, 2785, 3074
Bse118I RCCGGY 1 cut(s) 2875
Bse1I ACTGG 6 cut(s) 484, 1709, 2131, 2247, 2697, 2907
Bse21I CCTNAGG 1 cut(s) 1287
Bse3DI GCAATG 2 cut(s) 611, 1243
Bse8I GATNNNNATC 1 cut(s) 3006
BseAI TCCGGA 2 cut(s) 733, 977
BseBI CCWGG 6 cut(s) 904, 1034, 1348, 1361, 1644, 2739
BseCI ATCGAT 1 cut(s) 1503
BseDI CCNNGG 5 cut(s) 902, 1032, 1360, 2986, 3119
BseJI GATNNNNATC 1 cut(s) 3006
BseLI CCNNNNNNNGG 8 cut(s) 138, 139, 1109, 2209, 2678, 2758, 2785, 3074
BseMI GCAATG 2 cut(s) 611, 1243
BseNI ACTGG 6 cut(s) 484, 1709, 2131, 2247, 2697, 2907
BseRI GAGGAG 5 cut(s) 83, 2043, 2175, 2454, 2544
BseSI GKGCMC 3 cut(s) 313, 903, 2706
BseXI GCAGC 9 cut(s) 151, 1009, 1951, 2361, 2395, 2464, 2486, 2503, 2683
BseYI CCCAGC 3 cut(s) 688, 1451, 2891
BsgI GTGCAG 1 cut(s) 2493
Bsh1236I CGCG 1 cut(s) 2902
BshFI GGCC 9 cut(s) 387, 1982, 2096, 2199, 2251, 2419, 2704, 2896, 2985
BshNI GGYRCC 4 cut(s) 898, 1780, 2522, 2967
BshVI ATCGAT 1 cut(s) 1503
BsiHKCI CYCGRG 1 cut(s) 60
BsiSI CCGG 5 cut(s) 133, 734, 978, 2786, 2876
BslFI GGGAC 4 cut(s) 670, 1172, 2551, 2889
BslI CCNNNNNNNGG 8 cut(s) 138, 139, 1109, 2209, 2678, 2758, 2785, 3074
BsmAI GTCTC 6 cut(s) 370, 1331, 1868, 1933, 2466, 2588
BsmBI CGTCTC 1 cut(s) 1933
BsmFI GGGAC 4 cut(s) 670, 1172, 2551, 2889
BsnI GGCC 9 cut(s) 387, 1982, 2096, 2199, 2251, 2419, 2704, 2896, 2985
Bso31I GGTCTC 1 cut(s) 1868
BsoBI CYCGRG 1 cut(s) 60
Bsp120I GGGCCC 1 cut(s) 2702
Bsp1286I GDGCHC 4 cut(s) 313, 903, 2706, 2810
Bsp13I TCCGGA 2 cut(s) 733, 977
Bsp1407I TGTACA 2 cut(s) 517, 658
Bsp19I CCATGG 1 cut(s) 3119
BspACI CCGC 2 cut(s) 88, 2624
BspANI GGCC 9 cut(s) 387, 1982, 2096, 2199, 2251, 2419, 2704, 2896, 2985
BspDI ATCGAT 1 cut(s) 1503
BspEI TCCGGA 2 cut(s) 733, 977
BspFNI CGCG 1 cut(s) 2902
BspHI TCATGA 2 cut(s) 1798, 2853
BspMAI CTGCAG 5 cut(s) 1258, 1601, 2092, 2602, 2701
BspMI ACCTGC 1 cut(s) 2049
BspPI GGATC 9 cut(s) 365, 731, 744, 1094, 1136, 2600, 2769, 2782, 3001
BspT107I GGYRCC 4 cut(s) 898, 1780, 2522, 2967
BspTI CTTAAG 1 cut(s) 2305
BspTNI GGTCTC 1 cut(s) 1868
BsrBI CCGCTC 1 cut(s) 2624
BsrDI GCAATG 2 cut(s) 611, 1243
BsrFI RCCGGY 1 cut(s) 2875
BsrGI TGTACA 2 cut(s) 517, 658
BsrI ACTGG 6 cut(s) 484, 1709, 2131, 2247, 2697, 2907
BssAI RCCGGY 1 cut(s) 2875
BssECI CCNNGG 5 cut(s) 902, 1032, 1360, 2986, 3119
BssT1I CCWWGG 2 cut(s) 2986, 3119
Bst2UI CCWGG 6 cut(s) 904, 1034, 1348, 1361, 1644, 2739
Bst4CI ACNGT 9 cut(s) 370, 684, 928, 1171, 1192, 1274, 1435, 2270, 2322
Bst6I CTCTTC 1 cut(s) 973
BstAFI CTTAAG 1 cut(s) 2305
BstAUI TGTACA 2 cut(s) 517, 658
BstC8I GCNNGC 2 cut(s) 1389, 2870
BstDSI CCRYGG 1 cut(s) 3119
BstFNI CGCG 1 cut(s) 2902
BstMAI GTCTC 6 cut(s) 370, 1331, 1868, 1933, 2466, 2588
BstMWI GCNNNNNNNGC 4 cut(s) 125, 393, 1028, 2025
BstNI CCWGG 6 cut(s) 904, 1034, 1348, 1361, 1644, 2739
BstNSI RCATGY 2 cut(s) 675, 2872
BstSCI CCNGG 7 cut(s) 131, 902, 1032, 1346, 1359, 1642, 2737
BstSFI CTRYAG 5 cut(s) 1254, 1597, 2088, 2598, 2697
BstSLI GKGCMC 3 cut(s) 313, 903, 2706
BstUI CGCG 1 cut(s) 2902
BstV1I GCAGC 9 cut(s) 151, 1009, 1951, 2361, 2395, 2464, 2486, 2503, 2683
BstV2I GAAGAC 1 cut(s) 720
BstX2I RGATCY 7 cut(s) 357, 409, 736, 1128, 2605, 2774, 2993
BstYI RGATCY 7 cut(s) 357, 409, 736, 1128, 2605, 2774, 2993
Bsu15I ATCGAT 1 cut(s) 1503
Bsu36I CCTNAGG 1 cut(s) 1287
BsuRI GGCC 9 cut(s) 387, 1982, 2096, 2199, 2251, 2419, 2704, 2896, 2985
BsuTUI ATCGAT 1 cut(s) 1503
BtgI CCRYGG 1 cut(s) 3119
BtgZI GCGATG 2 cut(s) 1437, 2883
BtsI GCAGTG 3 cut(s) 143, 868, 1251
BtsIMutI CAGTG 5 cut(s) 143, 868, 1251, 2100, 2138
BveI ACCTGC 1 cut(s) 2049
Cac8I GCNNGC 2 cut(s) 1389, 2870
CaiI CAGNNNCTG 3 cut(s) 596, 1016, 1967
CciI TCATGA 2 cut(s) 1798, 2853
Cfr10I RCCGGY 1 cut(s) 2875
Cfr13I GGNCC 8 cut(s) 980, 1981, 2094, 2250, 2702, 2703, 2735, 2894
ClaI ATCGAT 1 cut(s) 1503
CseI GACGC 2 cut(s) 1535, 2891
CsiI ACCWGGT 2 cut(s) 1642, 2737
Csp6I GTAC 8 cut(s) 156, 366, 518, 594, 659, 1781, 2523, 2683
CspCI CAANNNNNGTGG 2 cut(s) 1155, 1190
CviQI GTAC 8 cut(s) 156, 366, 518, 594, 659, 1781, 2523, 2683
DriI GACNNNNNGTC 1 cut(s) 1807
EaeI YGGCCR 2 cut(s) 385, 2983
Eam1104I CTCTTC 1 cut(s) 973
Eam1105I GACNNNNNGTC 1 cut(s) 1807
EarI CTCTTC 1 cut(s) 973
Eco130I CCWWGG 2 cut(s) 2986, 3119
Eco147I AGGCCT 1 cut(s) 2419
Eco24I GRGCYC 2 cut(s) 2706, 2810
Eco31I GGTCTC 1 cut(s) 1868
Eco32I GATATC 1 cut(s) 1501
Eco47I GGWCC 2 cut(s) 980, 2735
Eco57I CTGAAG 3 cut(s) 540, 1707, 2202
Eco81I CCTNAGG 1 cut(s) 1287
Eco88I CYCGRG 1 cut(s) 60
EcoO109I RGGNCCY 1 cut(s) 2702
EcoRII CCWGG 6 cut(s) 902, 1032, 1346, 1359, 1642, 2737
EcoRV GATATC 1 cut(s) 1501
EcoT14I CCWWGG 2 cut(s) 2986, 3119
EcoT38I GRGCYC 2 cut(s) 2706, 2810
ErhI CCWWGG 2 cut(s) 2986, 3119
Esp3I CGTCTC 1 cut(s) 1933
FalI AAGNNNNNCTT 6 cut(s) 171, 203, 2007, 2039, 2641, 2673
FaqI GGGAC 4 cut(s) 670, 1172, 2551, 2889
FauI CCCGC 1 cut(s) 2617
FbaI TGATCA 2 cut(s) 1240, 2443
FblI GTMKAC 1 cut(s) 2239
Fnu4HI GCNGC 9 cut(s) 165, 1023, 1965, 2350, 2384, 2475, 2478, 2492, 2697
FriOI GRGCYC 2 cut(s) 2706, 2810
Fsp4HI GCNGC 9 cut(s) 165, 1023, 1965, 2350, 2384, 2475, 2478, 2492, 2697
FspBI CTAG 5 cut(s) 413, 1440, 1470, 2495, 3036
GluI GCNGC 9 cut(s) 165, 1023, 1965, 2350, 2384, 2475, 2478, 2492, 2697
GsaI CCCAGC 3 cut(s) 692, 1455, 2895
GsuI CTGGAG 1 cut(s) 382
HaeIII GGCC 9 cut(s) 387, 1982, 2096, 2199, 2251, 2419, 2704, 2896, 2985
HapII CCGG 5 cut(s) 133, 734, 978, 2786, 2876
HgaI GACGC 2 cut(s) 1535, 2891
HincII GTYRAC 5 cut(s) 460, 1372, 1525, 1849, 2724
HindII GTYRAC 5 cut(s) 460, 1372, 1525, 1849, 2724
HindIII AAGCTT 2 cut(s) 394, 1094
HpaI GTTAAC 1 cut(s) 1849
HpaII CCGG 5 cut(s) 133, 734, 978, 2786, 2876
HphI GGTGA 8 cut(s) 13, 134, 461, 506, 575, 1124, 2297, 2835
Hpy166II GTNNAC 7 cut(s) 460, 1372, 1525, 1631, 1849, 2240, 2724
Hpy8I GTNNAC 7 cut(s) 460, 1372, 1525, 1631, 1849, 2240, 2724
Hpy99I CGWCG 2 cut(s) 2046, 2349
HpyCH4III ACNGT 9 cut(s) 370, 684, 928, 1171, 1192, 1274, 1435, 2270, 2322
HpyCH4IV ACGT 2 cut(s) 80, 1752
HpyF10VI GCNNNNNNNGC 4 cut(s) 125, 393, 1028, 2025
HpySE526I ACGT 2 cut(s) 80, 1752
Kpn2I TCCGGA 2 cut(s) 733, 977
KpnI GGTACC 2 cut(s) 1784, 2526
Ksp22I TGATCA 2 cut(s) 1240, 2443
KspAI GTTAAC 1 cut(s) 1849
LmnI GCTCC 5 cut(s) 58, 70, 1939, 2557, 2744
Lsp1109I GCAGC 9 cut(s) 151, 1009, 1951, 2361, 2395, 2464, 2486, 2503, 2683
LweI GCATC 8 cut(s) 232, 442, 1009, 1057, 2057, 2539, 2822, 3016
MabI ACCWGGT 2 cut(s) 1642, 2737
MaeI CTAG 5 cut(s) 413, 1440, 1470, 2495, 3036
MaeII ACGT 2 cut(s) 80, 1752
MaeIII GTNAC 8 cut(s) 76, 303, 674, 955, 1268, 1819, 2841, 3098
MbiI CCGCTC 1 cut(s) 2624
MfeI CAATTG 3 cut(s) 159, 203, 2004
MflI RGATCY 7 cut(s) 357, 409, 736, 1128, 2605, 2774, 2993
MhlI GDGCHC 4 cut(s) 313, 903, 2706, 2810
MlsI TGGCCA 2 cut(s) 387, 2985
MluNI TGGCCA 2 cut(s) 387, 2985
MlyI GAGTC 8 cut(s) 222, 533, 1301, 1626, 1649, 1769, 1817, 2078
MmeI TCCRAC 2 cut(s) 1179, 1743
Mox20I TGGCCA 2 cut(s) 387, 2985
MroI TCCGGA 2 cut(s) 733, 977
MroXI GAANNNNTTC 1 cut(s) 1719
MscI TGGCCA 2 cut(s) 387, 2985
MslI CAYNNNNRTG 2 cut(s) 83, 2858
Msp20I TGGCCA 2 cut(s) 387, 2985
MspA1I CMGCKG 4 cut(s) 1214, 1964, 2477, 2819
MspCI CTTAAG 1 cut(s) 2305
MspI CCGG 5 cut(s) 133, 734, 978, 2786, 2876
MspR9I CCNGG 7 cut(s) 133, 904, 1034, 1348, 1361, 1644, 2739
MunI CAATTG 3 cut(s) 159, 203, 2004
MvaI CCWGG 6 cut(s) 904, 1034, 1348, 1361, 1644, 2739
MvnI CGCG 1 cut(s) 2902
MwoI GCNNNNNNNGC 4 cut(s) 125, 393, 1028, 2025
NciI CCSGG 1 cut(s) 133
NcoI CCATGG 1 cut(s) 3119
NmuCI GTSAC 5 cut(s) 76, 955, 1268, 1819, 2841
NspI RCATGY 2 cut(s) 675, 2872
PaeI GCATGC 1 cut(s) 2872
PaeR7I CTCGAG 1 cut(s) 60
PagI TCATGA 2 cut(s) 1798, 2853
PceI AGGCCT 1 cut(s) 2419
PciI ACATGT 1 cut(s) 671
PdmI GAANNNNTTC 1 cut(s) 1719
PfeI GAWTC 9 cut(s) 101, 287, 580, 791, 992, 2213, 2435, 2765, 2954
PflFI GACNNNGTC 1 cut(s) 1342
PflMI CCANNNNNTGG 1 cut(s) 3074
PfoI TCCNGGA 1 cut(s) 1346
PkrI GCNGC 9 cut(s) 166, 1024, 1966, 2351, 2385, 2476, 2479, 2493, 2698
PleI GAGTC 8 cut(s) 221, 532, 1300, 1626, 1649, 1768, 1816, 2077
PpsI GAGTC 8 cut(s) 221, 532, 1300, 1626, 1649, 1768, 1816, 2077
PscI ACATGT 1 cut(s) 671
Psp6I CCWGG 6 cut(s) 902, 1032, 1346, 1359, 1642, 2737
PspFI CCCAGC 3 cut(s) 688, 1451, 2891
PspGI CCWGG 6 cut(s) 902, 1032, 1346, 1359, 1642, 2737
PspOMI GGGCCC 1 cut(s) 2702
PspPI GGNCC 8 cut(s) 980, 1981, 2094, 2250, 2702, 2703, 2735, 2894
PstI CTGCAG 5 cut(s) 1258, 1601, 2092, 2602, 2701
PstNI CAGNNNCTG 3 cut(s) 596, 1016, 1967
PsuI RGATCY 7 cut(s) 357, 409, 736, 1128, 2605, 2774, 2993
PsyI GACNNNGTC 1 cut(s) 1342
PvuII CAGCTG 4 cut(s) 1214, 1964, 2477, 2819
RsaI GTAC 8 cut(s) 157, 367, 519, 595, 660, 1782, 2524, 2684
RsaNI GTAC 8 cut(s) 156, 366, 518, 594, 659, 1781, 2523, 2683
RseI CAYNNNNRTG 2 cut(s) 83, 2858
SatI GCNGC 9 cut(s) 165, 1023, 1965, 2350, 2384, 2475, 2478, 2492, 2697
Sau96I GGNCC 8 cut(s) 980, 1981, 2094, 2250, 2702, 2703, 2735, 2894
ScaI AGTACT 1 cut(s) 367
SchI GAGTC 8 cut(s) 222, 533, 1301, 1626, 1649, 1769, 1817, 2078
ScrFI CCNGG 7 cut(s) 133, 904, 1034, 1348, 1361, 1644, 2739
SduI GDGCHC 4 cut(s) 313, 903, 2706, 2810
SexAI ACCWGGT 2 cut(s) 1642, 2737
SfaNI GCATC 8 cut(s) 232, 442, 1009, 1057, 2057, 2539, 2822, 3016
SfcI CTRYAG 5 cut(s) 1254, 1597, 2088, 2598, 2697
Sfr274I CTCGAG 1 cut(s) 60
SinI GGWCC 2 cut(s) 980, 2735
SlaI CTCGAG 1 cut(s) 60
SmiMI CAYNNNNRTG 2 cut(s) 83, 2858
SmlI CTYRAG 8 cut(s) 60, 600, 1554, 1815, 1833, 2305, 2796, 2962
SmoI CTYRAG 8 cut(s) 60, 600, 1554, 1815, 1833, 2305, 2796, 2962
SphI GCATGC 1 cut(s) 2872
SseBI AGGCCT 1 cut(s) 2419
SsiI CCGC 2 cut(s) 88, 2624
SspMI CTAG 5 cut(s) 413, 1440, 1470, 2495, 3036
StuI AGGCCT 1 cut(s) 2419
StyD4I CCNGG 7 cut(s) 131, 902, 1032, 1346, 1359, 1642, 2737
StyI CCWWGG 2 cut(s) 2986, 3119
TaaI ACNGT 9 cut(s) 370, 684, 928, 1171, 1192, 1274, 1435, 2270, 2322
TaiI ACGT 2 cut(s) 83, 1755
TaqI TCGA 7 cut(s) 9, 61, 606, 930, 1503, 1543, 2044
TatI WGTACW 4 cut(s) 155, 365, 517, 658
TfiI GAWTC 9 cut(s) 101, 287, 580, 791, 992, 2213, 2435, 2765, 2954
TscAI CASTG 5 cut(s) 143, 868, 1258, 2107, 2138
TseFI GTSAC 5 cut(s) 76, 955, 1268, 1819, 2841
TseI GCWGC 9 cut(s) 164, 1022, 1964, 2349, 2383, 2474, 2477, 2491, 2696
Tsp45I GTSAC 5 cut(s) 76, 955, 1268, 1819, 2841
TspGWI ACGGA 1 cut(s) 2820
TspRI CASTG 5 cut(s) 143, 868, 1258, 2107, 2138
Tth111I GACNNNGTC 1 cut(s) 1342
Van91I CCANNNNNTGG 1 cut(s) 3074
Vha464I CTTAAG 1 cut(s) 2305
VpaK11BI GGWCC 2 cut(s) 980, 2735
XapI RAATTY 2 cut(s) 2181, 2398
XceI RCATGY 2 cut(s) 675, 2872
XcmI CCANNNNNNNNNTGG 2 cut(s) 1801, 3075
XhoI CTCGAG 1 cut(s) 60
XmiI GTMKAC 1 cut(s) 2239
XmnI GAANNNNTTC 1 cut(s) 1719
XspI CTAG 5 cut(s) 413, 1440, 1470, 2495, 3036
ZrmI AGTACT 1 cut(s) 367
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.